LOCUS       DBNCDC010000001        34051 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_221_length_34051_cov_8.564008, whole genome shotgun sequence.
ACCESSION   DBNCDC010000001 DBNCDC010000000
VERSION     DBNCDC010000001.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 34051)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 34051)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34051
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_221_length_34051_cov_8.564008"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /gene="sstT"
                     /locus_tag="AC04VQ_00005"
     CDS             complement(<1..997)
                     /gene="sstT"
                     /locus_tag="AC04VQ_00005"
                     /inference="COORDINATES: protein motif:HMM:NF010151.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HIO9552665.1"
                     /translation="MKKLFIKYRNTNLILRIVIGLIIGSTVGVLCNLAVNHQGGLHEN
                     EIVVNCLGALAVTFQVFGNLFIGGLRAIAPILVFFLITSSLMGAKKAGSKTVQKTMGL
                     YAIGTFSAAFIAVVINVLFPVTLALPSEVDISQYSAPEGMGDIVVNIFTSIVANPIDA
                     IASANFLGVIFWSILFGIIARPILPERIKEGFQKIAEVLTGIVKIIIDFAPFGIAGLV
                     FTAILDNGIGIFTDYGLLLCVYLSILIFIAFGFNALIVGLVMRRNPYPLIFRTIKDVA
                     ITAFFMRSSAANIPVNMKFCDELHLDEKTYSITIPIGSTINMEGASITIITLTLAC"
     gene            complement(997..1575)
                     /locus_tag="AC04VQ_00010"
     CDS             complement(997..1575)
                     /locus_tag="AC04VQ_00010"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009608477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HIO9552666.1"
                     /translation="MKLLKDKIKKLGQIKDGDVLKVDEFLNHQIDTKLVDKMAEEFIK
                     RFKGIKFTKVLTIETSGIAVAYPVAQKLGIPLVFAKKKDSVNINGEKYSSKVISYTHF
                     REYDETVSKKFLSNKDHILIIDDFLAKGSPLRGLLSICIQAGAKVDGIGIAIEKVYQN
                     GGNELRDEGYRVESLVKILEIDEKTNKIKFGD"
     gene            complement(1589..1663)
                     /locus_tag="AC04VQ_00015"
     tRNA            complement(1589..1663)
                     /locus_tag="AC04VQ_00015"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1629..1631),aa:Ile,seq:cat)
     gene            complement(2001..2195)
                     /locus_tag="AC04VQ_00020"
     CDS             complement(2001..2195)
                     /locus_tag="AC04VQ_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552667.1"
                     /translation="MDKDSQNAIAEAEATHNFNLESYIEADKKFMLLHSCDDFTNVVD
                     APECFRRGKAKGRDNVYKTA"
     gene            complement(2406..2639)
                     /locus_tag="AC04VQ_00025"
     CDS             complement(2406..2639)
                     /locus_tag="AC04VQ_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552668.1"
                     /translation="MPVIEGYMPYLGFKTYFRIAGKGNEAKAPLLVLHGGPGGSHNTL
                     EVLDVLSLIEDRQIIYYDQLGCGKSYVEGHTEL"
     gene            complement(2729..3079)
                     /locus_tag="AC04VQ_00030"
     CDS             complement(2729..3079)
                     /locus_tag="AC04VQ_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552669.1"
                     /translation="MASFCSKPKFDNFKENLRMTKGVIVRHMILPGHIQNSKDVLKML
                     FDEFGNSIKFSIMNQYTPVLDKESEVVKNHPELLKTVSQEDYEEVLNFADNLGIEDYY
                     WQNGLTCSESFIPN"
     gene            complement(3179..3457)
                     /locus_tag="AC04VQ_00035"
     CDS             complement(3179..3457)
                     /locus_tag="AC04VQ_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552670.1"
                     /translation="MKCVYCQNREISGVGNVCGKETNIDELVDMMLDLQNQGAMNINL
                     VTATHYRSHLISAVRLAKQKGLCLPIVWNTSGYETLSSVYALNDTVDI"
     gene            complement(3688..4569)
                     /gene="nadE"
                     /locus_tag="AC04VQ_00040"
                     /pseudo
     CDS             complement(3688..4569)
                     /gene="nadE"
                     /locus_tag="AC04VQ_00040"
                     /inference="COORDINATES: protein motif:HMM:NF014586.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
     gene            complement(4579..4839)
                     /locus_tag="AC04VQ_00045"
     CDS             complement(4579..4839)
                     /locus_tag="AC04VQ_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013334.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase/urease maturation nickel
                     metallochaperone HypA"
                     /protein_id="HIO9552671.1"
                     /translation="MHELGIATGVLDSVVSVAKEQNCLRVKQIDVVVGKKAAIIDEMF
                     EEAFNALKELDDYKLCKEAIIKTQLSEGRDVYIDKIEVEQED"
     gene            complement(4851..5633)
                     /gene="tatC"
                     /locus_tag="AC04VQ_00050"
                     /pseudo
     CDS             complement(4851..5633)
                     /gene="tatC"
                     /locus_tag="AC04VQ_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013097.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
     gene            complement(5633..5929)
                     /locus_tag="AC04VQ_00055"
     CDS             complement(5633..5929)
                     /locus_tag="AC04VQ_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014470.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIO9552672.1"
                     /translation="MFGIGGFELFIIVVFIFVIFGPKKMPEVTRYAGLAIKKFRKAKA
                     EMDKVVQEELVDPVMKVANEPDPPKDKPKPKDIKSESKNNKSQTNATAEKKGDK"
     gene            complement(5954..6190)
                     /locus_tag="AC04VQ_00060"
     CDS             complement(5954..6190)
                     /locus_tag="AC04VQ_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CASC3 protein CASC3"
                     /protein_id="HIO9552673.1"
                     /translation="MSKRRTMTPKQKQAKIDAAKKSEEAAKRAQARGERRKKIITTFF
                     CVILVLGLGIPTVSLTVCSQMNQQSAQNSEAEAA"
     gene            complement(6275..6802)
                     /locus_tag="AC04VQ_00065"
     CDS             complement(6275..6802)
                     /locus_tag="AC04VQ_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIO9552674.1"
                     /translation="MIILLAGIFVNILFAFIVFVVLYSFVGFDVQQQSTGEITHINMT
                     PIEAIQYGINYLWAVICAVVNLFNPATMAETVSNSTSLIGIAVISKSAAEAGICAFLE
                     FMAMISISLGIMNLLPIPPLDGGRFLIEIYQVITKKNVSVRAINIISFIGIALILLLF
                     VVVMNQDIQRFILGN"
     gene            complement(6974..7336)
                     /locus_tag="AC04VQ_00070"
     CDS             complement(6974..7336)
                     /locus_tag="AC04VQ_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIO9552675.1"
                     /translation="MDILITILCCLIVLSVLVFLHEGGHFVTARLFGVRVSEFMLGLP
                     GPRLSFKHNDTRYGVTCVPLGGYARVCGMEPGNMQPFLAETLEFIHKSGKTTIPLVAH
                     ELKISKDEAEDALDELVD"
     gene            7438..7626
                     /locus_tag="AC04VQ_00075"
     CDS             7438..7626
                     /locus_tag="AC04VQ_00075"
                     /inference="COORDINATES: protein motif:HMM:NF026048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIO9552676.1"
                     /translation="MPRPIINTEDCTACGSCVEVCPNGVLEIEDTAVSVVNEDECTAC
                     ESCMEECPMGAITKIEED"
     gene            complement(7674..8231)
                     /locus_tag="AC04VQ_00080"
     CDS             complement(7674..8231)
                     /locus_tag="AC04VQ_00080"
                     /inference="COORDINATES: protein motif:HMM:NF022779.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HIO9552677.1"
                     /translation="MKKSKVVYKYKDFLNYIDIPKRGDEETWDNYTEQLENPVEITIN
                     KNNKIYYNYCHALLFSDSYSIKIKKKINNPLFLTYTSSVIYNVDEFIETEDIKLNDNL
                     DIFCDDAETVFYILTPQVIEAINKLIEVTGKRVIIFFLNGWMHLFVLNSNLLNHLTKR
                     NIQKNYQKVQDIALAVAKIQNLINV"
     gene            complement(8487..8723)
                     /locus_tag="AC04VQ_00085"
     CDS             complement(8487..8723)
                     /locus_tag="AC04VQ_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552678.1"
                     /translation="MSSADSYINKRGNRKPNIRSKRELIIYGIVMFSVYFIITFILSN
                     IMLADAEESRKLPGTIFMSFMIGFIGATLCPVTL"
     gene            complement(8907..9404)
                     /locus_tag="AC04VQ_00090"
     CDS             complement(8907..9404)
                     /locus_tag="AC04VQ_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIO9552679.1"
                     /translation="MKIAPSIMCSAPWDVKTYVEKFEETGIPLIHYDVMDGNYVPNIS
                     VGTGIYADIHSITDLPLDLHLMVQNPDVAVDYFKVMPGDYVSFHPDTIEDPASLLKKI
                     KDKGAVAGVAISPKISLDYVSQLIDNLDFVLIMSVEPGFAGQRMLPASVEKVAELAKM
                     RADND"
     gene            complement(9759..10514)
                     /locus_tag="AC04VQ_00095"
     CDS             complement(9759..10514)
                     /locus_tag="AC04VQ_00095"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HIO9552680.1"
                     /translation="MASHKKILIKFVPIIVIGIALIFISLFCSCINNNNQNNKSLFDV
                     EFHRGGRDARPENTLYAYTYAIEQGATTIEGDMQMTKDGVIVMSHNPMLNSDITKDSN
                     GNYVQKNAIDIRTSNYDEINKYNVGEMKKDTEYYQLHGKTQKTTEAKIPKLEELLQLV
                     NDSGNKNIMLNLEIKSYPDPATGKYHENNVNYEKFLNEFNRIIKDYDFEDRMILQSFD
                     WKPLVMMKEINKNVLTSALYSEDQDEEQGIGTL"
     gene            complement(10613..10689)
                     /locus_tag="AC04VQ_00100"
     tRNA            complement(10613..10689)
                     /locus_tag="AC04VQ_00100"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10653..10655),aa:Pro,seq:ggg)
     gene            complement(10734..11561)
                     /locus_tag="AC04VQ_00105"
     CDS             complement(10734..11561)
                     /locus_tag="AC04VQ_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIO9552681.1"
                     /translation="MTKRKNIDHIAIFAVALLFIGLISVTLMACNQSNGDKGFNTIKE
                     GTLKVGVEVNNYPVAYKNSNDKIVGVEINYLEALCKEAGFEIEYVPMGFDRLIEAIKN
                     HEVDISMNQISETEARRQIVDFCEPYSKNGASLYFREDSTYTLDSLQEKADASEVKLA
                     TQDGTYFPNFKSRFKGNFVNVSYPTVDEAIAAVQKGEVDCFIYDSNPIARIDMTKNQH
                     LKDIFLDIMPVYNIAIALNKDLNGVKDKLDPIIKRMNNDQYLERVQEYFYTLDNPKT"
     gene            complement(11599..12456)
                     /locus_tag="AC04VQ_00110"
     CDS             complement(11599..12456)
                     /locus_tag="AC04VQ_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIO9552682.1"
                     /translation="MSKIFETKHCIIFAVVMFLVGAIALTFVGCNQIGSEPNENNTLK
                     AGKLTVAVEENNYPAVYKNDNGKIVGVEINYLEKLCKEAGFEVEYKTMPFNQIIDAIN
                     KHDVDVSMNQISVTDGRKSKVDFSEPYNKNGVSLYVKEDSSYTVDSLKQKAVRDKLNV
                     ATLDGTYYENFKSTLEGTVNNLPYSYVEDCIASVQSGETDCFVFDSNPIALIDFTKNK
                     HLKGLFLNDMPEYDIAIALNKNLKGVNEKLYPVMLKMNNERYLQRVQEYFYTLDNPKA
                     WDGILPAGK"
     gene            complement(12561..12637)
                     /locus_tag="AC04VQ_00115"
     tRNA            complement(12561..12637)
                     /locus_tag="AC04VQ_00115"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12601..12603),aa:Arg,seq:acg)
     gene            12711..12854
                     /locus_tag="AC04VQ_00120"
     CDS             12711..12854
                     /locus_tag="AC04VQ_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552683.1"
                     /translation="MQDFVLITEDIKNIISNDFFIGLILLSGVYFTIRLVFPQFRFAK
                     QLK"
     gene            12921..14024
                     /locus_tag="AC04VQ_00125"
                     /pseudo
     CDS             12921..14024
                     /locus_tag="AC04VQ_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013406.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005416 - amino acid:monoatomic cation
                     symporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
     gene            complement(14168..14593)
                     /locus_tag="AC04VQ_00130"
     CDS             complement(14168..14593)
                     /locus_tag="AC04VQ_00130"
                     /EC_number="3.5.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004132 - dCMP deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006220 - pyrimidine nucleotide metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009972 - obsolete cytidine deamination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidylate deaminase"
                     /protein_id="HIO9552684.1"
                     /translation="MTIAHQVASRTTCIRRAVGAIVVNDKRILATGYNGVPSGLKHCE
                     ETGCLREQQGVPSGQRHEICRGLHAEQNAIIQAAKSGTNLDGSVLYCTTQPCVVCAKM
                     IINTGIKEVVYTNPYPDELAMSLLKEAGIKLRVLEDFKV"
     gene            complement(14636..15085)
                     /gene="rpiB"
                     /locus_tag="AC04VQ_00135"
     CDS             complement(14636..15085)
                     /gene="rpiB"
                     /locus_tag="AC04VQ_00135"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HIO9552685.1"
                     /translation="MKIAIGTDHGAIDYKSEMIKHLTDKGFDVDDCGTNSTESCDYPD
                     FAKIVAEKVASGDADFGILMCGTGIGMSLAANKVKGIRAAVVPNVDYAKLSKQHNNAN
                     IICLSGRFMSLDDCKACVDAYMNEEFAGDRHERRVNKIMEIENENFK"
     gene            complement(15244..16227)
                     /gene="galE"
                     /locus_tag="AC04VQ_00140"
     CDS             complement(15244..16227)
                     /gene="galE"
                     /locus_tag="AC04VQ_00140"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIO9552686.1"
                     /translation="MPNKNLNKAPSETTVLVTGGAGYIGSHTCVELLNKGYKVVIVDD
                     LSNSKKEAVRRIKEITGVDDVQLIFYENNLLEMDVIQDIFKENEIDAIMHFAGYKAVG
                     ESVEKPLEYYVNNLMGSFVLCDCARDAGVKNLIFSSSATVYGDPAEIPITENCPKGNP
                     TNPYGQTKSMQEQVFQDIYVGDNEWNIVLLRYFNPIGAHESGKIGEDPKGIPNNLVPY
                     VSQVAVGKLDKIRVWGDDYDTPDGTGVRDYIHVVDLAKGHVAALEWMAKKNSGVEVFN
                     LGTGKGSSVLEVIKAFEKACGHELAYEIKDRRPGDIATCYADATKAKKEMG"
     gene            complement(16235..16774)
                     /gene="rbr"
                     /locus_tag="AC04VQ_00145"
     CDS             complement(16235..16774)
                     /gene="rbr"
                     /locus_tag="AC04VQ_00145"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012798333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HIO9552687.1"
                     /translation="MANKYAGTQTEKNLQEAFAGESQARNKYTYFAGVAKKEGYEQIA
                     ALFLETAGNEQVHAKQWFKELDGIGDTVANLNAAADGENFEWTDMYDGFAKTAEEEGF
                     KELACKFRAVAEVEKRHEERYRALLKNVEAKEVFAKSEVKVWECRKCGHICVGTEAPQ
                     ICPICAHPQAYFQIEPKNY"
     gene            complement(16802..17176)
                     /locus_tag="AC04VQ_00150"
     CDS             complement(16802..17176)
                     /locus_tag="AC04VQ_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013979482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HIO9552688.1"
                     /translation="MEIKYLKCAKCGQIVSVIQDTGVPIICCGQPMEPIEAGVTDGAV
                     EKHVPVYENSDNMLRVNVGIEDHPMTEEHYIQWIACHTKFGYQRKNLKPGDVPHAVFS
                     LVDNDEVLAVYAYCNLHGLWKK"
     gene            complement(17265..17354)
                     /locus_tag="AC04VQ_00155"
     tRNA            complement(17265..17354)
                     /locus_tag="AC04VQ_00155"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17318..17320),aa:Leu,seq:gag)
     gene            complement(17362..17625)
                     /gene="secG"
                     /locus_tag="AC04VQ_00160"
     CDS             complement(17362..17625)
                     /gene="secG"
                     /locus_tag="AC04VQ_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006361393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIO9552689.1"
                     /translation="MEWYLIILLILLFLSGTGLIIFILLHSGKGTGVSDMIASSVYSS
                     QTGTSIVEKNLDRITIVLAVIFLLTLIALMILYPQGTIGGASS"
     gene            17748..17963
                     /locus_tag="AC04VQ_00165"
     CDS             17748..17963
                     /locus_tag="AC04VQ_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552690.1"
                     /translation="MCGICGFNNTENGDFSTLENMCNTMIHRGPDAFGYYINDGIAFG
                     HRRLSLVDLKHGGQPIIRKSAEKKAQS"
     gene            18000..18971
                     /gene="asnB"
                     /locus_tag="AC04VQ_00170"
                     /pseudo
     CDS             18000..18971
                     /gene="asnB"
                     /locus_tag="AC04VQ_00170"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012936.7,HMM:NF024927.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
     gene            19107..19439
                     /locus_tag="AC04VQ_00175"
     CDS             19107..19439
                     /locus_tag="AC04VQ_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HIO9552691.1"
                     /translation="MSERNQLLKTKSNNMKTPQEITKKTYAEANADGLDDCAKMQYTD
                     LNFWILGDIFMKGDKMAMAHCLECRVPFLDTHVFEVARKLPTNEKISSVQTKIALRDA
                     AKKVIPND"
     gene            complement(19670..21085)
                     /locus_tag="AC04VQ_00180"
     CDS             complement(19670..21085)
                     /locus_tag="AC04VQ_00180"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIO9552692.1"
                     /translation="MNDLCSKLSQDYKIIGNAEAEVTGLELNSKEVKPGYAFFCIVGF
                     EADGHKFADNAVENGATCLFVEHELENIDVPQVIVKNTRDVMAEVADIFYGCPTEKFN
                     VVGITGTNGKTTITNIISHVLTTCNRHCGLVGTNGIKIDGVETDSEDFKFVGRTTPEA
                     FQLQKIMYEFKQRGINNIVMEVSSHAIEQNRIKGIRFGVTAFTNLTQDHLDYHKTMEN
                     YFEAKAKLFSKEYPAKRVICIDDEWGQKLYKQAYMNGEDVISVGLNKDALFNLDKVVV
                     NFPLIGRFNQQNMLVAYGICRQLGFDHDEIVNALETMPVVEGRMQLVEVGEKKPKVFV
                     DYCHTPDALQKAIETLKNDCNGKVFVVVGCGGDRDKDKRPKMGKIAVESSDYAIITSD
                     NPRREKPEDIIDDIVAGIKAYKNYEVEVDRASAIEKAIKMAEAQDFVLLAGKGHEKYQ
                     EIGTKKHHFDDVEVALSALNN"
     gene            complement(21201..22685)
                     /locus_tag="AC04VQ_00185"
                     /pseudo
     CDS             complement(21201..22685)
                     /locus_tag="AC04VQ_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013406.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            complement(22910..23110)
                     /locus_tag="AC04VQ_00190"
     CDS             complement(22910..23110)
                     /locus_tag="AC04VQ_00190"
                     /inference="COORDINATES: protein motif:HMM:NF036996.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552693.1"
                     /translation="MDNTKKPVVISSRTSSGTISEESLLSKNSIATQFLNPQKAAVLL
                     RVALINNLKRSNEDIERIFRTY"
     gene            complement(23120..23890)
                     /locus_tag="AC04VQ_00195"
     CDS             complement(23120..23890)
                     /locus_tag="AC04VQ_00195"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012913.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIO9552694.1"
                     /translation="MGKKTVMILATGGTIAGSGKAGRGVGYKVATLTAESLIESVPQI
                     QDLANIKAEQLFNLGSDNITQDELLQLANRVNELEKDDSVDGIVITHGTDTMEENSYF
                     LNLVCDVRKPVVFTGSMRPATAISADGPLNLYQSILVATHDDAVDKPVMCVMSDTILD
                     SRTFFKTSTFSVETMKSAGLGHLGIIRDQEVIFTSKGDKSIHTSKSEFRIDGVKELPK
                     VSVVYFHIDADVKNLEFALENSDGVVVAGAGEGSFSRF"
     gene            complement(23916..24203)
                     /locus_tag="AC04VQ_00200"
     CDS             complement(23916..24203)
                     /locus_tag="AC04VQ_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552695.1"
                     /translation="MKVCPTCKSRCFDDMDTCYGCMHHFSDSNYETENKNKEVTMNEK
                     IPEYFELDSFPFIEDEDYFRTRGAHERIMNNDEKIVIKLELPKSVLEKYIV"
     gene            complement(24622..24927)
                     /locus_tag="AC04VQ_00205"
     CDS             complement(24622..24927)
                     /locus_tag="AC04VQ_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552696.1"
                     /translation="MLCKSEKIKLLIGLLIVASIFVFIGISTYFEKAEDYDSLLNYDT
                     NYSSNIPENYLDEVVDVSSLDAHLIMIDKSEKVVGFSIDNDPVTSFNNLKDQLLSRG"
     gene            complement(24928..25308)
                     /locus_tag="AC04VQ_00210"
     CDS             complement(24928..25308)
                     /locus_tag="AC04VQ_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552697.1"
                     /translation="MFPVILIIAVICVNALTFFSECSSFDRTFKQIVTSLGPNPGYGK
                     DISNVKSLLESELQNRFDDDFLDFEVSVENVSGGLQEFSGNLKMYPTLFGMGLRSEIF
                     GVPLPSLNHQQKMTIEVYKPGVII"
     gene            complement(25422..25799)
                     /locus_tag="AC04VQ_00215"
     CDS             complement(25422..25799)
                     /locus_tag="AC04VQ_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552698.1"
                     /translation="MQDLGLEPANLNALKPVLINSTYVARAEENEDPTSTVGIASKTF
                     ASKFLVVKDTAISFTEANNSLLSAVSSGSELAAVLGLHDVIYSGGVIELATIEPLGNL
                     GPSIPITIKLPEFATGTMQNIVS"
     gene            complement(25932..26666)
                     /locus_tag="AC04VQ_00220"
     CDS             complement(25932..26666)
                     /locus_tag="AC04VQ_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552699.1"
                     /translation="MFYQYAVEQFSHAYVIDTGDTFQANFPHLPKNTDEMKQTSMYTN
                     VSFPISGGNCMHAYADCPGCSGISGYGTLAQMDGMGGFVQCPYCKFSASSLGSVASAS
                     TAISNGYEYHYKIVEEASKDYQKAKEEAEPLNRKVKNFADGIFDKIKKLISQIANKRI
                     YAKPPGSYGAICMTVNTNSAPASKGFESSFVSSETTLGVRAAISAATIIEEESDEGKT
                     VLNSILDGLTDELPTVSGAVGIALNA"
     gene            complement(26778..27533)
                     /locus_tag="AC04VQ_00225"
     CDS             complement(26778..27533)
                     /locus_tag="AC04VQ_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552700.1"
                     /translation="MIAINNIFKNEDGFTTLSVVVSLLLTLSLIFSTAQVYRISSASA
                     DIQNVSDSAVQAAENQVAEFMILVRVSDSVILSLSLGGIVCAALGVVAMCTPVTAAAS
                     DALLEASTKMFNARDSFADKSAKSLNQMQKALPFLASVKAFQVSQANNGGTFDANYIS
                     IAMLMPGEGITIEPGNINEAKGVLEEINANSDDIKKAAAEAEEAAKKANEAKVRAFMA
                     DCGNNPNYCMYERAKNKAYMTGPENPLYNSPDT"
     gene            complement(27581..28006)
                     /locus_tag="AC04VQ_00230"
     CDS             complement(27581..28006)
                     /locus_tag="AC04VQ_00230"
                     /inference="COORDINATES: protein motif:HMM:NF019428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HIO9552701.1"
                     /translation="MKGQATVEAAIMIPAIFLLLLLLIQPGIYLYDLCVMNEAASETC
                     RVLTTASDSEKSKICEPFARRRLSAIPQQDNFHVHSSGCSYEIEYVGSQGDNEVQVTI
                     KNQLKPLPLIGFLSDMFGLLNENNCFELKAESTQVSRPS"
     gene            complement(28449..28736)
                     /locus_tag="AC04VQ_00235"
     CDS             complement(28449..28736)
                     /locus_tag="AC04VQ_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIO9552702.1"
                     /translation="MCTREESLRNLAKSYDSQLLSRIIENIIRSLRFGSSLVEGLEEA
                     ALESRNLYKAKQEEIVSKAPVKMMIPTGTLILPAMLILVLGPILLELGSGF"
     gene            complement(28746..29087)
                     /locus_tag="AC04VQ_00240"
     CDS             complement(28746..29087)
                     /locus_tag="AC04VQ_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552703.1"
                     /translation="MPNVFGNFALREVQFRFAFIGFSSGALIGVVFSYILMVILGIIG
                     LIVGLYLPVWAMKQEKIIKNNNLDKCLPEMLEVVSLGLRSGLTFDRSFRIYTLHFNNE
                     FSKSCLTAQRK"
     gene            complement(29157..29408)
                     /locus_tag="AC04VQ_00245"
     CDS             complement(29157..29408)
                     /locus_tag="AC04VQ_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552704.1"
                     /translation="MSTYIYIAILIFAIAFAAICGVLIGRLILIRNKENLSRKFRREA
                     AGVEDVEFDNSFVCSGFARRIMNYLLKVSYSISHVNDQL"
     gene            complement(29409..30224)
                     /locus_tag="AC04VQ_00250"
     CDS             complement(29409..30224)
                     /locus_tag="AC04VQ_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIO9552705.1"
                     /translation="MRNGVSFLKKPSKFLLHHSKFIRTNFNNIVSLLNEKNLQCDEIS
                     VLSSSILLSFICGVVASLLFASFIAGIALILCIFVLIITISRSLIDKRITNLRNSIPD
                     TLRSMSTCFGAGYTIFQTFSHISSESKGSVKNLFGKCVHILQTGGSISSSLDCLKNVK
                     EVPELSFLAVALDVQHQTGGSMKPVIESAKDMVENKLELMRMLQVQTAQAKLSARIVM
                     VLPFALIAIFSMISPGFLTPFFSSIFGFILLLIACLMQTGGIILVKKMLNVEI"
     gene            complement(30458..31288)
                     /locus_tag="AC04VQ_00255"
                     /pseudo
     CDS             complement(30458..31288)
                     /locus_tag="AC04VQ_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012652.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
     gene            complement(31475..31711)
                     /locus_tag="AC04VQ_00260"
     CDS             complement(31475..31711)
                     /locus_tag="AC04VQ_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552706.1"
                     /translation="MSLYERAQKTSNPNLTKGQIATGNFTVKASPKSNLAILHKQVQE
                     IISPDKLAEYMTNNPQRAKSEIRNAANRCFMSSK"
     gene            complement(31712..32098)
                     /locus_tag="AC04VQ_00265"
     CDS             complement(31712..32098)
                     /locus_tag="AC04VQ_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552707.1"
                     /translation="MQATKKAMDLCNRCGIATGSVFFAINKCSKRALFSSIDISCALQ
                     GAPVAEIMDGGVEVDEFMSSGIPMELLQSRNSFAVSIWAILESIFPKELLENRQVDIN
                     KKKATRKSVKDKKKEKSGKLLRERRA"
     gene            complement(32171..32506)
                     /locus_tag="AC04VQ_00270"
                     /pseudo
     CDS             complement(32171..32506)
                     /locus_tag="AC04VQ_00270"
                     /inference="COORDINATES: protein motif:HMM:NF022077.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(32531..32803)
                     /locus_tag="AC04VQ_00275"
     CDS             complement(32531..32803)
                     /locus_tag="AC04VQ_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552708.1"
                     /translation="MCSNDDIEAINLAAAVKKDRPECVVCLISYDFSGSLQSRANAAG
                     IDTILSIESLCERISARKETSSISSADQTNEFKLRNIPQAIPQNNL"
     gene            complement(32980..33108)
                     /locus_tag="AC04VQ_00280"
     CDS             complement(32980..33108)
                     /locus_tag="AC04VQ_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552709.1"
                     /translation="MQELVAASQTTTLYFVLPSEEAEEIQSDKNKDEDKYFQNSNA"
     gene            complement(33136..33447)
                     /locus_tag="AC04VQ_00285"
     CDS             complement(33136..33447)
                     /locus_tag="AC04VQ_00285"
                     /inference="COORDINATES: protein motif:HMM:NF028286.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcpC/CpaB family pilus assembly protein"
                     /protein_id="HIO9552710.1"
                     /translation="MPDDAVRNSNDILGKQLTSSIVSGEVITSKRFQDSAVSLEVPAG
                     LSAVSVPAKDVSAVGGAINAGMHIDVYATGNISTSLIAQNVLVLATSTTFTEGTSASV
                     T"
     gene            complement(33460..33687)
                     /locus_tag="AC04VQ_00290"
     CDS             complement(33460..33687)
                     /locus_tag="AC04VQ_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552711.1"
                     /translation="MQLTKKRKSLVLCIVCGVVCALCVLIYTQSVRAEAEDTRNAALA
                     RYGGEQIEVCVAKRDIAAGEKIDSTCYELKL"
BASE COUNT        11286 a         6867 c         5114 g        10784 t
ORIGIN      
        1 agcaagcaag cgtcaaagta ataattgtga ttgacgcacc ttccatatta atagttgagc
       61 caattggaat tgttattgaa taagtctttt catctaagtg aagctcatca cagaatttca
      121 tatttacagg aatgtttgca gctgaagatc tcataaagaa agcagtaatt gcaacgtctt
      181 taatagttct aaaaataagc ggataagggt tccttcgcat aacaagtcca acaatcaaag
      241 cattaaatcc aaaagcaatg aatataagaa tcgataagta aacacacaaa agcaaaccat
      301 aatccgtaaa gattccaatt ccattatcca aaattgcagt gaagaccaaa cctgcaattc
      361 caaatggcgc aaaatcaata ataatcttta caattccggt tagaacttcg gcaatttttt
      421 gaaaaccttc tttaatgcgt tcaggcaaaa ttggtcgagc aataattcca aataaaattg
      481 accaaaaaat tacgccaaga aaattagcac ttgcaatcgc atctatagga ttagcaacaa
      541 tactcgtgaa aatatttaca acaatgtctc ccattccttc tggagcagaa tattgcgaaa
      601 tgtcaacttc agacggaagc gccaaagtca ctgggaaaag gacattaata acaacagcta
      661 taaaagcagc agaaaaagtt ccaattgcat aaaggcccat tgtcttttga acagtcttgc
      721 tgccagcctt ttttgcaccc attaaagatg atgtaattaa aaagaaaaca agaattggcg
      781 caatagctct aagcccacca ataaataaat tcccaaaaac ttgaaatgta accgcaagag
      841 ctccaagaca attaactaca atttcatttt catgcaatcc gccttgatga ttaacagcaa
      901 gattacacaa aacaccaaca gtagatccaa taatcaaacc aataactatg cgcaaaatga
      961 gattcgtatt acgatactta ataaatagtt ttttcattag tcaccaaact taatcttatt
     1021 tgttttttca tctatttcta aaattttaac taatgactca actctatacc cctcatcacg
     1081 aagttcattt ccgccatttt gataaacttt ttcaattgca attccaatgc cgtcaacttt
     1141 agcaccagct tgtatacaaa tagaaagtaa accacgaagc ggactgccct tcgctagaaa
     1201 atcatcaata attaaaatat ggtctttatt tgataagaat tttttcgaaa ctgtttcatc
     1261 atattctcga aaatgagtgt aggagataac ttttgaacta tatttttcac cattaatatt
     1321 tacactgtcc ttcttcttag cgaagacgag cggaatccca agtttttgag caacaggata
     1381 agcaacagca atacctgatg tctcaattgt tagaaccttc gtaaacttaa tgcccttaaa
     1441 acgtttaata aactcttcag ccattttatc cacgagttta gtatcgattt gatggtttaa
     1501 gaattcatca acttttagaa catcaccgtc tttgatttgt cctagctttt tgattttgtc
     1561 tttaagcaat ttcattgttt ctccaaaatg gtgggcccag caggattcga acctgcaacc
     1621 aaaggattat gagtcctctg ctccaccatt gagctatggg cccagcggta atgcggtatt
     1681 ttaccacaat ctattttatt atatgcagga taattgtggg cttacgcttt gctattttat
     1741 acaatttatt catgagttga cattcaatta ttaacaagtg gaatatattc atcatatgct
     1801 tcataataac aacgatgacg aactccttca aacatcactc acttagagtt cgcaatgcgt
     1861 ttatgcatat ctttcgcaat tgtttcggtg cataaatctt gagacccgtt tacaattaag
     1921 acattagtat taatttcttg caacttatca gaaaggtcaa tacaagaaac tgttccatca
     1981 agattatatt cgtcgatacc tcaagcagtt ttataaacat tatctcgacc tttagctttt
     2041 cctcgcctaa aacattctgg agcatcaaca acatttgtaa aatcatcaca agaatgtagc
     2101 aacataaatt tcttatcagc ttcaatgtag gattctaaat taaagttgtg tgttgcttcg
     2161 gcttcggcga ttgcattttg agaatcttta tccatctttg agattcactt acgctgttcg
     2221 tctgctcaca attttggatt acacaaacca gaagagatta ccatacttaa aacacctcga
     2281 gatttttttt caatgcaata agcaagctct aaaaatcctc caaaagattg cccaagcaaa
     2341 tgaatttgtt caagtctcaa aaactgacga agcgcttcta actcttcaat ccaagtatta
     2401 atatttcata attcagtgtg tccttcaaca taagattttc cacaacccaa ttgatcataa
     2461 taaattattt gacggtcttc tattaaagat aacacatcta aaacttcaag cgtattatgg
     2521 cttccaccag gtcctccgtg taaaactaaa aggggcgcct ttgcttcgtt gcctttacct
     2581 gcaattcgaa aataagtttt aaatccaaga taaggcatat aaccttcaat gactggcacg
     2641 agttttccat cggtatttca aaccacagaa gtactagttt cgcaatcttt aatatctttt
     2701 ggagcaataa cttgatacat tataaatctc aattcggaat aaaactttca ctacatgtta
     2761 agccgttctg ccaataataa tcttcgattc ctaaattatc tgcaaagttt agcacttctt
     2821 cgtaatcttc ttgagataca gtttttaata attcgggatg gtttttaaca acttcggatt
     2881 ctttatcaag cactggagta tattgattca taattgaaaa cttaattgag tttccaaatt
     2941 catcaaaaag catttttaaa acatcttttg aattttgtat gtgtccaggc aaaatcatat
     3001 gacgaacaat tacaccttta gtcattcgca aattctcttt aaaattgtca aacttaggtt
     3061 ttgaacaaaa acttgccatg gcatccaaac tttcaatcgc acaatcaaca taattagtaa
     3121 ctttattaaa agaaagctcc gaagctaagt cattgcttgc atatttaaaa tcagttaatc
     3181 aaatatccac agtatcattt aaagcataaa cactagacag cgtttcatag ccagaagtgt
     3241 tccaaacaat tggaagacaa agaccttttt gctttgcaag gcgtaccgca gaaataagat
     3301 gacttctata atgagtggct gtaactaggt taatgttcat tgctccctga ttttgaaggt
     3361 ctagcatcat atctacaagc tcatcaatgt ttgtctcttt accacaaaca tttccaacac
     3421 cagaaatctc acgattctga caataaacac atttcatgtt gcaattgctg aaaaatatag
     3481 tgccactacc agggccttga tcgcgtttcg gaccgcctat aattccgcta attggtggct
     3541 cttctcaata gtgaagtgca cttcgagcaa catataactt gtcatttgca ccacaaaggc
     3601 cagattcacc cgaagaccta ttcgctccac aagcaagtgg acatcaatcg catttatccg
     3661 gaactaaatt ttgccaagaa ttcgtcatta taattttgca caaggtggtt cgtaatgacg
     3721 tttatactcg cttcgtttta cctttgttaa aacagattga acatcaactt cgctgtaacc
     3781 taaaaataca atgtcttgag catttttgtt gttgtcaaca tgctcgcgta aaattgcatc
     3841 aagcgttgca tagtcaacgc caagacttgc ttcgtcagtt tgattatctg taagctccgc
     3901 acttggcgcg cgagtaataa cattttgagg aattacatct tcgttatttt caaacattct
     3961 acgctcttgt aaatttttag caatgcacat tttataaaca tcacatttat aaacatcacc
     4021 aattggcgca aatcctccgg ccatatctcc ataaagggtc gaatagccag ttcaaacctc
     4081 gcttttatta gcagtgttga gcatcaacca attattctcg tttgaagcag ccatcaaaac
     4141 tatcattctg catcgagctt gaatattttg cgaagttaaa tccgagattt gctcgtcttc
     4201 ggaatttaaa gaattaacaa aagcttcata tggcgaatta ataaaaacag tttttacctc
     4261 aatacctaaa tttgcggcta acttatttgc atcattaatt gaatcttgcg aagtgaacgg
     4321 tcccggcaaa atcattccat gaacatgttc acgtccaaaa gcatcaacgg caatttttgc
     4381 aacaagcgat gaatcaattc caccagaaag tcctataaca acatcactga atccactatc
     4441 agttacaaat ttttttgttt gctcgacgca agcgttatac attctcaaaa tttgatttgg
     4501 ttcatcacca gaaaagcatt cagtttcaaa attttcaagc tgttcttgta atggaataat
     4561 ttttggcata attcacccct aatcttcctg ttcaacttct attttgtcaa tatagacatc
     4621 gcgtccttcg ctaagttgag tctttattat tgcttccttg caaagtttat aatcatccaa
     4681 ttctttaagt gcgttaaaag cttcttcaaa catctcgtca ataatcgctg ctttttttcc
     4741 aacaacaaca tcaatctgtt taacacgcaa acaattttgt tctttcgcaa cactaacaac
     4801 agaatctaaa acaccggttg ctattccaag ttcatgcatt agtcgaacct ttaatcctca
     4861 ccaatcagtt cgcgttcttc ttctttttct tttttaatct ttcttgctaa aacaattctt
     4921 gagaacaaca aactaatttc atataacaaa actaaagcaa taaacaacaa aatcatagtt
     4981 actggcgaag catccggagt aataattgct gagaaaacta acagcacaac ataaataatt
     5041 cgccatgaac cacgaagctt tttataagga ataattccaa atactacaag atagaatact
     5101 atgagaggaa gttgaaacgc tattccaaat ccaatttcaa atttaataat tatgtctata
     5161 tatgccgaca tttgcggaat tacgtcccct agtccctgcg attgttccac aagtcactgg
     5221 attgctgttt ttaaaattac cacatagcaa aacacaacac ccaatataaa taaaactaca
     5281 gcagcaataa aagttggaat aaatcatctt cgctcttttg gtttaagcgc tggcaagaaa
     5341 aatgcaagca gttgccataa tatcatcggc gaagatgcaa ccaccgaaga ccagaacgca
     5401 attttaaatc ttgttccaaa tgattcgaat ggagtaagtg caaaaagttg cattccgttt
     5461 tcgtcttgtg gcaaaaaatc tttaattggc aaaagtaaaa attgtccaat ggttggtgct
     5521 gccgcataaa atactaaaat gcaaactacc aaagtgacga ggatgcgaac aattcgcata
     5581 cgaagttctc caaggtgatc catcaatggc attcttgctg gtccgatagg cattatttat
     5641 cacctttctt ttcagcagtt gcgttagttt gagatttatt atttttagat tcagatttaa
     5701 tatctttagg cttcggttta tccttcgggg gatcaggttc gtttgcaact ttcattacag
     5761 ggtcaacaag ttcttcttga acaaccttgt ccatttcggc tttagcttta cgaaatttct
     5821 taatagccaa accagcatac ctcgtaactt ctggcatttt ttttggacca aaaattacaa
     5881 agataaacac aactatgata aatagttcaa aaccacctat accaaacatc tgtgcctacc
     5941 tttaaataat aatttacgct gcttctgctt cagaattttg agcgctttgc tgattcattt
     6001 gactacaaac agttaatgaa actgtaggaa tacccaagcc taaaactaga attacacaaa
     6061 aaaatgtggt aataattttc tttctacgct ctcctcgagc ttgagctctt ttggcagctt
     6121 cttcactttt ctttgctgca tcaattttcg cctgcttttg cttaggcgtc attgttcttc
     6181 ttttactcat ttaataacct caatctttca tttagcaaaa atgggaggaa tatattacca
     6241 cataatagcc caaaaataaa acttttattg tttgttagtt cccaagaata aaacgttgaa
     6301 tatcttgatt cataacaaca acaaacaata gaagaattaa agcaattcca ataaaagaaa
     6361 taatattaat tgcacgaact gaaacattct tttttgttat aacttgatag atttctataa
     6421 gaaaacgacc gccatcaagt ggaggtattg gaagtaaatt cataatacca agtgaaatag
     6481 aaatcatcgc cataaattca aggaaggcac aaattcctgc ttcggcagca gattttgaaa
     6541 taacagcaat gccaatcaac gaagttgagt tcgaaacagt ttcagccatt gttgctgggt
     6601 tgaataaatt aacaactgca caaattacag cccacagata atttatacca tattgaatag
     6661 cctcaatcgg tgtcatgttt atatgtgtaa tttctccggt agattgctgt tgaacgtcaa
     6721 aacctacaaa agaatacaaa acaacaaaca ctataaacgc aaataaaata tttacaaaaa
     6781 tacctgctaa cagaataata attcgctttc aaaaaggaag ggatcgatac tgcttaaaat
     6841 attcctcttt aaaatactta tctttatcct taacttcacg cttctcacct aatttatatc
     6901 catttatgtc agcagcaaaa tattcaattg gcttatcttt ataatctttc gattttccac
     6961 gaacaacaca acctcaatca accaattcat ccaaagcatc ttcggcttcg tcttttgaaa
     7021 tttttaattc atgagcaact aaaggaattg tagttttccc gcttttatga ataaactcca
     7081 aagtttcagc caaaaatggc tgcatatttc ctggttccat gccacaaact cttgcataac
     7141 cgccaagcgg aacacaagta actccataac gcgtatcgtt gtgcttaaaa cttaaacgag
     7201 gaccaggaag acccaacata aactcgctaa cacgaacacc aaataaacgt gcagtaacga
     7261 agtgcccacc ttcgtgaaga aaaacgagca cgctaagaac aattaaacaa cacaatatag
     7321 ttattaaaat atccatcggg cgataaattc taccacaaaa aattaattag aaattatact
     7381 tgacaagaat aaaataaatg gtaatgtggt cgcctgtact taaggaaagg agaaggtatg
     7441 ccaagaccta ttatcaacac agaagattgt acagcttgtg gttcttgcgt tgaagtttgc
     7501 cctaatggtg tacttgaaat cgaagacact gccgtttctg tagtcaacga agacgaatgc
     7561 acagcatgtg aatcttgcat ggaagaatgc ccaatgggtg cgattaccaa aatcgaagaa
     7621 gattaatttc tttaaacata aattatgaaa agcagggttt cggccctgtt tttttataca
     7681 ttaattaaat tttgaatttt tgcaactgcc aaagcaatgt cttgaacttt ttgataattc
     7741 ttttgaatat ttcttttcgt taaatggttt aacaaatttg aattaagaac gaataaatgc
     7801 atccaaccat ttaaaaagaa aataattact ctttttccag ttacttcaat tagtttgtta
     7861 atagcttcaa ttacttgagg agtcaaaatg taaaataccg tttcagcatc atcacaaaaa
     7921 atatctaagt tgtcgttaag cttgatatct tctgtttcga taaattcatc aacattatag
     7981 attacagatg atgtgtaggt caaaaaaagc ggattattaa ttttcttctt aatcttaata
     8041 gaataagaat ccgaaaacaa aagtgcatga caatagttgt aatagatctt attattttta
     8101 ttaattgtaa tttcaacagg gttttctaac tgctctgtat aattatccca tgtttcttca
     8161 tcgcctcttt ttggtatatc aatataatta agaaaatctt tatatttata aactaccttg
     8221 cttttcttca tattagatgg aagattctca agagcttcgc ttcttttagt tttttttgct
     8281 atagatcttt tttgatgcct tgaaataaga atgatagcgc caacaagaat aaatacataa
     8341 ataactaaag ctcaaatact tgatgaatct ctatcgttaa aagggagcaa aacaataaat
     8401 aaaaaaggcg atcaaagcaa agcggcaagg attcaattaa ttacgtgtcg cttgaatgaa
     8461 gcgtttcctt cgttgtaatt cattcttcat aaagtaacag gacacaatgt agcacctata
     8521 aaacctatca taaatgacat aaaaatagta cccggtaatt tcctcgattc ttcagcatca
     8581 gcaagcataa tatttgaaag aataaatgtg ataataaaat aaacagagaa cataacaatg
     8641 ccataaatga ttaattctcg tttagaacga atattaggtt tacggttacc tcgtttattg
     8701 atataagaat cagcggatga catcttaata tctccaaact agttattaaa ctcttctaga
     8761 tatttatcaa gagttgttgc aaaatcttct gctttgttat ttttatttat caatgccgaa
     8821 cctacaacac atccatcagc tccagcatca acaaccttac gagcgttctt cggagcacag
     8881 tctccatcaa caaaaatttc aatgtttcaa tcattgtcag ctctcatttt tgcaagctct
     8941 gcaacttttt caacagaagc aggaagcata cgctgaccgg caaagccagg ttcaacactc
     9001 attattaaaa caaagtcaag attatcaatt aactgagata cataatcaag agaaatcttc
     9061 ggactaattg caacaccagc aaccgctccc ttgtctttaa ttttcttcaa caacgaagcc
     9121 gggtcttcga ttgtatcagg atgaaaactt acataatctc ctggcatcac tttaaaataa
     9181 tcaactgcaa catccggatt ttggaccatt aaatgcaggt caagaggcaa atctgtgatt
     9241 gaatgaatgt ctgcatatat cccagttcca acagaaatat taggaacata atttccatcc
     9301 ataacatcgt aatgaattaa cgggattccc gtttcttcga atttttctac ataagtttta
     9361 acatcccatg gcgcggaaca cataattgat ggtgctatct tcatactgac tcctttttta
     9421 ttagtttaat caagatgata tctactttca aactgaaact tttcagcaac tggtttattt
     9481 ttagttttaa taaaatctca taaaagctca cctttatcag aaattattcc atcaacaccc
     9541 attccaaaac atttttccat atcctcatta gtattaactg ttcaactaac tactttcata
     9601 tttcaattgt gggcttcatc aacttgccac ttttcaattt gatctcattg tggagaaata
     9661 atatcaatac caagttcgtg agcaacacgt acagcatcat cattataatc atggatattt
     9721 tttcctgcaa gtcatggaga tgcttgaggg acagttaatc acaatgtgcc aatcccttgt
     9781 tcttcatctt ggtcttcact ataaagagca gaagttaata catttttatt gatttctttc
     9841 atcataacaa gaggtttcca gtcaaaggat tgcagaatca ttctatcttc aaaatcataa
     9901 tcttttatga ttctattaaa ttcatttaaa aacttttcat agttaacatt attttcatgg
     9961 tatttcccag tagccgggtc tggataagat ttaatttcaa gattcaacat aatattttta
    10021 tttccagaat cgtttactaa ttgtaataat tcttcaagtt ttggaatttt ggcttctgta
    10081 gttttttgag ttttaccatg aagttgatag tattcggtat ctttcttcat ctcacctaca
    10141 ttgtatttat taatctcgtc gtaattgctt gttctaatgt ctatagcatt cttctgtaca
    10201 taatttccat tagaatcttt agtaatatcg ctattaagca taggattatg gctcataaca
    10261 ataacaccat cttttgtcat ttgcatatca ccttcaatag tagtagcgcc ttgctctatt
    10321 gcatatgtat acgcatacaa agtattttct ggtctagcgt ctcttccacc acgatgaaac
    10381 tcaacatcaa ataatgattt attattttga ttattgttat ttatgcaaga acaaaataga
    10441 gaaataaaaa ttagtgcaat tccaataaca attataggaa caaatttgat aagaattttt
    10501 ttatgcgaag ccataatcat cctttcaagt tattaacaaa tatagaaaac acaaaatatt
    10561 gtatcacttc tttaaaaatc taaatgattt tttaattcga taaataatga cgtggtcggg
    10621 tagactggac ttgaaccagc gaccccacga cccccagtcg tgtgcgctac caaactgcgc
    10681 cactacccga catatttact atgtaattat ttaccttgcg gcaaagtacc atctcaagtt
    10741 ttcggattat ctaacgtata aaaatattcc tgaactctct caagatattg atcgttgttc
    10801 atccttttta taattggatc caatttatct ttaacgccat ttaaatcttt attaagagca
    10861 atagcaatat tatatacagg cattatgtca agaaaaatat ccttaagatg ttggttcttt
    10921 gtcatatcaa ttcgagcaat agggttagaa tcataaataa aacaatctac ttcacctttt
    10981 tgcacagctg caatagcttc atcaacggta ggataagaca cattaacaaa atttccttta
    11041 aaccttgatt taaaattcgg aaaatacgta ccgtcttgag tagcaagctt aacttcagaa
    11101 gcgtcagctt tttcttgaag cgaatctaat gtgtaagttg aatcttctct aaaatataac
    11161 gaagcgccat tcttactgta aggttcacaa aaatctacaa tttgtctgcg agcttcagtc
    11221 tcgctaatct gattcatcga aatatcaact tcatgatttt taatagcttc aattaacctg
    11281 tcaaagccca ttggaacata ttcaatttca aaaccggcct ctttacataa agcttcaaga
    11341 tagttaattt caactccaac aattttgtca ttactatttt tataggcaac aggatagttg
    11401 tttacttcta caccaacctt taaagtgcct tcctttatag tattaaatcc cttatctcca
    11461 ttactttgat tacatgccat aagtgtcaca gaaattaaac caataaaaag caaagcaaca
    11521 gcaaaaattg caatatgatc tatattcttt cttttagtca tgattcctgg ccttttagtt
    11581 aataaagtaa atgccaaatt atttgcccgc tggtaaaata ccgtcccaag ccttaggatt
    11641 atctagagta taaaaatatt cctgtactct ttgtaaataa cgctcattat tcattttcaa
    11701 cataacagga tataattttt cattaacacc ctttaaattt ttattaaggg caatagcgat
    11761 atcatattca ggcatatcgt taaggaacaa tccctttaaa tgcttattct ttgtaaaatc
    11821 aatcaaggca attggattag aatcaaaaac aaaacagtca gtttcgccac tttgaactga
    11881 agcaatacaa tcttctacat atgagtaagg taaattattg acagttcctt ctagtgttga
    11941 tttaaagttc tcataatatg taccatccaa agttgcaaca ttaagtttat ctctaacagc
    12001 tttttgtttt aatgaatcaa ctgtataact agaatcttct ttaacatata aagaaacacc
    12061 attcttattg taaggttctg aaaaatcaac ttttgatttt cgaccatctg taacactaat
    12121 ctggttcata gaaacatcaa catcatgctt attaattgca tcgataattt gattgaatgg
    12181 catagtttta tactctactt caaaaccagc ttccttacat aatttttcaa gataattaat
    12241 ttctacacca acaatttttc cattatcatt tttataaaca gcaggataat tattctcttc
    12301 aacagcaaca gttagcttac cagcttttaa agtattattt tcattaggct cagaaccaat
    12361 ttgattacag ccaacaaaag ttaaagcaat ggccccaacc aagaacataa caacagcaaa
    12421 aataatacaa tgtttagttt caaaaatttt actcatgatt tcatcctttc aggtaaaaat
    12481 ttacaacgac aaaatatatc ataaaataat tacattagcc atataaaaat attttgttta
    12541 tggttgctgt gagataaaag tggcgcgcct tgagggattc gaacccccga cgtaccggtt
    12601 cgtagccggt ccctctatcc agctgaggta aaggcgcgcg tgagtgagta ttttgataca
    12661 tatatgcaca aatgtcaagg aaaatgctaa aatataggtg aaggtgatct atgcaagact
    12721 ttgttttaat tacagaagat ataaaaaata ttatctcaaa tgattttttc attggattaa
    12781 ttttacttag cggtgtttac tttacaatta gattagtttt tccacaattc agatttgcga
    12841 aacaactaaa gtgagcactt agtgataatg ctggaagtaa ggacgatgga atctcgccat
    12901 ttcaagcttt tatcacagct ttgggagctc gtgttggtgt tggaaatgtt gctggcatag
    12961 cttcggcgat tgcaactggt gggcctggag ctattttttg gatttgaatt tttggaattt
    13021 ttggttctgc aatttcattt ttagaaggcg ttttaggaca agcttacaaa ttaaaagacg
    13081 gtgacgaatt tttaggaggc gccgcttgat atatatggcg cggttttaaa aacaaaaagc
    13141 tttccaaagc aattggtctt ctttttgcag taattgcagt agtaagtttt ggaattttaa
    13201 tgcccggcgt tcaaagcaac actgttgtta acagcatcca tcaaggattc ggtgcagaca
    13261 aaacaataac gattgtaatt gcagcagttt ttattgcact aatcatatga ggcggcatca
    13321 aaagcattgg taaaattgaa gcaattttaa cacctataaa atgtgttgca tatcttgcat
    13381 ttgcagctat cgtcatatgc tattacggag aatatcttgg agaaacattt gaagcaatta
    13441 taacaagtgc tcttggaatt aatgcagtat tcggtggaat tgttggacaa gcaattacga
    13501 tgggtgtccg cagaggaatt tattctaccg atgttgctta cggacctggc ggaactttcg
    13561 cagctgcagc aagatgtgat catcctgtta aacaaggcat acttcaagca ctttctgttt
    13621 tactttcaac actattaata tgtacagcca cagctctcat ggttattcaa acgggtgctg
    13681 gacaaattgt tgctccaaac ggcgatattc tttatagcgg tccatcattt ccaggaactg
    13741 cagctggcgc cgaatgggtt caggcagctt taaaatctgt tccaatatta gaagcatgag
    13801 caccacaagt tttggctgtt atgattgcaa tttttgccgt aggaacaatc attggatttt
    13861 actatatcat tgagaacaat ctccgtttca ttatgggcaa gacaaataaa ggtgcaatct
    13921 tcattacaaa attggttttc ttggctgcat tagttataag cgcggttgtt gattcttcgt
    13981 ttatttggaa cactgctgac ataggcatgg gaatgcttgg ttgaataaat attatttgct
    14041 tattgttctt atcgccaaaa gcagttgcga ttgttaaaga ctttacagag gaaatcaagg
    14101 ctaaagaaaa acctaaattt gttcctgaga aatatggaat agatgacagt acaggtgcct
    14161 gaaaaaatta aaccttaaaa tcttctaaaa cacgaagttt aattccagct tcttttaaca
    14221 agctcattgc aagctcgtcc ggatatggat ttgtgtaaac gacttcctta attcctgtat
    14281 taataatcat ttttgcacaa acaacacatg gttgagtagt acaatataaa acagaaccat
    14341 ccaaatttgt accactctta gcagcctgaa tgatggcatt ttgctcagca tgaagaccac
    14401 gacaaatttc atgacgctgc ccacttggaa cgccttgttg ctcacgaaga caccccgtct
    14461 cctcacaatg cttcaatcca cttgggacac cgttgtagcc tgttgctaaa attcgcttgt
    14521 cattaacaac tatcgcgcca acagctctac gaatgcacgt tgtcctgctt gcaacttgat
    14581 gtgcaattgt cataaaatat tcatctcaac ttggtctatc agccacggca gcctcctact
    14641 taaagttttc gttttcaatt tccataattt tatttacgcg acgctcatgt ctgtcgccgg
    14701 caaattcttc gttcatataa gcatcgacac aagctttgca atcatcaaga ctcataaagc
    14761 gtcctgacaa acaaataata ttggcgttgt tatgttgctt cgaaagtttt gcataatcaa
    14821 catttggaac aacagcagcg cgaatcccct taaccttgtt tgcagcaaga gacattccta
    14881 taccagttcc acacattaaa attccgaagt cggcgtcacc actcgcaact ttttcagcaa
    14941 caatttttgc aaaatcagga tagtcgcaac tctcggttga atttgttccg caatcatcaa
    15001 catcaaaacc tttatcggtt agatgcttaa tcatttctga tttataatcg attgctccat
    15061 gatctgttcc aattgcaatt ttcactactc ttcctttcct ccaacacttt cttaacagga
    15121 cggagctttc gctacgctca acctaccgtc cctcgaccgt taagcttaac ggtaaccatc
    15181 cggattttgt ttttgccaat tccaagaatc ctcacacatt ttgtaaatgt caaattcagc
    15241 aactcatccc atttcttttt tggctttagt tgcgtctgca taacaagttg caatatcgcc
    15301 tggtcttcgg tctttaattt cataagcaag ttcatgaccg caagcctttt caaaagcttt
    15361 aataacttct aaaacacttg agccttttcc agttcccaaa ttaaaaacct caacgccact
    15421 attttttttg gccatccatt caagcgcggc aacatgaccc ttcgccaagt caactacatg
    15481 aatataatcg cgaacgccag tgccatccgg agtatcataa tcatcgcccc aaacgcgaat
    15541 cttgtccagc ttgccaacag caacttgcga aacataagga actaaattgt tcggaatgcc
    15601 cttcggatct tcgccgattt ttccagactc atgcgcgcca attggattga aataacgaag
    15661 caacacaatg ttccactcgt tatcgccaac atagatgtct tgaaagacct gttcttgcat
    15721 gctttttgtt tgaccataag ggttggtagg attgcccttt ggacaatttt cagtaattgg
    15781 aatttcagca ggatcgccat aaactgtagc gcttgatgaa aatattaaat ttttcacgcc
    15841 ggcatctctt gcacaatcac aaagcacaaa agatcccatt aaattattta cataatactc
    15901 aagcggtttt tcaacacttt caccaacagc tttataacct gcaaagtgca taattgcgtc
    15961 aatctcattt tctttgaaaa tatcttggat gacatccatt tcaagcaagt tattttcata
    16021 aaaaataagt tgcacatcat caacaccagt aatttcttta atgcgacgga ctgcttcctt
    16081 cttcgaattg ctcaagtcat cgacaatcac aactttataa cctttattta aaagttcaac
    16141 acaagtgtga ctgccaatat agccagcgcc accggtaact aaaacagtag tttccgatgg
    16201 cgctttgttt aagttcttat ttggcattta gctcttagta attctttggt tcaatctgaa
    16261 agtatgcttg tggatgagca caaattgggc aaatttgtgg agcttcggtt ccaacacaaa
    16321 tatgaccaca cttgcgacat tcccaaactt taacttcact ctttgcaaaa acttccttag
    16381 cttcaacatt tttaagaagt gcacgataac gttcttcatg acgcttctca acttcagcaa
    16441 cagcacggaa tttgcaagca agttccttaa agccctcttc ttcagcagtc tttgcgaaac
    16501 catcgtacat atctgtccat tcgaagttct caccatcagc tgcagcattt aaattagcta
    16561 cagtatcgcc aattccatca agctctttaa accattgctt tgcatgaacc tgctcatttc
    16621 cagcagtctc taaaaacaac gcagcaattt gctcataacc ttcttttttg gcaacgccag
    16681 caaaataagt gtatttgttg cgtgcctggc tctcacctgc gaaagcctct tgtaaattct
    16741 tttctgtttg tgtacctgca tatttattag ccattttttc ctcctttaac ttgttaatag
    16801 cttatttttt ccatagtccg tgcaaattac aatatgcata aactgcaaga acctcatcgt
    16861 tatctactaa tgaaaacact gcatgaggaa catcgcctgg ttttaaattc ttgcgttgat
    16921 aaccaaattt tgtatgacaa gcaatccatt gtatataatg ttcttctgtc attggatgat
    16981 cttcgatgcc aacattaact cgaagcatat tatcgctgtt ctcataaaca ggaacatgct
    17041 tttcaacagc accatcagta acgccagcct caattggctc cattggttga ccgcaacaaa
    17101 taattggaac tcctgtatct tgaataacag atacaatttg accgcatttc gcacatttta
    17161 aatacttaat ttccattatt atcatccttt catatataag atttatagca caattatttt
    17221 aacataaaaa agaaaaattc tcgggctaaa gccctcgaat tttttggtgt cggagatgag
    17281 atttgaactc acacgtccga taagtggaca caggcacctc aagcctgcgc gtctgccaat
    17341 tccgccactc cgactaatta actaagatga agctccacca atagtacctt gaggatatag
    17401 aatcatcaaa gcaattaagg tgagcaagaa aattactgca agaacaatag taattctgtc
    17461 aagatttttc tcaacaatac ttgttcctgt ctgacttgaa taaactgaac ttgcaatcat
    17521 gtccgaaaca ccagtacctt tgccagaatg taaaagaata aagataatca agcctgtgcc
    17581 agacaagaat aacaaaatca ataatattat taaataccac tccatgtaaa tctcctactt
    17641 acaaacttca cgtggactgc gaaattatac actttttatg gagttttgca aggttttttt
    17701 aatttagaca ggaatcgtgt ataattttac ccgcaaacgg aggatttatg tgtggaattt
    17761 gtggatttaa taatacagaa aatggcgatt ttagcacgct agaaaatatg tgtaatacta
    17821 tgattcatcg aggtccagat gcttttggtt attatattaa tgatggaatc gcatttgggc
    17881 atcgaagact ttctcttgtt gacctaaaac atggcggcca gcctataatt cgtaaatctg
    17941 ctgaaaaaaa agctcaatcc tgacatggaa caaaggaaag ttttgatgaa gtcggtgatt
    18001 tcgcgattgt tatgaatggc gaaatctata actataaaga aattcgatct gagcttgaaa
    18061 gcgacggtta tacatttaca acacaaagcg ataccgaagt tgtactaacc ggctatatta
    18121 aatgaggcga aaaaattctt aacaagcttc gtggaatgtt tgcaattgtt atttgggaca
    18181 aaaataataa agagctattt tgcgctcgtg atttttttgg tatcaaacct ttctattaca
    18241 caaaccaaaa aggtcagttt atttttgctt cggagattaa agcaattctt gagcatccaa
    18301 attatcaaaa agaactcaac accgaagcgc ttgagcaata cttatgtttt caattttctg
    18361 ctttgcaaga aactttcttt aaagatattt ttgttttgcc tcaaggacat tacatgaaag
    18421 ttaagaatgg aaaactttca ataaaacaat attggcgtcc aaaatttgaa caagataaaa
    18481 gtttgtctca tgagcaatgg gttaataaaa tcgacgaagc aatgcacgaa tctgttaaat
    18541 atcacaacat tgcagatgtc gaagttggtt ctctgctttc aagcggaatt gattcaagtt
    18601 ataacgccgc ccttttgcat gagattaacc caaaaattaa aacattcact gttggttttg
    18661 aggcttataa aaattcaggg tcagaaaagg gcgaacgcaa tgaaatagct tgagccaaag
    18721 agcttgcaga tagtaaaaat attgaaaact acagcaaaac aatctcagaa gaagaatact
    18781 ggaaagctct tccaaaaatt atgtgacaca tggacgaacc tcatggggat ccgagtgcag
    18841 ttgcacttta cttcgtagat caacttgctg ccaaaaatgt aaaagctgta ctttctggcg
    18901 aaggcgcaga cgaactcttc ggaggttaca caatttatca aacgtctctt tcttcaaaaa
    18961 agttaaattg acttccaaag tctgtaatta agggttcaaa aactttgatg tcaaaaatga
    19021 aaattcgcgg tgcaaagtat ttggacagag ttgaaaaagg tgtggaaaat tgatattata
    19081 caaatgcgta cgctagtgca tttaatgtgt ctgagagaaa tcaattgcta aaaacaaagt
    19141 ctaacaatat gaaaacacct caagaaatta caaaaaaaac ttatgcggaa gctaatgcag
    19201 atggtcttga tgattgtgca aaaatgcagt atacagacct aaatttctgg attcttgggg
    19261 acatttttat gaagggcgat aagatggcaa tggctcattg tcttgaatgt agagttcctt
    19321 tccttgacac tcacgttttt gaagttgcaa gaaaacttcc aaccaatgaa aaaatttctt
    19381 cggtacaaac caagattgca cttcgcgatg ctgccaaaaa agtaattcca aatgattgag
    19441 ctgaaaagaa aaagttgggc ttccctgttc cactttcaaa ttgacttcgt gaagataaat
    19501 attacaagca agttaaaaaa tattttacaa acaaaacttc aaataaattt ttcaacgaag
    19561 attatttgct tgacatttta gaagatcaca aaaaaggaat tgttgacaac tcaagaaaaa
    19621 tctgaatcgt ctacatgttt ttactctgac acgaaatctt ttttgagaat taattattta
    19681 gcgcacttaa cgcaacttca acatcatcga agtgatgttt tttagtacca atttcttggt
    19741 atttttcatg gcctttacca gctaaaagaa cgaagtcctg agcttcggcc attttaatag
    19801 ctttttcaat cgccgaagct ctgtcaacct cgacttcata atttttataa gctttaattc
    19861 cagcaacaat atcatcaata atgtcttcag gtttttcacg gcgcggatta tcactagtaa
    19921 taattgcata atccgaagat tcaacagcaa tcttacccat ttttggacgc ttgtctttat
    19981 ctctgtctcc tccacaacct acaacaacaa aaacttttcc attacaatca tttttcaaag
    20041 tttcaattgc tttttgcaag gcatcgggag tgtggcagta atcgacaaaa actttaggct
    20101 tcttttctcc aacttcaaca agttgcattc ttccttcaac aacaggcata gtttcaagcg
    20161 cattaacaat ttcatcatga tcaaaaccga gctgcctaca aattccataa gcaaccaaca
    20221 tattttgctg attaaatctt ccaataagag ggaaattaac aacaacttta tctaaattaa
    20281 acaatgcatc tttatttaaa ccaactgaaa ttacgtcttc gccgttcata taagcttgct
    20341 tataaagttt ttggccccat tcatcatcaa tacaaataac ccttttagct gggtattctt
    20401 tactgaaaag ctttgccttt gcctcgaagt aattttccat ggtcttatga taatcaagat
    20461 gatcttgagt taaatttgta aatgccgtta cgccgaatct aattccttta attctgtttt
    20521 gctcaatagc atgtgagcta acttccatta cgatattgtt aattcctcgt tgcttaaatt
    20581 catacataat tttttgaagc tgaaaagcct caggagttgt tcgaccaaca aatttaaaat
    20641 cttcactatc agtttcaaca ccatcaatct taattccgtt agttccaact aaaccacaat
    20701 gacgattaca agttgttaaa acgtgagaaa taatatttgt aattgttgtt tttccgttag
    20761 ttccagttat accaacaaca ttaaattttt cagttgggca tccgtaaaat atatcagcaa
    20821 cttcggccat aacatcgcga gtatttttaa caataacttg tgggacatca atattttcaa
    20881 gttcatgctc tacaaacaaa cacgttgcac cattttccac tgcgttatca gcgaatttat
    20941 gaccatcggc ttcaaatcca acaatacaga aaaaagcata accaggttta acttctttgc
    21001 tattaagttc aagcccggtt acttcggctt cggcgtttcc aataatttta taatcttgcg
    21061 aaagctttga acacaagtcg ttcaatttcc atatgatttt tgaatcactc atgttaaata
    21121 ttcctaataa cctctttatg ctctttggta atttgatctc attctggcaa attcaaattt
    21181 gcatttcccg aatgataaga tcaatcatcg tttgcatgct ctattaaata tttcttcgcg
    21241 acagaatttt tctctaaaat tttcttagcc ttcttggtag ttttatcatc cattcttgca
    21301 agcttattgt ttgttgtttc atattgaagc attgaatagt ggaaattaaa gttttgaaca
    21361 taatgcctat aagctctaag aacgtactta agcccaatat atagaagcgg caagttgcaa
    21421 taaaccatca aaatatttgc catatcactc aaattccaaa gtgcgcttaa gtcaagaccg
    21481 gaaaaattaa caattacacc aaacgctgtt acaaacaaac acataattct aataaagaag
    21541 acccatgctt ttgcacgagt aattcgcttc gcacaaattt cagtgaatga cacaaatcct
    21601 acaagtgtag ttagtgcaaa aagactgaag caaacacaca ttattataaa tacaatctcc
    21661 gaaggaatgc cttcgcctcc acacaaagat gtacaagacg ccgtaaactt ttcaagctta
    21721 ccaagatcca ttcactcccc accatttgga ccaagccaaa gttgagacat aattacaacg
    21781 aaggcagttg gcgtgcaaat aacaatcgtg tcgaagaaaa cgcccatcga ttgaaccatg
    21841 ccttgttcgc atggatgctt agcattactt gctgcggcag gcattgttat tgtaccttga
    21901 ccagcttcgt tactcataag acctcgacgt ataccttgct gaagtgcaac tccaaaagct
    21961 cctccaaaaa ttgcgttcgg ctgaaatgct tcggtgacta caacgtagaa aaaccaagga
    22021 attctatcga agttaattgc aatcaatgtc aaaactgttc cgatgtaaac aactgccata
    22081 attggaacgc ttacatcggt gaacttcgta acaaacttta ctccaccaaa agctaaaaag
    22141 ccaacgaaaa caatcatgat aaaaaacata actcagcaag ttggttgttc tggagaagaa
    22201 gcgctcggat caaacaattc ggcaattgct aaaaatccag aaattgtgtt aaagccttga
    22261 gccggcaaac acaaaaatgc gtagacaata tacaaaatac ttagagcaac tccaactccc
    22321 actgcattct tgattaactt ttgtccgtag aaagccatgc cgccaatata ttcatctcca
    22381 tcgcgctgtt taaaaatttg tgcgagcgtg ctttcaacaa aagcagttgc catcgaaaac
    22441 agggcagaca cccacattca caaaattgcg ccaggcccac caacgctaat agcgccagta
    22501 acaccaagaa tattacctgg tccaacacgc attgccgaag acaataaaaa cgaagctaag
    22561 ggagaaatac ccttgcgttt ttttgtgccc gaaaacaaaa ttttaattgc atgtttaaaa
    22621 taacgaaatt gcaaaaatcc aaaacgaaaa gtaaaaaaaa ttgcagtacc taaaagtgca
    22681 atcattgcaa tactaaaact acccaaaata ggaatattcg catatcaatc taaattggtt
    22741 ggaaagtctc ataagaaatc ggaaataatt tgtaactttg cacaaacttc aattactgca
    22801 gcaaataatg catccataaa cttccttcgc ttatccttat ctctagcccc gctatattat
    22861 accctaccaa tttcagcaaa gaacaaaaga tgaaaattta ataatgctat tagtaagttc
    22921 taaagatgcg ttcgatgtct tcgttgcttc gtttcaaatt attaattaag gcaactcgaa
    22981 gcaaaacagc agctttttga ggatttaaaa attgtgtagc aatgctattt ttagaaagaa
    23041 gagattcctc agaaatggtt ccagacgaag ttcttgaact aataacaaca ggcttttttg
    23101 tattatcaat tgcttcgcgt caaaacctac tgaaacttcc ttcaccggca ccagcaacaa
    23161 caaccccatc tgaattttca agagcaaatt ctagattttt tacatcggca tcaatatgaa
    23221 aataaaccac gcttaccttt ggaagttcct taacgccatc aattctaaat tcactctttg
    23281 atgtatgtat agacttatca cctttacttg taaaaattac ttcttggtcg cgaataattc
    23341 caaggtgacc aagtcccgca cttttcattg tctcaactga aaatgtcgaa gttttgaaaa
    23401 acgttctcga atctaaaatc gtatcgctca taacacacat tacaggttta tcaacagcat
    23461 cgtcatgtgt tgcaactaaa attgattgat ataagtttag cggaccatct gcagaaattg
    23521 ctgttgcagg cctcatcgaa ccggtaaaaa ctacaggctt tctaacatcg caaacaagat
    23581 ttaggaaata agaattttcc tccatagtgt cggttccgtg agttataaca attccatcta
    23641 ccgaatcatc tttttccaac tcattaactc tgttagcaag ttgcaacaat tcatcttgtg
    23701 taatattgtc agatccaaga ttaaacaact gttcagcctt tatattagca aggtcctgaa
    23761 tttgaggaac agattcaatc aaactttccg cagttaaagt agcaacctta taaccaacac
    23821 ctctgccagc ttttccagaa ccagcaatag ttccgccagt tgccaatatc attactgttt
    23881 tcttacccat aatttctcct tcttgcgaag gcatattata caatatattt ttctaaaaca
    23941 gatttaggca attcaagttt tatgacaatc ttttcatcat tattcataat tctttcatga
    24001 gcgccacggg ttctaaaata atcttcatct tcgataaatg gaaaagaatc aagttcaaaa
    24061 tattccggaa ttttctcatt cattgttacc tccttatttt tattttcagt ttcataatta
    24121 gaatcactaa aatgatgcat acatccataa cacgtatcca tatcatcaaa acatctactt
    24181 ttacaagttg gacaaacttt catgtcaata acctcctata taattatctt atcccctaaa
    24241 gataagaatt ttgaattatt actactaaac ttttcagcta cataaaacga atttttacaa
    24301 atcaaaactc ttcatggctt taacccaaca aaaactctta taactgattt gttcttatca
    24361 ataaatgcaa catcaatttt ccttctaaag aaaaaagtgt gtatacaatt gcacggataa
    24421 atcaaacaca tcgattttcc atttcgaaaa tataggccac gaaatctctt aataatcgaa
    24481 ttgcaaatta tataattagt caactgtcaa gacgacactg gtctctcctc ccacatttac
    24541 gcaattaaca aatagtcatt tatagtttcc gttattttta taaaaacttg acgaagtcga
    24601 ggatccgctt tcaacataat ttcaacctcg agacaacaat tgatctttta aattattaaa
    24661 cgaagttaca ggatcattgt caatagaaaa acccacaacc ttttcgcttt tgtcaatcat
    24721 tattaaatgt gcatccaaag aagaaacatc aactacttca tctaaataat tctcaggaat
    24781 attacttgaa tagttcgtat catagttaag aagcgaatca taatcttcgg ctttttcaaa
    24841 atatgtgcta attcctataa aaacaaatat agatgcaaca atcaaaagac cgattaataa
    24901 tttaattttt tcacttttac aaagcattta aataattact cctggtttat aaacttcaat
    24961 tgtcattttt tgttgatggt ttaagcttgg gagaggaacg ccaaatattt cacttcgaag
    25021 tcccattcca aataatgttg gatacatttt tagatttccg ctaaactctt gcagcccacc
    25081 actaacattt tctacgctta cttcaaagtc aaggaaatca tcatcaaatc tattttgcaa
    25141 ttcgctttct aataaagatt taacatttga aatatctttt ccataacctg gattaggtcc
    25201 taaacttgtt acaatttgtt taaacgttct atcaaaactt gaacattccg aaaaaaaagt
    25261 taatgcgtta acgcaaatta cagcaatgat taatatcaca gggaacatta cggcaagctc
    25321 aacagttgac tggccttcgt tattttttca taaactggaa attatcttca tatttttccg
    25381 cctcacaaat ttcctaaaat gctttgtaga gttcccgcaa ttcaactaac aatattttgc
    25441 atagtgcctg tcgcgaattc tggaagtttg attgtaattg gaatgcttgg tcctaaattt
    25501 ccaagaggct caattgttgc caattctatt acacctccag aataaatgac atcatgaagt
    25561 ccaagaacag cagctaattc agacccactt gaaactgctg aaagcaaaga attattcgct
    25621 tctgtaaaac ttattgctgt atcttttaca acaagaaatt tcgaagcgaa ggtcttgctt
    25681 gctattccaa cagttgaggt tggatcttcg ttttcttcag ctctggcaac ataagtggaa
    25741 tttatcaaaa caggctttag agcatttaaa tttgctggct ccaaacctaa gtcttgcata
    25801 aattcgctta atcgcttcga agctcaagtc cccaaaccac ttgcactaac tacaggcaat
    25861 tgattcaacg aagattccaa tccatccata aaagcttctt gcccattact ataaacctcc
    25921 aaaattttgg atcaagcatt aagagcaatt ccgacagcac cagaaactgt aggcaattca
    25981 tcagttaaac catcaagaat tgaattaagg actgtctttc cttcgtccga ctcctcttca
    26041 ataattgttg cagctgatat tgccgcacgg acacctaatg ttgtctcact cgaaacaaaa
    26101 ctgctttcaa aaccttttga ggctggagca gaatttgtat taacagtcat acaaattgca
    26161 ccatatgatc caggaggttt tgcataaatt cttttattcg caatttgact tattaatttt
    26221 ttaatcttgt caaaaattcc atcagcaaaa tttttaactt ttctatttag aggttcagct
    26281 tcctcctttg ctttttgata atctttggat gcttcttcaa caattttata gtgatattca
    26341 taaccattac taattgcagt cgaagcagaa gcaacacttc ctaagcttga tgcactaaac
    26401 ttacaatatg ggcattggac aaatccaccc attccatcca tctgtgcaag agttccatat
    26461 ccactaatcc cagaacaccc cggacaatca gcataagcat gcatgcaatt tccacctgaa
    26521 atcggaaagc ttacattcgt gtacatcgat gtttgcttca tttcatcagt gtttttagga
    26581 agatgtggaa agtttgcttg gaaagtgtct cctgtatcta taacataagc atgcgaaaat
    26641 tgctcaacag catattggta aaacacacga cgaagtgcag aattcgcctt cgcatcaacc
    26701 gaaggtccat ctggtgcctc gatttgaatt ctttttggat aatatgcttg acaacgtttt
    26761 aagggtaccg aaaagcttca ggtatctgga ctattatata gagggttttc aggtcctgtc
    26821 atatatgcct tgttctttgc acgctcatac atacaataat ttggattatt tccgcaatca
    26881 gccataaacg cccgcacttt tgcttcgtta gctttttttg cagcctcttc tgcttccgcc
    26941 gctgcctttt ttatatcatc agaattagcg ttaatttctt caagaactcc ttttgcctca
    27001 ttgatatttc caggttcaat agtaattcct tcaccaggca ttaacatcgc gatagaaatg
    27061 tagttagcat caaaagttcc accattattt gcctgtgata cctgaaaagc tttaactgat
    27121 gctaaaaacg gaagagcttt ttgcatttga tttaaactct ttgcgctttt atcagcaaaa
    27181 gaatcacgag cgttaaacat ttttgttgaa gcttccaaaa gagcatccga agcagcagct
    27241 gtcactggtg tacacattgc aactactcca agagcggcgc aaacgatccc gcctaaactt
    27301 aaagacagta ttaccgaatc tgatacgcga accaaaatca taaattctgc tacttgattt
    27361 tccgctgcct gcacggcaga atcagacaca ttttgaatat ctgccgaagc actgctaatg
    27421 cgatacacct gtgcagttga aaagattaaa gaaagtgtaa gcaaaagaga tacaactaca
    27481 cttaaagttg tgaagccatc ctcgttttta aatatattat ttatggcaat catcccccaa
    27541 ctcaattttc aggatttttc ccttgtggac tattagttac tcacgaaggt cttgaaactt
    27601 gtgtcgattc tgcttttaac tcaaaacaat tattttcgtt taatagtcca aacatatcgc
    27661 ttaaaaatcc aattagcgga agaggcttta attgattctt gattgtaact tgaacttcat
    27721 tgtcaccttg acttccaacg tattcgattt catacgaaca acctgaacta tgaacatgaa
    27781 aattatcctg ttgaggaatt gcagacagcc ttcgacgtgc aaaaggttca caaattttcg
    27841 acttttcact atcactggct gtagtcaaaa ccctacaagt ctccgaagcg gcttcgttca
    27901 tcacacacaa atcatataaa taaataccag gctgaataag caacagcaac aacaagaata
    27961 tggccggaat catgattgcc gcttcaacag tagcctgacc ttttatatct tttcaaaaac
    28021 ttaacaacta aaaacatctc caactattcc tgcagcggcg tcttcaagat gatgcgaagc
    28081 tgaactttgt gcgtgatcaa caaaaattcc atcatctcca agatttcaaa ataaaccaag
    28141 agcaattaca actacaagca ttgcagcagt gacaattgca aactcaacag tgctttgacc
    28201 actctcggaa tttattttta aaagaatttt tttcataact tacaaactat taattccttc
    28261 agcaattgaa tttcacaatt cttcaagctt cggtttaaaa atagcaatcg caattattgc
    28321 aatcacaacc aagacgccaa ctaaaattgc atattcagtt gttccttgac cgctttcgtc
    28381 tcaaagtttg acttgaaaat ttgaaactct cttaatgctt caatgtttaa ttttcatttt
    28441 tgctcctttt aaaatccgct tccaagttcc aataaaattg gacctaatac taaaatcaac
    28501 atagcaggca aaattaaagt ccctgttgga atcatcattt ttacaggagc tttagataca
    28561 atttcttcct gctttgcttt atataaattt cgtgattcaa gtgcagcttc ttccaatccc
    28621 tcaacaagag atgaaccaaa acgcaaagat ctaataatgt tttcaataat tctcgaaagc
    28681 aattggctgt cataactttt tgcaaggttc cgaagcgatt cttcgcgagt gcaaattccg
    28741 ccttctcatt ttcgttgagc cgttaaacaa gatttagaaa attcgttatt gaaatgtaag
    28801 gtataaattc taaatgagcg gtcaaatgtt aaaccacttc gaagaccaag cgaaacaacc
    28861 tccaacattt caggcaaaca tttatcaaga ttgttatttt taattatttt ttcttgtttc
    28921 attgcccaaa caggcaaata taaacccacg attaatccaa taattccaag aattaccatt
    28981 aatatatacg aaaaaacaac cccaattagt gctccagaac taaagccaat aaaagcaaat
    29041 cgaaattgaa cttctctaag agcaaaatta ccaaatacat ttggcataga acacatggtc
    29101 ttaagttttt tgaatttttt tggtaattca tcatttttaa taaacttttt tgttattcat
    29161 aactgatcat ttacatggct tatagaatat gaaactttta atagatagtt cataattctt
    29221 cttgcaaatc cactacaaac aaatgaatta tcaaactcta catcctctac cccagcagct
    29281 tcccttcgaa atttcctcga taaattttct ttgtttcgaa ttaagattaa ccgtccaatt
    29341 aaaacaccac aaatagcagc aaacgctatt gcaaaaatta aaattgctat gtaaatatac
    29401 gtactcattt aaatttcaac atttaacatt ttcttaacca aaattattcc gcctgtttgc
    29461 attaaacaag caattagcaa taaaataaaa ccaaaaatgc ttgaaaaaaa tggagtcaaa
    29521 aaacctggac ttatcattga aaatatcgcg atcaaagcaa acggtaaaac catcacaatc
    29581 cgggctgata attttgcttg tgcagtttga acttgaagca tcctcataag ctcaagtttg
    29641 ttctctacca tatctttagc cgactcaatc actggcttca tgcttccacc cgtttgatgt
    29701 tgaacatcta aagcaactgc caaaaatgaa agctcgggaa cttccttaac attttttaaa
    29761 caatcaagcg aacttgaaat tgaccctcca gtttgcaaaa tatgaacaca cttgccaaat
    29821 aaatttttta cagatccctt gctttcagaa ctaatatgag aaaaggtttg aaaaattgta
    29881 taaccagcac caaaacaagt actcattgac cttaaagtgt ctggaattga attgcgcaag
    29941 tttgtaattc gcttatcaat taaagatctg cttatagtaa ttattaaaac aaagatacac
    30001 aaaattaaag caatgccagc aataaatgat gcaaataata acgaagcaac aacaccacat
    30061 atgaaactta ataatatgct tgaagataaa acagagattt catcgcattg aagatttttt
    30121 tcatttaaca aagaaacgat gttattgaaa ttagtcctaa taaactttga atgatgtagc
    30181 aaaaacttcg aaggcttttt taaaaaagaa actccatttc tcattaacca tgcaaagtaa
    30241 ttattattgt aatcattcga agaatcaatc ccatagttcg aaagccttcg ctttttatat
    30301 cattcaataa tgcgcttaaa tataaagaag ccacaaatgc aagcacagat aattgcaaat
    30361 aatattaagc agatgcgcaa gattttcata aacttacctc ctgctttgtt gaaaacttgt
    30421 ataacggaac atcgtcaatc cagtttggga aacttaatca ttttcctgat ctatcaaaag
    30481 gcccacgata aaataactgc ttaacttcac attttttatt ttcttcatta aaactaaaag
    30541 caacaacatc actaatatat cttgaaccgt caacacctct tgatgtttga acaactaaat
    30601 caatcgctga agcaatataa ttttcaataa catcaactgg aagttctgca gcataacgaa
    30661 ccattgttgt taatcttgaa attgtttctt ttggtgaatt tgcatgaagt gtagtaagag
    30721 aaccatcatg tcctgtattc attgcttgaa gcatatcaag agcctcggca cctctacatt
    30781 ctccaacaac aattctgtct gggcgcattc gaagtgcatt aataactaaa tcgcgaattg
    30841 taacttcgcc agttccttcg gcattccgag gccttgcttc caacctaatt acatgaggat
    30901 gctctaaaaa acgaagttct gcagagtcct ctattgtaat aactctttcc ctgtgagaaa
    30961 ttttacaaga caacgcattt aacaaggttg ttttgccgct tcctgttcct ccagaaacag
    31021 caatattttt tcttattaga acagcccacg ttaaaaattt aaggagcttt tcgtctaaag
    31081 aattcattgt agccatttct tctaaagtga ttacatgttc agcaaatttt ctaatagtta
    31141 aaatggttcc atctagagat aatggcgaaa ttattgcatg aaccctatga cctgaactta
    31201 gcctggcgtt aaccataggt gattcttcgt caattcttcg tcctaatgga cctaaaatac
    31261 gatctactaa agaacgtact tgctcatcat tattaaaatg ttgttttcaa cgatgcagct
    31321 tcccttcaag ttcataataa attgaattcg ttccatttac cattacttca gtaatttttg
    31381 gattatcaag tagcacctcg ataggcccaa acccaaacat aaaatctatc aaattcttta
    31441 caagttcctt cttttcgtca tcatttaagg attctcattt tgaactcata aaacatcgat
    31501 tcgcagcatt gcgaatttct gattttgccc tttgaggatt attagtcata tattcagcca
    31561 atttgtcagg cgaaataatt tcttgaacct gcttatgtaa aattgctaaa ttacttttcg
    31621 ggcttgcttt tacagtaaaa ttaccagtag caatttgacc tttggtaaga ttaggatttg
    31681 atgtcttctg tgctcgttcg taaagtgaca tttatgctct acgctccctt aataactttc
    31741 ctgatttttc tttcttttta tctttaacgg attttcgtgt cgcttttttc ttattaatat
    31801 caacttgtct attttcaagt agttctttag ggaaaatact ttctaatatt gcccaaatac
    31861 ttacagcaaa tgaatttctt gattgaagca actccattgg aataccagaa gacataaact
    31921 catcaacttc gactcctcca tccataattt ctgcgacagg agcaccttga agtgcacatg
    31981 aaatatctat cgaagaaaac aatgctcgct ttgaacattt attaatggca aaaaatacag
    32041 accctgttgc tataccacac ctattacata ggtccatggc tttttttgta gcctgcaccg
    32101 atgttggtct ttgatcaata agaaaaactg acttgctatc tctttcaagc aacaaagctt
    32161 gttgttcgtt tcaataactg ccagtgttaa taacaattac atcgaaaatt tctttcagtg
    32221 attccaaaat tgcaggaaat tcctcgataa ccttttctga aatctcgggc aaagccgggg
    32281 cagaaattat gcacggtaag ttatttacag actttaattt tgaaacaata cctctgttgt
    32341 caattaattc atcaatccta attggtttat caattccaaa caatgtatca acatcaccaa
    32401 attgaaaatc tgcatcaatt attaatgttt taaaaccggc agcttggcac aaaagagatg
    32461 ccataacaga tacactgctt tttcctgatc ctccagaagc agaaagaact gaaagaacaa
    32521 aggcttttct tcataaatta ttttgcggaa tagcctgagg aatattacgt aacttaaatt
    32581 catttgtctg gtcagcacta ctaattgacg aagtttcttt acgagcactt attctttcgc
    32641 aaagagattc tattgataat atagtatcaa ttcctgcagc attcgctcga ctttgaagcg
    32701 atcctgaaaa atcataactt attaaacaca ctacacactc aggcctgtcc tttttaacgg
    32761 cagcggctaa attgattgct tcaatatcat catttgagca cactcaaatt tcaagactac
    32821 ttttgcataa gttaatataa tttcgaaatt tttgtgcatt agtaaatact tttagtcaat
    32881 catagttaaa tatgtttgaa tccaaattta aaaatccagg atttttacgg acttgagaat
    32941 caatgcttaa tacaacctct ttattcatta ttcctccgtt catgcatttg aattttgaaa
    33001 atatttatct tcgtctttat ttttgtcgga ctggatttct tcagcttctt cagaaggcaa
    33061 aacaaaatac aaagttgtcg tttgcgaggc tgctacaagc tcttgcacag cagaaggctc
    33121 aacagcaagt gttattcatg tcaccgaagc cgaagtccct tcggtgaaag ttgtgctcgt
    33181 tgctaaaact aatacatttt gggcaattaa acttgtagag atattacctg tagcataaac
    33241 atctatgtgc attccagcat taattgcacc accaacggca gaaacatctt tagctggaac
    33301 actaacagca gaaagacctg ctggaacctc caaagataca gccgaatctt gaaaccgttt
    33361 gctcgttatt acttcgccac taacaatcga gctcgttaat tgcttaccta aaatatcatt
    33421 tgaatttcga actgcatcat ctggcaaaag atcagcaagt cacaacttta attcataaca
    33481 cgtcgagtca atcttctctc cggctgcaat atctcgtttc gcaacacaaa cctcaatttg
    33541 ttcaccacca tatctagcca atgctgcatt tctggtgtct tcggcttcgg cacgaacaga
    33601 ttgagtataa ataagaacac ataaagcaca aacaacacca caaacaatac ataataccaa
    33661 cgattttctt ttttttgtta attgcatagc cttctcccaa cttgcttact ttgctaccca
    33721 gtacttgggg ctcgtaatat acacaaaaag gatcaaccag aaatgaaggc tcgcttaaaa
    33781 atctatgaag aatacttaaa catcacaata aaataataat taataataaa attgaataaa
    33841 aataaataat ttaaaaaata caaaaattaa aattactaaa taataatcaa cacacaataa
    33901 caaatactaa tatcccgctg cccccggccc cgaagaaaca aaaaataagg taaaataagc
    33961 gatattatgt tatagttgct gtttgcactt agaaaaagaa ggtgagaatt atgtcaaaag
    34021 tatgtgaaat atgtggaaaa gctccagctg c
//
LOCUS       DBNCDC010000002        32805 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_238_length_32805_cov_7.710260, whole genome shotgun sequence.
ACCESSION   DBNCDC010000002 DBNCDC010000000
VERSION     DBNCDC010000002.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 32805)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 32805)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32805
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_238_length_32805_cov_7.710260"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            175..381
                     /locus_tag="AC04VQ_00295"
     CDS             175..381
                     /locus_tag="AC04VQ_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552712.1"
                     /translation="MNALEPMQTVFHFIFKEAGGGGGGGGEDIEVVSAAAQTGDWLTI
                     LLVALFAIAAIASLAFVFMKNKQS"
     gene            547..1077
                     /locus_tag="AC04VQ_00300"
     CDS             547..1077
                     /locus_tag="AC04VQ_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552713.1"
                     /translation="MDYKAEIDVIVDEATQSISFDTNYFTNLSADPVHIDNSTLTLSD
                     EAKLLDIPEFVFKASYENDKANVFEGMSDETTPDIPTGQTVLATKATQNIKYSTNMTY
                     ETAKLLSTIENAFTLELNETPCYTIEYSANEATGGEAPAPVTLTPSAETGALNVETYA
                     PENTGGLVKTGYRFTG"
     gene            1453..2118
                     /locus_tag="AC04VQ_00305"
     CDS             1453..2118
                     /locus_tag="AC04VQ_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552714.1"
                     /translation="MPLSPNLTKDSKFTATSVESVVTITYKVVDENFPHGSISLNNSE
                     ENNDSEGFAAGVVTETFKPQLGETPKGATATPDTSTDYKYDFINWTKVGSDVTYTTQS
                     FVPEVDPSIGFYVDATYIVHFAAPKALVTNEGNDRATMRFVYDDNEYTDYTNLVTKFA
                     VKNTNLDMDEMINSEFPAWFTISRDPVSGDLISAPTPIVNPTKVVFDPSFKDFKGLTG
                     TAM"
     gene            2320..2850
                     /locus_tag="AC04VQ_00310"
     CDS             2320..2850
                     /locus_tag="AC04VQ_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552715.1"
                     /translation="MFGNGGTIEAMNSGQGSYNDPLKSIDLSSWVSNKKLNDVSAFGM
                     FAGCTGLNNLVFPKVTADKTHAFGDNTVDMSGIFIACIALRSFDMNKSFGANAEKLDF
                     AFAFSTAVTELDFDKTDAKELMFGSKASSMVRFAAMCTNLRVLNMKNFSAANVKEATG
                     AFMMSTSLVKFVIDDN"
     gene            2869..3600
                     /locus_tag="AC04VQ_00315"
     CDS             2869..3600
                     /locus_tag="AC04VQ_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552716.1"
                     /translation="MDQGLTISNTWFKYTPDKTPPYTQYASKDIPSVSKGGTFTRDSA
                     KAVYTPGVDGNNATFKFYYDHEVHNEGTEFSVPLSSNKGGMSPASSTKIFPDWYEVHT
                     YLNNKESDLASLMSGSLEALLGNDIDIKIKLLLPTQDCDVTFDPSFIGCENLKDVHYW
                     FVADSYGNAPFISYSGFKNFPTDELIDISEMFGGCSTEAMLCRVIPAYKYKIAQVGDT
                     SFLTNSDTYNTRLTTLDLSGCELPF"
     gene            3625..3951
                     /locus_tag="AC04VQ_00320"
                     /pseudo
     CDS             3625..3951
                     /locus_tag="AC04VQ_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024702.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
     gene            4090..4698
                     /locus_tag="AC04VQ_00325"
     CDS             4090..4698
                     /locus_tag="AC04VQ_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552717.1"
                     /translation="MTATPVHGTFTREAPTSKREIELGKSPEQGGTISPADTTITVYG
                     DTKVSVYKNTLIVNDKQYTPTATSPYVFDCWTINGKRIQPSHFMSATPDEIILLKFDD
                     EIWQTGVEIKAVFVNENLKARAVYETNKTVEGKTVNTLTYYYDENLHEEGQVFEVKNK
                     TGLDISSPDNITLETPSWIDSTFELDLKKLQKEGTGLNISSK"
     gene            4777..4920
                     /locus_tag="AC04VQ_00330"
     CDS             4777..4920
                     /locus_tag="AC04VQ_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552718.1"
                     /translation="MADHDNYQPVEKFYSPAAGQSPSSANEGFANLYSDNITNMQYAF
                     GGF"
     gene            4927..5403
                     /locus_tag="AC04VQ_00335"
     CDS             4927..5403
                     /locus_tag="AC04VQ_00335"
                     /inference="COORDINATES: protein motif:HMM:NF015347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HIO9552719.1"
                     /translation="MGSKIIQDTYADMLQTYGRSIQKLDVSALNFKKVTDMSFVFANC
                     LGLKEVNLGENFDSSNARAMTGMFFNCENLGIESATNGIINFDKITTPKVQDVSYMFF
                     GCCNIKELDLSKFNLNAATSREGKGQYPNSGAMFYDCTRLQKITIDSKLDNGFASG"
     gene            5689..5814
                     /locus_tag="AC04VQ_00340"
     CDS             5689..5814
                     /locus_tag="AC04VQ_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552720.1"
                     /translation="MDYNYIQSATMSVDQKEDNVLWADNYKIVATPKTDAYKFLY"
     gene            5869..6093
                     /locus_tag="AC04VQ_00345"
     CDS             5869..6093
                     /locus_tag="AC04VQ_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552721.1"
                     /translation="MLDELGPIDPSKTGRSFKAIFGDAILSAKALYTSPENGSNVGHL
                     KFVYDKSEHEGEADYIYEVSGNFNEYNYPS"
     gene            6250..7488
                     /locus_tag="AC04VQ_00350"
     CDS             6250..7488
                     /locus_tag="AC04VQ_00350"
                     /inference="COORDINATES: protein motif:HMM:NF015347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HIO9552722.1"
                     /translation="MPALTADASGLIVFGEENTSTTPPSIDFTLNGAQYINTTNINNV
                     KYMFGGMEFSNDYNGSPVNKINITGFDKTASLVNLEGMFANCTLLSDIVWTHDESYGS
                     DIKNFKKLFFNCRYLTGDKLAAAMSLINFKAATDISYMFGRYYFPTSTFSVTDEIEYN
                     LGNIYFKNDDVIAEGLFDGVKFTKKIIVKDNSTDNGCIASYKGAFGNCSNLETVDFTG
                     AKAFSTTNTVDMSYMFSGCSKLTSVNFINMTAGSTNLIIGGKTQTLAYMFENCTSLKQ
                     FDGVSFDITSVVSMEGMFYNTGFETFVFKDIFSKSSYDSALQNITYMLCGCSNLTYAS
                     LNRNAPQGAQLPKNLDMRYVMANCPSLTEVDLSGIHTGKYIIKAPVRSLGNVGMFAFI
                     DSNGNETPNNNIVDFAFDKE"
     gene            7507..7737
                     /locus_tag="AC04VQ_00355"
     CDS             7507..7737
                     /locus_tag="AC04VQ_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552723.1"
                     /translation="MYDTGLPFYTGQTQALYPSVMQESDIPADFSYDKLIYWYYKSSE
                     IIEAISIMELEKAGEYTLSEPHNLPFTAENPQ"
     gene            8011..8220
                     /locus_tag="AC04VQ_00360"
     CDS             8011..8220
                     /locus_tag="AC04VQ_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552724.1"
                     /translation="MIAKYDTASTTLTIGVDKYNEAESNVWAIDIDGYSPENLPGWHE
                     VANNVTSVSFMSNMKDVTSITSMAY"
     gene            8296..8547
                     /locus_tag="AC04VQ_00365"
     CDS             8296..8547
                     /locus_tag="AC04VQ_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552725.1"
                     /translation="MFKNCTSLTSVDFPCDVDYLTNLSTKDMFNGCSTLKSFSLGDMD
                     TFSEFSNMFTGCNSIEYLYFRQAYLDFSQAGLSQDPNAK"
     gene            8785..8994
                     /locus_tag="AC04VQ_00370"
     CDS             8785..8994
                     /locus_tag="AC04VQ_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552726.1"
                     /translation="MYFTFKFAPAANYAVSGMQRTDWSFEYQANILGEYYDTYILEGV
                     NINGVRSGMNQTGNVEANDEFQSIA"
     gene            9262..9459
                     /locus_tag="AC04VQ_00375"
     CDS             9262..9459
                     /locus_tag="AC04VQ_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552727.1"
                     /translation="MNNAYDLSQMFKNCPGISSIDFTGKTVIARSLYEMFAGCESLST
                     INLGDIRVGDTNVDGIFQNCN"
     gene            9712..10659
                     /locus_tag="AC04VQ_00380"
     CDS             9712..10659
                     /locus_tag="AC04VQ_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552728.1"
                     /translation="MYYKVENDKFDIPVLYIQTRAKAENDDYYTYEIMGLPNSDQEVS
                     KWTLDGQDITVGQEYAQLESGSHNFEVVFYEKPFNSAKVVYDTENNSIKFVYDAVEYA
                     KPQYEIRDVVGDVNLGTQPFSDTMRANAKTITFDESFYRYKGATNWNRYFADFSKLES
                     ITGLINICTDNLANVSGMFSVLPETFTSLDLNGLKLNGKNVDGDIDASDLISNCKGLT
                     NVTFTGSDFSKVGKLYYACSANPELAYVDVSGLDLSNVKTMSRMFSGNTKLEEIKFKN
                     DDGSSLFKCEMCTEYDMIFDHCVNLKKVELFFMAFYEPI"
     gene            10675..10848
                     /locus_tag="AC04VQ_00385"
     CDS             10675..10848
                     /locus_tag="AC04VQ_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552729.1"
                     /translation="MGGLCQNCPELREIDTTGARGDLYENYDGYFSWAHESEKLSKIT
                     FGDKFYSCIDYVL"
     gene            11473..12042
                     /locus_tag="AC04VQ_00390"
     CDS             11473..12042
                     /locus_tag="AC04VQ_00390"
                     /inference="COORDINATES: protein motif:HMM:NF015347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HIO9552730.1"
                     /translation="MNFKYLKTLDCFFARMHSTEFIGLQYINCEKVTSAKYMFYNCNT
                     VTNLDVSGLDTSHVVYMCDMFKDVSQVESIDFISKTGGPNNFATNKVRYMEFMFHNCY
                     QLQKLDMSSFNFDQPIISLREFMWNCYNLTEVNFENFWAAEYIEDADTSTNVSWFFEN
                     CSKLRKFTMGANFEKSLSEMALNHSGYES"
     gene            12193..12342
                     /locus_tag="AC04VQ_00395"
     CDS             12193..12342
                     /locus_tag="AC04VQ_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HIO9552731.1"
                     /translation="MDPVDPQVDPVVPPEEEEGAPLAAAALALPALAALGFRKRLRGK
                     HARIK"
     gene            12500..12922
                     /locus_tag="AC04VQ_00400"
     CDS             12500..12922
                     /locus_tag="AC04VQ_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="HIO9552732.1"
                     /translation="MIDKDEWLNTPEPTEDPSPEYIIMGDPDKEHREAAWRIAIGLQD
                     VDHLKPSEYLIEIANKNIDGIITSIEAREMIDEYYEKKKRYSDKNRSKEADLVASRIN
                     SMIQSVGNSFSVGSYLATHHNLFKGIYEFAGKFRDYDI"
     gene            13223..13573
                     /locus_tag="AC04VQ_00405"
     CDS             13223..13573
                     /locus_tag="AC04VQ_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013231.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIO9552733.1"
                     /translation="MRANYDNPAKGIRATTYYLERFLENLMFGTKHELHNRDIHVNNI
                     EIAKVFDVPSASANELTMFELRVLEQIKKNPEIKQAEIAKKVGRSLRTVKSLTKSLVN
                     KGAIKRENGKRYGY"
     gene            13642..14871
                     /locus_tag="AC04VQ_00410"
     CDS             13642..14871
                     /locus_tag="AC04VQ_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIO9552734.1"
                     /translation="MSNEKKIELLAPAGGPDQLRAAVNFGADAVYLAAEHFGMRAAAE
                     NFNAKTLAGGIKFAHEHNVKVYVTCNILMHDADIENLGAFIKQLSEAEADAVIVGDLG
                     ALATVREVAPNLDIHISTQASVANTASAIEYAKMGAKRIILAREMSLKEIAGLRKNLD
                     AAGYEKLDLEAFVHGAMCMSVSGRCLISSYLTGRSANKGACTQPCRWGYKLLEEKRDG
                     QEFEIGEDGFGTYIMNSKDMNMLEHIDDLKAAGLSSIKIEGRNKKALYVATVVNAYRH
                     VLDGEDAAKWTPELENVSHRPYSTGFYFGPAEQSPDYDGYEQNCVHVGDVLEDGKILC
                     RNHFEEGETLEALIPGGDIASIKVQDLMWFPDAKSGGKPETVANRATEIYSFVTKTPL
                     PPGTLLRNKTYLRTSRQ"
     gene            15000..15245
                     /locus_tag="AC04VQ_00415"
     CDS             15000..15245
                     /locus_tag="AC04VQ_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIO9552735.1"
                     /translation="MEFDLDIALEFVEKVNPEPTPIEYITGQTGDVMLPVILAAIMLL
                     LVSFGAFMFFKKRKARINEASSATAAATKVASLMTSK"
     gene            15258..15896
                     /locus_tag="AC04VQ_00420"
     CDS             15258..15896
                     /locus_tag="AC04VQ_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552736.1"
                     /translation="MVATLITAVALFAGSIVQAQADGEGKIETPSTVKAYVDKEAGTV
                     SFDTSWIKNISSGNIIFDKSQVVQVKESLEICDLSNSNFKVNALGGTVFNGHPNDETP
                     YIPTDLTALPPEETSDMTFDLTNVSATDLFNLIGHKVFNIKFWYLDSAIPVPQPNKLT
                     YNGAVQTGVNEGIGYTLSGTTETKDAGTYKATASLISGFVWEDGSHDDKIIN"
     gene            15945..16454
                     /locus_tag="AC04VQ_00425"
     CDS             15945..16454
                     /locus_tag="AC04VQ_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552737.1"
                     /translation="MYDGESHCFQEVDVVSGSFAGDEFFVYDNFPSIMYYSEAGISNS
                     FTYNGGSGTDTNNYKITATFGTLKITKVNVPIIVTANSDDKIYDGTELRNDRYTYNDE
                     VLVDGDSLTANVNGSITDAGTVSNVVTEIIIEKGTEDVSDNYTIGEPVNGTLTINKRD
                     ISISSKEQT"
     gene            16563..16988
                     /locus_tag="AC04VQ_00430"
     CDS             16563..16988
                     /locus_tag="AC04VQ_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552738.1"
                     /translation="MGGVDNSFKYKAIANTNLDNYNLNVSFKKLFIKKIETPIDVTAE
                     TISKEYDGALLSSNNVDYDKSILKGSDQLKATTSGSIIQAGSVTNVIDDVKIYRDGVD
                     ATANYTIGKFTSGILTVNKRSISVSSEDKTFTYNGKAQS"
     gene            complement(16979..17131)
                     /locus_tag="AC04VQ_00435"
     CDS             complement(16979..17131)
                     /locus_tag="AC04VQ_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552739.1"
                     /translation="MSKLVPTIGKQTNSLSTPSSLTFVTDVKLPKVAPSPSAQDPEIT
                     EALSQL"
     gene            17166..17474
                     /locus_tag="AC04VQ_00440"
     CDS             17166..17474
                     /locus_tag="AC04VQ_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552740.1"
                     /translation="MIKPSTKIVVTANSSTREYDGTELTNPGYTYTQGVLAEGDVLYA
                     DVEGSAFFAGDVVENVVKSVTILRGSEDVTDNYDLDEFVKGTLTVTKRKVVLESQSHD
                     "
     gene            17802..18011
                     /locus_tag="AC04VQ_00445"
     CDS             17802..18011
                     /locus_tag="AC04VQ_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552741.1"
                     /translation="MSVSIEGSIKNFGSVENIVKEVKVMRGGANVSGNYTFGEHVNGS
                     LKIYKRNITLESESKSFTYDGEKHN"
     gene            18822..19523
                     /locus_tag="AC04VQ_00450"
     CDS             18822..19523
                     /locus_tag="AC04VQ_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552742.1"
                     /translation="MENDTLNITISGSITKVGYANNIVSALSITRGGDDVSSNYSFGT
                     PNVGVLTVKPRPITLTSGTSEVEYSAQVLKNENISVGGEGWVVGESAVYSGFAEILDV
                     GSVSNTFTYDAGENTNLDNYTITPVYGTLTVNKVSTELIVTANSNEKTYDGTALTDSG
                     IKFSPALKGNNETVEAEIVGSQTDAGTSENSVDKVKILRGNVDITKNYTLGTYINGTL
                     TVKPRPITLISSPGE"
     gene            19674..20036
                     /locus_tag="AC04VQ_00455"
     CDS             19674..20036
                     /locus_tag="AC04VQ_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552743.1"
                     /translation="MVGTNLDNYTINKQCGKLKINKLTATLTVTANDASKPYDGTALT
                     QSDYSYTDGVIQTRDTLSAETEGSIINYGNTPNTIKTISITRDGVDVSYNYENIKKSA
                     GTLTITKRPITVKSKDGI"
     gene            20175..20342
                     /locus_tag="AC04VQ_00460"
     CDS             20175..20342
                     /locus_tag="AC04VQ_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552744.1"
                     /translation="MSPKGETKFGNYEITQKYGMLSITPRNITLTSGTATMTYNGEAQ
                     KYENVNVTTGS"
     gene            20439..21092
                     /locus_tag="AC04VQ_00465"
     CDS             20439..21092
                     /locus_tag="AC04VQ_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552745.1"
                     /translation="MTKAANYNITKKYGKISIKKIETPLIVTANSSSHTYDGNAYTDS
                     GFTYSDNIVKPLDSVSALVSGTITDVGSADNFVTSVRVLRDNDDISDNYTIGEYVKGT
                     LTVTPRDITITSGTKSMTYNGYAQKYESASVTSGSFVSGQGATYSGFASVTFVSEGEV
                     DNEFSYSLNSNTKEQNYNITQVNGKISIKKITTPLIVTANSNSKDYDGTPLTDSGFT"
     gene            21933..22046
                     /locus_tag="AC04VQ_00470"
                     /pseudo
     CDS             21933..22046
                     /locus_tag="AC04VQ_00470"
                     /inference="COORDINATES: protein motif:HMM:NF015347.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
     gene            22050..22502
                     /locus_tag="AC04VQ_00475"
     CDS             22050..22502
                     /locus_tag="AC04VQ_00475"
                     /inference="COORDINATES: protein motif:HMM:NF015347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HIO9552746.1"
                     /translation="MNSDFSFLKTGAVTTMYRMFYNCKNIENIDLSSFDTSNVTNMEG
                     MFSDCYNLKNINISSFNTSKVTTMASMFANSEEGLEVLDLSSFDTSSVVNMTAMFSGC
                     KYLRTIFVSSDFVTTKIEGAYGDSMFDRCDELIGGRGTSPGPTDYTYA"
     gene            22803..23102
                     /locus_tag="AC04VQ_00480"
     CDS             22803..23102
                     /locus_tag="AC04VQ_00480"
                     /inference="COORDINATES: protein motif:HMM:NF015347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HIO9552747.1"
                     /translation="MSSLQLTFSGCANIENLDVSTFDTSNVTTINGTFKGCSSLTFLD
                     LSNWNVSKVTAVASLFANCTNLVSFEKPKNSVAKITSNATAVFGGCESLKNIELD"
     gene            23196..23441
                     /locus_tag="AC04VQ_00485"
     CDS             23196..23441
                     /locus_tag="AC04VQ_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552748.1"
                     /translation="MKLTIGMFSGCSNLRSIIVGEDFHFSSVLNSALSKNMFFGCNNL
                     IGGRGFAFDESGPVDFTYAYIDLPGKPGYFTGPSSLY"
     gene            23784..23960
                     /locus_tag="AC04VQ_00490"
                     /pseudo
     CDS             23784..23960
                     /locus_tag="AC04VQ_00490"
                     /inference="COORDINATES: protein motif:HMM:NF015347.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
     gene            24369..24821
                     /locus_tag="AC04VQ_00495"
                     /pseudo
     CDS             24369..24821
                     /locus_tag="AC04VQ_00495"
                     /inference="COORDINATES: protein motif:HMM:NF015347.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
     gene            25143..25676
                     /locus_tag="AC04VQ_00500"
     CDS             25143..25676
                     /locus_tag="AC04VQ_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552749.1"
                     /translation="MFNGCSALEVLDLSSFVTNKDLSTFSVSMFAGCGNLKTIYVNEN
                     FCEFKTSMQMFKSCLSLVGGKGTKFDPNHIDSDYARIDQGAFNPGYFTSKDPAAVTMP
                     NDGLLLNNPNAQAPETQEADPQAPVNDPEVNPTPTESGGEGVPAGGEAAALVGVPLGL
                     AALGFRRRISGNHVKIK"
     gene            25898..26860
                     /gene="ftsE"
                     /locus_tag="AC04VQ_00505"
     CDS             25898..26860
                     /gene="ftsE"
                     /locus_tag="AC04VQ_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIO9552750.1"
                     /translation="MAKQVDTSVPTNPIPNDPGTTAPVRPSGASGRDLTPDGGTLESG
                     LVSGEDVAASSLTRGTSRNISMDGAGGGLNLPQVNVAAENANAARPVITFRNVTKVYA
                     AQPTKPALKDIDLQIFAGEFVFLVGHSGSGKSTFIRLITRELKATRGQLLIADEDLGT
                     MKNWRVPYLRRNIGCVFQDFKLLPNKTVFENVAFALEVIGKSRHVVKTQVPEVLRLVG
                     LGEKLQKYPDQLSGGEQQRVSIARAIVNRPPMLICDEPTGNLDPQTSRGIMDLLERIN
                     KTGTTVLVATHDREMVNSMRRRVVALDHGVLTRDQDRGVYGSNV"
     gene            26853..27767
                     /gene="ftsX"
                     /locus_tag="AC04VQ_00510"
     CDS             26853..27767
                     /gene="ftsX"
                     /locus_tag="AC04VQ_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HIO9552751.1"
                     /translation="MSSLYYFLREALIGFKRNLSTAIGSIITIFLSLLMIGLFLVGGL
                     IVNNIVSNVESEVMITCYVADGTQEADVKKLMNEAKDIDEVEDVTFTTKDQALEKFRN
                     SMSSSPEIVDQLDGQNPLPASVNVELKNPQDVSKVAEQINKSNNFKKIAENQAKPEDS
                     LKYGQKTVDQLFALTNVLRIVGICIIALLIFIAMIFINNTIRLAILARRKEIAIMRLV
                     GASNGFIRGPFFMESILHSLIGSLAAILIMEVLRSFALPAMVNALAWLPVDLSLATFL
                     IIYGILLVAGIIIGLIGSALAMRRYLSV"
     gene            complement(28072..28416)
                     /gene="rnpB"
                     /locus_tag="AC04VQ_00515"
     ncRNA           complement(28072..28416)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC04VQ_00515"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            28598..30454
                     /locus_tag="AC04VQ_00520"
     CDS             28598..30454
                     /locus_tag="AC04VQ_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012799634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIO9552752.1"
                     /translation="MAVEKVEKMPLAGATNGELAVAEITKALNNRMAAGSLGSCPVEF
                     TKAFVEACGSQSCGKCTPCRIGLKQMAKILGEILEGEACECKLKVLPDLASAIYESAD
                     CAIGFEAGRQVLESLKKNDDDYKYHLHHDGSCMYEKRAQVPCVEGCPAHVDIPGYIAC
                     IQAGRYTDAVRVIRKDNPLPLACGLICEHPCELSCRRNIVDDAINIRGLKRYAVDHAG
                     TYTQRKYPATRKKVAVIGGGPSGLTAAYYLTIMGHDVTIFEQRSHLGGMMRYGIPAYR
                     FPRKDMDKEINWLLKQGIKVKYNTNVPRDVSVAELRKKFDATYIAIGAHSGNSLRIPG
                     ENAEGVLSAVQLLGAIGDGKKLSFKGEDVVVVGGGNVAMDCARSAVRLGAKSVTIAYR
                     RRKDDVTCQMEELDGALEEGCHLLELHAPVEVMTKNDKVTGIKLQPQVSGPVDRGRPK
                     PMPANQKPVVVKADKILVAIGQAIDSAKFEKAGLGVNRRRFVADKFTEALDKKGNVIK
                     GVFVGGDCESGPATVIKAVGAGKVAARNIDNYLGFDHKITLEVKIPEARVGDKKAAGR
                     VKLMERSAAERKDDFNLMEKPMSDQECLLECNRCLRCDHSGMGSFRGGRELS"
     gene            30451..32175
                     /locus_tag="AC04VQ_00525"
     CDS             30451..32175
                     /locus_tag="AC04VQ_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013979953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HIO9552753.1"
                     /translation="MINFKINGQKLKASAGTTILQAARKNKIDIKTLCEYEKLNCIGA
                     CRLCMVEVKGFDRLVAACNTLVEEGMDILTDTPKTRRAVQTNLELILSEHNTDCTKCV
                     RNGNCKLQQLAEDHNIHKNIFGEEFKKVSWPRSFPLQRDESKCVKCMRCIQVCDNITG
                     AKVWELKNHGTRTRVGVASDLKIDATKCVLCGQCITHCPVGALSERDDIHQLQRALDD
                     PEITTVVQVAPAVRTAWAEEMGLSRKQATMQHMVAALKAIGFDYVFDTDFAADLTIME
                     EGTEFLTRLKHKKNYAWPMFTSCCPGWIRHMKHNRPNQIKQLSTAKSPQQMFGAACKE
                     LFKTQYKIAPEKVYCVSIMPCLAKKYECDVPELNDVKRGVKDVDASLTVREFQRMMKM
                     HNIHPADLKPVPFDNPLGEATGAAHIFGATGGVMEAALRTAYFVVNKKNPPADLFKSV
                     RGMDGWKEATFKLAGTPVRAAVVSGLVNTEKLLTALDNGEVEYDFVEVMTCPGGCAGG
                     GGQPIKEGVEMAKVRGDHLYFLDKKSKSRFSHENKSVQEIYKQIFKKPLSEKSHKLLH
                     TDQSKWSI"
     gene            32274..32348
                     /locus_tag="AC04VQ_00530"
     tRNA            32274..32348
                     /locus_tag="AC04VQ_00530"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32306..32308,aa:Val,seq:tac)
BASE COUNT        10260 a         5661 c         6857 g        10027 t
ORIGIN      
        1 ctcgtcgggc tcataacccg aaggtcggag gttcaaatcc tccccccgcg accatttccc
       61 caaattccaa aaatcaagaa aattcgcaca aaactcctgc tatctgttat aatatgttcg
      121 gttatggact tttgttcata aaataaaata ttaggggtaa agaaaggtaa taacatgaat
      181 gctttagaac caatgcaaac cgtatttcac tttattttta aggaagccgg cggcggtggc
      241 ggcggtggcg gagaagacat cgaagttgtc tctgcagctg ctcaaactgg tgattggctc
      301 acaattttgc ttgtggcgct ttttgctatt gcggcaattg cttcgcttgc gtttgttttc
      361 atgaaaaaca aacaatcttg aagcaacgcc cgaagcaatg caagccatgc tacagcaaag
      421 tcaaagacaa aaatttcgac atttgttgcg gtcgcaattg cgacgctcgc tctcgctgga
      481 gctgcaattt ctgttgcaaa cgcaccagcg cttcgtgctt ttgcgaatgg aaacgcaaat
      541 gcagacatgg attacaaagc cgaaattgac gtaattgttg atgaagctac gcaatcaata
      601 agcttcgaca ctaattattt tacaaatctt tcagctgatc ctgtccatat tgacaattcg
      661 acactgactt tatctgacga ggctaaactg ctggacatcc cagagtttgt atttaaagca
      721 tcgtatgaaa atgataaagc taatgttttt gaaggaatgt cggacgaaac cactccggat
      781 attccaactg gacaaactgt attggcaaca aaagctaccc aaaacataaa gtattcaact
      841 aatatgactt atgaaacagc taagctatta agtacaattg aaaatgcatt cactcttgaa
      901 cttaatgaaa ccccttgtta tacaattgaa tatagtgcaa atgaagctac aggtggcgaa
      961 gcaccagctc ctgtaacttt aactccaagt gcagaaactg gcgcgcttaa tgtagaaacc
     1021 tatgccccag aaaatacagg aggacttgta aagacaggat atcgcttcac tggttgaaat
     1081 actgccgcag atggaagcgg aacacactat aatccgggcg ataaaattga aagttcttct
     1141 gcaactgtag gtcaacaagt taatatttac gctgaatgag aagaaggtta cattgttact
     1201 tttgagggag accaatacat tgatgttgta gaagtcagtg gaaatactaa cgcttcattt
     1261 acgcacttta ttcctaaaac acacgcagct ggtgaatata caacttttgc agtaagcaag
     1321 gaagcagcaa ccctaaatta tttaacattt acatgaaaag acaaggctgg tgctgttcaa
     1381 acatatacag ttaagcctgt tccaaaagtt gctggtcata attttgacaa atggcttaag
     1441 gacgatgagt ctttgccttt atcacctaac cttacaaaag actcgaaatt tactgctaca
     1501 agtgttgaat cagttgtaac aatcacatat aaagttgttg acgaaaattt tcctcatggt
     1561 tcaatttctc taaacaactc agaagaaaat aacgattcgg agggatttgc cgctggagtt
     1621 gtcacagaaa catttaaacc acagttgggt gaaactccaa aaggagccac agcaactcct
     1681 gatacatcaa ctgattataa atatgatttt ataaactgga caaaagtagg ttctgacgtt
     1741 acatacacaa cgcagtcttt tgttcctgaa gttgatccaa gcattggttt ttatgttgat
     1801 gcaacataca ttgttcattt tgctgcacct aaggctctag ttactaatga aggtaatgac
     1861 agggcaacaa tgcgctttgt ttatgatgat aatgaatata cggactatac caatttagta
     1921 acaaagttcg ctgtaaagaa tactaattta gatatggatg agatgatcaa ttctgaattt
     1981 cctgcttggt tcacaatctc aagagatcca gttagcggag atttaatttc agcaccaact
     2041 ccaattgtta atccaactaa agttgttttt gatccttcat ttaaagactt caagggttta
     2101 acaggaacag ccatgtgatt tatggacatg aaagctatat cgcttgcaca agaaatgtta
     2161 ccaagcgcta tgtcagatag ctgaaataat tttcattcag gttcttatag ttcacttggg
     2221 gcgcctttaa agccagtagt aaaaggcgca caaagacttc ttgaaagtgt tgagggatta
     2281 gaaaatgttt gcatagacaa cttaactaca acagatttaa tgtttggaaa tggtggcaca
     2341 attgaagcaa tgaatagtgg tcaaggttca tacaatgatc cacttaaaag cattgatctt
     2401 tcaagctggg tatcaaataa gaagttaaat gatgtttctg cctttggtat gtttgctggt
     2461 tgcacgggat taaataacct cgtatttcca aaagtaaccg cagataaaac tcatgccttt
     2521 ggagacaata ctgtagatat gtctggtatt tttattgctt gtatagcttt aaggtctttc
     2581 gacatgaaca agagttttgg tgctaatgcc gaaaagctcg actttgcttt tgcgttttct
     2641 actgccgtta ctgaattaga ttttgacaaa acagatgcta aggaattaat gttcggctca
     2701 aaagcttcgt caatggttcg ctttgccgct atgtgtacaa acttaagagt tttgaacatg
     2761 aaaaacttta gtgccgcaaa tgtaaaagaa gctacagggg cgtttatgat gtctacgtct
     2821 cttgtcaaat ttgttataga cgataattga agaattccat attcaaccat ggatcaaggt
     2881 ttaacaattt ctaatacttg gtttaaatat acaccagaca aaacacctcc ttatacacaa
     2941 tatgcaagta aggatattcc aagtgtttct aaaggtggta cttttacaag agattctgcg
     3001 aaagctgtct acacacctgg agttgatggc aataacgcaa catttaaatt ctattatgat
     3061 cacgaagttc ataacgaagg tacagaattc tccgtaccac tttcttcaaa taagggagga
     3121 atgtctccgg cgtcttcgac taaaattttc ccagattggt atgaggtaca tacctatcta
     3181 aataacaaag aatcagacct agcttctctt atgtctggaa gccttgaggc attacttgga
     3241 aacgatattg atataaagat caaactgctg ttgcctactc aggattgtga cgttacgttt
     3301 gatccgtcct ttataggctg tgaaaatctt aaagatgtcc attattggtt cgttgctgac
     3361 tcatacggta atgctccttt tatatcatat tctggattta aaaacttccc aactgacgaa
     3421 ttgatagata tttcagagat gtttggtggt tgttcaacag aagcaatgtt gtgcagggtt
     3481 attcctgctt ataaatataa aattgcgcaa gtgggcgaca cttcatttct tactaatagt
     3541 gatacttata acacaaggct taccactctt gacttaagtg gttgtgagct acctttctga
     3601 catggtcttg tccagaattg cccaaacctt aatactgtta ttcttccgca atcagttcag
     3661 gtattgaata tggcttatgc tttttacaat tgtaaatctt taaagacaat aacaaactta
     3721 gatactatta atactgctta ttgctatcaa tatacgaagg cgtttgctgg ttgttcttct
     3781 ttagaaagtc ttgatcttag cagctttaac acaaacggct tgacagcgaa gtacggtaat
     3841 gatagagact ctattaagac tgctgtatac ggaatgttcc aaggatgtta ttcgcttaaa
     3901 gaatttaaga ctgcagctta cgactcaacc aattttaaag atccaacctg aggcgttata
     3961 atgaaggatg cttctttgcc tgatgatcat ggaatttctc ctgatgaatc tgatgataat
     4021 ccttgaattg ctaatgattt tgaaaataag cctgtttggg ctaaagatat tccttgagaa
     4081 aatgaagcca tgactgcgac tcctgttcac ggaacattta cacgtgaggc acctacaagt
     4141 aaaagagaaa ttgaattggg taaatcccca gaacaaggcg gcacgataag tcctgccgat
     4201 acaactatta cagtatatgg ggacactaaa gtttctgttt ataaaaacac attaattgta
     4261 aatgataaac aatatactcc aactgctacg tcaccttacg tttttgattg ctggacaata
     4321 aatggtaaaa gaattcagcc atcgcacttt atgtctgcaa caccagatga aattatccta
     4381 ctaaaattcg atgacgaaat ttggcaaact ggagtagaaa ttaaagctgt atttgttaac
     4441 gaaaacttaa aagcaagagc agtatatgaa actaataaaa ctgtcgaagg aaaaactgtt
     4501 aacacactta catattatta tgacgaaaac ttacacgaag aaggacaggt ttttgaagtt
     4561 aagaacaaaa ctggtttaga tatttctagc cctgacaata tcactttaga aactccaagt
     4621 tggattgatt caacatttga gcttgacctt aagaaattac aaaaagaagg aacgggctta
     4681 aatattagtt caaaatgaaa gattgatcct aattgtgtta ttttcgatag tacgtttaag
     4741 cagtatcaat cattagaaag tatttctatg tgatggatgg ctgatcacga caattatcaa
     4801 cctgtagaga aattctatag tcctgctgct ggacagtctc caagttctgc aaacgaaggt
     4861 tttgctaatt tgtacagtga caacattaca aacatgcaat atgcctttgg tggtttttga
     4921 attgaaatgg gttcaaagat aatccaagat acttatgccg acatgttaca aacatacggc
     4981 agaagtattc aaaaacttga cgtgtcagca cttaatttca aaaaagttac tgatatgtct
     5041 tttgtgtttg ctaattgttt aggtcttaaa gaagttaact tgggtgaaaa ttttgactca
     5101 tctaatgcca gggctatgac tggaatgttc tttaattgtg aaaatctcgg cattgaatct
     5161 gcaactaatg gaattataaa ctttgataaa attactactc caaaggttca agatgttagt
     5221 tatatgttct ttggttgttg caatataaaa gagcttgatt tgtctaaatt taatttaaat
     5281 gctgcaactt caagggaagg aaaaggtcaa tatccaaatt ctggggcaat gttttatgat
     5341 tgcacacgct tacaaaagat tactatagat tcaaaacttg ataatggatt tgcttcaggt
     5401 tgaactgtaa acattggaaa taatcaacca aatgaatctc cgtttaattt aagttgagtt
     5461 ggaggactaa aagaaaacct tgataacgat ggcaacgtcc agaagtggac taatactgca
     5521 tacgaaggaa gagaatttac gtcgcttcaa attccatggg attttaatca gtatggagaa
     5581 ggagaagttg aggaagttgt aagcggcact tttgttagag cgcaacctat tgaaaataag
     5641 actgtattca ctgctgacga aaacggtctt gttaacggaa aagattctgt ggattacaat
     5701 tatattcaaa gcgcaactat gtctgttgac cagaaagaag ataatgtact ttgggcagac
     5761 aattataaga tcgtagcaac tccaaagacc gatgcgtata agttcttgta ttgaactttt
     5821 ggtgaagaat gaggaactga tctttctggt aaaagagtaa ctcaagaatt gcttgatgaa
     5881 cttggaccga ttgacccatc taaaacagga cgcagtttca aagccatttt tggcgatgca
     5941 attttaagtg ccaaggcgct ctacactagc ccagaaaacg gaagtaatgt tggtcacctg
     6001 aagtttgttt atgataaatc tgagcatgaa ggtgaagcgg attatatata tgaagtaagt
     6061 ggtaacttta atgagtataa ttatccatca tgatataaaa attcagcata tcattttgtt
     6121 ccaagtgaca accacgcggt cccaattatt gatactttca ttgaagagag tggtgcaaaa
     6181 tacataactg ttgaaattga tagtaccttt agtagttttg acagcttaac aagtctttct
     6241 tgttggttta tgccagcgtt aactgctgat gccagtggat taattgtttt tggcgaagaa
     6301 aatacaagca ctacacctcc ttcgattgat tttacgctaa acggggctca gtatattaac
     6361 acaactaata ttaataatgt taaatatatg tttggcggta tggaatttag caatgactat
     6421 aatggctcac cggtaaacaa gattaatatt actggctttg ataaaactgc aagcttagtg
     6481 aatcttgaag gaatgtttgc aaattgtact ttattaagtg atattgtttg gactcacgac
     6541 gaatcgtatg gaagcgatat taaaaatttc aagaaactct tctttaattg cagatatttg
     6601 actggcgata aacttgcagc cgctatgagt ttgataaact ttaaagctgc aacagatatt
     6661 tcttatatgt ttggaagata ttatttccca acttcaacat ttagtgttac cgatgaaatt
     6721 gaatacaatt taggaaatat ttatttcaag aacgatgatg ttatagctga aggtttgttt
     6781 gatggtgtta agtttacaaa gaagataatt gtaaaagata actcaacaga caacggatgt
     6841 attgcttcgt ataagggagc ttttggaaat tgctctaatc ttgaaacagt tgattttaca
     6901 ggtgcaaaag ctttctctac aacaaacact gttgatatgt catatatgtt tagtggatgt
     6961 tctaaattga cttcggttaa ctttattaat atgacagctg gaagcaccaa tttaatcatc
     7021 ggcggaaaaa cacagacact cgcttacatg tttgaaaatt gtacttcttt aaaacaattt
     7081 gatggtgtaa gttttgatat tacaagtgtt gttagcatgg aaggtatgtt ctacaatact
     7141 ggttttgaaa cttttgtatt taaagacata ttcagcaaat caagctacga tagcgcactg
     7201 caaaatataa cttatatgct ctgtggttgt tctaatttaa catatgcttc tcttaacaga
     7261 aatgcgccac aaggagccca acttcctaaa aatctcgata tgcgttatgt catggcaaat
     7321 tgtccaagtt taactgaagt tgatttatcc ggaattcata ctggtaaata tataatcaag
     7381 gcacctgtca ggtcgcttgg caatgtagga atgtttgctt ttattgacag caacggtaat
     7441 gaaactccaa ataataatat tgttgatttt gcatttgata aggaatgaga agactctagg
     7501 tattctatgt atgatacagg ccttccattc tatacggggc agactcaagc gttgtatcca
     7561 agtgttatgc aagaatcaga cattcctgct gacttttcat acgataagct aatctattgg
     7621 tattataaat ctagtgaaat tattgaggct atcagtatca tggagcttga aaaagccggt
     7681 gaatatacat taagtgaacc acacaattta ccttttactg ctgaaaatcc tcaatgagga
     7741 atagtttgca ctgaaggtgg aggcgctctt aacaattttt attattatga cgatgtagaa
     7801 atttggcttg acactgatcc tgattttgta aacataaagt ataaagagtt aactgcaaaa
     7861 cttacataca gaacggccgc taattcaaca tttgtaaaat gaacatatgt taatgacagt
     7921 ggcgaagtta aggatctaac aactgatcac gttacattta cagggaatga aaataataag
     7981 cttactgcaa actttaaaga tggtaattta ttgattgcta aatacgatac agcttctaca
     8041 actctaacaa ttggtgttga taaatacaat gaagctgaaa gcaacgtatg ggcaattgat
     8101 attgacggtt attcaccaga gaatttacct ggatggcatg aagttgcgaa taatgtcaca
     8161 tctgttagct ttatgtcaaa catgaaagat gttacatcaa taacttctat ggcttattga
     8221 ttctgtgatt ttactaatct tcagaatata gagttgtctc aattacaatt tagcaaggtt
     8281 aaaagttttg acggcatgtt taagaattgc acttcattaa caagcgttga tttcccttgc
     8341 gatgttgatt atttaacaaa tttgtctact aaagatatgt ttaatggttg tagtactttg
     8401 aagtcgttta gtttaggcga tatggataca tttagtgaat ttagtaatat gtttactgga
     8461 tgtaacagta ttgaatattt atattttaga caagcatacc ttgattttag tcaagcaggt
     8521 ttatctcaag atccaaatgc taaatgattt agtttaggag caagcaataa tgaccggtat
     8581 agtgctgttt gctccacctc tgagatcaag cgtaacggtg gcggtgattt atacatacga
     8641 gtatctgata gcgttaattt aaccataaat aatactgacg tatgtaaatt tggaagcaaa
     8701 agaacaactt atgacagcgt tgaactagga attaatcctt cagctggtct tactgctaca
     8761 tatagaattg aagataatac tcgcatgtat ttcactttta aatttgctcc tgctgcaaat
     8821 tatgcggtaa gcggaatgca aagaactgat tggtctttcg aatatcaagc aaatattttg
     8881 ggtgaatatt atgatacgta tattttggag ggtgttaaca ttaacggtgt acgttcaggc
     8941 atgaaccaaa ctggcaatgt tgaggctaat gatgaatttc aatctatagc atgacaaaag
     9001 cgttatgcaa aagctaaata tgacaatggt gcattaactt tctattatga tgagcaggaa
     9061 gttgcacctg atgagtatgc tgtaaacggt acagcatgat atgacgaagg aaatcctcca
     9121 gcatggcatg gtgaaaagac aagtatttca aggcttgttt ttcagccaag ttttaaggat
     9181 tatactaata taaacaacac tcaatattgg tttggaggtt ttacagcgct tactactata
     9241 gatggttttg aaaacttgta tttgaataat gcatatgatt taagccagat gtttaaaaat
     9301 tgtcctggta tttcttctat tgactttact ggaaagactg ttattgcacg tagtttatac
     9361 gaaatgtttg ctgggtgcga aagcctttct actattaatc ttggtgatat acgtgttggc
     9421 gatacaaatg tagacggaat attccaaaat tgcaattgac ttaattctgt aacttttggt
     9481 gaaaattgaa agaaagcagt atatattcgc aactctcatg atacgtcata ttatataagt
     9541 gaggcaacag gtgcaagtta tattaacggt acgttcccat atgccaacaa tagtggatat
     9601 ggaacttttg ttattgctga ccaaagtaaa gttgccgcta ttaatccttc atcacaaggt
     9661 ggcggagaaa tttatggtgt taatcaaatt ttctatgatg ataatcatga tttgtactat
     9721 aaggtagaaa atgataaatt tgacattcct gtcttataca tacaaacaag agctaaagct
     9781 gaaaatgatg attattatac ttatgagatt atgggcttgc ctaattccga tcaagaagtt
     9841 tctaagtgga cattagacgg tcaagacatt actgttggtc aagaatatgc acaattagag
     9901 agcggttcac ataattttga ggttgtcttt tacgaaaaac cattcaatag tgccaaagtt
     9961 gtttatgaca cagaaaataa ttctattaag tttgtttatg atgctgtcga gtatgctaaa
    10021 ccacagtatg agatacgaga tgttgtcgga gatgttaatt tgggtactca accattttca
    10081 gatacgatgc gtgccaatgc taaaactatt acatttgatg aatcattcta ccgctacaaa
    10141 ggagcaacta attggaaccg ttattttgca gacttcagca aacttgaaag cattactgga
    10201 cttataaata tttgtacaga taatttagct aatgtttcgg gtatgttttc tgttttgcca
    10261 gaaactttca cctctcttga cttaaatgga ttaaagctca atggtaaaaa tgttgacgga
    10321 gatattgacg ccagcgattt aatttcgaat tgtaaaggat taactaatgt aacatttact
    10381 gggtcagatt ttagtaaggt aggaaagttg tattatgcat gttctgccaa tcctgaattg
    10441 gcctatgttg atgttagtgg tcttgattta agtaatgtaa aaacaatgtc tcgtatgttt
    10501 agtggcaata ctaagcttga agagattaag ttcaagaatg atgatggaag ttctttgttt
    10561 aaatgtgaaa tgtgtactga atacgacatg atttttgacc attgtgttaa tttgaagaag
    10621 gttgaactat tcttcatggc cttttatgaa cctatttgag gatataaacc taccatgggt
    10681 ggtttatgtc agaactgtcc tgaattaaga gagattgata ctacaggagc aaggggagat
    10741 ttatatgaaa actatgatgg ttatttttca tgggctcatg aaagtgaaaa gctatcaaaa
    10801 attacatttg gcgacaaatt ctactcgtgt atagattatg tcctttgaag agaatcatct
    10861 tatgactggg tcgattcaaa aggaaatcaa atttatactg gcaacaatta tggaccagga
    10921 acctattata aaattgctaa gattgaattc ggtgttaatg acgaagacgc tggcgaatta
    10981 ccagaaattg aaccactaat tcttcaaagc gcacttaaag gcgaatatgc aacttatact
    11041 acagatgacc ataatttaac agttaatgtt tgcaataaag agggcgcagt tacatcaaca
    11101 aagacatata cggttagtga tgttgatgtt gacaactata cattccaagg ttgggaattt
    11161 gatggtactg ttactgaagg taaaattgaa tcatcaggta aatttattgc aaaatatcag
    11221 agtaatgttc ctgctggtgc atattatttt gatgctacgg aaaatgaacc ttcgcgttta
    11281 gtatttatgt gtagccaaga tgaacctgag ggttatagat ataaatttga tattaaaaag
    11341 aatattaata ttgaatatga aatgtctaga gacggaaacg agcctaatga attccagcaa
    11401 tgaaacgaat atggcacacc gtggtattga gatgttatat atgcacaaca agttattatt
    11461 gacccaagtt ttattaactt taaatatcta aaaacacttg attgtttctt tgcaagaatg
    11521 catagtactg aatttattgg cttgcagtac ataaattgtg aaaaggttac ttcagcaaag
    11581 tatatgtttt ataattgtaa tactgtcaca aatcttgatg taagcggttt ggataccagt
    11641 catgttgttt atatgtgtga tatgtttaaa gatgtaagtc aagttgaaag tattgatttt
    11701 attagtaaaa ctggaggacc aaacaacttt gcaacaaata aggttcgtta tatggaattt
    11761 atgttccata actgttatca gcttcaaaaa ttggatatgt caagttttaa ttttgatcag
    11821 cctattattt ctttgcgtga gtttatgtgg aattgttata acttaaccga ggttaatttc
    11881 gagaattttt gggctgcgga atacatagaa gatgcagata cgtcaacaaa tgtttcttgg
    11941 ttcttcgaaa attgttctaa gttaagaaaa tttactatgg gtgctaattt cgagaagagc
    12001 ctttcggaaa tggctctaaa tcattctggc tacgagtcat gagttaatca gaacacgggt
    12061 aatgttttca atgttaataa ttgaggtcaa tgagaagctg gtacttatgt tcctgagggt
    12121 tatgttaata acgcaatcaa taacggttta aatcaatcta ttacgcaaga tgacccacag
    12181 gttgatccgc aagtggatcc tgtagaccca caagttgacc ctgtggttcc accagaggaa
    12241 gaggaaggtg cgccattagc agcagctgct ttggcgctcc ctgctttggc tgccctcgga
    12301 tttaggaagc gcctccgcgg taagcacgct cgcattaagt aactcgattt ccccggggcc
    12361 aggactcgat ttactcgtgg caaaaactcg attttaaggc cgggctaacc cccggccttc
    12421 tttataggta aacttttgca ctttggttgc actttggcgc aaagtgtagt atactcaatt
    12481 tcgcgaaaaa ggaggcgcca tgattgataa agatgaatgg cttaacacgc cagaaccgac
    12541 cgaagacccc agtccagagt atataattat gggcgatccg gacaaggagc atcgtgaggc
    12601 tgcatggcgc attgcaattg gtcttcaaga tgttgaccac ttaaagcctt cggaatacct
    12661 gatagagata gcgaataaga acatcgatgg aattattacg tctattgaag caagagaaat
    12721 gatcgatgaa tactatgaaa aaaagaagcg ttattcagat aaaaatagaa gtaaggaggc
    12781 tgacttggtt gcctcgcgta ttaattcaat gattcagtca gttggaaata gtttttctgt
    12841 tggatcttat ttagcaacac accataattt attcaagggt atttatgagt ttgctgggaa
    12901 atttcgtgat tatgatattt gaaaaagcga atgagtttta aatcaggaaa cggttgatta
    12961 tagtgagttt gaaaagttgg aggatgaagt tacgcgctgc atagaacaag aaaaagattt
    13021 tgatttcacc ataattgagc aagaagaata tgttaagcat atggctgatt ttgttgctaa
    13081 actttgaaaa atacatgctt ttggagaagg taatacaaga acaactgcag tattttttat
    13141 taaacgttta aagtcatttg ggtttgatta catcaataat gacatgttcg agaagcattc
    13201 gtggtatttt cgaaatgctc ttgtgcgtgc gaattacgat aatcctgcta agggaattcg
    13261 cgctactaca tattatttag agcggttttt ggagaacttg atgtttggta cgaagcacga
    13321 gcttcacaat agggatattc acgtaaacaa tattgaaatt gcgaaggtat ttgatgtgcc
    13381 ttctgcctcg gcaaatgagt tgacgatgtt cgagcttcgg gtgcttgagc aaattaagaa
    13441 gaatccagaa atcaaacaag ccgaaattgc gaagaaggtt gggcggtcgc ttcggaccgt
    13501 gaagagcctg acgaagtcgc tggtgaacaa gggtgcaatc aagcgcgaaa acggcaaacg
    13561 ctacggctac tgagttgttc aaatttagaa ggtgcatttc atgccaggca ataattacac
    13621 ctttttgtta taattgggcg cgtgagtaat gagaagaaaa tagagctttt ggcgccggca
    13681 ggtgggccgg atcagttgcg ggcggcggtg aacttcggcg cggatgctgt ttaccttgcg
    13741 gcggagcact tcggaatgcg cgcggctgct gaaaatttca acgctaaaac cctggcaggg
    13801 ggcataaaat tcgcgcatga gcacaacgtt aaggtctatg tgacatgcaa cattttgatg
    13861 cacgatgctg acatcgaaaa tttgggggct tttataaagc agctctccga agccgaggct
    13921 gacgctgtga tagttggtga ccttggtgcg ctggcgaccg ttcgtgaagt cgccccgaac
    13981 ctcgacattc atatttccac acaagcaagc gtggccaaca cggcttcggc gattgaatat
    14041 gcgaagatgg gtgcaaagcg aatcatcctc gcgcgcgaaa tgtcgctcaa ggaaattgcg
    14101 gggcttcgca aaaatttgga cgccgcgggc tatgaaaagc tggaccttga ggccttcgtt
    14161 cacggtgcaa tgtgtatgag cgtgagcggc cgatgcctaa tttcaagcta tctaactggg
    14221 cgttcggcga ataagggcgc gtgcactcag ccttgccgct ggggctataa gttgctcgaa
    14281 gaaaagcgcg acggccagga attcgaaatc ggcgaagacg gttttggcac ctacatcatg
    14341 aattccaaag acatgaacat gcttgagcac atcgatgatt tgaaagctgc ggggctttcc
    14401 tcgataaaaa ttgaaggccg caataaaaaa gctctttacg tggccacggt tgttaatgcc
    14461 taccgccatg ttcttgatgg cgaagacgcg gcgaagtgga cgcccgaact tgaaaatgtt
    14521 tcgcaccgtc cgtattcgac cggattttac tttggaccgg ctgagcaatc gccggattat
    14581 gatggttacg agcaaaattg cgtccacgtc ggagacgttt tggaagacgg taaaatcttg
    14641 tgccgcaatc atttcgaaga aggcgaaacc ctcgaagcgc taatcccagg tggagacatc
    14701 gcgagcatca aagttcaaga cctcatgtgg ttccctgacg caaaatctgg cggcaagcct
    14761 gaaactgttg caaaccgtgc aaccgaaatc tatagcttcg tcacaaaaac acctctccca
    14821 ccaggcactc tccttcgcaa caaaacttac ctccgcacct cccgccaata aaagatgtaa
    14881 ttcatgcctg gcaataatta caccttttgg tgcatttcat gcctggcaat aattacacct
    14941 aattggacat aaaacctggt attttgctat aatataaagg ttttcgaaag aagggtatta
    15001 tggaatttga tttagacatt gctttagaat ttgttgaaaa ggttaatccg gagcccacgc
    15061 ccattgaata cataacaggg caaactggcg atgtgatgct accagtcatt ttagctgcta
    15121 ttatgctgct tctagtttcg tttggagctt ttatgttttt caaaaagcgt aaagctcgta
    15181 ttaacgaagc ttcgtctgca actgcagccg cgacaaaagt tgcgtcgcta atgacaagca
    15241 agtgaattgc tcttgctgtg gttgcaacgc ttatcactgc tgtagcgctc ttcgcgggca
    15301 gtattgtgca agcccaagca gatggcgaag gcaagatcga aactccaagc acagtcaagg
    15361 cgtatgttga taaggaagct ggtacggtca gcttcgacac aagctggatt aaaaatatca
    15421 gctcaggtaa cataattttt gacaaaagcc aagttgtgca agttaaagaa tctcttgaaa
    15481 tttgtgatct aagtaattcc aattttaaag ttaatgcatt gggaggtact gtttttaatg
    15541 gccacccaaa tgacgaaacg ccatatattc caaccgacct tacagccctt ccccccgaag
    15601 aaacgtctga catgactttc gaccttacaa atgtgtcggc aaccgatctg ttcaatttaa
    15661 ttggccacaa ggttttcaat ataaagtttt ggtatttgga ttccgcaatt ccagtccctc
    15721 aaccaaataa gctcacttat aacggtgcag ttcaaacagg cgttaacgag ggcatcggct
    15781 acactctttc tggcaccacc gaaaccaagg acgccggaac ttataaggca actgcaagtt
    15841 taattagtgg cttcgtttgg gaagacggct cccacgatga caaaataatt aattgagaaa
    15901 tttcgcctcg tgatatttca atcgcaagtg aagatcattc atgaatgtat gacggcgaaa
    15961 gtcattgttt tcaagaagtt gatgtagtta gtggatcttt tgctggagat gaatttttcg
    16021 tttatgataa ctttccttca attatgtatt atagcgaggc tggaatttca aatagcttta
    16081 cttataatgg gggttccgga acagacacca acaattataa aattactgcc acgtttggca
    16141 ctttaaaaat aaccaaagtt aatgtcccta ttattgttac ggctaacagt gatgataaaa
    16201 tttacgatgg tactgagctg agaaatgatc gttacactta taacgacgaa gtacttgttg
    16261 atggtgattc tctaactgca aatgttaatg gttcaattac tgatgcagga acagtttcta
    16321 atgtagttac agaaataata attgaaaaag gaacagaaga tgtttccgat aactatacaa
    16381 ttggtgaacc tgttaatgga actttaacaa tcaataaacg tgatatttca atttcaagta
    16441 aagaacaaac ctgagattac aatgctcaag ctcatagttt tacagaaact gatgttactg
    16501 gagaatgggt tgaaggtcaa ggtattgact gttatgattt tgctaccata acagattata
    16561 ctttgggtgg agttgataac agttttaaat ataaggctat tgctaacact aatcttgata
    16621 attacaattt aaatgtttct tttaaaaaac tattcattaa aaaaattgaa actccaatag
    16681 acgttacagc tgaaactatt tctaaagaat acgatggtgc tctgctttca agcaataatg
    16741 ttgattatga caagtctata ttgaaaggtt cggatcaact taaagcaact acaagtggat
    16801 caattattca ggctggttct gtcactaatg ttatagatga tgtaaaaatt tatcgagacg
    16861 gtgttgatgc tactgcaaat tatactatag gtaaatttac tagcggaatc ttaacagtaa
    16921 ataaacgaag tatttcagtc tcaagcgaag ataaaacctt cacctataat ggcaaagctc
    16981 aaagttgaga taaggcttca gtaatttctg ggtcttgagc ggacggcgaa ggagcaacct
    17041 ttggtaattt tacatctgtt acaaacgtta acgaagaagg tgttgacaac gaatttgttt
    17101 gcttgccaat agttggaact aatttagaca attatgaaat ttcgtacaat tatggaactt
    17161 tgaaggtgat taagccatca actaaaattg tagttaccgc taatagttca acccgagaat
    17221 atgatggcac tgagcttact aatcctggct atacatatac tcaaggtgtt ttggcagaag
    17281 gtgatgtttt atatgctgat gttgaaggtt ctgcattttt cgcaggagat gttgttgaaa
    17341 atgttgttaa gagcgtaaca attttaagag gcagtgaaga tgtaaccgat aattatgatt
    17401 tagatgaatt tgttaagggt actttaactg ttacaaaacg taaagttgtg cttgaaagtc
    17461 aatcgcatga ttgagattat gattctacag cgcatacatg acctgaagtt actgttaagt
    17521 ctggttcttt tgttgccgga gaaggtcttg attataacaa ttgaacaagt attactgatt
    17581 atagtgatag tggtttacaa aatcagtttg cttattctaa aaagggcgaa acggatcttg
    17641 ataattatca aattatatta aattttggaa ctcttaaaat taaaaagatc acaacccaat
    17701 tagttgttac agctaatagt gcttataaaa catatgatgg aagtgatctt tcggacaatg
    17761 gatatacatt tacagaaggc ataattcaaa gcaatgattc attgagtgtt tctattgaag
    17821 gtagtattaa aaatttcggt tcagttgaaa atattgttaa agaagttaaa gttatgcgcg
    17881 gaggcgccaa cgtttctggt aactatactt ttggggaaca tgttaatggc tcattaaaaa
    17941 tctacaaacg taatattact cttgaaagtg aatctaagtc atttacatat gatggagaaa
    18001 aacataattg agataaggta aatattaaaa gcggagaatt cgttgaagga gaagaggttg
    18061 aatttgttga ttttccttat gttaaagata tttctgaagg agacgttcaa aatactttta
    18121 cagtaaagcc aataggcgaa acgattcttc ttgataacta taatattaaa aagaacattg
    18181 gtgttatacg aattgtaact aatgaaaccc ctattttaat tactgcaaat agtaaaaata
    18241 aagtttatga tggtacgcct ttaacagata atggatttac gtacactgaa ggtgttctag
    18301 ctactggaga tgttctgaca gcagatgttt ctggaacaat aacaaatgtt ggcaatcagg
    18361 caaatgttgt tgataaatct tctattaagg ttgtaaattc tgacggagaa gaagttacag
    18421 atagctatac attttcagat cctaaacccg gtttattaaa aattacaaaa agaagtataa
    18481 ctttaataag cggtactgat tcttgagatt atgattcaaa agaacattat tgcgatacag
    18541 tttctcttaa agaaggcgat tcatggatag gaagcgatgg tgttgaatgt tatgattttg
    18601 ttaaggtaat tgattacaaa gctgaaccaa caaaaaatga atttaagtac acaaagaaag
    18661 gcgattcaga tcttgacaat tatgatatta aacgtgaata tggaacagtt cagatcaaaa
    18721 aacttactac acctataatt atttctacaa gttctgatga aaaaacatat gatggaagtg
    18781 cactttctaa cagcggctat gaaatttcgc aaggtgttgt tatggagaac gacacgttaa
    18841 atattacaat ttcgggtagc attactaaag tcggctatgc aaacaatatt gtatcagctc
    18901 tctcaattac tcgtggtgga gatgatgtgt cgagcaacta tagcttcggt actcctaatg
    18961 ttggtgttct aactgtaaaa ccaagaccaa ttactttaac aagtggaact tctgaagtcg
    19021 aatatagtgc gcaggtattg aagaacgaaa acatttctgt tggtggcgaa ggctgggttg
    19081 taggtgaaag tgcagtttat tcgggatttg cagaaatttt ggacgtgggc tcagtaagca
    19141 atacatttac ttatgacgct ggcgaaaaca caaacttaga caattataca atcacacctg
    19201 tttatggaac cttgacggtt aataaggttt caactgagct tatagttacc gcaaatagca
    19261 acgaaaaaac ttatgatggt acagcattaa ccgattcagg cattaaattt tctcctgctt
    19321 taaagggaaa caatgaaact gtcgaagctg aaattgtagg ttcacaaaca gacgctggaa
    19381 cttcggaaaa cagtgtagat aaagtcaaaa tcttaagagg aaatgttgat attactaaga
    19441 attatacttt aggtacttat attaatggca ctttgactgt taaaccaaga cctattacct
    19501 taataagttc accaggagaa tgaacatatg attctcaaga acactttaac aaagaagtaa
    19561 aggcttctat agatactcct tgagttaaga atcaaggagc aacttactat aattttgcga
    19621 aaattactaa ttatatagaa ggtggtattg acaatacatt tgatgtcgaa gcaatggttg
    19681 gaacaaacct cgacaattat actattaata agcaatgtgg aaagttaaaa atcaataaac
    19741 taacagctac tttaacagta actgcaaatg atgcaagtaa gccatatgat ggaactgctt
    19801 tgacacaaag tgattattcg tatactgatg gagttattca aacacgagat acattaagtg
    19861 ccgaaacaga aggtagtatt attaattatg gaaatactcc aaatacaata aagactatta
    19921 gtattacaag ggatggagtt gatgtttctt ataattatga aaatattaaa aaatctgcag
    19981 gaactttgac tattacaaaa agacctatta ctgtaaagag taaagatgga atttgaacat
    20041 atgatggatc agaacatttt aaagacgaag tggaaattat tcaaggttca tgagttgaag
    20101 gcgaagatgc ttcgtattct gcatttgcaa aagtaaaaga cttcacacct caagctgaaa
    20161 acaatacata tactatgtct ccaaagggag aaactaaatt tggcaattat gaaattactc
    20221 aaaagtatgg aatgctttca attactccaa gaaatattac gcttacaagt ggcactgcta
    20281 caatgactta taatggtgaa gcccaaaaat atgaaaatgt caatgtaaca acaggatcat
    20341 gagttagtgg cgaaggtgca agctacactt atacatcttc tgttacattt gtctctgata
    20401 gcgaagttga taatacgttt aactactcat taaatgatgt gacaaaagct gcaaactata
    20461 acatcacaaa aaagtatgga aaaatttcta tcaaaaagat tgaaacacca ttaattgtta
    20521 cagctaattc aagttcgcat acttatgatg gaaacgctta cactgactct ggttttactt
    20581 attcagataa tattgttaaa ccgcttgata gtgtttctgc tttagtttca ggtacaatta
    20641 ctgatgttgg aagtgcggat aattttgtaa cttctgtaag agttttaaga gataatgatg
    20701 atatatctga taattataca attggagaat acgttaaagg taccttaaca gtaacaccta
    20761 gagatattac aattacaagc ggcactaaat caatgacata taacggttat gcacaaaagt
    20821 atgaaagtgc tagcgttact tcaggatctt ttgtttctgg tcaaggggca acatacagtg
    20881 gttttgcttc ggttacattt gtgtctgaag gtgaagttga caatgaattt tcttactcgc
    20941 taaattctaa tactaaagaa caaaactata acattacgca ggtaaatgga aaaatttcaa
    21001 ttaaaaagat aaccacacct ttaattgtta cagcaaattc aaattctaaa gactatgatg
    21061 gtacacccct tactgattct ggctttacat gaactagtgg cattattaaa ggaagcgatg
    21121 ctgttactgc aactgtaact ggttcacaaa ccaatgctgg tagttgtgat aatatgatct
    21181 caaatgttac agtcatgcgc gataatgtag atgttagttc aaactacaca attggaacac
    21241 cggtaaatgg tacattaact gtaaataaag ctacaccaaa cattgtttga gatggagtta
    21301 aaaaagatgg ttactataat ttaaggaagt caagtagtgc tactccaaca tatacattat
    21361 tatttggaag agagggcatt tctgtttcta caaacaattt tgatcaagat gcttctttaa
    21421 atataagcgg gaaagattta ccaaaaggtg ttagtgttaa aaacaatgat agaatttata
    21481 ttgattcaac tgcaacagga tctttatttg tcttaaaaat tgttgcaact tctggagaaa
    21541 cagaaaattt tgtccctggc agccaagaag ttgaaattaa aattgcaatt acttcgttgt
    21601 actatggaat tagtaacaaa acattgtatt tatctgcttt gccaacggaa aatgcaacaa
    21661 ctgaaaattc agcagcactt ggttgttatg atccttcgac aaaccttgct tgatataaca
    21721 gcaggaattc tttagaatat attcgtattg aatctgttga agatactaaa atcacaccaa
    21781 cttctctata tcaatgattt agtagtacga gcctttgacg tatatatggg tgcgaaaata
    21841 ttgatacttc acaagttaca aatatgcgtg gtgtttttga aaactctgag aatatttatg
    21901 aattagacat tagttcttga gatacttcgt cagttacaaa tatgaataga atgttttatt
    21961 ggtgtaaagg acttcagtat tatgactttt caaattttaa tactgaaaag gttacaaata
    22021 tggctgaaat gtttagtacc tgttgacata taaattctga ctttagtttt cttaaaactg
    22081 gtgctgttac tactatgtat cgcatgtttt ataactgtaa aaatattgaa aatattgatt
    22141 tatcttcttt tgatacaagt aatgttacaa atatggaagg aatgttttcc gattgttata
    22201 acttaaaaaa tattaatatc tcttcattta atactagtaa agtaaccaca atggcttcaa
    22261 tgtttgccaa tagtgaagag gggttagaag tgcttgacct ttcaagcttt gatacaagtt
    22321 ctgttgtgaa tatgactgct atgttttctg gatgtaagta cctccgaact atttttgttt
    22381 cctcagactt tgtgacaact aaaatagaag gtgcttatgg tgattctatg tttgatcgtt
    22441 gcgatgagct tattggtgga cgcggaacat caccaggacc tactgattat acttatgctt
    22501 gaattgatgg tcgtgataat actgagggat atttcagttc gaaactttac tgaggggttt
    22561 ctaacgatgg agaaatttta tatatttcac ctgacgattc tgaaatgaca ccaaaaatgt
    22621 cagtttccgc accagtaggc ggtgagattc catgaatcaa taatatctca agttataaaa
    22681 aagcttatat attagaaggt aataacaatg ccaagctttc agcgtattca atgaaatcat
    22741 gatttgaaaa tgcagaaatt cttgaagaaa ttcatggttt agataaccta aacacagcgt
    22801 atttgagttc gcttcaactt actttttcgg gttgtgctaa tattgaaaac ttagatgttt
    22861 caacctttga tacatcaaac gttacaacta taaatgggac ctttaaaggg tgttcttccc
    22921 taaccttttt agacttgtct aattggaatg tttcaaaagt tactgcagtt gctagtcttt
    22981 ttgctaattg tactaattta gtatcttttg aaaaacctaa aaatagtgtt gctaaaatta
    23041 cttctaacgc tactgctgta tttggaggtt gtgaatcttt gaaaaatatt gaacttgatt
    23101 gactagatct ttgcaaatct agttcagtac aaaatttctt tataaactgc aaattagtag
    23161 aagtcttgga cttgtcctct tttgattgtt ctaacgtgaa attaacaatt ggtatgttta
    23221 gtggttgctc taatttacgt tcgattattg ttggtgaaga tttccatttt tcaagcgttc
    23281 taaattcagc tttgtcaaag aatatgtttt ttggttgcaa taacttaatt ggaggtagag
    23341 gctttgcttt tgacgaatca ggtccggttg attttactta tgcatatatt gatttaccag
    23401 gtaagcctgg gtattttact ggtccttcat ctctttattg aggtgtatca aacttaagta
    23461 atgaagattg taaattatat ttgagttcta ctatcatgcc tcagactcct aattctggtg
    23521 gtaaggcttt agtttctgct tcggctgatg atattccttg agctaaatat ttaacaaata
    23581 ttagagaagt tgaaattatt agttatgaaa gcaatgataa aattgcccca aattcgatgg
    23641 cttattggtt ctgtaacatg aattatgttt tgacaattaa gggttttgat aaaattgatt
    23701 actcttctgt aacagatatg tctcatgcct ttgaaaattg ctgaggcctt actacccttg
    23761 atttaacatg tgttaattca agcaatgtta caaacatgga aaaaatgttc tactgttgtt
    23821 attctattaa caaaatttat gcaagtgacg attttgagac ttcgcaagtt actaatagtt
    23881 ctgatatgtt tactggttcg tctaatatta aaggtgaagg tggtacttct tataacggtt
    23941 cgtatattga taaaacatga gctcgcattg atggtaaaga tgggcaaaaa ggatacttct
    24001 gaggctgttc attatattat tcaataattg gaagtagtga tttaaccttg actttatcct
    24061 ctattaaaat tgatgattcc tttgagcagt atgaagatgc tttgtataat gaaacagcta
    24121 atttgccttg aagtggtcgt gctggtaaaa taagttcagt taatattgtt atgccagatt
    24181 tttctacggc aaaaattaaa ccatataaaa tgggaagatg atttagtgaa ttgggacagt
    24241 ctgttggttt agtacaattt acaggattag aaaatatcga tacgtctaat gtaacttata
    24301 tggctgaatt gtttaaagga acggttctcc cagaaaaatt tatcttccct gcctgagaca
    24361 ctttaaaagt tgaaaatatg tctgaaatgt ttagctatta tggatatgat gatgattctc
    24421 aaaacagaat tttagactta acaaacatgg acacttcttc cttgaaatac gctgatgata
    24481 tgtttaataa atgtacatct cttcaaacaa tttttgtttc tgataaattt gttacaacaa
    24541 atatagctac cagtaaatat atgtttgaaa attgtctttc tatttatgga ggaaaaggaa
    24601 ccatatatga tagtagttat gtggatgcaa actatgcaca ctttgatgaa ggctacatta
    24661 gtgaaaatcc gggatacttt acttctttaa cagaccagtt gttgtattgg ggtttatctg
    24721 atgataaaac aaagttatat ctttcgtcag aatactctga tactacgagc aatatgggcc
    24781 tacgggcttt gtatgcaagt aaaaattcat atgttgaatg actctcttca cttactaatg
    24841 ttaatgaagt atttgtatta gactctccag caggcaaaaa aatcaagaca caatcagttg
    24901 ttggttgatt tgctaatgct gcacaattaa caaaagtcaa tggtttagaa aacttggata
    24961 catctaaagc gtccaatttt cttggtttgt tccagaagtg tacaagttta gtttcttgtg
    25021 atttgtcaat gatagataca agtggagcag ttaatctaca aggcatcttt aatggttgtt
    25081 caaacctaaa agaagttaat gtctctactt gaaatacatt taattgtgat agatttacag
    25141 caatgtttaa tggttgttcc gctctagaag tactagattt atcttcgttt gtaactaata
    25201 aagatttaag cacattttct gtttctatgt ttgctggttg tggcaatctg aaaactattt
    25261 atgttaatga gaatttttgc gaatttaaaa cttcaatgca aatgtttaag agttgcttat
    25321 ctcttgtagg tggaaaagga acaaaatttg atccaaacca tattgatagt gactatgcac
    25381 gtattgatca aggagcattt aatcctggat attttacgtc taaggaccca gccgcagtga
    25441 caatgccaaa tgatgggctt ttgttgaaca atccaaatgc tcaagcacct gaaacgcaag
    25501 aagcagatcc tcaagctcct gtcaatgatc ctgaagttaa cccaacacct acagagagcg
    25561 gtggtgaagg cgttcctgca ggtggagaag ccgctgcatt agttggtgtt cctctagggt
    25621 tggcggctct cggcttccgc cgtcgtatta gcggcaatca tgtaaaaatt aaataacagg
    25681 ctcgccccgc cttcgttaat taactaatat gtgtccctga cttaaaactc agggacattt
    25741 ttttcttgac taaaaaggtg taattcatgc caggcaataa ttacaccttt tggtgcattt
    25801 catgccaggc aataattaca ccttttatat gaaaataata gtggtataat acggggttgc
    25861 acaaaagaag tttaaacgta gtaagaatgg agatgaaatg gcaaaacaag tggacacatc
    25921 tgtgccaact aatcctattc caaatgaccc aggtactact gcgcccgttc gaccttcggg
    25981 ggccagcggt cgcgacttaa cacctgatgg gggcactttg gaaagtggat tggtttctgg
    26041 cgaagacgtc gcagccagca gccttactcg cgggacatcc cgaaacattt cgatggatgg
    26101 tgccggcgga gggctcaacc ttccgcaggt aaatgttgcc gcggaaaatg caaatgctgc
    26161 acgtccagta atcacgttta gaaatgttac aaaagtttat gctgctcaac ctacgaagcc
    26221 ggcgctcaag gatatcgacc ttcaaatttt tgctggcgaa tttgtgtttt tggttgggca
    26281 ttctggaagt ggtaaatcga cgttcattcg acttatcact cgcgaactta aggcgactcg
    26341 cggccaactt ctgattgcgg atgaagacct tggcacaatg aaaaattggc gtgtgcctta
    26401 tcttcgtcgt aatattggtt gcgttttcca ggactttaag ctgcttccaa acaaaacggt
    26461 ttttgaaaac gttgcgtttg cgcttgaggt tattggaaaa agccgccacg ttgttaaaac
    26521 tcaggtgccc gaggtgcttc gcctcgtagg ccttggtgag aagctgcaaa aatatcctga
    26581 ccagctttct ggtggtgagc agcaacgtgt ttcaattgcg cgtgctattg taaaccgtcc
    26641 gccaatgcta atttgcgatg agccgaccgg gaacctcgac cctcaaactt cgcgaggcat
    26701 tatggacttg ctcgagcgca ttaacaaaac tggtacaacc gtgcttgttg caacccatga
    26761 tcgcgaaatg gtaaacagca tgagacgccg cgttgtagcc cttgaccacg gtgttctcac
    26821 tcgagatcaa gacagggggg tgtacggttc aaatgtctag tttgtattac tttttacgtg
    26881 aagcgctgat tggttttaag cgcaatttgt cgaccgcaat tggttcgata atcacaattt
    26941 tcctttcgct tttgatgatt ggcttgttcc ttgttggcgg cttgattgtc aacaacattg
    27001 tttcgaacgt cgaaagcgaa gttatgatca cctgctacgt tgcagatggc acgcaggaag
    27061 ccgatgttaa aaagctcatg aatgaagcca aagatatcga cgaagttgaa gacgtaacct
    27121 tcaccacgaa ggaccaggct cttgagaagt ttaggaattc catgagttca agtcctgaaa
    27181 ttgttgatca gcttgacggg caaaatccac ttcctgccag cgttaatgtt gagcttaaaa
    27241 accctcaaga cgtatcgaag gtagctgagc aaattaataa atcaaacaat tttaagaaga
    27301 ttgctgaaaa ccaggccaag cctgaagatt cgcttaaata cggtcaaaag actgtcgacc
    27361 agctcttcgc gctaacaaat gttttgcgta ttgttggaat ttgtatcatt gctttgctca
    27421 ttttcatcgc gatgattttt atcaacaaca caattcgcct tgcaattttg gcgcgccgca
    27481 aagagattgc aattatgcgc ttggttggtg cttcgaacgg attcattcgc ggaccatttt
    27541 tcatggagag cattttgcat tcgttgattg ggtccttagc tgctattttg attatggaag
    27601 tgttgcgaag cttcgcgctg cctgcaatgg ttaatgctct tgcttggctg cctgttgact
    27661 tgtcgcttgc aaccttccta attatttatg gaattttgct tgtagccggt atcatcattg
    27721 gacttatagg ttccgcgctc gcaatgcgcc gctacctcag cgtctaacca aactcgattt
    27781 ccccggggcc agtactcgat tgtctccggg gttttactcg atttccccgg ggccagtact
    27841 cgattttatt tattattaag tgaaaaatgt gtagaatgcg tctcttatgc gttttttatt
    27901 tcaaattttt tataattaat taataataaa aaaatgaact gggaaaacaa aaatatactt
    27961 ttttaaagta atgaaaaaac acataaagga cgaaatctgc acatctatat aacaaaattt
    28021 gtgaataaaa aaataaaaac acataaagga cgaaatctat acaagattta aaagaagagt
    28081 gggccgataa gccgggttct gtcgtgtgag gaccatttat cttgtctttg cattgctgcg
    28141 aagctctagc gcgcaacccg aatgcagtgc gggcaacacc atagcattcc tatttgcgtt
    28201 tgctccggat ggggtttacc aagctacttc gtctccgaag tactggtgcg ctcttaccgc
    28261 accgtttcag cttttctctt aacgccaagg cgccagtgag agttttcttt tctgcggcac
    28321 tttccgtcgg gtcgccccgc ccagctgtta gctggcatcc tgccctttgg agcccggact
    28381 ttcctcgtta aaaacgcagt cctcatggcc cactctcaaa gccgaggtat tataacataa
    28441 ccagaaatta ttgtaaaatc gagtaaatgc cacgagtaaa tcgagtcctg gccccgggga
    28501 aatcgagtaa atgccacgag taaatcgagt cctggccccg gggaaatgaa aaagtggtat
    28561 aatcgggcgt gctatatttt tattcaagga gggaaaagtg gcagttgaaa aggttgaaaa
    28621 gatgccatta gcaggtgcga caaacggcga acttgctgta gcagaaatca ccaaagccct
    28681 caacaacaga atggctgcgg gttctcttgg gtcttgcccg gttgaattca caaaagcttt
    28741 tgttgaggca tgtggttctc aaagctgcgg aaagtgtaca ccttgccgta tcggcttgaa
    28801 gcaaatggca aaaattcttg gtgaaatttt ggaaggcgag gcttgcgaat gcaagttaaa
    28861 ggtgcttccg gatttggctt cggcaattta cgaaagcgcg gattgcgcga ttggcttcga
    28921 agctggccgc caggttttgg aaagtttgaa aaagaacgat gatgactata agtatcattt
    28981 gcatcatgac ggatcttgca tgtatgagaa gcgtgcccag gttccatgcg ttgagggttg
    29041 ccctgctcat gttgacattc cgggttacat cgcttgcatt caggctggta gatacacgga
    29101 tgcggtccga gtaattcgca aggacaatcc gcttccgctt gcatgcggtc taatttgtga
    29161 gcacccttgc gagcttagct gccgaagaaa tatcgtcgac gatgcaatta atatccgtgg
    29221 tctcaagcgc tacgctgttg atcatgctgg aacttatact cagcgtaagt atcctgctac
    29281 tcgcaagaaa gttgcggtaa ttggtggcgg accttcgggc ttgaccgcgg cgtattacct
    29341 gaccattatg ggtcacgacg tcacaatttt tgagcagcgc tctcaccttg gtggcatgat
    29401 gcgttacgga attcctgcct atcgtttccc tcgaaaagat atggataaag aaattaattg
    29461 gctgctcaag cagggcatta aggttaagta taacaccaat gtcccgcgcg atgtcagcgt
    29521 ggccgagctt cgcaaaaagt tcgacgccac ttatattgca attggtgcgc acagcggaaa
    29581 ttcgcttcgt attcctggag aaaacgccga aggcgtgctt tcggccgttc aacttcttgg
    29641 cgccattggc gatggcaaaa agcttagctt taagggcgaa gacgtggtgg ttgttggtgg
    29701 cggaaatgtt gcaatggatt gtgcacgaag cgctgttcgc ctgggtgcga agagcgtcac
    29761 aattgcttat aggcgccgca aggatgacgt aacttgccaa atggaagagc ttgatggtgc
    29821 tctcgaagag ggctgccact tgcttgagct tcatgctcct gttgaagtta tgaccaaaaa
    29881 tgacaaggtc acgggcatta aattgcagcc tcaagtttct ggtccggttg accgaggccg
    29941 tccgaagcca atgccagcca accaaaagcc agttgttgtt aaagctgata agattttggt
    30001 tgcaattggt caggctatcg attcggcgaa gttcgaaaaa gccgggttgg gcgtgaaccg
    30061 ccgtcgcttc gttgccgata aattcaccga agcgctcgac aaaaaaggca atgttatcaa
    30121 gggcgttttt gttggaggcg attgcgaaag tggcccagca actgttatta aagctgttgg
    30181 agctggtaaa gttgctgctc gaaacatcga taattacctg ggctttgacc acaagatcac
    30241 acttgaggtt aaaatccctg aagcccgtgt tggcgataaa aaagctgccg gccgcgtaaa
    30301 gcttatggag cgaagcgccg ccgagcgtaa agacgatttc aatctaatgg aaaaaccaat
    30361 gagcgatcag gaatgtttgc ttgaatgcaa cagatgttta aggtgtgatc atagcggcat
    30421 gggctctttc cgtggaggaa gggagttatc atgataaatt ttaagattaa tggtcagaag
    30481 ttaaaggctt ctgcaggtac tacaattctt caagctgctc gcaaaaacaa gattgacatc
    30541 aagactcttt gcgaatatga aaagctaaat tgcattggcg cttgccgtct ttgcatggtc
    30601 gaggtcaagg gcttcgatcg cctcgttgct gcttgcaaca ctcttgtcga agaaggtatg
    30661 gacattttga ccgacacgcc gaagactcgt cgcgcagttc agaccaacct cgagctcatt
    30721 ttgtccgagc acaatacaga ttgcacgaag tgtgttcgca acggaaattg taagttgcag
    30781 caacttgctg aagaccacaa cattcataag aatatttttg gcgaagaatt caaaaaggtc
    30841 agctggcctc gttctttccc acttcaacgt gatgaaagca agtgcgttaa atgtatgcga
    30901 tgcatccaag tttgcgacaa tattactggc gcaaaagttt gggaacttaa aaatcatggg
    30961 acgcgcacac gtgttggcgt ggcttcggat ctaaagattg atgctaccaa gtgcgttctt
    31021 tgcggtcagt gcattactca ctgtcctgtt ggtgctctta gcgaacgcga cgacatccac
    31081 cagcttcaac gtgctttgga tgatcctgag atcacaactg ttgttcaagt tgctcctgct
    31141 gttcgcactg catgggccga agaaatgggc ctttcgcgta agcaagcaac aatgcaacat
    31201 atggttgcgg cgcttaaagc aatcgggttt gactatgtct tcgatacaga ttttgctgct
    31261 gaccttacaa ttatggagga aggcaccgaa tttttgactc gccttaagca caagaaaaat
    31321 tacgcgtggc caatgtttac atcttgttgc cctggctgga ttcgtcatat gaagcacaac
    31381 aggcctaatc aaattaagca actctcaact gcgaagagtc cgcagcaaat gtttggtgca
    31441 gcttgcaagg agcttttcaa gactcaatat aaaattgcac ctgaaaaggt atattgtgtt
    31501 tcaatcatgc cttgtttggc gaagaaatat gaatgcgacg ttcctgagct caacgatgtt
    31561 aagcgtggtg ttaaggatgt cgatgcttcg cttactgttc gcgaattcca gcgcatgatg
    31621 aagatgcaca acattcatcc ggccgactta aagcctgttc ctttcgacaa tcctcttggc
    31681 gaagctacag gtgcggcgca catcttcgga gctaccggtg gtgttatgga agccgctttg
    31741 cgtaccgcgt attttgttgt taacaagaag aatccgccgg ccgatttgtt taagagcgtt
    31801 cgtggtatgg atggctggaa ggaagcaact ttcaaacttg ctggaacgcc tgttcgagct
    31861 gctgttgtta gcggtcttgt taatactgaa aagctgctta ccgctcttga taacggcgag
    31921 gttgagtacg actttgttga ggttatgact tgccctggtg gttgtgccgg tggtggcggt
    31981 cagcctatta aagaaggtgt tgagatggcc aaagttcgtg gtgatcattt gtacttctta
    32041 gataagaagt cgaagagccg ctttagccat gagaataaat ctgtccaaga aatttacaaa
    32101 cagattttca agaagcctct gtctgaaaaa tcccacaagc ttctacacac cgatcaaagt
    32161 aagtggtcca tctaactcga ttttaaaaag gtgtaaatat tgcctggcaa tttttacacc
    32221 ttttttcttg caattgcttt gtatttgtgc gagaatacgt attccgcaaa cgtggtcgat
    32281 tggcgcagcg ggagcgcagg tgccttacaa gcacaaggtc gcaggttcga atcctgcatc
    32341 gaccaccact tttgtgctcg gcctgacggc ctgcgcacta agctttcgct ccgaaataat
    32401 tctatctatt atctttaccc ttgttttttg actcgcgtac aaagtacgcg tcgtcaaaaa
    32461 ggcgggcaaa tctaatagat ataattattt ctcgctgtct tagccaaaag ctattgatgg
    32521 caattttttt tatggccttt tttggggacg ggggcagcgg gagtttattc ttttttaata
    32581 attagtagaa taaaattttt aaaattattt ttgtttatta aaaagcaata aataattttt
    32641 atttttaaaa aagtgtttaa gaatgtttat ttctggttaa gagttcatgt cttgtttttt
    32701 cattgtgtat tttgtaggat tttcgcaagc ttgagtgact gctgaaaaaa ggttgcttct
    32761 cgcagtaatt tgtgctacta tatccgggct atcctgcttc gggta
//
LOCUS       DBNCDC010000003        28166 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_346_length_28166_cov_8.176479, whole genome shotgun sequence.
ACCESSION   DBNCDC010000003 DBNCDC010000000
VERSION     DBNCDC010000003.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 28166)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 28166)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28166
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_346_length_28166_cov_8.176479"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(26..346)
                     /gene="rnpA"
                     /locus_tag="AC04VQ_00535"
                     /pseudo
     CDS             complement(26..346)
                     /gene="rnpA"
                     /locus_tag="AC04VQ_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013022.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
     gene            complement(350..484)
                     /gene="rpmH"
                     /locus_tag="AC04VQ_00540"
     CDS             complement(350..484)
                     /gene="rpmH"
                     /locus_tag="AC04VQ_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIO9552754.1"
                     /translation="MKRTYQPNKRKRAKVHGFRSRMSTKGGRGVLASRRAKNRKKVSL
                     "
     gene            complement(576..1661)
                     /locus_tag="AC04VQ_00545"
     CDS             complement(576..1661)
                     /locus_tag="AC04VQ_00545"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIO9552755.1"
                     /translation="MKKDFENRIIKLRKILEERNVDAILIYKNRDVLYTTGFYDKENN
                     SVAIISKDKLIFMTDGRFVNQFNEQVEGFDLILFENIFNKFEKVNEVLEENNINKYLI
                     NFEAISYKDALSLDANIENIVDSTELITNFRRIKTEDEIKLTKKACDIAEQSLLYTLD
                     QIKEGMTELEIQNILNSEMSRRGSSRVAFPTIVVSGPDNGASPHGVPTERKVQQGDFI
                     TIDFGATYQNYTSDITRTIAIGKVSDEMMEIYNQVLKAKTECVSMLKPGESTNKIHKH
                     ADSVLKEIGYDLVHGLGHGIGLENHELPKLTISTDYQLQVNDIHTIEPGIYAPGICGV
                     RIEDDYLITEDGYECLTPNITNELITL"
     gene            complement(1875..3305)
                     /gene="dnaA"
                     /locus_tag="AC04VQ_00550"
     CDS             complement(1875..3305)
                     /gene="dnaA"
                     /locus_tag="AC04VQ_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIO9552756.1"
                     /translation="MVKKVEELDVGGAQKVDAFLPQLKPQAISNDFLMLTTNNSFIKF
                     WVESHILGLIKQALSSLYGTEFNVQIEVDQTEDADTEYTSESYIAANLVSSNTKLDTD
                     PSNVFTSQNSEEEVVTATPNFDMNLTNLLTFENFVIGSSNNMAFQLARSVAETPGKPN
                     LNPLFIYGKSGVGKTHLLHAIKNYININNPAINVVYIDATAFVNEYTAASAAHDVDKS
                     SFVNFNKKYESAQVLLIDDLQLLQDKVATLNTFFTLFNNITNLGRQIVISADRAPKNI
                     DVDERYQSRFNNGMSCEITSPEVEVKLGIIKSYIDDFKKEEPEIENYISDDVVNFIAQ
                     NSSSNIRELKSAVTKVLYEIKYNHENIPVDKVKEILEEHFSKNIKKRVTVENIQKVCE
                     EYYGVSHNALVGKKKDRNIAFARQISMYLCYKMLEISVTAIGKSFNRDHSTVLHSIKV
                     IEEKLKNNIEISGEIELLEKRVLEKD"
     gene            4070..4327
                     /locus_tag="AC04VQ_00555"
     CDS             4070..4327
                     /locus_tag="AC04VQ_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552757.1"
                     /translation="MGKEMACLVLVIVLCFSYGSIRLALNPKSPDSTRVASVLNFYGT
                     KDFIPTEEGIKLYQDEHVNESLVYMEQDVINAKKEGASIIM"
     gene            4361..4699
                     /locus_tag="AC04VQ_00560"
     CDS             4361..4699
                     /locus_tag="AC04VQ_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIO9552758.1"
                     /translation="MDHFLSKASQFAKENEIALLIPIDILQERGEKRLNQAIFINKDG
                     NVDFRYDKVNLVPIVETFDYKEGEPLLCHSTVDDFKVCAAICYDALNPQFIRAHMHED
                     TDILLVPALE"
     gene            4733..5011
                     /locus_tag="AC04VQ_00565"
     CDS             4733..5011
                     /locus_tag="AC04VQ_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552759.1"
                     /translation="MFRAVEHGVSYIRNTGAGIAFACDQLGRPVSYYNTLGDNPSILY
                     IDLPTDHKVTIYQYIGDTIVYLSILYFAFIVIYVIVKNKKKKSQKKSK"
     gene            complement(5013..5828)
                     /locus_tag="AC04VQ_00570"
     CDS             complement(5013..5828)
                     /locus_tag="AC04VQ_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015778957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIO9552760.1"
                     /translation="MAETETKHRKTSYSSLQREDFSVVRTPARVAVYDDLKAPPRVID
                     VSPDITNNFIENLASTIYDEANKKGSKIPYTVIREISENFIHARFTEIVVSILDNGNT
                     IRFTDQGPGFTDTENAQLPGFTSATEGMKQYIRGVGSGLPTVKDYLNYSDGNLKIENN
                     LENGAVVTISMLENLSSSSAFSDFDKKQSDTKYVEKMTKLSPTLSAREREVLKLFLTE
                     GDLGVTQVKDLLNIPLSSTHKILTSLEQSGLVQRVGNKRRILSDQGFAVASIL"
     gene            7094..8266
                     /locus_tag="AC04VQ_00575"
                     /pseudo
     CDS             7094..8266
                     /locus_tag="AC04VQ_00575"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            8269..8871
                     /locus_tag="AC04VQ_00580"
                     /pseudo
     CDS             8269..8871
                     /locus_tag="AC04VQ_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013468.8"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
     gene            9087..9485
                     /locus_tag="AC04VQ_00585"
     CDS             9087..9485
                     /locus_tag="AC04VQ_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIO9552761.1"
                     /translation="MVRKIRKISQTGYYHVMSRGNNKQIIFRDESDRLKYLSCIKDAL
                     KKHNLKLICYCLMSNHTHLVIYDKENQLSLFMLTLNSKYASYFNKKYERVGYLFQDRF
                     RSECIGDERQLLAAYRYVLNNPYKAGLCRP"
     gene            complement(9799..10959)
                     /locus_tag="AC04VQ_00590"
     CDS             complement(9799..10959)
                     /locus_tag="AC04VQ_00590"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005534 - galactose binding [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HIO9552762.1"
                     /translation="MGKNFKAFAPGRTEIAGNHVDHQHGIAVTGSLKQGISADVELRD
                     DKIIHINSEGFEPFDVDIDKVAEAMAPDPSKFNTTEALVAGVAHKFIEAGVPISGFTA
                     NCSSTIGAGMGLSSSAAFELLIAVILNDAYASGKFSKMELAQIGHFAEVEYYGKPCGL
                     LDETAISYGGIIKINFANPEVLDVKPIDFSFERAGLGAVLVDSGAEHGDISHMYAAIP
                     GDMKKVAELCGVEFLNETNIDTLSKNLMNAEKVLGDKAANRGINYFHEINLTKERIKA
                     MEAGDGKLFMQLHGLSGSSSAQFLQNVTVSEEDQQATLCQAICELCLDQACEGDYTNR
                     GASRIHGGGFGGCMQVFLPIEQVSKFCTLVNEIYGREAATEIQFSDTGATTL"
     gene            11112..12236
                     /locus_tag="AC04VQ_00595"
     CDS             11112..12236
                     /locus_tag="AC04VQ_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIO9552763.1"
                     /translation="MGIFNRKSEGGRKKPTTGFSELNRRTYEASPTGRTGQIDPTNTV
                     GYAGNPNAKKQSRRRGADLGFKPQESLSDYSRRGQQQKYAAARKAQDKKKRKKKIIII
                     VCSILGALVAAGAIAFGIISCNLNAGMGNIGLTPTSLDKPFYMVLMGVDSSDVRKAES
                     GGDDSDYRSDSMILARVAANEKKVTLMSIHRDIEMDMGGEYGVQKINASHSIGGPELV
                     IKNVSKLAGVPINHYAEINFDGFTAAVNDLGGVDVNVPIEINDPEAGGYVPAGNQHLN
                     GQEALILARSRHAYDAYGDGDKFRAANQRMLLSAIAQKALDSDIMTIAQTVMDMSGYI
                     KTDLAINDMIGLAQLMKGLNPAKDVYTAMTPTQGVYKNGG"
     gene            12270..12641
                     /locus_tag="AC04VQ_00600"
     CDS             12270..12641
                     /locus_tag="AC04VQ_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HIO9552764.1"
                     /translation="MMKRMDAGLPPNEEPYIDPATGTVMSTSGSGDWEGTVSVKNGSG
                     VDGAAASASLKIIALGFTATTGNADKDNYSKTLIIYKDDDKEDAAEAIADKLGCGRVQ
                     QNDGDYDFDTDILVIIGLDFE"
     gene            12726..13901
                     /locus_tag="AC04VQ_00605"
     CDS             12726..13901
                     /locus_tag="AC04VQ_00605"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent malic enzyme"
                     /protein_id="HIO9552765.1"
                     /translation="MDYAKESLKKHAEWKGKISTELKCPLENATDLSLAYTPGVAEPC
                     LAIKDDIEQSYNLTMRGNLCAVITDGSAVLGLGNIGPEAGMPVMEGKCALFKKFAGVD
                     AIPICVKSQDPDEVAHVVHMISGSFGGVNLEDISAPRCFEIERKCKELCDIPIFHDDQ
                     HGTAIVVLAGLINALKVVDKKAEDCKVVTCGAGAAATAIVKLLLSYGFKNVTMCDSKG
                     VIHKDRDDLNWIKKEMAEVTNPEGITGSLADAMVGADIFIGSSAPNTVSKDMVASMAE
                     KSIVYACANPTPEIFPDDAKEAGAFVMATGRSDFPNQVNNVLAFPGIFRGTFDVHASD
                     INEEMKMAAAKAIAGMVSEDELGPECIIPSPFAEGVGEVVAKAVAEAAIASGVAKKK"
     gene            13915..15096
                     /locus_tag="AC04VQ_00610"
     CDS             13915..15096
                     /locus_tag="AC04VQ_00610"
                     /inference="COORDINATES: protein motif:HMM:NF014098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HIO9552766.1"
                     /translation="MAATISRGVRAPIIREGDDIVQITVDSVLAACKEDGFELGDKDV
                     VCVTESVVARAAKNYCSTDDIAADVKAKLGGETVGIVFPIFSRNRFAICLRGIARGAK
                     KVVVQMGYPSDEVGNHLVTEDQIDDSGVNPYSDVLTADEFHAKFGDIKHEFTGVDYLD
                     YYKELIEDEGAEAEVILANRPEAILKFTKNVLVCDTHSRDRNARRIKKAGGETVLTMA
                     DIMNASVNGSGYNSKYGLLGSNKATESTVKLFPENAQEVAEAISNKLSEITGKQIEAM
                     VYGDGAFKDPVGKIWELADPVVSPGYTKGLTGLPNELKLKYLADNDFADLSGEELQAA
                     VKKAISEKEGDLKGNMASQGTTPRQITDLIGSLCDLTSGSGDKGTPIIVVQNYFKNYS
                     M"
     gene            complement(15500..16342)
                     /locus_tag="AC04VQ_00615"
     CDS             complement(15500..16342)
                     /locus_tag="AC04VQ_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552767.1"
                     /translation="MQKFFIYITSALEFYKNARTNLDLDLENSDPTKNTLTLLPCSKQ
                     PSSDLRTTKNSVIIEDLPYCGNAPYHLLTKNKNYNRHLKNIKFHYSNITYLRNSFLKY
                     NDNIYIPTPPLLFYQLATIMPFEQLLQTGLEMCGTYSISESAANGYVYNIPPLTTALK
                     IKTYITNLNKRHKNLPGIRKALQASSLLIDNCASPQESNLYIKLCAPRNMGGYEISNI
                     KANCQIKLSEQAREILKHPTIKPDLCNKKTKIAIEYDSNEFHDNTGQNTKDKLRADAL
                     QHDG"
     gene            16415..17125
                     /locus_tag="AC04VQ_00620"
     CDS             16415..17125
                     /locus_tag="AC04VQ_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIO9552768.1"
                     /translation="MEDRIYIAIDLKSFYASVECVQRNIDPLDSYLVVADKSHTEKTI
                     CLAVTPALKDYGISGRARLFEVIEAVDEINRIRGRRLLELYDDVDATGRHGAVSKKFT
                     KKCAFDAELKSHPEYELDYIVAPPQMAKYIQVSTQIYEIYLRYVAPEDIHVYSIDEVF
                     IDATPYLMMHDCTAEELARRMIADVLAETGITATVGIGTNMYLCKIAMDVFAKRMEPD
                     KNGARIAFLDEQGYRQHM"
     gene            17153..17296
                     /locus_tag="AC04VQ_00625"
     CDS             17153..17296
                     /locus_tag="AC04VQ_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552769.1"
                     /translation="MIGAGYTRRLEAYGMHTMGDIARCSIENEDLLYEIFGINAELLI
                     DHA"
     gene            17372..17620
                     /locus_tag="AC04VQ_00630"
     CDS             17372..17620
                     /locus_tag="AC04VQ_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552770.1"
                     /translation="MHRPYKNDEGRLVLREMADALSQELVSKNLVTKKLVIHVGYDIK
                     NIMDIEKGIVNAYAGEISTDHYGRKVPKYARKTINLPR"
     gene            17642..17986
                     /locus_tag="AC04VQ_00635"
     CDS             17642..17986
                     /locus_tag="AC04VQ_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552771.1"
                     /translation="MDAVADAFDQVVDKQLLIRRFNVTAEAVLPKTQVDEAAAAAPPK
                     VTQMNIFTNYELEEKLKAAEDEKLERELELQRVMLAVKEKFGKNAILRGMNLQEGATM
                     KERNAQIGGHKA"
     gene            complement(18095..18268)
                     /locus_tag="AC04VQ_00640"
     CDS             complement(18095..18268)
                     /locus_tag="AC04VQ_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552772.1"
                     /translation="MENSSEKYKDIIDMPRHEPDEEKHPRMPRYNRAAQFAPFAALTG
                     FEESIDKASKNKK"
     gene            18425..20281
                     /gene="typA"
                     /locus_tag="AC04VQ_00645"
     CDS             18425..20281
                     /gene="typA"
                     /locus_tag="AC04VQ_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIO9552773.1"
                     /translation="MSVKREDIRNVAIIAHVDHGKTTLVDCLLKATDVFRQNQKVDER
                     VLDSNDQERERGITILSKNVAINYKNKKINIIDTPGHADFGGEVERVLNMADAALLIV
                     DAFEGPMPQTRFVLKHALSQGMRIILVVNKIDRDGARPDAVVDEVFDLMVDLNATDAQ
                     LDFPIIYASAAQGYARAELTDTNTDMFPLLDMIINEVPAPEVEAEEPCALQISTVDHS
                     SYQGRIGVGRLGFGTLKKGQQVLAVNNHGKKYKTQIKQLFVFENLGKNEVTEAAAGDI
                     VAVVGVDDADIGDVIVDVPGDDAAAIDEAAARAANLFAPIEVEEPTMAVIFEPSTSPL
                     VGQDGEIVGGRQLKERLLREKESNISMRITEVADSTAMEVAGRGVLHLSVLMETMRRE
                     GYEFQVGRPRVLVKTDEAGRTLEPIEEAVVDVDNEYSGKVIEVFGNCGGEMTEMNAKA
                     DQTHLVFKIPSRGTMGLATKLLNVSHGSATLFHHFAEYGPVNFEYTGRKNGALVSMST
                     EKAVAFALDTLQERGHLFISPGDACYEGMIVGESAKEQDIVVNVAKTKHLGNQRASGS
                     DKAIQLTPPVTFTLEEALEYIDDTELVEVTPKHIRLRKKLLTATERKKAHQL"
     gene            20488..20940
                     /locus_tag="AC04VQ_00650"
     CDS             20488..20940
                     /locus_tag="AC04VQ_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552774.1"
                     /translation="MKAFKKFKSMTNQGEKFGGEAVKVLRKMPMWQRIVSAIVAFLLV
                     FLLWPADAAKTITAWANDAETQVATEEGVTDPDPESPDPGGGGEESDDPEVNPEPDPE
                     IEPEAEPEESAGSETSAESFDQETDENANFLDASNPVPNLTEVCGPFE"
     gene            21535..21711
                     /locus_tag="AC04VQ_00655"
     CDS             21535..21711
                     /locus_tag="AC04VQ_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552775.1"
                     /translation="MNVSDTADDTLIFQLFDIPDASSPLKFRLAREVEFDTSLEEGEG
                     EVTLNMLPATSYQH"
     gene            21754..22353
                     /locus_tag="AC04VQ_00660"
     CDS             21754..22353
                     /locus_tag="AC04VQ_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552776.1"
                     /translation="MRTDPVVINPDETTELDLSLEYPKIEADIFNKYVGTGETTIHNI
                     FATYEEDSGAIRNMNLLVDIEHGKSGNIVIDKVTSSGVLTLKAGLENASVYSIYCDVV
                     RNSKIVTDLVDAGHYEVINAGLTSTHNITLSALCYGDDESSEQPVSIVTCDVLVLVPS
                     TSGGYFQKQVHESGRGQHDVIYKVNDGDLLRVIPKPYGL"
     gene            22414..23058
                     /locus_tag="AC04VQ_00665"
     CDS             22414..23058
                     /locus_tag="AC04VQ_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552777.1"
                     /translation="MRAQIQLPSYSDFEKSADGKNYSITANYVAYNEITQQMVDKSVT
                     ISTVSKTDDGYIKRTITQRASDGVTGLEDLEANFSAVEYYSTQYKYDCEVEALSGPHV
                     CEYGTFETSLKGEYDKLPVPAKNESATYYLDGDVYVKDMATIQKGSTVSLCLNGYALI
                     MQKDSYLDVLTGDNSFNASRTKTFYNNKGTLNIYDCQKGKESCRHYFNRSSDGT"
     gene            23080..23448
                     /locus_tag="AC04VQ_00670"
     CDS             23080..23448
                     /locus_tag="AC04VQ_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552778.1"
                     /translation="MFSVDGGVITGAQKSAIYNEGTLNCEGASFIGNTNAYKSSNFGE
                     GSGGGAIRSGTTSSGYYPTLNLKSCLFEGNTSQGAGGAVLVGVSNSDCTSGTATIDNC
                     RFEFNGLIGETSSTTGYNHC"
     gene            23476..24045
                     /locus_tag="AC04VQ_00675"
     CDS             23476..24045
                     /locus_tag="AC04VQ_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552779.1"
                     /translation="MSGNDPVLYIKNNSEFYNNTSTFRGGAILLFHGTKCYMSDTTVQ
                     NNISGLYARTHSTTFNYAGGINLYTGFGNENSNPAFLYLSGKIICTENHSTTTKGERV
                     EDNLCLQVQNLATKGYINFEQNKLLDPESVIGVNYIDNHNLDISKGYGASYIPAAEKN
                     DPTVKYFILDEVAGRLSYALLIDIDSGEL"
     gene            24139..24651
                     /locus_tag="AC04VQ_00680"
     CDS             24139..24651
                     /locus_tag="AC04VQ_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552780.1"
                     /translation="MSPRPDLLKVKCSAGTTQVSGHTFSKCKYEKNECTMYLHSEDDS
                     STYILGQYIPYDGEQHGVVCDFKNEFEGIIDEKPIDYDARTNVDGIIYLDADNNILSD
                     VPSEPGIYCAAIRYYDVQISNGQKVVNTNIVHTIQSNYYEIRGPEVSINVGDISKVYD
                     GTNEARATDC"
     gene            25042..25239
                     /locus_tag="AC04VQ_00685"
     CDS             25042..25239
                     /locus_tag="AC04VQ_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552781.1"
                     /translation="MTYSRDDDEQISESGHELVPSSYAAKLQEAKVEFKLVSITGNNP
                     DVVGIDINGDGTSEYSEYYPY"
     gene            25267..25524
                     /locus_tag="AC04VQ_00690"
     CDS             25267..25524
                     /locus_tag="AC04VQ_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552782.1"
                     /translation="MKAESFKNCSVSINEPDKGEIVSDNGQKVDNVRMFYNAAGDNYS
                     AQVVNDQLSHTYALRILDQKGVATYLNYSIKEDYSSYVFSK"
     gene            26011..26550
                     /locus_tag="AC04VQ_00695"
     CDS             26011..26550
                     /locus_tag="AC04VQ_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552783.1"
                     /translation="MTYNIEPINPTIDVEVTPDPVHVVINAETTINYTVKVTNNSNVN
                     FGTVAIFSSLLGIQDLGIATYLSRGQSITLNVEDFPQLKLSSINETMISNNEIRNEVT
                     AMARISGDTSGGKLARGSGYGLTTVEKEKVQKPTARDLTYNSEEQVGVEFNEAGVDPV
                     SGFSAVEAGTYTASFKLKA"
     gene            26623..26952
                     /locus_tag="AC04VQ_00700"
     CDS             26623..26952
                     /locus_tag="AC04VQ_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552784.1"
                     /translation="MKLTPEDYTQPYNGKAYSKNFSESIDPEQYYGDDQRITDAVFTT
                     KGVNKGTYNLSQLDITYTDVPNYILSIDEESAVAISAKDFNDQTITITTSNLIYNGNE
                     QTTNYQI"
     gene            27103..27669
                     /locus_tag="AC04VQ_00705"
     CDS             27103..27669
                     /locus_tag="AC04VQ_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552785.1"
                     /translation="MTQPDGWRISKASLTVVITGNSNSVVYSGNEQSLSFDEGKGGKV
                     EFSSNPQGLTKEVVGAIAPDPYALVAKGTDFGTYSMFNDEDGQPIIFSLSEQADNINA
                     TFLTNAGILSITKKNIEDEDVAISGDTPLIYNGGVSNPNISVTYNGDALIKDVEYSIQ
                     GDSEHDVGKYTATISAKGNNFTGTKVFN"
     gene            27895..>28166
                     /locus_tag="AC04VQ_00710"
     CDS             27895..>28166
                     /locus_tag="AC04VQ_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552786.1"
                     /translation="MGNYNFDYSSLSASISKRALKVIPKSRQFEYDGNEHGLISNEIF
                     YADPDDESKEGLLTNHYVDVNKGGVTWSANNKALEVTDEPIVVNVE"
BASE COUNT         8735 a         5135 c         5805 g         8491 t
ORIGIN      
        1 ccagtttggt aattttttta tatttttaat tattttctgc atattttttt atcttctttt
       61 ttgtttcctc aactattttt tcaaattctt cttctaatgt ttttttgttt gctatcaata
      121 atgttttata ttctttattg atatttattt ttttgtaaat ttctcttaac cttcgtttac
      181 ttttatttct tcaaactgca ttaccgttct tttttccagc gataaaagct acttccccct
      241 taattttttc acttttatag aaatataaac tcaaaaatgg aaggttaatt ctttctccat
      301 ttttaaatat taaatcaatt tctttgcttg attttatagt ctccaatttt tataaagaga
      361 ctttctttct gttttttgca cgtcttgatg ctaaaacacc acgtccaccc tttgtagaca
      421 tgcgagaacg aaagccatga actttggctc ttttcctctt gtttggttga tacgttcttt
      481 tcattatatc ctcctaaaac attaggttat atctaattct cgtttttcgc gagtggtcga
      541 gtattataac aaattattta gttttttcaa ctgttttata aggtaattaa ctcatttgta
      601 atattaggag ttaaacactc ataaccatct tcggtaatta aataatcatc ttcaattcta
      661 acaccacaaa ttcctggagc ataaattcct ggttctattg tatgaatgtc attaacttga
      721 agttgatagt cagttgatat agttaatttt ggaagttcgt gattttctaa accaattcca
      781 tgacctaaac catgaactaa atcataacca atttctttaa gaacactatc tgcatgttta
      841 tgaattttat ttgtgctttc acctggtttt aacatcgaaa cacattcagt ttttgccttt
      901 aaaacttggt tatatatttc catcatttca tctgaaactt taccaattgc aatagttctt
      961 gtaatatcag aagtataatt ttgataagtt gcaccaaaat ctatagttat aaaatcacct
     1021 tgttgaactt ttctttcagt tggaacacca tgtggcgaag ctccattatc cggtcctgaa
     1081 acaacaatag ttgggaaagc tactctggaa cttcctcttc gtgacatttc actatttaaa
     1141 atattttgaa tttcaagttc tgtcatccct tctttaattt gatctaaagt ataaagaagt
     1201 gattgttcag ctatgtcaca tgcttttttt gttaatttaa tttcatcttc agtttttatt
     1261 cttctaaaat ttgttattaa ctctgtgcta tctacaatat tttcaatatt tgcatctaga
     1321 gaaagagcat ctttataaga aattgcttca aaattaataa gatatttatt tatattattt
     1381 tcttctaaaa cttcattaac tttttcaaat ttattaaata tgttttcaaa caaaattaaa
     1441 tcaaaacctt caacttgctc attaaattga ttaacaaatc ttccatctgt cataaaaatt
     1501 aacttatctt tagaaattat tgcaacagaa ttattttctt tatcataaaa accagttgta
     1561 tataaaacat ccctattttt ataaattaaa attgcatcta catttctttc ttctaaaatt
     1621 tttcttaatt taataattct attttcaaaa tcttttttca taattaacct ttctaaaaat
     1681 caaaaaaata atattaataa agttagtaaa attgttaatt tttaataaag ctccatatca
     1741 attaaataaa aacactacca aaaaaatcaa caataaaatg ttattttaaa tccctataaa
     1801 aatttttaaa ctttattaac aaaatattaa caagaaaata agggaaaaaa taacaacaaa
     1861 tccaccctaa taaattaatc cttttccaaa actctttttt ctaataattc aatttctcca
     1921 gaaatttcta tattattttt taacttttct tcaataactt taattgaatg aagaacagtt
     1981 gaatgatctc tattaaaaga ttttccaatt gcagtaacag aaatttcgag cattttataa
     2041 caaagataca ttgatatttg acgagcaaaa gcaatatttc tatctttctt ctttccaaca
     2101 agtgcgttat gacttacacc ataatattct tcgcaaactt tttgaatatt ttctacagta
     2161 actcttttct ttatattttt actaaagtgt tcttccaaaa tttcttttac tttgtcaacc
     2221 ggaatatttt catgattata tttaatttca tataaaactt ttgtaacagc actcttcaat
     2281 tcacgaatat tcgaggacga attttgtgca ataaaattaa caacatcatc tgatatgtaa
     2341 ttttcaattt ccggttcttc tttcttaaaa tcatcaatat aacttttaat aatgccaagc
     2401 tttacctcga cttcaggcga agtaatttca caagacatac cattattaaa acgcgattga
     2461 tatctttcat caacatcaat atttttagga gcacggtctg ccgaaattac aatttgcctt
     2521 ccaagatttg taatattatt aaataaagtg aagaaagtgt ttagcgttgc aaccttatct
     2581 tgtaaaagct gaagatcatc gataagtaaa acttgagcac tttcgtactt cttattaaaa
     2641 ttaacaaaac ttgatttatc aacatcatga gcggccgaag cagcagtgta ttcatttaca
     2701 aaagcagttg catcgatata aacaacgtta attgccggat tatttatatt gatgtaattt
     2761 ttaattgcgt gaagaagatg agttttccca acgccacttt taccataaat aaacagcgga
     2821 tttaaattag gctttccagg agtttctgca acagaacgag ccagttgaaa agccatatta
     2881 tttgaactac caattacaaa gttctcaaaa gttaataagt tggtcaaatt catatcgaag
     2941 tttggagttg cggttacgac ctcttcttcg gagttttgag aagtaaaaac attcgaagga
     3001 tcagtgtcaa gttttgtgtt tgaagaaacc aagtttgcag cgatatagct ttccgaagtg
     3061 tattcggtgt cggcgtcttc ggtttgatca acttcaattt gaacattaaa ctctgtcccg
     3121 taaagcgaag acagggcctg tttaatcaaa ccaagaatgt ggctttcaac ccaaaacttt
     3181 atgaaagaat tgtttgtagt tagcatcaaa aagtcgtttg aaattgcttg tggctttaat
     3241 tgcggcaaaa aagcatcaac tttttgagcg ccgccaacat caagttcttc gaccttttta
     3301 actatcgtac ctcaagtttt gtttatgtca gaaaaatcat gttccatagt tttccacaat
     3361 cgtgaaagga ccgcttacta tagcactttt gatttcaata agttcgatga atttttaaaa
     3421 ttcaaattat tgattttatg actgagagta aatatgttat catactgacg ttttttcgag
     3481 aaatggagat attatggaga aatttaggtt attggaagaa aaaaccaagg gtcgcatctg
     3541 acttattcca gcattaattt gtactatctt tgccactggc caatattgtg caccttttgt
     3601 tacttggtta taccctttgt tgttgtttag agttgcaact tatgggaaaa agaacagcgt
     3661 tttaacttat gttttgattt ttctaacaat ttatgtaggt tcagtaattc actaccaata
     3721 tgctttatta ggagaaccct gaattacaac aacgcttgta gttttagttt atcctttgtt
     3781 ctttatttga gctttggtaa tacctgcaat tttgtataga aagcttgaag aacataatat
     3841 tttaagaatt cttgtattgc cttgcatttg aattgttatt gaatatggct tttcttatct
     3901 tggatttggt agtgtatttt ctatcggatc aactcaagaa gatttttatt cactttctca
     3961 atgtgcttca atttttggtg tttttggaat cagttttatt atggtttttg catcatctat
     4021 tatttgtgat atctgaaatc acaagtttca gttttcaaaa tttaaatttt tgggtaaaga
     4081 aatggcttgt cttgtacttg taattgtttt atgttttagt tatggaagta ttaggcttgc
     4141 tcttaatcct aaaagtccgg attcaacccg tgttgcttcg gtactaaact tttatggaac
     4201 aaaagatttt ataccaacag aagaaggaat taaattatat caagacgagc atgtcaatga
     4261 atctcttgta tatatggaac aagatgttat taacgcaaaa aaagaaggtg cttctataat
     4321 tatgtgacaa gaaatttgtt tcaatgcaag ctctcagaac atagaccatt ttctaagtaa
     4381 ggcatcacag tttgctaaag aaaatgaaat cgctctttta ataccaattg acatattgca
     4441 agagcgcggt gagaaacgtt taaaccaggc tatatttata aataaggatg gaaatgttga
     4501 ttttcggtac gataaagtaa atttggttcc tattgttgag acatttgatt ataaagaggg
     4561 agagcctttg ctttgtcatt ctactgtaga tgactttaag gtttgcgcag caatttgtta
     4621 cgacgctctt aatcctcagt ttattagagc acatatgcac gaggatacag atattctttt
     4681 agtaccagct ctagaatgag aaagtattgc aaactttatc ggaacaagtt caatgtttag
     4741 agctgttgag catggtgttt cttatattcg caacactggt gctggtattg catttgcatg
     4801 cgatcagctg ggtaggcctg ttagttatta taatactctt ggagacaacc caagtatttt
     4861 atacattgat cttcctaccg atcataaagt tacaatttat caatatattg gggacacaat
     4921 tgtttattta agtattttgt attttgcgtt tattgtaatt tatgtaattg ttaaaaacaa
     4981 aaaaaagaaa tcgcaaaaaa aatcaaaata atttaaagga tgctggcaac ggcgaagcct
     5041 tgatctgaca agattcttcg cttgttgcca actctttgaa caagaccact ttgttcaagg
     5101 cttgttaaaa ttttatgagt gcttgaaagc ggaatattta gaagatcttt tacttgagtg
     5161 acgcctaagt cgccttcggt aaggaaaagc ttcaaaactt cacgctcacg agctgaaaga
     5221 gttggcgaga gcttcgtcat tttttcaaca tatttagtat cactttgttt tttatcgaag
     5281 tccgaaaatg ccgaagaact gcttaagttt tcgagcatgc ttattgtaac aacagcaccg
     5341 ttttctaaat tattttcaat ctttaagttt ccgtctgaat aatttaggta gtctttaacg
     5401 gttggaaggc cagaaccaac gcctcggatg tattgcttca ttccttcggt cgccgaagtg
     5461 aagcccggca gttgagcgtt ttctgtgtct gtaaatccgg ggccttggtc tgtgaatcga
     5521 atcgtgttgc cgttgtctag aattgaaaca acaatttctg tgaatcgtgc gtgaataaag
     5581 ttttcagaaa tttctctaat tacagtgtat ggaattttgc tgcctttttt gtttgcttcg
     5641 tcataaattg ttgatgcgag gttttcaatg aagttgtttg ttatatctgg cgagacatca
     5701 attacgcgag ggggagcctt aaggtcatcg tataccgcaa cgcgagcagg cgttcggacc
     5761 acactaaaat cttctctctg gagcgagcta taagatgtct ttctatgttt agtttctgtt
     5821 tcagccattt tcttttgttt tttatgtttt cctttacttt cattttaact tgaaagtttt
     5881 cataaaacga acatttgcga atccccactt acaagttatc tttggtgaaa gggagaacta
     5941 ttatacataa aattcattta atagcaagaa atatttttaa tgctttaagt tggcttttct
     6001 cagttatcca caatttattc acaaattgtg gaaatcttga aaaatgagaa aaaattgctt
     6061 attttgttcg gaaatttgtt gatttctttg ttaaaaatca taaaatccct gataaatttg
     6121 gcctagaatt ttggcttaaa tactgatgat taaaaaacga ataaaagaac acgaaaaacg
     6181 aacaaagcag gaataaaacg aacaaatatt cagatgaaaa acaccaggta gacagcctca
     6241 ataattcgta aagcaggaag tttagaaaac cgatatattg ttctaaaaat ttgaaacatt
     6301 ttggttttta ttatcaaaaa tggccacatt tccacagcca tatgctcgta taatgtagtt
     6361 tatccacaaa ttccacaatt ttttgaaatt tattgtggaa atcttttcct ttgttctaaa
     6421 aaaacgaaca accccgaatc ccaaaagatg taattcatgc caggtaataa ttacaccttt
     6481 tggtgcattt catgcctggc aataattaca ccttttggga aattgttgtg cattatatag
     6541 ttttatgttg taaaattcgg ctatggccac atctaagaaa aatacaaaga aaaagactga
     6601 aactaaaaag aaaaaaacta ctcctaagca ttcttcaaaa aatgttggga agaaaacaaa
     6661 caataataag gctaaagcaa aaactactaa aaaagaaaca aaaagtacta agtcaaaatc
     6721 aacaaaaaaa gataataata aaaaagaatc aaagaatatt aaaaattttg gtgtctatat
     6781 taaagataaa ttaccaaaaa agaaaccaga accaaaaact aataaagaaa agagattaca
     6841 aaagctcgaa aaattgcgtg ctgcaaaaag gagaaagaaa attgccgcaa ttgttggagc
     6901 atctttggcc ggtgtttatg ttttaggttg ccttctattt tcgtttgttt gttttccaag
     6961 aacaatagtg gctgacacag atctttcgtt tcagacaaag cacgttatgc aactttcgtt
     7021 aatcccgaat gctggagaat acgtttttaa agctgatggc ctgggcttcg aacttgaaat
     7081 tgacggaaag gcaatggact acaacttcga cattgaccag gtagttgacc gcgtaattga
     7141 gaagaaaaat ccgctcgctt ggccttatga aatttttatg gttcatgatt ttatgactga
     7201 ttcggttgta agttttaata aagacgttgc aacaagtctt gctgttcctg caattgcggc
     7261 ccacaaccaa aaagccacgc cgccagaaaa cgcaaacttg gtttgtgacc caatgacaaa
     7321 ggacattagt atcaaagacg aagtcatggg aaacactctt gacccggata agaccttggc
     7381 ttcgatactt ggatatattg gatcgggcag gcgccattta gaatgcggcg aagacgagca
     7441 agtccttcca actctttttg ccgatgatga cagatgtgaa gatgcaatcg aacaagctaa
     7501 agttttggtc gagtcgctcc ttacattaaa tatgggtggc acgaatgtgt cgactcttaa
     7561 tttgactact tgggtttctt ggttgcagct taagccaaat tatgttgctg ggcttaacga
     7621 tgaattgtta aatgcctggg ctgtgaactt ggccgaccag tgtgacacgc tcggaaaaga
     7681 gcgacatttt gtaacgcctt atggcaaaag ttgcactgta aaaggcggcg acaacatcgg
     7741 ttggcaaatc gatcaagagg cgctaaaaac tttggttgca actcaagctc ctcttggtaa
     7801 agttcaaacc ctgaccgttc cgtgctcttc gacacttaat ggttatggag gacctggtca
     7861 gcgcgattgg ggtcctcgtt atattgatgt tgatttgagc cagcaaatgg caagaatgta
     7921 tgacgatgct ggaaacatga tttggtcgag cgcgattgtt tctggtaagc ctggaaagaa
     7981 tactccgaca ggcgtttaca caattaaagc gaagagcagt ccttcgactt tgataggaaa
     8041 cattgatcct gcaacaggca agcccgaata cgagaccact gttcaatatt ggatgggctt
     8101 tgttggaaac agcattggtt ttcatgatgc cacgtggcag ccaaccttcg gtggtgattg
     8161 gtgaattaca aacggaagtc atggttgcat taatttgcca tatagtgctg ctcaaagttt
     8221 atatggttgt tgtagttctg gcgacatcgt tgttgttcat aattaatagt gaaggcttct
     8281 tcaaaatatt cagattttat tgaattctta aacgcgggta aactttgtgt gtttgaaact
     8341 gataccgttg caggcttggc ctgcacaatt ttatgtgacg gtaaaataaa taataatatc
     8401 caaagaattt ataacatcaa aaacagaaat cagagtaaag ctttgccatg attaatttca
     8461 tcaaaagaaa tgttaaatga atgggcgata aacattaatg aatactcaga agattttatt
     8521 aaagaaaaat gacctggcgc tacaacttta gtgttcgagg ttaatgatag gttgcctgag
     8581 ggtttgggag tcaacacagc gaaggaccag cacacggttg cttttcgtat tccaaattgc
     8641 gaagagctga tcaacgcaat cgattttgtt ggctatcctg ttgcttgcac ttccgccaac
     8701 ttaagcgggg atcatgcgcc aaaaactatc gaccgagttc ctaaaaaatt gcttgaacaa
     8761 gtagactttg tttacgataa aaaacaaaaa ccatctggcg aacccagcca aatcatctcc
     8821 tgtattgggg aaaactcgga aattctgagg gccgggggca gcggcgctta attatttatt
     8881 agtatttttt attttgtaac ttgcattctc gctctcgggg gccgggggca gcgagagttt
     8941 attatttttt atttttattt attttttatt taattttgtt taataattat tattatctac
     9001 cagggctttt tctgaaagtt tttggtagaa atacaaatct ttttgtatat aatcaacttc
     9061 ccaaagtttg ttaagaaagg aagagcatgg ttagaaaaat tagaaaaatt agtcaaacag
     9121 gatattatca tgtaatgtct agaggaaaca ataagcaaat aatttttaga gacgaaagcg
     9181 atcgcttaaa gtatttatct tgtatcaaag atgctttaaa gaagcacaac ctgaaattaa
     9241 tttgttattg cctgatgtca aatcacacac atttagtaat ttacgataaa gagaatcagc
     9301 tttcattgtt tatgttaact cttaatagta aatacgcttc atactttaat aaaaaatatg
     9361 agagagtggg ttatttattt caagaccgtt ttaggagtga gtgtatagga gacgaaagac
     9421 aacttttagc tgcttataga tatgttttaa acaatccgta taaagctgga ttatgtagac
     9481 cttgagaata caaatgaaat agttatgctt gcttccagaa accctcttca attgtagata
     9541 caaatattat ctctgacctt attattgacg aaaaccaaca taaagatttt atttcctcat
     9601 atgctgatga acatttcata gatgtcgaat aataaaaatt attaaacaaa attaaataaa
     9661 aaataaataa aaataaaaaa taataaactc tcgctgcccc cggcccccga gggcgagaat
     9721 gcaagtaaca aaataaaaaa taataataaa taattaagcg ccgctgcccc cggcccccgt
     9781 tgtactggcc ccggggaatt agagtgttgt tgcgccggtg tcggagaact ggatttcggt
     9841 ggcggcttcg cggccgtaaa tttcgttgac taaagtgcag aatttggaga cttgttcaat
     9901 tggcaaaaag acctgcatac agcccccaaa tccacctcca tgaattctac ttgcaccgcg
     9961 atttgtatag tcgccttcgc aagcttggtc aagacaaagc tcgcaaatag cttggcacag
    10021 agtggcttgc tggtcttctt cgctgactgt aacattttgt aaaaattgag ctgacgaaga
    10081 accagaaagc ccgtgaagtt gcataaacaa cttgccatcg ccagcctcca ttgctttgat
    10141 gcgttctttt gtaagattga tctcatggaa gtaattgatg cctcggttgg cagctttgtc
    10201 gcctaaaact ttttcagcat tcatcaaatt tttcgaaagg gtatcaatat tagtttcgtt
    10261 taaaaattca acgccacaaa gctcggcgac ttttttcatg tcgccaggaa tggcagcata
    10321 catgtgcgaa atgtcaccat gctcagctcc gctatcgacc aaaacagcac ccagaccagc
    10381 acgttcgaag ctaaagtcaa ttggcttgac gtccaggact tcggggtttg caaagttaat
    10441 cttaattatg ccgccatacg aaattgctgt ttcgtccaaa agcccgcaag gcttgccgta
    10501 atattcgacc tcggcaaaat gcccaatttg cgcaagctcc atcttgctga acttaccgct
    10561 tgcatatgcg tcgtttagga tcacggcaat taaaagttca aaagcagctg acgaagacaa
    10621 gcccatacct gcgccaatgg ttgacgagca attcgcggtg aagccagaaa ttggcacgcc
    10681 agcttcgatg aatttatgcg caacgccggc gaccagagct tcggtggtat taaacttcga
    10741 agggtcgggc gccatggctt cggcaacttt gtcaatgtca acatcaaatg gctcgaaacc
    10801 ctcactatta atatgtatga ttttgtcatc acgaagctca acatcggccg aaatcccctg
    10861 cttgaggctt cctgtaacag caattccatg ctgatgatca acgtggttgc ctgcaatttc
    10921 agtgcggccc ggcgcgaaag ctttaaagtt cttacccatg attttccttt cagataccat
    10981 ctatattata ttgtaaccta tattttacaa tagaaaaaat taaaaaatat taaataagaa
    11041 ttaacaaaaa taaaaaataa ttaaatctca gtgcccccgg ccccgatggt tataatataa
    11101 tatagtgcgt tatgggaatt tttaacagaa aaagcgaggg cggacgcaaa aaaccaacca
    11161 ctggattcag tgaacttaac cgccgaactt acgaagccag cccaaccggc cgcaccggac
    11221 aaattgaccc aacaaacact gttggatacg ctggcaatcc aaatgctaaa aaacagagcc
    11281 gaagacgagg cgccgacctg ggctttaagc ctcaggagtc cctcagcgat tattcgcgtc
    11341 gtggacagca acaaaaatac gctgcggcgc gcaaggctca ggataagaaa aagcgcaaaa
    11401 aaaagatcat aataattgtg tgttcgattc ttggtgcact ggttgctgcg ggcgcgattg
    11461 ccttcggcat tataagttgt aatttaaacg ccggaatggg caacattgga ctcactccga
    11521 ccagcttgga caagcctttt tacatggtgc tcatgggtgt tgattcttcg gacgttcgca
    11581 aagccgaaag tggcggcgat gattcggact accgttccga cagcatgatt cttgctcgcg
    11641 ttgctgctaa cgagaaaaaa gttacgctca tgtcaattca ccgcgacatt gaaatggaca
    11701 tgggtggtga atatggcgtt caaaaaatta atgcttcgca ttcgattggt gggccggagc
    11761 ttgtcatcaa aaatgtgtca aaacttgccg gcgttccaat taaccattac gccgaaatta
    11821 acttcgacgg tttcacggcc gcggtaaatg accttggcgg cgttgatgta aatgtgccaa
    11881 tagagattaa tgaccccgaa gccggcggtt acgttccagc tggtaatcag catcttaacg
    11941 gccaagaagc tttgatttta gccaggtcgc gccacgctta tgatgcctac ggtgacggcg
    12001 ataaattccg cgcggcaaac cagcgcatgc ttctttcggc aattgcgcaa aaggctcttg
    12061 attctgacat catgacaatt gcgcaaaccg ttatggatat gtctggctac atcaaaactg
    12121 accttgccat caacgacatg attggtcttg ctcagttgat gaagggtcta aaccctgcga
    12181 aggatgttta caccgcaatg actccaactc aaggcgttta taaaaatggt ggttgatacg
    12241 aagagctcga cgaagttgag tggaaagcga tgatgaagcg catggatgct ggtcttccac
    12301 ctaacgaaga gccatacatt gaccctgcta caggcacggt tatgagcact tcgggctcgg
    12361 gtgactggga aggaacggtc agcgttaaaa atggatcggg agttgatgga gctgcggctt
    12421 cggcttcgtt gaaaattatc gcgcttggat tcacggccac aacgggcaac gcggacaaag
    12481 acaattattc gaaaacacta attatatata aggacgatga caaggaagat gccgccgaag
    12541 ctatcgcgga caaactcggt tgcgggcgtg tccagcaaaa tgacggcgac tatgattttg
    12601 acactgacat tttggttatc atcggcctcg atttcgaata aatttcgaca aaaatttaga
    12661 cttgctatgt ggcgtttgtg gtaaaattgc cctgctatta aattcgttta aaagagagga
    12721 ttattatgga ttacgctaaa gaatctttaa agaaacatgc tgaatggaaa ggtaaaatca
    12781 gcactgagct aaagtgtccg ctggagaatg cgactgatct ttcgctggct tacacgcctg
    12841 gtgttgctga gccttgcctt gctatcaagg atgatattga gcaaagttat aatttgacca
    12901 tgcgcggaaa cctgtgcgcg gttattactg acgggagcgc ggtgctcggc ctcggaaata
    12961 ttgggccgga agctggcatg cctgttatgg agggcaaatg cgcgcttttc aagaagtttg
    13021 ccggcgttga cgcaattcca atttgcgtta aaagtcagga tcctgacgaa gttgctcacg
    13081 ttgtgcacat gatttctgga agttttggcg gcgtaaatct tgaggatatt tcggcgcctc
    13141 gctgttttga gattgagcgc aagtgcaagg aactttgcga cattccaatt ttccatgatg
    13201 accaacacgg cactgcaatt gttgttctag ctggtcttat taacgcgctt aaagttgttg
    13261 ataagaaggc cgaggattgc aaagtcgtca cttgtggcgc tggtgctgcc gcgactgcta
    13321 tcgttaagtt gcttcttagc tatggtttta agaacgttac gatgtgtgac agcaagggcg
    13381 tcatccataa ggatcgcgat gacctcaatt ggatcaagaa ggaaatggcc gaggttacaa
    13441 accccgaagg aattacgggc agcctcgccg atgcgatggt tggtgcggac attttcatcg
    13501 gaagtagcgc gccaaacact gttagcaaag atatggttgc aagcatggct gagaaatcga
    13561 ttgtttatgc ttgcgcgaat ccaacgcctg aaattttccc tgatgatgcc aaagaagctg
    13621 gtgcgtttgt tatggcaacc ggccgttctg atttcccgaa ccaggttaac aacgttttgg
    13681 cattcccagg aattttccga ggcaccttcg atgttcacgc aagtgacatc aacgaagaaa
    13741 tgaaaatggc agctgcaaaa gcaatcgctg gcatggtcag cgaagatgag cttggtccgg
    13801 aatgcattat tccttcgcct ttcgcagaag gtgttggcga agttgtagcc aaggctgttg
    13861 ccgaagctgc aattgcaagc ggagttgcaa agaaaaaata aggaagtgat ttaaatggca
    13921 gcaacgataa gtaggggcgt tcgcgcgcca ataattcgcg aaggcgacga tattgttcaa
    13981 atcacggtgg acagcgtcct cgccgcatgc aaagaagatg gctttgagct gggcgataag
    14041 gacgtagttt gcgtcaccga aagtgttgtt gcccgcgctg ccaaaaatta ttgctccacc
    14101 gatgacattg ccgcggacgt caaggcaaag cttggcggtg agaccgtcgg catcgtcttc
    14161 ccaattttta gccgcaaccg ttttgcaatt tgcctgcgag gcattgcccg cggtgcaaaa
    14221 aaagttgtgg tccagatggg ctacccaagc gacgaagttg gcaaccacct cgtcaccgaa
    14281 gaccaaattg atgacagcgg cgtaaacccc tacagcgatg tcctgaccgc cgatgaattc
    14341 cacgctaagt tcggcgacat aaaacacgag ttcacaggcg tcgattacct cgattattat
    14401 aaggagctca tcgaagacga aggtgccgaa gccgaggtca tcctcgcgaa tcgacctgag
    14461 gcaattttga aattcaccaa aaatgttttg gtttgtgata ctcacagtcg tgatagaaac
    14521 gctcgccgaa ttaagaaggc tggcggcgaa acagttctaa caatggctga cattatgaat
    14581 gcctctgtta atggtagcgg ttacaattcg aagtacggtt tgctcggctc gaacaaagcg
    14641 accgaaagca cggttaagct ttttccggaa aatgcgcaag aagttgccga agcaatttcg
    14701 aacaaattat cggaaattac tggcaagcaa attgaggcca tggtctatgg cgatggcgct
    14761 ttcaaggacc ctgtcggtaa aatttgggag cttgctgacc ctgttgtttc cccaggttac
    14821 accaaaggtt tgaccggtct tccaaacgag cttaagctaa aatatttggc ggacaatgac
    14881 ttcgccgatt tgagtggcga agaactccaa gccgctgtga aaaaagcaat cagcgaaaaa
    14941 gaaggcgacc ttaagggcaa catggcatcg cagggcacga cgcctcgcca gattactgac
    15001 ctcattggtt cgctttgcga ccttacaagt ggaagcggcg ataagggcac gccaatcatc
    15061 gttgttcaaa actacttcaa aaactactcc atgtaataac gctgattttg tagctcaaat
    15121 ttcaaatttt gtagctcaat aattgaaata atggctcaaa tgcatctcgt gtactaattt
    15181 tgtagctttt cagcgccatc tttatttctt ttgaagaaaa acacctgtac aaatttttta
    15241 taaatacata tttgtgtaaa aaaacatgaa aaaaccgtac acagtttgag tttgagccaa
    15301 aaaatcataa attgagctac aaaaataact taaataacta aatttgttcc gatagcgcca
    15361 ttcatagttt tcttcttttt gtgtcgaaag tttttgtacg tattctggcc tcttgcttat
    15421 ttgcttttag tatttttacc gccatgttgt gaaaagaatg aacgtctcgt aattggctgg
    15481 aaacgaaagt gaacactttt cagccgtcat gctgaagagc gtctgctcgc aatttgtctt
    15541 ttgtgttttg gccagtgttg tcatggaatt cgttgctgtc atattctata gctatctttg
    15601 tctttttgtt acaaagatct ggtttaatgg ttgggtgttt caaaatttcc ctggcttgtt
    15661 cagataattt aatttggcaa ttggccttta tgtttgatat ttcataaccg cccatattac
    15721 gaggagcaca cagtttaata tataggttgc tttcttgagg agacgcgcaa ttgtctatta
    15781 aaagcgaaga agcttgaagc gcctttctta tgccagggag atttttgtgc cttttattta
    15841 ggttggtaat atatgtttta atttttaagg ctgttgttaa aggaggaatg ttatatacgt
    15901 atccattagc agcagattca gatatgctat aagtaccaca catttccaac cctgtctgaa
    15961 gtagctgctc gaagggcata atggtcgcca actggtaaaa caaaagagga ggtgtaggga
    16021 tataaatgtt gtcattgtat tttagaaatg agttccttaa gtatgtaatg tttgaataat
    16081 gaaattttat gttttttaaa tggcggttgt aatttttgtt ctttgtaaga agatggtaag
    16141 gtgcgtttcc gcagtagggt aagtcttcta taataacgga atttttagtt gtgcgaagat
    16201 cgctcgaagg ctgttttgaa catgggagta gcgttagcgt atttttggtc ggatcagaat
    16261 tttcgaggtc gaggtccagg tttgtgcgag catttttata aaattcaaga gccgaagtaa
    16321 tgtagataaa gaatttctgc atttgtgctt cctttcaaca acgttttggg atgttgatta
    16381 tactcaaaat ttttaaaagt ggtagaataa aattatggaa gacaggattt acattgcgat
    16441 tgatttgaag agcttttatg cttcggtcga atgcgtgcag cgaaatattg accctcttga
    16501 ttcatacctg gtagttgcgg ataaatctca cactgaaaag acaatttgct tggcggttac
    16561 gccggcactg aaggattatg gaatttcggg tcgcgcgcgg cttttcgaag ttatcgaagc
    16621 ggtggacgag attaaccgaa ttcgagggcg acggctactt gagctttatg acgatgtcga
    16681 tgccacaggt cggcatggcg cggtttcgaa aaagttcacg aagaaatgtg ccttcgatgc
    16741 ggagctgaaa agccatccgg aatatgaact cgactatatt gttgcgcctc cgcaaatggc
    16801 gaaatacatc caggtcagca cacaaattta tgaaatctat ttgcgctatg ttgcgccgga
    16861 agatattcac gtttacagca tcgacgaagt ttttatcgac gccacgccct atttgatgat
    16921 gcacgattgc accgccgaag aacttgcgcg acgcatgatt gccgatgttt tggccgagac
    16981 cggaatcacc gcgaccgttg gcattggcac gaacatgtat ttgtgcaaaa ttgcgatgga
    17041 tgtttttgca aagcggatgg agccagataa aaatggcgcg cgcattgcgt ttttggatga
    17101 gcagggctac cggcagcata tgtgaaacca tcgtccgctt tcggactttt gaatgattgg
    17161 agctgggtat actcgcaggt tggaggctta cggaatgcac acgatgggtg acatcgcccg
    17221 ttgttcgatt gaaaacgaag acctgcttta cgagatcttc ggcattaacg cggagctttt
    17281 gatcgaccac gcttgaggtt gggagccgtg caccattgca gatgttaagg cttacaaacc
    17341 tgtcactaat agtgtaagca gcggtcaggt tttgcatcgg ccatataaaa atgacgaagg
    17401 ccggctggtg cttcgtgaga tggcggatgc tctttcgcag gaactggttt cgaaaaattt
    17461 ggtgaccaaa aagcttgtga ttcatgttgg ctacgacatc aaaaatatta tggacattga
    17521 aaagggcatt gtgaacgcct acgctgggga aataagcact gaccattatg ggcgcaaggt
    17581 gcccaagtac gctcgcaaga caatcaactt gccgcgctga acttcgtcgg ccactattat
    17641 tatggatgcg gttgccgatg cgttcgacca agtggttgac aagcagctgc tgattcgccg
    17701 attcaatgtc acggctgagg ctgttcttcc aaagactcaa gttgacgaag cggccgcagc
    17761 cgcgcctcca aaagttactc aaatgaacat ttttactaat tatgagctgg aggaaaaact
    17821 caaggctgcc gaagatgaaa agctcgaacg cgagcttgaa cttcaacgcg tgatgcttgc
    17881 ggtcaaagaa aagtttggca aaaacgctat tctacgaggt atgaacctgc aagaaggcgc
    17941 caccatgaaa gaacgcaacg cgcaaattgg cggccacaaa gcctaacatt tccccggggc
    18001 ccacactcga tttactcgtg gcatttactc gatttccccg gggccagaac tcgatttggg
    18061 ggggccgggg gcagcgggag tttattattt attattattt tttatttttg gaagctttat
    18121 cgatggattc ttcgaagccg gttagcgcgg cgaagggagc aaattgcgca gcccgattat
    18181 aacgtggcat gcgagggtgc ttctcttcat caggctcatg ccgtggcata tcaataatat
    18241 ctttgtattt ttctgaagaa ttttccatgc ttctataata gcacgaaaat aaaaaataat
    18301 aaaccgcgaa taaactcccg ctgccccggc tctaaaaatc gagtactgac cccggggaaa
    18361 tcgagtaaat gccacgagta aatcgagtgt gggccccggg gaaatggtgt ataatgtgcg
    18421 ggctatgagt gtaaaaaggg aagatatccg aaatgtggcg ataattgcgc acgttgacca
    18481 tgggaaaacg actctggttg attgcttgct aaaagcgacc gatgtgtttc gccagaatca
    18541 gaaagttgac gaacgcgtcc ttgacagcaa tgatcaggag cgcgagcgcg gcatcacaat
    18601 tctttcgaaa aatgtcgcga taaattacaa aaacaaaaaa atcaacatca ttgacacgcc
    18661 tggccacgcc gattttggtg gcgaagtcga gcgcgtgctt aacatggccg atgcggcgct
    18721 gctcatcgtt gatgccttcg aaggtcctat gccccagact cgatttgtgc tcaagcacgc
    18781 gttgtcccag ggtatgcgca taattttggt cgttaacaag attgaccgcg atggcgcgcg
    18841 cccggatgcg gttgtcgacg aggtcttcga tttgatggtc gaccttaacg cgaccgacgc
    18901 gcagctggac ttcccaatca tttacgcaag tgcggcgcaa ggttacgctc gcgcggaact
    18961 gaccgacaca aacacagaca tgttcccgct tttggatatg ataatcaacg aagttccggc
    19021 ccccgaagtt gaggccgaag agccctgcgc tcttcaaatc tcaacggttg atcactccag
    19081 ctatcaaggc agaattggag ttgggcgcct cggcttcgga acgctgaaaa agggccagca
    19141 ggtcctggcc gtgaacaatc acggtaaaaa gtacaagacg caaatcaaac agttgttcgt
    19201 ttttgagaat cttggcaaga acgaagtgac tgaggccgcc gcgggtgaca ttgttgcggt
    19261 tgttggtgtg gacgatgccg atattggcga cgtgattgtg gacgtgcctg gcgatgatgc
    19321 cgctgcgatc gacgaagctg ccgcgcgcgc cgcgaacctt tttgcgccaa tcgaagttga
    19381 agagccaacc atggctgtaa tttttgagcc ctcgaccagc cctcttgttg gtcaggacgg
    19441 agagatcgtt ggcgggcgtc aactcaagga gcgtctgctt cgtgagaaag aaagcaacat
    19501 tagtatgcga atcaccgaag tcgcggacag cactgcgatg gaagttgccg gccgcggcgt
    19561 tttgcacttg agcgttttga tggagaccat gcgtcgcgaa ggttacgaat ttcaggtcgg
    19621 gcgtccgcgc gttttggtta agaccgacga agccggccgc acgcttgaac cgattgaaga
    19681 agctgtggtc gacgtggata acgaatacag tggaaaggtc atcgaggtct tcggtaactg
    19741 cggtggcgag atgaccgaga tgaacgccaa ggccgatcag acccacctcg tttttaagat
    19801 tccgagccga ggcaccatgg ggcttgccac caagcttctc aacgtttccc atggtagcgc
    19861 cacacttttc catcacttcg cagaatacgg tcctgtcaat tttgaataca ccggccgcaa
    19921 aaatggcgca cttgtgtcca tgagcaccga aaaagccgtc gcatttgcgc tcgacacttt
    19981 gcaagagcgc gggcatctct tcattagccc aggtgacgcg tgttatgaag gcatgattgt
    20041 tggcgaaagc gcgaaggaac aagacattgt ggttaacgtg gccaagacca agcatcttgg
    20101 aaaccagcgc gcttctggta gcgataaggc aattcagctt acgccgcctg tcacatttac
    20161 cctggaagag gccctcgaat acatcgacga cacagaactt gtcgaagtaa cgccgaagca
    20221 catccgcctt cgtaaaaaac tccttacagc aaccgaacgc aaaaaagccc accaactcta
    20281 aaacccaact ataggggccg ggggcagcgg gagtttattc ttttttattt aaaattatta
    20341 tcgtgatatt tattattatt ttgcgttaat tgcgcctgcg tgcgcttaat ttttgtgcta
    20401 taatgtcacg tggctataaa cgcactcaag ttgcttatgt tctaaagaac tccatagtca
    20461 aaatctctat gtttaggagg gtttataatg aaagcgttta aaaagtttaa gtcaatgaca
    20521 aatcaaggcg aaaaattcgg aggtgaagcc gtaaaggttt tgcgcaaaat gccgatgtgg
    20581 caacgaattg tatcagcaat cgttgctttt ttattggtct ttttgctatg gccggccgat
    20641 gccgcgaaaa ccattactgc ctgggctaac gatgccgaaa ctcaggttgc aaccgaagaa
    20701 ggcgtaacgg atcccgatcc agaatcgcca gatccaggtg gcggtggcga agaatccgac
    20761 gaccccgaag tcaaccccga accagatcca gaaatagaac ctgaagcaga acctgaagaa
    20821 tctgctggtt cagaaacatc agctgaatct tttgatcaag aaacggatga aaatgctaat
    20881 ttccttgatg cgagcaatcc agttcctaac ttaactgagg tttgtggccc ttttgaatga
    20941 gatttttctg catatgtaaa caataataga agcttgactg ctgtaacaac tactcccctc
    21001 caaacacttg attatgcaga caatagtaaa attgctgacc ttcaatttgt aggaccttga
    21061 aaaagcaata atgatgttta tgatggtggc gcatcagcca tcacagaaaa cggatttgtt
    21121 tctaattatt atcaccgact aaaaggaatg tcatgaaaaa ctgcagatgg tgaaattgtt
    21181 agtaactacc ttgtctttca gccaaaagcc aacatagaat ctctcgaaat ttcatgtcaa
    21241 gctgttgatg tttatacaaa atatggttat aaagaatatc ctattttagg atttctggaa
    21301 gtagatcctt cagcaacaca attcttttct gccaatgcag ttactacaaa caatattaca
    21361 agcagtgaga cttgaacaaa agtaaaatga gacaacaatg gaaaaggatt ttcctatgat
    21421 aaaaaataca tcattttctt taaacctaac gtagactatg attctgatta tgccgattat
    21481 ggcggtatta aaattaatca tataaaagca gaagcccaaa caggatcttt aaaggtgaat
    21541 gttagtgata cagcagacga tactctaatc tttcaactct ttgatattcc agatgcgagt
    21601 tctccactta aattcagatt agctagagaa gtagaatttg atacaagttt ggaagaagga
    21661 gaaggagagg taactttaaa catgcttccc gctacttctt atcagcactg aaaactaata
    21721 tccaaaagaa caggagtgta taaagagcat agagtgagaa cagatccggt tgttattaat
    21781 ccggatgaaa caacggaatt agatttatca cttgaatacc caaaaataga agcggatatt
    21841 tttaacaaat atgtgggaac tggagagaca accattcata atatttttgc aacttatgaa
    21901 gaagacagtg gtgctatacg aaatatgaac ttattagtag atattgaaca tggtaaatct
    21961 ggcaatattg ttatagacaa agttacatcc tcaggagttt taactttgaa agctggactt
    22021 gaaaatgctt ctgtatatag catttattgt gatgttgtta ggaattcaaa aatagttacg
    22081 gatctcgtgg acgctggtca ttatgaagtt attaatgcag gtttaacttc tacacacaat
    22141 ataactcttt ctgcgctttg ttatggagac gatgaaagtt cagagcagcc ggtttctatt
    22201 gtaacatgtg atgttcttgt tcttgttcca tcaacaagcg gaggatattt tcaaaagcag
    22261 gttcatgaaa gtggaagagg tcagcatgat gttatatata aagttaatga tggagatttg
    22321 ttgcgagtta ttccgaagcc gtatggatta tgaattaaga ataaagatgg tggacagcca
    22381 attaatttaa ataaatacga agtacacagc gatttgagag cgcaaattca acttccaagc
    22441 tattctgatt ttgaaaaatc agccgacgga aagaattaca gcataacagc taattatgtt
    22501 gcgtataatg aaattacaca gcaaatggtc gataagtctg taactataag cactgtttct
    22561 aaaactgatg atggttatat taaaagaact attacacaac gagcaagtga cggcgtgact
    22621 ggtcttgaag atctcgaagc aaatttttct gcagtagaat attactctac tcagtataaa
    22681 tatgattgcg aagttgaagc attatctggc ccgcatgttt gtgaatatgg aacctttgaa
    22741 acaagcttaa aaggtgaata cgacaagctt cctgtgcctg caaaaaatga atctgcaaca
    22801 tattatctag atggtgatgt atacgttaaa gacatggcaa ccattcaaaa aggaagtacc
    22861 gttagtttgt gtttaaatgg ttatgctctt attatgcaaa aagacagtta tcttgatgta
    22921 cttacaggag ataattcttt taatgcttca aggacaaaaa cattctataa caataaggga
    22981 acattaaata tttatgattg tcaaaaggga aaggaatcat gtaggcacta ttttaacaga
    23041 agttctgatg gaacttgagt gctttcttct gaatgaggaa tgtttagtgt tgacggtggt
    23101 gttataactg gtgcccaaaa atcagcgata tataatgaag gaacactaaa ctgtgaagga
    23161 gctagtttca taggcaacac taatgcctat aaatcctcta attttggtga aggttctggt
    23221 ggaggtgcaa tcagaagcgg tacaacaagt tctggctatt atccaacact taatttaaaa
    23281 tcttgccttt tcgaaggaaa tacatcacag ggtgctggtg gagctgtgtt ggttggcgta
    23341 agtaattcag attgcacatc aggaacagca acaattgata attgtcgttt tgaatttaac
    23401 ggcttaattg gcgaaacgtc ttcaactact ggatataatc attgctgagg cggtggagct
    23461 ttagctacat tttcaatgtc tggcaatgac ccagtcttat acataaaaaa taatagtgag
    23521 ttttataaca atacttctac gtttagaggc ggggcaattt tattgttcca tggaactaaa
    23581 tgttatatga gcgacacaac agttcaaaac aatattagtg gactttatgc tagaacccat
    23641 tcaactacat ttaattatgc gggcggaata aatctttata caggttttgg caacgaaaac
    23701 tctaacccag catttttata tttatcaggt aaaattattt gcacagaaaa ccattccaca
    23761 acaacaaaag gtgaacgtgt agaagataat ttatgtcttc aagttcaaaa ccttgccaca
    23821 aaagggtaca ttaattttga acaaaataaa cttcttgacc ctgaaagtgt tattggagtt
    23881 aactatattg ataatcataa tttagatata agcaaaggtt atggtgcttc gtatattcct
    23941 gctgccgaaa agaatgaccc aacagttaaa tattttattc ttgatgaagt agctggaagg
    24001 ctttcctatg ctttgttgat tgatattgac tctggtgagc tttgaattgt taagcacaca
    24061 cataaactta cttatactaa tgatacatcc ataaaacttt ttgataattt agataataat
    24121 tctggttatg gtattcagat gagccctcga ccagatcttt tgaaagtaaa gtgttccgca
    24181 ggcactactc aagtaagtgg acatacattt tctaaatgca aatatgaaaa aaatgagtgt
    24241 accatgtatt tgcacagtga agatgacagc agtacatata ttttaggaca atatattcct
    24301 tatgatggtg agcagcacgg agttgtttgt gattttaaaa atgaatttga aggcattatt
    24361 gacgaaaaac caatcgatta tgatgcgcgt actaacgtag atggtattat ttatttagat
    24421 gccgataaca atattttatc cgatgttcca tctgaaccag gcatttattg tgccgctatt
    24481 agatattatg atgttcaaat atcaaatggc caaaaagttg ttaacacaaa tatagttcac
    24541 acaattcaga gtaattatta tgaaattcgc ggacctgaag tttcaattaa tgtaggtgat
    24601 atttctaaag tttatgatgg tacaaacgaa gctagagcta ctgattgttg aattgccgat
    24661 aatggcaaag tcattattgg agacgatgta aaagttagca ttaaggaaga ttcagatgca
    24721 atagtaggtt gcgactctaa atatgcaggt aagcatgcaa ttacttcatt caatagagat
    24781 gattttgaac ttattggaaa tgatgctcat aagtatagca ttggtaatgt cattgttggc
    24841 acaataaata tcacgccagc aaatctaaat attgcatgcg gagagtttac tgcattccaa
    24901 ggcgaccctt cagttaatct taatacttta ttaagtttac ctgaagaaac atgggcttat
    24961 gcaaaaccga cttttagtgt aagagaccaa tcaactactt atgaagacgt tggaactctt
    25021 acttatgaag aaaataaagg tatgacttat tccagagatg atgatgaaca aattagtgaa
    25081 agtgggcatg aattagttcc gtcatcttat gcagctaaac tacaggaagc aaaagtagaa
    25141 tttaaattag taagtattac tggaaataat ccagatgttg ttggaattga tattaatggt
    25201 gatggaactt cagagtattc tgaatattat ccatattgag atgagtgaat tactggacat
    25261 cttgttttga aagcagaatc atttaagaat tgttctgttt ctattaatga accagataaa
    25321 ggagagattg tttctgataa tggtcaaaag gttgataacg ttagaatgtt ttacaacgca
    25381 gcaggtgata attattctgc acaagtagtt aatgatcagt taagtcatac ttatgcacta
    25441 cgaattctcg atcaaaaagg tgtggctacc tatttaaatt attcaattaa agaagattat
    25501 tcgtcctatg ttttctcaaa atgaacaatt actataaatg gtgaagaagt tccattcgta
    25561 atgatggaag gacaggagtt ttctcaaaca gtaaatttta aagcggaaga agtatcgttt
    25621 aaatgttata tcgtggaaaa tggcgtacct tcgcttgagt gagaagttac ttcgtcatat
    25681 gatcctaata ctaagctaga gcatgttggt caagaaataa aagtatgggt aaatattaaa
    25741 aatacaggta attgtccagt tgtatttcaa tatgcttgat ttaagagttc aggtgtacct
    25801 caatatcaag aacttgaaga tattccagta ggtaaaatca tttatcctga agatacattt
    25861 tatgaggaac ctcacttata tacgtttgct gtttctgatc aagacatgtt aaatggcata
    25921 ggcgaaataa taggagaagc taatgtaaaa agatctccag atgaaccaag cccctattat
    25981 tgatccgact atacagagcc atgagaaggg atgacttata atattgaacc tataaatcca
    26041 actattgatg tcgaggttac accagatcct gttcatgtag ttattaatgc tgaaacaaca
    26101 attaattata cggttaaagt cactaataat agtaacgtta attttggaac tgttgcaatt
    26161 ttttcgtctc ttcttggaat tcaagacctt ggaattgcta cctatttgag cagagggcaa
    26221 tctataacat taaatgttga ggattttcca caattaaaac tttcaagtat taatgaaaca
    26281 atgatttcaa ataatgaaat taggaatgaa gttactgcaa tggctcggat ttcgggagat
    26341 acttcaggag gtaaacttgc gagaggtagt ggctatggtt taactacagt agaaaaagaa
    26401 aaggttcaaa aacctactgc acgtgattta acttataaca gtgaggaaca agtaggtgtc
    26461 gaatttaacg aagcaggtgt tgacccagtt tctggttttt ctgctgttga agcaggtacc
    26521 tatacggctt cgtttaaatt gaaagcatga tatgaatggg aagatgatcc aacttcctcg
    26581 tattgcaccg taacctatac aattaaaccc gctactttaa acgtgaagtt aacaccagag
    26641 gattatactc aaccatataa tggaaaagca tacagtaaga atttttctga gtctattgat
    26701 ccagaacagt attatggtga tgatcaacga attactgatg cagtatttac aactaaggga
    26761 gttaataaag gaacatacaa tctttcgcag ttagatatta cttatacaga tgttccaaat
    26821 tatattcttt caattgatga ggagtctgca gttgcaattt cagcaaaaga ctttaacgat
    26881 caaactatta caattacaac ttcaaatctt atttacaatg gaaatgaaca aacaacaaat
    26941 tatcaaattt gagataaaac tcttggaaac aatcctaccc ttttagtaga aggaacagat
    27001 tatgaagtta ttggaggaat ttctgcaaca aatgctaacg aagaacctaa agctgcttat
    27061 tttgtaaaag taaagggtat tggtaattat gttgattcag aattgactca accagatgga
    27121 tggagaattt ctaaagcaag tcttacagtt gtcataactg gaaattccaa ttctgttgtt
    27181 tattcaggta acgaacaaag cttaagcttt gatgaaggca aaggcggaaa agttgaattt
    27241 tcttctaacc cgcaaggatt aactaaagaa gttgttggcg caattgcacc tgatccttat
    27301 gctttagttg caaaaggaac tgactttggt acatacagca tgtttaatga cgaagatgga
    27361 caacctatca tttttagtct gagtgaacaa gctgataata ttaatgcgac tttcctaact
    27421 aatgctggca ttttaagtat tacgaagaaa aacattgaag atgaagatgt tgcaatttct
    27481 ggagatacac ctttaattta caatggaggt gtttctaatc caaatataag tgttacttat
    27541 aatggagacg cgctaattaa ggatgttgaa tattctattc agggagacag cgagcatgat
    27601 gtaggaaaat atacagccac aatatctgca aaaggaaata attttacagg aaccaaagta
    27661 tttaactgag aaattgttaa gaaagattat gaaattacac atgaaccatt taacaaaaca
    27721 tatgacgcaa cattcttaac ttctgaattt actaataagc cttttgaaga ttctgatgat
    27781 gcaattaaag ttactgtaaa aacgagcagc gatgaagttg gagtttatcc taatttaata
    27841 agccccgacc atcaattaaa tgtaaatatt gtccctgcaa acgaaacaac caatttggga
    27901 aattataact tcgattactc aagtctttct gcatcaattt caaagagagc attaaaagtt
    27961 attcctaagt cgcgtcagtt tgaatatgat ggaaatgaac atggccttat tagtaatgaa
    28021 atcttttatg ctgatcctga tgatgaatct aaagagggtc ttttgacaaa tcattatgtt
    28081 gatgtaaaca aaggtggagt tacatggagc gcaaacaata aggctttgga agtaactgat
    28141 gaacctattg ttgttaatgt tgagaa
//
LOCUS       DBNCDC010000004        25264 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_437_length_25264_cov_7.650720, whole genome shotgun sequence.
ACCESSION   DBNCDC010000004 DBNCDC010000000
VERSION     DBNCDC010000004.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 25264)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 25264)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25264
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_437_length_25264_cov_7.650720"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(54..167)
                     /gene="rpmJ"
                     /locus_tag="AC04VQ_00715"
     CDS             complement(54..167)
                     /gene="rpmJ"
                     /locus_tag="AC04VQ_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006363204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIO9552787.1"
                     /translation="MKVRPSVKKMCDKCKIIRRNGKVLVICENPRHKQRQG"
     gene            complement(186..404)
                     /gene="infA"
                     /locus_tag="AC04VQ_00720"
     CDS             complement(186..404)
                     /gene="infA"
                     /locus_tag="AC04VQ_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIO9552788.1"
                     /translation="MTKQDVIELEGEVLEALPNAMFKVKLENGHEILAHISGKMRMHY
                     IKILPGDKVTVELSVYDLNRGRITYRYK"
     gene            complement(431..1024)
                     /gene="rpsI"
                     /locus_tag="AC04VQ_00725"
     CDS             complement(431..1024)
                     /gene="rpsI"
                     /locus_tag="AC04VQ_00725"
                     /inference="COORDINATES: protein motif:HMM:NF001099.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIO9552789.1"
                     /translation="MPTKKTETKTEEKKTTKAAAKKTATAKKATTKKAAEKKTTTASA
                     KKETAKKDTASKATASKAKAAKAKKPEALYYGTGRRKNAVARVRLYAGKGDISVNGRD
                     VKEYFNRQVLINDILSPLNVTETQTHFDIVANIKGGGTTGQAGALRLGISRALLQAGD
                     YRETLKKAGFLTRDSRMVERKKYGLKKARKKSQFSKR"
     gene            complement(1030..1464)
                     /gene="rplM"
                     /locus_tag="AC04VQ_00730"
     CDS             complement(1030..1464)
                     /gene="rplM"
                     /locus_tag="AC04VQ_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIO9552790.1"
                     /translation="MKTYYAKKEDVKREWYLIDASDQVLGRLATFTANILRGKNKPEY
                     TPHVDTGDNVVIINASKVKLTGDKAMKKSYIHHSGHPGGLKEETYEQAMEKHPERVIE
                     HAVKGMLPKNTLGRQMGMKLHVYAGEAHNHEAQKPQQIKVEG"
     gene            1669..2325
                     /locus_tag="AC04VQ_00735"
     CDS             1669..2325
                     /locus_tag="AC04VQ_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552791.1"
                     /translation="MSDSKKSENIKEKAKETTAKAKEQLDSFIEKKPIDNIKLPLPVK
                     IFAVLCIVQCVITAPLTYNYIVDFIQSIQDESILSQTASSIVMTVLFLSCTVITIALL
                     IAIGVNMLLNRRRIAAILSNIAAVTIIIASFTEVMVKGLTPEIYVQVITIVLLIALKS
                     YLDPSLSKERKQHRHERDQQNKKEQESGNLGFHSDGRPKLNFFNLFWLFCIMCVVGYL
                     "
     gene            2332..2562
                     /locus_tag="AC04VQ_00740"
     CDS             2332..2562
                     /locus_tag="AC04VQ_00740"
                     /inference="COORDINATES: protein motif:HMM:NF018271.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIO9552792.1"
                     /translation="MWHMTVDNPGVYEERAGFLFGPFSPIYGFGACIITVILNKFYNK
                     NFIIVFIVSALLGGFFEFFVSLYLETCFGIRS"
     gene            complement(2575..2973)
                     /locus_tag="AC04VQ_00745"
     CDS             complement(2575..2973)
                     /locus_tag="AC04VQ_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552793.1"
                     /translation="MAYREAVEKCTFKISLVVIIATADGGVWIHLHVFEALMHEVIIE
                     VLLVELFKRRLWRTASVSSVSAVESVECHNHVYKHECSADDGKRIAKLPWNFVDEFYK
                     WLHQKFDSDKPNDSSNNKEANTYTTIDVKR"
     gene            complement(3073..3627)
                     /locus_tag="AC04VQ_00750"
                     /pseudo
     CDS             complement(3073..3627)
                     /locus_tag="AC04VQ_00750"
                     /inference="COORDINATES: protein motif:HMM:NF014591.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
     gene            complement(3816..4463)
                     /locus_tag="AC04VQ_00755"
                     /pseudo
     CDS             complement(3816..4463)
                     /locus_tag="AC04VQ_00755"
                     /inference="COORDINATES: protein motif:HMM:NF016054.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047465 - N-acylglucosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
     gene            complement(4456..4590)
                     /locus_tag="AC04VQ_00760"
     CDS             complement(4456..4590)
                     /locus_tag="AC04VQ_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552794.1"
                     /translation="MSGYNEEVIGEIAENLNLCEAALGDDAALIGAAEYALDRLEENE
                     "
     gene            complement(4603..5430)
                     /locus_tag="AC04VQ_00765"
                     /pseudo
     CDS             complement(4603..5430)
                     /locus_tag="AC04VQ_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(5431..5613)
                     /locus_tag="AC04VQ_00770"
     CDS             complement(5431..5613)
                     /locus_tag="AC04VQ_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552795.1"
                     /translation="MLITACTKQDMGFGSGVGGFMTALCEMGIIKTNYLPEPTIPLDD
                     SEAKKVAEIVKKEGLL"
     gene            complement(5677..6351)
                     /locus_tag="AC04VQ_00775"
     CDS             complement(5677..6351)
                     /locus_tag="AC04VQ_00775"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIO9552796.1"
                     /translation="MDISKLSGVIPPVVTPLDKDKKFDKESFAKVINRMIDAGIHGVF
                     VLGSTGSVVFQTKDDREEIVRTADEIIAGRVPLLVGCIDTQTDRVIEHIKVCEKYNVD
                     AVVVTATFYALDCQDNIERHFRKLHEATKLPIFAYDLPVCVGKSLDRNMLVNLGKEGI
                     IQGVKDSSGNDVNFRYLCMTNEHYGHPLRLFSGHECVVDGALLSGADGVVPGLGNVDP
                     NVYVEM"
     gene            complement(6361..7179)
                     /locus_tag="AC04VQ_00780"
     CDS             complement(6361..7179)
                     /locus_tag="AC04VQ_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIO9552797.1"
                     /translation="MPKAKTTTTPIIELDNIKVDFKTRTGSLLHPNIVHAVDGVSLKL
                     MPGQTIGLVGESGCGKSTTANVMCGLQSVTSGHVYFKRQDVTKRTAKERKEIGRVVSV
                     VFQDPSTALNSRMSILDQLCDPMMVHKLGTKQEMEDRAYKLLETVGLPKSVMPALPGQ
                     LSGGQRQRVAIARSLALEPDAIIADEPTSALDVSVRAQILNLLTDLKAELNLSMIFIS
                     HDIQTVRYVSDEIIVMNGGKIVEQGTKEKIFKSPKDAYTKKLLGAAPSLLHPDL"
     gene            complement(7172..9187)
                     /locus_tag="AC04VQ_00785"
                     /pseudo
     CDS             complement(7172..9187)
                     /locus_tag="AC04VQ_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306326.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     permease/ATP-binding protein"
     gene            complement(9188..9592)
                     /locus_tag="AC04VQ_00790"
     CDS             complement(9188..9592)
                     /locus_tag="AC04VQ_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIO9552798.1"
                     /translation="MALPSIALAFPLMGQMTRIIRTSMVEELDKDYVRTALGAGIPLK
                     TVVAKNVLRNALITPVTVLGLRIGYLIGGAVVIEVIFNLPGMGTAILTGIQSNFTMLI
                     QGVVLVVAITFIIINIIVDMLYILIDPRIRSV"
     gene            complement(9755..10144)
                     /locus_tag="AC04VQ_00795"
     CDS             complement(9755..10144)
                     /locus_tag="AC04VQ_00795"
                     /inference="COORDINATES: protein motif:HMM:NF040248.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease family protein"
                     /protein_id="HIO9552799.1"
                     /translation="MQNLLRLIGRRLVALPLMILGVTILVFILAALNPVDQASAVLGE
                     SAGEAQLAEYRHEHGLDQPMIVQYFMYLGNLVQGDLGTFGGNNASVAEKIGQALPVTL
                     QLTFCGLIIGIIFALLLGVISALYRDK"
     gene            complement(10168..11727)
                     /locus_tag="AC04VQ_00800"
     CDS             complement(10168..11727)
                     /locus_tag="AC04VQ_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIO9552800.1"
                     /translation="MNLSRRSFIFGGAASLVGAGAVLGGCNHKIGGQAVNAGEAIKVA
                     MVDYFDKFDFVGAANPTIVSIGWHILEGLYDINPYTNETYPALADDAPVKIDDTTYEI
                     TLRDNAKYSNDAKVMAGDIINSINVAKKKESISGYLDFIRSVESAGNNKIKLNLNYPV
                     GDVLEQRLSLAKITPASASDVDKTTTPVGTGPYMITEMNGSIGGEIKFTPNANYNGPL
                     AFPENPMVWTIPADAEARAGAIKNDAVHICEDYPLDKIKEAGESGKSIEYIPGFECAN
                     FFFHTTNQVFSHKELRQAVYYAVNTERLINDKMAGHAGLVTCVLAETNKDYHPASTIY
                     TYNPDKAKELIAASGVANPQINFVADKNCWAYKFKDYVIADLEAVGFKCNVIDAEFRW
                     SDVGEKLKNIDYDVCMSTYERSLHGANADFLLNNMYYTDTFMDKRTGYANTPGNRLGE
                     IKSLLEDALGASGNELQDIYNQVFDIVAEEVVFYPFLRRDQVTAFDKTKVNGFRGLPT
                     GGMYCLETTLR"
     gene            complement(11774..13357)
                     /locus_tag="AC04VQ_00805"
     CDS             complement(11774..13357)
                     /locus_tag="AC04VQ_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIO9552801.1"
                     /translation="MSESSKLNLTRRSFILGGAAGLVGAGATAALSGCNRKKGGEGGG
                     SAELTGAIAYTATDINPIANTTVLGRSVFWHISEGLYNIDFHTFKTYNGLAALSPTKV
                     NDYTYDVILRDNAKFSNGQPVTTEDVIKSFKDNMKDEIFGSLLGFIKDVTAKDSKTVT
                     FTLNYPFENLLEKRLAIVFIYPASMSKDELKSKPIGSGPWTLKTFDGNNGGRIEFGKN
                     EYYEGKYAAPTDSMVWDCLLDNTSRTTAITDRTTLAMESVPDLNSGQITSSGATVDYV
                     QGFSTCYFMFNCMKPPFNDVRVRQAINYAINVQSLIDVQLDGHATPATSFLPKSHPNY
                     HKASIVYDYNPEKAKQLLAEAGQSNLNCTLTINNFWPKNLAPQIQSDLKAVGVNAELD
                     VKTVQWNTLAESNSILPYDFMLTPGDPSQICDDVDLLMSFWYGDNAWMNGRSCWKKAA
                     PDKWNELSTLMQRARESSSASTQQTLWNQCFDIIAENCPLLPFLHRELGTAYLSDKLT
                     NFKPIATPGLSFLGASASD"
     gene            13518..14438
                     /locus_tag="AC04VQ_00810"
     CDS             13518..14438
                     /locus_tag="AC04VQ_00810"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: protein motif:HMM:NF005121.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HIO9552802.1"
                     /translation="MANKTGVKKVAFLTAGGLAPCLSSSIGFLIDEYTKLDPSIEMIG
                     YINGYKGLLLGDSIEVTDAVRKNALVLTEHGGSPLGNSRVKLTNVDDCVKRGLVGEGE
                     DPLKVAADQLVKDGVDVLHTIGGDDTSLTAADLAAYLAKNDYNLTVVGLPKTVDNDVY
                     PIAQSLGAWTAAKEGAKYFRNVVKENSANPNMFIIHEVMGRNCGYLTGQTAIEYRKLL
                     EEINFLPEFNLTRERQEIHAVYVPEMKVDIESEAKRLAEVMKKNDCVNIFVSEGACVK
                     EIIAQMEAAGEEVPMDAFGHPRLDKVNPGE"
     gene            14535..14729
                     /locus_tag="AC04VQ_00815"
     CDS             14535..14729
                     /locus_tag="AC04VQ_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552803.1"
                     /translation="MKLIHDCCVKAVQAALAGEVGVVGQDENANSEIRACEFDRIAGG
                     KPYNLETSEFKQLLKDICQN"
     gene            complement(14885..15433)
                     /gene="pth"
                     /locus_tag="AC04VQ_00820"
     CDS             complement(14885..15433)
                     /gene="pth"
                     /locus_tag="AC04VQ_00820"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015761394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIO9552804.1"
                     /translation="MKVIVGLGNPGSEYEGTRHNAGWLALASLAPQLGANYWKTECGA
                     EVSHCKDEVFLVKPQSFMNLSGGPVKNVLDKYKLSVDELIVIHDDMDIEPGSIRVRKG
                     GNAAGHNGLKSLFEKLGTQDFVRVRIGIGRPSHNGPYTDYVLDEPKGKAKEDFEAGCT
                     LAAEATIYLLDHSLEETQQKFN"
     gene            complement(15433..16404)
                     /locus_tag="AC04VQ_00825"
     CDS             complement(15433..16404)
                     /locus_tag="AC04VQ_00825"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013978686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIO9552805.1"
                     /translation="MTGMTKSLVLFSGSANPELAQEICEKLDIKLGDEVLEQFSNGEI
                     YAKYPKSVRGADVFIVQSIAGEHLNDALMEMLVMIDAAKRASARTITAVVAHYAYARQ
                     DRKASPREPITAKLVANLLTVAGTDRIITIDLHQGQIQGFFDIPVNHLTALPILVDYF
                     EKKNFDKKNTCVVSPDLGRAKAAKKFADMMEVDLAIMHKGRPKHNQAEITALIGDVKD
                     KTCILNDDMIDTGGSVVAAVDTLIKKGAKEVYVCATHPVFSGPAYDRLLNSQIKEIVV
                     TNTIPVPEEKRGDKIKVVSVAGTFANAIKRVYSNESIAELFDPDFVL"
     gene            complement(16537..18315)
                     /locus_tag="AC04VQ_00830"
                     /pseudo
     CDS             complement(16537..18315)
                     /locus_tag="AC04VQ_00830"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012892.7,HMM:TIGR01494.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
     gene            complement(18322..19071)
                     /locus_tag="AC04VQ_00835"
     CDS             complement(18322..19071)
                     /locus_tag="AC04VQ_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012350.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIO9552806.1"
                     /translation="MKIDDVISTLKTNRNGLSISQAKSRLEKNGKNEFSKQRSTPLAL
                     RFVKQIIDPMILILLVAAVVSAVVSVANKESFADVFIILAVVIINAILGVYQETKAEK
                     SIEALQQMTAPKTYVIRDGKKCCISSSDIVVGDIVILETGDAVPADGRIIDSKLLQIE
                     ESALTGESLPINKLIDVLELKKSEDKIDLADRKNMAYMGSTVVYGRGSMVVTATGMQT
                     EMGKIAGELQNVRETKTPLQLKMFQLSKILT"
     gene            complement(19086..20825)
                     /locus_tag="AC04VQ_00840"
     CDS             complement(19086..20825)
                     /locus_tag="AC04VQ_00840"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIO9552807.1"
                     /translation="MLYTVIAAILSTVFAIFGPKYLGEATNIIVDCFSVNDGVMDYDQ
                     LGIVLLTALGLYVLSAGFTYATQQIGVIYSQRISYSLREKLSKKINKLKFSYFEDNQI
                     GDILSRFTNDVDLIGQNLSNVITSTVTALITLVGITIVMFSINVLLATVICIIIPVSA
                     IAVVIIVKISQKYFNAQQELLGTVNGVVEDCYSGHEVLKAFDATKTADNEFNYWNDRL
                     YIAGYRSTFISRLIFPVTQTTSLLGYVVVISMGAFMTIAGFFTIGDIQAFIQYVNTFS
                     KPLSQVSQVMNMAQQIAAASNRIFTFLDEEEVDDRNDEIIAMAEHELNISRGKALENK
                     QDTNDVNFEHVDFSYVEGTKILDDFNVYIPYGTKVAIIGPTGTGKTTIMKLLMRFYEV
                     DKGRITIDGIDVRFIDRESIRENIGIVMQDVWLFEGSIYDNIAFGNKDATLEKVKKAA
                     KISGADKFIEKLPGKYNFVVNESTENLSAGQLQLICIARAILADRDFLILDEATSSID
                     VATEDRLKLAIDKLMKGKTVFIIAHRLSTTKNADLILVMREGKIIEQGNHDSLIKQNG
                     LYAKMYNNQFGGA"
     gene            complement(20904..21839)
                     /locus_tag="AC04VQ_00845"
     CDS             complement(20904..21839)
                     /locus_tag="AC04VQ_00845"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIO9552808.1"
                     /translation="MIAFISYSSETIFAFFFIAAGFVMLPRADVSLGRIEEVLNHKLA
                     IDNFKQSTKYNYDEVKDDEYIKFEKVTFDYDEGKNKIFKGLDFKIKKNSINAIVGPTG
                     SGKSSVLKLLLRFYDVKSGKVSINGVDVKDYKEKDLRSIISYVPQKVNLFQGSLSENV
                     RTNPNIPINKRRVEEALRFANMGDFIDSHKDGINHVISQGAANLSGGQKQRVTIARAI
                     YEDQPILLLDDPFSALDANTEKKINSSLIKDFKDKTILVVTQRLSSVVNYDNIIFINK
                     GKVVATGTHKQLMKNCKEYKEAFEKAKASTIEDVK"
     gene            complement(21885..22580)
                     /locus_tag="AC04VQ_00850"
                     /pseudo
     CDS             complement(21885..22580)
                     /locus_tag="AC04VQ_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
     gene            complement(22680..23081)
                     /locus_tag="AC04VQ_00855"
     CDS             complement(22680..23081)
                     /locus_tag="AC04VQ_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552809.1"
                     /translation="MKVFKYIFKQKLLISIAIVLLLVESLCTFATPYVASIAIDYGIQ
                     NNGIVFATPLQIDAYTYRAGVLLADDDVKDVVRLSYDKVIDDKVYFENDVDSCHYELN
                     AIGYANIFRIEVAAVPLMSFLYDSVEDRPEF"
     gene            complement(23172..23720)
                     /locus_tag="AC04VQ_00860"
     CDS             complement(23172..23720)
                     /locus_tag="AC04VQ_00860"
                     /inference="COORDINATES: protein motif:HMM:NF046915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HIO9552810.1"
                     /translation="MKIENYIEKLSVLKNDEGVPTTADNLVFLTHSKNKEYLERDILY
                     SILDKQPKRAKAYWFVHFHVTQKPFGGNYSVESFGTDFIYRITIRLGYKEDQRLNVFM
                     TQIFNDLLQSGELPLQVATHTIYSEEQKKNLKKLGPVNIGSVKYVMIRKALIPESDLT
                     NLDKFAVSSKYKIRAYAGSPER"
     gene            complement(23760..25112)
                     /locus_tag="AC04VQ_00865"
                     /pseudo
     CDS             complement(23760..25112)
                     /locus_tag="AC04VQ_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014731.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
BASE COUNT         7872 a         5493 c         4363 g         7536 t
ORIGIN      
        1 tcgcgaggaa gatctacacc aactaaacgt gccatctaat ctactccttt atcctaacct
       61 tgacgttgct tatgacgagg gttttcgcaa attaccaaaa ccttgccatt gcgtctaata
      121 atcttgcatt tgtcacacat ttttttaact gaaggtctaa ctttcataac tactccttct
      181 acgtgctact tatacctata agtaattcgg cctctgttta ggtcataaac actaagctca
      241 acagtaactt tgtccccagg caaaattttg atgtaatgca tgcgcatctt gcctgaaata
      301 tgagccaaaa tctcatgacc attttcaagc ttaactttaa acattgcatt tggcaatgcc
      361 tcaagtactt cgccttcaag ttctattacg tcttgcttcg tcaattaaaa ctcctaaaca
      421 aattccaaaa ttaacgctta ctaaattgac ttttcttacg ggctttttta agaccatatt
      481 ttttacgctc aaccatacgg ctatcacgtg tcaaaaagcc agctttcttt aaagtctcac
      541 gatagtcacc agcttgcaaa agtgcacgag aaattccaag acgaagcgca ccagcctgtc
      601 ctgttgttcc gccaccttta atgttggcaa caatatcaaa atgagtttgt gtttcagtta
      661 cattaagagg acttaaaatg tcattgatca aaacttgacg gttaaaatat tctttaacat
      721 cgcgaccatt aacactaata tcgcccttac ctgcatataa acgaacgcga gcaacagcat
      781 tttttctgcg cccagttccg taatataagg cttcaggttt ctttgcctta gcagcctttg
      841 cctttgaagc tgttgctttg cttgctgtat ctttttttgc tgtttccttc tttgcacttg
      901 ctgttgtagt tttcttttct gcagctttct tagttgtagc ctttttagca gttgcagtct
      961 ttttagcagc agcttttgta gttttcttct cttcggtttt tgtctcagtt ttctttgttg
     1021 gcataattat taaccctcca ccttaatctg ttgtggtttt tgtgcttcat gattgtgagc
     1081 ctccccagca taaacatgaa gcttcatacc catttgacgg cctaatgtat ttttaggaag
     1141 cattcctttt acagcgtgtt caataactcg ctcagggtgt ttttccatag cctgctcata
     1201 agtttcttcc tttaaaccac ctggatgacc actatgatga atataagatt tcttcatagc
     1261 tttatcgcca gttagcttaa ctttagaagc attaataata acaacgttat cgccagtatc
     1321 tacatgaggt gtatattctg gcttattttt cccacgcaaa atatttgcag taaatgttgc
     1381 aaggcgacct aaaacttgat cactcgcatc aatcaaatac cattcacgtt taacgtcttc
     1441 ttttttcgca taatatgtct tcaccttata cctcctaaaa tgccaaggta aacgcccatt
     1501 tttgcaaatc aaaattaggc gcaaggtggt attttagcaa aaacaacctg ctaatgcaag
     1561 aactattttg tttttttgga aattttgttc agaactaatt tgcaagtaga ttatatagct
     1621 ttacctggtc ttttgcaagc aaattattaa tgtgctacaa tttcgcgcat gagcgattcg
     1681 aaaaaaagcg aaaatatcaa agaaaaagct aaagaaacta ctgcaaaagc taaagagcag
     1741 cttgattctt ttattgaaaa aaagccgatt gacaatatca agcttcctct ccctgtaaaa
     1801 atcttcgcag ttttatgcat cgttcaatgc gtaattacag cacctcttac atataactat
     1861 attgttgatt tcattcaaag cattcaagac gaaagcatcc tcagtcaaac cgcaagctca
     1921 attgtaatga cagtgctgtt tttgtcttgc accgtaatca caattgcact gcttatcgca
     1981 ataggcgtaa acatgctgct aaaccgccga agaatagctg caattctctc taacattgca
     2041 gccgtaacaa tcataatagc ttcgtttacc gaagtaatgg tcaaagggct tacaccagaa
     2101 atctatgttc aagttataac cattgttcta ctgattgcgc taaaaagtta tcttgatcca
     2161 agtctttcaa aagagcgcaa gcaacacagg catgagcgtg accagcaaaa taaaaaagaa
     2221 caagaatcag gaaaccttgg ttttcactcc gatggccgtc cgaagcttaa cttctttaat
     2281 ctcttttggc tcttctgtat tatgtgtgtt gtgggctacc tctgagaaat tatttggcac
     2341 atgacagtag ataatcctgg agtctacgaa gaacgcgcgg gctttctctt cggaccgttc
     2401 agcccaattt atggatttgg tgcctgtatt atcaccgtaa ttctaaataa gttttacaat
     2461 aaaaatttca ttattgtttt catcgttagt gcacttcttg gtgggttttt cgaattcttc
     2521 gtaagcctct atcttgagac ttgctttgga attcgctctt gagattactc tcacctaccg
     2581 cttaacatcg atggtcgtgt aagtattcgc ttctttatta tttgaggaat cattgggctt
     2641 atctgaatca aacttttgat gaagccattt gtaaaactca tcaacaaaat tccatggaag
     2701 cttcgctatt cgcttaccat catcggcact gcattcatgc ttgtaaacat ggttatgaca
     2761 ttcaacgctc tcgactgctg atacgctcga aacgctggcc gtccgccaca aacgcctctt
     2821 gaacaattct acgagcaaaa cttcaatgat gacttcatgc atcaacgctt cgaaaacatg
     2881 aagatgaatc cagactccgc catccgcggt agcgataata acaactaaag agattttgaa
     2941 tgtgcatttc tcaacggctt cgcggtatgc catagtgcgt tcgccagcaa gcgcatggag
     3001 ctgcaggggt ttcgaaatgt cacattcaaa atctcccctt atgagccgac aactcaaggc
     3061 ttcaccaatt gtttaattaa gatttagctc ttgttttaag cgaatgactg gaaggccgat
     3121 gactgtttca atttcgccct tgacctcatc tacgaaattg caagtatcat cttgaacccc
     3181 gtatgctcct gccttatcaa acggctgcga tgtttttatg tagtctcaga taacttcgtc
     3241 gctgactttg cgcatgtgta cctgcgatgt ttcgcaatag cgaataacgc tgttatcagc
     3301 gttaattatg caaactccag tataaactat atgtcaatta tctattagtt gtttaagcat
     3361 tgcaaaagct tcgttttcgt cttttggctt ctcaagaata ttgccatttt gataaacaat
     3421 tgtatctgca gcgataatgg tagaaggcac gctaacacga ggagcaacat taaatgcttt
     3481 tttcttggaa agatcaagaa cgatttcttc aggttctgtt ttatcagtaa cttcatcaca
     3541 gtctggaacc ataacttcgt attgaagacc aagctcttca agaatcgcct tgcgacgagg
     3601 tgattgtgat gcgagtataa ctttcataac ataattatta tacactttaa taaattgatg
     3661 aaaccttgag atgtcggctc atatggagag attttgaatg taacatttcg aaacccctgc
     3721 agcgccatgc acttgctggc gaacgcacga tgacataccg cgaagccgtt gagaaatgca
     3781 tattcaaaat ctctttagtt gattttattt acgaatcagc gagtgatgga agttggatga
     3841 gtgattccag tgccaacaca tacggcgaaa gccccagctt cgaaggctgc tttagcgtca
     3901 gctggagtat gaactcttcc ctcgcaaaat accggaactt ctcttgcaat tttcttcatt
     3961 tcggagatca actcaagtgc cggaccatca cttggaggat caccaggaag gttacctgct
     4021 aatgttgtag caactatgtc aactcccaaa tcaagcgcag ctttcgtatc ttcgatgttt
     4081 gaacaatcgg ccataattaa agtcttacct ttgatggctt taacggtatc agcaaatgtt
     4141 gatccatctg ggcgcgggcg caaggtcgcg tcaattgcaa caatgtcact acctgcttcg
     4201 atgcacttca gagcatggtt tacaccaggg gtgatataga catcttcgtg accagctttt
     4261 caaaggccaa taactggaac atttacgcgc ttcttaatga gttctatatc gcgaaggcct
     4321 tgacaacgaa ttgcagcagc tccaccttcg acagctgcct cggccatttg agccatggtt
     4381 tcagagcagc gcattggctc acccggatag gcttgacagc taacaattag tttgccttct
     4441 aaatctgcaa taagcttatt cattttcttc caatctatct aaggcgtatt cggcagcgcc
     4501 aattagagca gcatcgtcgc caagtgcggc ttcgcataag ttaaggtttt cagcaatttc
     4561 gcctattact tcttcgttat agcctgacat gactgcatct tttcatagtt gaccagattt
     4621 tgcaacagag ccacaaagca caatcttttc cggatcaaat acagattgat acattgcaat
     4681 aatttgtccc aaacttctgc ctgctaattc aatagcagaa actgcatctt tgtcaccttc
     4741 gttagctaat tttgaaattt caggaccact cttcttttca ccagaaaatt ttgcatagca
     4801 aaattcgata ccagtccccg aagatatagc ctcaaggtgg ccacttttgc cacaagtgca
     4861 aggaatgccg gttgccaagc acgattgtac atgccccaac tcaccagcgt atccattatt
     4921 tccaagcatt agtttgccgt ctataacagc agcgcctcca atgcctgttc caatagccaa
     4981 aagaataaag ttatcacaat ctcggccggc acctcatcga gcttcgccca ttgtatgaga
     5041 ttgaacatca tttaatgcat gtgcaggaat tgaaatagcc tcagaaacct tagcgcaaag
     5101 atcgaagcca gcaaaacctg gaaagttatc cgtagcggtc attacttttc cactattttt
     5161 gttaattctg ccagcacagc ccattccaat tgcaataact ggaacggtcg aatctgcatc
     5221 ttcggctttt gattttaggg atttcgcaag attaatgata tttgaactta atttttcaac
     5281 tccagctttg gcttcggcgg gtactttatc gtaataaaca atttcaggag cttcgtttga
     5341 ctttgcgtac ttaacaatag ctcctccaag tttagtgcca ccaatatcta tggcaagaac
     5401 gaaaaatgga tatttttgat tgtgcgtcat ttataaaagc ccttcttttt tcacaatttc
     5461 ggcaactttc ttagcttcgc tgtcgtccaa aggaattgta ggctctggca aataattggt
     5521 ttttattatt cccatctcac aaagagccgt cataaaacct ccgaccccag atccaaaacc
     5581 catatcttgt tttgtgcaag ctgttataag catttctctt gctgctttat cttgaagctt
     5641 tttaacttcg tcgaaatcct ttttattaaa cgcgtttcac atttcaacat atacatttgg
     5701 atcaacattt ccaagcccag gaacaactcc atcggcccca gaaagcaatg ctccgtcaac
     5761 aacacactca tgtccagaaa acaaacgaag cggatgacca tagtgttcgt ttgtcataca
     5821 taaatatcta aagtttacat cgttgccaga agaatctttt acgccttgaa tgattccttc
     5881 tttgcctaaa ttaacaagca tatttctatc caagcttttt cccacacata caggcaggtc
     5941 atatgcaaaa atcggaagct ttgtggcttc gtgaagcttg cgaaaatggc gctcaatatt
     6001 atcttgacaa tccaaagcat aaaaagttgc tgtaacaaca acagcatcta cgttatattt
     6061 ctcacacact ttaatgtgtt caattactct gtcagtttgg gtgtcaatgc atccgaccaa
     6121 taacggaact cgtccggcaa taatttcatc agcggttcta actatttctt cgcggtcatc
     6181 ctttgtttga aaaacaaccg aaccagttga accgagaaca aaaactccat ggatgccagc
     6241 atcaatcatt ctattaataa ctttggcaaa cgattcttta tcgaactttt tgtccttatc
     6301 aagtggtgtt acaactggcg gaatcactcc gcttaattta gaaatatcca tgctagcctc
     6361 ttataaatct gggtgaagaa gcgaaggcgc agctccaagc aactttttag tgtaagcatc
     6421 cttcggcgat ttaaatattt tttctttagt tccctgctcg acaatttttc caccattcat
     6481 gacaataatt tcatcggaaa catacctgac agtttgaatg tcatgactaa taaatatcat
     6541 tgaaagattg agctcagctt taagatcggt caaaaggttt aagatttgag cgcgaacaga
     6601 aacatccaaa gcacttgttg gctcatcggc aattattgca tctggctcaa gcgcaagaga
     6661 acgcgcaata gcaacacgtt gtctctgacc accagaaagc tgacctggaa gagctggcat
     6721 tacagacttc ggaaggccaa cagtctcaag caatttataa gccctatctt ccatttcttg
     6781 tttagttcca agtttatgaa ccatcatagg atcacaaagc tgatccaaaa ttgacatgcg
     6841 agaattaagt gcagtagatg gatcttggaa cacaacagaa acaacacgac caatctcttt
     6901 gcgttccttc gctgttcttt ttgtgacatc ttgtctttta aaatagacat gccccgaagt
     6961 aacgctttga agtccgcaca taacgttagc agttgtagac ttaccgcagc cgctctcacc
     7021 tacaagacca atggtctgac ctggcataag tttaaggcta acgccatcta cagcatgaac
     7081 gatattagga tgaagaagcg agcctgtgcg tgtcttaaaa tcaaccttga tattatcaag
     7141 ctcaataatt ggcgttgttg tagtcttggc cttaggcact tttcttacct ttcacatatg
     7201 gtgtaatctt caaacgcttt aattcttcgt ctggaagttc cgaataaaaa tgtaagtcct
     7261 tcttgtattg cttcaaaatt ggcaccacat cagatttaac atccggatga cttgaccttg
     7321 gagcgaagcg gtctcccttc gggaaatcct gaggacttgg aacagctcca acaatttggt
     7381 gaagtcttcc agacccgctt tcgattgaaa caactgaacc caaaagccca cgagtgtatt
     7441 cgtgaattgg gtttgtcata atatctttaa ctgaaccagt ttcaacaact tgaccagcat
     7501 acataacagt tacttcgtta gcaacttcgg ccacaagcgc taagtcatga ctaacaaata
     7561 tcatagcgaa gccaagcttc ttctgcaaat ctgacaacag tttaatgact tgcttctgta
     7621 ctgtaacatc aagtgcagtt gtaggctcat cggcaataat aagttttgga tcacgagtaa
     7681 gagccatagc aatcaaaaca cgttgacgct gaccgccaga taattcatga ggatacgaat
     7741 ccaaagttct ttgcggatca agaccaacaa gttcaagcag ttcttcggca cttcgcttgc
     7801 ctccacgcat tgtcaattgc ttcatttgag attttataag catcgaagga ttaagcgagc
     7861 ttagcgcatc ttgataaatc atagcaatgt ccttgccacg aagctcctgc atttgctcat
     7921 cttttaagtc aagcaaattc tgattgtcat acataacttt tccagaaatc tttgcctttt
     7981 tatcaagcag tcccataatt gaaagcgaag taatagattt gccgcaacca ctctctccta
     8041 caagacccat tgtttgcccc tcgcgaacaa cgaagttaat attttctaca acgttaacct
     8101 taccatggcg aggaaattga atgcacaaat tctcaacact tagcacaggc tttttcttaa
     8161 gatcaggagt caacctatct ttgcgtttaa gttcaacctt acgaagctca gccaacctct
     8221 tgtctaaaag tttattttga gcttcaactt cttttttgtc taaagtaata acttgttttg
     8281 actcaactgc agacatatgt agaggcttag tttttggact tgccaaagta tctgtcatgc
     8341 cttcagccaa aatattaaat gtcaaaactg tcagcatgat tgcaagacct gggaagcatg
     8401 cctgccacca tctaccagat aaaacaccat ctcgagcatc agctaaaatg tttcctcaag
     8461 taggtgttgg ctcaggaatt ccagcactga tgaaagaaag cgaggcctca aaaacaattg
     8521 catcagcaat caaaacaatt gtaaaaacta aaattggagc agcacaattt cgcgcaacat
     8581 gtttaaacaa gatccaagga ctcttcgcac cactaacaac tgcagcacgc gcataatcct
     8641 gattgtattc gctcataata ttcgctcgaa caattctggc aatttgagga atatataaaa
     8701 tacctatcgc aaaaacaatt gacggaagtg aatttccaaa agtcaaaaca aaaatagctg
     8761 ccaatgcaat tcctggaaaa gacataaaaa cgtccatgat tctcatgatt acttcgccaa
     8821 ttcacctgcg cgaaacagca gctatcgagc caataattga acctataatt agagcaaaag
     8881 cagtagctcc taaaccaatt actaaggagt atctgccacc aaaaagaata cgggataaaa
     8941 catcgcgacc cttatcatcc gttccaaata aaaattcaaa cgatgggggt cttcttgcta
     9001 agaaaatctc caatgggtca tatggagcta aaatatcagc aaaaatagtt gcaagaacaa
     9061 taactaccaa aacaattaaa gagattttcg aacttctatt taagctttta aaagcttgaa
     9121 ctataccaat attgttcccg gcagagctca ctaacttatc cgttgttttg cctcgtagcc
     9181 taaacatcta cacactccta attcgtggat caataagtat gtagagcata tcgacaatga
     9241 tattaattat gataaacgtt atggcaacaa ccaaaacaac accctgaatc agcatcgtaa
     9301 agtttgactg tataccggtc aaaatcgcag tacccattcc aggcaaattg aatataactt
     9361 cgatgactac agcgccacca atcaaatatc caatacgaag ccccaaaacg gtaaccggag
     9421 ttataagcgc attgcgcagc acgtttttag caacaacggt cttaagcgga ataccagctc
     9481 caagtgcagt tctaacgtag tctttgtcaa gttcttcgac catgcttgtg cggataattc
     9541 tggtcatctg acccattaaa ggaaatgcta aagcaataga tggtaatgcc attcgagcac
     9601 atcatcgccc ggggtcgtca aagaaattcg gcaactgtcc agaagccgga agaatatgag
     9661 tgttagctga aaataccaaa atcaataaaa cagctaatca aaacgaaggc gtagcaattg
     9721 ccgctattga aattacacga attgtttgat cttgtcattt atctcgatac aaggccgaaa
     9781 ttacaccaag caacaaagca aaaattatgc cgataattaa accgcaaaaa gtaagttgca
     9841 atgtaacagg aagtgcctgc ccaatttttt cagcaacaga agcgttgttt ccaccaaatg
     9901 tacctaaatc accttgaact aaatttccaa gatacataaa atattgaaca atcataggct
     9961 gatcaagccc atgttcatgt ctatattcgg ctaattgtgc ctctcctgca gattcaccta
    10021 aaacagcaga agcctgatca acagggttaa gagcagctaa aataaaaact aaaatggtaa
    10081 cgccaagaat cattaaaggg agcgcaacta atcttcgtcc tatcaacctt aataaattct
    10141 gcacagcact cccaatctat ataaaatcta tcttaaagtt gtttccaagc aatacatgcc
    10201 accggtaggt agacccctga atccattaac ctttgtttta tcaaaggctg taacctgatc
    10261 tcttcgcaaa aatgggtaga acactacttc ttcagctacg atgtcaaaaa cttgattgta
    10321 gatatcttga agttcatttc cgctcgcacc taaagcatct tctaataaag atttaatttc
    10381 acccagtcta ttaccagggg tattagcata ccctgttctt ttgtccataa aagtatctgt
    10441 gtaatacatg ttgtttaata agaagtcggc attagcacca tgtaaagaac gctcgtaagt
    10501 tgacatacaa acatcatagt caatattctt tagcttctct cctacatcgc tccatctaaa
    10561 ttcagcgtca ataacattac acttaaaacc tacagcctca agatctgcaa tgacataatc
    10621 cttaaactta tatgcccaac agtttttatc agcaacaaaa ttaatttgag gattagcaac
    10681 accacttgca gcaataagtt ccttcgcttt gtcaggattg tatgtataaa ttgtggaagc
    10741 tggatgataa tctttgttgg tctcagccaa aacacatgtg actaaaccag catgaccagc
    10801 cattttatca ttaatcaaac gttcagtatt aaccgcataa taaacagcct gacgaagttc
    10861 cttgtgagaa aaaacttggt ttgttgtatg gaagaagaag ttcgcacact caaaaccagg
    10921 aatatattca atagattttc cgctctctcc agcttcctta attttgtcaa gaggataatc
    10981 ttcgcaaata tgaacagcat catttttaat tgctccagcc cttgcctctg catctgcagg
    11041 aattgtccac accataggat tttcaggaaa agcaagtgga ccattatagt tggcatttgg
    11101 tgtaaattta atttctccac caatactacc attcatttcg gtaatcatat aaggaccagt
    11161 tccaacagga gttgtggtct tatctacatc ggaagcagaa gccggtgtaa tttttgcaag
    11221 acttaatctc tgttctaaaa catctccaac aggatagttt aagttaagtt taattttgtt
    11281 attaccagca ctttcaacgc ttcgtataaa atccaaataa ccagaaattg attctttttt
    11341 cttggctacg ttaattgaat tgatgatatc ccctgccatt accttcgcat catttgaata
    11401 ttttgcatta tcacgaagtg taatttcata ggttgtatca tcaattttta ctggagcatc
    11461 atcagccaaa gctggataag tctcattagt ataagggttg atgtcataaa gaccttcaag
    11521 gatgtgccat cctatagaaa caatggttgg attagcagcg cctacaaagt cgaatttatc
    11581 gaagtaatct accatggcaa ctttaatagc ttcgccagca ttaactgcct gtcccccaat
    11641 tttgtggtta cagccaccta aaacagcgcc agcaccaacc aaacttgcag caccaccaaa
    11701 aataaaacta cgacgactta aattcataat taccctctct caaaattatc accacataaa
    11761 gtggattact aaattagtcc gaagccgaag ctccaaggaa cgaaagacct ggagttgcaa
    11821 taggcttaaa gttagtcaat ttatctgaaa gataagcagt tccaagttca cgatgcaaaa
    11881 atggcaacaa tgggcaattt tcagcaataa tatcgaagca ctgattccac aatgtctgct
    11941 gagttgaagc acttgaactt tcacgagcac gctgcataag tgtactaagc tcgttccatt
    12001 tatctggagc agcctttttc cagcatgacc taccattcat ccaagcattg tctccatacc
    12061 aaaatgacat tagcaaatct acatcgtcac aaatctgcga tggatcacca ggtgttagca
    12121 tgaaatcata tggaaggata gaatttgatt ctgccaaagt gttccactga actgtcttaa
    12181 catctaattc tgcattaaca ccaacagcct tcaaatcgct ctgaatttgt ggagcaagat
    12241 tcttaggcca gaaattgttg atagtcaatg tgcaatttaa attactctgt ccagcttcgg
    12301 cgagaagttg ttttgccttt tctggattgt aatcataaac tattgaagcc ttgtgataat
    12361 ttggatggct cttaggtaag aacgaagttg ctggtgtagc atgtccatcc aattgaacat
    12421 caatcaagct ttgaacatta attgcataat tgattgcctg tcgaacacga acatcattaa
    12481 acggaggctt catgcaatta aacatgaagt agcaagtact aaagccttga acataatcaa
    12541 ctgttgcacc ggaagacgtt atttgtccac tgtttaagtc tggaacagat tccattgcta
    12601 aagttgttct gtcagtaata gcagtagttc ttgatgtgtt atcaagcaag caatcccaaa
    12661 ccatcgaatc ggttggagca gcgtactttc cttcgtaata ttcatttttg ccgaattcaa
    12721 ttctgccgcc attattacca tcgaaggtct taagagtcca aggaccagaa ccaataggct
    12781 tagattttaa ttcgtcctta gacatgcttg caggataaat aaatacaata gcaagacgct
    12841 tttcaagcaa attttcaaat ggataattta gtgtaaatgt aacagttttt gaatctttag
    12901 ctgtaacatc cttaataaaa cctaatagcg aaccaaagat ttcatccttc atattatcct
    12961 taaatgactt aataacatct tcggttgtaa ctggttgccc attagaaaac ttagcattat
    13021 cacgtaagat gacatcataa gtataatcgt taacctttgt tgggcttaaa gctgctaatc
    13081 cattatatgt tttaaaagta tgaaaatcta tattgtaaag gccttcagaa atgtgccaaa
    13141 atacagatct tcccaaaact gttgtattag caattgggtt aatgtccgtg gctgtataag
    13201 caattgctcc tgtaagctct gcgctaccgc cgccttcgcc gcctttttta cgattacatc
    13261 cagacaaagc agcagtcgca ccagcaccaa caaggccagc cgcacctccc aaaataaagc
    13321 ttcttcttgt caaatttaat ttgcttgatt cagacattct tacctccaaa taataatcac
    13381 aaatacaggc agaattatac aataaaaggg caaaaatttg accgccaaaa ttataatttt
    13441 tctgggtttt atttcaataa ataattataa aattacatca cgaatcttaa tgaaattttt
    13501 taacttaagg agtaaaaatg gctaacaaaa caggagtaaa aaaagtagct tttctaacgg
    13561 ctggtggatt ggccccttgt ctttcaagct caataggctt tttgattgac gaatacacaa
    13621 agcttgaccc atccattgag atgattggat acattaatgg ttacaagggt cttctccttg
    13681 gtgattctat cgaagttacc gatgccgttc gcaaaaatgc tcttgtttta accgagcatg
    13741 gtggaagtcc gcttggaaac agccgagtta aactcacgaa tgttgatgat tgcgttaagc
    13801 gcggtcttgt tggcgaaggc gaagacccac tcaaggttgc tgctgaccaa ctagttaaag
    13861 atggcgttga tgttcttcac acaattggcg gcgatgacac ttcgctgact gcagcagact
    13921 tggccgcata tcttgccaag aatgattaca acttaactgt tgttggtctt ccaaaaacgg
    13981 ttgataatga cgtctaccca atcgctcaat ctttgggtgc ttggactgcc gcaaaggaag
    14041 gtgctaaata cttccgcaat gtggttaagg aaaattctgc aaatcctaac atgtttataa
    14101 tccacgaagt tatgggtcgc aattgtggct atctaacagg tcaaacagca attgaatacc
    14161 gaaagttact agaagaaata aacttcttac cagagtttaa cctcacacgt gagcgtcaag
    14221 aaattcatgc agtctatgtt ccagaaatga aggtcgatat cgaatcagaa gccaagcgtc
    14281 tcgctgaagt tatgaagaaa aacgattgcg ttaacatctt cgtatccgaa ggcgcatgtg
    14341 taaaagaaat catcgcgcaa atggaagctg ctggcgagga agttccaatg gatgcttttg
    14401 gtcacccgcg acttgataaa gttaatccgg gtgaatgatt tggaaagcaa tttgcatcca
    14461 agattggtgc agaaaaagtt cttgttcaaa aatctggcta ctattctcgc gctgctgctc
    14521 cttgcgaaga agatttgaag ctcattcatg attgttgtgt aaaggctgtg caagcagctt
    14581 tagctggtga agttggggtt gttggtcagg acgaaaatgc aaatagcgaa atccgcgcat
    14641 gtgaattcga ccgcatcgct ggcggcaagc catacaatct agagacttca gaatttaaac
    14701 aactcctcaa agacatctgt caaaattaag tctttttaga gagatttcga atatgtattt
    14761 ctcaacggct tcgcggtatg ccgtcgtgcg ttcgcctgct ataaataggc gctgcagggg
    14821 tttcgaaata tcacattcga aatctctcac ttatgagccg acaactcaaa gcttcaccaa
    14881 ttatttaatt aaatttttgt tgggtctctt ccaaagaatg atctagaagg tagattgtgg
    14941 cttcggccgc cagcgtgcag ccagcttcga agtcctcttt cgctttgcct ttaggctcgt
    15001 ctaaaacgta atctgtgtat gggccgttgt gcgaaggtct gcctattcca attcgaactc
    15061 ggacaaagtc ttgagttcca agcttttcga acaaagattt cagaccattg tgtcctgcgg
    15121 cgtttccgcc ttttcttaca cgaatcgagc caggctcgat gtccatatcg tcgtgtataa
    15181 caatcaattc gtctaccgac aatttgtact tatccaaaac attttttact gggccgccgg
    15241 acaagttcat gaagctttgg ggctttacta aaaagacttc gtccttgcag tgagaaactt
    15301 ccgctccgca ctcagttttc caatagtttg cgcccagttg cggagcgagg ctcgctaatg
    15361 cgagccagcc agcattatgc cgagtacctt cgtactcgct acctgggtta cctagtccga
    15421 cgattacttt cattacaaaa cgaagtctgg gtcgaagagc tcggcgatag attcgttaga
    15481 ataaactctc ttgattgcat ttgcaaatgt gcccgcaaca gatacaactt taatcttatc
    15541 tccgcgcttt tcttcaggaa ctggaattgt attagtgaca acaatctctt taatttgcga
    15601 attgagaagt ctgtcatatg caggacccga aaatactggg tgtgtcgcac aaacataaac
    15661 ttccttcgca ccttttttaa taagcgtatc aactgcggca acaacacttc cgcctgtgtc
    15721 aatcatgtca tcatttagaa tgcaagtctt atccttaaca tcgccaatga gcgctgtaat
    15781 ttcagcttga ttatgcttcg gacgaccctt gtgcataatt gccaaatcaa cttccatcat
    15841 gtcagcgaat ttcttcgcag cttttgcgcg accaagatcc ggactaacaa cgcaagtatt
    15901 tttcttatca aaatttttct tctcgaaata gtcaactaaa attggcagag cggttaaatg
    15961 attaaccgga atatcgaaga agccttggat ctgaccttgg tgcaaatcaa tcgtaatgat
    16021 tctgtcagtg cccgcaacag ttaaaaggtt agcgacaagc ttcgctgtaa ttggttcacg
    16081 aggcgaagcc tttctatcct gtcttgcata tgcataatgc gcaacaacag cagtgatagt
    16141 tcttgcactt gcacgcttcg cggcatcaat cataacaagc atttccatta aagcatcatt
    16201 taagtgttcg ccagcaattg attgaacgat aaaaacatcc gctccgcgaa cagactttgg
    16261 atacttcgca taaatttctc cgttagagaa ctgctcaaga acttcatctc ctaatttgat
    16321 gtctaatttt tcacagattt cttgtgctag ctcggggttt gcactccctg aaaataggac
    16381 taaagactta gtcattccgg tcattttgtc tccttcgttt ggatttcctg ccggaattcc
    16441 ataataacat attcacaatt ttatttaatt aaaaattata aatttttaaa gaaaaattaa
    16501 cgtggaatga aacgccaatg cccccgtccc tatttactat ttattgattc taaagtttat
    16561 agattttctt tgtataaatt taacaatttc gacgattggg atgatggcaa gcccaagact
    16621 aagagcaata ccccattcag taactccaag actggtaagt ttgaaggctt cggcaattgg
    16681 cggaacctcg caaacaattg ttgtcaaaat taaagctaca ccaacagaag ccataagagt
    16741 ccaattccta ctctttaatg cgaagaggga ctggcggcgg cttcgtagat taattgagtg
    16801 aaaacagcca accatgcaca tcgttaggaa tgccatagaa attccgtggt ccgaagtagt
    16861 aatttgtcac gagcctgtat caataaaaac accaacaaaa tacgaaaaca taataagagc
    16921 tgtgataatt atgccctgat aaattatatc gaagcccatt ccgtccgcaa aaataccatc
    16981 agtttttgga cgaggcttac gttgcataat ttttggttct ttttcttcga gcccaagagc
    17041 caaagcagga acacaatcgg ttaccaagtt tacccacaaa agatgaacag gaagcatcaa
    17101 cgtaaaacca aacagcgaag cgaagaacac tcccagcact tctccaatat ttgaacttag
    17161 taaatactga atgcacttaa taatattttc ataaatgcgg cgaccctctt ctacagcatt
    17221 aacaatagtg gcgaagttat cgtcactcaa aaccatatca gcgacgttct ttgtaacatc
    17281 agttccggtg atgcccatgc caacaccaat atcagcagtc ttaattgaag gagcgtcgtt
    17341 aacaccatca cctgtcatgg caacaacata gccgacattc tgccaagcct taacaatacg
    17401 agttttatgc tccggacgaa ctcgcgcata cacacttata ttttgaaatt cacgagcaaa
    17461 ttcttcgtca ctcatatcat caagttgcaa accactaaca gcattgcaat cgccgctcaa
    17521 tatatttagc tcgcgtgcaa tcgcgcaagc cgtattaata tggtcccccg taatcatgat
    17581 aggcttaatt ccggcgcgtt cacatttagc aattgcatca gcaacttcgg ggcgtacagg
    17641 atcaatcatt cccacaagac cgataaatgt catttcatct tcttcaatgc tatcagcttc
    17701 gcgataagca caagctaaaa cacgaagcgc tttgtccgcc atcgatttgt tggcctccaa
    17761 aattttcgcc tcatctacat ttatgcaatg cttcaaaatc acatctggcg cacccttagt
    17821 gtattgaatt gttttaccgt ctacttcgtt tagcgtcgac atcattttgc gcttcgaatc
    17881 aaaaggcaac tcagaaattc ttgcatattt tgatttatcg atcgaggctc attcaaaaag
    17941 tgctttctct gttggctcac cactatcaac agttgcatca ttgcaaagac ccatcgctcg
    18001 cttgagcatt ttttcatctc cgaagaaatc tgtaacagtc atcttgtttt gagttaaagt
    18061 cccagtttta tcagaacaaa taatttgagc gcaacccaaa gtttcaacag cagtgagttt
    18121 tcgaatgatt gcgtttcgct tcgacatttt tgaaactcca atagaaagag caacggtaac
    18181 aacagcgggc agtccttcag gtattgcagc gaccgctaaa gaaattgcaa tcataaacga
    18241 agaaattata acttcggtgc tcataacacc cgcactaagt aactgtaatc caaatacaac
    18301 aagactaatt atcacaacaa ctcaagttaa gatttttgaa agttgaaaca tcttaagttg
    18361 aagcggagtt tttgtctcac gaacattttg aagctcaccg gcaatctttc ccatttcagt
    18421 ttgcatgcca gttgcagtta caaccataga gccacgacca taaacaacgg tagaacccat
    18481 ataagccata tttttcctgt cagcaagatc aatcttgtct tcggattttt taagttctaa
    18541 cacatctatt aatttattaa ttggaaggct ctcacccgtc agcgccgatt cttcgatttg
    18601 taaaagtttc gaatcgataa tgcgtccatc ggcaggcacg gcatcgccag tttctaaaat
    18661 tacaatatcc cccacaacta tatcagacga agatatgcag cacttcttcc catcgcgtat
    18721 gacataagtc tttggagcgg tcatctgttg aagcgcttca atagattttt cagctttggt
    18781 ttcttgatag acacccaaaa tcgcattgat aattacaacg gccaaaatga taaacacatc
    18841 ggcaaacgat tccttattgg caacagaaac aactgctgac acgactgcag caacaagcaa
    18901 aattaaaatc atcggatcta tgatttgctt aacgaagcga agcgcaagcg gcgttgacct
    18961 ttgcttcgaa aattcattct ttccgttctt ttcaagcctt gactttgctt gagaaataga
    19021 caaaccattt ctatttgttt taagtgtaga aataacgtcg tctattttca ttaggtgaaa
    19081 atccactaag caccgccaaa ttggttgtta tacatttttg cgtacaatcc attttgttta
    19141 attagcgaat cgtgattacc ttgctcaata attttacctt cacgcataac caaaatcaaa
    19201 tcagcatttt ttgtagtcga caacctgtga gcaataataa atacagtttt gcctttcatt
    19261 aatttatcaa tggcaagttt gagtctatct tcggtagcaa catcaatgga gcttgtagct
    19321 tcgtcaagaa ttaagaaatc acggtccgcc aaaattgcac gagcaataca aatcaactgc
    19381 aactgtccgg cgcttaagtt ctcagtcgat tcattaacaa cgaagttgta cttgcccgga
    19441 agcttttcta taaacttatc agcgcccgaa atcttcgctg ctttcttaac cttttccaaa
    19501 gttgcatcct tgtttccaaa agcaatgttg tcataaatgc ttccttcgaa aagccaaaca
    19561 tcttgcatta caattccgat attttcacga atactctccc tgtcaataaa gcgaacatca
    19621 ataccgtcaa ttgtaattcg gcctttatca acttcgtaaa aacgcatgag caatttcatg
    19681 attgtagttt tacccgtacc agttggccca attattgcta cctttgttcc ataaggaata
    19741 taaacattga aatcatcaag aatctttgtt ccttcaacat aggaaaagtc aacatgttca
    19801 aagtttacat cgttagtgtc ttgtttgttt tctaatgcct tgcctcgcga aatattaagt
    19861 tcatgttcag ccattgcaat tatttcgtca tttctatcat caacttcctc ttcgtcaagg
    19921 aaagtaaaaa tacggttcga agccgcagca atttgctgag ccatattcat aacttgacta
    19981 acttgcgaaa gcggtttgct aaaagtattt acatattgga tgaaggcttg aatgtctcct
    20041 atggtgaaaa atcctgcaat ggtcataaac gcacccatag aaataaccac aacatagccg
    20101 agcaaacttg tagtttgagt gacagggaaa atcaaccttg aaataaacgt agatctataa
    20161 cctgcaatat acaatctatc attccaatag ttaaattcat tgtctgccgt tttggtagca
    20221 tcaaaagctt tcaaaacttc gtggccagaa tagcaatcct caacaacacc attaacagta
    20281 ccaagcaact cttgctgtgc gttaaaatat ttttgcgaaa ttttaacaat gatgacaacc
    20341 gcaattgccg aaactggaat gattatgcat attactgtag ccaaaagcac attgatgcta
    20401 aacataacaa tcgtaatacc gactagtgta attaatgctg taacggtact tgtaataacg
    20461 ttagatagat tttgtccaat taggtcaaca tcatttgtaa accttgataa aatatctcca
    20521 atttgattgt cttcaaaata tgagaatttt aacttgttaa ttttttttga aagtttttct
    20581 cttaagctat atgaaattct ttgagaatat ataacgccaa tttgctgtgt ggcataggta
    20641 aagccagcag aaagaacata caaaccaagt gccgttagta aaacaatacc aagttgatca
    20701 taatccataa caccatcatt aacagaaaaa caatcaacaa taatatttgt agcctctcct
    20761 aaatacttag gcccaaaaat tgcaaatact gtagacaaaa ttgctgcaat aactgtataa
    20821 agcaatagat gtcaattctc aagaagcaaa cgaattaagt tcttgaagat gtgttgcggt
    20881 tgtattttaa tatccttctt cacttatttc acatcctcaa ttgttgaagc ttttgctttt
    20941 tcaaacgctt ccttatattc tttgcaattc ttcataagtt gcttgtgggt tccagttgct
    21001 acaactttgc ctttattaat aaatatgata ttgtcgtaat taacaactga acttaaacgc
    21061 tgtgttacaa ctaaaattgt tttatctttg aaatctttta tcaacgaaga attaatcttc
    21121 ttttcggtat ttgcgtccaa agctgaaaaa ggatcatcca aaagtaaaat tggctggtct
    21181 tcataaattg cacgagcaat tgttacgcgc tgtttttgtc cgccagaaag atttgcagca
    21241 ccctgactaa taacatggtt tattccgtct ttgtgcgaat caataaaatc acccatattt
    21301 gcaaaacgaa gcgcctcttc gacccttctc ttattaattg gaatattcgg attagttcga
    21361 acattttcgc ttaatgaacc ttggaacaaa ttaacttttt gcggaacata acttataatg
    21421 cttcgcaggt ctttctcttt atagtcttta acatcaacac cattaatact aacttttcca
    21481 gatttaacat catagaaacg aagcaacagc ttcaacacgc tagatttacc tgagcctgta
    21541 ggcccaacaa tcgcattaat agaattcttc tttatcttaa agtcaagacc cttgaaaatt
    21601 ttattcttgc cttcgtcata atcaaatgta actttttcaa acttaatgta ttcatcatct
    21661 ttaacttcat cataattata ttttgtactt tgcttaaagt tatcaatagc aagtttatga
    21721 tttagcactt cttcaattct gccaagagaa acatcagcac gcggaagcat tacaaagccc
    21781 gcagcaataa agaagaaagc aaaaattgtt tcagaagaat aagaaataaa tgcaatcaat
    21841 tgaccgatgt ttactaaacc attttgaagt tgaaaacttc cttctcataa aattgctacc
    21901 gacaaaatat ttacggttag cataagaata ggcatagcaa taaccaaaat tgacatcgca
    21961 aatgtcatcg tgtggtataa cttcttgtta acttcgctaa acctctcctc ctggaaattt
    22021 tcattgttaa gagcacgaat aattggaacg ccagaaattg attcgcgagc aaccgaatta
    22081 atcttatcaa caagtttttg aatcagccta tactttggaa atacaatcgc tattaatact
    22141 gcagcaacta caagtattgc gccaatagta acagctggca atcatacaag tgaaggtgca
    22201 gtagtatatg ccatgaaaaa tcctacaatc atcataactg gagctattaa tattgccctt
    22261 ataacaaaca cgcttgtatt ttgcacgacc tgaagatcgt tagtagcacg agtaattagc
    22321 gaggcgcgcg aaaacttatc catatcatga tttgaaaagg acataacttt tgcaaattgc
    22381 tttttacgta agtctctaga tatagtaaaa gaaattttcg aagctaaata agttccaata
    22441 atacaagata tcaaatacaa aacaacaatt atcaacatac tcaaagaaat gaataccata
    22501 aaattcactt gataccattc attatcaaaa cctaaatatt cgttttcaag aagctttaac
    22561 ttaacggctt tattgtacat gtcagaaaag tcatgattat ttttaaattc ctgtacttta
    22621 ttcctaaaat cgttaatttc agcaacagta atatcacctt gatttacctt acctaaacgt
    22681 cagaattctg gcctatcttc aacgctgtca tataaaaaag acattaaggg aacggctgcg
    22741 acctcaattc taaaaatatt agcgtaacca atagcattta gctcataatg acaactgtca
    22801 acatcatttt cgaaatagac tttatcgtca ataactttgt cgtaagataa acgaacaaca
    22861 tcctttacat cgtcatcagc aagcaagacg cctgctctat aagtataagc atcaatttgc
    22921 agtggcgtgg caaaaacaat cccgttgttt tgaataccat aatctattgc aatggaagca
    22981 acgtaaggtg tcgcaaaagt acacaaggat tcaaccaaaa gcaaaacaat cgcaatgcta
    23041 attaataact tttgcttgaa tatgtattta aaaactttca cttactttcg ctccagcggt
    23101 ttgtcgaccg gcttcgcttc aataaataac ggaacgtatt ctattaataa cgaagagttt
    23161 tccaagccaa atcaacgctc aggagaccct gcataagcac gaattttata tttagaagac
    23221 acagcaaatt tatccaagtt tgtaagatca ctttcaggaa taagggcttt gcgaatcatt
    23281 acatatttaa cacttccaat atttaccgga ccaagtttct ttaaattttt cttttgctct
    23341 tccgaatata ttgtatgagt tgcaacttga agcggaagtt ctccggattg caacaagtcg
    23401 ttgaaaattt gagtcataaa aacattaaga cgttgatctt ctttataacc caaacgaatt
    23461 gtaattctat aaataaaatc agttccaaaa ctttcgactg aataatttcc gccaaaaggt
    23521 ttttgcgtta catgaaaatg aacaaaccaa tacgctttcg cacgcttagg ctgcttatct
    23581 aaaattgaat atagaatgtc gcgctcaaga tattccttat tttttgaatg agttaaaaat
    23641 acgaggttat ctgccgtagt tggaacgcct tcgtcatttt taagcacact taacttttca
    23701 atataatttt caattttcaa agttttgctc tgcttgcgtt cgaccttagt gcctcgctct
    23761 catgaaatca taacaatcaa aattaatgct gcgattaata tagcgaagta acctccatga
    23821 aaaaactttc caatgcaaga taagaaaaat acaatttcaa taaagccaaa cacaatagca
    23881 aacaaagcag ccaggaattt tttattcttt tcaacgccaa tataagccgt caataaaatt
    23941 gtaaccataa gtaaagttat agtaattgca agtccatatg ctgcttccat atttgctgaa
    24001 gtttgaaaga gcaaaacaac aattgtgcat cccgctcaaa tcaaattatt tacaagagga
    24061 atataaattt gacccttagt ttctgaaggg taaaaagttt tcatatgagg caaaagatca
    24121 agcctaatag cttcagatac aatgctgaaa gatcctgtaa tcaaagcttg cgaagcaatc
    24181 acggcagcca ttgctccaag aataattcca gcaattcgaa gctctggaga aagcatcaaa
    24241 tagaatgggt tcaaatcttc gatgcttgcc aaagatgtat ttgatgaatt agaaattatc
    24301 caagccccct gaccaagata atttagaatc aaggcaattt ttacaaacgg ccaactaata
    24361 tatatgcttc tcttgcccac atgacccatg tcgctgtaga gcgcttcggc acctgttgtt
    24421 gccaaaaaaa cacttcccaa aatcatgatt ccaactttgt tgttcgggct gatcaaaact
    24481 tcaacagcat aaagcggatt aattgcgcga ataacttcca agtcacttgc cattgcaatt
    24541 attccaccaa gagccaagaa caaaaaccaa attgtcatga atggaccgaa ggttctacca
    24601 ataatgcttg ttcctgctcg ctgcatgata aacaaaattg ctaatgtaat aacaacaata
    24661 acaataattg ggacttgtcc cggaccaatt aaattatcaa ttacatcaat gcttcgaaga
    24721 ccctcaaccg cagttgtgac tgttacggca ggcgttaaaa cactatcagc aagaagcgct
    24781 gcaccgccaa tcatcgctgg cacaataagc cattttgcac aatgtttgac taaactaaaa
    24841 agagcgaaga tcccgccttc gccgtgattg tctgccttca ttgcaattaa aacatgcttt
    24901 acagttgtta aaagcgtaat tgttcaaata accaaagaca aagagccaat aatgaagtct
    24961 ttatcgacat tgttaatacc tccattacca gcaacaatag acttcattac atacattgga
    25021 gatgtggcaa tatcgccata aacaattccg acggcgatta aaatcatccc cgccgaaagc
    25081 ttcgctttat tctgtttcaa tacacgcgac atagatgctt tattttacca ctaacagcat
    25141 atatttatat atatttaatt atgtattgac aaggtcagtg agaagcatta tttaattgca
    25201 tatttgcccg cctttttgac gacgtgtgct atgcacacga gtcaaaaaac aagggtaaag
    25261 atgc
//
LOCUS       DBNCDC010000005        22335 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_546_length_22335_cov_6.647038, whole genome shotgun sequence.
ACCESSION   DBNCDC010000005 DBNCDC010000000
VERSION     DBNCDC010000005.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 22335)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 22335)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22335
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_546_length_22335_cov_6.647038"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(30..986)
                     /locus_tag="AC04VQ_00870"
                     /pseudo
     CDS             complement(30..986)
                     /locus_tag="AC04VQ_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014841.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
     gene            1136..1720
                     /locus_tag="AC04VQ_00875"
     CDS             1136..1720
                     /locus_tag="AC04VQ_00875"
                     /inference="COORDINATES: protein motif:HMM:NF018446.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HIO9552811.1"
                     /translation="MIKKSRVEAFSDGVLAIVATIMVLELVLPGGVAWGDFAAQWPVL
                     LAFVISFFQIYINLYNHNKLFGKIECVGRHVYLLNGLWLLFACFVPFTVHYLGHNPDA
                     TLPMLIYLVNLLLWAISFQILDITILHENPKAAKDESTTIITRIIYYVGLFIAGITAI
                     FIPRISLFIILIVVAGEIITTFFKATKTHDEACK"
     gene            complement(1903..2349)
                     /locus_tag="AC04VQ_00880"
     CDS             complement(1903..2349)
                     /locus_tag="AC04VQ_00880"
                     /inference="COORDINATES: protein motif:HMM:NF015325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIO9552812.1"
                     /translation="MKVLMINGSPHEDGSTFTALSEMKKVFDAEGVEAEIVQVGHLDI
                     PGCKACGYCYKAGKCKFDDIVNEILPKVEEADGLVVGAPVYFASSNATLNAFMDRLFY
                     VMLRKDVRMKVGACVVSARRGGLTSSFDQLNKYFTIAGMPVASSQY"
     gene            2531..3529
                     /locus_tag="AC04VQ_00885"
     CDS             2531..3529
                     /locus_tag="AC04VQ_00885"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF014177.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032775 - obsolete DNA methylation on
                     adenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HIO9552813.1"
                     /translation="MNYIGSKYSLIDFLVESIDDVTGFNSGDGRTFADLFAGTGIVGA
                     TFKERGYKVISNDIQYYSYVINKHLIENCKEYGNSKINDLNNIKFKEGFIYKNYCSGS
                     GSGRNYFTDDNGKKCDAIREEIDNLKTNKKISNGEYYYCLASLIDSVDKCANTASVYG
                     AFLKHIKESAKKDLELRPLPTINGPKGKVYNEDINILIKRINGDILYLDPPYNSRQYC
                     SNYHLLETIAKYDNPEISGLTGLRNYNEQKSSFCSKRTVCEAFDNLIKNANFKYIFLS
                     YNNEGLMSLSEIKEIMSYYGRYLFKTKDYKRFQADKSSNRNIKSYRTKEYLHCLVK"
     gene            3598..4245
                     /locus_tag="AC04VQ_00890"
     CDS             3598..4245
                     /locus_tag="AC04VQ_00890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRM11 family SAM-dependent methyltransferase"
                     /protein_id="HIO9552814.1"
                     /translation="MWIINKRDNSGVHNGNYHGNFVPQIPHQLLLRYTKKGDWVLDPF
                     MGSGTTLIEAQRLRRNSVGIELQESVAEEAKARINKEHLNGINSIVEVGDSRTFSYTN
                     ILKKANIEKFQFAIYHPPYGSIIKFSDSKDDLSNSRSLNDFLKKFGQVVDNSSNYLEK
                     NRYCALVIGDMYKNGQVIPLGFECMNIFLKRGFQLKAILVKNFGDTKAKHNKQGI"
     gene            complement(4735..5016)
                     /locus_tag="AC04VQ_00895"
     CDS             complement(4735..5016)
                     /locus_tag="AC04VQ_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552815.1"
                     /translation="MNKIKKINNNKKLKTQNVKLIYDTEKTIIKTCKHVCKYKDKNMQ
                     SLSNPIKERIIIKKKNKRAHEAHLQANFIQNCDSKPLSELLGLNNDLPL"
     gene            complement(5089..5541)
                     /locus_tag="AC04VQ_00900"
     CDS             complement(5089..5541)
                     /locus_tag="AC04VQ_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552816.1"
                     /translation="MRTNSFHVFIVNKDTFPVHLKYMFAGTHTNNNPDIFIANSKFEN
                     NKSTLSYKAELTVASMIADISRIRIGDKILFYLTSHDGKPGKFFGGFKAKSAAFYSKD
                     EDNYLKNELNKSLQFRVLIEADSVYPEGVTEQDALDNLEGINHPSEMC"
     gene            complement(5781..6104)
                     /locus_tag="AC04VQ_00905"
     CDS             complement(5781..6104)
                     /locus_tag="AC04VQ_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552817.1"
                     /translation="MQNNYYDKSSNFIYSIISLDDCLDEIEKRNVDKDYALHRWYNYH
                     TSIYCEEIFCDYGAIKEKDKFNHDVDIYIDGIPYDVKLTVYPAALSNRPYNLKKRKDR
                     NEMIK"
     gene            complement(6209..6694)
                     /locus_tag="AC04VQ_00910"
     CDS             complement(6209..6694)
                     /locus_tag="AC04VQ_00910"
                     /inference="COORDINATES: protein motif:HMM:NF017157.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552818.1"
                     /translation="MDEFKGKYKSLVFGNGADWARASSSLSKKYIIEFDKDITPGNRI
                     DRIRTIGFTKNSFSQQINKRIIKDIKKQRCVVLGTSNPEVDHKNGRKNDNRLNMVDKQ
                     KLDDFQPLSKAANDAKRQFCKDCKNTGIRFDAKQLGYPMSFYKGGKKHNNKTTGCIGC
                     Y"
     gene            complement(6771..6986)
                     /locus_tag="AC04VQ_00915"
     CDS             complement(6771..6986)
                     /locus_tag="AC04VQ_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIO9552819.1"
                     /translation="MNDLQNKLGKRIKELRLQSGLSQEKFALKIGMDRTYFASVELGK
                     RNISLQNLEKICKGFNVSLEELFKGIK"
     gene            7194..7376
                     /locus_tag="AC04VQ_00920"
     CDS             7194..7376
                     /locus_tag="AC04VQ_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552820.1"
                     /translation="MAFREALVSGARSKILPGVLKIQKILKNCKIMGESGGKCHIMWN
                     QQRTALVFGGVARDRK"
     gene            7580..7993
                     /locus_tag="AC04VQ_00925"
     CDS             7580..7993
                     /locus_tag="AC04VQ_00925"
                     /inference="COORDINATES: protein motif:HMM:NF014439.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIO9552821.1"
                     /translation="MLQFYGTYYHRIDDKGRVALPMKFRKTYDRALNNEEISSIDLVI
                     TPSPSKDGTEGRECLYVFTTEDFDVYVDSIFESAGGYNPRNKKHEQLMRALHAEVEDI
                     TIDSAKRINIPAKFRKIAGLGKDVAIVGNAGHFEI"
     gene            8060..9040
                     /gene="rsmH"
                     /locus_tag="AC04VQ_00930"
                     /pseudo
     CDS             8060..9040
                     /gene="rsmH"
                     /locus_tag="AC04VQ_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013919.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
     gene            9048..9830
                     /locus_tag="AC04VQ_00935"
     CDS             9048..9830
                     /locus_tag="AC04VQ_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552822.1"
                     /translation="MPTLRTNIQKGKFSNYGSSKFSTPTSNTKYFSERRVKQPTMRGS
                     FPRAVKWSLDDEGITNRRSDYNRSINLSQSASRPSRNFEFGAAQTIATANFSNGIYDG
                     NAARKLRPVSVPKTQKAAPKERNMHAVQGDITARSNMKTAAMSPMIVTLAKVAAVLVV
                     LVAVLAFIRVGMTSATVSNGLASQEISKAIDGELVSKNALEVQDSSLANSGRIRQVAS
                     QYSLVVPSAIQVFDIGQDVLAYDDNNHVSLVESLDRAAKSNP"
     gene            10000..11505
                     /locus_tag="AC04VQ_00940"
                     /pseudo
     CDS             10000..11505
                     /locus_tag="AC04VQ_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
     gene            11507..12199
                     /locus_tag="AC04VQ_00945"
                     /pseudo
     CDS             11507..12199
                     /locus_tag="AC04VQ_00945"
                     /inference="COORDINATES: protein motif:HMM:NF019850.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
     gene            12473..12838
                     /locus_tag="AC04VQ_00950"
     CDS             12473..12838
                     /locus_tag="AC04VQ_00950"
                     /inference="COORDINATES: protein motif:HMM:NF014874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIO9552823.1"
                     /translation="MMADIMPSKVIQVLNNLKGESGRFEKLTSKQGFSMINDAYNANP
                     QSMVGSLETLATTKCSGKRIAFLGDMLELGDYTQKAHEEVGLAVADNKIDILISVGKF
                     SKDIAKAAEKNGMRPENIF"
     gene            13106..13165
                     /locus_tag="AC04VQ_00955"
                     /pseudo
     CDS             13106..13165
                     /locus_tag="AC04VQ_00955"
                     /inference="COORDINATES: protein motif:HMM:NF022030.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13166..13441
                     /locus_tag="AC04VQ_00960"
     CDS             13166..13441
                     /locus_tag="AC04VQ_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552824.1"
                     /translation="MLIAMVLTITIMGIWSPEVIALVLAILAAGLLGLIDDVSKVVFH
                     RSLGLTPTVKLIFQFLIATVFILYAINSLGISPIVTIPFIIDLDLGI"
     gene            13475..13915
                     /locus_tag="AC04VQ_00965"
     CDS             13475..13915
                     /locus_tag="AC04VQ_00965"
                     /inference="COORDINATES: protein motif:HMM:NF013146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HIO9552825.1"
                     /translation="MPWLYLILIDILLVGMCNACNLADGLDGLAAGSSVIIMVVMAAV
                     SYRADSLSCAIISGALAGGCIGFLWHNAFPADIFMGDTGSLALGMGLACLSVLTKTEV
                     LSIIIGGMYVVEAFSVFIQVLHYKRTKKRIFLMAPLHHHFEKKG"
     gene            14027..14377
                     /locus_tag="AC04VQ_00970"
     CDS             14027..14377
                     /locus_tag="AC04VQ_00970"
                     /inference="COORDINATES: protein motif:HMM:NF045460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552826.1"
                     /translation="MGEVQLLKDKNYAPKNLGDVCVLGLGKTGNVVASYMCELLGKRV
                     KSVHVYAGEKKEFAMRAADRLLKCGATVSFDDKELKHKFDLCVTSPGISENDPLIKTA
                     KENCNEVISEVEFA"
     gene            14417..15445
                     /gene="murD"
                     /locus_tag="AC04VQ_00975"
                     /pseudo
     CDS             14417..15445
                     /gene="murD"
                     /locus_tag="AC04VQ_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
     gene            15435..15671
                     /locus_tag="AC04VQ_00980"
     CDS             15435..15671
                     /locus_tag="AC04VQ_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552827.1"
                     /translation="MSAKEKKEKLTPAKNVTADILVPRLIFILSVLALLLFGLVMVFS
                     ASNVEALNNNENPYSYLLKQIFFDAFGIVVAIAI"
     gene            15798..17000
                     /locus_tag="AC04VQ_00985"
                     /pseudo
     CDS             15798..17000
                     /locus_tag="AC04VQ_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
     gene            17002..17355
                     /locus_tag="AC04VQ_00990"
                     /pseudo
     CDS             17002..17355
                     /locus_tag="AC04VQ_00990"
                     /inference="COORDINATES: protein motif:HMM:NF015022.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - obsolete lipid glycosylation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
     gene            17380..>18073
                     /locus_tag="AC04VQ_00995"
     CDS             17380..>18073
                     /locus_tag="AC04VQ_00995"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - obsolete lipid glycosylation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HIO9552828.1"
                     /translation="MANKYLCKRANATCLTYDKAKIKSCNNVYITGNPVRASIFSAKK
                     TDGRKMLNIPENAKVLLVFGGSLGAAHINNALLKMLDELLERKDLHIILITGDKQYEE
                     VKSKVDLDAAKADRYHVLAYQNKMAETLAASDMCISRAGASTIAELTARKLPALLIPY
                     PHATENHQFYNAKALLDAGCVMYLNDEAVSSPEFKKAVVKLIDNDDMREDMHEAYSKF
                     DAENSAKKIADII"
     assembly_gap    18074..18173
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18319..19428
                     /gene="murC"
                     /locus_tag="AC04VQ_01000"
     CDS             18319..19428
                     /gene="murC"
                     /locus_tag="AC04VQ_01000"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIO9552829.1"
                     /translation="MGKTHINSVHFIGIGGIGMSGIAIVANAQGMKVSGSDLRSSNLT
                     DELKSQGIKIYIGNRSENIGTGDNKPDVVVVSTAIMEHNPELKAAKEAKIPIWHRAEL
                     LAYLGRNKKTLAVTGTHGKTSTSSMLASALEQIGDDPSFLIGGVVKPYNTNAKSGEGE
                     HYVVEADESDKSFTYLSPYAAIITNIEADHLDHYKDMDEIYEKFETFIKLVPDNGLAV
                     CSGDDKNLTEITKRSAKNVITYGFGEDCDCILSNYKTKGTGCEFSLTLPVFNVGNSNR
                     TEEAKFDCSLPYNPGIHYAENAASVVVLLDALGFDASQVVDALKNFGGIKRRFDVLGC
                     EGGVTVVDDYAHHPTEIAATVKAAFELDFKNVHVV"
     gene            19546..19761
                     /locus_tag="AC04VQ_01005"
     CDS             19546..19761
                     /locus_tag="AC04VQ_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552830.1"
                     /translation="MPVQGVTGYTFLEIVKNDAGKNSPKTYYVPHMLDIIDHISAIVD
                     NGDLVITMGAGDITSVGPQILEAIKNK"
     gene            19775..20203
                     /locus_tag="AC04VQ_01010"
     CDS             19775..20203
                     /locus_tag="AC04VQ_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIO9552831.1"
                     /translation="MEELKIEGYNARIKKDEPMSNHTTYHIGGLAKYFVYVNDAQALK
                     ETIEYGKERGIKYTIVGDCSNFLVSDDGFDGAIIKLAGDFRKFSFDLNRNICTVGAGV
                     KTAKIYNEFQKNYYKGLGYCVGIPGTIGGAVKMNAGRKRQ"
     gene            20324..20704
                     /locus_tag="AC04VQ_01015"
     CDS             20324..20704
                     /locus_tag="AC04VQ_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014872.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552832.1"
                     /translation="MQVNMKFKPTKSENEREYMRNRAKLMTMERRSHQPIGSLSCGSV
                     FKNPPDNFAGKLIEEAGFKNAKVGGAQVSDVHSNFIVNTGNAKAADVVELIRQIQKKV
                     YDQNKIKLIPEVQFLGFKEPVSLF"
     gene            20715..21065
                     /locus_tag="AC04VQ_01020"
     CDS             20715..21065
                     /locus_tag="AC04VQ_01020"
                     /inference="COORDINATES: protein motif:HMM:NF020067.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIO9552833.1"
                     /translation="MRKKNKVDMSDVEFDTKNPTLVKSEKAKRKEDRKKRKRFRVIRN
                     VILVIVAVLLVSLIGSYIYLYSTDAFPIENVQVNGVEHLTDDEMSQLMDIPEDTTLLK
                     VDTEIIKKRLKRDA"
     gene            21087..21212
                     /locus_tag="AC04VQ_01025"
     CDS             21087..21212
                     /locus_tag="AC04VQ_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552834.1"
                     /translation="MSFPNTLVINVTERSVTAIVEVPVSSSDKTMRNWAISSDHM"
     gene            21216..21605
                     /locus_tag="AC04VQ_01030"
     CDS             21216..21605
                     /locus_tag="AC04VQ_01030"
                     /inference="COORDINATES: protein motif:HMM:NF015739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIO9552835.1"
                     /translation="MPVPDKNSEAAKNISQQIFADVEGVMHIVDVSPSVQPEIGSYCS
                     DDPVNNAIDVVAAMTTDLKNQVKYVKASDANSTNLFLNNGVEIAVGTSENLREKERVA
                     EELLSKYEGKISYINVRNASNPTWRSI"
     gene            21605..>22335
                     /gene="hypE"
                     /locus_tag="AC04VQ_01035"
     CDS             21605..>22335
                     /gene="hypE"
                     /locus_tag="AC04VQ_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HIO9552836.1"
                     /translation="MANQDKILMSHGSGGSMMSDLIEAEFVNAYIDKDLYLGDDATVL
                     DKMKLGDGEKIAMSTDSYVVHPLTFPGADIGRLAVCGTVNDISTSGAKPVALSIGFIL
                     EEGLEISTLKDICHSIAKSATEAGVKIVTGDTKVVEHGKCDGIYINTSGIGIVPNGIN
                     LSGRNIKPGDVVLISGTIGDHGITIMSCREGLNFSTNLKSDAAPLNSLVADVLKAAPD
                     TRCFRDPTRGGVASALNEFAAQSNV"
BASE COUNT         6862 a         3859 c         4451 g         7063 t
ORIGIN      
        1 acatttcgcc cccttaaatt atgcgagtat tatacagtat ttattggatt tcccgcaata
       61 aaagacttga tgttattttc tgtgatttcc ataatgcgct ggcggcattc aattgacgtc
      121 catgaaatgt gcggtgtgat tatgcaattt ttcgcagcca gaagtggatt gtcagccttg
      181 attggctcgg tcgagacaac gtccacagcg gcgcctccaa cttttccaga atttagtgca
      241 ctggcgaggt ccgcttcgtt gattaggcca cctcgaccat tgttaacaat taagacgccg
      301 tttttcatct tcgcgatgtt ttcggcgttg atgatttcct tagtttcgtc gtttaaaatg
      361 cagtgcagca caatcacgtc agatttgccg aagagctcat ccaagctcac aaaatcgacg
      421 aagccacgtc agtcatcctt tggatgaggc gtgtagacaa caacattcat gcccatcgcc
      481 tttgcggtct tcgcagtttg ctggccgata gacccaagac caatgattcc aattgtttta
      541 tcagtgagct caataacggg cttcttccaa aagcagaagt ctcgcgaatt ttgccattct
      601 ccattatgca ccgaagcatt gtgaagcccg acttgatttg tcatctcaag cattaaagca
      661 attgcatatt gcgaagtaca attcgtgctg taagctggca cgttagaaac agcgattcct
      721 tttgctctcg cataatccac atccacaata ttgtagccag tagcaagtac gctaacatat
      781 ttaagatttg gacatgcatc aattacttct tttgtgattg gagctttgtt cgtgattgca
      841 atttccgcgt caccgatttt ttcaacaacc gcatccgcgc cgccatcaac gcgttcgata
      901 atttcaaatt cgccataatt cgcaagccaa tcccaactaa gatcgcccgg gttctcagta
      961 tatgcatcca aaactacaat cttcataccc ctattttacc atttccccgg ggcccacact
     1021 cgatttactc gtggcattta ctcgatttcc ccggggccca cactcgattg tctccgtcca
     1081 tttactcgat ttttatttct ctttatagta aaattaaatc gttaaagggg aagagatgat
     1141 taagaaaagt agagtagaag cttttagtga tggagtgctt gcgattgttg ccacaattat
     1201 ggtgcttgag ctggtgcttc ctggaggtgt tgcctggggc gatttcgccg cgcagtggcc
     1261 ggtgctcctt gccttcgtaa ttagtttctt tcaaatttat attaaccttt ataatcacaa
     1321 caagcttttc ggcaaaatcg aatgcgtcgg ccgtcatgtt tatcttttaa acggcttgtg
     1381 gcttctcttc gcgtgtttcg tgcctttcac cgttcactac ctcggccaca atcctgatgc
     1441 cacgctcccg atgctaattt acctagttaa tcttttgctt tgggccatca gttttcaaat
     1501 tcttgatatc actattctcc acgaaaaccc caaagcggcg aaggacgaat ccacaaccat
     1561 catcacgcga atcatctact acgtaggcct tttcatcgca ggcatcacag caatcttcat
     1621 tccacgcatc agccttttca tcatcctcat cgtcgtggca ggcgaaatca ttacaacttt
     1681 cttcaaagcc accaaaactc acgatgaagc ttgcaagtaa ttttttgatt atcttataaa
     1741 gttagtgtag gtcttctttt ccaattcagg aaggccgaat ttttctttgc ctaattgaat
     1801 actgcgcatt aaaaatgtca tattgtttgc aagagtgcgc atggtttgca agccttcggc
     1861 atcctgcttc gcctcttcgg cattgtttcc gtgaattcca tttcaatatt ggctactagc
     1921 aacaggcatc ccagcaatcg taaagtattt attgagttga tcaaagctcg aagtcaagcc
     1981 tccgcgacgg gccgaaacca cacaagctcc aactttcatt cgaacatctt tgcgcagcat
     2041 tacatagaat agtctgtcca taaatgcatt aagagttgca ttcgacgaag cgaagtaaac
     2101 tggtgcacca acaacaagcc cgtcagcttc ttccaccttc ggcaaaatct catttacgat
     2161 atcatcaaac ttgcacttgc cagccttata gcaataaccg caagccttac atccaggaat
     2221 atccaaatgc ccaacttgaa caatctcggc ctcaacgcct tccgcatcaa aaaccttctt
     2281 catctcacta agagcagtaa aagtactacc atcctcatgc ggacttccat taatcatcaa
     2341 aactttcata cccacccctt taactgaatc tattatataa cataattttt ttaaataatt
     2401 ttaagattta cgataatatt tcggaattat tactattctg tatgttaaga taagaaaaaa
     2461 agatggcgta tttgttaata attctgacga atacaaattc ccaggtcatc cttgtaaaat
     2521 ttaattattt atgaattata ttggatcgaa atattcttta attgactttt tagttgaatc
     2581 aatagatgat gtcactggat tcaattcagg tgatggccga acttttgctg atttatttgc
     2641 tggtacgggt atcgttggag ctacttttaa agagcgaggg tataaagtca tttctaatga
     2701 tattcaatat tatagttatg taataaataa acacttaatt gaaaattgta aagaatatgg
     2761 caattcaaaa attaatgatt taaataatat taaatttaaa gaaggtttta tatataaaaa
     2821 ttattgttca ggctcaggct ctggacgaaa ttattttaca gatgataatg gaaaaaaatg
     2881 tgacgctata agagaagaaa ttgataatct aaagacaaat aaaaagatta gcaatggcga
     2941 atattattat tgtcttgcta gtttgattga ttctgttgat aaatgcgcaa atactgcatc
     3001 tgtttacggt gcatttttaa aacacataaa agaatcagca aagaaggatt tagaattacg
     3061 acctttacca actataaacg ggccaaaagg aaaagtttat aacgaagata ttaatatttt
     3121 gattaaaagg attaatggag atattttata cttggatcct ccttataata gtcgacagta
     3181 ttgctcgaat tatcatttac tagagacaat tgcaaaatat gacaatcctg aaattagcgg
     3241 tttaactgga ctaagaaatt ataatgagca aaaaagttct ttttgttcaa aaagaactgt
     3301 ttgtgaggct tttgataatt taattaaaaa tgctaatttt aaatacatat ttttgagcta
     3361 taacaatgag ggtcttatgt ctctaagcga aattaaagaa attatgagtt attacggacg
     3421 atatttgttt aaaactaaag actataaaag gtttcaggca gataaaagtt ctaatagaaa
     3481 tattaaatct tataggacaa aagaatattt gcactgttta gtaaagtaaa tatgccaaaa
     3541 tttaatgaca tagatttaaa aaagtgaaaa gagtacgaga gtatttcaac tgattcattg
     3601 tggataatta acaaaagaga taactctggt gttcataatg gaaattatca tggtaatttt
     3661 gttccgcaaa ttcctcatca attattattg cgatatacaa aaaaaggaga ttgggtgtta
     3721 gatcctttta tgggtagtgg aactacttta attgaagcac aaagattgcg gagaaattct
     3781 gttggtattg aacttcaaga atccgttgca gaagaagcta aagcaagaat aaataaggag
     3841 catttaaatg gcataaactc cattgttgag gtaggagata gcagaacgtt ctcgtataca
     3901 aatattctta aaaaagcaaa tatagaaaag tttcaatttg caatatatca tcctccttac
     3961 ggaagcatta ttaagttttc tgattctaag gatgatctct cgaattcaag aagccttaat
     4021 gatttcttaa aaaaatttgg acaagttgtt gacaactcct caaattattt agaaaaaaat
     4081 cgatattgcg ctttagttat tggtgatatg tacaaaaacg gacaagttat tcctttagga
     4141 tttgaatgta tgaatatatt tttaaaaaga ggatttcaat taaaggccat tttagtcaag
     4201 aattttggcg atacaaaagc taaacataat aaacaaggaa tatgacgtta tagagcccta
     4261 gcaagtgatt tttacatatt taaacacgaa tacattttta tttttaagaa attattttaa
     4321 agaatatatt acgttgtcaa tattctcata atgaatagta ttgttaaaaa tagagtaatt
     4381 tatgtttgca actttattaa tagaaacatt cttactttta ctaattttta atttgtaatt
     4441 gttacattca gaaatatatt cttttaagtt ttttgattta ttaagcttcc ctttatcgtt
     4501 gttaaaatca gctactacaa taggattaat cacaattgct ttatttttgt aaattggggc
     4561 aatgtagtga ttaagtcaaa gtaaatattt ttgtaactga tagcttatga tacctttata
     4621 aggttttgtg catttaagtt caataatata tatatagaaa catttttttg tttcttgcgt
     4681 tagcattacg tcgatacttt gcattccaac tccgcaacta acttcatttc caattcataa
     4741 tggaaggtca ttatttaggc ctaataattc agacaaaggt ttagaatcac aattttgaat
     4801 aaaattggct tgtaaatgag cttcgtgtgc tcttttattt tttttcttta ttattattct
     4861 ttctttaata ggattactta gagattgcat attcttatct ttgtatttac agacgtgttt
     4921 acacgttttg attattgttt tttccgtatc atatattaat ttaacatttt gcgtctttaa
     4981 ttttttatta ttatttattt ttttaatttt attcataata gaattgtatt caaattcatt
     5041 aattgcagta caacctctat tgcccttgag ttttctataa attagacttc agcacatttc
     5101 gcttggatga tttatacctt ctaaattatc aagagcatct tgttctgtta ctccttctgg
     5161 gtaaactgag tccgcttcaa tgagcactct gaactgtaag gatttattta attcattttt
     5221 taaatagtta tcttcatctt ttgagtaaaa tgctgcactt tttgctttga agccgccgaa
     5281 aaatttccct ggttttccat cgtgagaagt taggtagaac aaaattttat cacctatcct
     5341 aattcttgat atatctgcta tcattgatgc aaccgtaagc tctgctttgt aacttaatgt
     5401 gcttttattg ttttcgaatt tactatttgc gatgaatata tcaggattat tattagtatg
     5461 agttcccgca aacatatact ttaagtgaac agggaacgta tccttattta ctataaatac
     5521 gtgaaatgaa ttagttctca ttattctcct tccaaatata tgagttcaga ataaagttcg
     5581 tatttgttgt cattgtcagt aatgacaatt ttgttttttc cgtacttttt ttgaaaatcc
     5641 ataaaacttt taatatggct tctaataaga tcgaaatcac ttttcatttt taaagattct
     5701 tgctggtttt ttccatcaca aactacatat atgcgattta ataattgttt tctagattgt
     5761 tgagattgat gctcgtagta tcatttgatc atctcgtttc gatctttacg tttttttaga
     5821 ttatatggtc tatttgataa cgcagcaggg taaacagtaa gttttacatc ataaggaatt
     5881 ccatctatat aaatatctac atcatgatta aatttatctt tttcttttat agcaccgtaa
     5941 tcacagaata tttcttcaca atatattgaa gtgtgatagt tataccacct atgaagagca
     6001 taatccttgt caacatttct tttttcgatt tcatcaaggc agtcatctag agaaataatg
     6061 ctataaataa agttagacga tttatcataa taattatttt gcatttttca attagcctct
     6121 atcagctgaa gagtttcttt aagatctttt tcaagttggg gattcattat ttcttcatta
     6181 agtgtttttt aaattcaata ggatcatatc aataacaacc aatgcatcca gtagttttat
     6241 tgttatgttt cttaccgccc ttatagaaag acattggata acctaattgt tttgcatcga
     6301 aacgaattcc tgtattttta caatctttac agaattgtct tttggcgtca ttagctgctt
     6361 tacttaatgg ctgaaagtca tcaagttttt gcttatcaac catatttaaa cgattatcgt
     6421 tcttccttcc atttttatga tcaacttcag ggtttgaagt acctaataca acgcatcgtt
     6481 gtttctttat gtctttaatt attctcttgt tgatctgttg agaaaaacta ttttttgtaa
     6541 aacctattgt gcgaattctg tctattctat ttccaggtgt aatgtcttta tcaaattcaa
     6601 ttatatattt tttggatagt gatgaactag ctctagccca atcagcacca tttccaaaaa
     6661 ccaaactttt atatttgcct ttaaattcat caatacttac tcatcttgac ttaccttttt
     6721 tattaggttt tgctaactct aaaaacaaat ctttcttcga tttagacatt ttattttatt
     6781 cctttaaata attcttcaag agagacgtta aatcctttac aaattttttc taaattttgc
     6841 aatgatatgt ttcttttgcc cagctctacg gatgcaaagt atgttctatc cattcctatt
     6901 tttaaggcaa acttctcttg actaaggccg ctttgtagcc ttagttcttt aattcgcttt
     6961 ccaagcttat tctgcaaatc attcataggc cctatattat taaaatttgt ttatgagtca
     7021 acggactata agcaacatta ttatcattca aacattattt actattttac tgtaataaat
     7081 aattatttat ttttatttat ctttgaattg tcggttcata agtgaagaat tttggaatgt
     7141 gtgacccgaa cgattctgga gcgccatgcg cttgctggcg gcttgcccgc actttggcat
     7201 tccgcgaagc cctagtgagc ggcgcacgtt ccaaaattct tcctggtgta ttaaaaatcc
     7261 aaaaaatatt aaaaaattgt aaaataatgg gtgaaagtgg gggaaaatgt catataatgt
     7321 ggaatcaaca aagaacggca ttggtcttcg gtggcgttgc cagggaccgc aaatgaacta
     7381 aaaatgcagt tcagacgaac aaaaaaatgt agctgccgtt tatatttgtt aaacgcacga
     7441 gcagacggaa atgttgttac atgtctgcaa tttgcgtaag tacgcggcga atgtgcaaac
     7501 gtagtacaaa aatttttaaa aacaaaacac aaacgcacac cgcacattcg catccgagaa
     7561 gggaaggaaa aagaaaaaga tgcttcagtt ttatggtaca tattaccatc gcatcgatga
     7621 caagggtcgt gtggcgcttc cgatgaagtt ccgcaagact tatgatcgtg cgctgaacaa
     7681 tgaggaaatc tcaagcatcg atttggtaat cacgccttcc ccttcgaaag acggaaccga
     7741 aggtcgtgaa tgcttgtatg tttttacaac cgaggatttt gacgtttacg ttgattcgat
     7801 tttcgaaagc gcaggcggtt acaaccctcg caacaaaaaa catgagcagt tgatgcgtgc
     7861 ccttcatgcc gaagttgagg acatcacgat tgattcggca aagcgtatta acattccagc
     7921 gaagtttaga aaaattgctg gacttggtaa agatgtggcc attgttggaa acgcgggcca
     7981 cttcgaaatt tgagacgcta agcgtatgga aagtgaactt gagtcgattg acttggagac
     8041 cttgatatac gaatagcata tgagcacttt gaaaaacgaa tatcggcaca ttcctgtttt
     8101 actggatgaa attgttgcgc aggctaaggg ttttgaagga gacatttttg ttgactgcac
     8161 cttgggaggc gctggtcatt cggttgagat tgcaaaggtg attggcaagg acggtctttt
     8221 gattggaatt gatcaagaca ccgaggcgat cgaagcagcg gatactcggc ttcggagttt
     8281 gccggaggaa tcaaggcctg aaattaggct tgtgaatgat aactttggca acctcgacga
     8341 gataatgttg cagcaggaaa ttcctggttt tggtctcatt ttgatggacc tgggtatgtc
     8401 cagccatcaa attgatgata tctctagagg cttctccttt aaggatgaca gcccgcttga
     8461 catgcgaatg gacccggcta aacaaacttt aaatgcagct aagatcgtta atttctatag
     8521 catgggcgat cttattcggg tccttcgcga tctcggcgaa gagaaatgag cgaaggaaat
     8581 tgcacgcgag attgtgaagg ctcgcgaaaa aacggagatt aaaagtagct atcagcttgt
     8641 tgacattatt aaacgtgcaa ttccagctgc cgcaagacga caaggaggcc accccgcaaa
     8701 gcgaactttt caagcgcttc gaattgaagt caacgatgag ttgaaggttt tgaaaagtgg
     8761 acttgatgca gctattaggt ggcttaatcc aggtggaaaa atcatggtcc ttacgtatca
     8821 ttcgcttgaa gacaggattg ttaaggacag tttttcagaa atggttaaca gatgcacatg
     8881 ccctccagat ctcgcgacat gcatttgtgg caaacagccg gtattaaaag ttttgacaag
     8941 taaaccgata attccaagtg aggaggaaat cgaaaacaat tcgcgaagcc gaagcgcaaa
     9001 gcttcgagtt gctcaaaaat taagtgaaac cgtagattaa atgaactatg ccaacactta
     9061 gaactaacat acaaaaagga aaatttagca actatggctc ttcgaagttt tcgactccaa
     9121 cttctaatac taaatatttt tcagaacgtc gcgtaaagca acctacaatg cgaggctctt
     9181 ttcctcgtgc ggtcaagtgg tctttggatg acgaaggtat tacaaaccgc cgaagcgatt
     9241 acaaccgttc tattaactta agtcaaagtg cctcgcgtcc aagtagaaat tttgaatttg
     9301 gtgcagcaca aactattgca acagcaaatt tttcaaatgg aatttatgat ggaaatgcgg
     9361 cgcgtaagct tcgtcctgtt tctgttccta aaactcagaa ggcagctcct aaagagcgca
     9421 acatgcatgc agttcaaggg gacattacag cacgaagcaa tatgaaaacc gctgctatgt
     9481 cgcctatgat tgtgacgctt gcaaaagttg ctgctgtact ggtagttttg gttgctgttt
     9541 tagcattcat tcgtgttggc atgacttcgg caacagtttc aaacggactt gcttcacaag
     9601 aaatttcaaa agcaatcgat ggtgaacttg tttcgaagaa tgctttggaa gttcaagatt
     9661 cgtctcttgc taattccgga cgcatccgtc aagttgcttc gcaatattca ttggtggttc
     9721 caagcgccat tcaagttttt gatatcggcc aagatgtgtt ggcctatgat gataacaatc
     9781 atgtttcttt agttgaaagt ttagaccggg cagcaaaatc taacccataa gcgaggccct
     9841 atgtctttca atgtttcgcc gaaaagaatt tatgcagccc ttgcagtttt tggtgttctt
     9901 gttctaatta tactgctcag gctgttttat ttgcaagtta ttgatgccga agcaaaaaca
     9961 aaagaggcaa tcgcaactcg aacaattcgt tatgacacaa tgccaaaacg tggtgccatt
    10021 tatgatcgaa acggaaatgt gcttgcatat agtaaagaag ccaaaacaat ttatgccaat
    10081 ccttctgaga ttgaaaatga cgaagaggta gcaaccaagc ttgcaaatgt tttgggcgga
    10141 ggcgcaagcg attacatcga ttctataaat aacaaaaagt tgaaattttc atatgttaag
    10201 cgtcgtgtgg atgttgaggt tgctcaacaa ctcaaagatg aaaatattaa gggcatttat
    10261 tttcaagaag accagaagcg agtttatcca tatgggcagg tcgcagggca gattattgga
    10321 gctattaaca tagatggtga tggcctttgc ggagttgaac tttactatga tgatattttg
    10381 cgaggctcca gcggtcgaac tgttcgtcaa caaggcaacg aaggtatgcc aatccctggc
    10441 ggagttttgc aagagactca agttattgat ggccaagaca taatgctttc tattgatgtt
    10501 gagcttcaac aaaaagttga gaacactctt gttgaatgga ataaaaaact tggaagtaaa
    10561 agtatgcatt ccattttaat ggatccaact aatggtgaaa tttatgcggc ggcttcgctt
    10621 cctttgttaa atccggctga cttaagttct gccgaagaag gggcaaccga tcttaaatgc
    10681 attagcacaa cttatgaacc aggctctacc ttcaaaactt tggctgcaac agctatttta
    10741 gaaaacaatg caatgacacc tgacactgag gtattttgtc cgaacagtat tacagctgac
    10801 gaatttgttg ttaaggatgc tcatgacaga gccgatcaaa cttttacttt ccgtcagatt
    10861 atagaaaagt catctaatgt tggaatttca ctttcaactg aacaactcgg ttgagataaa
    10921 tatcatgaca aacttttgca atataaaatt aacgaagaca caggcgtcga ctatccggga
    10981 gacactggag ggtatttgct tgacttcgat aagtggtcaa aaattcatgc ctataatatt
    11041 tcatttgggc aaggtgtcac tgtaacacca cttcagatgt gtcgttttta ttcatcgcta
    11101 gctaatggag gttatgcaaa aactcctcat ttcttgatga gatatcttca agctgaacgt
    11161 gatgaagaat atggcgaaga agaattgatc tataacaaaa atgcaattcc ggtaatggtt
    11221 gatattttaa aaggagttct tacaaacgga actggaactc acgcaaatat tccgggatat
    11281 ggaattgcgg gtaaaactgg aaccggagaa atttcagatt cgaatggtaa atatttaaca
    11341 ggaacctatt atgcttcgtt tgtaggattt ttggccgaag caaatgttcc aatgttatgt
    11401 tttgtcggag gcgaacgagt tccaagcgaa gccgccgtta cgcctgtttg gaaggacatt
    11461 atgtcttata caattgagag actaaagatt gcatcgaagg gctaatatga atttaaatgc
    11521 tgaacaaata cgcgaagctt gtgatggaga atttttagtt cctccgcttg atactactgt
    11581 tttttcaaca gcactaaaaa ttgattcacg aaaaattgat tgcggagatt tgtttgttgc
    11641 tcttaagggt gaacacagcg atggctttaa atatataaat gacgcaatta atgatcgcgc
    11701 aagtattgtt gtttgtgaca gagatattgc cgaagacaca aaaattattg ctaatgatca
    11761 tgctgcttca attattaagg ctaaagatgc acgtcaggca attcgtgaca ttactattga
    11821 gtggcgcaaa aatattatgg gaaaaattat tggtataact ggttctgttg gaaagacaag
    11881 taccaagaat tttgtttcgc aagttttaag ttctaaatat caagtaactt caactgacgg
    11941 aaattacaat aatgatctcg ggcttccaat tactctttgc aaagcaaacc ttgaagatga
    12001 ttttgtagtt gcagaaatgg gcatgaatgc tcctggcgaa attgcaaacc tttgtgatat
    12061 tgctcaacca gaatgaggaa tcataacaaa tgttggcact gcgcatattg aatacttagg
    12121 gtcacgagaa aatatagcaa aagcaaaagc cgagcttgcg caggcaattc cagacaaaat
    12181 tggaaagatg tttttgtgaa cggatgatga tttccgcgac tttataattg attatgcaaa
    12241 actaaaggag cgaaacattg aaacaatttt cttcggtgga actaaatata ttgaaagttt
    12301 agattctcca caagtttggt ttgagaattt agaaactaac gaagacggaa attccacttt
    12361 tgatgttttc ttaaaaggat ttattaattc cgatgctgat tcgttttcag ttaagttaaa
    12421 ccttcctgga aaatttaatg tcattaatgc gtgtgccgct tttgcagttg gaatgatggc
    12481 ggatatcatg ccttcgaaag ttattcaagt tttaaataat ttaaaaggtg aatctggaag
    12541 gtttgaaaaa cttacgtcta agcagggttt ttcaatgatt aatgatgctt ataatgcaaa
    12601 tcctcaaagc atggttgggt cgctagagac tctggcaact actaaatgta gcggaaagcg
    12661 aattgcattt ttaggagaca tgcttgaact tggagactac actcagaaag ctcacgaaga
    12721 agttggtctt gctgttgctg ataacaaaat cgatatttta attagcgttg gaaagttttc
    12781 aaaagacatt gcaaaggcag cagagaaaaa tggaatgagg cctgaaaata tattttgatt
    12841 tgaaaaaact gaagaagcaa aactcaagtt gcttgaagta acttgcaatg atgacgttgt
    12901 tttatttaaa gcatcaaata ttatgggttt tcaaaatgta gtccaggaac tggtggttta
    12961 gggaggaatt atgttacaag gttatgctca atatccatcc tttatagttt tttgttcaat
    13021 agctatgtgt gtattgttca gtttggtaat cacacctttt tgaaaacgca tcttaaaaaa
    13081 gagtcatatt ggtcaacaag ttagaatcga tggtccagaa actcattatg taaaacaagg
    13141 caccccaaca atgggtggag ttgtaatgtt aattgctatg gtcttgacaa ttacaattat
    13201 gggtatttgg tctcccgaag taattgcttt agttcttgca atattggcag caggattgct
    13261 tggtttgatt gacgatgttt cgaaagttgt ttttcatagg tccttgggtc ttaccccaac
    13321 cgttaaatta atatttcaat ttttaattgc aactgttttt attttatacg ccattaactc
    13381 tttgggtatt tctcctatag ttacaatccc atttattatt gatttagatt taggaatttg
    13441 aacaacagtt cttcctatag gtaacggaat tataatccct tggctttatt taattttgat
    13501 tgatattttg cttgtaggaa tgtgtaatgc ttgtaattta gctgacggcc ttgatggctt
    13561 agcagcaggt agtagcgtaa ttattatggt tgtaatggca gctgtttctt atcgtgcaga
    13621 ttcattgagc tgcgcaatta tttctggagc attggcaggc ggatgcatcg gatttttgtg
    13681 gcataacgca tttccggctg acatctttat gggcgataca ggttcgcttg ccttaggtat
    13741 ggggcttgca tgtctttcgg tcctaacaaa aacagaagtg ctttcaataa ttattggtgg
    13801 catgtatgtt gttgaagcgt tttcagtttt cattcaagtc cttcattaca aaagaacgaa
    13861 gaaacgaata tttttgatgg cgccacttca tcatcatttt gaaaagaagg gttgaagcga
    13921 gacaaaagtt gttgttcgct tttgaatcat aactggaatc ttatcttcgt ttggttttgc
    13981 aatttacttt tcttggacat tgcttattaa aatgggagca gtgtaaatgg gagaagttca
    14041 gttgttaaaa gataaaaatt acgcacccaa aaatttagga gatgtttgtg tacttggcct
    14101 aggaaagact ggtaatgttg ttgccagtta catgtgtgag cttttaggca aacgagttaa
    14161 gtcagtgcat gtttatgcag gtgagaaaaa agaatttgct atgcgtgcgg ctgatagatt
    14221 gttgaagtgc ggtgctactg tttcttttga tgataaagaa cttaagcata aatttgattt
    14281 gtgtgttaca agtccgggta tttctgaaaa tgatccttta attaaaactg caaaggaaaa
    14341 ttgcaacgaa gtaatcagcg aagttgagtt tgcctgacgt gaaagtgacg cacaggatac
    14401 atgagttgct gtaagcggaa ctaacggaaa gacaacaaca acttctttaa ttacacatat
    14461 tttgcagagc agtggaaagc atgccgaaag tattggaaat attggtaatg ttgcactgga
    14521 ttgtgtggct tcgaatttaa gaaagtcaaa atacgaacac acagatattt atgtttgcga
    14581 agtttcttcg tatcatttag cgctgtgtga taaatttgcg ccagatattt ctgttctttt
    14641 aaatattact cctgaccacc tagattggca tggaagtttt gagaattaca caaaagcaaa
    14701 acttgatatt tttaagaatg caaaaattgg cgttataaat attgatgata aagttatttc
    14761 agaaaacatt aacaagattg aaaaatttga tttagaacat ttgataaaaa atacgcacga
    14821 tgatgaaaat attattgtgg aatataaaaa tcaaactcat aacataattc caatttcaaa
    14881 tttaaaaatt attggcgaac ataattgtgt gaattctcga gcggctgcaa gtgcttgtgt
    14941 tgttttaggc ttagaagatt ctcaaattgc tgaaggcttg gcttcgttta cttcgttaga
    15001 acacagactt gaaaattgcg gaattgttgc gggagttcgt ttgttcaatg attcaaaagc
    15061 caccaacgta gattcagttt tggttgctat ggattctttt gaacgcggta aagctatatt
    15121 tttgctaggt ggaagagata aaaatacaaa tttagatgag cttatttcca aggcaaaaga
    15181 ccagcttaaa ggagttgttt gttatggcga agccaaagat cgcttcgttg aagcattcaa
    15241 aagtaaaatt aaagatgaaa atttcttaat tgagactgct gaaaacatgg gtggtgcttt
    15301 taagattgca atggagaaag cttttcccgg agattttgta gtgctgtctc ccgcatgtgc
    15361 ttcgtttgat gaatttgaaa gcttcgaaga acgcggaaaa gtttttaaat ctttagtgga
    15421 gaagtgtaaa gaaaatgtcg gctaaagaga aaaaagaaaa attaactcct gcgaaaaacg
    15481 ttactgccga cattttagtt cctcgcttaa tttttattct ttcagtctta gctttgttat
    15541 tgtttggttt ggttatggtt ttttcagcat ccaatgttga agctttaaat aacaacgaaa
    15601 atccctacag ctatttactt aagcaaattt tctttgatgc ttttggaatt gttgttgcca
    15661 tcgcaatttg aagatgaatt ccattttcta tttgaaaagg aaaatttttg tgagcttgtt
    15721 ttggactttt aatgtgcttt atgctggcaa ctttaattgg cggagctgat attttaggcg
    15781 cacgccgatg aatttatatt gggcctatta gttttcagcc atccgaattt gcaaaaataa
    15841 ttttggtttt aattgttgca aagtattttg ctgaatataa atatggtcat atagattttt
    15901 tgaaatgcgt tggaattgcc ggcggcgtta gtttatttat gtttgccttc attttgtttg
    15961 gtcagtcgga tcttggcaca acaataattt gttttgtagg aattttagca ttgcttgttt
    16021 ttgccggaat gccaatgata attttttgaa tccttttggg tgttgctata ttctttgggt
    16081 tatttgttgt tgttgcgtct ccttgaaggc ttgcgagatt gatggtattt ttggatcctt
    16141 ataatgatgg agccgaaggc tacggcgatg gttaccaatt aattagatct tattatgctt
    16201 tcgctgaagg tggattcttt ggagttggac ttggaaattc ttatgaaaaa tatcaatatc
    16261 tccccgaagc agagaccgac ttcatctttg caattattgg cgaagaactg ggcttttgtg
    16321 gatgcttttt agttattgtt ctttttagtg cgttaataat ttcaggattg ttaattgctg
    16381 caagatcaaa ttctcgcttt gctgctgcaa tatctggttc gtttgttatt atgattggct
    16441 ttcaagcctt cttaaatatt atgtgtgtaa taggatttgc cccaacaacc ggaaagccgc
    16501 ttccattcat ttcggccgga ggttcttctg taatagcaac tttaatgatg gttggatttg
    16561 tattggccgg gtccgaacaa gattcgcttc cagataaata tgaaaaacgc cgccaaggtt
    16621 ttaaggtcag cgctccaagt ggaggaggta ggattaaggc aacgtctgca gatgaagttc
    16681 ttgaaaaagt tacgaagcca cgacccacgc gttcaagtca atcatctact tcaagaacaa
    16741 agacaagcca gaagagaact tcggcgactc gtggtgttaa gaaagctcca aatattaaag
    16801 agccaaattt tatagcgaaa aatgacagaa agcgtgaaag caatcataaa agttatgctt
    16861 acaagaggtc gaaggctgcg ccaagtttta agccaagaac aacttcggca ataaaatcgg
    16921 ctccgaagtc aaatagcaga aatgctacaa aacctaagag cacaaaatca aagagcacaa
    16981 gcagaagcag gagaaagtaa tatgaaattt gtaattacat ccggaggaac tgcgggacac
    17041 attaatccat cgttggcttt ggctgaagaa ttagttgcgc gcaaacacga ggtggttttt
    17101 gccggcactc caaacagact tgagtcgaag ttagtgccgg atgctggtta tgaatttaag
    17161 gcttttgaag caaaaggttt caacagaaat aaaccatgaa caattttttc tgcgcttcgc
    17221 tcaattaata aaagttctaa acttgcaaag acttgacttg cgaagcaaaa acctgatgct
    17281 gttatagggt tcggctcata tgtttcaatc ccaatttgta gggcagcgtc aaaacttaat
    17341 attccattcg ccattcatga gcaaaattct gtcatgggaa tggcaaataa atatttatgc
    17401 aagcgcgcaa acgcaacatg tcttacttat gacaaagcaa aaattaaatc ttgcaacaat
    17461 gtttatatta cgggaaaccc tgttcgagca agcattttta gcgcgaaaaa aactgacgga
    17521 cgcaagatgt taaacattcc agaaaacgcg aaagtcttgc ttgtcttcgg aggtagtttg
    17581 ggagcggcac atattaataa tgcgttgcta aaaatgttag atgaacttct tgagcgtaaa
    17641 gatttacaca ttattctcat tactggcgat aaacaatacg aagaagtgaa atccaaagtt
    17701 gatttggatg ccgcgaaggc tgacagatac catgttttgg cctaccaaaa taaaatggcg
    17761 gaaactcttg cagcgtctga tatgtgtatt tcgcgtgcag gagcctcgac aatcgcagag
    17821 ctaacagcaa gaaagcttcc ggcgcttttg attccatatc ctcatgcaac tgagaaccat
    17881 caattctaca acgcaaaagc tcttcttgat gccggctgtg ttatgtatct aaatgacgaa
    17941 gcagtatcaa gtcccgaatt taaaaaggca gttgttaaac taattgacaa tgacgatatg
    18001 cgcgaagaca tgcatgaggc ttattcaaaa tttgacgctg aaaattctgc taaaaaaatt
    18061 gccgacataa tagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcttatt
    18181 caaaatttga cgctgaaaat tctgctaaaa aaattgccga cataatagaa aatctttaaa
    18241 caaaataata aataatcaac cacgaataaa ctcccgctgc ccccggcccc aggcgatatg
    18301 gtataattac tggtaattat ggggaaaact catataaata gtgtgcattt tattggtatt
    18361 ggtggcattg gcatgagtgg cattgccatt gttgctaacg ctcaaggcat gaaggtgagt
    18421 gggtcagatc ttcgtagttc gaatttgacc gatgagctta agagccaagg cataaaaatt
    18481 tacatcggaa atagatctga aaatattgga acgggcgaca ataaacccga tgttgtggtt
    18541 gtatcgactg cgataatgga acacaatcca gaacttaagg cagccaagga agcaaaaatc
    18601 ccaatttggc acagggccga actcctggcc taccttggtc gcaataaaaa aactttagct
    18661 gtgactggaa ctcacggaaa gacctcaact tcgtcgatgc ttgcaagcgc gcttgagcaa
    18721 attggcgacg acccaagctt tttaattggt ggagttgtta agccatataa cactaatgca
    18781 aaaagtggcg aaggcgagca ctacgttgtt gaagccgacg aaagcgacaa gtctttcact
    18841 tatttaagcc cttatgctgc aattatcaca aacattgaag ctgatcattt ggatcattat
    18901 aaagacatgg atgaaattta cgagaagttt gagactttta tcaagcttgt tccggataat
    18961 ggtttggcag tttgttctgg cgacgataaa aacctaaccg agatcacaaa acgttctgca
    19021 aaaaatgtta tcacttatgg tttcggtgaa gattgtgatt gcatcctttc aaattacaaa
    19081 acaaaaggga ccggttgcga gttcagtttg acgcttccag tttttaatgt tggaaattca
    19141 aatcgcactg aagaggcgaa gtttgattgc tcgcttccat ataaccctgg aattcattac
    19201 gcagaaaatg ctgcaagtgt agttgttttg cttgatgctt tgggttttga tgcttcgcaa
    19261 gttgtggacg ctcttaaaaa ctttggagga attaagcgtc gcttcgatgt tttgggttgc
    19321 gaaggcggag tcaccgttgt tgacgattac gctcatcatc caaccgaaat tgctgcaact
    19381 gtcaaggcgg catttgaact tgattttaaa aacgttcatg ttgtttgaca gcctcacaga
    19441 tttagcagaa ttggactttt ccgagatatt ttcaacaaag aatttgcgca tgcttttgat
    19501 ggctgcaaaa ctctaacttt tacaaacgtt tatggagctg gcgaagtgcc tgttcagggt
    19561 gttaccggct atacattttt ggaaatagtt aaaaacgatg ctggcaaaaa ttcgccaaaa
    19621 acatattatg ttccgcacat gttagacatt atcgaccaca tttctgctat agttgacaat
    19681 ggtgatttag ttattacgat gggcgctggc gatattactt cggtcggccc acaaatttta
    19741 gaagcaatta aaaacaagta ggcgagatta gattatggaa gaattaaaaa tagaaggtta
    19801 taacgctcga attaaaaaag acgagccaat gtctaatcac actacttatc acattggtgg
    19861 tcttgcaaaa tatttcgtct atgtaaatga cgctcaagct ctaaaagaaa ctatcgaata
    19921 tggaaaagag cgaggaatta aatatacaat tgttggagat tgctccaatt ttttagttag
    19981 cgatgatgga tttgacggag ccataattaa attggctggc gacttccgaa aattcagctt
    20041 cgatctgaat cgaaacattt gcactgttgg agctggtgtt aagacagcaa aaatttataa
    20101 tgaattccaa aagaattatt ataagggcct tggctattgc gttggaattc ctggaaccat
    20161 tggcggtgcc gttaagatga atgcgggccg aaagcgccag tgaatttctt cgtcacttac
    20221 aagtgttcaa attatgaata aagatgcaga ggttgagaat ttgaaagcaa ccgatcttga
    20281 atgaggttac agattctcgc caattaaaga tgacgaaatt gttttgcaag ttaatatgaa
    20341 atttaaacca acgaagtccg aaaacgaacg cgagtatatg cgaaacagag caaagctcat
    20401 gacaatggaa agacgttcgc atcaaccaat tggttcgcta agttgcggaa gtgtttttaa
    20461 gaatcctcca gataattttg ctggaaaact tattgaagaa gctggcttta aaaatgcaaa
    20521 agttggtggt gctcaggtat ctgatgttca ttcgaacttt attgttaaca cgggaaatgc
    20581 aaaagctgct gacgttgttg agttaattcg tcaaattcaa aagaaagttt atgatcaaaa
    20641 taagattaaa ctaattccag aggttcaatt cttaggcttt aaggaaccag taagcctttt
    20701 ctaaaatgcc aaacatgcgt aaaaaaaata aagtcgacat gagcgatgtt gaattcgaca
    20761 ctaaaaatcc aacattggtc aaaagcgaaa aagcgaagcg caaagaagac cgcaagaagc
    20821 gtaaaaggtt tcgcgtaatt cgaaatgtta ttcttgtcat cgttgctgtt ttgcttgttt
    20881 ctttgattgg ttcatacatt tatctttatt cgaccgatgc ttttccaatt gaaaacgttc
    20941 aagttaatgg tgttgagcac ttgaccgatg atgaaatgtc tcagttaatg gatatccccg
    21001 aagacacaac cctgcttaaa gttgataccg aaattatcaa gaagcgactt aaacgcgatg
    21061 catgaattca agatgttgat attgttttgt cttttccaaa cactcttgtt atcaatgtta
    21121 ctgagcgaag cgtcacggca attgtcgaag tcccggtctc gtcttcggat aagacgatgc
    21181 gaaattgggc tatttcttcg gaccatatgt gacttatgcc tgtgccggat aaaaattccg
    21241 aagccgcaaa aaacatcagc cagcaaattt ttgcagatgt agaaggagtt atgcacattg
    21301 ttgacgttag cccttcggtt caacctgaaa ttggttctta ttgcagcgat gacccggtca
    21361 ataatgcaat tgatgttgtt gccgccatga caaccgattt gaaaaatcaa gttaaatacg
    21421 ttaaagcttc tgatgcaaat tcaacaaatt tgtttctaaa taatggagtt gaaattgcgg
    21481 ttgggacttc ggagaatctt cgtgaaaaag aacgcgttgc cgaagaactc ctctcaaaat
    21541 atgaaggcaa aatttcttac atcaacgttc gaaacgcaag caatccaact tggagaagca
    21601 tttaatggca aatcaggata aaattttaat gtctcatggc tctggcggtt ctatgatgag
    21661 tgatcttatc gaggccgaat ttgttaacgc ttatattgac aaagatttat atcttggcga
    21721 cgatgcaaca gttcttgaca aaatgaagct tggcgacggt gaaaaaattg ccatgtcgac
    21781 cgattcttat gttgtgcatc cattgacttt tccaggagca gacattgggc gtttggcagt
    21841 ttgcggcact gttaatgata tttcaaccag tggtgcaaaa cctgttgcgc taagtattgg
    21901 ttttatatta gaagagggcc ttgaaatttc tacccttaaa gatatttgcc atagcatcgc
    21961 aaaatcagct accgaagctg gggtcaaaat tgttacgggt gacacaaaag ttgttgagca
    22021 tggaaagtgt gacggaattt acattaatac ttctggaatc ggaattgttc caaacggtat
    22081 aaatttgagt ggtagaaaca ttaagcctgg tgatgttgtg ctgattagtg gcactatcgg
    22141 cgaccatgga attaccatta tgagctgccg tgaaggcttg aatttctcta ccaatctaaa
    22201 aagtgatgct gctccactga actcccttgt ggccgatgtc ttaaaagctg ctccagatac
    22261 caggtgtttt cgcgatccaa cgcgaggtgg tgtggcttcg gccctcaatg aatttgcagc
    22321 tcaaagcaat gttga
//
LOCUS       DBNCDC010000006        19638 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_719_length_19638_cov_7.529439, whole genome shotgun sequence.
ACCESSION   DBNCDC010000006 DBNCDC010000000
VERSION     DBNCDC010000006.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 19638)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19638)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19638
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_719_length_19638_cov_7.529439"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            32..511
                     /locus_tag="AC04VQ_01040"
     CDS             32..511
                     /locus_tag="AC04VQ_01040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit B family protein"
                     /protein_id="HIO9552837.1"
                     /translation="MDETGVLGLLEELSILIEEAKPVFGKSQMKQVEANTVLEIIDEI
                     REVFPGEFSQARQIVRERQSLLDDAEDEANRMIEDARSQAVTIASEQEIVRLSQQKAD
                     QIMADAREVERQTRAGAEDYADEVFGHVEQSLDSLLNNVRRCRDRLNTNALEETPTE"
     gene            515..1051
                     /locus_tag="AC04VQ_01045"
     CDS             515..1051
                     /locus_tag="AC04VQ_01045"
                     /inference="COORDINATES: protein motif:HMM:NF014657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIO9552838.1"
                     /translation="MAKNKEQLTIHISHDLFKPSAIDTFDGLYNVSKLEGQCDTFNLK
                     EPIKYQITITNTGDAFLITGSAEAEAVTSCSRCLEDVDVKLSAKIDAYYLIEPPETAD
                     ENEINEFEILPDDHNIPLGEIIEATMIVDAPPKPLCAEDCKGLCPKCGTNLNKKTCNC
                     AEQPDESNPFSVLKDFKV"
     gene            1060..3168
                     /gene="ligA"
                     /locus_tag="AC04VQ_01050"
                     /pseudo
     CDS             1060..3168
                     /gene="ligA"
                     /locus_tag="AC04VQ_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013790.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
     misc_feature    3239..3401
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            3441..3967
                     /gene="rplJ"
                     /locus_tag="AC04VQ_01055"
                     /pseudo
     CDS             3441..3967
                     /gene="rplJ"
                     /locus_tag="AC04VQ_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012679.7"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
     assembly_gap    3968..4067
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4180..4557
                     /gene="rplL"
                     /locus_tag="AC04VQ_01060"
     CDS             4180..4557
                     /gene="rplL"
                     /locus_tag="AC04VQ_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIO9552839.1"
                     /translation="MTNEEIIKALEEMPLLQASELVKEIEEKFGVSAAAPVAVAAAAP
                     GAAGGEAGGEEKSSFDVVMDGFGDNKIGVIKAVREITNLGLKEAKDMVEGAPATVLEG
                     ASKDDAEAAKAKLEEAGAAVSLK"
     gene            4764..5579
                     /locus_tag="AC04VQ_01065"
                     /pseudo
     CDS             4764..5579
                     /locus_tag="AC04VQ_01065"
                     /inference="COORDINATES: protein motif:HMM:NF019314.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
     gene            5664..7247
                     /locus_tag="AC04VQ_01070"
     CDS             5664..7247
                     /locus_tag="AC04VQ_01070"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIO9552840.1"
                     /translation="MYTEGHRELDLFRVRIDDETLYVMYINIDQAKMISYSYEKYEMY
                     LPYFTSSMTIFIQIIIFGLLMFAVFVLLKLLVGNNLNKINKSLHEITQGNLDEHVDVY
                     SHKEFATLSDSINATVDTLKQYIEEEANRYKEEFEMAKQIQLSALPNVFPPFPNDPEI
                     DLYAKYKPAKIVGGDFYDYYYIGKNRLAFLIADVSGKGVPAAMFMMRAKTAFRVFADK
                     KDNPADVLEAVNNYLCENNEAEMFVTAWFGMVDITTGKIMCASAGHNPPFLINTKSGE
                     VKCLNYKPSLVLGAMNNIGYNNYIFEMKPDEKLMLYTDGIVEAHDVKNNLYGEERFKK
                     SLEKHKDLSIRDLCENAAFEVDEYSKGQEQFDDYSLLCFTYKGEQEGSGLKNDFWESE
                     ITVFSKIESVNKVMERCEETLKYSNAPANFVQMFDVCIDEIFSNIVKFSYDRPNEYIS
                     VRIVIDETSEKKCVTVTFVDTGPRFNPLEQSAPDITLSAEEREIGGLGIFMVRQMMDD
                     LRYEYKSLANWLTLVKYYD"
     gene            7256..7834
                     /locus_tag="AC04VQ_01075"
     CDS             7256..7834
                     /locus_tag="AC04VQ_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552841.1"
                     /translation="METQSKIKLSKIVIIASIISAILLAITAVIPSVADSIKSNANGE
                     DVGVTATKTAQWTDEDFTKGNVQITAKATGKDEYIPNVLFIGTLCNAHGLTNETIVHS
                     LNAIKEFCDVDYFLCHNADPSRTDLDAKGSIKKGDPDVSTDIVPEMVVSDHQALQKFL
                     RHIYEEHNNMVQKTGKGYDFIVLEFDGTRISD"
     gene            8024..8644
                     /locus_tag="AC04VQ_01080"
     CDS             8024..8644
                     /locus_tag="AC04VQ_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552842.1"
                     /translation="MNVRTQNLDTRPINYNSLKGLLAPDLYVSTTEHAGMTDEEYRKI
                     EANLSTDPFDADHTLKFDRQIGYKSSSAVGGFLKDAIDSIFEYQIVIKDKIDLKDNLK
                     ITTDSVVFEMRDKDGDDWAVVPASMKPVVTVDFKTGEVNASVAKLRGDKQFRVTIGFE
                     DKSSTERFIDVSKDGNPNDGNAEVEVIPGTGVTPEPATPGTEIPLD"
     gene            8744..9076
                     /locus_tag="AC04VQ_01085"
     CDS             8744..9076
                     /locus_tag="AC04VQ_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552843.1"
                     /translation="MPKGSEATETSTIKFQYWSTVIDGVDTFVSNDFKYTPTPDPTTN
                     RFKSATYIAHFAESPAETKYLTVKKVWSDGDDFHLDDTINVRLHVKNSTGKEAVLEDS
                     IKIDASTK"
     gene            9131..9454
                     /locus_tag="AC04VQ_01090"
     CDS             9131..9454
                     /locus_tag="AC04VQ_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552844.1"
                     /translation="MQYYFTEDPIEGYQTTYEVVTDATKPFEIGNTVITNTYVEPPTP
                     PGPTPDPPTPTPTPDPGNVTPVGYGSVKTGDMVNLVSLLIFAVVGSASIAVITRVRKK
                     NKERG"
     gene            9489..10217
                     /locus_tag="AC04VQ_01095"
                     /pseudo
     CDS             9489..10217
                     /locus_tag="AC04VQ_01095"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
     gene            10217..10648
                     /locus_tag="AC04VQ_01100"
     CDS             10217..10648
                     /locus_tag="AC04VQ_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552845.1"
                     /translation="MFERDYLVRMLVDLAAAIRRALDKSREAKDLEGAVDTLENSITN
                     ATDLDGAVLMTLAPESIASVLQVANTDPRVVIYIAHSMQLQAHYLRQMHNDAVADLRE
                     KQARALASSYKFELPENIDQVDDELFNDEEFLKSIEELCEA"
     gene            10649..10894
                     /locus_tag="AC04VQ_01105"
     CDS             10649..10894
                     /locus_tag="AC04VQ_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIO9552846.1"
                     /translation="MIMLSFDIEEFDVPREKGLNISFEKSMEISRLGFKKILSILKAN
                     NVRATMFCTANFVQNAKDLINEAIRDGHEIASHGVDH"
     gene            10982..11434
                     /locus_tag="AC04VQ_01110"
     CDS             10982..11434
                     /locus_tag="AC04VQ_01110"
                     /inference="COORDINATES: protein motif:HMM:NF023385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HIO9552847.1"
                     /translation="MFPVDENLIEQCGYKYNSSLNPAFIPGKYMHLDVPRTAFMQGKV
                     LQIPASVTKTRIPMFWLSLHTFPFYAYKKMAKQILKSDGYFMTYFHPWEFVNHPKEIR
                     KYMLPIVNHNNGTNMSLRLDRLIKYFKAQGQDFVTFSDFMLSYKQKGK"
     gene            11435..12154
                     /locus_tag="AC04VQ_01115"
     CDS             11435..12154
                     /locus_tag="AC04VQ_01115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIO9552848.1"
                     /translation="MATKLAIICPCFNEEEVIESSAKKLFSIIDGLVQKNKIDQSSTV
                     VFVNDGSFDTTWQKITKLHAAESRLKGINLAFNSGHQNAIMAGMMAVKDQVDAAITID
                     VDLQDDLNCIEKMIDLFESGNDVVYGVKTKRKADSLKKRVTARVYYRVQKMMGINIVD
                     NHADFRLVSNNVLKTLSKFHESNLYLRGIIPSLGFKSATVDDKISERSAGKSKYTSKK
                     MMKLATDGITSFSSTPLKVVF"
     gene            12170..12379
                     /locus_tag="AC04VQ_01120"
     CDS             12170..12379
                     /locus_tag="AC04VQ_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552849.1"
                     /translation="MIVALINGIDIIVALCNGTAQPGWSQLMLSVWFVGGVVMVALGM
                     VGIYIGRIYSEVKHRPQYIISEVLD"
     gene            12380..13447
                     /locus_tag="AC04VQ_01125"
     CDS             12380..13447
                     /locus_tag="AC04VQ_01125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIO9552850.1"
                     /translation="MALNNKNKINMGRPRPSVRQEQFHVKNVVTPDTKEAARRAEERR
                     KAKDARVLGRSLNRKSERDFNIKGSIFGSINSNVKRGIVIGLIAALVILIGVAVGVFV
                     FMASTSAKLSIDDEARAKLVRVQENQEYYMLFAADVDSDENEAPDMLMLTRVDPLKMS
                     IIMVCIPSSTYVAAAQGGGTTLENVYKSSGDAGLIEAVASFSEVGITHYVKASAEGLS
                     NLVDAFGGIDVFLKEYVDDVNVGDVYIGQGDQHIKGYETITLLKSNNFNGGMTTVMDN
                     QKQVCMGILKAALFSKQVNIAVLTDQIAGSIKTDMGAGDIINFVDYYSKIEEDKIMST
                     DVPGYKTMRNNMMVFMIDASS"
     gene            13478..13807
                     /locus_tag="AC04VQ_01130"
     CDS             13478..13807
                     /locus_tag="AC04VQ_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HIO9552851.1"
                     /translation="MILTTEDDLTYKDIDPSSFTIVIENGAGIDGAASSLADKLKGMG
                     FNVIDSHNADSNIYKETLIIYGEASMKTNAIAIKNSINNGRVVNGEGLYNMKSDILII
                     IGADLKI"
     gene            13818..14048
                     /locus_tag="AC04VQ_01135"
     CDS             13818..14048
                     /locus_tag="AC04VQ_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HIO9552852.1"
                     /translation="MALNKEDVQAVLDLVRPSLQADGGDVSLVDVADDGTVTVQLEGA
                     CKGCPMSQMTLANGVERILKERIPEVTKVVPA"
     gene            complement(14512..14661)
                     /locus_tag="AC04VQ_01140"
     CDS             complement(14512..14661)
                     /locus_tag="AC04VQ_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552853.1"
                     /translation="MIHGLKPTHNIAHKNVIVATKGLVIFFAIKPVKITEAKTHNTDK
                     ITKRK"
     gene            14843..15058
                     /locus_tag="AC04VQ_01145"
     CDS             14843..15058
                     /locus_tag="AC04VQ_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552854.1"
                     /translation="MISNHKEIIKKYSKVNLVKVMICILVALFGFGFASVASGKISNY
                     GVQIDKDRSFTATHFLMMGINLENHGG"
     gene            15074..15226
                     /locus_tag="AC04VQ_01150"
     CDS             15074..15226
                     /locus_tag="AC04VQ_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552855.1"
                     /translation="MISRSYPNVAEREAGNIREWQHRLFTYGIPDISFILLNKTLTNF
                     NDGSFA"
     gene            15362..15535
                     /locus_tag="AC04VQ_01155"
     CDS             15362..15535
                     /locus_tag="AC04VQ_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552856.1"
                     /translation="MILIGCIFTFLNKKPKREEFVAYLSILAISAFLTVCEPDPRYLL
                     LYLPYFVLIAPKG"
     gene            15606..15740
                     /locus_tag="AC04VQ_01160"
     CDS             15606..15740
                     /locus_tag="AC04VQ_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552857.1"
                     /translation="MAATNNKLKLGACVMILIGGMFGSAIFSLSGLTIANAGPASLIS
                     "
     gene            15759..16910
                     /locus_tag="AC04VQ_01165"
                     /pseudo
     CDS             15759..16910
                     /locus_tag="AC04VQ_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
     gene            16946..18232
                     /gene="murA"
                     /locus_tag="AC04VQ_01170"
     CDS             16946..18232
                     /gene="murA"
                     /locus_tag="AC04VQ_01170"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012798639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIO9552858.1"
                     /translation="MNRKDDIIVVQGNQTLTGKVAVSGAKNSALKLIAASILVNDVCT
                     IKNVPNISDIDIMCQVLESLGAKVDRKDHTLVIDSSSINSVEASYKLVSQMRASIAVL
                     GPLVSRFGEAKVAMPGGCQIGARKIDMHLMGLRQLGVKFDVNFGYLNATVPSKLRGAN
                     IRLDFASVGATENILMASVCADGRTIIDNAAREPEIVDLCNFLNEIGCSIHGAGTSVI
                     EIEGVDVNSLHGCTYKTCGDRIEAGTYIVGGAATGGPLTVEGIDPDYLKTAIRKLRDM
                     GVDVKIDKDSITVSRDVTKPLRTVQVQTLPHPGFPTDLQAQFMLLACLADGTSIITEN
                     VFENRFMFAAEIMRMGANISLQEHHAVVKGPVKLQGAPVSSTDLRAGASLVLAGIIAE
                     GTTTVTEVGHIDRGYEDFVDKLCRLGAEIKRKNPKK"
     gene            18374..19153
                     /locus_tag="AC04VQ_01175"
     CDS             18374..19153
                     /locus_tag="AC04VQ_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIO9552859.1"
                     /translation="MADQESKHYTGEDIQVLEGLEAVRKRPGMYIGSTGPRGLHHLVY
                     EVVDNAVDEALAGYCENIDVTIHKDNSITVVDDGRGIPVDIHPKEKIPTVEVVLTILH
                     AGGKFGGEGYKVSGGLHGVGVSVVNALSSKLVAEVSRDGKKYTMECERGKTTQKLKEA
                     GKSDKSGTVISFWPDPEVFTETTEYDYDTLATRFREMAFLNKGLKIHLKDERSDKENS
                     FCYDGGIIDYVEFLTKGKETLNKPIYFTAQNEDGDVEIAMQ"
     gene            19181..>19638
                     /locus_tag="AC04VQ_01180"
     CDS             19181..>19638
                     /locus_tag="AC04VQ_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HIO9552860.1"
                     /translation="MAFANNINTHEGGTHLDGFKQALTRVVNNYARSKGILKEKDSNL
                     SGDDCREGLSAIISVKLHDPQFEGQTKTKLGNTEIRPLVQNAVADKLASYLEENPGPA
                     KKIVSKASQALKAREAARKAREATRRKGVLDSFSLPGKLADCSSKEAEKS"
BASE COUNT         5987 a         3183 c         4214 g         6154 t
ORIGIN      
        1 tgataaacgt agtttaaagg aaggaaaaat catggatgaa acaggagttt taggtctctt
       61 agaagaactc tctatcttaa tcgaagaagc taagccagtt tttggtaaaa gccaaatgaa
      121 gcaggtagag gctaacaccg tacttgaaat cattgatgaa attcgtgaag ttttccctgg
      181 cgaattctct caggctcgtc aaattgttcg cgagcgtcag agtttgctag acgatgcaga
      241 ggacgaggcc aacagaatga tcgaagatgc ccgtagtcaa gctgtaacaa ttgcaagcga
      301 gcaagaaatt gttcgtttgt cgcagcaaaa ggccgatcaa attatggctg atgctcgtga
      361 agttgagcgt caaactcgtg ctggtgccga agattatgcc gatgaggtat ttggccatgt
      421 tgagcaaagc ttagacagcc ttcttaacaa tgtccgcagg tgccgtgatc gtctaaacac
      481 caacgccttg gaagaaacac caactgaata gttcttggca aaaaataaag aacagttaac
      541 tattcatatt tcgcatgatt tatttaagcc gtcagcgatt gatacgtttg acggcttata
      601 taatgtctca aagctcgaag gtcaatgcga caccttcaat ttaaaagagc caatcaagta
      661 tcaaattaca attacaaata ctggcgatgc ttttctaatt accggaagcg ccgaagccga
      721 ggctgtcact tcgtgttctc gatgcctcga agatgtagac gttaaactta gtgcaaaaat
      781 tgatgcttac tatttaattg agccgccaga aactgccgat gaaaatgaaa ttaacgaatt
      841 tgagattttg ccggatgatc acaacattcc gcttggtgaa attatcgagg ctactatgat
      901 tgtggatgct ccaccaaagc ctctctgtgc ggaagattgc aaaggtcttt gtccaaaatg
      961 tggcacaaac ctcaataaga aaacttgcaa ttgcgcagaa caaccagacg aatctaatcc
     1021 cttctcagtt ttaaaagatt ttaaagtata ataaaaaaca tggaagatct atttagttct
     1081 gtaaatattg atgctgatat tgacgtcaat tcgctatccg aaaaagaggc gaaggtaaag
     1141 attgatttgc ttcgtaagga aattgaacat catacatatt tatattatgc gcaggatgaa
     1201 cctgaaattt ctgatggtgc ttatgattcg ttgatgcgag ctcttcgtaa gttagaagat
     1261 gcgtttccgc aatttcaaga tgcaaattct ccaacaatgc gtgttggtgg ttatgttggt
     1321 gaggcttttg cccctgtgca acatcaagtc aaaatgtatt cgctcgataa tgcgatggat
     1381 ttgaatgaac ttgacgattg gatgcaaaag gtcgaagatg agttcggcga atatgttcct
     1441 ctaatgtgtg agcttaaaat tgatggatct tcaattgcgc taacttatga aaatggtcaa
     1501 cttgttcgtg ctgcaactcg aggtgatgga acaactggtg aagatgttac tgcaaacatg
     1561 cgaactgttc gtgatgtgcc gttgaaatta cgcgctgaaa atttggaagg cacaattgag
     1621 cttcgtggcg aagtttatat gccgaagcaa agttttgaaa ctttaaacga agctgctgtt
     1681 gccgagggta atcagaaatt tgcaaatccg cgtaatgctg ctgctggatc tattcgtcag
     1741 aaggatccaa caattacgaa gaatcgcgac cttgcaacat ttatttatgc gattgctgat
     1801 gttgagcctg ttagttctga agttcaaagt caagaaagct tacttaattg attaaaaagt
     1861 tctggctttc atgtaaatcc agatgttaaa ctttgcacaa atcgcgatga ggttaaacaa
     1921 ttttgcaaag atgctattga gaagcgtgac aaattaccat acgaaataga cggagttgtt
     1981 gtaaaggtta atgattttaa caaacaaaat cttatgggct ttacttctcg tgctccgaag
     2041 tgggccattg cttttaagtt tcctcctgag gaaaaggcta ctctgctaaa agacataaca
     2101 gttcaagttg ggcgcaccgg cgttttgact ccagttgcag aattggagcc agtaagtttg
     2161 gctggaacaa ttgtgagtcg tgcaacctta cataatttag atgaggtgcg tcgcaaggac
     2221 gttcgcatcg gtgacactgt aattgttcat aaagcaggtg acatcattcc tgaagttgtt
     2281 ggtcctatta tgagtgcacg tactgctgat gcaaaacttt gggagatgcc aacaaattgt
     2341 ccaagttgcg ggagtgaagt tgttaaagat cctgatggcg ttgccattcg ttgcgtttca
     2401 gcagaatgtc caaaacaaat tcatgagcgt tttgttcatt gggtaagtcg taaggcattg
     2461 gatattgatg gcatgggaac tgaaataatt agtggtctta ttgaatctgg gctggttcat
     2521 gatgttgctg atatttataa acttacagag ggtgaaattg cttcgttgtc tcgtggacgt
     2581 caaaacgtcg aaggcgaaga cattcgtgtg ggtcatacca tcgcagcgaa gctaattagc
     2641 gctattgaag atagtaaaaa gcaaagtttt gcacgtgttt tacatgggct tggcattcgt
     2701 aatgttggcg agaacgcagc agaactttta gttcaacatt atccagtttt cgaagagcta
     2761 gaaaaagcta gtactgatga tttatcaaaa atcgaaggta tcggcccaat catggctgaa
     2821 aacatacact ctttctttaa gaatgatcag aatcttgatt taattcatcg tcttcagacg
     2881 gcaggtcttt gctttgatga cagcgctcga atcagcgaag ctcaatcggg cgaccaacct
     2941 cttgctggaa atacttatgt tttaactggc actttagtag aaagtggttt aagccgcgac
     3001 gaagcaggcg caaaacttaa agcgcttggt gcaaaagttt caagtagcgt gtcttcgaaa
     3061 actacagcag tcattgctgg tgaagcagca ggaagcaaac ttacaaaagc ccaagaattg
     3121 ggaattcaaa ttctaacaga gcaagacttc ctaaatttaa ttaaatagtt gttgacattt
     3181 aaggattttt tagtaatata tgtgctccgc gcttttagga gtgcggaaat gtactttgag
     3241 aaatacattt ttgctactta agacagcagg taccgcaagg tttaattgag gaagctcaac
     3301 ccgccgaggt gagcgaaaag gtaggccaaa acttacaatt tagtgtattg acacctgacc
     3361 cttcgcgcat cgagtagcgt ggagggtttt ttattaccct caaaatgtca gacttttgag
     3421 gaattttaag gaggtgtaat atgccaaata agcaaaatgt ggaaacttta gccacaatta
     3481 aggatgagct taaagacatc tcggcaatgt ggattgttga ttatcgtggt cttagcgtta
     3541 aggaaattca agagctccga agaaacattc gcgatgctgg cgctaagatg actgtttgaa
     3601 aaaacacaat catgaaactt gccattgacg aactcaaact tccagattta ggtgatgttt
     3661 taactggtcc aagtgcattt gttgttgcgc ctgaagatcc agttacaagc gcgaaagcaa
     3721 ttcgcgactt tgctaagaaa aacgaagcct tagaaattaa aggtggagtt atggacggcg
     3781 agtttgttga tgctgatggc gttaaagcta tcgcagctct tccaagcagg gaggaagttt
     3841 acgctcaaat tgccagcatg atttctggtg ttgcgcgtgg cttggctact gcaatcaatg
     3901 gtgtacctcg tggtattgca caaacaatat ctcaggtcgc cgaacaaaag cccgcagatg
     3961 cggcatannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngta ttgcacaaac
     4081 aatatctcag gtcgccgaac aaaagcccgc agatgcggca taaaaaaatt tgtaaattgt
     4141 aaatttgccg cataagcggc ttgattgaaa ggaaataaaa tgactaacga agaaattatt
     4201 aaagctttag aagaaatgcc tttattacag gcttctgaac ttgttaaaga aattgaagag
     4261 aaatttggag tttctgctgc tgctcctgtt gctgtagctg ctgctgctcc tggtgctgct
     4321 ggtggagaag ctggtggcga agaaaagtca agctttgacg ttgttatgga tggcttcggc
     4381 gataacaaaa ttggtgttat caaggctgtt cgtgagatca caaatcttgg tcttaaagaa
     4441 gcaaaagata tggttgaagg tgctcctgca acagttttgg aaggcgcaag caaagatgat
     4501 gctgaagccg caaaagcaaa attggaagaa gctggtgctg ctgtatcttt gaagtaatta
     4561 tcttcaagaa attaaaaaaa tgacctgtcg aatttcggca ggtctttttt tatgcaaaaa
     4621 tctcaaaata tacctttaac catattcttt gttatgcttt gtgctataat aggccatccg
     4681 caaaaattta aaagtaggga gcgtaagtgt cattatttat atctggaata attacggttg
     4741 gaattaccgt tggcgcattc tatatggata aaaagaccag cattggtaag cttaaacctt
     4801 tttacaaaca aacagtttat ggccttatgt ttgctatggc agcaattttt gcaactgaac
     4861 ttggtggcca aaatttaggt ggtgctttaa ttaatgctcg tgatgccgca attgtagttg
     4921 ctggcctttt atttggtcct tgagcaggtg tcttcgctgc tatttgagct gctgctgaaa
     4981 gatattttgt tgctacattt ttatttggtg ttggaagcta tactcaaata gcttgcacca
     5041 ttgcaacatt atttgctgga tgtttctcgg ctcttttcta tattttaatt tttagaaaac
     5101 atcgtccata tgttattcat gctgtggttt ttgtaataat taccgaagta gttcacatga
     5161 ttttaattcc acttacgcat ttgggcgatt tagaaacagc aaacatgtta atgatggaaa
     5221 tcttccttcc aattttggtc tcgaacattt taattgctgc ggctgctgtg ctttgtactt
     5281 cgttgatgga aattagattt agcgggcata ggattcgaga tgttgttaaa gaaaaagata
     5341 ttttattttc ccaagatttg ttctattcat ttcaattttg gttgttactt gtaattattg
     5401 ttgcatttgc agctaacatt ttaatcatct ctcgtttgta tggagttatg tctttggagt
     5461 cttcgtcaaa caaatatgag agtgaactaa aaattatgaa gtccttaatt tatcaagact
     5521 atggatattt tcataatttt aatccagatc aaattgcatg tgtttatgca gagcaatgat
     5581 cagatgaaaa agttgttgct tttgaagttg ctgataaaga caacaatata attagcaaca
     5641 atgcaaagtt cccagatctt ggaatgtata ctgaagggca tcgtgagctt gatcttttca
     5701 gggttcgaat agacgatgaa accttatatg taatgtatat caatattgat caagcaaaga
     5761 tgatttctta ttcatatgaa aagtacgaaa tgtatttgcc ttactttacg tcttcaatga
     5821 caattttcat tcagataatc atttttggtc ttttgatgtt tgctgtgttt gttttactga
     5881 agcttttagt aggaaataac ttgaataaga ttaataagtc gctgcatgaa attacgcaag
     5941 gaaatttaga tgagcatgtt gatgtttatt cgcataaaga atttgcaact ttgtctgaca
     6001 gtattaatgc aacggttgat actcttaagc aatatatcga agaagaagca aaccgctata
     6061 aagaggaatt cgaaatggcg aagcaaattc agctttcggc tttgccaaat gtattccctc
     6121 cattcccgaa tgatccagaa attgatcttt acgctaaata taagcctgca aaaattgtag
     6181 gcggtgactt ctatgattat tactacattg gtaaaaacag gttggcattt ttaattgcgg
     6241 atgtttcagg aaagggcgtt cctgctgcta tgtttatgat gcgtgcaaaa acggcgttca
     6301 gggtcttcgc tgataagaag gataatccgg ccgatgtttt agaagcagta aacaattatc
     6361 tttgcgaaaa caacgaagcc gagatgttcg ttaccgcctg gtttggtatg gttgatataa
     6421 ctactggaaa gattatgtgc gcttctgctg gtcataatcc gccattcctt atcaatacta
     6481 aatctggcga agttaaatgt ttgaattaca aaccttcgct tgttttgggc gctatgaata
     6541 atatcggata caataattac atctttgaga tgaaacctga tgaaaaatta atgttatata
     6601 cagatggtat tgttgaagct catgatgtga aaaacaatct atatggcgaa gagagattta
     6661 agaagtcctt ggagaagcat aaagatttgt ctattcgtga tctttgtgaa aatgctgctt
     6721 ttgaagttga tgaatattct aaaggccaag agcagttcga tgattattca ctgctttgct
     6781 ttacttacaa aggtgagcaa gaaggaagtg ggcttaagaa tgacttctgg gaaagcgaga
     6841 ttacagtttt ctctaagatt gaaagtgtaa ataaggttat ggaacgttgt gaagaaacat
     6901 tgaaatatag taacgctcct gccaacttcg ttcaaatgtt tgatgtttgt attgatgaaa
     6961 tttttagtaa cattgttaag tttagttatg acagaccaaa cgaatacatt tcagtacgaa
     7021 ttgtaattga tgaaacatct gaaaagaaat gtgtaacggt tacttttgtt gacactggtc
     7081 cacgctttaa tccgcttgag cagtctgccc cagatattac acttagtgca gaagaacgcg
     7141 agattggtgg acttggaata tttatggttc gtcagatgat ggatgacctt cgttatgaat
     7201 ataagagttt agctaattgg cttactttgg ttaaatatta tgattaggag attcgttgga
     7261 aacccaatct aaaataaaac tttcaaaaat tgtaataatt gcgtctatta tttctgcaat
     7321 tttacttgca ataaccgctg taattccatc tgttgcagac tctataaagt caaatgctaa
     7381 tggagaagac gttggtgtta ctgctacaaa aacagcgcaa tggaccgatg aagattttac
     7441 aaagggcaat gttcaaatta ctgcaaaagc tacaggaaaa gatgaatata taccaaatgt
     7501 attatttatt ggtacccttt gtaacgcaca tggtttaacg aatgagacaa ttgttcattc
     7561 gttaaatgct attaaagagt tttgtgatgt tgattatttc ctttgccata atgctgatcc
     7621 ttcaagaact gatcttgatg ctaaaggaag tattaaaaag ggtgatcctg atgtctcaac
     7681 tgatattgtt cctgaaatgg ttgtgagtga tcatcaggct ttgcaaaaat ttctaagaca
     7741 tatatatgaa gagcacaata atatggtgca gaaaactggc aagggttacg attttattgt
     7801 tttagagttt gatggtacaa gaatttctga ttgatataga tctttaatac ctgatggtga
     7861 tacagattca cgtagacctt ctggtgatta tacaaaatat gttaatgaag attcagtggt
     7921 cgatattctt aaatcatatt atgcaaataa tcgagttatc tgaattactg ataacgaagt
     7981 tgacgtaact gacccaaata ctggttatca tccaagtacg gcaatgaatg ttagaactca
     8041 aaatttagac actcgtccta taaattacaa ttctctcaaa ggcttacttg ctccagattt
     8101 gtatgtttcg actactgaac acgcaggtat gacagatgag gaatatagaa aaattgaagc
     8161 taacttgtcg acagatcctt ttgatgcaga tcatacttta aaatttgata gacaaatagg
     8221 ttataaatct tcgtcagctg ttggaggatt tttaaaagat gcaattgatt ctatctttga
     8281 atatcaaata gtaattaagg acaaaattga tttgaaagac aacttaaaga ttaccaccga
     8341 ttctgtagtt tttgaaatga gagataaaga tggtgatgat tgggcagttg ttccagcctc
     8401 aatgaaacct gttgtcactg ttgattttaa aacaggagaa gtaaatgcaa gtgttgctaa
     8461 gcttcgagga gataaacagt ttagagtaac tatagggttt gaagacaaat catcaactga
     8521 aagatttatt gatgtatcaa aagatggtaa tccaaatgat ggtaacgccg aagtagaggt
     8581 aattcctggc actggtgtta cacctgaacc tgcaacacct ggaaccgaaa taccacttga
     8641 ttgagacatt ccaatctatt acgttgtaga agacgcatat aaagaaacag aaggaattaa
     8701 agttacaaat cctgaagata atattacgac aagttctaaa tcggtgccaa aaggttccga
     8761 agccacagaa acttctacga taaaatttca atattggtca acagtcattg atggtgttga
     8821 tacatttgta agtaatgatt ttaaatatac cccaacaccg gatccaacaa caaataggtt
     8881 taagagtgca acttacattg cgcattttgc tgaatctcca gcggaaacta agtatttgac
     8941 tgttaaaaaa gtttggtctg atggtgatga ttttcactta gatgacacta ttaatgttcg
     9001 tcttcatgtt aagaatagca ctggaaaaga agcagttctt gaggatagta tcaagattga
     9061 tgcttcaaca aaatgaactt ataaaactaa atttgaatat caagtttacg aagctgatag
     9121 aaaaactcct ttgcaatatt attttactga ggatcctatt gaaggttatc agactactta
     9181 tgaagttgtt actgatgcca caaaaccttt tgagattgga aatactgtta taacaaatac
     9241 ttatgttgaa cctccaactc ctccaggacc tacaccagat ccgccaactc ctactccaac
     9301 gcccgatcct ggaaatgtaa ctcctgttgg atatggttct gtaaaaactg gtgatatggt
     9361 taatcttgta tcgttgttaa tttttgctgt tgttggtagt gcttcaatag ctgtcataac
     9421 tagagttcgt aaaaagaata aggaaagagg ctaacatgtc aggacattca aaatgagcta
     9481 caaccaaaca tcgtaaagct gcgcaagaca aaaagcgttc agcgcttttt agcaagcttt
     9541 cgaaaaacat tactgttgct gcaaaaactg gaggagatcc taatccagaa aataatgctt
     9601 cgcttgctgc tgcagttgaa aaggcgaagg caaattcgct tcctaaagat aagattaagc
     9661 aagctataga taaagctttt ggttctggca aggatgcagc aaattacgaa gaagtagtct
     9721 acgaaggtta tgggccttgc ggtgtagcag ttttatgcga agctttgact gataatagaa
     9781 atagaactgc tgccgatgtt cgaagtgctt ttactcatag cggtggtaat cttggaactt
     9841 caaacagcgt tgcttttcaa tttgagcgca aaggtgaaat tgttattgaa aagtctgctt
     9901 gtgctgacga agatactttg atgatggctg ttgcagatgc cggtggcgaa gattacgaag
     9961 acgccgaaga agagtggatt gttaccactg acggcaacac aatgatgagt gttaagaatg
    10021 ctctagttga agctggtatt gaagttaagg gtgccgaggt tgtaatggag ccaaccaatc
    10081 ctgttgacgt tgatcctcaa caagctaaaa aagttatgcg tttgattgac aacttggaag
    10141 agcttgaaga tttgcaaaac gtttatcaca caatgaatat gaccgacgaa atccttgctg
    10201 ctttagacga ggaataatgt ttgaacgaga ttatcttgta cgaatgcttg tcgatttagc
    10261 tgcggctatt cgacgtgctc ttgataaatc tagagaagca aaagacctcg agggtgcagt
    10321 tgacacctta gagaattcaa tcacaaatgc aacggactta gatggtgcag tgcttatgac
    10381 attggcacct gagtctattg cttcggtgtt acaagttgca aacactgatc cacgagttgt
    10441 aatttatatt gcacattcaa tgcagttgca agcgcattat cttcgtcaaa tgcataatga
    10501 tgctgtggct gaccttcgtg agaagcaagc gcgcgcattg gctagttctt ataagtttga
    10561 acttccagaa aatattgacc aagttgatga tgaattattc aatgacgaag aattcttaaa
    10621 atctatcgaa gagctctgtg aggcttaaat gataatgctc agttttgaca tcgaagagtt
    10681 tgatgtccct cgcgaaaagg gtcttaatat cagttttgaa aaaagtatgg aaatttctag
    10741 acttggtttt aaaaaaattc tctctatctt aaaagcgaac aatgttaggg caacgatgtt
    10801 ttgcactgca aattttgttc aaaatgcgaa ggatttaatc aacgaagcta ttcgcgatgg
    10861 acatgagatt gcaagtcatg gtgttgatca ttgaaaccct aaagctgatg acccaaaaat
    10921 ttcaaaggag ataattgaaa ctgagattaa ccgagagatt gttggatata gacaacctcg
    10981 aatgtttcca gttgatgaaa atttgataga gcagtgtggt tataaatata attcttcgct
    11041 taaccctgct tttattcctg ggaaatatat gcatttagat gttccgcgca ctgcttttat
    11101 gcaaggaaag gttttacaaa ttccagcatc tgttacaaaa acaagaattc ctatgttttg
    11161 gctttcgctt cacacttttc cgttctatgc ttataaaaaa atggcaaagc aaattttaaa
    11221 atctgacggt tattttatga cttattttca tccttgggaa tttgttaatc atcctaaaga
    11281 gattcgaaaa tatatgctgc caattgttaa tcataacaac ggaactaata tgtctcttcg
    11341 tcttgataga ttgataaagt acttcaaagc ccagggtcaa gacttcgtta cctttagtga
    11401 ctttatgcta tcatataagc agaaggggaa gtaaatggct acaaaacttg caataatttg
    11461 tccttgtttt aacgaggaag aggtaattga gagcagcgct aaaaagttgt tttcaattat
    11521 tgatgggtta gtgcaaaaaa ataaaattga tcaatcaagc acagttgttt ttgtaaacga
    11581 tggatctttt gatactactt ggcaaaaaat tacgaagcta cacgcagctg aatctcgcct
    11641 gaaaggaata aatctagcat ttaatagcgg gcatcaaaat gcgataatgg ccggaatgat
    11701 ggctgttaaa gaccaagtag atgccgcaat tacaatagat gttgacttac aagatgactt
    11761 aaattgcatc gaaaagatga ttgacctttt tgaatctgga aacgatgtag tttatggtgt
    11821 aaaaacgaag cgaaaggctg acagtttaaa gaagcgtgta acagcacgag tttattatcg
    11881 cgttcaaaaa atgatgggaa ttaacattgt tgataatcat gctgatttta ggttagtaag
    11941 caataatgtt ttaaagactc tttctaaatt tcacgaaagc aatctttatc ttcgaggaat
    12001 aattccttcc ctgggtttta aatctgcaac tgttgacgat aaaattagtg agcgaagtgc
    12061 tggcaaatct aaatatacaa gtaagaagat gatgaaactt gcaacggatg gaatcacaag
    12121 tttttcatcc actcctttaa aagttgtttt ttgaattgga attatatttt tgattgttgc
    12181 tttgattaat ggcattgata ttattgttgc tctgtgcaat ggaactgcac agccaggttg
    12241 gtcacaattg atgttgagtg tttggtttgt cggcggagtt gtaatggttg cgcttggcat
    12301 ggttggtatt tatattggaa gaatttatag cgaagtcaag catcgtcctc aatatattat
    12361 ttcagaagtt ctagactaaa tggcacttaa caataaaaat aaaattaata tgggtcgacc
    12421 acgtccaagt gttagacagg aacaatttca tgtaaaaaat gttgtcacgc ccgatactaa
    12481 agaggcagca aggcgtgctg aagagagacg caaagctaag gatgctcgtg tcctcggtcg
    12541 cagtttaaat agaaaaagtg agcgtgattt taatatcaag ggttcaattt ttggatctat
    12601 aaatagtaat gttaaacgcg gaattgttat tggcttaatt gcagctttgg taattcttat
    12661 aggagtggct gttggcgtct tcgtctttat ggcgtctact tctgcaaaac tttctattga
    12721 tgatgaagct cgcgcgaagc ttgttcgagt tcaggaaaac caagaatatt atatgttgtt
    12781 tgcagcagat gttgattcgg atgaaaacga agctcctgac atgttgatgc taactcgtgt
    12841 tgaccctcta aaaatgtcta tcataatggt gtgcatacct tcgtctactt atgttgctgc
    12901 agcacagggt ggcggaacta cgcttgaaaa tgtttataag agttctggcg atgctggatt
    12961 aatagaagca gttgcgtcat tttccgaagt tggaattacc cattatgtta aagcaagtgc
    13021 ggaaggactt tccaatttgg ttgacgcatt tggaggtatt gatgttttct taaaggaata
    13081 tgttgacgat gtaaatgttg gcgacgtcta cattggacaa ggcgatcaac atattaaagg
    13141 ctatgaaaca attactctat taaaatcaaa taattttaat ggtggaatga caaccgttat
    13201 ggataatcaa aagcaagttt gcatgggaat attaaaagca gctttattca gcaagcaagt
    13261 aaacattgct gtgctaactg atcagattgc aggttctatt aagactgata tgggagcagg
    13321 agatatcatc aattttgttg attattattc aaaaatcgaa gaagacaaaa ttatgagtac
    13381 cgatgttcca ggatataaaa caatgcgtaa caacatgatg gtatttatga ttgatgcaag
    13441 ttcgtgaaag aatcttcgta cacaaatttc ttatggaatg attttaacta ctgaagatga
    13501 tttaacttat aaagatattg atccttcaag ttttacaatc gtaattgaaa acggtgctgg
    13561 aattgatggc gcggcttcga gtcttgctga caaattaaag ggcatgggat ttaacgtaat
    13621 tgattcgcac aatgctgatt cgaacatcta taaagaaaca ttgataattt atggcgaagc
    13681 ttcgatgaaa acaaatgcca ttgctattaa aaattcgatt aataatggtc gtgtggttaa
    13741 tggcgaaggt ttgtataata tgaaaagtga tattttgatt ataatagggg ctgacctaaa
    13801 aatttaagga ggttatcatg gcacttaata aagaagatgt tcaggcagta ttagatttag
    13861 ttcgaccaag cctacaagca gatggtggtg atgtttcatt agttgatgtt gctgatgatg
    13921 gcactgttac tgttcagctc gaaggagcct gtaagggttg tccaatgtct caaatgacat
    13981 tagctaatgg tgttgagcga atcctaaaag aaagaatccc tgaagttaca aaagtagttc
    14041 cagcctaatg cgactacttc gtttctttaa aaatttctta agtgcatata tcggcattgt
    14101 ttttttgctg atcttaatat ttacgacttt tgtcggctat caattcagaa ggaattacgc
    14161 ctgtgattac atttgaattt tagttgcagt tgcggtttta atttttctaa tttatttatt
    14221 tctcaacaaa gcgaagacaa gttgaccaaa gttttgaagt tttattcata gtgataactg
    14281 tttttacttt gtgttagttg ttggaagtat tttgcttctt ttaggacaaa tttttattgc
    14341 aagtggtgcg tgatttattg taggatgaga cactcgagat gtaatttttc ctgataagca
    14401 aatgaattca gattatatgt ctatgtatcc taatcagttg tttttggctg gtttatttag
    14461 gcgtatcaat gctttggttt gtggagtttt aggttttgat ttctaccaat attatttcct
    14521 tttagttatt ttgtctgtgt tgtgtgtttt tgcttcagta atttttactg gcttaatagc
    14581 gaagaaaatt acaagtccct ttgttgcaac aatcacattt ttatgtgcga tgttatgcgt
    14641 tggctttagt ccatgaatca tggttccata ttctgatact tttgcgatgt tgtttacaac
    14701 aatgactttg ttttgatatt gctatattaa gataaatcct ttgaagtgat ttttaattcc
    14761 gttcacgagc attattggtt tttcaattaa acccactgca atttttgtat tgctcgcaat
    14821 tttgtttgtt gaactttgtt tgttgatttc aaaccacaaa gaaattatta agaaatacag
    14881 caaagtaaac ttagtaaaag tcatgatttg tattttggtt gccttgtttg gcttcggctt
    14941 tgcaagtgtt gcttcgggaa aaatttctaa ttatggtgtc caaattgata aggaccgaag
    15001 ctttaccgca actcatttct tgatgatggg aataaatctt gaaaatcatg gtggttgaaa
    15061 taatgatgat gtaatgattt cgcgcagtta cccaaatgtt gccgagcgcg aagcgggaaa
    15121 cattcgtgag tggcaacata gattgtttac ctatggaatt ccggatattt cttttatcct
    15181 gctgaacaaa acgcttacta attttaatga cggaagtttt gcttgagagg ccgagattcc
    15241 atgaatgatg gaaaacagag gtgacaatcc aattgtgtga gcattttacg gtattgatcc
    15301 tgccgatgcg cataatgaca acaacaattt ttctgcgtat ccaagacaag ctttgtgata
    15361 catgattttg ataggttgca tttttacatt cttaaataaa aagccgaaac gcgaagaatt
    15421 tgtcgcttac ctttcaattt tggcaattag tgcatttttg acagtttgcg aacctgaccc
    15481 aagatatctt ttgctttatc ttccatattt tgttctaatt gctccaaaag gatgagcaat
    15541 ggcgttcaat aagtttattc gcggtgaaca aattcaagaa gtaactaaga agaacgaggc
    15601 gaagtatggc agctactaat aataagttaa aacttggcgc atgcgtaatg attttgattg
    15661 gcggaatgtt tggaagtgca attttctcgc tttctgggct cacaattgca aacgccggcc
    15721 ctgcttcgtt aattagttga tttgcagctg ctcctataat gcttctatat ggacttttga
    15781 tggctgaaat ggcttgttat tatccaaaaa gcggcggtgt atatgtattt ccagctaagg
    15841 cattttgcgg caagaaggga aaatttttag gctggttatc ttgttggtcc tacattattg
    15901 gaaactttgc agctatcgca ttttcggcaa tttatgttgg agtttatttg ggtgctgtat
    15961 ttccagaaat ggcaaatttt caaacgccga tagctatagc ttcgcttcta tttgttttag
    16021 ttctaaatat tattaaagta aaaaggactg gacagattaa taacataatg gttgcagctt
    16081 taattatttt gatggttgtt tttgtaggcg ttgcaattac aaatagttcg tttaacgtaa
    16141 agaattttgt tccatttttc ggtcagggac aggctggaac attcggcttt gtttcaatgg
    16201 ttcctattgc acttatgtct tatagcgcaa ttgttgcagt tgcttttatg gccaacgaag
    16261 tcgacaatcc gaaaaagaca attcctcgcg catcttttat atctataata gttttatcga
    16321 ttttatactg cctaattttg tttgcaactc tcgggattgt aacggctgat tatctttcac
    16381 aaaatcctga tcttgaaatg attccgttat ttgccgcttg cgcaaaaatg attgacagac
    16441 cttgattgac ttacatcatc agttttgctt cggtaattgc tttaattaca acaatccttg
    16501 tagttgtttc tcttaatgct tgagccattc aagcatccgc cgaagacgga gtcctgcctc
    16561 gagcattttc acacaagagt aaatatgggc aaccacttat cggcctaatt attacggtta
    16621 ttatctcttc catcatttgt ttgttcccaa attttgttaa cgagatagtc aacctcggcg
    16681 ttttatttaa cattatcact atatttattg tagtaattgc cttgatgttt gcgcgcaaac
    16741 gcgaaaagtt gccaaagaac gcatttcgcg taaagggtgg tgccttctgg ccaatcctct
    16801 tcttaatagt tatagttcta tgcaatatct caaacatcct cacatcaaac ctttatttaa
    16861 tcctaattta cacaggagtc ctattactta tcggaatcat catttattga ataacacgta
    16921 agaatcatat ataatacctg gtttaatgaa taggaaagat gacataattg ttgttcaagg
    16981 caatcaaact ttgactggaa aggttgcggt ttcgggcgct aagaattcgg cgcttaaatt
    17041 gattgctgct agtattttgg taaatgatgt ttgcacaata aaaaacgtgc caaatatttc
    17101 tgacattgac attatgtgtc aggttcttga gagtcttggt gcaaaggttg atagaaaaga
    17161 ccacaccttg gttattgatt cttcgtctat taatagtgtt gaggcatctt ataagctggt
    17221 ttctcaaatg cgtgcaagca ttgctgtttt aggccctttg gtttccagat tcggcgaagc
    17281 caaggttgcc atgcctggag gatgccaaat tggtgcacgt aaaattgaca tgcacttgat
    17341 ggggcttcgt cagcttggtg tcaaattcga cgttaatttt ggctacttaa atgcaactgt
    17401 tccaagtaaa cttcgaggtg caaatattcg acttgatttt gcaagtgtag gtgctactga
    17461 aaatattttg atggcttcgg tgtgtgctga tggcagaacg attattgata acgctgctcg
    17521 cgagcctgaa attgttgatt tgtgtaactt cctaaatgaa attggatgtt caattcatgg
    17581 ggctggaaca agtgttattg aaattgaggg agtagatgtc aattcacttc atggttgtac
    17641 ttataaaact tgtggagacc gcattgaagc aggtacttac attgttggtg gtgctgctac
    17701 tggtggtcct ctaactgtcg aaggcataga cccagactat ttaaaaactg ccattagaaa
    17761 attgcgtgat atgggtgttg atgtcaaaat tgataaagat tcaattacgg tttcacgcga
    17821 tgtgactaaa cctcttagaa cggtgcaggt ccaaacactt ccacatcctg gctttccaac
    17881 cgacctgcag gcccaattta tgcttttggc ctgcttagca gatggaacgt cgataattac
    17941 agaaaatgtt tttgaaaacc gctttatgtt tgctgcagaa attatgagaa tgggagcaaa
    18001 tattagcttg caagagcatc atgctgttgt taaagggcct gttaaattac aaggtgctcc
    18061 tgtgtcttca actgacctta gagctggtgc ttcgttagtt ttagcgggca ttatagccga
    18121 aggaacaaca actgttacag aggttggaca tattgatagg ggctatgaag acttcgttga
    18181 taaattatgc cgtttaggtg cggaaataaa gcgaaaaaat cctaaaaaat agccaaattc
    18241 ttggtttaat cttaaaaatt tttgttataa tctaaatgtt gcgtggaaac gtaatttttg
    18301 caggtagtaa cctacaaaat attgtgtttt tgagcaattt taagggccaa aaagtagcat
    18361 taaaggagga aaattggctg atcaagaatc aaagcattat actggcgaag acatccaagt
    18421 tttagagggt ttagaggccg ttagaaagcg tccaggcatg tatattgggt ctactgggcc
    18481 cagaggtctt catcaccttg tatatgaggt tgtagacaac gctgtggacg aagctttagc
    18541 gggttattgc gagaacatcg atgtaacaat tcataaagat aactctatta ctgttgttga
    18601 tgatggacgt ggtatcccgg tcgacatcca tccgaaggaa aaaatcccaa ctgttgaggt
    18661 tgttcttaca attttgcatg ctggtggtaa gttcggaggc gaaggctata aggtttctgg
    18721 tggtcttcat ggtgtaggtg tttctgttgt aaacgcgctt tcaagtaagc ttgttgctga
    18781 agtttcgcgc gatggcaaga aatacactat ggaatgcgag cgtggtaaaa ctacccaaaa
    18841 acttaaagag gctggtaagt ctgataaatc aggaacagtt atttctttct ggccagatcc
    18901 agaggtcttt actgagacta cagaatatga ttacgatacg cttgcaacac gttttcgtga
    18961 aatggcattt ttgaacaagg gcttaaaaat tcatttgaag gacgagcgaa gcgataaaga
    19021 aaattcattt tgctatgacg gtggaattat tgattatgtc gagtttttga ctaaaggtaa
    19081 agaaactcta aacaagccaa tttatttcac tgctcaaaat gaagatggcg atgttgaaat
    19141 tgcgatgcaa tgaacaacaa gtttttcggc aaacagtgtc atggccttcg ctaacaatat
    19201 taatactcac gaaggtggaa cgcatcttga tggtttcaag caggctctta ctcgtgttgt
    19261 taataactat gctagaagca agggtatttt aaaggagaag gatagtaatc tttcaggaga
    19321 tgattgtcgt gaaggtttaa gtgcaatcat ttctgttaaa ttacatgatc cccaattcga
    19381 agggcagaca aagacgaagc ttggcaacac tgaaattcgt cctcttgttc aaaatgctgt
    19441 tgcagataag cttgcaagtt acttggaaga gaacccaggc cctgctaaaa agattgtctc
    19501 aaaggcatct caagcactga aggctcgcga agctgcacgt aaagctcgcg aagcaacacg
    19561 tcgcaagggt gtgcttgatt catttagttt gcctggaaag ttggcagatt gttcaagcaa
    19621 ggaagctgag aaaagtga
//
LOCUS       DBNCDC010000007        19212 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_751_length_19212_cov_7.212037, whole genome shotgun sequence.
ACCESSION   DBNCDC010000007 DBNCDC010000000
VERSION     DBNCDC010000007.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 19212)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19212)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19212
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_751_length_19212_cov_7.212037"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(537..896)
                     /locus_tag="AC04VQ_01185"
     CDS             complement(537..896)
                     /locus_tag="AC04VQ_01185"
                     /inference="COORDINATES: protein motif:HMM:NF039324.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6020 family protein"
                     /protein_id="HIO9552861.1"
                     /translation="MISIVVLIILAIKFKSMRKIWLASLLSVFVIFTSFILIKSIGII
                     QSAHFSETVAIPLQQIAYTAKSNDGLDEEQDFFENIISVEEMKKLYVENSADEVKFSP
                     NFNDDFLDTHKLEFIIN"
     gene            complement(1050..1400)
                     /locus_tag="AC04VQ_01190"
     CDS             complement(1050..1400)
                     /locus_tag="AC04VQ_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552862.1"
                     /translation="MCFIPGQADNDSEDVFKMILGLDFYSDTFRYTQLNDHNPLIYTF
                     FNWFVFSIAKIFCADNLILVSTVCAVQMIVVIICIVYSINKLYMFSNNLIFVVIATLF
                     FLINPLIIQYSLSH"
     gene            1555..2691
                     /locus_tag="AC04VQ_01195"
     CDS             1555..2691
                     /locus_tag="AC04VQ_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HIO9552863.1"
                     /translation="MKKFILLFCPVLFICVLIFAGCVNQNIPYLDDSQNATINSARAI
                     KNTDNKAYEINYTADYKASEFIDLITKEKMTSAYEIRTKMKDLLAPKSKVFPLDRLSN
                     SAPGCSSVICKNADGQPVIGRNYDLDVNSNGLTSVIHTSPCDGYKSVGVSDVGQCGLS
                     YNDLANQNTNKELFLYAPYFTMDGVNETGFACSIMILNEGQTVQDTGKNWLPATMVVR
                     YLLDHANSVDNAIELLNSFDYRNDYFVEPWLVDMNFHWALTDKSGNKAVVEFINGKMC
                     VNKYPVKVKYNETTDEIQIQYPKQETGYLLSTNFYVTEGFKNTVHDAGKWRYQTLDEL
                     MSKNPTPTKDELKDIMKSVKFFMNDKDFMWELRQDGKDVNKAEN"
     gene            2893..3777
                     /locus_tag="AC04VQ_01200"
                     /pseudo
     CDS             2893..3777
                     /locus_tag="AC04VQ_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
     gene            3866..4291
                     /locus_tag="AC04VQ_01205"
     CDS             3866..4291
                     /locus_tag="AC04VQ_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HIO9552864.1"
                     /translation="MHADFDSFMANYDVDCVVSPGNSHGVMAGGYDRALSEYFGWDLT
                     KRVQAYIKENYEGQQPVGSSFIIDTGMDGINLIHTPSMINPSRIQDPNVVYECTKSTL
                     EVARQNNVQSIVIPAFGGACGGLRPEVIAEKMYTAINEF"
     gene            4304..4819
                     /locus_tag="AC04VQ_01210"
     CDS             4304..4819
                     /locus_tag="AC04VQ_01210"
                     /inference="COORDINATES: protein motif:HMM:NF015325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIO9552865.1"
                     /translation="MKVVVFNGSPRKNGDTAKMTGVFKEVAEKAGHDVKIFDVANMNI
                     NGCKGCEYCHQKSDGICIQHDDMDLMYDDLREVEVLILASPIYYFTMSGQIQTALNRT
                     YCIGRFPKLKKLGMILSSHDNKAFGACKAQHDDLCRYLELDSAGIITSHQEEHGTEAK
                     LAEIEQMAQNL"
     gene            4833..5870
                     /locus_tag="AC04VQ_01215"
     CDS             4833..5870
                     /locus_tag="AC04VQ_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013383.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552866.1"
                     /translation="MSVVTLIGSGQMASALTFPLFENGHEVRLVGSPLDDEVIDRLCE
                     DNYHINLRRTLHDGINYYKNDAMKDALQGADLVVCGVSSFGVDWFADEVLPILDENVP
                     LISVTKGMLDHEDGSMEVYPNYWQSKLPKDSKLKLYAIGGPCTARDLSDHDPSFVAYC
                     GPDFETLEKIRDMFSTDYYIISLTQDVVGLEAAVALKNGYALGTALAIGMAEKAGDDG
                     IDHNNSESALFQQSVKEMLDLLRIVGGKDENIMFAAGDLNVTVAAGRSRTVGLMLGQG
                     YTIEETLKKLEGQTLEAVVIATRTARAVKALAKRGKVNLDDFPLLMHVDELLNEGKTV
                     NIPWKKFEKKM"
     gene            5886..6614
                     /locus_tag="AC04VQ_01220"
                     /pseudo
     CDS             5886..6614
                     /locus_tag="AC04VQ_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012589.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
     gene            6654..7391
                     /locus_tag="AC04VQ_01225"
     CDS             6654..7391
                     /locus_tag="AC04VQ_01225"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIO9552867.1"
                     /translation="MTTIGTSGTVFAFTEKPVTDPGQAVYTYCMPVPGAWHFMGSVNS
                     CGDSLKWWRNEMYPNDAEYEEINKVAQASQPGANRLIYLPYLNGEQSPHFNLNCRGSF
                     VGLSKVHTKGDMTRAVMEGATYALRDILTAIRNADIEPDNVIMCGGGSKSPFWRQLMA
                     DIYNIPVKLPDMNSENAAALGVAILAMVGTGAYPSVVEACDKIIKMRDEVYTPDVERA
                     KFYDKVYAEFDKLYPQLKDSFKSILEL"
     gene            7401..7838
                     /locus_tag="AC04VQ_01230"
     CDS             7401..7838
                     /locus_tag="AC04VQ_01230"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HIO9552868.1"
                     /translation="MKIVFGCDPNAEDFKQQLIEFTKSLGHDVEDFGDTDPIYANTAI
                     KVAKAVAAGDFDKGILLCGTGIGVSIAANKVKGAYAANIHDVYQAQRAQLSNNANIIT
                     MGSQVIGIELAKQLVKEFLGAGEFDMNGRSVAKVQRIVDFENE"
     gene            7848..8342
                     /locus_tag="AC04VQ_01235"
     CDS             7848..8342
                     /locus_tag="AC04VQ_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HIO9552869.1"
                     /translation="MAKHLMGIDCGTGSVRVAIFDETGNNLAYAISEYGTTYPKNGWA
                     EQSDVEWLDALKTAIPESIKKAGVDKNDIAAICCDATTNTLVYLGKDDKSVRPPILWM
                     DVRAAEEADFIDSIRKDYEATRFYKPAFRADTMIPKNMWVKKYEPENWASTQTMFEFE
                     DWLN"
     gene            8625..9347
                     /locus_tag="AC04VQ_01240"
                     /pseudo
     CDS             8625..9347
                     /locus_tag="AC04VQ_01240"
                     /inference="COORDINATES: protein motif:HMM:NF014801.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
     gene            9446..10864
                     /locus_tag="AC04VQ_01245"
     CDS             9446..10864
                     /locus_tag="AC04VQ_01245"
                     /inference="COORDINATES: protein motif:HMM:NF022525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HIO9552870.1"
                     /translation="MAWLKKYKPEVEVIPDNLEARFEAGTEVGELAKGLFGDYVDVTV
                     RDGDSLDIKAMLEKTQEELENKPNVICEAAFSFNGLYCACDLLKNDGDGWSIYEVKSS
                     TDGEKDVYIQDLSYQRYVLENCGVKINKTFTVVVNNEYVLEDELDIQEFFKITDMTEA
                     VLAEMENVPQNLEEAEKILAMKDEPDIDLSNNCHDPYDCAFWDYCSRHLPTPSVFNLY
                     RLGFKKKLDFYNRGMVSFEELKNFPEEFNEKQNRQIQYALNDFGLYADPECIKKFLET
                     LSYPLYFLDFETMQLVIPRYKGTHPYQQITFQYSLHYIEEEGGELKHKEFLAESGPDP
                     RRALAEQLCKDIPLNVCTTAYNRGFECGRIKELAEAYPDLADHLLNIRDHIEDLLVPF
                     SKGWMYNKEMGGSFSIKSVLPALFPDDPELDYHNLDQVHHGGEAMAIFPAIEHMNDED
                     KKRARHNLLKYCELDTYAMVKV"
     gene            complement(10884..11879)
                     /locus_tag="AC04VQ_01250"
     CDS             complement(10884..11879)
                     /locus_tag="AC04VQ_01250"
                     /inference="COORDINATES: protein motif:HMM:NF023190.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase family protein"
                     /protein_id="HIO9552871.1"
                     /translation="MRVCVGVAFSKNLKVMRFDDNMRNVAVTEGDKVGVQEVLGWQVN
                     TWPVGQLVEYIEDVSEDEVDEKIKEYKKAYKFNTKNMDAVRYQAKEEIAIKRMLDEEG
                     CMAFTNTFEDLYGMEQLPGLASQRLMEQGYGYGGEGDWKVSAMTAIIKAMSEGQSGGS
                     SFMEDYTYELDEKGQFSLGAHMLEVCPSIASKQAQIEVHPLGIGGKSDPARLVFEGRA
                     GKAIVVSLVDMGGRLRMIVQDIEAVKPIYSMPNLPVARVMWKAMPDLATGVECWIMAG
                     GAHHTTLTYDVSAEQMHDFARIMGIEFVHIDKDTTPEKLEKELFCNDLAYKLNAN"
     gene            complement(11880..12368)
                     /locus_tag="AC04VQ_01255"
     CDS             complement(11880..12368)
                     /locus_tag="AC04VQ_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552872.1"
                     /translation="MKKMNDYKFWFVVGSQFLYGPEVLETVASRAQEMADTLNKSGKL
                     PCEIVYKVTAKTPEEITNVVKEANYDDSCAGIITWMHTFSPSKMWLNGLKLLQKPYCH
                     LATQYNKSIPNKEINMDFMNLNQAAHGDREHGFIAARLRIPRKIIAGYWKDAKVQKRL
                     GD"
     gene            complement(12399..13841)
                     /locus_tag="AC04VQ_01260"
                     /pseudo
     CDS             complement(12399..13841)
                     /locus_tag="AC04VQ_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(13922..14095)
                     /locus_tag="AC04VQ_01265"
     CDS             complement(13922..14095)
                     /locus_tag="AC04VQ_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552873.1"
                     /translation="MGEEMCAYLDGFGTKTFVYELQGIIYVVNGVPVQTKDGYIIKSY
                     APFPKEFHFLDKD"
     gene            complement(14099..14332)
                     /locus_tag="AC04VQ_01270"
     CDS             complement(14099..14332)
                     /locus_tag="AC04VQ_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552874.1"
                     /translation="MLDAAYYATVAILYCYDTHANACNLRLNKGEAASTYSVAGPLIN
                     FPKISRDRFGQSVFAELGGIFSKLFANDDSTMD"
     assembly_gap    14474..14573
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14772..15125)
                     /locus_tag="AC04VQ_01275"
     CDS             complement(14772..15125)
                     /locus_tag="AC04VQ_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552875.1"
                     /translation="MPHKYYEPTGGFIYFQVHEQGEHKIEVVMDDGCSDCVVLNVMDK
                     IPQLLKERCDYICDTLYHGKNDKVPYCFYPVSNQGESIGKLNLVLKYSILANKFNPSH
                     IKKVEESAVYYIKQK"
     gene            complement(15519..15785)
                     /locus_tag="AC04VQ_01280"
     CDS             complement(15519..15785)
                     /locus_tag="AC04VQ_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552876.1"
                     /translation="MEVIYNLRDAEYEGLDFKVQLTNKTNENFQINDFRVWTPFAHIM
                     YRDKNVLKNINESCTVTPSISKDFTRLAMVRNNNKGPNLGMYQT"
     gene            complement(16273..17115)
                     /locus_tag="AC04VQ_01285"
     CDS             complement(16273..17115)
                     /locus_tag="AC04VQ_01285"
                     /inference="COORDINATES: protein motif:HMM:NF014801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIO9552877.1"
                     /translation="MFPINLETHNYDEEMLNKFSDLIKDKNFDWNLKDILPKVLVAGE
                     NAGTLTDEGAKLLDKSGKLQGGAIACPPEGDAGTGMVATNSLAKRTGNISAGTSAFAM
                     LVLEKNLKELHTTIDMVTTPAGDLVAMSHANNCTTEINAWVSLFKEVLELGGSNVDTN
                     ELYTLLFENSLKGDADCGELLPYCFHSGEDIVGLEQGCPSFIHPTKANFNLANFIRAQ
                     IYSCFAAMKIGMDILIKDEGAKIDKMVCHGGIFKTEGVAQRFLAAALNTNIETFSTAS
                     EGGA"
     gene            complement(17326..17457)
                     /locus_tag="AC04VQ_01290"
     CDS             complement(17326..17457)
                     /locus_tag="AC04VQ_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552878.1"
                     /translation="MCKDVKEKYAAEITKLKAFGISGMMHGYLPFDKQGNQLAEFRT"
     gene            complement(17509..17670)
                     /locus_tag="AC04VQ_01295"
     CDS             complement(17509..17670)
                     /locus_tag="AC04VQ_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552879.1"
                     /translation="MDNEKNKTIIENGDGILGVEFGSTRIKAVLIDEAGEVLATGGHN
                     WENHFENNL"
     gene            complement(17672..18889)
                     /locus_tag="AC04VQ_01300"
                     /pseudo
     CDS             complement(17672..18889)
                     /locus_tag="AC04VQ_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012312.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
     gene            complement(19067..19140)
                     /locus_tag="AC04VQ_01305"
     tRNA            complement(19067..19140)
                     /locus_tag="AC04VQ_01305"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19106..19108),aa:Cys,seq:gca)
BASE COUNT         5819 a         3601 c         3851 g         5841 t
ORIGIN      
        1 ttttaacatg cagataatta taaactataa cgtaacgtaa tagtcaagtg taattttgaa
       61 ttagtttcct aaaccgtgcg tcggaggttc gaactccgag cacaacgcca gctctttagg
      121 attatcaagg cgattagggg aagacataaa tgcagtgggt atacgtatct aaaatcgctt
      181 gctggtgttg caaccatcaa ggataatcaa agcaataaca atgggcttaa agcaattaga
      241 tagttaggtt tttttctagc tatgcagata atggctgcta ctaatacgat atataaaagt
      301 atagatggat tatatatagg gttaatctga tttaagagtg aatccattga gcttttaagg
      361 tcattgcaat cgacaatatt ttccaggatg tttttctgaa ggtattttcc attctctaaa
      421 ctaagaccta tataatccaa agtgaatcat gtttctgcag cagggtttca aaatgcttcg
      481 gtctgtttag ctcatgctgt tgcatattcg ggtatatgtt tagagccaag ctttgctcaa
      541 tttatgataa attccagttt atgcgtatcg aggaaatcgt cgttaaaatt tggcgaaaac
      601 tttacttcgt cagcagagtt ttctacatat aatttcttca tttcttcgac actgatgata
      661 ttttcaaaaa aatcttgctc ttcatctaaa ccatcattgc tttttgctgt ataggcaatt
      721 tgttgaagag gtattgctac ggtttcggag aaatgggcac tttgtattat gccgattgat
      781 ttaattagta taaaagatgt aaatataaca aacacgctaa gtagcgaggc tagccaaatt
      841 tttctcattg atttaaattt tatagccaag atgataagca ctacgatgct gatgatgaat
      901 ccattttttc ttcaaaaagc acaaagtata agcatcgata ttagtaatat tagattggat
      961 ttattctttt tgaaatcatc aaaatgagca atcatatgaa ataagttaat gcacagaaca
     1021 agaattatgc atccaaaaat tgcatccttt caatgtgata gggagtattg aatgattagt
     1081 ggattaatta agaaaaataa ggttgcaata acaacaaata ttaaattatt agaaaacata
     1141 tatagtttat ttattgaata cacaatacaa attattacaa caatcatttg tactgcacaa
     1201 acagtagaca ctaatattaa attgtcagca caaaatattt ttgcaatact aaatacgaac
     1261 caattaaaaa atgtataaat tagcgggttg tggtcattta gttgagtgta cctaaacgta
     1321 tcagaataaa aatccaaacc tagaatcatt ttgaagacat cttcggagtc attatcagct
     1381 tgcccaggaa taaagcacaa aaagaaaaac cctcatatga ttaaaaatgc agcaaatatt
     1441 ataaatgtat atttcttttc aagagttttc atcgattagt attataaatg tttcttatgc
     1501 tatggtaaaa tttatataaa agtatttgta cgcaaaggag gtaggaggga aattatgaaa
     1561 aaatttattc tattattttg ccctgtttta tttatttgtg ttcttatttt tgcaggttgc
     1621 gtcaaccaaa acattccgta tttagatgat tcgcaaaacg caactattaa cagtgccagg
     1681 gcaattaaaa acactgacaa caaagcatat gagattaact atacagcgga ttacaaagct
     1741 tcggagttta ttgatttgat aacgaaggaa aagatgactt cggcgtatga gattcgtaca
     1801 aagatgaaag atttattagc tccaaaatct aaggtttttc ctttagatag gctttctaat
     1861 tcggcacctg gttgtagttc tgtaatttgt aaaaatgctg atggacaacc tgtaattgga
     1921 agaaattatg atttggatgt aaactctaat gggttaactt ctgtaattca tacaagtcct
     1981 tgtgatggtt ataaatctgt tggcgttagt gatgttggtc aatgtgggct ttcttataat
     2041 gaccttgcga atcaaaatac caataaggag ctattcttgt atgcaccata tttcactatg
     2101 gacggtgtaa atgagactgg ttttgcatgc tcaattatga ttttaaatga ggggcaaact
     2161 gttcaagata ctgggaaaaa ttggcttcca gcaacaatgg ttgttagata tcttttagat
     2221 catgcaaata gtgtagacaa tgcaattgaa ttgctaaatt cttttgatta tagaaatgat
     2281 tattttgttg aaccatggtt ggtggatatg aacttccatt gggccttgac tgataagagc
     2341 ggtaataagg ctgttgtaga atttataaat ggaaaaatgt gcgttaataa atatcctgta
     2401 aaggttaaat ataatgaaac gactgacgaa attcaaattc aatacccaaa acaggaaact
     2461 ggttatttac tctcaacaaa tttttatgtt accgagggtt ttaaaaatac agttcatgat
     2521 gcaggtaagt ggagatatca aacattagat gaattaatgt caaagaaccc aactccaaca
     2581 aaagatgagc taaaagatat aatgaaaagc gttaaatttt ttatgaacga caaagatttt
     2641 atgtgggagt taaggcagga cggaaaagat gttaataagg cagaaaactg agagtgagaa
     2701 actgtttgga cagacattct aaacacagat gaccttacat taaccttcta cctaaaagaa
     2761 aactttgatg tagaaaacgt ctttacaata gattactagt tggctttgaa atgataattt
     2821 gtagttagat atttaaacaa tggtgaaaat ggggacatcg ctgaaatctt aatattttgt
     2881 tataattaat tcatggaaga taagttttta aaaaccgcaa agaaagtttc ttatatctca
     2941 atcgcagtaa atattggatt gactttatat aagtttgtgt ctggtgttct tggaaattcg
     3001 caggcccttg taagtgatgc agtgcactcg gcatcggatg tttttagctc aattattgtt
     3061 ttgattggca tacgatttgc ggctaaggaa gctgacgaat accaccctta tggctacgaa
     3121 cgtttcgaat gtgtagctgc tattttgctt tcttttttgc ttactgcaac agctgttgga
     3181 attggatatt cggccgctac aagtattatt tttggagaat atgaagctgc ggaacttccg
     3241 aattttgttg ctttagttgc cgctattatt tcaattgcag ctaaggaagg catgtattga
     3301 tatactcgct acaatgcgaa gaaaatcaat tcaacggcgc ttatggcaga tgcttggcat
     3361 caccgctcag attctttgtc ttcggttgga gctttaattg gtattgcatt ttcgatgttt
     3421 gggtttcata ttatggaccc aattgcctcg cttgtaattt ttgtgctaat tatgaaagct
     3481 gcaattgaaa tttttgttga cgcaatcaat aagatgacag atgcatcggt tgacaagaaa
     3541 gttttaaaga agtataaaga tttcattaaa aagaacccag atgtaaaatc aatttcgcaa
     3601 attaggaccc gaaagtttgg tccacgagtt tttgttgatc ttgaaattgc cttagataaa
     3661 agtctttgtc tttacgaagc cgaaggtatc gccaaaacag tacatgattc aatggagaag
     3721 cgtttcccag aaatcaaaca cataatggtg cacgttagcg ccgaaaaatc taaataattt
     3781 tatgaagatt tatttgttag acataaatcc ggctgttgtg aaagcttgaa acgagaagtt
     3841 ttcaggtgtt gatggcgtcg aagttgtgca tgcggacttc gatagtttta tggcaaacta
     3901 tgatgttgat tgtgttgttt caccaggcaa ttcgcatggt gtgatggcag gcggttatga
     3961 tcgcgcgcta tcggaatact tcggctggga tttaaccaaa cgcgttcaag cctatattaa
     4021 agaaaattac gaaggccagc agccagttgg atccagcttt ataattgaca ctggcatgga
     4081 tggcataaac ctaattcaca cgccttcgat gattaatcct tctcgcattc aagatccaaa
     4141 tgttgtttac gaatgcacga aatcgacttt ggaagttgct cgccagaaca atgttcaaag
     4201 cattgtgatc ccggccttcg gaggcgcgtg tggaggactt cgtccagaag ttatcgccga
     4261 aaaaatgtat actgcaatta acgaatttta gaaagggaag aaaatgaaag ttgttgtttt
     4321 taatggtagt ccaagaaaaa atggtgatac tgctaagatg actggtgttt ttaaggaagt
     4381 agcagaaaaa gcaggtcatg atgtcaaaat ttttgatgta gcaaacatga atataaacgg
     4441 ttgtaagggc tgtgagtatt gtcatcagaa aagtgatggt atttgcattc agcacgacga
     4501 tatggattta atgtatgatg atttgcgcga agttgaagtt ctaatccttg cttcgccaat
     4561 ttattacttt acaatgagcg gtcaaattca aacagctctt aatagaacat attgtattgg
     4621 aagattccct aaactaaaaa aacttggaat gattttgtcc tctcatgata ataaagcttt
     4681 tggtgcgtgt aaagcacaac acgatgactt gtgcagatat ctagagttag atagtgccgg
     4741 cattataact tcgcatcaag aagaacacgg cactgaagcc aaacttgctg aaatcgaaca
     4801 aatggctcaa aatttataat taaggggcag gaatgagtgt tgttacgtta ataggttctg
     4861 ggcaaatggc ttctgcctta acttttccgc tgttcgaaaa tggacatgaa gttaggttgg
     4921 ttggcagccc tttggatgac gaagttattg atcgcttgtg cgaggataat tatcacatta
     4981 atttgcgtag aacgttgcat gatggtatta attactataa aaatgacgct atgaaagacg
     5041 cacttcaagg tgcagattta gttgtgtgtg gagtatcgag cttcggcgtt gattggtttg
     5101 ctgacgaagt acttccaatc ctggatgaaa atgtcccgct gatttctgta actaaaggca
     5161 tgcttgatca cgaagatgga agcatggaag tttatccaaa ttattggcaa tccaaacttc
     5221 caaaagactc taagctaaag ctttatgcaa ttggtggtcc ttgcactgct cgtgatttgt
     5281 cagaccatga cccttcgttt gttgcatatt gtggacctga ttttgaaact cttgagaaaa
     5341 ttcgcgatat gttttcgact gactattaca ttattagttt gactcaagat gttgttgggc
     5401 tcgaagccgc tgttgctctt aaaaatggtt atgctttggg aactgctctt gcaattggta
     5461 tggctgaaaa ggctggcgat gatggtatcg accacaacaa ttcggagagc gctctatttc
     5521 aacaaagtgt aaaagagatg ttggatttgc ttcgtattgt tggtggtaaa gatgaaaata
     5581 ttatgtttgc ggcaggcgac ttaaacgtaa ctgttgctgc gggacgaagc agaactgtag
     5641 gcctcatgtt ggggcagggt tatacaattg aggaaacgct taagaagttg gaaggccaaa
     5701 ccttagaggc cgttgtaatt gcgactcgca ctgctcgcgc tgttaaggca cttgcgaagc
     5761 gaggtaaagt taacttggat gattttcctt tgcttatgca tgttgatgaa ctgctaaacg
     5821 aaggaaaaac tgtaaatatt ccgtggaaga aattcgaaaa gaaaatgtaa tttggaggtg
     5881 taattatgaa ttattatttg ggtttagact taggaacggg aagcatgaaa actgtgcttt
     5941 tcgacgtcaa tggaaaagag attgctcaaa atgcgatcga atatccaatt tatcaaccta
     6001 ataatggctg gtcagagcag gaacctgagg attggtatca agctgctgtt aaaactgttc
     6061 gcacagtaat ggacgaggcg aaggttgacc cggcttcggt gcttggtctt ggaatttctg
     6121 gtcaaatggc tggggctgtc ttcgctgata aaaacggcga agttttgcgt cgtgcaattt
     6181 tatggaatga tgcgcgaaca actgaggctt gcgaacacct ccgcgaccga gttggaaatg
     6241 acaccttcct aaaacatgtt tgcaatccgg ctcgtcctgg tcttcaggct tcgcgaattc
     6301 agtgaatgca agaaaatgag cctgacattt atgctcaagt tgcacatatt atgcttccaa
     6361 aggattattt gcgatatcgc ttaactggtg aatatgccac cgaagtttcg gacgcatctg
     6421 gaactcaaat tttggatgtt ccaaaacgta aatggtgcgg tgaaattatg gaaattatgg
     6481 gacttaccga agatatgatg ggtaaggttt atgagtccgc cgaaattaca ggttatgttt
     6541 tagaagacgt tgcgcgcgaa atgggtatta cttcgaaatg tgctgttgtt ggaggcgcag
     6601 gggataatgc tgccgctggt gttggaactg gaattgttgc tcctggtcgt gcaatgacaa
     6661 ctattggcac aagtggaaca gttttcgcct tcaccgaaaa gcctgttaca gaccctggtc
     6721 aagcagtcta cacttattgt atgcctgttc ctggcgcatg gcattttatg ggaagcgtaa
     6781 attcttgtgg tgattctctc aagtggtggc gcaatgaaat gtatccaaat gatgctgaat
     6841 acgaagagat taataaggtt gcccaagctt cgcaacctgg tgctaacaga ctaatttatc
     6901 ttccatattt aaatggtgaa caatctcccc acttcaattt gaactgccga ggttccttcg
     6961 ttggtctttc gaaggttcac acaaaagggg acatgactcg tgcagttatg gagggcgcaa
     7021 cttatgctct tcgtgatatt ttgactgcaa ttcgcaatgc agatattgag cctgacaacg
     7081 ttattatgtg cggtggcggt tcgaagagtc ctttctggcg tcaacttatg gctgatattt
     7141 ataacattcc agtcaagctt ccagacatga attctgaaaa tgccgcggct cttggcgttg
     7201 caattctagc catggttgga actggcgctt atccaagtgt tgtggaggct tgcgataaaa
     7261 tcattaagat gcgtgacgaa gtttacacgc cggatgttga gcgtgccaaa ttctatgaca
     7321 aggtttatgc cgaatttgac aagctctatc cacaacttaa agatagcttt aaatctattt
     7381 tggaacttta ggaggaattg atgaagattg tttttggttg cgacccgaac gccgaagatt
     7441 tcaagcaaca acttattgag ttcactaaaa gtttaggaca cgatgtggag gacttcggag
     7501 acaccgaccc aatttatgct aacactgcaa taaaagttgc caaggcagtt gccgccggag
     7561 attttgataa aggaatcttg ctttgtggga caggaattgg tgtttcaatt gctgcaaaca
     7621 aggttaaagg cgcttacgct gcaaatattc acgatgttta ccaggcacaa cgtgcgcagc
     7681 tttcgaacaa cgcaaacatc ataacaatgg gctcgcaggt aattggaatt gagcttgcta
     7741 agcaacttgt taaagaattt ttgggtgctg gcgaatttga tatgaacggc cgaagtgttg
     7801 cgaaggttca gcgcatcgtt gactttgaaa atgaataggg ggaaattatg gctaagcatt
     7861 taatgggaat tgattgcggc acgggaagtg ttcgtgttgc aatttttgat gagactggaa
     7921 acaatcttgc ttatgcaatt tctgaatatg gcacaactta tcctaaaaat gggtgggctg
     7981 aacaaagtga tgttgaatgg ctcgatgcgc taaagaccgc gattcctgag agcatcaaga
     8041 aagctggcgt tgacaaaaac gacatcgctg caatttgttg cgatgctaca accaacactt
     8101 tagtttactt aggcaaggat gacaaatctg tccgtcctcc aattttatgg atggatgttc
     8161 gtgctgccga agaggctgac ttcattgatt cgattcgcaa agactacgaa gccactcgct
     8221 tttataagcc tgcatttagg gctgatacta tgatcccaaa aaatatgtgg gttaagaaat
     8281 atgagcctga gaactgggca agcacccaga ctatgtttga attcgaagac tggcttaatt
     8341 gagttttgac tggaaaaaag actttaagta aatctgttgc tgcgtttcgc tggaacttcg
     8401 atgataaaaa tggcggcctt ccaacggatc tttttgaggc ttgtggaatt ggcgacgttg
     8461 ttgacaaaat tccaaccgaa gtcttaagcg ttggcgatgt tgtaggtaac gtaagtgagg
     8521 ctgcggctga gatgtttggc cttagcacaa atactgttgt tgtcgaaggc accgcagatt
     8581 gcaacgcttg tatgtttggg gtcgggggtg tgcgtccaaa tggcatgact ttaattggtg
     8641 gcaccagcac ttgtttgctc ggtctttccg agaaggactt ccatgttgat ggagttaatg
     8701 gcacctatcc aaattgcatg tatgagggca caagtttgct cgaaggcggg cagaccgctt
     8761 cgggggcaat tcttacttgg tttagaaaca atttgattcc agcttcttgg accgaagaag
     8821 ccgtgtctca cgatatgaac atttatgact acattacaaa gcaagccgaa agcattccaa
     8881 ttggcgccga tggtcttgtc atgatggatt atttccaggg caaccgtgct ccttacgctg
     8941 attctaaagc tcgtggtatg ttttgaggtc tttcaattgg cacaagtcca gcgcatattg
     9001 ctcgtgcggt ttacgaaggc gtagcctacg gtgcaaatca ttgccttctt agcatgaaag
     9061 acgccggcta tgaaattaac gaaattatcg catgcggagg aattgctcaa tctccattct
     9121 ggatgcaaat gcattgcgat gtcacgggaa tcccaatgta cacgaccgtt gaaagccaga
     9181 gcgcgggttg cttgggtgat gccatcatcg ctgcaaaagg tataggaatc tataaagatt
     9241 tcgccgaagc cgcagacgcc atggttcgca aagataccga atacaagcca gacatgaaag
     9301 ctcacgaaga atacaaattc tacatggatc gctacatgga aacctgacca caaatgcgcg
     9361 acatcgtcca caaaaccgtc gatcatggag ttaaataatg catttttcaa agtctaaata
     9421 ctgtgggttt tgccagtgtc ctaagttggc atggcttaag aagtataagc ccgaagttga
     9481 ggtaattcct gataatttag aggcacgttt tgaagctgga acggaagttg gggaattggc
     9541 gaaagggctc ttcggtgatt atgttgatgt tactgttcgc gatggagaca gccttgacat
     9601 taaagcgatg cttgagaaaa ctcaggagga gctggaaaat aaacctaatg taatttgcga
     9661 agcagcattt agcttcaatg ggctttattg cgcttgcgat ttgctcaaaa atgatggtga
     9721 tggctggagc atttatgagg ttaagagctc tacggatggc gaaaaggacg tctacatcca
     9781 ggatttgtct tatcagcgtt atgttcttga gaactgcgga gtgaaaatta acaaaacatt
     9841 tactgttgtt gtaaataacg aatatgtctt agaagacgag cttgacattc aagaattttt
     9901 caagattaca gatatgaccg aagccgttct tgcggagatg gaaaatgttc ctcaaaattt
     9961 agaggaagcc gagaaaatat tggcaatgaa ggatgagccg gatattgatc tttcaaataa
    10021 ttgtcatgat ccttacgatt gcgctttttg ggattattgc tcgaggcatt tgccaacgcc
    10081 ttcggtgttt aatttataca ggttaggatt taaaaagaaa cttgattttt ataacagggg
    10141 aatggtgagc ttcgaagagc ttaagaattt tcctgaggaa tttaacgaaa agcaaaacag
    10201 acaaattcag tatgccttga atgattttgg attgtatgca gaccctgaat gcattaaaaa
    10261 gtttttagaa accttaagtt accctttgta ttttcttgat tttgagacta tgcagctggt
    10321 aattcctcgt tataaaggca cacatcctta tcaacaaatt acatttcagt attcgcttca
    10381 ctatattgaa gaggaaggcg gggaactgaa gcataaggaa tttctggctg agtctggacc
    10441 tgatcctcgt cgtgctttgg cagaacagct ttgcaaagat attccattaa acgtgtgcac
    10501 gacagcttac aacagaggtt ttgagtgtgg cagaattaaa gagttggctg aagcttaccc
    10561 agatttggca gaccatttgc ttaatattag agaccatatt gaagatttgc ttgtgccatt
    10621 tagcaaggga tggatgtata acaaagagat gggtggctcg ttctcaatca aaagtgtttt
    10681 gccagccttg ttcccagatg atccagaact tgattatcac aacctcgacc aagttcatca
    10741 cggcggcgaa gccatggcaa tctttccagc catcgaacac atgaacgacg aagataaaaa
    10801 acgagcccgc cataatcttt tgaaatattg cgagctcgat acatacgcta tggtaaaagt
    10861 gtgagaagca cttcgcgaag cttctaatta gcgtttaatt tgtaagctaa atcgttgcag
    10921 aataattctt tttctaattt ttctggtgta gtgtctttgt caatgtgtac aaattcaatg
    10981 cccatgattc ttgcgaagtc atgcatttgc tctgcactta catcgtaagt taaggttgta
    11041 tgatgggctc caccagccat tatccagcat tcaacaccgg tagcaagatc aggcattgcc
    11101 ttccacatta ctcttgcaac aggaaggttt ggcattgaat aaattggttt aacagcttcg
    11161 atatcttgaa caatcatacg aagtctgcca cccatgtcta ccaaagaaac aacaatagcc
    11221 ttgcctgcgc gtccttcaaa aactaagcga gcaggatcac tcttgccacc aatacctagc
    11281 ggatgaactt caatttgagc ttgcttcgaa gcgatgcttg ggcaaacttc caacatatga
    11341 gcgccaagag agaattgacc cttttcatca agctcataag tgtagtcttc cataaacgaa
    11401 gaaccgccgc tttgtccttc agacattgct ttgatgatag ctgtcatggc agaaactttc
    11461 cagtctcctt cgccaccata accataacct tgctccatta aacgctgaga agcaaggcca
    11521 ggaagttgtt ccatgccgta gaggtcttcg aaagtgtttg taaatgccat gcagccttct
    11581 tcatcaagca tgcgcttaat agcaatttct tctttagctt gatatctaac tgcatccatg
    11641 tttttcgtat tgaatttgta agcctttttg tattctttaa ttttttcatc gacttcatct
    11701 tcgctaacat cttcgatata ttcaacaagt tgacccactg gccatgtatt aacttgccaa
    11761 cccaaaactt cttgaacgcc aactttgtca ccttcggtaa ctgcaacatt tctcatgttg
    11821 tcatcaaagc gcataacctt caggttctta gagaatgcaa caccaacaca aactcgcatt
    11881 caatcaccaa ggcgtttttg aaccttcgca tctttccaat aaccagcaat tattttacga
    11941 ggaattcgaa gccttgcagc aataaaacca tgctcacggt ctccatgcgc tgcctggtta
    12001 agattcataa agtccatatt aatttctttg ttaggaatgc ttttgttata ttgagttgct
    12061 aaatgacaat atggtttttg caaaagcttt agaccattaa gccacatctt agaaggggag
    12121 aatgtgtgca tccaagtgat aattccagcg caactatcat catagtttgc ttccttaaca
    12181 acatttgtaa tctcctccgg agttttggca gtaaccttat aaacaatttc gcaaggaagt
    12241 ttgccagact tatttaaggt gtctgccatt tcttgggcgc gcgaagcaac ggtttccaaa
    12301 acttcgggac cgtataaaaa ttgacttcct acaacaaacc aaaatttata atcattcatc
    12361 tttttcataa tttctccttt tactttacta agatattatt atttcttctg agcgtgacgt
    12421 tttgcaagct cttttttaat cataggctcc atcttttcaa atttaatata gaaaagcata
    12481 attacagcaa caattgcata aactattaca ggtaagtaaa tgaagcaaaa actaattgcg
    12541 tttagcgcct cgggcgtttg aacctgatta gcaacatagc ctgccgcatt cataatttca
    12601 agtggaacat aagcgccgaa gccactgcct gcctggatgc aaaatgcaga tccaacagca
    12661 gttaagaatc ctgctgcgtg aactccattt ttccattcgc cgtaatcaac cgtgtctgca
    12721 agcattgcaa acggcatacc tactgctact gatgctccca aaacaccaac cgtccagaaa
    12781 aaggtaaata caaccatgtc tcctgggatt ccaagaatgt caacacctgc aaagttagca
    12841 gcaagaggaa tgcaaagttg tccaactgca ccaacagcaa gaccaatcaa cattgttcca
    12901 gttttacttg tgtgcttaac taagaaaggc atactaaatg ttccaattaa gcctaaaact
    12961 tgaacaccat taataagtga agtcaggttt tcgtttccaa gaacatattt agcgtaataa
    13021 gttgatgtgc catttcttgt gctttgtgca acccaataaa ttacaaatgc aactaccaaa
    13081 ataatccatg gccagtttcc tttgcaagca cgaagtgaat ccttaaaagg aattggcttt
    13141 tgagtttctg gatgaaagtt gcgctctctt aaagttgtaa aagcgaagat caacattgcc
    13201 gctgcaagaa ttccaaatag ggcaatggta accatgaatc caactttgtc atttttctct
    13261 ccagttaatg cataaccaaa agcaccaacc agcggaatgc aaagtgagca agtaataaaa
    13321 ctccccaaac ttccaccagc catacgaaat gtatttgcgt taatgcgttc acggttactg
    13381 cttgtaaggt ttggcaaaat tgcagtaatt gcagtttgaa caccagtgta aacaatgcct
    13441 gcacttaaaa tataggtaat cattgcgtac caaaacttcg gagctccatc cggtaagtct
    13501 ggtgcaaaaa acgccaacca tacagttaca acaaatggtc ctgttagcca taagaatcat
    13561 ggtcgggatt gacctcattt tgtttttgtg tgatcaatga ttgaccctca cacaattgcg
    13621 cttaatgcat cagcaaaacg agcaacaagc aaaataatac ttgcgccaag cgctcctgat
    13681 gctggaattc caaatacatc agtatagaaa tacatgatgt aagttgaaat tgtaacgtaa
    13741 agcaagttgc ctgccatgtc tgtgcaagca taaccacact tttctttaag cgataacttt
    13801 acgtctcctg tgtctttcgt aacctctttt ggttttgcca tttttactcc tttattcgtt
    13861 taaatccatt tttagtcagc cttacctgac ttattgtttc gttttttttg caaacgaaat
    13921 gtcaatcttt gtctaagaaa tgaaattctt ttggaaatgg cgcatagctt ttaattatgt
    13981 agccatcttt tgtttgaact ggaactccat ttacaacata gataatacct tgcaattcat
    14041 aaacaaatgt ttttgtacca aagccatcaa ggtatgcaca catttcttcg cccatgtctc
    14101 aatccatggt gctatcgtca tttgcaaata gcttgctaaa tattcctcca agctctgcga
    14161 agacagattg tccaaaccta tcgcgcgaaa tttttgggaa gttaattagt ggtccagcaa
    14221 ctgaatatgt cgaagctgct tcgcctttgt tcaaccttaa gttgcatgca tttgcgtgag
    14281 tgtcataaca atataaaatt gcaactgttg cataatatgc ggcatcaagc atctttcaat
    14341 catttaatgc ttcataacac ttaagcatac ttgcagcaga aattcctcct cacaaagaat
    14401 gcgtcatata agaaagcgcc tctcaccatc tgtgagggtt ttgagcgcga aaatcatttg
    14461 aataaccaat gttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncttatgt
    14581 aaatctggat taatacgaag ttcaaaaacc tgtagcgctc aatccatata agttttagca
    14641 ttattcaatt ttaaatattt gttaggaatt tgcgaaagca agaagaaggt gtgagcgata
    14701 tattcaaaat ccataacacg ataaaaactt ccataaagtt ttacaggctt taaaaagtct
    14761 ccattttcaa atcacttttg tttaatgtaa taaacagcgc tttcttcaac ttttttaata
    14821 tgtgaagggt taaatttatt tgccaaaatg ctgtacttta aaacaaggtt taacttaccg
    14881 atgctctcgc cctggtttga aacagggtaa aagcagtagg ggactttatc atttttaccg
    14941 tgatacaaag tatcacaaat ataatcacaa cgttctttaa gaagttgcgg aattttatcc
    15001 atgacattta gaacaacgca atcactacag ccgtcatcca ttactacttc tattttatgc
    15061 tctccttgtt catgaacttg gaagtaaata aaacctccag taggctcata atacttatgc
    15121 ggcaattcat acttttgaag atagccattt ttattaaatc aaacaaaaat atttgtaatt
    15181 cggcgacgaa gtggtaaacg cacctcaagt gcaacataac cacctgcatg ggtataaggg
    15241 ttataaccta tgtatggcat tccaagattt ttgccctgag ttccaaaatc atttttatct
    15301 ttgaatgaac aaattgttca agtccattct tttgttgtat ttgcaggaag atcgataggg
    15361 ctatgatcat aaattcaatc gtgatgagga acgtcctttt caacataacc accggctaaa
    15421 actaattggt gaaacaaaat tccatcaagc gacggagaaa cattttcaaa aaataaatta
    15481 gaatattcgc aaaaggttcc aacagatttt gtttctcctc atgtttgata cattccaaga
    15541 tttggacctt tgttgttgtt acggaccatt gcaagacgag taaaatcttt agaaatagaa
    15601 ggcgttactg tgcaactttc attaatgttt tttaaaacat ttttatcacg atacattata
    15661 tgagcaaaag gagtccatac tctaaagtca ttaatttgaa aattttcatt ggttttatta
    15721 gtaagttgaa ctttaaaatc aagaccttcg tattcggcat cgcgtaagtt ataaataact
    15781 tccattttta cgcgcctatc atcataaatt accgaagtcc tgtaatcctc ggttacaact
    15841 tttcggtgct ttgcattaat agatcttcgc ttttcgccaa aagtaacaac ttcaaattcg
    15901 ccaaaaagtt tatcttcatc cttaaaacca acttttttta gataggcagg atcaacaact
    15961 cagttcatat tgtacttgtc gccattttga taaagtccaa ttaagttacc ctttttgttg
    16021 attttaatat taaatgcttt attttccatg ttaactcttt ttagcttaat aaatttagtt
    16081 gaatttttct gttaagtctt tttcagctgg aagacctttc ttgtaggctt ctataaattt
    16141 atcgtaacca gctacttcat cagcatttgc ttcaacacta atgacgccat aagattcaaa
    16201 aatcttattt ataaagtcag ataagctttc gttgccatct tttgcactga atgctgcaag
    16261 aagtgcgatg cctcatgcgc caccttccga agcagtactg aaagtttcaa tgtttgtgtt
    16321 aagtgcagct gccaagaaac gttgagccac gccttcggtt ttaaaaattc cgccatggca
    16381 aaccatcttg tcaatttttg caccttcgtc tttaataaga atatccatac caattttcat
    16441 tgcagcaaag caagaataaa tttgtgcgcg aatgaagttt gcaagattga aattcgcttt
    16501 ggttggatga atgaaacttg ggcatccttg ttcaagtcca acaatgtctt cgccagaatg
    16561 aaaacaatat ggcaaaagct ctccgcaatc tgcgtcgccc ttaagtgaat tttcaaatag
    16621 caaagtgtag agttcgttag tgtcaacatt tgaaccacca agttccaaaa cttctttgaa
    16681 taatgacacc caagcgttaa tttctgttgt gcaattgttc gcgtgtgaca tagctacaag
    16741 gtctccagct ggggtagtaa ccatatcaat tgttgtgtgg agctccttta gatttttctc
    16801 taaaacaagc attgcaaatg ccgaagttcc agcagaaata tttccagttc ttttagcaag
    16861 actgttagtt gcaaccattc cagttcctgc gtctccttct ggcggacaag caattgcgcc
    16921 accttgaagt tttcctgatt tatcaagaag ttttgcacct tcgtcagtaa gcgttccggc
    16981 attttcgcca gcaactaaaa cttttggcaa aatatctttt aaattccaat caaaattttt
    17041 gtctttaatc aaatcactaa atttgtttaa catctcttcg tcataattat gagtctcaag
    17101 attaataggg aacattcccg aagcttcgcc aactcctgca agtttttcgc cagttaattt
    17161 tcaatgaaca taacctgcaa gtgttgttag aaagtcgatg ctcccaacat ggtcttcgcc
    17221 atttagaatt gcttggtaga tgtgagcaat gcttcaacgt tgagggatgt tgtagttaaa
    17281 taattgtgtg agctcatcag cggctttgcc cgtgttattg tttcttcaag ttctaaattc
    17341 agcaagttga ttaccttgct tatcgaaggg aaggtaacca tgcatcattc cgctaattcc
    17401 aaatgcttta agctttgtaa tttcagcggc atatttctct ttgacgtctt tgcacaagtc
    17461 ttcataacaa cttgcaagcc ccgaccaaac ttcgtcaaga ggataagttc aaagattatt
    17521 ttcgaagtgg ttttcccaat tgtggccgcc tgttgcaaga acttcgccgg cttcatcaat
    17581 aagcacagcc ttgattcttg ttgagccaaa ctcaacacct aaaataccat cgccattttc
    17641 aattatggtt ttattctttt cgttatccat tttacttaaa actccatttc ttctcgattt
    17701 gttccaaaga ttcaccctga gtttcaggaa ctctgaattt tacaaataaa actgctaata
    17761 aacaaattat accaaagatt gcaaatacta tatcctggct catgcttgct gtcataattg
    17821 ggaaaaatag accaacagca aatgatccaa gtcaattcat tgacgaagca aggccgctag
    17881 ctcttcctct aattccaaga gggaatattt cgccaacaat aactcatgta agaggcgctc
    17941 atgtaaatga atagaacgct acatatatgc acaaaaatgc aataatcatt actttaggga
    18001 aggcagatcc taaaagagtt ccaagaattg caggcattaa aaacgatagc cccattataa
    18061 ttccgcccgt aataatcatt gaacgccttc taaatttatc agcgatagcc atataaaaga
    18121 tagcgccaga aactaatatt gcaccttgaa ttactggcca gagtagagca tttgaagccg
    18181 ccactcctgt tgcttcttcc acaatcaaag gaatgtaata aaagatagca ttggcgccca
    18241 ttatttgttg taagaatgct acactgacac ccgcaattac aaggtatctg tatctttttg
    18301 caaacaaata tctaaaagtt atcttctctc ttgcttcgct ttcttctttt gctgtcttaa
    18361 taatttcttt tagctccggc tcaatttcgt ccttttccct tattaaagtt aatacttctt
    18421 tagcttcttt ttcaaaacct tttttaacta aaaatcttgg cgattctgga agtctcaaaa
    18481 ctccaagcaa taatatgata gccggcactg ctgcaaggcc aagcatcaac cttcatgaaa
    18541 tattatttgg aagaccttgc aatgcgtaat cgacaatata tgatagaagc attcctgaaa
    18601 caatcattaa ttgatttaag ccagaaagca ttcctcgctt attcgccggt gccatttctg
    18661 acatatacga aggcaccaat gccgaagcag ctcccacgcc aagccctaat aaaatccttg
    18721 atgcaagaag ccattcaacc atttgatttg gtgagaaagc agccattaca gctcctattg
    18781 caaatacgaa tgatgaaaac aagatgactt ttctccgtcc gtatttatca gaaaagtggc
    18841 ccgctaaaat tccaccgaag attgccccca acattaagat tgaagtaatt caaccgatga
    18901 gtgccgggtc ttcgatgcct caatcaattt ttaaaaatgg tagtgcgcct gtcatgacgc
    18961 cgatatcgta tccaaataaa atgcccccaa atgcgccgaa aaagtagata aactttcgcg
    19021 aaatcgtcct tggcgtattt gacactttaa cctcctggtt taagaatgga ggcgacatcc
    19081 ggattcgaac cggagataaa ggctttgcag gcctctgcct taccacttgg ccatgtcgcc
    19141 ctataaaaaa aatggagcgg acgacggggt tcgaacccgc gaccccaacc ttggcaaggt
    19201 tgtgctctac ca
//
LOCUS       DBNCDC010000008        18857 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_792_length_18857_cov_5.613818, whole genome shotgun sequence.
ACCESSION   DBNCDC010000008 DBNCDC010000000
VERSION     DBNCDC010000008.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 18857)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18857)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18857
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_792_length_18857_cov_5.613818"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="AC04VQ_01310"
     CDS             <1..417
                     /locus_tag="AC04VQ_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013334.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HIO9552880.1"
                     /translation="IDPGLKISSRSQSSADRGQSKGEAVHEVGVISAILKKVEKVMDD
                     EDLSTCDTVVLEVGELSGVVPHFMEECWPAATYHTRFEETKLEMKVIKGQVRCDSCGE
                     EFNAYECDLKCPKCGEDRKHTPLTGRELVIKEIRGN"
     gene            419..1075
                     /gene="hypB"
                     /locus_tag="AC04VQ_01315"
     CDS             419..1075
                     /gene="hypB"
                     /locus_tag="AC04VQ_01315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HIO9552881.1"
                     /translation="MSVKIIEVKESIFKNNNERADILRKRLKDEKTFLLNLMSSPGSG
                     KTTTLLRTCEQLKDKFKIGIMEADIDGSVDAETMKAAGVKSIQIHTGGMCHLDADMTA
                     QGIDELGTSDVDLAVLENVGNLVCPAEFDTGAVKNACILSICEGHDKPLKYPLIFETC
                     DAMIINKMDVMPYFNFDMDQVKEYAYKRNPNIKIFPISAINGEGVDAWCDWLTQQVND
                     "
     gene            complement(1200..1583)
                     /locus_tag="AC04VQ_01320"
     CDS             complement(1200..1583)
                     /locus_tag="AC04VQ_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552882.1"
                     /translation="MFLPYLNLVTDYAKGFKYEKRFDTLTKKSPRDPEGKPIKYDESI
                     AALCFKRLSAVAQDDRKLYSHGIRKHWSKVESEQIANYPKVIDKTYKLYKKDKKFAIK
                     YINKYTIDMQSNVLAEAKSMFDELV"
     gene            complement(1611..1847)
                     /locus_tag="AC04VQ_01325"
                     /pseudo
     CDS             complement(1611..1847)
                     /locus_tag="AC04VQ_01325"
                     /inference="COORDINATES: protein motif:HMM:NF015536.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1863..2090)
                     /locus_tag="AC04VQ_01330"
     CDS             complement(1863..2090)
                     /locus_tag="AC04VQ_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552883.1"
                     /translation="MKMPEDKMSVFGNCFKIQTEYDGLSEDEFRHSKDLFTLPESKKL
                     GVYKNGKLSLAETYEGKGELSEYDTVREYFG"
     gene            complement(2727..4148)
                     /locus_tag="AC04VQ_01335"
     CDS             complement(2727..4148)
                     /locus_tag="AC04VQ_01335"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HIO9552884.1"
                     /translation="MCTQVYIGKDVSADGHAIYARSCDLSSNKNTITTPLCSPKVENK
                     PGRQVKSIESKIVYDYPDTTYKCLTTPIIPATDYGKLTSIGLNEHGFIVTGTITAYNC
                     PEIAKVDPFLDDGICEETLPDFIGKTCKTPKEAIDFIEKTMEEKGNYSANSLIVADQK
                     EGWLIELYSGHQWAAIKLPTDKISVYGNLFMIQTEYEMDNPETFRHSKDLFKMPTDAG
                     LAVMKDGKMSLCATYGGKDRLEDYSNLRTYMGHYYFAKSTAGEYHTKTRYPLFFDPDK
                     KVSLKDVFNIYRFRYEGTPYCPEETGCENIRQIATEATFNCHAIQVFEDEKPQLASVA
                     WLSIANPEHSTFLPYSNMITKLDPRLDKFDELTNKLNDNKFLKDIEDMPIMYSEDIAQ
                     VIFKRLCIISEHDRKLYGKGVQEYWGKKEDKLIKEFPKVLAKASKLAEKSLDEAIDYI
                     NDWTLNLQSETIDEAKTIFDQLI"
     gene            4192..5994
                     /gene="lepA"
                     /locus_tag="AC04VQ_01340"
                     /pseudo
     CDS             4192..5994
                     /gene="lepA"
                     /locus_tag="AC04VQ_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803165.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
     gene            6120..6290
                     /locus_tag="AC04VQ_01345"
     CDS             6120..6290
                     /locus_tag="AC04VQ_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552885.1"
                     /translation="MGILGEESKFAESIIRFIFKNEDPPGPGPVPPGPTPPDPPGPGP
                     SPTPGGGGGTEW"
     gene            6402..7973
                     /locus_tag="AC04VQ_01350"
     CDS             6402..7973
                     /locus_tag="AC04VQ_01350"
                     /inference="COORDINATES: protein motif:HMM:NF015347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HIO9552886.1"
                     /translation="MVRTAENPGIGSVVSTLISQNSMLKVFAIVISCLVAVFACTSVA
                     NAGIKAVADESTPLSALAQVQTAYPSDVLMQADTTSQLAYADEPEPQPEPKAGISLES
                     GPYIDITVYKDGTLGIPDVADKVKNIDTTNGYFYSLEVSSNKQEIKEAIWKFTSGNTV
                     LFNQIANKANETNFSLPIEKAQTINIALSISNLSKETALSLVAGAGDKPVEVAKVRYM
                     ADGNVMDAPSKDATHGVLEEAFNKDGHDPTFATSVEFVKGNAPAEGTYRSKADVSRAG
                     SGAITAYMVNNEDGGYRIVVNSDDDIICAPSTCSRLFQNFRNLNYVDFENLNTSTCTD
                     FSYMFENCGRDDGEASMLDFSKPFRHEGKFKTGENASIAAMFYGCDCSVDATNLDTSK
                     VVKMDSLFENYADNVVDAARPFISLKLADGENKFVIPKDCTVDYMFKGCNYDLTNGPA
                     GPGEDAHNLLEDLDTSNSEHFARMFAAYAYVPPTVEPEPTPGNEDEPTAILDVSPLNV
                     SHAKDMSQMFMFTYA"
     gene            8067..8378
                     /locus_tag="AC04VQ_01355"
     CDS             8067..8378
                     /locus_tag="AC04VQ_01355"
                     /inference="COORDINATES: protein motif:HMM:NF015347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HIO9552887.1"
                     /translation="MYGFFETTNIINLDLTPINFSKVTSMDSMFAHYGFLNINVDKYL
                     VYHPEMSVTWPQSTADNPYPAAGCCADYMYCATSIKPEVLAKWDGTKITSAKGMFLEY
                     G"
     gene            8475..8801
                     /locus_tag="AC04VQ_01360"
     CDS             8475..8801
                     /locus_tag="AC04VQ_01360"
                     /inference="COORDINATES: protein motif:HMM:NF015347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HIO9552888.1"
                     /translation="MFHQAKCAVDLTNWDATNISNFGHMFLSFGNLQREKDFSIKFGE
                     EGNLFSPKAGASFEGMFEMVQLSNVDLSYVDLDKVNDFSYMFEGFGQVVALSKEVTKP
                     FEGHIT"
     gene            8964..10877
                     /locus_tag="AC04VQ_01365"
     CDS             8964..10877
                     /locus_tag="AC04VQ_01365"
                     /inference="COORDINATES: protein motif:HMM:NF015347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HIO9552889.1"
                     /translation="MFKGFNGLSIFGAKYNGINLITPEEGLATGENANLSHMFEGSQV
                     YTLEPTNFDKAIDPEVDIQRNVYKSVGMALKDVIKTNNAVNMSYMFADTYSQSLSKLD
                     GEKEYTYRYSSIDVSGCDISKVTDMSYMFAGAFWKMPPYQDIVGNPTKMAGLLTLFDK
                     PTNDDVLYINASHMFDGCSAESIDIDNLPMNRFNDVSYLFAFYGSVNCYGIVDNLDKL
                     TFANNAKVAGLFFMSFIKSRDGSEISLDLKGIDVSEITDMSYMFFNFKPAKLDISTWN
                     PKACTNMEGMFMSFGKDFSTSSQSGSVDPTTFSPTVLELPKTLSKDTGWHTGLNCNMK
                     WMFALCSADVDLSGFDVSKVTDMTGMFMCYGSSVNDVAFVLKNIAANVLQYIKNLLSQ
                     IMKTPSMSLEGISEEDMSLAVLACLIDGQGSDLVDIASLPTLQEYRNKISDGTSAIDY
                     LSSDYASSVKIATEGITSVEINNEYIEDGADAVKFDKGSANEIYNDLYSNSAISSSYY
                     NSSLIGAYNSTSNSYTNDLKNAINDVLADSEFNEFFAFAGLSSVDVSNSSVPNMNLKP
                     FKITIPEDFCPASDEFLLNKRLCSMKGMFALCNLTNIQLEEGKTINDYMPDLTCLDTT
                     YVCDMSYMFTGYY"
     gene            10905..12185
                     /locus_tag="AC04VQ_01370"
     CDS             10905..12185
                     /locus_tag="AC04VQ_01370"
                     /inference="COORDINATES: protein motif:HMM:NF015347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HIO9552890.1"
                     /translation="MTKYDPHEVREIDVSSFNTANIGPYSPGENTADQKVGSMNMMFA
                     LTGSVVRDGKETDPSKSNHYRTVYKFGDDFVITSNCSTKFMFLYAKSDTLDLSNIDVS
                     EATDLSFTFALCNAPTLDLSAWGSADKLVSTQYMFALYGMIGVALEKQMTFTVKLGSS
                     DNKFKLYYQKPLSNEYTDTDTKGMFFVPGFTQLDLTNVDISKRKSLSRMFEFTILPVA
                     AAQSSSTLATNKALNISTWSTSNISDFSYMFAISFIPGITLPTAENFKTATSGAKLDY
                     MFMLSYTTQMTDQYPSGLDLSNFDVSNATSTKAMFYAYAFVIPMNFQANNIPVVKFGN
                     YNMTKLKDASWMFALSSFSVDISGLSASAVQNLEYMFAFTGLNPYERFLYLILQKRDN
                     TKDVNEEIVPLGDKIGAFYTEITLPPTTKEGKAS"
     gene            12231..13079
                     /locus_tag="AC04VQ_01375"
     CDS             12231..13079
                     /locus_tag="AC04VQ_01375"
                     /inference="COORDINATES: protein motif:HMM:NF015347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HIO9552891.1"
                     /translation="MSGQLDKKGITKVPYATYYPSELSLDFLDTSNVVNMESMFMLAQ
                     NSFKLEQLDTSKVTSTKKMFTAYGFISMLNYKSREKIDYAASLTFAKTNSKFKLNGAC
                     DTSYMFSLFLALPDIEMVDKKPVPTYFGLSGIDVSNLINFNSMFAFGLGVFNFDLSSW
                     DLSKAKNMDKMFLGYGMLARLSFDFDMIPFSFMGKQLKLPKKFPVVQTGEASCSAEWM
                     FGFACIVDCGSNDEDIYGTTLDLSAYDFSGIQDASHMFCGYNLNYNGLSEYGLDNLSL
                     LLQNIA"
     gene            13122..13544
                     /locus_tag="AC04VQ_01380"
     CDS             13122..13544
                     /locus_tag="AC04VQ_01380"
                     /inference="COORDINATES: protein motif:HMM:NF015347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HIO9552892.1"
                     /translation="MLAAASNADPKPIDIQKAGTTIKFSQDSTKPLKFANKADLSAMF
                     AFCDSNIEMQNVDVSNAASCNSMFALFGYSYDVKATTQKTCVLKIPNNLKFGTGCDVK
                     QMFMLYFPFTLDVSKFDVSAVKDMTRMFAFCRVQDLNI"
     gene            13647..15152
                     /locus_tag="AC04VQ_01385"
     CDS             13647..15152
                     /locus_tag="AC04VQ_01385"
                     /inference="COORDINATES: protein motif:HMM:NF015347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HIO9552893.1"
                     /translation="MKCMFTAYSNYKQTTAVYFPETDELVWPDGCDATAMFAACDARS
                     LMLHGLNVANFSSVDYMFAGCMNLERINVDFSTDWTQYGLAGSYMFYGCQKLTGQNDT
                     IYKQYHTNIEFARVDGLDNRPGYFTGIATLDEPEAKQVSAEIKSIFREKGRFTDSELS
                     SVTSVEFVKAKPSSTEKSISNIDGMITASISSGKMTIKCEGTVYARATSCIHLFQDFS
                     SLQSIDFNNMFDCVAAISTEGMFDGCTRLNNLDVSKLRIGNVENFSYMFENFGSGVTE
                     NAKVIVTFNTSDVDTFDTYSASNFSGMFKGAKIDSIDATKFSFMHATNLSSMFEGCLA
                     TEIDFPGRELDRYDTIKYEHADIKNGNLTNLFKNCKNVKSLIIGGFTPYSCDLSSAFC
                     GCESLKSIYIPVNHGDWSDNNAKTTKSPVFDNKCTNLTNDVGLNLSELNDYTVSACCP
                     CYYDNNKSRSGGIFSDYAVIGEPDNPSTTTVGIIQQRFGKTEIVKAKEFNI"
     gene            15258..15659
                     /locus_tag="AC04VQ_01390"
     CDS             15258..15659
                     /locus_tag="AC04VQ_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552894.1"
                     /translation="MSYVPKGLYVEGTSPYYLVIYSTYHIRSCTNSCSFMFANFKDLE
                     SAYLDTDSFAVLNKYKYQRIEDEEKTWDLYVGNPANLSYMFYNCISLKNFSFNKDPYV
                     YNNIMGYLGSTSHMFQNCTSLENINVEYFMP"
     gene            16431..17177
                     /locus_tag="AC04VQ_01395"
     CDS             16431..17177
                     /locus_tag="AC04VQ_01395"
                     /inference="COORDINATES: protein motif:HMM:NF015347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HIO9552895.1"
                     /translation="MIDTSAAVDCSFMFYRSGSNKVFYANDDINTTVDLTSFDTSNVK
                     SFKSMFSNSRFTNITMTTLSNEEKGLFENVASFLAGESNDNEEQSRFTLKSATNLNRM
                     FYGCGKTIMPNPFNVGTLSIYMPTEIKFPLNEQTVGVGCDVSEMFMLARICDLDISSL
                     NLNNAINFEKMFAFAGAINIDIKLPMKSKAEMIYNLALALDSSDEQSTINALQAFYDN
                     QVDINSLSENLGKPHKNFIGIEIYFNNKND"
     gene            17223..18305
                     /locus_tag="AC04VQ_01400"
     CDS             17223..18305
                     /locus_tag="AC04VQ_01400"
                     /inference="COORDINATES: protein motif:HMM:NF015347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HIO9552896.1"
                     /translation="MGCLTRNIDLTHFSTKNIENMSRMFMLTPLTGRYEVSSESFSTS
                     KVKSMWQMFAYAGRITGTSTFGYPIPGGADFPVFQFKQNCEVAQMFSNAYIPVDLSDC
                     KPAGETNGCGLNNIYDYSDMFYNFAINALPSVAIPSSEEEDPEPIGHPENLKIKLPSS
                     GWTPPAGSYMNEMFRGCKANVDLSGIESFENVVDMSRMFMSYGNGDVTWMGYEQVGIT
                     LPKTQGFKISTADNATMAEMFKGYKGLDVKEEGSEDTRASFQEIINCLDTSKVVDMSE
                     MFCGAKVENINLNTANFDTTNVRNMYYMFGSQLLRESYVKTITLGDKFKIDNVVDMRY
                     MFGSVGDYNMALRTIYYAGTNMKNAD"
     gene            18360..18803
                     /locus_tag="AC04VQ_01405"
     CDS             18360..18803
                     /locus_tag="AC04VQ_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552897.1"
                     /translation="MFYACLNLVGGRNTKAFTLYNVDKVDHSLARPAGIQGDTIVNGY
                     FTVADPEKAYPNKLEDDNNKTSLEDNNANNNTSTDSSEIIDSNEKIEDVALDEPVEEE
                     SIVELESPFDKLEVLLEDPSEVKVVALTFFQSIFNGLTKILLNIN"
BASE COUNT         5877 a         3169 c         3793 g         6018 t
ORIGIN      
        1 attgaccccg ggctaaaaat atcttcccgg agtcaatcga gtgctgaccg cgggcagagt
       61 aaaggagaag ccgtgcacga agttggcgtt atatctgcga ttttaaaaaa ggttgaaaag
      121 gtcatggatg acgaagacct ctcaacctgc gacactgttg ttttagaagt tggtgagctg
      181 tctggtgttg ttcctcattt tatggaagaa tgctggccag ccgccactta tcatactcga
      241 ttcgaagaaa ctaagctcga gatgaaagtt attaaagggc aagttcgctg cgacagctgc
      301 ggcgaagaat tcaatgccta tgaatgcgac ttgaagtgtc caaaatgtgg cgaagacaga
      361 aagcacactc cgctaactgg acgagaatta gtaataaaag agatccgggg gaattaatat
      421 gagcgtaaag attatagaag tcaaagaaag tatttttaaa aataacaatg agcgagctga
      481 tattcttcgt aagcgtttaa aggatgagaa aacttttctc ttaaatttga tgtcgtctcc
      541 aggtagtggc aaaactacga cacttcttcg gacttgtgag caactaaaag ataaatttaa
      601 aattggtatt atggaagccg atattgatgg aagcgttgat gctgaaacca tgaaggctgc
      661 cggagttaag tctatccaaa ttcacacagg aggtatgtgc cacttagatg cggatatgac
      721 tgctcaagga attgacgaac ttggaacaag tgatgtcgat ttggcggttc ttgaaaatgt
      781 tggcaatttg gtttgtccgg cagaatttga cacaggtgcg gttaaaaatg cttgcattct
      841 ttcaatttgc gaaggccatg acaagccgtt aaaataccct ctaatttttg aaacttgcga
      901 tgccatgatt atcaacaaaa tggatgttat gccatatttt aacttcgaca tggaccaggt
      961 aaaagaatat gcttataaga gaaatcctaa cattaaaatt tttccaataa gcgccataaa
     1021 tggcgaaggc gttgacgcct ggtgcgattg gttaacacag caagttaacg attgaattaa
     1081 ttaagatact tttctttata acacaaagta ggctgaaacg gttttggatc gtttcttcgt
     1141 agcttatgag tttttgaatc gcgtacatat ttaagcgtct caatatgtga agaaatatat
     1201 caaacgagtt cgtcaaacat tgattttgct tctgccagga cattgctttg catgtcgatt
     1261 gtatacttat taatatactt aattgcaaat tttttatctt ttttgtacag cttatacgtt
     1321 ttgtcgataa cttttggata attagcaatt tgttcgctct caacttttga ccaatgtttt
     1381 ctaatgccat ggctgtataa ttttcggtca tcttgcgcaa cagcagacag acgtttaaag
     1441 cataaagctg caatgctctc atcatatttg attggcttac cttcaggatc ccggggactt
     1501 tttttggtta gcgtgtcaaa acgtttttca tatttaaaac ccttagcata atcagttacc
     1561 aaatttaaat agggaagaaa caacgaatgt tctgcattac taacacaagc tcacataaca
     1621 attgccattt cagaaggcag attttcaatt atctctaaag cctgaataga gtaggtaact
     1681 tcggtgccaa tcagtcttgt tttttcaata ccctcatttt cttcgggaca aaatttgctt
     1741 ccttcgtaac gatgtctgta aagatcaaaa acatctttca atttaacttt tttttcgggc
     1801 ttaaaaaaca aatcatattt gcgtacttta tatttgtctt taatcgaagg gcaaaagaat
     1861 tttcatccga agtattctcg aacagtatca tattcgctaa gctcgccctt gccttcgtaa
     1921 gtttctgcaa gactcaactt gccattttta taaactccta atttttttga ctccggcaaa
     1981 gtaaacaaat ctttcgagtg gcgaaattca tcttcgctca gtccatcata ttcagtttga
     2041 attttaaagc aatttccgaa gacggacatc ttgtcttctg gcattttcat agctgctcat
     2101 tgatgtccgc tataaagctc aataattcaa gcttcctttt ggtctgccgc aagaataatt
     2161 tcagcataat tagagccaaa tttattcatg agtttttcaa ttaaatcaac acattctcgt
     2221 gctgttttac atgtaggacc caatatatta ttgataacag gctcgcaaat tccattttct
     2281 gttcaagggt caagtgctaa aattttgtcg cttgtagcac ctgtaattgt cgaggttacc
     2341 gcaagtccgt attcatttgc cgctgcatca ctcatgggtc cgcgtgccga aggaataaca
     2401 ggtagcgcat ttcatctgta tgtggttttc ggcaacttgt atttaaattt tgtttcggga
     2461 gttacaacat atttgttgtt gttctttttt cgaggatagc taaaaaatgc tgtcatgcta
     2521 tcgcgtcgct gtcaagtatc agcagatctt gcaattatag tgtgaccatt cgcactgact
     2581 tttttaccaa cataaacgcc tgtacacata atatatcctt atttaattac tggcttgaat
     2641 ggctcaagat gcttcttttc tttttcttga gcaaaatttg caaaactaaa catataacga
     2701 agagtctcaa tatgtccact gatgtatcaa atcaactggt cgaagattgt cttcgcttcg
     2761 tcgattgttt cactttgaag atttagcgtc caatcattaa tgtagtcaat tgcttcgtca
     2821 agagattttt cggcaagctt cgaagcctta gccaaaactt taggaaattc cttgattagc
     2881 ttatcttctt tcttgcccca atattcttga acacccttac catataattt acggtcatgt
     2941 tctgaaataa tgcaaagacg cttaaagata acttgagcga tgtcttcgga atacataatt
     3001 ggcatgtctt cgatatcttt aagaaattta ttatcgttta atttattggt tagttcatca
     3061 aacttatcaa gccttggatc tagctttgtt atcatgtttg aatatggcaa gaatgtcgaa
     3121 tgctccgggt ttgcaattga gagccaagca acagatgcaa gctgtggttt ttcatcttcg
     3181 aaaacttgaa ttgcatggca gttaaatgtc gcttccgtag ctatttgacg aatgttttca
     3241 cagcctgttt cttcagggca gtatggcgtt ccttcgtagc gaaaacgata aatattaaat
     3301 acatctttga ggctaacttt tttatcagga tcgaagaaaa ggggatatct tgtctttgta
     3361 tgatattcgc ctgcagttga tttagcgaag taataatgcc ccatgtaagt gcgaagattc
     3421 gaatagtctt cgagcctatc ttttccgcca taagtagcgc aaagggacat tttcccgtcc
     3481 ttcataacag caagaccagc atctgttggc attttaaata ggtctttcga atgcctgaaa
     3541 gtttccggat tgtccatttc atattcagtt tgaatcataa acaagtttcc ataaactgaa
     3601 atcttgtcag ttggaagctt tatggcagcc cattgatgac cagaataaag ttcaattaac
     3661 catccttctt tttggtcggc aacgataagt gaattagctg agtagttgcc tttttcttcc
     3721 atagtttttt ctatgaaatc tatggcttct tttggagttt tgcatgtttt tccaataaag
     3781 tcgggaagtg tttcttcgca aattccatca tctaaaaatg ggtcaacctt agcaatttca
     3841 ggacagttgt aagctgttat tgtccctgtc acaataaaac catgttcgtt taagcctatt
     3901 gaagttagtt taccgtaatc agttgctgga ataattggag tagttaagca tttgtaagtt
     3961 gtgtctgggt agtcataaac aattttgctt tcaattgact tgacttgtct accaggctta
     4021 ttttcaactt taggactaca aagtggagtt gtaattgtgt ttttatttga ggataagtcg
     4081 caagaacgcg cataaattgc atgtccatct gcgctaacat cttttcctat ataaacctga
     4141 gtacacataa caacgcgcat tataacactt tatgataaaa tagggcgact aatgagtgat
     4201 gatatgaaac atattagaaa tttttcgata attgcccaca tcgaccacgg taaatctaca
     4261 atatcagaca gaattttaga aatgactgga actgttgagg cgcgcgatat ggaaagtcag
     4321 cttcttgaca atatggatat agagcgtgag cgtggaatta caattaaaag tcaggcagtt
     4381 cgagtcaaat acaaggctga tgacggacaa gaatatcaat tcaacctcat tgatacacca
     4441 ggtcatgttg actttactta cgaagtttcg cgaagtcttg cagcttgtga tggtgctgtt
     4501 ttggttgtag atgcaactca aggtgtcgaa gctcaaactg tagcaaacgc tgctttggcc
     4561 attaattcaa accttgaaat tattccacta attaataaga ttgatcttcc gtctgctgag
     4621 cctgaaagag tcaaaaaaga aattgaagag agcatcgcta tcgaggcatc ggacgccgtt
     4681 ttggcatcgg ctaaaacggg cgaaggcgtc cacgacctgc ttgagtcaat tgtttataat
     4741 atcccagcac caaagggaga cccgtcttcg ccgctaaagg cattaatttt tgattcttat
     4801 tttgaccctt ataggggagt tgttgccttg attcgcgtca tggatggctg tattaaagac
     4861 aagcaatcca ttaaaatgct tgcaacaggc aacactgctc ttattgaaga atgtggtttt
     4921 aaaaatccga aggaaacacc ttgcggcagt ctcagtgcag gcgaggttgg atacattgtt
     4981 acaggactta aaaacgttag tgaaattaaa gttggggata caatcaccga agttgatgcc
     5041 aaggatgttt ctgcaattga gggttatcgt gaagttaagc caatggtttt tacaggaatc
     5101 tttccaatcg aaagtgacca gtatgagccc ctaaaagagg cacttgagaa gttgtcgctt
     5161 aatgatccgg cgcttgtttg ggaagctgag tcttcggtgg cgcttggctt cggatttcgt
     5221 gtaggctttt tgggactttt acacatggaa gttgtgaaag aaagacttga gcgagagttc
     5281 aatttggatt tgcttgcaac tgcaccaagt gttgaatata aggtatacac ctcaaaggaa
     5341 gttttaagcg ttaaatctcc tcaagacatg ccagaccctt ctgaaattca aaaaatcgaa
     5401 gagccatatt tgagcgcaag catattgatt cctccagatt atgttggggc agttatggac
     5461 ttgactgttt cgcgccgagg aatttttaaa acaatggatt atctttcacc aacaacagtt
     5521 gagatgaagt gagaaatgcc actgtcggag cttattttgg actattttga tcaacttaaa
     5581 agcagaacga agggcttcgc cagccttgac tatgaatttt taggttataa acaaagtgag
     5641 cttgtaaaac ttgatatttt gcttgcgggg gacattgtag atgcactttc gatgattgtt
     5701 cataaagata aagcatatga aactggacgt gttttgacct cgaaacttaa agaaattatt
     5761 cctcgtcagc ttttcgaagt tccaattcag gctgcaattg gaagcaggat tttgtctcgc
     5821 caaacagtaa aagccaagcg taaagacgtt ttggcgaagt gctatggcgg tgatgtttcg
     5881 cgtaaaagaa agcttttaga aaagcaaaaa gagggcaaaa agaagatgaa ggcaattgga
     5941 aatgtcgaag tccctcaaga agcttttatg gctattctaa aagttgacga ataacctgct
     6001 aaatctctta aaattcttgt ttaatttttt actttttcat atataatgag tgtttaattg
     6061 agggcctaaa ttttaggtcc ttgatatcta tgtcaaaaaa gtaaacgaaa gggaaaaaca
     6121 tgggtatttt aggagaagaa tctaaatttg cagaatctat tattaggttc attttcaaaa
     6181 acgaagatcc accgggaccg ggacctgttc ctccaggacc tacaccacca gatcctccag
     6241 gaccaggacc aagtcctact ccaggcggcg gtggcggaac agagtggtga aatgccgctc
     6301 ttacaggcga cactttaacg tggattttac ttgctattgt tgctgttgct tttattgctt
     6361 ttggtgttct tctttacaag aaatataagc gagatggagc aatggttaga acagctgaaa
     6421 atcctggtat aggttcagtt gtttcaactt tgatttcgca aaatagcatg ctcaaagttt
     6481 ttgctatagt tatatcgtgt cttgttgctg tatttgcatg cacaagtgta gctaatgcag
     6541 gtattaaggc agttgcggat gaaagcactc ctttaagcgc acttgcgcag gtccagacgg
     6601 cttatccatc tgatgttttg atgcaagcag acacaacttc gcaacttgct tatgcagatg
     6661 aaccagaacc tcagcccgag cccaaggctg gaatttccct tgaaagcggt ccatacattg
     6721 atattactgt ttataaagat ggcacacttg gtatccctga cgttgcagat aaagtaaaaa
     6781 atatcgacac cactaatggc tatttctaca gccttgaggt ttcgagcaat aaacaagaaa
     6841 taaaagaagc tatttggaaa tttacaagcg gtaacactgt tttgtttaac caaattgcta
     6901 acaaagctaa cgaaaccaat ttttctcttc caattgaaaa agcgcaaaca ataaatattg
     6961 ctctttcaat ttcaaatctt agcaaggaaa cagctttaag tttagtagct ggtgctggtg
     7021 ataaaccagt tgaagttgct aaagttcgtt atatggctga tggaaatgtt atggatgctc
     7081 cttctaagga cgcaactcat ggcgtattag aagaggcctt taacaaagac ggacatgatc
     7141 caacatttgc tacttcagta gagtttgtta aaggaaatgc tcctgcagaa ggcacatatc
     7201 gatcaaaagc tgatgtttca agagctggta gtggcgcaat tactgcatat atggtaaata
     7261 acgaagacgg cggatacaga attgttgtta attccgacga cgatattatt tgcgctccaa
     7321 gcacttgttc aaggctgttc caaaacttca gaaatctaaa ttatgtagat tttgaaaact
     7381 tgaatacttc aacttgtaca gattttagtt atatgtttga aaactgtgga cgtgacgatg
     7441 gcgaagcttc tatgcttgat tttagtaagc catttagaca cgaaggtaaa tttaaaactg
     7501 gtgaaaatgc aagcattgct gccatgtttt atggatgtga ctgtagtgtt gatgcaacaa
     7561 atcttgacac ttcaaaggtt gtcaaaatgg acagtctctt cgaaaattat gctgacaatg
     7621 ttgtagatgc tgcaagacct tttatttcac taaaacttgc tgatggcgaa aataaattcg
     7681 ttattccaaa agattgtact gtagattata tgtttaaagg atgcaattat gacctcacaa
     7741 atggtcctgc aggccctggt gaagacgctc ataacttact tgaagattta gacacaagca
     7801 attctgaaca cttcgcaagg atgtttgccg cttatgctta tgttcctcca actgttgagc
     7861 ccgagcctac tcctggcaac gaagacgagc caacagctat tttggatgtg tccccactta
     7921 atgtaagtca tgctaaagac atgagccaaa tgtttatgtt tacctatgct tgaaaaccaa
     7981 ttaacgacac ttccttaaat atcaagtttg gtgacgaagc aggcgacttc tatccgggca
     8041 gtgaaaacga aaaagcagaa gtaaacatgt atggtttctt tgagactact aatattatca
     8101 atcttgattt aacgccaatt aatttctcaa aggttacatc gatggattct atgtttgctc
     8161 actatggatt tttaaatatt aatgtggata aatatcttgt gtaccatcct gaaatgtctg
     8221 ttacttggcc tcagtctaca gccgacaatc cttatcctgc tgcaggttgc tgtgctgatt
     8281 acatgtattg tgcgacttct ataaagcctg aagtattagc caaatgggac ggaacaaaaa
     8341 ttactagtgc taaaggtatg tttttggaat atggctgaaa cgcaaattat ggattaatta
     8401 ctccaacacc agaaattcta aaagttgaat tcccaaccag ctacaagttt ggagatgact
     8461 gtgactgtag ttatatgttc catcaggcaa agtgcgccgt tgacttaacg aactgggacg
     8521 caactaacat ttctaatttt ggacatatgt tcctaagttt tggcaacctt cagagagaaa
     8581 aagactttag tattaagttt ggcgaagagg gcaatctatt ttctccaaaa gctggtgctt
     8641 cttttgaagg aatgtttgag atggttcaac ttagcaatgt tgacctatct tatgttgact
     8701 tagacaaagt aaacgacttc tcatatatgt ttgagggttt cggtcaggtt gttgcattaa
     8761 gcaaagaagt gacaaagcct tttgaaggac acattacttg aggcaatgaa gaaaggacaa
     8821 ttacacctgc acaaaagaca actgctgaag gtgaaaaggg cgctaactta agctatatgt
     8881 tcttaaatta taggttggct acatcgagca atgctgttga tctaagcaaa tgagacacca
     8941 agaacgttac aaacttcgaa ggtatgttta agggctttaa tggtttgtca atttttggcg
     9001 ctaagtataa tggcattaac ttaattacac cagaagaagg tttggcaacg ggtgaaaatg
     9061 ctaaccttag ccacatgttt gaaggttcgc aagtgtacac cttagaacca acaaactttg
     9121 ataaagcaat tgatcctgaa gttgatattc aacgtaatgt ctacaagagc gtaggcatgg
     9181 cgttaaagga cgttatcaag acgaacaatg ctgtgaacat gtcttatatg tttgcagaca
     9241 cttattcgca gagcctttca aaacttgatg gagagaagga atatacctat aggtattcaa
     9301 gtattgatgt aagcggatgc gacatttcaa aagttactga tatgtcttat atgtttgcag
     9361 gcgcattttg gaagatgcca ccttatcaag acatcgttgg taacccaact aaaatggcag
     9421 gtttgctaac attgtttgat aaacctacaa atgatgacgt tttgtatatt aacgcatcac
     9481 atatgtttga cggttgtagt gctgaatcta ttgatataga taatttgcca atgaaccgtt
     9541 ttaatgacgt aagctatttg tttgcttttt atggatcggt taattgttat ggtattgttg
     9601 ataatttaga taaattaact tttgctaaca atgctaaagt tgcgggatta ttcttcatgt
     9661 ctttcattaa gagcagggat ggttctgaaa tttctcttga cttaaagggt attgatgtaa
     9721 gtgaaataac ggacatgagc tatatgtttt tcaactttaa gcctgctaaa cttgatattt
     9781 caacctggaa tcctaaggca tgcacaaata tggaaggcat gtttatgtct ttcggaaaag
     9841 atttttcaac aagctcgcaa agcggcagtg ttgaccctac aaccttttcg ccaacagtgc
     9901 tagagcttcc taagactttg agcaaagata ccggatggca tactggcttg aactgcaata
     9961 tgaagtggat gtttgctttg tgcagtgctg atgttgactt aagtggcttc gacgtttcaa
    10021 aagtaacaga catgacagga atgtttatgt gctatggcag cagcgtgaat gacgttgctt
    10081 ttgttcttaa gaacatcgca gctaacgtat tgcaatatat caagaatttg ctttctcaaa
    10141 ttatgaaaac accatccatg agcttagaag gaataagcga agaggatatg tcacttgctg
    10201 ttttggcttg cttgatagat ggccagggca gcgatttagt tgacattgcg agcttaccta
    10261 cactacaaga ataccgaaac aaaattagtg atggaacaag tgcaatagat tacttgtcga
    10321 gcgattatgc aagttcggta aaaattgcta cggaaggtat tacttcggtc gaaatcaata
    10381 atgaatatat cgaagacggt gccgatgctg ttaaattcga taagggtagt gcaaatgaga
    10441 tttataatga tctttattca aattctgcta ttagctcaag ctattataat agctccttaa
    10501 taggcgctta caatagtact agcaattcat atacaaacga tttgaaaaat gctataaatg
    10561 atgttttggc agattctgaa tttaatgagt tttttgcatt tgctggatta agtagcgtag
    10621 atgtaagcaa ttcgtcagtt cctaatatga atttgaaacc ttttaaaatt acaattccgg
    10681 aagacttctg tccggcttcg gatgaatttt tgctcaataa gcgactatgt tctatgaagg
    10741 gcatgtttgc tctgtgtaat ttaactaata ttcaacttga agaaggaaaa acaatcaatg
    10801 actacatgcc tgatttaact tgtttagaca caacttatgt ttgcgatatg tcgtatatgt
    10861 ttactggata ctattgaaat ccttccttta acgtattgcc agatatgaca aagtacgatc
    10921 cacatgaggt tagagaaatt gatgtttcat cgtttaacac agctaatatt ggaccgtata
    10981 gtcctggaga aaacacggca gatcaaaaag ttggctctat gaatatgatg tttgctctta
    11041 ctggttctgt agtaagagac ggaaaggaaa cagacccaag taaaagtaat cattatagaa
    11101 cagtgtacaa gtttggagat gattttgtaa ttacttcaaa ctgtagtaca aaattcatgt
    11161 ttttgtatgc taagagcgat actttagatt tgtctaatat tgatgttagc gaagcaactg
    11221 acttatcatt tacgtttgct ttatgcaatg cgcctaccct tgatctttct gcttggggtt
    11281 ctgcagataa gcttgttagc acgcaataca tgtttgcatt atatggaatg attggggtag
    11341 ctcttgaaaa gcaaatgact ttcacggtta agcttggtag ttcagataat aaatttaaac
    11401 tctattatca aaaaccgctt tcaaatgagt ataccgatac tgatactaaa ggtatgttct
    11461 ttgtgcctgg ttttactcaa ttagatttga caaatgttga cataagcaaa agaaaatctt
    11521 tgtccaggat gtttgagttt acaatccttc ctgttgctgc agctcaatct tctagtactc
    11581 tagccaccaa caaggccctc aatatcagta cttggagcac gtcaaacatt agtgattttt
    11641 catatatgtt tgctattagt tttatacctg gtattacatt gcctactgca gaaaacttca
    11701 aaactgctac atctggagct aaacttgatt atatgtttat gcttagttac acaacacaaa
    11761 tgacagatca atatccttca ggattggatt tgtcgaattt tgatgtaagt aacgcaacaa
    11821 gcacaaaagc aatgttttat gcctatgcat ttgttatacc aatgaatttt caagctaata
    11881 atattcctgt agttaaattc ggcaattata atatgacgaa gcttaaggat gcaagttgga
    11941 tgtttgcatt aagttctttc agtgttgaca tttctggttt aagtgcttca gcagttcaaa
    12001 atcttgaata tatgtttgcg tttactggcc ttaatccata tgagagattt ttgtatctta
    12061 ttctccaaaa acgtgacaat actaaggatg taaatgaaga gattgttcca cttggagata
    12121 agattggtgc gttttacact gaaattactt tgccgcctac aaccaaagaa ggtaaggcaa
    12181 gttgaactac atctgaaaac gctagcacaa aaggaatgtt tttgtttacc atgtctggac
    12241 aattagacaa aaaaggtatt actaaggttc cttacgcaac gtattatcca tcagagctaa
    12301 gtttggactt cttagacacc tcaaatgttg ttaatatgga atcaatgttc atgcttgcgc
    12361 aaaatagctt caagctcgaa cagcttgata cttctaaggt aacaagcacc aaaaaaatgt
    12421 ttactgctta cggatttata agcatgttaa attataaatc tcgcgaaaag atagattatg
    12481 ccgcaagtct aacatttgca aaaactaata gtaaatttaa attaaatgga gcttgtgata
    12541 caagctatat gtttagtttg ttccttgcgc ttcctgatat agaaatggtt gataaaaaac
    12601 ctgttccaac ttattttggt ttgtcaggta ttgacgttag caacttaata aacttcaatt
    12661 ctatgtttgc tttcggttta ggtgtattta actttgattt gtcttcgtgg gatttgtcta
    12721 aggcaaaaaa tatggataag atgttcttgg gctatggaat gcttgcaaga ctaagctttg
    12781 attttgatat gattccgttc tcatttatgg gcaagcaact taagcttcca aagaaattcc
    12841 ctgttgttca aactggcgaa gcaagttgca gcgctgaatg gatgtttggt tttgcatgca
    12901 tagttgattg tggttctaat gatgaagaca tatatggaac aacgcttgac ttgtcggctt
    12961 atgatttctc aggaatccaa gatgcaagtc atatgttctg cggctacaac cttaactata
    13021 atggtttgag cgagtatgga cttgacaatc tatctcttct tcttcaaaat attgcttgac
    13081 agtatattga tgtttatcgg aagtatttta ataatcgaaa tttgttagcc gctgcctcaa
    13141 atgccgaccc aaaacctatt gatattcaaa aagcaggtac aacaatcaaa ttctctcaag
    13201 atagtacaaa gccgttaaag tttgcaaata aagctgattt atctgctatg tttgcttttt
    13261 gtgattcaaa tattgaaatg caaaatgttg atgtatcaaa tgcagcttcg tgtaattcta
    13321 tgtttgcttt gtttggttat tcttatgacg ttaaagcaac tacacagaaa acctgtgttt
    13381 taaaaattcc aaataactta aaatttggaa ctggttgtga tgtaaagcaa atgtttatgt
    13441 tatatttccc attcactctg gatgttagca agtttgacgt ttctgctgtt aaagacatga
    13501 caaggatgtt tgcattttgt agggttcaag atttaaatat ctgaaattga gatgtaagct
    13561 catgcgaatc tatggaagga atgtttgctg gtgcatgtgc taagaatatt gacatgacaa
    13621 agtgaaactt taagatggtt aaaaacatga agtgcatgtt taccgcttat agtaattata
    13681 aacaaacaac tgccgtttat ttcccagaaa cagatgaact tgtatggcct gatggctgcg
    13741 atgctactgc catgtttgct gcttgcgatg caagaagttt aatgttgcat ggtcttaacg
    13801 ttgcaaattt ctcttcagta gactatatgt ttgcaggatg catgaatctt gagagaatta
    13861 atgttgattt ttcaactgac tggacacaat atggtctagc gggttcttac atgttctatg
    13921 gttgtcaaaa attaactggt caaaatgata ctatctataa gcaatatcat acaaatattg
    13981 aatttgcgcg tgttgatggt cttgacaaca gacctggtta tttcactggt attgccacac
    14041 ttgatgagcc cgaagcaaag caagtaagtg cagaaattaa gtcaatattt agagaaaagg
    14101 gtcgctttac cgattccgaa ttaagcagtg ttacttctgt agaatttgtt aaagccaagc
    14161 caagcagtac agaaaaatca atttcaaaca ttgatggcat gattactgct tcaattagta
    14221 gcggcaaaat gactattaaa tgcgaaggta cggtttatgc aagagcaaca tcatgcattc
    14281 acttattcca ggactttagc tcacttcaaa gcatagattt caataatatg tttgactgtg
    14341 ttgctgcaat tagtaccgaa ggaatgtttg atggctgcac taggcttaac aacttagatg
    14401 tctctaagct tcgcattgga aatgttgaaa actttagtta tatgtttgag aattttggta
    14461 gtggtgttac agaaaatgcc aaagttattg ttacgtttaa cactagcgat gttgatacat
    14521 ttgatacata ttctgcttct aattttagtg gtatgtttaa aggtgcaaaa attgatagta
    14581 ttgatgcaac taagtttagt tttatgcacg caactaattt gtcttccatg tttgaaggat
    14641 gcctagctac agaaatcgat tttcctggtc gagagcttga taggtatgac acaattaaat
    14701 atgaacatgc tgatatcaaa aatggaaact taacaaactt atttaaaaat tgcaaaaacg
    14761 taaagtcttt gataattggt ggatttacac cttattcatg cgatcttagt tctgcatttt
    14821 gtggttgtga aagccttaaa tccatataca ttccagtaaa tcatggtgat tggagtgata
    14881 ataatgcaaa aacaacaaaa tcgcctgtat ttgataataa gtgcacaaat cttacaaacg
    14941 acgtcggttt aaatcttagc gaattaaatg attacactgt tagtgcatgt tgtccttgtt
    15001 attatgataa taacaagtca cgttcgggag gtatcttttc tgactatgca gttattggag
    15061 aacccgacaa cccatcaaca actactgttg gtataattca acaacgtttc ggaaaaacag
    15121 aaattgtaaa agcaaaagaa tttaatatat gagatgatgg tagcgattcg ggtaaatata
    15181 ccgaaccaga ttcaaattat gtcattgcta aagctgatat ttctaaaaaa gaggacaagt
    15241 ctgtttatgc gtataaaatg tcttatgttc ctaagggttt gtatgtggaa ggtacttcgc
    15301 cttattattt agttatttat tctacatatc atattcgttc gtgtacaaat tcttgctctt
    15361 ttatgtttgc aaactttaaa gatcttgaaa gtgcatattt agatactgat tctttcgcgg
    15421 tacttaataa gtacaaatat caaagaattg aagatgaaga aaagacctgg gatctgtatg
    15481 taggaaatcc tgctaatttg agttacatgt tctataattg tatatcttta aagaattttt
    15541 cattcaataa agatccttat gtatacaata atataatggg ttatcttggt tctacaagtc
    15601 atatgttcca aaactgtaca agtttagaaa acattaatgt tgaatatttt atgccgtgag
    15661 gaaaaagtaa agatccgagt attacacatg atgattatat taagagtttt acttgtgatt
    15721 ctatgtttga aggttgcagt aagcttaaaa atgtatttgc ctctaacgga atagggaaag
    15781 ggatttatga aacaaattga actgaatgta tgtctggtaa taatttgttt aaaggctgct
    15841 tggtactcca agcaaatggc ggaaaatgaa catacgctga gtatatgaaa aaacatccaa
    15901 ccactgagcc tggtaaggat actgctctta tcaataactc tcaaaatgag ggattcttca
    15961 cttatggtgc tcttatggag gagcaagatc caactttatg aaaagaagga agtgggaata
    16021 gaagctcatt tgtagcatat cttaaagata atccaacagt acatgtatta gaaaatcaaa
    16081 ttcttagcgt tgagttttac tacgattggc ttgagtcgaa tacatataaa aatttaagag
    16141 atactgagcc tcaaaaatta ttagagccaa tcagaattaa ttcaatatca aatagcccaa
    16201 tctatgctta tgtttattat gatgaatatg ttgaatctga gaagtcagaa tatgcaagat
    16261 atcacgtagt aatatctacc cctcatgata atcataacaa aatattcgct ggtcaatttt
    16321 catttacagg actattcgct catttaacca acttggagta tttctctatg gagccaggtg
    16381 agctacgtga tggaacatga gttgagcaga ctccaaaaac agttgatgat atgatagata
    16441 catctgctgc ggttgattgt tcttttatgt tctacaggtc aggtagcaat aaggtgttct
    16501 atgcaaatga tgatattaat acaactgtgg acctcacctc atttgacact tcaaatgtaa
    16561 agagctttaa gagcatgttt agtaattcta ggtttacaaa cattacaatg accacattaa
    16621 gcaatgaaga aaagggtctt tttgaaaatg tggcatcttt ccttgcgggc gaaagtaatg
    16681 ataacgagga acagagcagg tttactctta agagcgctac taatctaaac agaatgtttt
    16741 atggatgcgg aaagacaata atgcctaatc catttaacgt tgggactctt tcaatttata
    16801 tgcctaccga aattaaattc ccactaaatg aacaaactgt aggtgtgggg tgtgatgtta
    16861 gtgagatgtt tatgcttgct cgaatttgcg atttagatat atcttctctt aatttaaata
    16921 atgcaattaa cttcgaaaag atgtttgctt ttgcaggagc tataaatata gatattaaac
    16981 tacctatgaa gagcaaggca gaaatgattt ataatttggc acttgctctt gattcctcgg
    17041 acgagcaatc tacaattaac gctttacagg cattttacga caaccaggtt gatattaata
    17101 gcttaagcga gaatcttgga aagccacata aaaatttcat aggtattgaa atttacttta
    17161 acaacaaaaa tgattgagga ccagaattag aaggctataa tatttctgcc gattcgatgt
    17221 ttatgggttg tctaacacga aatattgatt taactcattt cagtactaaa aatatagaaa
    17281 acatgagcag aatgtttatg ttaacgccgt tgactggacg atatgaagta tcttctgaga
    17341 gctttagcac ttcaaaggta aaatcaatgt ggcagatgtt tgcttatgca ggcaggataa
    17401 caggaacttc aacttttggt tatcctattc ctggtggtgc agatttccct gtgttccagt
    17461 ttaagcaaaa ttgtgaagtt gcgcagatgt tctctaacgc ctacattcct gttgacttgt
    17521 cagattgcaa acctgctggg gaaacgaacg gttgtggcct aaataatatt tatgattatt
    17581 ctgatatgtt ctacaacttt gcaattaacg ctttgccttc agttgctatt ccatcaagtg
    17641 aagaagaaga ccctgaacct attggtcatc ctgagaactt aaagattaaa cttccaagca
    17701 gtggctggac tccacctgct ggttcataca tgaatgaaat gtttagagga tgtaaagcca
    17761 atgttgattt gtctggtatt gaatcgtttg aaaatgttgt tgatatgtca agaatgttta
    17821 tgagttatgg taatggcgat gtaacctgga tgggatatga gcaagttgga attaccttgc
    17881 caaagaccca aggatttaag atttcaacag ccgataatgc aacgatggct gaaatgttta
    17941 aaggatataa aggtcttgat gttaaagaag aaggctcaga agatacaaga gcttcgttcc
    18001 aagagatcat aaattgctta gacacaagca aagtagttga catgagcgag atgttctgtg
    18061 gggctaaggt tgaaaacatt aacttaaata cagccaattt cgacacaact aatgttagga
    18121 atatgtacta tatgtttggt tctcaacttc ttcgtgaatc ttatgtaaag actattacct
    18181 tgggagacaa atttaagatt gataatgttg ttgatatgag atacatgttt ggttctgttg
    18241 gcgattataa tatggcgctt cgcactattt attatgctgg cacaaacatg aaaaatgctg
    18301 actgagcaag cattattaat gagaagatta aaaataaagg tagtgtattt actgatcgta
    18361 tgttctatgc ttgcttaaat cttgttggag gcagaaatac taaggcattt actctttaca
    18421 atgttgataa agtagaccac agcttagcaa gaccagctgg catacaggga gatacaattg
    18481 tcaatggtta cttcaccgtt gcagatcctg agaaggccta tccgaataag ttggaagacg
    18541 acaataacaa aacttcgtta gaagataata acgcgaacaa taacacgtcg acagattcat
    18601 ctgaaattat tgatagcaat gaaaagattg aggatgttgc tctggatgaa ccggttgagg
    18661 aagaatctat tgtagaactt gagagtccat ttgataagct tgaagttttg ttagaggatc
    18721 cttcggaggt taaggttgta gcgttgacat tcttccagag catatttaat ggcttgacta
    18781 agattttgtt aaacattaat taacggatcg aagtaaaaca tttaaagcag gcaataatta
    18841 gttttgttgg ctgcttt
//
LOCUS       DBNCDC010000009        18767 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_797_length_18767_cov_7.978035, whole genome shotgun sequence.
ACCESSION   DBNCDC010000009 DBNCDC010000000
VERSION     DBNCDC010000009.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 18767)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18767)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18767
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_797_length_18767_cov_7.978035"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="AC04VQ_01410"
     CDS             <1..393
                     /locus_tag="AC04VQ_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylate symporter family
                     transporter"
                     /protein_id="HIO9552898.1"
                     /translation="TINMEGASITIITLTLACCHTLGINMDFGTALLLSLIAAISAVG
                     TSGVSGGSILLVPMACSLFGIGGDIAAGVVAVGFIIGVIQDSVETALNSSTDGLFTAA
                     VQLREQITSGKPYHKIVGGTLDDKSKTK"
     gene            complement(386..1204)
                     /locus_tag="AC04VQ_01415"
     CDS             complement(386..1204)
                     /locus_tag="AC04VQ_01415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIO9552899.1"
                     /translation="MTSAVAAEFNIPLVITCHGTDIQGYVATDRFHDYCNRAARAAGS
                     IICISDKNAAEVRENFPDDAEKAITMPNGYDSHVFYPEENNRAEVLAEFGITKEYKHL
                     VSFAGKFAHFKGIDILLRAAKIYENEDTATVLAGDGQLFDEMTALREELGLKNVYFIH
                     NQPHEKLRKLYNVAEVSLAPSRNEPFGLVVIEANACGTPVIGTNDGGIAGILTDKTGI
                     LIDPEDPDSLAREVKAVLNGEKVFDRNTCAEYTKQTFSQDSLILKTIDLYNSLI"
     gene            complement(1205..1585)
                     /locus_tag="AC04VQ_01420"
     CDS             complement(1205..1585)
                     /locus_tag="AC04VQ_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIO9552900.1"
                     /translation="MKILMINLMPLTGSGSGVYVENMSRALVRQGHEVCVIFIDNLPF
                     EDKGYPFKCHPIYFTADDGTKPDVQDEVLPFNFPCLTTHPRSVFNFRDLSEEQEAQYC
                     DAFRRAIAQEVEDFQPDLIHSGHI"
     assembly_gap    1630..1729
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1730..2778)
                     /locus_tag="AC04VQ_01425"
     CDS             complement(<1730..2778)
                     /locus_tag="AC04VQ_01425"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF010697.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIO9552901.1"
                     /translation="MIKLNTKNSGFGETELNSVKDQTLAAYKTLMERSGKGSEMLGWL
                     NLANEYDKDEFARIKTAAKKIQDSSEVLVCIGIGGSYLGARAGIEFSFGQFANQLAKK
                     RGAVEVYFAGSNISSTYVQNIFDIIGDRDFSVNVISKSGTTTEPGVAFRLFREKLEEK
                     YGKDGAKDRIYCTTDGEKGAMHDMAIAEGFERFVVPNATGGRFSCLTAVGLLPMAASG
                     MDIDKVMQGLADATKKYDNDDFATNDVMQYAALRYLMYKNGKAIEILANYEPSLMMFG
                     EWFKQLMAESEGKEHKGLFPTAANFSTDLHSIGQFIQDGTRNLFETVIWVDQPQADLT
                     IPNDENNVDSLNFTSG"
     gene            2872..3420
                     /locus_tag="AC04VQ_01430"
     CDS             2872..3420
                     /locus_tag="AC04VQ_01430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014601.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIO9552902.1"
                     /translation="MRAAIYGAGSLGTVLGAYITEAGGQIDLINRNKKHVEALRKNGA
                     KIIGTVQKNVKVKVLFPDEMEGKYDVIFLMTKQLFNEEVVTMLKKFLAKDGIICTLQN
                     GIPEPLIASIVGQEHTMGCTVEWGATLLEPGVVELTSDTDSLSFHMGKMEGVSDEQFK
                     MVKDILELMCPVEFEENLIGAR"
     gene            3433..3876
                     /locus_tag="AC04VQ_01435"
     CDS             3433..3876
                     /locus_tag="AC04VQ_01435"
                     /inference="COORDINATES: protein motif:HMM:NF020135.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HIO9552903.1"
                     /translation="MNATFSGLGTVVGGTFGSISESKAAKPIAVATMKECIDVGHAAG
                     VTFAPVQGKDITKLFYYKGGLKKAFAKLLLPIAMKKHRDIVPSMHQDICNGKKCEIEA
                     INGVVCEYGKKYKVPTPYNDKIVEIVKQINEGKLEAQMSNLELFD"
     gene            4112..4357
                     /locus_tag="AC04VQ_01440"
     CDS             4112..4357
                     /locus_tag="AC04VQ_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552904.1"
                     /translation="MLPILIAGQGGKMVGGTRGRVMGSIAILGCIAADYVQYQAALNE
                     VAVQTAMVEAGMLSEVTATASTTPMLMAAMIMGPFAG"
     gene            4394..4726
                     /locus_tag="AC04VQ_01445"
     CDS             4394..4726
                     /locus_tag="AC04VQ_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552905.1"
                     /translation="MPAGFEMLINNFSVGIFGMILAIIGYFAIGPFMSFILTILAGGV
                     DILVNAKLLPAIAVFLEPAKVLFLNNAINHGVFTPLGAQEVQDAGQSIMYMLETNPGP
                     GLGVLIAY"
     gene            complement(4807..5034)
                     /locus_tag="AC04VQ_01450"
     CDS             complement(4807..5034)
                     /locus_tag="AC04VQ_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552906.1"
                     /translation="MIGDEIKKEIPAAITTPTIEIQIPPFGLIIKNETIDPGLAGPIN
                     PAPIIMNQKIAQKLPTIGPIITSGFAKTYGK"
     gene            5015..5899
                     /locus_tag="AC04VQ_01455"
     CDS             5015..5899
                     /locus_tag="AC04VQ_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIO9552907.1"
                     /translation="MISSPIIKMSKGKSLEEATAQKDAMKAESKGAVASSVKKNSGDV
                     SKIIFACDAGMGSSAMGATKFAKRLKNDRPDITVKNSAIDSIPQDADIVVCQSTLADR
                     ASKKTPAGAQLVQLENFLGDPALDDLYNQLTSEKTNKEVKKMDNSKEKTGKNTRTKLL
                     KEGIKLGQKAEGKDEAIQASGELLKELGCVDDQYIAAMHDREKEVSTYLGMGIAIPHG
                     TSEAKKHVKNSGIVFMQYPEGVSFGDEKAYLIFGIAGVGDDHLNLLAKISERLEDEEL
                     VEKLKNTKDVDFVLKTFN"
     gene            5915..6439
                     /locus_tag="AC04VQ_01460"
     CDS             5915..6439
                     /locus_tag="AC04VQ_01460"
                     /inference="COORDINATES: protein motif:HMM:NF025384.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HIO9552908.1"
                     /translation="MDIEERKVFDPYISVEYRNLKSTKKSKKEHKLRRVYKRNVALSI
                     IYTIITFGIYGIFWQFEIAKESNALSKNDEGFCPALVVFFTIITCGIYGVYWGYKVGK
                     KHNQFYEAHGEQDKDYNVIYLLLLLFNYLLPILSLICYAIMQAKINTMLHIEEEQNPQ
                     GIYVRDSSIFNRPL"
     gene            6452..6667
                     /locus_tag="AC04VQ_01465"
     CDS             6452..6667
                     /locus_tag="AC04VQ_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552909.1"
                     /translation="MLLICQTIPDYFISFFEQMFIGAPGNPATPIVGDLMNGAIQNSV
                     DQIVYADSAFMIAHSILSILLIVFVLW"
     gene            6707..7000
                     /locus_tag="AC04VQ_01470"
     CDS             6707..7000
                     /locus_tag="AC04VQ_01470"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HIO9552910.1"
                     /translation="MILRNFGRACLFMLPGLLFIGINALGLNFDNFKIGIVLLGFVPG
                     FVEEITFRGMIIPNFMRIYNRSKGIWLSLFVSAGIFGLIHLANIFAGADFGTT"
     gene            7076..7270
                     /locus_tag="AC04VQ_01475"
     CDS             7076..7270
                     /locus_tag="AC04VQ_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552911.1"
                     /translation="MIICHGTVDALAMMADEALQQGAVQTQAFVFDLSIVPLLVVSIF
                     FIIYGIFICRPSKHEEICDL"
     gene            7294..7830
                     /locus_tag="AC04VQ_01480"
     CDS             7294..7830
                     /locus_tag="AC04VQ_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013403.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HIO9552912.1"
                     /translation="MLAVQFGAGNIGRGFVGALLRQSNYEVIFADVVDDIVNAINNQK
                     QFTVHITDVESEDILIDNVSAVDSKNDISNVLKDADLITTAVGVNILPKIAPAIAKGI
                     SAKHLDGIEKPLNIIACENAVLATDKLKNAVYDLLSDDDKKFADKHVGFANCSVDRIV
                     PPVCDAQGLNVCVERYFE"
     gene            7876..8439
                     /locus_tag="AC04VQ_01485"
     CDS             7876..8439
                     /locus_tag="AC04VQ_01485"
                     /inference="COORDINATES: protein motif:HMM:NF019736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HIO9552913.1"
                     /translation="MNFVDNLEAYVERKLFTLNTGHAICAYLGFINDKATIEEAINDD
                     NIYKIVKSAMIESGSGLCKKHGFDEDAHKEYIDKIIKRFKNPHLKDDVTRVGREPLRK
                     LSIEDRLVKPMLTAYECELPIDNLLIGIAAALHFNNPEDAESVLMQEKIAKFGLRDAV
                     ADITSIADKELLDRIEENYYKLIENNG"
     gene            8432..11329
                     /gene="uvrA"
                     /locus_tag="AC04VQ_01490"
                     /pseudo
     CDS             8432..11329
                     /gene="uvrA"
                     /locus_tag="AC04VQ_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
     gene            11329..12150
                     /locus_tag="AC04VQ_01495"
     CDS             11329..12150
                     /locus_tag="AC04VQ_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552914.1"
                     /translation="MGENVDIKQQRHRERIGLIVFIVAILGIFAAVFGYIMASHSLDL
                     AASGIDENVGQLDGYLTIIFEGDNTPRVEKTSTTEKILKQATEFFIGDDSKTTTSEPQ
                     TDKDIKTEKATTENVRDFYKDKDSTVCKIKCSDYAAYTSGEVFERGYWKIGVLSVTPE
                     ILSTQTVSTIPTQAFKAIQERIYKLRYEDEVDLVVVLSPDQKITKYIDKVDCAICKTV
                     TDDIPDEGKTVDNIFVMRVPNVGIIGTVLSSPAKMLQGHVYSKVEIKKDEEESQP"
     gene            complement(12201..12770)
                     /locus_tag="AC04VQ_01500"
                     /pseudo
     CDS             complement(12201..12770)
                     /locus_tag="AC04VQ_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013703.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
     gene            complement(12819..13037)
                     /locus_tag="AC04VQ_01505"
     CDS             complement(12819..13037)
                     /locus_tag="AC04VQ_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552915.1"
                     /translation="MGVGGSGLATILGQGAGAILVFCYFIFSKKSAFRLKISGLVPDA
                     KLMGRILLMGLASFAMNLASTIICIVFN"
     gene            complement(13215..13628)
                     /locus_tag="AC04VQ_01510"
     CDS             complement(13215..13628)
                     /locus_tag="AC04VQ_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIO9552916.1"
                     /translation="MDKKQQKNSKNNKLGTAKIGKLLVEFSVPAVAGMVLTSFYNLID
                     TAFLGQAFPDGSGVAVTTLALPIMLLLIGFTMLPGQGGNALAAILLGEGDNKRVEQCL
                     GNSFILLIIFSGIIALIGYFGIDFILLIIGTREEL"
     gene            complement(13628..14308)
                     /locus_tag="AC04VQ_01515"
                     /pseudo
     CDS             complement(13628..14308)
                     /locus_tag="AC04VQ_01515"
                     /inference="COORDINATES: protein motif:HMM:NF037962.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
     gene            complement(14321..14551)
                     /locus_tag="AC04VQ_01520"
     CDS             complement(14321..14551)
                     /locus_tag="AC04VQ_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552917.1"
                     /translation="MGSDFSFLNNILDIGREFIGDALIDTLEVIPFLFITYLILEFME
                     NRMSSKALEKFEKAGPLGPIIGSALGALPQCG"
     gene            14626..14719
                     /gene="ffs"
                     /locus_tag="AC04VQ_01525"
     ncRNA           14626..14719
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="AC04VQ_01525"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            14856..14984
                     /locus_tag="AC04VQ_01530"
     CDS             14856..14984
                     /locus_tag="AC04VQ_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552918.1"
                     /translation="MVVFIETGIVFFPFLPGDSLIFAAGVFSAEGGIPDGGQNLNI"
     gene            15042..15245
                     /locus_tag="AC04VQ_01535"
     CDS             15042..15245
                     /locus_tag="AC04VQ_01535"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIO9552919.1"
                     /translation="MARFFGERIIKSGKVKGLTKERMDKLNSFFDRYGGLTVVITRFM
                     PFFRTFAPFACGLGRMKFGVFTI"
     gene            15428..16918
                     /gene="dnaX"
                     /locus_tag="AC04VQ_01540"
     CDS             15428..16918
                     /gene="dnaX"
                     /locus_tag="AC04VQ_01540"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIO9552920.1"
                     /translation="MAEALYSKYRPKKFEDVVGQKHILQTLKNAITANKITHAYMFCG
                     PRGTGKTTMARLLAKACMCKNAGGTSPSADFCGKCEDCNDIASGIHPDVYELDAASRT
                     GVENVREEIISRVGFAATRGDKKIYIIDEVHMLSTAAFNALLKTLEEPPEHVIFILCT
                     TDPQKVPETILSRCQRFDFAAISATDIFERLKYVCNQESVTAEDEALRQIAQNAGGAM
                     RNALTSLEQAIAFTNGNITIANIKDNYASDADADFDTLIEAIGMRNLPGIFEWCENEA
                     KNGADFSKICYSLTEIIRNMYVKCAGGNLHAELPDYIDAHLGLFNTDRLHRIMLVLSD
                     LSGELKTSTNVRLSFEVAMAKISNPKSDLSLEALAERISNLEASKPSQVSEVPAAPAQ
                     TIEEEPEELQPEVTEEEVPQPMDETPVFETEEKTEERVIPKKVVNVVEDKKPEPEPEP
                     EIKMPARPEAVDFSQARQNLKEEANVVSPADAESKNLQSNTELQKL"
     gene            17048..>17238
                     /locus_tag="AC04VQ_01545"
     CDS             17048..>17238
                     /locus_tag="AC04VQ_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552921.1"
                     /translation="MGVLKKPDSMEKIEVEIKAVMGEDVGFNIEIDENSNEVFEPSFE
                     NNFRGKKQDADEDVFPEEET"
     assembly_gap    17239..17338
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17339..17464
                     /locus_tag="AC04VQ_01550"
     CDS             <17339..17464
                     /locus_tag="AC04VQ_01550"
                     /inference="COORDINATES: protein motif:HMM:NF014616.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIO9552922.1"
                     /translation="EDVEMLQDLVVAAVNEALRGMHAISEEKMSAVTGGMNIPGL"
     gene            17464..17562
                     /locus_tag="AC04VQ_01555"
     CDS             17464..17562
                     /locus_tag="AC04VQ_01555"
                     /inference="COORDINATES: protein motif:HMM:NF045310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552923.1"
                     /translation="MAKDDTHTIEKLLAELERMPGVGPKSAQRIAY"
     gene            17563..18066
                     /gene="recR"
                     /locus_tag="AC04VQ_01560"
     CDS             17563..18066
                     /gene="recR"
                     /locus_tag="AC04VQ_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIO9552924.1"
                     /translation="MLNTDEETAHNLANTISEVKDRVHFCKTCFNYSTGDECEICADA
                     SRDHSQICVVCEPRNIPPIERSLAFKGVYHVLGGEISPIDGIGPEDLNIQPLINRLSN
                     SEVSEVIIATNPNIEGETTASYLARQLKPLGVKLTRPASGIPVGGDLEFADEVTLGRA
                     FEDRREL"
     gene            18130..>18767
                     /locus_tag="AC04VQ_01565"
     CDS             18130..>18767
                     /locus_tag="AC04VQ_01565"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HIO9552925.1"
                     /translation="MEGIVVFKIVEKESLNPVVERITIEAPYVAAKCEPGQFVILRVD
                     EDGERIPLTVSDWDREKGYVSVCFQPVGATTEALNHKKKGESIADFVGPLGKETETDN
                     LKKVCVIGGGVGSAIALPICKKLHAKGVDVTAIVGFRSKDIVFYEDEFEANSKELIRT
                     SDDGTWGEKGVVTAPLERMLEAGEKFDQVIAIGPLIMMKFVCLTTKKYDQPT"
BASE COUNT         5713 a         3085 c         3896 g         5873 t
ORIGIN      
        1 actattaata tggaaggtgc gtcaatcaca attattactt tgacgcttgc ttgctgccat
       61 actcttggaa ttaatatgga ttttggaact gctcttttgc tttcactgat tgctgcaatt
      121 agtgcggttg gaacttcagg agtatctggt ggatcaattc ttcttgttcc gatggcgtgc
      181 tcgttgtttg gaattggtgg agatattgct gccggagttg ttgcggtagg ttttatcatc
      241 ggtgtaattc aagattcagt tgagactgcg cttaactcat ctactgatgg attatttaca
      301 gctgcggttc agcttcgtga gcaaattact tcaggcaagc cttatcataa aattgtaggc
      361 ggcaccctcg acgacaagtc taaaactaaa taagactgtt atataaatct atagttttta
      421 ggattaggct gtcttggctg aaagtttgct ttgtgtattc tgcgcaagta tttctatcga
      481 agactttttc gccatttaag actgctttga cttcgcgtgc aagtgagtct gggtcttcgg
      541 gatcaatcaa gattcctgtt ttatctgtaa gaattcctgc gattcctcca tcatttgttc
      601 caataactgg agtgccgcat gcgtttgctt cgataactac taatccgaaa ggttcgttac
      661 gtgaaggtgc gagagatacc tctgcaacgt tataaagttt acgaagtttt tcgtgaggtt
      721 ggttgtgaat gaagtaaacg ttctttaaac caagttcttc gcgaagggcg gtcatttcat
      781 cgaagagttg tccgtcaccc gcaagcacag ttgctgtgtc ttcgttttcg taaatcttcg
      841 ctgcgcgaag caagatatca attcccttaa aatgtgcaaa ttttcctgcg aagctaacta
      901 ggtgcttgta ttcttttgta attccaaact ctgctaagac ttctgcacgg ttattttctt
      961 ctggatagaa gacgtgagaa tcatatccgt ttggcatagt gatagctttt tctgcatcat
     1021 cagggaagtt ctcgcgaact tctgcagcat ttttatcgct aatgcaaatt atagaaccag
     1081 cggcgcgggc agcgcgatta caataatcat ggaatcgatc agttgctaca tagccctgta
     1141 tatctgttcc atggcaagtt ataactaatg ggatgttgaa ttcagcagct accgccgagg
     1201 tcaatcagat atggccgctg tgaattagat ccggttggaa atcttcaact tcttgtgcaa
     1261 ttgcgcgtcg gaaagcgtcg caatattgcg cttcttgttc ttcgctcaga tcgcggaagt
     1321 tgaaaacact tcttggatgt gttgtaagac aaggaaagtt aaatggtaag acttcatctt
     1381 ggacatctgg ctttgtgcca tcgtctgctg tgaaataaat tggatggcat ttaaatggat
     1441 agcctttgtc ctcaaaaggt aagttatcaa taaagataac gcaaacttcg tgaccttggc
     1501 gcacaagtgc gcgggacata ttttctacat aaacgccact tccagagcct gttagtggca
     1561 tgaggttgat cattaaaatt ttcattattc aacttccttt agtctatttt ctaaatcatc
     1621 tttcatatcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cgcttgtaaa
     1741 attcagactg tcgacattat tttcatcatt tggaattgtt aagtcagcct gcggctgatc
     1801 aacccaaatg actgtttcaa ataaattgcg agtaccatct tgaataaatt gaccgatgct
     1861 gtgaaggtca gttgaaaaat ttgccgcggt tgggaacaaa cccttgtgtt ctttgccttc
     1921 gctttcggcc ataagttgtt taaaccattc accgaacatc atcaaacttg gttcatagtt
     1981 tgccaaaatt tcgatagctt taccattttt gtacattaaa taacgaagtg cagcgtattg
     2041 cataacatcg tttgtagcaa aatcatcatt gtcgtatttt ttggttgcat ctgcaagtcc
     2101 ttgcataact ttatcgatgt ccatccctga agctgccatt ggtaaaagtc caactgctgt
     2161 taaacaacta aaacgaccac ctgtcgcgtt tggaacaacg aagcgctcga agccttctgc
     2221 gattgccata tcatgcattg cacctttttc accatcggtt gtgcaataaa ttctatcttt
     2281 ggctccatct ttgccatatt tctcttcgag cttttcacgg aaaagcctaa aagcaacacc
     2341 aggttcagtt gtggtgccgc ttttactaat tacatttact gaaaaatcac ggtcgccaat
     2401 aatgtcgaag atattttgaa cataagtact tgaaatatta cttcctgcaa aataaacttc
     2461 aacagctcct cgcttttttg caagctgatt tgcaaattga ccaaagctaa attcaatacc
     2521 ggcacgtgca ccaaggtaac ttccgccaat tccgatgcaa accaatactt cgcttgaatc
     2581 ttgaatcttt tttgcagcag ttttaattcg cgcaaactca tctttatcat attcatttgc
     2641 caaatttagc catccaagca tttcggatcc tttgccactt ctttccatta aggtcttata
     2701 agcagcaagt gtttgatctt taacagagtt aagctcggtt tcgccgaaac cagaattttt
     2761 cgtgtttagt ttaatcatgt tttctccttt aacgatgata ttttaacatt ttgattattt
     2821 aatttgtaaa taacatataa taaacaggtc aagtgattca aaggagaaat tatgcgtgct
     2881 gcaatttatg gagccggcag tttgggcact gttttgggtg cttatataac agaagctggc
     2941 gggcaaattg atttaattaa tcgcaacaaa aagcatgttg aagcacttcg taaaaatggt
     3001 gccaaaatta ttggaactgt tcaaaagaac gtcaaggtaa aagttttatt tccagacgaa
     3061 atggaaggca aatatgacgt tatcttttta atgacaaagc aactttttaa cgaagaagtt
     3121 gtaaccatgc ttaaaaaatt ccttgcgaag gatggaatta tttgtacttt gcaaaatgga
     3181 attcctgaac ctttaattgc aagtattgta ggtcaggaac acactatggg atgcactgtt
     3241 gaatggggcg ctacgctgct tgaacctggt gttgttgagt tgacttctga cacagattcg
     3301 ctgtcatttc acatgggcaa gatggaaggt gttagtgatg agcaattcaa aatggttaag
     3361 gatattttag aattaatgtg tcctgttgaa ttcgaggaga atttgattgg tgcgcgttga
     3421 agtaaacttc taattaatgc aacatttagc ggattgggta cagttgttgg cggtaccttc
     3481 ggttctatct ccgaaagcaa ggcagcaaaa ccaattgcag ttgcaactat gaaagaatgt
     3541 atagatgttg gacacgcagc aggagttact tttgctcctg tacagggaaa agatattaca
     3601 aaattgtttt actacaaagg cggtttgaag aaggcattcg caaaattatt acttccaatt
     3661 gctatgaaaa agcatcgcga tattgttcct tctatgcatc aagatatttg taacggtaaa
     3721 aaatgtgaaa ttgaagcaat taatggcgtt gtttgtgaat atggtaaaaa gtacaaagtt
     3781 ccaactcctt acaacgacaa aattgttgag attgttaaac aaattaacga aggaaagctt
     3841 gaagctcaaa tgagcaactt agaacttttt gactaggttt tatatgataa aataataggt
     3901 tggcaatttt attgattgct tgtatatgtg tgtagtggag gaataaatga aacaaaagat
     3961 tcagagtatg ggtcgagccc tgagcggaat ggttatgcca aatattggcg cctttattgc
     4021 gtgaggttta ttgaccgcta tatttttata tccaaatgga tgatttccaa atgaagcaat
     4081 ttctaaaatc cagccctata ttcttaattt cttgcttcct atattaattg caggtcaagg
     4141 tggcaagatg gtcggcggaa cccgaggtag agttatgggt tcgattgcaa ttttagggtg
     4201 catcgctgct gattatgttc aataccaagc agcgcttaat gaagttgctg ttcaaactgc
     4261 aatggttgaa gccggaatgc tttccgaagt aacagctact gccagcacaa caccaatgct
     4321 tatggcggct atgataatgg gtccttttgc tggttgagtt attaagaaat tcgatcaact
     4381 tatggaagga cacatgcctg ccggttttga aatgttgatt aataatttta gcgttggtat
     4441 ttttggaatg attttggcaa ttattggtta ctttgcaatt ggtccattta tgtcattcat
     4501 cctaacaatt cttgcagggg gagttgatat attagttaac gcgaagctac ttcctgcgat
     4561 tgctgttttc ttagaacctg caaaagtttt gttcctaaac aacgcaatta atcatggagt
     4621 ctttactcct ttgggagctc aagaagttca agatgcggga caaagtatca tgtatatgtt
     4681 agaaacaaat cctggccctg gcttgggtgt tttgattgct tattgagtat ttagtcaaga
     4741 taaggcagcg aaggattccg cgccgggtgc aattatcatt catttctttg gtggtatcca
     4801 tgagatttat ttcccatatg ttttggcaaa tccgcttgta attattggtc caattgttgg
     4861 taatttctgt gcaattttct ggttcatgat tatgggtgcg ggattgattg gccctgcaag
     4921 tcctggatca atcgtttcgt ttttaattat gagtccaaaa ggcggaattt gaatttctat
     4981 agttggcgtt gtaattgctg caggaatttc ctttttgatt tcatctccaa tcatcaaaat
     5041 gtcaaagggg aagagtttag aagaagcaac agctcaaaaa gatgcgatga aagctgaaag
     5101 caagggtgct gtggcttcgt cagttaaaaa gaattctggc gatgtttcaa aaatcatttt
     5161 tgcatgcgat gctggaatgg gttcttcggc gatgggagca acaaaatttg cgaagcgctt
     5221 aaagaatgac cgacctgaca tcacggttaa aaattccgcg attgatagca ttccgcaaga
     5281 tgcagatatt gttgtttgtc aaagtacttt agctgacagg gcttcgaaga aaacaccagc
     5341 tggtgcccaa ttagttcaac ttgagaactt tcttggtgac ccagcacttg acgatttgta
     5401 caatcaacta acttctgaaa aaactaataa ggaggttaag aaaatggata attcaaagga
     5461 aaagaccggg aaaaacacaa gaacaaaatt gttaaaagaa ggaattaagc ttggacaaaa
     5521 agcagaaggt aaggatgagg ctatccaagc ttcgggtgaa ttattaaaag agcttggatg
     5581 tgttgatgat caatatattg ctgcaatgca tgatcgtgaa aaagaagttt caacttatct
     5641 tggaatgggt attgcaattc ctcatggaac cagcgaagca aagaaacacg ttaagaacag
     5701 tggaattgta tttatgcaat atcctgaagg tgttagtttt ggtgatgaaa aggcatatct
     5761 aatttttggc attgcaggag ttggagacga ccatcttaat ttgcttgcta aaatttctga
     5821 gcgtttagaa gatgaagaat tggtcgaaaa actcaaaaac accaaagatg ttgattttgt
     5881 tcttaagaca tttaattaat agttcggagt aattatggat attgaagagc gtaaagtatt
     5941 tgatccttat attagtgttg aatatagaaa tttaaaatca acaaaaaaaa gcaagaagga
     6001 acataagctt cgtcgagttt ataaacgcaa cgttgcactg tctattattt atacaattat
     6061 tacatttggt atttatggta ttttttggca gtttgaaatt gctaaggaaa gtaatgcctt
     6121 aagtaaaaat gacgaaggtt tttgtccggc gcttgttgtg ttttttacaa ttattacttg
     6181 cggaatttat ggagtttact ggggatacaa agttggtaag aagcacaatc aattttatga
     6241 agcacatggt gaacaagaca aagattacaa tgtaatatat ttattacttc tcttatttaa
     6301 ttatttgttg ccaatactta gcttaatttg ctatgcaatt atgcaagcta agattaatac
     6361 aatgcttcat attgaagagg agcaaaaccc tcaaggtata tatgttcgcg atagtagcat
     6421 ttttaataga cctctatgaa gtacacttct tttgctttta atttgtcaaa ctattcctga
     6481 ttatttcatt tcgttttttg agcagatgtt tattggtgct ccaggcaacc cagctactcc
     6541 tattgttggg gatttgatga atggagcaat tcaaaattct gttgatcaaa tagtttatgc
     6601 tgacagtgca tttatgattg ctcatagtat attaagcatt ttattaattg tttttgtttt
     6661 gtggtgattt aagcttaggt ttaagcattc aaattatgaa ggcgtaatga ttttgcgaaa
     6721 ttttggtcgt gcttgcttgt ttatgttgcc aggcctttta tttataggaa ttaatgctct
     6781 tggattaaat tttgataact ttaagatcgg tattgtctta cttggttttg ttcccggatt
     6841 tgttgaggaa attaccttta gaggcatgat aattccaaac tttatgagaa tctataatag
     6901 gtctaaagga atttggttgt ctttatttgt ttcagctgga atcttcggtc taattcatct
     6961 tgcgaatatt tttgctggag cagattttgg aacaacttga ttccaggttt tctatgcttt
     7021 tgcaatggga attttatttg cgggtgtatt tatccgaacc ggtaatattt gacctttgat
     7081 tatttgtcat ggtactgttg acgctttggc aatgatggct gacgaagccc tacaacaggg
     7141 tgcagttcag actcaagcat ttgtgtttga tctttctatt gttccacttt tggttgtttc
     7201 tattttcttc atcatttatg gaatttttat ttgtcgtcca tcaaaacatg aagaaatttg
     7261 tgatttgtga agcgagaaat gaggcgacgc tatatgctag cagtccagtt tggtgctgga
     7321 aatataggtc gaggttttgt tggcgctttg cttcgtcaat ctaattacga agtgattttt
     7381 gctgatgtag ttgatgatat tgttaatgca ataaataatc aaaagcaatt cactgttcat
     7441 attaccgatg ttgaatctga agatatttta attgataacg ttagcgctgt tgattcaaaa
     7501 aatgacattt caaatgtttt aaaagatgca gatttaatta ccacagctgt tggcgtaaat
     7561 attttgccga agattgctcc tgcaattgca aagggcattt ccgccaaaca tcttgatgga
     7621 attgaaaaac ccctaaacat aattgcttgc gaaaatgctg ttcttgcaac tgataaatta
     7681 aagaatgcag tttacgattt gctaagtgat gatgataaaa agtttgcaga caaacatgtg
     7741 ggttttgcaa attgttctgt cgataggatt gtacctccag tttgcgatgc acaaggttta
     7801 aatgtttgtg ttgagcgtta ttttgagtga aatgtttgca aaaatgaact tgttggagat
     7861 gttgaaattg aaggcgtgaa cttcgttgat aatttagaag cttatgttga gaggaaattg
     7921 tttacgctaa atactggcca tgcaatttgt gcctatcttg gatttataaa tgacaaagct
     7981 actattgaag aagcaatcaa cgatgacaac atttataaaa ttgttaagtc tgcaatgatc
     8041 gaaagtggct ctggtctttg caagaagcat ggttttgatg aagatgcaca caaagaatat
     8101 attgataaga tcattaagcg ttttaaaaat cctcatttaa aggatgatgt tactcgagtg
     8161 ggacgtgagc ctcttcgtaa gctttcgatt gaagatagat tagtaaagcc aatgctgacg
     8221 gcttacgaat gtgaattgcc gattgataat ttattgattg gtattgcggc tgcacttcat
     8281 tttaataatc ctgaagatgc tgagagtgtt ttgatgcaag aaaaaattgc gaagtttggg
     8341 cttcgtgatg cagttgctga tattacatca attgctgata aagaattgtt agacaggatt
     8401 gaagaaaact actataaatt aatcgaaaat aatggctaag aacatttgca taaagggtgc
     8461 tcgtgagcac aatttaaagg atgttaatat tgacatccct cgcgataagt ttgtagttat
     8521 tactggactt tctggtagtg gaaaaagtag tcttgctttt gacacaatgt atgccgaagg
     8581 tcagcgccgc tatgttgaga gcctttcaag ttatgctcgc atgtttttgg gtcagatgag
     8641 caagcccgat ttggatagca ttgatggttt aagtcctgct gtttcgattg atcaaaaaac
     8701 aacaagcaag aatccgcgtt cgactgttgg aacaaccaca gaaatttatg attatttgcg
     8761 tttgttattt gcgcgtgttg gaattcctca ttgtccggag tgcggacgtg tgattgaggc
     8821 gcaaactact gatcaagtta ccgatgaaat tttaaaactt ggaaacggtg atgacgataa
     8881 tccaactcga gcaattatat tgtctcctgt tgtggcaggc aaaaaaggcg aatacacaaa
     8941 tttctttgct gatttattaa agcaaggttt tagccgcgtt cgtatagatg gtgaaattaa
     9001 aaaacttgac ggaaaaccaa ttcgtttaaa taaacaagtt aaacatttta ttgatgttgt
     9061 aattgacagg gttcaattaa agagtgatgc aacaagtaga attgccgatg ctgttgagct
     9121 tgctacaaaa ctttctgaag gccgagtttt ggttcaagtt ttagatgaaa aggattcgca
     9181 agtaaaggct tcaaaagttt caagtggggc aaaaggcgga attgccgaag gtgagcacat
     9241 ttttagttta gcacttgcat gtcctgaaca cggtcacagt atggacgaat tgaagcctcg
     9301 tgatttttcg tttaatgcac cttatggttc gtgtccggat tgtttgggta ttggttataa
     9361 agacgaagtt gatccaagtt tgattgttcc agacaagtct ctttcaattg ccgaaggcgc
     9421 gatggttccc ttcgtaagtg gaaattatta tccgcaagtt ttggctgctg tttgtaaaca
     9481 tgttggtgtt agtgttgata ctccgtggca agatttgccg aagcgagcta aagatgcgtt
     9541 gatgtatgga cttggggatg aagaacttcg tgtcgattat ataacggtgg aaggaaggca
     9601 cactcattgg tacattgatt gagaaggtgc gagcggtgca attaagtatc gtcttaaaaa
     9661 agcaaaaagt gataggcaac gcgaaaaatt tgagaagtac ttcgcaactc gcaagtgtga
     9721 aacttgtaaa ggccagcgct tgaagcctga aattttggct gtgacagttg gtggaaaatc
     9781 aattcatgac gttacaacgc taagtgctgc agagtgttac aaattctttg aacatattaa
     9841 tgattatcaa aaatttagtg atgctcaaca tcaaattgcc gatccaatta ttaaagagat
     9901 taaagctcgt ttaaaatttt tagttgatgt tggtttggat tatttaactt tggagcgtgc
     9961 tacagcaact ttgtctggtg gcgaagctca gcgcattcga cttgcaactc aaattggagc
    10021 tggacttgtt ggtgtgcttt atattttgga tgagccaagc atcggacttc atcagcgaga
    10081 taatgcacgt ttaattgcaa ctcttgaaca tcttcgtgat ttaggtaaca ctgtagttgt
    10141 tgttgagcat gacgaagaaa caattcgcag tgcagattat gttgtggata tgggtccgct
    10201 tgctggcgag catggtggtt atgttgtggc tgcaggaacg cctaaaaaga ttgagcaagc
    10261 taagaaatct attactggac aatatttaag tggtgcaaaa aagattgaga cgccgaagga
    10321 acgtcgttcg aaaaataatg gaaagcttgt tttgactgga gcaaagcaac acaatttaca
    10381 aaatgttaaa ttagaagttc ctctttctac ttttacagtt gtcactggtg tttcgggttc
    10441 tggcaaaagt tctttaattg cagacacttt agctccagcg ctttcaaata tgttaaatag
    10501 atctcacaga agcactggtg aatataaaaa gattagtggc cttgaatatt tagacaaggt
    10561 aattacaatt gatcaaagtc caattggtag gactccacga agcaacgcag caacttacat
    10621 tggtctatga gatgacattc gtgcattata tgcaagcact cctgattcaa agattcgagg
    10681 atattctcaa ggtcgttttt catttaatgt tcctggaggt agatgcgaag catgccgagg
    10741 cgaaggtcaa atcaaaattg agatgcactt cttaccagat atttatgttc cttgcgaaga
    10801 gtgtcatggc acacgttata acagagaaac tttgttagta aagtatcgcg gaaaaaatat
    10861 atcggacatt ttaaatatga cagttgatga agccttgaag ttttttgaaa acattccaaa
    10921 tattaagcga aaacttcaaa cactttatga tgttggtctt ggatatattc atttagggca
    10981 gcctgcaaca actttgtcgg gtggagaagc tcaacgcgtt aaacttgcaa gtgaacttca
    11041 aagagttcaa acgggtaaaa cattttatat tttggatgag ccaacaacag ggcttcactt
    11101 cgaagacgtt agacagcttc ttaatgtgtt aaatagactt gttgatagtg gaaatacagt
    11161 tttagttatt gaacataact tagatgttat aaaatgtgca gaccacatag ttgatcttgg
    11221 tcctgaaggt ggagatggtg gcggaaaaat tatagctaca ggtacacctg aagaggttgc
    11281 aaaaattaaa ggttcttgga ctggggaatt tttaaagagg attttataat gggcgaaaat
    11341 gttgacataa aacaacaacg tcatcgtgag aggataggat taattgtatt tattgttgca
    11401 atccttggaa tttttgctgc agtttttgga tacattatgg ctagtcattc gcttgattta
    11461 gctgcttctg gaattgatga aaatgttgga cagttggatg gatacttaac aataattttt
    11521 gaaggagata atacgcctcg tgttgaaaag acaagcacaa ccgaaaaaat cttgaagcaa
    11581 gctactgaat tttttattgg cgatgatagt aaaacaacta ccagtgaacc acagaccgat
    11641 aaagatataa aaactgaaaa ggcaacaact gaaaatgttc gtgactttta taaagataag
    11701 gattctacag tttgcaaaat taagtgcagt gattatgctg cttatacatc aggcgaagtt
    11761 tttgaacgcg gttattggaa aattggagtc ttaagtgtaa cccctgaaat tttaagtact
    11821 caaactgtta gcacaatacc aacccaagca tttaaggcaa ttcaagagcg catttataaa
    11881 cttcgttacg aagacgaggt agatcttgtt gtagttttat cgccagacca aaagattaca
    11941 aaatacattg acaaagttga ttgtgcaatt tgcaaaacag ttactgacga tattccagat
    12001 gaaggtaaaa ctgtagataa tatttttgtg atgagagttc caaatgttgg aattattgga
    12061 acagttttat cttctccagc gaaaatgctt caaggacacg tttattctaa agttgaaatt
    12121 aaaaaagacg aagaagaaag tcaaccataa tctattaatt agtgcaatta ggattgcgat
    12181 ttaccaagat tagtttcaat tcaagtttct ttttgttctt taatttttgc tttgagtttt
    12241 cgtccttcta ttatgataaa gcagattgtt aatagacttg agaaaaagtc tgctgctggt
    12301 ggacataaga ttactgcaat aagcccatct tgaattacac ctaaagataa aagtaattct
    12361 gggacaatca aatacatcgg aattaaaaag ataacttgtc ttgttaattc aagaattgca
    12421 gatttaatcg gctgcccgct gctttgaaaa taagtgccac ccacagcgtg gaaacctacc
    12481 aatgggaaaa atgtaatata taccattaat gttgtggccg aaaattcttc gagatctcca
    12541 acaatgccga acaattgaac aatttgatga ggaaataaat aacaaacaat taaaaagaac
    12601 gtgcctatag ctacacctgt tgtaattgac aaacgaagcg ctcgaataac tcttgttcaa
    12661 ttttgagctc catagttgta tccaatgatt ggttgagcac ccatggcaac accaataaga
    12721 ggcatgaaag caaaggttgc aactttttga gatacgccaa tacttgcaag tgcaccttga
    12781 gctccaagtg ttgatagtgc tccgtaatat ccaacaactc agttaaacac aatacaaatt
    12841 attgttgatg ctaaattcat tgcaaacgaa gctaatccca taagaaggat tctgcccatt
    12901 aattttgcgt ctggcactaa accagaaatt tttaatctaa atgctgattt tttagagaat
    12961 ataaaatagc aaaatactaa aattgctccg gcgccttgcc caagaattgt tgccagacca
    13021 cttccgccaa ctcccatatt taactcaaga acaaataatc aattaagaat gatgcaaaca
    13081 ataacactaa caacatttgt taaaagtgca attcttggtg ctccggcagt tctaataaag
    13141 ttgttcattc ccattccaac tgcgagaagg aaaaagccgt aacattgaat ttgaacaaaa
    13201 attttagttc cttctcatag ttcttctctt gtaccaatta ttaacaagat gaaatcaatt
    13261 ccaaaatatc ctatcaaagc aataattccg gaaaatatta ttagtaagat aaacgaattg
    13321 ccgaggcact gttcaacacg cttgttgtcg ccttcgccaa gaagaatagc agcaagcgca
    13381 ttaccacctt gtcccggaag cattgtaaag cctattaata aaagcattat tggaagtgca
    13441 agagttgtaa cagcaacacc agaaccatca gggaatgctt ggcctaaaaa tgcagtgtca
    13501 attaaattat aaaacgaagt tagaaccatt cctgcaacag caggaaccga aaactcaacc
    13561 agaagcttgc ctatcttcgc agttcctaat ttattgtttt tagaattttt ttgttgtttt
    13621 ttatccatta tcctacaatc aaagtcacta acattcctat aacagcacta ataacaaaaa
    13681 ttgctatagt gataataata ttttgcttca tcggtctatt gtttctaaac aatacaatta
    13741 aagctactcc tgttgcatca agcaatccag caagaagtgc acccattcca agaactccat
    13801 caacatataa ttgtgctatt acaacacttg atgcgcaatt aggtattagg ccaattaacg
    13861 atgttccaat tacgctcata aatctatttt catcaattaa gcttgcaaga gtttcataac
    13921 cgccgacaag aataattacg atgtttagaa gtaatgtaat tatgaagata aaaattgtaa
    13981 cttgaaatgt gtgaattatt gctgggcgca aaactttgct cttcgatttt ccatcatgat
    14041 gattgcaact gcatctatca cgttcgcaga gttcatgaat ctcatatctt tctttgcgct
    14101 ctgttttatc aagtttaaaa attttgaagc acccatctat tgcaaatcca acaattactc
    14161 caatggcaaa tttaactcca acaattccaa atagtgtttc taatggtatt ccgcctgcaa
    14221 tgaatatcgg aatcatttca tctgatgttg aaaggtaaac agcaaacaat gttccaattg
    14281 taataacttt tccagaaaag aatgtcgaag ctgccgaaga tcatccacat tgaggaagtg
    14341 cgccaagtgc tgaaccgata attggtccaa gtggtcctgc tttttcaaat ttttctaaag
    14401 ctttagagct cattctgttt tccataaatt caaggattaa ataagttata aacaagaatg
    14461 gaattacttc taatgtatct atgagagcat cgccaataaa ttctcgtccg atatctaaga
    14521 tgttatttaa aaaagaaaaa tcgcttccca cagcatatcc tttcttatat tttgcaagcg
    14581 cattataaca taattgctca cacttcatct tttttggtat aatttagttg ggctaaggcg
    14641 gcaacacgcc ttgaacctgg tcaggccaga gatggagcag ccataagggg cctctgatga
    14701 gcgttttagc ccattttttt tgctcaaatg aggtataaat ggaagtaata agcaatattt
    14761 ttaatttttt tgttaccctt ttaaaagatc caagaagtgt aattttgggt tgaattgcag
    14821 ttttaggacc tgttggtgtt tttacaccat tatttttggt tgtatttatc gaaactggaa
    14881 ttgttttctt cccatttctt ccaggagatt cattaatttt tgcagctggt gtgttttctg
    14941 ccgaaggagg cataccggat ggcggtcaaa acttaaatat ttgaatgact ttatttgtat
    15001 tttatgctgc tgcaattttg ggcaacactt cgaattattg aattgcaaga tttttcggtg
    15061 aacgcattat taaatctgga aaagttaagg gtctgacaaa agaacgcatg gataaattaa
    15121 acagcttttt tgatagatat ggtggcttga ccgttgtcat cacacgtttt atgccatttt
    15181 tcaggacttt tgccccattt gcttgtggtc tgggaagaat gaaattcggc gtgtttacaa
    15241 tttgaaatac aatcggtgga attgcttgag taactacatt tgcattaatt ggttatttct
    15301 ttggaaatgt tccgtttgtt caagaacatt ttagtgtaat tgttattgga atagtgttag
    15361 tatcagttat gccagcagtg attggcgtta ttaaagcatt cattgattcg cgaaagaaga
    15421 aaaaataatg gcagaagcac tatatagtaa atatcgtcca aagaaatttg aagatgttgt
    15481 tggtcaaaaa cacattttgc aaacactgaa gaatgctatt actgcaaata aaataacgca
    15541 tgcatatatg ttttgtggtc cacgaggcac tggaaaaacc actatggcgc gattgctcgc
    15601 aaaggcttgc atgtgtaaaa acgctggtgg gaccagtcct tcggcagact tttgtggtaa
    15661 gtgtgaggat tgcaatgaca tagctagcgg aatacaccca gatgtttatg agcttgatgc
    15721 tgcaagtaga actggtgttg aaaatgttcg tgaagaaatt ataagccgtg taggttttgc
    15781 agcaactcga ggggacaaga aaatttatat aatcgacgaa gttcacatgc tctcgactgc
    15841 ggccttcaac gctttgttaa aaactttgga agaaccgcca gagcatgtaa tctttatttt
    15901 gtgcactact gatcctcaaa aagttcctga aacaatttta tccagatgcc agcgtttcga
    15961 cttcgctgca atttcagcaa cagatatttt tgagcgcttg aaatatgttt gcaatcaaga
    16021 aagtgttacg gctgaggatg aagcacttcg tcaaattgct caaaatgctg gtggagctat
    16081 gcgtaatgcg cttacaagtt tagagcaagc aatagctttt acaaacggca atatcacaat
    16141 tgcaaatatt aaagataact atgccagtga tgctgatgct gatttcgaca ctttgattga
    16201 ggcaattggc atgagaaact tgcctggaat ttttgagtgg tgtgaaaatg aagcaaagaa
    16261 tggtgctgat ttttcaaaga tttgttattc gttaactgaa attattagaa atatgtatgt
    16321 taaatgtgca ggtgggaatt tacatgctga actacctgac tatattgatg cgcatttagg
    16381 cttgtttaac actgataggc ttcatagaat catgctggtt ttaagtgatc tttcaggtga
    16441 gcttaagaca agtactaatg tgcgtctttc ttttgaagtt gctatggcta aaatttcaaa
    16501 tccaaaaagt gatttgagtt tggaggcttt ggctgagcga atttcgaatt tggaagcttc
    16561 taaaccatct caagtcagcg aagttcccgc agctccagct caaacaatag aagaggagcc
    16621 tgaagaactt cagccggaag ttactgaaga agaggtgcct caaccaatgg atgaaactcc
    16681 cgtttttgaa actgaagaga aaacagaaga gcgggtaatt cctaaaaagg ttgttaatgt
    16741 tgttgaagat aagaaacctg aaccagaacc agaacctgaa attaaaatgc cagcgcgtcc
    16801 cgaagctgtt gatttttctc aagctcgaca aaatttaaaa gaggaagcta atgttgtaag
    16861 tccagctgat gctgaaagta aaaatttgca aagtaacaca gagcttcaaa aactttgaca
    16921 tactgcattt tcaaacattc gaagaaaata tcctgctttt ggagcaacgc ttttaacggc
    16981 taacccaagt tataatgata ttagcaatca attcatttta acttttccaa aaaatgcaag
    17041 ttttagtgtg ggagttttga aaaaacctga cagtatggaa aagattgaag tcgaaattaa
    17101 agctgttatg ggcgaggatg ttggttttaa catcgagatt gacgaaaata gcaacgaggt
    17161 ttttgagcct tcgtttgaaa ataatttcag aggtaaaaaa caagatgctg atgaagatgt
    17221 ttttccagaa gaagaaacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga
    17341 agatgttgaa atgcttcaag acctggttgt tgctgcagtt aacgaagcgc ttcgtggaat
    17401 gcatgcaatc agcgaagaga aaatgtcagc cgttactggt ggaatgaata ttccaggact
    17461 ttaatggcta aagacgatac acatactata gaaaagttgc tggccgaact tgagcgtatg
    17521 cctggggttg gtccgaagtc ggcacagcgc atcgcttatt gaattttaaa tactgacgaa
    17581 gaaactgctc acaatcttgc aaacacgatc agtgaagtta aagatagagt tcatttttgt
    17641 aaaacttgtt ttaattattc aactggcgat gaatgtgaaa tttgtgcgga tgcttcgcgt
    17701 gatcattcgc aaatttgtgt tgtttgtgag cctcgaaata ttccgccaat tgagcgaagc
    17761 cttgcattta agggtgtata ccacgtgctt ggtggtgaga tttcgccgat tgatggaatt
    17821 ggtccagaag atttaaacat ccaacctctt ataaataggc tttcaaattc tgaagtctcc
    17881 gaagttatca ttgcaacaaa cccaaatatt gagggtgaaa caactgcgtc ttacttggca
    17941 aggcaactta aacccttagg tgttaaactt acacgtccgg caagtggaat tccagtagga
    18001 ggcgaccttg aatttgctga cgaagtaaca ctgggccgtg ccttcgaaga ccgccgcgaa
    18061 ctttaatctc taaatagaat actatctgat ttaagaaatg ttgtataata cgctggtcaa
    18121 gtaaattaaa tggaaggaat agttgtgttt aagatagtag aaaaagaatc actaaatcct
    18181 gttgtcgaga ggattactat cgaggctcct tacgttgctg cgaaatgcga acctggacag
    18241 tttgttatct tgcgcgtgga tgaagatggc gagcgaatcc ctctaacagt ttctgattgg
    18301 gacagagaaa aaggttatgt aagcgtttgc tttcaaccag ttggagcaac aactgaggct
    18361 ctaaatcata agaaaaaggg cgagagcatt gctgattttg ttggacctct tggaaaagaa
    18421 actgaaacag acaatttgaa aaaagtttgt gtaattggtg gaggtgttgg ttctgctatt
    18481 gctcttccaa tttgtaaaaa acttcatgca aaaggtgttg acgtaactgc aatcgttggt
    18541 tttagaagca aagatattgt attttacgaa gatgaatttg aagccaactc aaaagaattg
    18601 attagaactt ctgatgatgg tacatggggt gaaaaaggtg ttgtaactgc tccattagag
    18661 cgtatgttag aagctggcga gaagttcgat caagttattg caattggtcc attaatcatg
    18721 atgaagtttg tttgcctaac tactaagaaa tatgatcaac caaccat
//
LOCUS       DBNCDC010000010        17451 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_940_length_17451_cov_7.345654, whole genome shotgun sequence.
ACCESSION   DBNCDC010000010 DBNCDC010000000
VERSION     DBNCDC010000010.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 17451)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17451)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17451
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_940_length_17451_cov_7.345654"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            96..818
                     /locus_tag="AC04VQ_01570"
     CDS             96..818
                     /locus_tag="AC04VQ_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIO9552926.1"
                     /translation="MFDVGNTPLVEFEKIRENENLACHIFGKMEYKNPAGSTKDRIVK
                     QILDDAIADGRLRDGDEIVEATSGNTGIALAAYGGMLGHAVKIFMPENMSQQRIDLMR
                     HYGAEVVLTPASENVEGSIAAVHEYIDTHEHCFLADQFNNLSNTTAHFENTAREIWDQ
                     TNGEVDAFIAGIGTGGTLKGVGDFLKEKNPNIYLAAVLPAESHHVIQGIGDGFDPPFF
                     KNNENINEVIKITNAEAMQYFN"
     gene            complement(1010..>1722)
                     /locus_tag="AC04VQ_01575"
     CDS             complement(1010..>1722)
                     /locus_tag="AC04VQ_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009608658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIO9552927.1"
                     /translation="DFSDFDITGRPNVHYVCLTEYDEKFTLPIAFPKEIPSNVLADVL
                     ADEGLVQYHTAETEKYAHVTFFLNGGVEEPKKNEIRKLIDSPKVATYDLQPEMSAPQV
                     VSTLSDAIAKDTADVYIVNFANCDMVGHTGVFDAAVKAVEAVDAGVGEVVSAIEAKGG
                     AVLITADHGNADCMIQDNGEPMTAHTTAKVPFVLLDFTGKNRELTAEDGALCDIAPTL
                     LHLISINQPKEMTGTPLL"
     assembly_gap    1723..1822
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1823..2712)
                     /gene="gpmI"
                     /locus_tag="AC04VQ_01580"
     CDS             complement(<1823..2712)
                     /gene="gpmI"
                     /locus_tag="AC04VQ_01580"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIO9552928.1"
                     /translation="MDKPICLIIMDGFGLADAGPKNAIASAKTPNLDKIFSEHKVTKL
                     DASGEEVGLPAGQMGNSEVGHLNIGAGRVVYQELTRINKACQDGSILNNEVLITACKH
                     AKENNGALHLMGLISDGGVHSSLEHLFALFDLAIAQEVPHVFVHCFMDGRDVPPTSGV
                     EYIGQLEDKIAIKNAQCVADGKKTSIAIASIQGRYYVMDRDKRWDRVQKGYDAIVKAQ
                     PCEETLSAARVCEKSYSVDVTDEFIVPTALINRGMKDGDSVVFFNFRPDRARQITHAL
                     TDADFSDFDITGRPNVHYVC"
     gene            complement(2770..3537)
                     /gene="tpiA"
                     /locus_tag="AC04VQ_01585"
     CDS             complement(2770..3537)
                     /gene="tpiA"
                     /locus_tag="AC04VQ_01585"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013979699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIO9552929.1"
                     /translation="MRKLVMAGNWKMNKTIGEAVSLAQEISNEYENDWAEKVEVITCT
                     PFVDLKPVVEVFNFDKKNIGVGAQNCYFEESGAFTGEISVPMLKEIPCSHCIIGHSER
                     REIFGETNEMINKKAKALIAADITPIICVGESLNVRDEGTYDEFVRKQVQAAFAGIDA
                     TDAEDCIVAYEPIWAIGTGRTATPEQAEEVCAAVRDEIKNIYDERVAENIRVLYGGSM
                     KPENAEGLLANENIDGGLIGGAALKADSFIALCKACL"
     gene            complement(3741..4604)
                     /locus_tag="AC04VQ_01590"
     CDS             complement(3741..4604)
                     /locus_tag="AC04VQ_01590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015760208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIO9552930.1"
                     /translation="MSDTNEKIAVIIPCYNEAITIGKVVDDFKRELPNADIYVYDNNS
                     SDDTAKIAKEHGAIVRTETRQGKGRVVRQMTRDITADYYVMVDGDDTYPAEAVHDLLA
                     PLRENLADMTIGDRLSNGSYGDENDRAFHGLGNNLVRWLIKMIYKFDFNDVMTGYRAY
                     NTVFAKALPILSPGFQIETELSIHAVDKSWRIVEVPIDYRDRPEGSESKLSTFGDGMK
                     VLTMILSLFKEYRPLMLFGIIALICFALGLICGIPVIVDYVNTGLVERFPTAILALGF
                     MLFALLNLITG"
     gene            4872..5132
                     /locus_tag="AC04VQ_01595"
     CDS             4872..5132
                     /locus_tag="AC04VQ_01595"
                     /inference="COORDINATES: protein motif:HMM:NF016060.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIO9552931.1"
                     /translation="MVGGTLGFTISVIFNYLASMKYVFKHKEDMSRTREFIIFVVLSI
                     IGLGINNACLWFLIDFIQWPFELSKPVLELGAKIIATAVVMV"
     gene            5268..6302
                     /gene="gap"
                     /locus_tag="AC04VQ_01600"
     CDS             5268..6302
                     /gene="gap"
                     /locus_tag="AC04VQ_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009307178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIO9552932.1"
                     /translation="MTVKVGINGFGRIGRMVYRAMAGDKRFDVVAINDLGNVEVMAHL
                     LKYDSIHGVAFKKVEAKGDTIVADGHEIKVLSERDPKDLPWGKLGVDVVIESTGLFTD
                     GEKAAAHIKAGAKKVIISAPATNVDGTFVMGVNDDTYDKEKHHIVSNASCTTNCLAPF
                     TKVLLENFGIKRGMMNTIHSFTGDQVVLDGPHKDLRRARASALNIIPTSTGAARAIGL
                     VLPELQGKLDGFATRVPTPDGSLVDLTAELEVPVTKEQINEVMKKAANGPLKGILEYA
                     EDPLVSSDIIDNPHSSIFDSKLTMVMPGGVVSKVVAGEDAGTMVKCVAWYDNEWGYSN
                     RAADLAAIML"
     gene            complement(6352..7266)
                     /locus_tag="AC04VQ_01605"
     CDS             complement(6352..7266)
                     /locus_tag="AC04VQ_01605"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIO9552933.1"
                     /translation="MANNLGRALIIVNPVAQSGKGSKNGYKTFETLRAEYHQDVDLFY
                     TKHDGHAMKIAEDTAKYDSVIVVGGDGVVHETASGIMRIPKAKRPIMGVIPVGSGNDY
                     ARSLGMSFNVHHAVDQLMHAKVKQVDVGQCNDKYFVETLSFGLDAGIAIDTMKARQKS
                     KEKGFKLYFKCGVDRIMNHFEFFDFEAKLDRGRKINGHSIIFAIQIGKTYGGGFKITP
                     DAELDDGYFNMCYSVGNLTRKTTLPLFARAAKGKHVGSKHVAFDKSKNIKLKINQLVP
                     CQLDGEEYSNHNFDIKTHSKALRVYVAS"
     gene            complement(7275..7409)
                     /locus_tag="AC04VQ_01610"
     CDS             complement(7275..7409)
                     /locus_tag="AC04VQ_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552934.1"
                     /translation="MLELVRPTAPLIFADAGPECVRTKCIEGDMSCGKPYPKVDRSKL
                     "
     gene            complement(7434..7973)
                     /gene="thyX"
                     /locus_tag="AC04VQ_01615"
     CDS             complement(7434..7973)
                     /gene="thyX"
                     /locus_tag="AC04VQ_01615"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HIO9552935.1"
                     /translation="METKINVKLLYHTKDPERAVATAARLCYSAVGAADLIEKMTDEQ
                     VAKILKIILAGGHSSTLEHASYTFAIEGVSRALTHQLVRHRMASYNQQSQRYVAVKDG
                     LSFVKPQTVKDNAEANQIFDEAIKSCEETYEKLVAAGVPKEDARFILPNAAETKIVVT
                     MNIRELLHFFSVRCCNRAQ"
     gene            8076..8486
                     /locus_tag="AC04VQ_01620"
     CDS             8076..8486
                     /locus_tag="AC04VQ_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552936.1"
                     /translation="MREFFDRLNIRIAEFMEGRYGLDNLNKFLLIFGFVLVFFGAIVS
                     PVDLLGWGFAIIALFRALSRNFDRRQIENEWYLKVTRAPKRFFKKISTRWKNRKTTVY
                     FKCSNCKQPLSVPKGKGKIRVICPKCGTEIYKTT"
     gene            8488..9309
                     /locus_tag="AC04VQ_01625"
     CDS             8488..9309
                     /locus_tag="AC04VQ_01625"
                     /inference="COORDINATES: protein motif:HMM:NF039826.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HIO9552937.1"
                     /translation="MFGFITPNFQTLSDEEKSRYQSVYCGLCHSLRDNYSNVSRLALS
                     YDLTFLVLLLSSLYEADEEKENCNCIFHPGDKYETAQNEFSDYCADLTVALAYHKILD
                     DINDEDKLIAKVGERALASQYKKVKEKLPDTCVLIEKSMAEIQKIECGPRGNRVNATG
                     KSSVGPGELFGYMLGYLFDVKHDNWSETLVKFGANLGRLIYYMDAAVDFEDDVKNDSF
                     NPFKESIKVDKIDEATAHDIKDKLTVLAGQATTHFEKLPLEQDINLLNNILYEGL"
     gene            complement(9421..10929)
                     /locus_tag="AC04VQ_01630"
     CDS             complement(9421..10929)
                     /locus_tag="AC04VQ_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552938.1"
                     /translation="MTGYTVIAGKDIVLDPVFTKEGTMSVSAVVKDKESGQPINKAFG
                     YLLGFNLSTQEVYVSKSQETGADGKFVFEDIPSGFQGVVIAGAAEHTSQMLYLSALYP
                     RQEFNLNPGVLCTVDESDNFGGVVVKAGSSIKFTDIEDEFVFPFDIDLGNDLPKMGFP
                     NTDETSKLVFDNKGNINYSFVHDPSTHNINKMNLNLKVYGKEGYAFKNWLKDGEEFTG
                     ELKLINQKTDFTIKPCFKIAGPYTFSTYIQNRKKERITNAYAYLVGVHELTGEAYLSD
                     PVTTDSEGVFAIENIPYDVSGLVVVGAPGYVNRFDYCDCDVEATFFTLDEGVQIDFQM
                     PETANTVYIEKGSGVKLTVEGIPLEFALPYDVEFGSFFLPFGITPRGTFNFMDDGRVN
                     YTVLSNENDIVINSLSVTFRVEPKDGYVFAGWLKDGQAISGEYTLKDGETDFSITPVF
                     VVPSPQPTPVDPTSPMAKTFDSIGDVFEAFAILTLAFALVYAYKTLRRRKHN"
     gene            complement(10957..11895)
                     /locus_tag="AC04VQ_01635"
     CDS             complement(10957..11895)
                     /locus_tag="AC04VQ_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552939.1"
                     /translation="MKSIKSTKLCSWLIERKLMLFIIAVSFVAMVLPGAFVCATELAT
                     DEASQPALPAAEEQATPAAETEAIDEGVVIVTGEEEATTEEALPATEAEASDEGVVIV
                     TGDDSEATPEETSETEVIVEQENGTVTGTVKLSTGSLEGKDAYVQIAGVYTKLILPEV
                     YSERVNVNADGTFTINDVPDGLSGIITAGAKGYSNQILAYPGKTENAPINFTLDVGST
                     CTFTKDKTKLGSYYLHDGSYFKPLNGDKIEYPSLIGSSISLGTGMPDFGLPAGATITE
                     NLLLGTITYHYENADKSEIHHIILSAGPEKGYDFNY"
     gene            12001..12780
                     /locus_tag="AC04VQ_01640"
     CDS             12001..12780
                     /locus_tag="AC04VQ_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HIO9552940.1"
                     /translation="MAMDAYKILGLESTASTDEVKKAYRKLARENHPDLHPGDEAAAK
                     RMNEINEAYDRIMNPDKYAAEDARRAAADQAAGRSSSGPQRPGQGPNAQQQNAGYYQQ
                     QGRYGWSGDFGFDDIFGGFYTVGGPIHPEASPSDPEEVRMAIDMINNKQFKEAIKVLY
                     RTKSTDRNARWYYIVAVANHGAGNTMLAMDQIKRACEMEPNNQEYVSAARNIQASGRV
                     YQETGDARGFSMNYMNPGTLCCMCLMLQMCMGGGIPLCIGF"
     gene            12856..14625
                     /gene="dnaK"
                     /locus_tag="AC04VQ_01645"
     CDS             12856..14625
                     /gene="dnaK"
                     /locus_tag="AC04VQ_01645"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIO9552941.1"
                     /translation="MSKSIGIDLGTTNSCVATIEGDKPVVITNAEGKRTLPSVVAFTK
                     DGERLVGEPAVRQAVMNADRTFASVKRHMGSDWRASIDGKDFTPQEISAMILRKLKND
                     ARDYLDDEKLVDAVITVPAYFNDMQRQATKDAGRIAGLNVLRIINEPTAAALAYGLDN
                     EMQQKVMVYDLGGGTFDVSIIDISEGVIEVMSTSGNNHLGGDDFDECIANHVAQVCKS
                     DYKFDVKSDPEAWARVLDAAEKAKIELSAAKKAEINLPFLSQTGNNGGPINLVCEITV
                     EDFNKMIAPLIEQTAEPVEQALYDAGIAASELDQVLLVGGSTRIPAVAAKVKELTGKE
                     PSSSINPDECVAQGAAIQANTLSNAANSLAVRGGEILLLDVCPLSLAIETVGGVATRL
                     IERNTTLPTTYSQIFTTAAAFQTKVEIKVLQGERPMAADNKMIGNFMLTGIKRAPAGV
                     PQIEVTFDIDANGIMNVKAKDLGTGKEQSITIENDQRMSDAEIEAAIRDAETYAEQDK
                     ARKQNWEDYAEAQSLLSQVDTAIAKKGKTMDKTEKKNIKTQANVLRNYLRKKQDKLTD
                     GDRVNIKDSAAKLKEMASSIIED"
     gene            14655..15089
                     /locus_tag="AC04VQ_01650"
     CDS             14655..15089
                     /locus_tag="AC04VQ_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552942.1"
                     /translation="MPKAVKNINITKKILFALAICIVVMLCFLFVAIQTASAGNRLQD
                     SSSFGIGNNRFSIDAQVGVIANGEFEGSEHFDSENTSLATYSTRDISTAISAISFRIE
                     SAAKAAAEAAKAEEMVKANIAKNRRNEHARAFGMPSGLSEVN"
     gene            15181..15465
                     /locus_tag="AC04VQ_01655"
     CDS             15181..15465
                     /locus_tag="AC04VQ_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552943.1"
                     /translation="MPTPLEKTALTQDGPLQFLTPKAARDFTALGLIMHEAEEIPAEE
                     AEKMQFTAMYQACHQAMATQFQHPMQKNTVHQIRTIDMQTLLARCAKFNL"
     gene            complement(15455..16744)
                     /locus_tag="AC04VQ_01660"
                     /pseudo
     CDS             complement(15455..16744)
                     /locus_tag="AC04VQ_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013431.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
     gene            complement(16754..>17451)
                     /locus_tag="AC04VQ_01665"
     CDS             complement(16754..>17451)
                     /locus_tag="AC04VQ_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIO9552944.1"
                     /translation="FPGNVDFYVHYEVFDDNSLHIYYSAETDKATPFNVTNHAYFNLS
                     GDHSQTVLNDELFINSLYMTLLNEDTCPTGEIVPIKRKSPFDFYGKCLFGPRRFKQGK
                     VVGEDINANDPQIKLGNGYDHNFIIQNMEKSIKAKLHPYNNQVNYCAKVHNNESGITM
                     EVFTTEPGVQLYDSVDLDGSLIGKNGVPVSSNCGLCLETQHFPNSPNIEKFPSTILKP
                     GDTFNSITIYKFS"
BASE COUNT         5169 a         3612 c         3537 g         5033 t
ORIGIN      
        1 gatttactcg tggcatttac tcgatttccc cggggccagt acttgattta ctcgtggcat
       61 ttactcgatt ttgatttttg tggtaaaata gcagtatgtt tgatgttgga aatacgcctt
      121 tagtggagtt cgaaaagatt cgcgaaaacg agaacctggc ctgccatatc ttcggcaaga
      181 tggagtacaa aaatccagct gggtcaacca aagatagaat tgttaagcaa attttggatg
      241 atgcaattgc tgatggacga cttcgcgatg gcgacgaaat tgttgaagct acttcgggaa
      301 ataccgggat tgctcttgct gcatatggcg ggatgcttgg acacgcggtt aaaattttca
      361 tgccggaaaa tatgtctcag cagcgcatag atttgatgcg ccattatggt gccgaagtgg
      421 tgctgacccc tgcttcggag aatgtcgaag gttcaattgc ggctgttcac gaatacattg
      481 acacgcacga gcactgcttt ttggctgatc agttcaataa tttgtcaaat acaactgcac
      541 acttcgaaaa caccgcgcgc gagatttggg atcaaacgaa cggcgaagtt gacgccttca
      601 ttgcaggcat tggaactggt ggaacgctaa agggtgttgg agattttctg aaagagaaga
      661 accccaatat ttatcttgcg gcggttttgc ctgctgaaag ccatcatgtc attcaaggaa
      721 ttggcgatgg cttcgacccg ccgtttttca aaaacaatga aaacattaac gaagttatca
      781 aaatcacaaa tgccgaggca atgcaatact tcaattgaat taataactcc gaagctcagt
      841 ccgccgggat ttcatctggc gcgaatcttt gcgcagcgct caaacttgcg aaagcccagc
      901 cgcagcttcg caacattgta ataatgtgcg cggacggaat cgaccgttac aattaaaatc
      961 gagtaaatgc cacgagtaaa tcaagtcctg gccccgggga aatcgagtat tagagtaatg
     1021 gggtgccggt catttctttt ggttgattta tactaattag atgaagtagt gttggagcga
     1081 tgtcgcagag ggcgccatct tcggcagtaa gttcgcggtt tttgcctgtg aagtcaagaa
     1141 ggacgaaggg aacttttgcg gtagtgtgag cggtcatggg ttcgccgtta tcttgaatca
     1201 tgcaatcggc gttgccatga tcggcagtga taagaactgc tccacccttg gcttcgatgg
     1261 ccgaaactac ttcgccaaca ccagcatcaa cagcctcaac tgccttaaca gcagcatcaa
     1321 aaactccagt gtgacctacc atatcgcaat ttgcaaaatt tacaatgtag acatctgctg
     1381 tgtctttcgc gatggcatca cttaaggttg acacaacttg cggagcagac atttctggct
     1441 gcaaatcata ggttgcaact tttgggctat caatcaactt gcgaatttca tttttcttgg
     1501 gctcttcaac tccaccgttt aggaaaaatg taacatgagc atatttctca gtttcggcag
     1561 tgtgatactg gaccagacct tcgtcagcta aaacatccgc caaaacattg ctaggaattt
     1621 cttttggaaa cgcaattggg agagtgaact tttcatcata ttcagtcaaa caaacataat
     1681 gaacattcgg gcggcccgtg atgtcgaagt ccgaaaaatc cgnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnaaacaaac ataatgaaca ttcgggcggc ccgtgatgtc
     1861 gaagtccgaa aaatccgcat cggtaagcgc atgagtaatt tgacgagcgc ggtccggacg
     1921 gaagttgaag aaaacaacgc tgtcgccatc cttcatgcca cggttaataa gcgcagttgg
     1981 gacaatgaat tcgtcagtaa catcaaccga ataagacttc tcacaaacac gagcagcgct
     2041 aagcgtttct tcgcaaggtt gagccttcac aattgcgtca taaccttttt ggacacggtc
     2101 ccagcgcttg tctcgatcca tgacataata gcgaccttga atgcttgcga tagcaatcga
     2161 agtcttcttg ccatccgcaa cgcactgcgc atttttgatc gcaattttat cttccagctg
     2221 gccgatgtat tcaacaccac ttgttggcgg cacatcgcga ccatccataa aacagtggac
     2281 gaagacatgc ggcacctctt gcgcaattgc aagatcgaac aaagcgaaca agtgttcgag
     2341 acttgaatgg acgccgccat cgctaataag acccatcaaa tgaagggcgc cgttattttc
     2401 ttttgcatgc ttgcaagccg tgataagaac ttcgttattt aaaattgaac catcctggca
     2461 agctttattg attcgcgtga gttcctgata cactactcta ccagcaccga tgttaaggtg
     2521 accaacttcg ctgttaccca tctgaccagc aggcaggccc acctcttcgc cgcttgcgtc
     2581 aagcttcgta actttgtgct cgctaaaaat tttgtctaaa ttaggagtct tcgccgaagc
     2641 aatagcgttc ttaggccccg catccgcaag accaaaacca tccataatta tcaagcatat
     2701 tggcttatcc aagttcatct tctaaccttt caaaaaggtg taattattgc caggcaaaaa
     2761 ttacaccttt tataaacacg ctttgcaaag ggcgatgaaa ctgtcggcct taagcgccgc
     2821 tccaccaatt aagccaccgt cgatgttctc atttgcgagg aggccttcgg cgttttccgg
     2881 tttcatggaa ccaccataaa gaacgcgaat gttttctgca acacgctcat cgtagatgtt
     2941 tttgatctca tcgcgaacgg cagcgcaaac ttcttcggct tgttctggag tagcagttct
     3001 accagttcca atagcccaaa ttggctcata agcaacgatg caatcctcgg catcggtcgc
     3061 atcgattccg gcaaaagcag cctgaacttg cttgcggacg aactcatcgt aagtgccttc
     3121 gtcgcgaaca ttcaaacttt caccaacaca aattattggt gtgatgtcag cggcaatgag
     3181 agccttcgcc tttttgttga tcatctcatt ggtctctccg aagatctcgc ggcgttcact
     3241 atgaccgatg atgcaatgac tgcatgggat ttccttcaac attgggacgc tgatttcccc
     3301 agtaaaagcg ccactttctt cgaagtagca attctgcgca ccaacgccaa tgtttttctt
     3361 gtcaaagttg aaaacctcaa caactggctt taggtcaaca aaaggagtgc aagtgataac
     3421 ctcaaccttc tctgcccaat cattttcata ttcgttcgaa atctcctgag caagactaac
     3481 agcttcgcca atagtcttgt tcatcttcca atttcctgcc attactaatt ttctcataat
     3541 ttctcctttt aacttttact gcctcgctgc cttccgctcg atactttcga ggaatttttc
     3601 ttcgaaaaat ttcgaaaata tctcgctggg cgcgctcggc cttaatttaa aaactactta
     3661 gtcttcccat aagcatcata aattctgttt acttctattt cgtattgcat gcgctgaccc
     3721 ttaacgaccg aactcaaaat tcagccagtt atcaggttga gcaatgcaaa gagcataaaa
     3781 cctaaggcca agattgccgt tgggaagcgc tcgactaagc ctgtattaac atagtcaact
     3841 attacaggta taccacatat aagtcctaga gcaaaacaaa ttaatgctat aatgccaaat
     3901 aacattaaag gtctatattc tttaaacaat gaaagaatca ttgttaaaac tttcatgcca
     3961 tcgccaaaag ttgagagttt gctctcggac ccttcgggtc tatctcgata atcgattggg
     4021 acttcgacga ttcgccaact cttgtccact gcatggatgg atagctcagt ctcaatttga
     4081 aaaccagggc taagaatagg aagcgccttc gcaaatactg tgttgtaagc gcgatacccg
     4141 gtcataacat cgttgaagtc aaacttataa atcatcttaa ttagccagcg taccaggtta
     4201 tttccaagcc cgtggaaggc gcggtcattc tcatcgccat aacttccgtt actgagtcta
     4261 tcgccaatgg tcatatcggc gaggttctcg cgcagtggcg cgaggaggtc atgcacggct
     4321 tcggcgggat aagtatcatc gccatcgacc attacataat aatcggccgt gatgtcacga
     4381 gtcatttggc gcacaacacg acccttacct tggcgagtct cggttcgaac aatggcgcca
     4441 tgttccttcg caatttttgc agtatcatca cttgaattat tgtcataaac gtaaatgtct
     4501 gcatttggaa gttcgcgctt gaaatcgtca acaaccttac caattgtaat tgcctcgtta
     4561 taacaaggaa ttattactgc aattttttcg tttgtgtcac tcatttaatc tccaatgttt
     4621 ttcaaattac attataaaca attttaacaa aaaatcgaga aaatgccacg agtaaatcga
     4681 gtactggccc cggggaaatc gagtaaatgc cacgagtaaa tcgagtgtgg gccccgggga
     4741 atatggtaaa attaggggcc gtgaaaaaat tgattgaaca gtttgtgaaa tttggaattg
     4801 ttggcgttat tgcgtttttc attgacttcg gcatttacac gctttgcacg caagtttttc
     4861 aatgacacta tattgttggt ggaacgctcg gcttcacaat ttcggtcatc ttcaactatc
     4921 ttgcaagcat gaaatatgtc ttcaagcaca aagaagacat gagccgcact cgcgaattta
     4981 tcatttttgt tgttctttca ataattggcc ttggcatcaa taatgcctgc ttatggttct
     5041 taattgactt cattcagtgg ccttttgagc tatcgaagcc ggtccttgaa cttggtgcga
     5101 aaatcatcgc aacggcagtt gttatggtct gaaacttcgt tactcgaaaa atccttttag
     5161 acgacaccaa aaataattaa aaaaatttca aaaagttggt gcatatatcc agcaaaagaa
     5221 taaaatatgc gcttgccgat aaatgaaaat ttatctagga ggtaaaaatg acagttaaag
     5281 ttggtattaa cggcttcggt cgaattggtc gcatggtcta tcgtgcgatg gctggtgaca
     5341 agcgttttga cgttgtagct attaatgatc ttggtaacgt cgaagtaatg gctcacctgc
     5401 ttaaatatga ctcaatccat ggtgttgctt tcaaaaaagt tgaagctaag ggtgacacaa
     5461 ttgttgctga cggtcatgag attaaagttc ttagcgaacg tgacccgaag gatcttccat
     5521 ggggcaaact tggtgttgat gttgtaattg aaagtactgg acttttcact gatggcgaaa
     5581 aagctgctgc tcatattaag gctggcgcta agaaagttat tatttctgca cctgcaacaa
     5641 atgttgatgg caccttcgtt atgggtgtta acgatgacac ttatgacaaa gaaaaacatc
     5701 acattgtttc aaatgcttct tgcacaacaa actgccttgc tcctttcaca aaagttttgc
     5761 ttgaaaactt tggaattaag cgtggtatga tgaatacgat ccattcattt acaggggatc
     5821 aggttgtttt ggatggccca cataaggatc ttcgtcgtgc aagagcttcg gcactaaata
     5881 ttattccaac atctactggt gctgctcgtg ctattggact tgttcttcca gagcttcaag
     5941 gcaagttgga cggttttgca actcgcgttc ctacgcctga tggatctttg gttgacttaa
     6001 ctgcagaact tgaagttcct gttacaaaag aacaaatcaa cgaggtcatg aaaaaagctg
     6061 caaatggtcc gcttaaggga atcttggaat acgccgaaga ccctcttgtt tcttcggaca
     6121 ttattgataa ccctcattca tcaatttttg attctaaact tacaatggtt atgcctggtg
     6181 gagttgtttc aaaagttgta gctggcgaag acgcaggaac aatggtcaaa tgcgttgcat
     6241 ggtatgacaa cgaatggggc tacagtaatc gcgctgctga ccttgcagca atcatgctct
     6301 aagtagcatt ttatatttgg ggaaaaaagc cggcctagac gtcggctttt tttatgatgc
     6361 gacgtaaact cgaagagctt ttgagtgagt ttttatatca aaattatggt tcgaatattc
     6421 ttcgccatca agctgacaag gaacaagttg attgattttt agtttgatat ttttagattt
     6481 atcaaaagca acgtgtttgc ttccaacgtg cttacctttc gccgcacgtg cgaaaagcgg
     6541 caaggtagtt tttcgagtca aatttccaac tgaataacac atattaaaat agccatcatc
     6601 aagttcggca tccggcgtga ttttaaaacc tccaccatat gttttaccaa tttgaattgc
     6661 gaaaattatc gaatggccat tgatcttgcg gcctcggtcc agcttcgctt cgaaatcaaa
     6721 aaattcgaag tgattcataa ttctgtcaac gccgcatttg aaataaagct taaagccttt
     6781 ttccttgctc ttctgacgag ccttcattgt gtcaattgca attccggcat caagaccaaa
     6841 tgacagggtt tcaacaaaat atttatcatt gcattggcca acatcaactt gcttgacctt
     6901 cgcatgcatt aactggtcca cggcatggtg aacattaaag gacatgccaa ggcttcgtgc
     6961 gtaatcgttt ccagaaccaa caggaataac tcccataatt ggacgctttg cttttggaat
     7021 tctcatgatc cccgaagctg tttcgtgaac gacgccatcg ccgccaacaa caattactga
     7081 atcgtatttg gctgtgtctt cggcaatttt cattgcgtga ccatcgtgtt tggtgtagaa
     7141 caagtcaacg tcttggtgat attcggcacg caaagtttcg aaagttttgt agccgttttt
     7201 gctgcccttt ccactttgag caacagggtt aacaataatt aaagctcttc ctaagttgtt
     7261 ggccatggca tctcctacaa tttcgaacga tcaaccttag gataaggctt gccacacgac
     7321 atgtcgcctt cgatgcattt tgtgcgaacg cattcaggac cggcatccgc aaaaataagt
     7381 ggagcagttg gacgaacaag ctcaagcatt tgccaagcaa ggtcgttgat ttctcattga
     7441 gctctgttac aacaacgaac gctgaagaag tgaagcagct cgcgaatgtt catagttaca
     7501 acaatttttg tctctgccgc atttggcaaa atgaaacgag cgtcttcctt cggcacacca
     7561 gcagcaacca acttctcata agtttcctca caagatttaa tggcttcgtc aaagatttgg
     7621 ttagcctcgg cgttgtcttt aacggtctga ggcttgacga agctgaggcc atccttaacc
     7681 gcaacataac gctgactttg ctggttgtag ctggccatac ggtgacgaac gagttgatgt
     7741 gtaagcgcgc gagacacgcc ttcgatagca aatgtataac ttgcatgctc cagagttgag
     7801 ctgtggcctc ccgctaaaat aatttttagg atttttgcaa cttgttcatc tgtcattttt
     7861 tcgattaaat cggccgcccc aacagcacta taacaaagac gcgccgcagt tgctacagca
     7921 cgctcgggat ctttagtgtg atataaaagt ttaacattga tttttgtttc cattttgact
     7981 ccataacttg gcttgcgaca ttttatgata ccaaaattta ccgcgcacaa acccatataa
     8041 tgttataatc taacgcccaa tttattaagg ttagtatgag agaatttttt gacagactaa
     8101 acattcgaat tgctgaattc atggaaggcc gatatgggct ggataactta aacaagtttt
     8161 tgctcatctt cggttttgtt ttggttttct ttggtgcgat tgtttcccct gttgatttac
     8221 tgggatgggg atttgcaatt attgctttgt ttcgtgcgct gtcaagaaat tttgacagac
     8281 gccaaattga aaacgaatgg tatctaaaag ttactcgcgc gccgaagcga tttttcaaga
     8341 aaatttcaac gcgttggaaa aatcgcaaaa ctactgtcta tttcaaatgc agcaattgta
     8401 agcaaccgct ttctgttcca aagggcaagg gtaagattcg cgtaatttgc ccaaaatgcg
     8461 gaaccgaaat ttataaaact acttaaaatg tttggtttta tcactccaaa ttttcaaact
     8521 ttaagcgacg aagaaaagtc ccgctaccag tccgtttatt gcgggctttg tcatagtttg
     8581 cgtgacaatt attcgaacgt ttcgcgcctg gctcttagtt atgatttgac gtttttggtt
     8641 ttgcttctaa gttcgcttta tgaagctgac gaagaaaagg aaaactgcaa ttgcattttt
     8701 catccgggag acaaatacga aactgcgcaa aatgaatttt cagactattg cgctgaccta
     8761 acagttgcac ttgcctatca caaaattttg gatgacatca atgacgaaga taaactaatt
     8821 gcaaaagttg gagagcgcgc cttggcttcg caatataaaa aagtaaagga aaaattgcct
     8881 gacacttgcg ttttgattga gaagtccatg gctgaaattc aaaaaatcga gtgtgggccc
     8941 cggggaaatc gagtaaatgc cacgggcaaa tcgagtgtgg gccccgggga attgtttggt
     9001 tatatgcttg gttacttgtt tgacgttaag catgataatt ggagtgaaac gttggtgaag
     9061 tttggggcga acctcggacg cttaatttat tacatggatg cagcggttga cttcgaagat
     9121 gacgtgaaga atgattcgtt taatccattt aaagaaagca ttaaagttga caaaatcgac
     9181 gaagccacag cacacgacat taaagataaa ctcacagttt tagcaggtca agcaacaacc
     9241 cacttcgaaa agctacctct tgagcaagat attaaccttc taaataatat tctctacgaa
     9301 ggcctttgaa ttcaattcaa ccataaattc ttcaaaaaga tgtaattcat gcctggcaat
     9361 aattacacct tttggtgcat ttcatgcctg gcaataatta caccttttta aggttgttaa
     9421 ttagttatgc tttctgcggc ggagagtttt atatgcatat accaacgcga aagctaaagt
     9481 caaaattgcg aaggcctcaa aaacatctcc aatgctatca aatgtttttg ccattggcga
     9541 agttggatca acaggagttg gttgaggact aggaactacg aaaactggag taattgaaaa
     9601 gtctgtttcg ccatctttaa gtgtatactc gccggaaata gcttgaccat cctttagcca
     9661 tccagcaaat acatagccat ccttaggctc aactctaaaa gtaacactta atgaattaat
     9721 cacaatatca ttttcattac tcaacacagt ataatttacc cttccatcat ccataaaatt
     9781 aaaggtacct cttggcgtaa ttccaaatgg aaggaaaaag ctaccaaatt ccacatcata
     9841 tggcaatgca aattcaagtg gaattccttc aacagttaat ttaacacccg atcccttttc
     9901 aatataaaca gtgtttgcag tttcaggcat ttggaaatca atttgaacac cctcatcaag
     9961 agtaaagaat gttgcttcaa catcgcaatc acaataatca aaacgattaa catagcctgg
    10021 tgctccaacg acaactaaac cactgacatc atatggaata ttttcaatag caaatactcc
    10081 ttcgctatca gttgtaacag gatcacttaa atatgcctca ccagttaatt cgtgcacgcc
    10141 aacaagataa gcatatgcat tagttattcg ctctttttta cgattctgaa tgtaggtgct
    10201 aaatgtataa ggaccagcaa ttttaaaaca aggcttaata gtaaaatccg ttttttgatt
    10261 tattagcttt aactcacctg taaattcttc accatcctta agccaatttt taaaagcata
    10321 accttctttg ccatatacct ttagattcaa attcattttg tttatattat gagttgaagg
    10381 atcatgaaca aatgaataat ttatatttcc cttattatca aaaacaagtt tcgaagtctc
    10441 atcagtatta gggaagccca tctttggtaa atcatttcct aaatcaatat caaaaggaaa
    10501 tacaaattca tcttcaatat cagtaaattt aatcgaagag cctgctttaa caacaactcc
    10561 accaaaatta tcagattcat cgacagtaca taatacacca ggatttaagt taaattcctg
    10621 acgaggataa agagcactaa ggtaaagcat ttgcgatgta tgttcagctg ctccagctat
    10681 tacaactcct tgaaaaccgc ttggaatatc ttcaaaaaca aacttaccat cagcacccgt
    10741 ctcttgggat ttgctaacat agacttcttg tgttgataaa ttaaaaccta ataaataacc
    10801 aaaagcctta ttaataggtt ggccagattc cttatcttta acaactgcac taacggacat
    10861 tgttccctct ttagtaaaaa caggatcaag tacgatatct ttaccagcta tgactgtata
    10921 acctgtcacc tctggaataa cctcaccatt aatagttcag taattaaagt catatccttt
    10981 ttctggacca gctgataaaa taatatgatg aatttcagat ttatcagcat tttcataatg
    11041 ataagttatg gttcctaaaa gaagattttc agtaatcgtc gcaccagcag gaagaccaaa
    11101 atcaggcatg ccagtaccta aactaatact tgaaccaata agagatgggt actcgatttt
    11161 gtctccattt aatggtttga aataagaacc atcatgaaga taataactac caagttttgt
    11221 tttatcttta gtaaatgtac aagtagaacc tacatctaat gtaaaattaa taggggcatt
    11281 ttcagttttt ccaggatatg ccaaaatttg attggaataa cctttcgctc cagctgtaat
    11341 aattcctgat agtccatcag gaacatcatt aatagtaaat gtgccatctg cattaacatt
    11401 tactctctcg gaataaactt ctggaagtat taacttagta tatacacctg caatttggac
    11461 atatgcatct ttaccttcaa gtgaaccagt cgatagttta actgtaccag taactgttcc
    11521 attctcttgc tcaacaataa cttcagtttc ggaggtttcc tcaggcgttg cttctgagtc
    11581 atcgcctgtg acaattacaa cgccttcatc agaagcttca gcttcagttg caggaagagc
    11641 ttcttcggtt gttgcttctt cttcgccagt aacaattaca acaccttcgt cgattgcttc
    11701 ggtttcggct gcaggggttg cctgttcttc ggcagctgga agtgcaggtt gtgaagcttc
    11761 atcagttgca agttctgttg cacaaacaaa tgctcctggt aacaccattg caacaaaaga
    11821 aacagcaatg ataaatagca ttagtttacg ctcaattagc caactgcata gtttggtaga
    11881 tttaatagat ttcatttttc ttccaatctc taaatttatg tggattcgaa ttagcgtggg
    11941 aaggtcatta tacaacataa attgccaaaa aattagaaat tttgatagaa tatagcgcct
    12001 atggctatgg acgcttacaa aatattagga cttgaaagca cggcttcgac tgatgaggtc
    12061 aagaaggcgt atcgcaagtt ggctcgcgag aatcatccgg atttgcaccc gggagacgaa
    12121 gctgcagcta aacgtatgaa tgaaatcaac gaagcttacg accgcatcat gaatcctgat
    12181 aaatatgctg ccgaagacgc gcgacgagct gcggctgatc aggctgctgg acgttcttca
    12241 tcaggtccac aacgcccagg tcaaggtccg aatgcgcaac aacaaaatgc tggctattac
    12301 caacaacaag gtagatatgg atggtcggga gactttggct tcgatgatat ttttggaggc
    12361 ttctacacag taggtggacc aattcacccg gaggcctcgc cttcggaccc tgaagaagtg
    12421 agaatggcca ttgacatgat caacaacaag caatttaagg aagctattaa ggttttgtat
    12481 cgcacaaaaa gcacggatag aaatgcacga tggtattaca ttgttgcagt tgcgaaccat
    12541 ggagcaggca atacgatgct tgcgatggat caaattaagc gcgcttgcga aatggagccg
    12601 aataatcaag aatatgtttc ggcggcgcgt aacattcaag caagtggtcg agtttatcaa
    12661 gagactgggg acgctcgcgg atttagtatg aattatatga acccgggcac actttgttgt
    12721 atgtgtttaa tgcttcaaat gtgtatgggc ggcggcatcc ccttatgtat aggtttttag
    12781 ttttcaccag ggaaatcgag taaatgccac gagtaaatcg agtgtgggcc ccggggaaat
    12841 cgagaaggag tggagatgag taagagtatt ggaattgatt tgggtacaac taatagttgt
    12901 gttgcaacta tcgaaggtga caagcctgtt gtaattacaa acgccgaagg caagcgcacg
    12961 cttccaagtg ttgtggcttt tacaaaagat ggcgaaagac ttgttggtga accagcagtt
    13021 cgtcaagctg ttatgaatgc ggatcgaacc tttgcttcgg taaagaggca tatgggaagt
    13081 gactggcgag cttcgattga cggcaaggat tttactcctc aagaaatttc tgccatgatt
    13141 ttacgcaagc ttaaaaatga tgcgcgtgat tatttggatg atgaaaaatt ggtcgatgcc
    13201 gtaattacgg ttccagctta ctttaatgat atgcaacgcc aggcaacaaa agatgccgga
    13261 cgaattgcag gcttgaatgt gcttcgtatt attaatgagc caaccgctgc tgcccttgct
    13321 tatggtcttg ataatgaaat gcaacaaaaa gttatggttt atgaccttgg tggtggaact
    13381 tttgatgttt caattattga tatcagcgaa ggtgtcattg aagttatgtc caccagcgga
    13441 aataaccacc ttggtggcga tgattttgat gaatgcattg ccaatcatgt tgctcaagtt
    13501 tgcaaaagcg attataaatt tgatgttaaa agtgaccccg aagcttgggc acgcgtcttg
    13561 gatgctgccg aaaaagcaaa gattgaactt tctgctgcga aaaaagccga aatcaatttg
    13621 ccgtttttga gtcagaccgg aaataatggc ggacctatta atttagtttg cgaaattaca
    13681 gttgaagatt tcaacaaaat gattgctccg cttattgagc aaactgccga accagttgag
    13741 caagcgctat acgatgctgg aattgcggca agcgaacttg atcaagtttt gttagttggt
    13801 ggaagcacac gaatccctgc tgttgctgca aaagttaaag agttaacagg caaagagccg
    13861 tcttcgtcga ttaatcctga cgaatgtgtt gctcaaggag ctgcaattca agctaacact
    13921 ttaagcaacg cagcaaattc tttagcggtg cgaggcggcg aaattctgtt gcttgacgtt
    13981 tgtccgctat cacttgcaat tgaaacagtt ggcggagttg caaccagact aatcgagcgt
    14041 aatacaactt tgcctacaac ctattcacaa atttttacga cagctgcagc ctttcaaaca
    14101 aaggttgaaa ttaaagtttt gcaaggcgag cgcccaatgg ctgccgacaa caaaatgatt
    14161 ggcaacttca tgttaactgg aatcaaacgt gctcctgcag gtgttcctca aatcgaagtt
    14221 acatttgaca ttgacgcaaa cggaattatg aatgttaagg caaaagattt gggaaccgga
    14281 aaagagcaat caatcacaat tgaaaatgat cagcgaatga gtgatgctga aatcgaagct
    14341 gcaattcgcg atgctgaaac atacgccgaa caagacaaag cgcgtaagca aaattgggaa
    14401 gattacgccg aagcgcaaag tttgctttcg caagttgata ctgccattgc caaaaagggc
    14461 aaaactatgg acaaaactga aaagaaaaac atcaaaacac aggccaatgt tcttcgtaac
    14521 tatcttcgta aaaaacaaga caaattgacc gatggtgacc gcgtaaatat taaagattcc
    14581 gctgcaaaac ttaaggaaat ggcttcgtcg attattgagg attaaccaag aatttttgct
    14641 ataatactca acccatgcct aaggctgtta aaaatataaa cattacgaag aaaattctct
    14701 tcgcattggc aatttgcatt gttgttatgc tttgcttttt gtttgttgct attcaaacgg
    14761 caagcgcagg caacagattg caagattctt cgagttttgg aattggaaat aaccgattta
    14821 gcattgatgc gcaagttgga gttattgcaa atggtgaatt tgaaggcagc gaacacttcg
    14881 attcagaaaa tacttcgctt gcaacttatt caacgcgaga catctcgact gcaatttctg
    14941 caatttcatt taggatcgaa tctgctgcta aagctgctgc cgaagctgca aaagccgaag
    15001 agatggtcaa ggccaacata gcaaagaacc gaagaaatga acacgctcgc gcgtttggca
    15061 tgccttcggg cttaagcgaa gtaaattgag atgttggtcg catggcattt gtcgctgaat
    15121 ggacagtccg cattaatgca tattttattg gaactccatt ggcagacaca ggtgagtttt
    15181 ttgccaacgc cgcttgagaa aacggcattg acccaagatg gtcccctgca atttctaaca
    15241 ccgaaagcgg caagggattt cactgcttta ggtctcataa tgcatgaggc tgaggagatt
    15301 ccggctgagg aagctgagaa gatgcaattt acagccatgt atcaggcctg tcatcaagct
    15361 atggctacac aatttcaaca tcctatgcag aaaaatactg tccaccaaat tcggacaatt
    15421 gatatgcaaa cactcttagc gagatgtgca aaatttaatc tatagtaaat ctatattcag
    15481 attttgagtg aaaaacttct ccaggcaata atattgtatt aggaaaattc ggctgatttg
    15541 gactgtctgg aaaatgctga ggctccaatg ccattgctgc atattttgga agaggcttat
    15601 tgttctttcc aatttcagaa ccagtataat catgacagtc aaaaagctgg attgccggtt
    15661 cagttgtaac aattgtcatt gtaattccgc tcttattact tttaactttt gcagcaagct
    15721 tcgccttatc atttaatgcc gaaacaccat cgtctgtaat ttcaaacttt ccatcaaatg
    15781 gtgcaccttt tgcagctgct tcttctctac tcattaaaac gaagttatgg tcataacctt
    15841 tcccattttt tagctgttcg tcttcagcgt taatgtcacg ccctacaagt ttaccttcgc
    15901 cagcttctcc aaagaaatca aatacaccac ctttaggtgt ttgacgaatc tcaccagttg
    15961 gaataagctc agcatcaata ggggttgttg cattactata aattgttaaa ttttcttcag
    16021 ttataggcca acttgggtct ccagaaacgc aaaagaaaca atgattcatt agattaacag
    16081 gacacggcat tgtagtcttc gcttcataag caattccaag tgtgttgttt tcgttcaatg
    16141 tgtaagtaac aattgttgtc atgcgaccag gaaaacccat atctccatgt tcgctgtgta
    16201 aaataaattt aatgaaacga gatgggtcat attcgtcaat ttcaaatgtt tgatatgcaa
    16261 acccaaactt accgccatga agagagttat cattttcgtt aatatcaact tgatatgttg
    16321 taccattata tgttcacttg ccatctttta ttctgttaga aaaacgacca acaatcgccc
    16381 catgaaaatt tttcattgtg ttattgaaat taacatattt gcgaatgtca tcaaagccca
    16441 aaaccatatc cttaagttcg ccatttttat caggaaccat taaagaaaca actcgagctc
    16501 caaaatttgt aatacaaact tctgcgccat ttttgttaac aagtgtataa agtttagttg
    16561 gaacatcaga attatttgca tcttcaagtc caagacctaa atctcgaaca ccatttgttt
    16621 caaattttga aaaatccaaa ccagactttg ttctggcaga acatccagtt aaaacgggag
    16681 aaattccagc agcagcaact gttgcagcac cgcctaaaat aaaagatctt ctagaaatat
    16741 tcacaactcc tccttagcta aacttataaa ttgtaataga gttaaatgta tctcctggct
    16801 taagaattgt tgatgggaac ttctcaatgt ttggtgaatt tgggaaatgt tgagtttcca
    16861 aacaaagtcc acaattcgaa gaaacaggaa caccattttt accaattaga cttccatcta
    16921 aatctacact atcatatagt tgaacacctg gctcagtagt gaaaacttcc atggtaattc
    16981 cactttcatt attatggacc tttgcacaat aattaacttg attattgtag ggatgcaatt
    17041 ttgcttttat agatttttcc atattttgaa taatgaaatt atgatcatag ccattgccca
    17101 atttaatttg agggtcattt gcattaatat cttcgccaac aaccttacct tgcttaaatc
    17161 gtcttgggcc aaacaaacat tttccataaa aatcaaatgg agactttctt ttaattggaa
    17221 ctatctcacc agttgggcat gtgtcttcgt taagaagagt catataaagt gagtttatga
    17281 acaattcatc atttaaaaca gtctgagaat ggtctccact taaattgaaa tatgcatgat
    17341 tcgtaacatt aaatggagtt gctttgtcgg tctcagctga ataataaatg tgaagcgaat
    17401 tatcatcaaa gacttcataa tgaacataaa aatcaacgtt tcctggaaag c
//
LOCUS       DBNCDC010000011        16226 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_1120_length_16226_cov_7.858760, whole genome shotgun sequence.
ACCESSION   DBNCDC010000011 DBNCDC010000000
VERSION     DBNCDC010000011.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 16226)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16226)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16226
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1120_length_16226_cov_7.858760"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(100..369)
                     /locus_tag="AC04VQ_01670"
     CDS             complement(100..369)
                     /locus_tag="AC04VQ_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIO9552945.1"
                     /translation="MIKKQRIPKIDIYKCGHHGSANALYEKNAKTLSPKITLISVGAK
                     NRYGHPNSKTIQMLESAGSQIYRTDQNGTITCKFVGSQIQIKTDK"
     gene            complement(505..864)
                     /locus_tag="AC04VQ_01675"
     CDS             complement(505..864)
                     /locus_tag="AC04VQ_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIO9552946.1"
                     /translation="MLDVGQGDSILLRSGGKSFLIDTGNQDSMLKSRLAEQGISHLDG
                     VLITHPDDDHCGSLDVIDDLVVVSQFYVAKDLPTCSEGNCQKLMSQIHKIVKDEDVVK
                     LKVGDKINFGEIELEII"
     gene            complement(1105..1725)
                     /locus_tag="AC04VQ_01680"
                     /pseudo
     CDS             complement(1105..1725)
                     /locus_tag="AC04VQ_01680"
                     /inference="COORDINATES: protein motif:HMM:NF015714.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
     gene            complement(1855..2232)
                     /locus_tag="AC04VQ_01685"
     CDS             complement(1855..2232)
                     /locus_tag="AC04VQ_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552947.1"
                     /translation="MIFANNLNNNMHNILSAGEGEYVFEVEEDQKVGTYTNNCDAKIS
                     GSNYKVKLQVPQDFEEVHAGDIIKAKVKFSSPIELQTSNYWQNGLCANARASEASKLT
                     RTDPIGLIFTFRNHLITTIRNFD"
     gene            complement(2481..3008)
                     /locus_tag="AC04VQ_01690"
     CDS             complement(2481..3008)
                     /locus_tag="AC04VQ_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIO9552948.1"
                     /translation="MLLVALIIVIALFNFFSGSNSISVEASEDSFEQKQSEIKVHVAG
                     EVKKPGLYKLEEGARINDAVDAAGGFTDNADKDSLNLAKLVEDGEQIMIPAKGGAESA
                     AGSSSSPTNNGKVNINTADLTTLQTLNGVGPSTAQKIIDYRNANGKFKSIDDLKKISG
                     IGEKTFAKFKDQICV"
     gene            3360..3632
                     /locus_tag="AC04VQ_01695"
     CDS             3360..3632
                     /locus_tag="AC04VQ_01695"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HIO9552949.1"
                     /translation="MPGENNISENRIKIDEIDKKIIELLNQRADLSLTIRDIKKASGV
                     EIYDPAREEEIIDALCKINGGPLDDKSIRELFSKILEVMKKLPDKK"
     gene            3635..4435
                     /locus_tag="AC04VQ_01700"
                     /pseudo
     CDS             3635..4435
                     /locus_tag="AC04VQ_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012789.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            4421..5716
                     /locus_tag="AC04VQ_01705"
     CDS             4421..5716
                     /locus_tag="AC04VQ_01705"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIO9552950.1"
                     /translation="MLNFEKDYKHTTTVKLEQNYRSTSNILDAANNVIANNDKRKKKK
                     LYTEGEAGERIGVYVASNERDEGRWIAGEIERRRLRKVRLADMAVFYRTNAQSRVLED
                     MLIRAGIPYKIVGGVKFFERAEIKDVMAYLSLLINPHDDVSAIRVINTPKRGLGKTTV
                     DHIKQIATLDKCSFMDACVHVLNDESLKPQAQKSLGDFVSLIKKNSEAEAQGDTTGSL
                     RLRIEKYIKNTGLIDALKAEQTTEADTRIENINEFFGVCEEFSNTHDEEEMMFDAPGP
                     EDAKIIEKNSEPERTIRGDSLPDFLEWVRLRTDLDNLGAVGDADNNSKNSDDAITLMT
                     VHSAKGLEFDYVFVTGMEEGIFPHATAFGDFEETEEERRLAYVAITRARKELLLTCAQ
                     TRQLQGMTNANPISRFIGEIPNELKKSYGVGSLEYSGFG"
     gene            5873..6001
                     /locus_tag="AC04VQ_01710"
     CDS             5873..6001
                     /locus_tag="AC04VQ_01710"
                     /inference="COORDINATES: protein motif:HMM:NF044424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552951.1"
                     /translation="MQHKVFGEGKVTRVDGDRIYVKFLRSGQEKTLMKDFAPIVKI"
     gene            6080..7435
                     /locus_tag="AC04VQ_01715"
     CDS             6080..7435
                     /locus_tag="AC04VQ_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552952.1"
                     /translation="MNKKSAKSKLSLSFIGILLAALSLCFLAFTSANAVSDEPEPEIP
                     DVPQGNIMLEVQRSDLGTIQYQINGTLYPLTESKVLTAEDMKDLKEIYLLIKPNQDAI
                     IDKESTIIHNGDNFVSLKDQDYIDLEAGKYKLTYNPNAKKGQYAKLAILETRLVTVSL
                     TDASSKMLANNFTLMLGHGDKNDVKVTDIGTMNNIRIDKGTTDKLIMSFAIRSETYFI
                     PYITVNGQKYETVDTGMHDEEEHVIICSTLEGDAAKPDTFQVDLSIYARPECESRSES
                     MKIVQEATSYDYMIDGVVVNKGDYKLSGSDTPTDPQVENAVATYDLELTKDSIPETDI
                     TGGMDIHLLLKPSTYSVQDYKVVRNHDGVVEELDTTCEVVRNRETGEVEGVDVGFNSD
                     KFSDFSVVPVNSQPVVPEDTQVTAQTGDNIPYGIALVVLAVSAFAVYRTARRPKGAHV
                     K"
     gene            complement(7783..8160)
                     /locus_tag="AC04VQ_01720"
     CDS             complement(7783..8160)
                     /locus_tag="AC04VQ_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552953.1"
                     /translation="MFSLSANENTTSKVEVSNDVADNKNTVYTSMGRMSTPVYSYKDG
                     VVITPRAKSGNIYYQLTYINNKGEVQDSICLPQNMRPLEAGYGQTGFNFSFDAIYNYG
                     EGISNLGTYTPMDKTNDDYTNLK"
     gene            complement(8551..9048)
                     /locus_tag="AC04VQ_01725"
     CDS             complement(8551..9048)
                     /locus_tag="AC04VQ_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552954.1"
                     /translation="MCARKKKNTQGVVDLNQARLEENEKGRPSVAAAQKVPSPKVDKK
                     PSEKSAEKQMPTINEKNKKIKPIGNVSDFKEKKKPFFNRDENGKLLLTRRNLIYGAIG
                     AGGLLAVGTGLKVATDTISNVVNSIKTISVPKKDVVNSDNLAESEAKFLSVAREIELP
                     YGSLA"
     gene            9121..9471
                     /locus_tag="AC04VQ_01730"
     CDS             9121..9471
                     /locus_tag="AC04VQ_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552955.1"
                     /translation="MSQRNPMNERYTTEAKAGGHTRKSAASAKPKSSAAASVVMGSSK
                     KKPAKNAKNQPKSKRERKREERNKQYEAERRYGDPPTRAFKIAKRMWIGFLIASVVTV
                     ALSFVASRIEGLPE"
     gene            9472..9777
                     /locus_tag="AC04VQ_01735"
     CDS             9472..9777
                     /locus_tag="AC04VQ_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552956.1"
                     /translation="MPMAFLIAAYACIIVTLYLDLGKIRRMRKEYVAKMSASQTKESR
                     AEQKKRKAAERQAAKEAEAKRIEEEKKRAEEKAKGKSTRANMSFGGKVKSFFKVNGD"
     gene            9808..10035
                     /locus_tag="AC04VQ_01740"
                     /pseudo
     CDS             9808..10035
                     /locus_tag="AC04VQ_01740"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
     gene            10060..10425
                     /locus_tag="AC04VQ_01745"
     CDS             10060..10425
                     /locus_tag="AC04VQ_01745"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIO9552957.1"
                     /translation="MDNEGRLLALEFDKFWFVCVYTPNAQNELARITPRLKWGERFTE
                     FCKELESGAHGKGKKPVVMCGDFNVAHNEIDLKNPAANRGNAGFSDEEREDFTKLLDA
                     GFVDTFRNLYPDKTDAYSW"
     gene            complement(10571..11071)
                     /locus_tag="AC04VQ_01750"
     CDS             complement(10571..11071)
                     /locus_tag="AC04VQ_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552958.1"
                     /translation="MFEENMTKKTEIKKQRTAVKGLSIATISLSAFSIFVALLGVFFI
                     VLAFGLVHVEDLTLNIAGNTPLGYNTSIENENLIIGDTVNNIFDLFNFGLGLILGWTI
                     FSLLASAFSLAISIISLCALGKRKYPENIFILNIVSAVFSLISVNLIRCAVISVGSYF
                     IYKIKS"
     gene            11134..11664
                     /gene="hpt"
                     /locus_tag="AC04VQ_01755"
     CDS             11134..11664
                     /gene="hpt"
                     /locus_tag="AC04VQ_01755"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIO9552959.1"
                     /translation="MEKLGKIIFNEYEIQARVSRVADEINRDLKDKEGDGIVVVGVLN
                     GAYVFMADVVRHLNFELEVDFIQASSYKNSMQSSGYIKIEKDLTSILQDKHVLIVEDI
                     VDTGRTLTFLKKLFLDRKAKSVSICALLVKNHSEAKNLVDYKCFDCPDEFIVGYGLDY
                     RQKYRNLPYITSINTK"
     gene            11725..13869
                     /gene="ftsH"
                     /locus_tag="AC04VQ_01760"
     CDS             11725..13869
                     /gene="ftsH"
                     /locus_tag="AC04VQ_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIO9552960.1"
                     /translation="MDEKEKHTQDKREAKEQKRQDKIKKQKDKEAQLQRDIALMKKEI
                     EENKLKRGSGSGGDGQGKKPKKSTIVIIAIAVGLLLFFIGNQVFNSADSLMTDELTTS
                     EFIQAVEQDRVQNVIYNTGDYVVHGSYTPSKSIGQNSAETFNDAFKALNEGTLNILNP
                     VQTQNIEGQGAPTRNYKATFVGQDSLLELMQKHPNIQYEIVLPSGFADIFMTLLPIIA
                     IGILLFIFFSQMTKMNNQQMGFGKTKSKKSEQERPDVKFADVAGVDEAVEEMSEVKDF
                     LSNPKKYQDMGAKIPKGCLLVGPPGTGKTLLARAVAGEAGVPFFNISGSDFVEMFVGV
                     GASRVRDLFKQAKECAPSIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQILVEMDGFD
                     KNDSVVLIAATNRADILDPALLRPGRFDRQIVVDVPDVKGREKILKVHSKGKPLDKDV
                     DLWRVAQLTPGFTGADLANLMNESALLAARKNKKTISQLEVSESMERVIAGPERKSRI
                     IDDETKKTIAYHESGHALIGHLLPKADPVHKISIISRGRALGYTLSIPKEDKVLNSYG
                     EMMDELCVFMGGRVAEEIFCEDVTTGASNDLERATKMARQIVTQYGMSSELGTQVFGE
                     PNHEVFLGKDYGNTKDYSEKTAERIDEEVSKIMKNAHDRAVMILSNNKEQMDTMAEVL
                     IERETIEGEACQALLDNKWQEYVLKEKAGNLK"
     gene            13876..14346
                     /gene="smpB"
                     /locus_tag="AC04VQ_01765"
     CDS             13876..14346
                     /gene="smpB"
                     /locus_tag="AC04VQ_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIO9552961.1"
                     /translation="MSAQKQDQIVANNRKAFHDYEILDKFEAGIELTGAEVKSLREGQ
                     CQLKDSFVFIRRGEAWLYNVHISPFRNGNIANRDPERKRRLLLHKKEIRNLKQATQEK
                     GMSIIPLKVYFKAGHLVKVEIAVARGKKLHDKRQAIAKKTQMREAESYLKSRSR"
     gene            14346..14741
                     /locus_tag="AC04VQ_01770"
     CDS             14346..14741
                     /locus_tag="AC04VQ_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552962.1"
                     /translation="MAKAEEINESQLSDDVFELDFDEESVEYYIVDEDDNEIGVCLNE
                     DGQLVEYLYEDEIEAEPQIQDHSANRAVKKQVDDAAQSIKNMRDELGNASEDAKAVAQ
                     ELKDTAEELQGMLNEVKDSFKIFPSKKKK"
     gene            14901..15734
                     /gene="rpsB"
                     /locus_tag="AC04VQ_01775"
                     /pseudo
     CDS             14901..15734
                     /gene="rpsB"
                     /locus_tag="AC04VQ_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
     gene            15734..>16226
                     /gene="tsf"
                     /locus_tag="AC04VQ_01780"
     CDS             15734..>16226
                     /gene="tsf"
                     /locus_tag="AC04VQ_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015760400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIO9552963.1"
                     /translation="MAEITAAMVKDLREATGAGMMECKNALKEADGDFDKAVDVLRTK
                     GLAAVEKKAGRSTNEGTVMAIVSDDGKTGAVLELNCETDFVAMNEKFQAYAKRITTAA
                     LENKPADVDALKACEEGGETVEAILTDAIHVMGENTQIARFKVVEAPAIISYIHTGGK
                     IGVL"
BASE COUNT         5050 a         2926 c         3509 g         4741 t
ORIGIN      
        1 ggacaacttt agacttcagc ttgtcatctc caactatcgc ataaaccggc aacaaatctt
       61 tcttcaattt ctcactcata ttaattattc tatcatatac tacttatctg ttttaatttg
      121 gatttgactt cctacgaatt tgcaagtgat tgtgccgttt tggtctgtgc gatagatttg
      181 agagcctgcg ctttcgagca tttgaatggt tttgctgttt gggtggccgt agcggttttt
      241 tgctccgacg cttattagtg taatctttgg gctgagtgtt ttggcatttt tttcgtagag
      301 cgcgttggcg cttccgtgat gaccgcactt atagatgtca atctttggta tgcgttgctt
      361 ctttatcatt gaagaaattt catccttttc tgcatctccg cataatagcg agcgcgactc
      421 gacttcgcca tcattaccaa catctacgct tgctacgcaa gtgaggctgt cacaatttcc
      481 tccattatct tgatacttgt ccggtcaaat aatttccaat tcaatttctc cgaagttgat
      541 tttgtcgcca acttttagtt ttactacatc ttcatctttt acaattttat gaatctgaga
      601 cattaacttt tgacaattgc cttcactgca agttggtaaa tctttagcta cgtaaaattg
      661 gctgacgacg actaagtcgt caattacgtc aagcgaacca caatggtcat catccggatg
      721 agtaattagg actccgtcaa gatggctaat tccttgttcg gcaagcctgg attttaacat
      781 gctatcttga tttcctgtat ctatcagaaa tgattttcca cccgaacgaa gcaaaatcga
      841 atcgccttgc cccacatcaa gcatcaccaa ttctgttccg gcagcccttg gaaaaataaa
      901 gaacgctaaa accagcaata cgcaaaaagg tgtaattatt gccaggcaat atttacacct
      961 tttgggttga aaactgtcct ttatttgttt tggtcagaaa atatatagag taaaacatat
     1021 gattaaagtt atcgctatag cgacgaagat gtttagagta gcggggatgc ttgaatatgg
     1081 aattgaagat aagagcctaa cggttcaaga cataagttcg cttatacagt atgcaataaa
     1141 cataaatatt tttgaaagta tgggaagtag agtgctaaag catgaggcga aaattgcagc
     1201 tactaaacct cctccgcata aaatagtaaa gataggtgtg caaatgatat ttgatagtgg
     1261 tgccacaagt ggcagttgat taaatattgc aattgataat ggttgggaac aaatgcttgc
     1321 tgcaaaagtt agcgcaagtg cgctgcgcgc gaattcggga catttaggta taggcttagc
     1381 aattcagtac tcaaacaaac ttgaaaataa aattattcct attgttgatc cggcagagag
     1441 cgcaaatgaa acagatacag aagttgttac gtcaagcgca attagaatta ttatgcatat
     1501 accaagtgca tttaagggtg ctggccgtcg cttactaaag aatgagaata aagcaatgta
     1561 agccataaag gcagctcgaa gtgctgaaat tggaactgcc gcacaaatta aatatgacaa
     1621 tataaagatg ctttggataa aaattatcaa tcatttttta agtcgcaatt tttttaacaa
     1681 cactaacaag attgctgtca ctaatgataa gtgggcgccc gacaccgcta ctaagtgagc
     1741 aagtcctgcg catttaaatc cgtcatataa atctcccgaa tttaaatcta ttctttgccc
     1801 gcaaataatt gcgcttaaca attgttcagc ttgcgaagta gaatcatcac tgtttcaatc
     1861 aaaatttcga atagtggtta ttagatgatt gcggaatgta aatattagtc caattggatc
     1921 agtccgtgta agtttgctag cttcgctagc tcgagcattt gcacatagtc cattttgcca
     1981 ataattgctt gtttgcaatt caatgggtga tgaaaatttt acctttgcct ttataatgtc
     2041 cccggcatga acttcttcaa aatcttgagg cacttgcaat ttaactttat agtttgaacc
     2101 agaaattttt gcatcgcaat tatttgtata ggttccaact ttttggtctt cttcgacttc
     2161 gaagacgtat tcgccttcgc cagcgcttaa gatattatgc atgttattgt ttagattatt
     2221 tgcaaatatc acagacaggc aagctccaaa aagtatcgcg atcaatcaaa taataatttt
     2281 gttaaaacct ctaagtcaaa gcagaagtcc caatatgaat atagctataa gagaagcaat
     2341 tattatccat atgtaaaaat tttgaaatca atcttttagg acataatttg ccagagttga
     2401 atatagcaat gccgaagtgc ctcataatat gaggccaaat tgcaaaatta ttggaatgct
     2461 tgggcgtttt ggaacagtgt ctagacgcaa atttggtcct taaatttagc gaatgtcttt
     2521 tctccaatcc cgcttatctt ttttaaatca tctattgatt taaatttccc gtttgcattt
     2581 ctatagtcaa taatcttttg agctgttgaa ggtccaactc cgtttagcgt ttgcaatgta
     2641 gttaaatctg cagtattaat atttaccttt ccattattag ttggcgaaga cgaagaacct
     2701 gccgcacttt cagctccgcc ttttgccggt atcataattt gctcaccatc ttcaacaagc
     2761 tttgcaagat ttaatgaatc tttatctgcg ttatctgtaa aacctccagc agcgtctact
     2821 gcatcattta ttcgagctcc ttcttctaat ttatagaggc caggtttttt aacttcacca
     2881 gccacgtgaa cttttatttc tgattgtttt tgttcaaagg agtcttcgct ggcctcaacg
     2941 gatattgaat tggagcccga aaagaaatta aaaagagcaa ttactataat tagagctact
     3001 aaaagcacta ttccaagagt ggcaattctt tttgtgtcaa acttttttga cgcaacttct
     3061 tcttcattat cttttgcatt attaaaagtt caaggcaaat cagaatcgct aatattttca
     3121 ctcttaagtt gtccttttag gtcatctaaa aaactcataa ttgctccttt cgtaagtggg
     3181 atcattatat tcataaacaa ttaacccgcg cagaaggcgc aatgaaataa ctatgaaaaa
     3241 tacttaactc agaataaaaa ttttgaaaat ttatgaaaat atttttgaaa gataatgaaa
     3301 attaattatt gaacgtgata gaataataag cagaaataat gcgaaagaag gtctaaataa
     3361 tgcctggtga aaataacatt tctgaaaata gaataaaaat tgatgaaatt gataaaaaga
     3421 tcattgaact gcttaatcaa agagcggatc tttctttaac tattagagat attaagaaag
     3481 caagtggtgt ggaaatctat gatccggctc gtgaagaaga aataatagat gctttatgca
     3541 aaattaatgg cggtccatta gacgataaaa gcattcgaga attatttagc aaaattttag
     3601 aagttatgaa aaagcttccc gataagaaat aattatggca cttgattttt cagaattaaa
     3661 cccagctcaa aagagcgcag tggaaacctt ggaaggcccg ctgcttgtac ttgctggtgc
     3721 tggttctgga aaaacaaaag ttttaactta taggatcgca aacattttgg acaaaggtct
     3781 ttgtagtcca tgaaatattc ttgcaattac ttttacaaat aaagcagctc aagaaatgcg
     3841 aagcagaatt aatggccttg ttggtgatgc ttcgcggggc atgtggattt taacttttca
     3901 tagtatgtgc gttcgaattt tgagagcgaa cgcggaggca cttggcttcg gcaaaaactt
     3961 tactatttat gatgatgttg atacgaagcg cctcatccgg cttatttgca tcgaccttaa
     4021 tataaacacg aaaattttcc ctgaaaatat gattcgtgga aaaattagtg gtgcgaaaaa
     4081 tgagctcatt ggtccaaagg aatatgataa gttggcgcat gatcttccga gcgaaaaagc
     4141 tgcggaggtt tattttgaat atcaaaaacg attgcacgat gctaacgcct tcgacttcga
     4201 tgacctgctt atgtatacat attatttgtt taagcgaaat cctgaaattt tgcaaatgta
     4261 tcaaaatcgc ttccaatata tttcggtcga tgagtatcaa gacacaaaca aagctcaata
     4321 cgaaatttgt aagcagcttg cgggtaagcg agcgaacatt atggttgtag gtgatgacga
     4381 tcaatcaatt tattcgtggc gaggcgctga catccgcaac attctaaatt tcgagaagga
     4441 ttacaaacac acaaccacag ttaagctcga gcaaaactac cgaagcacca gcaacatctt
     4501 ggatgccgca aataacgtga ttgcaaacaa tgacaagcgc aagaaaaaga agctctacac
     4561 cgaaggtgaa gccggcgaga gaattggcgt ttatgttgca agcaatgaac gtgacgaagg
     4621 acgttggatt gcgggtgaaa tcgagcgtcg gcgtcttcgt aaagttcgtc ttgcagatat
     4681 ggctgtgttt tatagaacga atgcacaaag ccgcgtcctt gaagatatgc ttattcgcgc
     4741 aggtatacca tataaaattg taggtggcgt aaagtttttc gagcgcgcgg aaatcaaaga
     4801 tgttatggct tatctatcgc ttttaattaa tccacacgat gatgtttcgg cgattcgcgt
     4861 catcaacact cccaagcgag gccttggtaa aaccaccgtt gatcatatca agcaaattgc
     4921 aactttggac aagtgcagtt ttatggatgc gtgcgttcac gttttaaatg acgaaagtct
     4981 aaagcctcaa gctcaaaaat ctcttggtga ttttgttagt ctcatcaaga aaaattccga
     5041 agccgaggct caaggtgaca cgaccggcag tcttcgtctt cgtattgaga aatatattaa
     5101 aaatactggt cttatagatg ctttgaaggc cgagcaaacc actgaagctg acactagaat
     5161 tgaaaacatt aatgagtttt tcggcgtttg cgaagagttt tcgaacactc atgacgaaga
     5221 agaaatgatg tttgatgcgc ccggccccga agacgcaaaa attattgaaa agaatagcga
     5281 accagagcgg acaattcgcg gagatagtct tccagatttt ttggagtggg ttcgtcttcg
     5341 tactgatctt gataatttgg gtgcggtggg ggacgctgat aacaattcga aaaattctga
     5401 tgacgcaata actttgatga cggtgcacag cgcaaagggc ctggaattcg attatgtttt
     5461 tgtaactggt atggaagagg gaatattccc gcatgcgacc gccttcggtg atttcgaaga
     5521 aaccgaagag gagcgccgac ttgcttatgt tgccatcacg cgtgcacgaa aagagctgct
     5581 tttaacttgt gcgcaaactc gtcaactaca aggcatgaca aatgcaaatc caatttcgcg
     5641 attcattggc gagattccaa atgagcttaa aaaatcttat ggcgttggta gtcttgaata
     5701 ttcaggattt ggttgagaca agcgaggcga ccgacatggt attgctggca gtggcatttc
     5761 aaagaagaaa gcaactggcg atttcatcga tttcttcggt ggtaaaccag agcctattaa
     5821 tgttaagggt aggcttgtca aaaacgatac tgatttcaaa aaaggtgaca aaattcaaca
     5881 caaagtattc ggtgaaggca aggttacccg cgttgacggc gaccgaatct atgtaaagtt
     5941 cctccgcagt gggcaagaaa aaactctcat gaaagacttc gccccaattg taaaaatata
     6001 gaagataaat tttacactta atcttgaaaa aaaaaaaaat aaggctatac tagatagttg
     6061 gtttttgaaa ggagaatata tgaataaaaa atcagcaaaa tctaaattgt ctttaagttt
     6121 cattggaatt ttattagcag cccttagtct ttgttttttg gcttttactt ctgcaaatgc
     6181 agtatccgat gagccagaac ctgaaattcc tgatgtccca caaggcaata taatgcttga
     6241 agtgcaacgt tcagatttag gcactattca atatcaaatt aatggaacat tatatccatt
     6301 gacagaatct aaagtattga cagcagaaga tatgaaggac ctgaaagaga tttacttgtt
     6361 gataaagcca aatcaagatg ctattattga taaagaatca acgattattc ataatggcga
     6421 taattttgtt tcacttaagg atcaagatta catagattta gaagcgggaa aatataaatt
     6481 aacttataat ccaaatgcta aaaaaggtca atatgcaaaa ttagctattt tagaaacacg
     6541 tttagttaca gtttcgctta cggatgccag tagcaaaatg cttgctaaca actttacttt
     6601 aatgctgggt catggtgata aaaatgatgt caaagttaca gacataggaa caatgaataa
     6661 tattcgtatt gataaaggta ctacagataa gttgattatg tcttttgcta ttagaagtga
     6721 gacatacttt attccgtata ttacagtaaa tggacaaaag tatgaaaccg ttgatactgg
     6781 catgcatgat gaagaggaac atgtaattat ttgttcaaca ctagaaggcg atgctgctaa
     6841 acccgatact tttcaggttg atttaagcat ttacgctcgg ccagaatgtg aatctcgttc
     6901 cgaatctatg aaaattgttc aagaggcaac atcgtatgat tatatgattg atggagtagt
     6961 tgtaaataaa ggtgattata aattaagtgg ttctgataca ccaactgacc cacaggtaga
     7021 aaatgccgtt gctacttatg atttagagct tactaaagat agtattcccg aaactgatat
     7081 aactggtggc atggatatac atctattatt aaaaccatca acttacagtg tacaagatta
     7141 caaagttgtt cgcaatcatg atggagttgt tgaagagctt gataccacat gcgaagttgt
     7201 tagaaaccgt gaaactggtg aagttgaagg agttgatgtt ggatttaatt ctgataagtt
     7261 ttctgacttt tcggttgttc ccgtgaactc tcagcctgta gttcctgaag acactcaagt
     7321 tacagctcaa actggtgata atattcctta tggtattgca ctagttgttc ttgctgtttc
     7381 agcatttgct gtttatagaa ctgctcgtag acctaaaggt gctcacgtaa aataagcgtt
     7441 cactaaatag aatttaaaaa cctcctcagc aatgaggagg ttttttattg gtgaagattt
     7501 gagttgtcgg ctcatacaac cctagctcca gatgcgcagg gagcagtatt tgcgttcttc
     7561 tccggtgacg ggttgatcga aaatgttagc ataggtgacg attcggtcgc attggccaga
     7621 agaggcgagg tagtcgccat aatcatcaga tgcgtttgga cgatcaagaa cgcagtaagt
     7681 ttgatttgta aggtccactg cgcaaactgc caaggttgat cgaatgatga agtagttttt
     7741 aaaccaggcg ggagcagcag aaggatttcg tgaaaagttt aatcacttta ggttggtgta
     7801 atcatcgtta gttttatcca ttggcgtgta agtgccgagg tttgatatgc cttcgccata
     7861 attataaatt gcatcgaagg aaaagttgaa cccagtttga ccataaccag cttcaagtgg
     7921 tcgcatattt tgaggaaggc agatgctgtc ttgaacttcg cctttattat ttatgtaggt
     7981 gagctggtag taaatgtttc cagactttgc acgcggagta ataacaacac cgtctttgta
     8041 agaataaacg ggcgtagaca ttctgcccat tgaagtgtag acggtgtttt tattgtcggc
     8101 aacatcattg cttacttcaa ctttcgaagt tgtgttctcg tttgcgctaa gactaaacaa
     8161 gtcagccttt ttaagtgagg agaggaaagc tttctttggg ccatttgcat ttggaagaac
     8221 ttgccaatat gcacaattac ctgctacagc aattgttggt gtctcccaat cagaacctcc
     8281 ggtatcaagc agtacaggat ttgcagtgta acccttgcta aagtccgaag cgtaaattcg
     8341 tcattcgcct tgcaaaatgt tgacttcggt tcaaattatt ccgctatcat tggcacgcgc
     8401 gtcgtaaatt tcataacctt cacgctgacc aatacttgat gggagtatgg ttgtttgact
     8461 gccgctatcc atattaataa tatcgacagt tgcaagtggc tttgccttat catttgggat
     8521 tagacaagtt ataaaactac tactgtttgc tcaagcaagc gatccatatg gaagttcaat
     8581 ttcgcgcgcg acacttaaaa attttgcttc gctttctgcc aagttgtccg aattaacaac
     8641 atcttttttt ggaacgctaa ttgttttaat agaatttaca acattactta ttgtgtcagt
     8701 tgcaacttta agtccagttc caacagcaag caatcctcct gctccaatag ctccataaat
     8761 taaatttcga cgagtgagca aaagcttacc gttttcatcg cggttgaaaa acggcttttt
     8821 cttttcttta aaatcagaaa catttccgat tggtttgatc tttttattct tttcgttaat
     8881 cgtgggcatc tgcttttctg cagatttttc gcttggcttc ttatcgacct tagggcttgg
     8941 tactttttga gctgcagcca cacttggacg acccttctca ttctcttcaa gacgcgcttg
     9001 attaaggtca acaacacctt gtgtgttctt ttttttacgt gcacacatgc gaattatttt
     9061 agcatatcgg cactattttt acgcttttat gataaactat gctggaatta aggagtaaaa
     9121 atgagtcaac gaaacccaat gaatgagcgt tataccaccg aagctaaagc cggtggtcat
     9181 actcgaaagt cagcggcttc tgctaaaccg aagtcgagcg ctgctgcttc ggtagttatg
     9241 ggttcgtcta agaaaaagcc tgctaagaat gctaaaaatc aaccaaaatc taagcgtgag
     9301 cgtaagcgtg aagaaagaaa taagcaatac gaagctgaac gtcgttatgg cgatcctcca
     9361 acaagggctt ttaaaattgc gaagcgtatg tggattggat ttttaattgc atctgttgtg
     9421 acagttgctc tttcatttgt tgcaagtaga attgaaggtt tgccagaatg aatgccaatg
     9481 gcctttttga ttgctgctta tgcttgcatt atcgttacac tttacctgga tttgggaaag
     9541 attcgtcgca tgcgtaaaga atatgttgca aaaatgagcg cttcgcaaac taaggaaagc
     9601 cgtgctgagc aaaagaagag aaaagctgcc gagcgccaag ccgcaaaaga agcagaggct
     9661 aaacgcatcg aagaagaaaa gaagcgagcc gaagaaaaag ctaaaggcaa gtctactcgt
     9721 gcaaacatga gttttggcgg caaagttaaa tcatttttca aggtcaatgg cgattaaatg
     9781 aagtttgttt cgtgaaacgt taatggcctt cgagcagttc taaaaaaaga tttcgaaaaa
     9841 acatttaaag atttcgatgc tgatattttt gctattcaag aaacgaagtg tcaagaaggc
     9901 caggctgatt tagaaacgcc gaagcatctt caatatttta actatgctgt tcgcaagggt
     9961 tattcaggta ctgcagtatt tacgaagaag gagccgttgc aggttcttcg tcacatgtgg
    10021 aatgaaaaag attgaaagcg cgatgaggat atagagacta ttgataacga aggccgattg
    10081 cttgcgcttg agtttgataa gttttggttc gtttgtgttt atactcctaa tgctcaaaat
    10141 gagcttgcgc gaattacacc tcgattaaaa tggggagagc gttttactga attttgcaaa
    10201 gaacttgagt ctggagccca cggcaaaggc aagaagcctg ttgttatgtg tggtgatttt
    10261 aatgttgctc acaatgagat tgatctaaaa aatcctgccg ctaatagggg aaacgctggt
    10321 ttttctgacg aagaacgcga agacttcacc aagctattgg acgctggctt cgttgataca
    10381 tttagaaatt tgtatccaga caaaactgac gcttatagtt ggtgaagtta ccgcgcgaat
    10441 tctcgacctc gaaacgttgg atggcgcatc gattacttct taattagtca aagcgcaaaa
    10501 gataaagtca aagattccat tattaacgct gatgttatgg ggtcggacca ctgtccaatt
    10561 acgttgaaca ttaactttta attttgtaga taaaataact accaacagaa attacagcgc
    10621 atcgaattag gtttacgctt atcagcgaaa acacagcact aactatatta agtataaaaa
    10681 tgttttctgg atatttgcgt ttgccaagag cgcacaagct aattatagaa atggccaagg
    10741 aaaaagcaga tgccagcaaa gaaaatattg tccatcccaa aattaaaccg aggccaaagt
    10801 tgaagagatc gaaaatgtta ttgacagtat caccgataat taaattttca ttttctattg
    10861 acgtgttgta accaagcggg gtatttcctg caatatttag agtaaggtct tcaacatgca
    10921 caaggccaaa agcaagtact ataaaaaaga caccaagtaa agcaacaaat attgaaaaag
    10981 cagacaagct gatcgtagca atgctcaatc ccttaactgc agttcgttgt ttttttattt
    11041 cagttttctt cgtcatattt tcctcaaaca ttcgcaatag tctatcacaa tgtctagatt
    11101 cttaaatatt taattatgtg ataaaattgt tttatggaaa agttaggcaa aattattttt
    11161 aacgaatacg aaattcaggc tcgcgtttct cgcgttgctg atgaaattaa tcgtgatttg
    11221 aaagataagg aaggcgacgg aattgttgtt gttggagttt taaacggggc ctatgttttt
    11281 atggcagatg ttgttcgtca tttgaatttt gaacttgagg tcgattttat tcaggcttcg
    11341 tcatataaaa attcaatgca gagcagcggt tatattaaga ttgagaaaga cctaacctcc
    11401 attttgcaag acaagcatgt acttattgtt gaggatattg ttgatactgg acgcactcta
    11461 acttttttaa aaaagctttt cttagataga aaggcgaaga gcgttagtat ttgtgccttg
    11521 cttgttaaga atcattcgga agccaaaaat ttggttgatt ataaatgctt cgattgccca
    11581 gatgaattta ttgttggtta tggattggac tacagacaga agtatcgcaa cttgccttat
    11641 attacttcga taaatacgaa gtaaaagcgc aaaaatctca cttttgatat ataatagcaa
    11701 acctgactgt gaaggttata ttttatggat gaaaaagaga aacatacaca ggacaaacgc
    11761 gaagctaaag agcaaaagcg ccaagataag attaagaagc aaaaagataa agaggcgcag
    11821 ctccaacgtg atattgcctt gatgaaaaag gaaatcgagg aaaataagct taagcgtggt
    11881 tccggcagtg gcggcgatgg ccagggtaaa aagcctaaaa aaagcaccat cgttattatt
    11941 gctattgcag ttggtctgtt gctgtttttc atcggaaacc aagtctttaa ttccgctgat
    12001 tcgcttatga cagacgagct taccacttcg gaatttattc aagcggttga acaagacagg
    12061 gttcaaaatg taatttacaa caccggcgat tatgttgttc atggttcata tactccaagc
    12121 aaatctatag gtcaaaatag tgctgagact ttcaacgatg cttttaaggc tcttaacgaa
    12181 ggcactctta atatcttaaa ccctgttcaa acacaaaata tcgaaggtca gggagcgcct
    12241 acaagaaatt ataaggctac atttgttggt caagattcgc ttcttgagct catgcaaaag
    12301 catcctaata ttcaatatga aattgtgttg ccaagtggct tcgctgatat ttttatgacg
    12361 ctacttccaa ttatcgcaat tggtattttg cttttcattt tcttctctca aatgacgaag
    12421 atgaacaatc aacaaatggg cttcggcaag accaagtcta agaaaagtga gcaggagcga
    12481 ccggacgtta agtttgccga cgttgctggt gtggatgagg ctgtcgaaga gatgagcgag
    12541 gtcaaggact tcctcagtaa ccccaagaaa taccaggata tgggtgctaa aattccgaag
    12601 ggatgcttgc ttgttgggcc tcccggtact ggtaaaacat tgcttgcgcg tgctgttgcg
    12661 ggcgaagctg gcgttccgtt ctttaacatt tctggctcgg attttgttga gatgtttgtt
    12721 ggtgttggtg cgagccgtgt tcgtgatttg tttaaacaag cgaaggaatg cgcgccttcg
    12781 atcatcttca tcgacgagat tgatgccgtt ggtcgccacc gtggtgctgg tcttggcggc
    12841 ggtcatgatg agcgcgagca aaccctaaac cagatacttg ttgaaatgga tggttttgat
    12901 aaaaacgatt cggttgtttt aattgccgca accaaccgtg ctgatatttt ggatccggcg
    12961 cttcttcgtc ctggacgctt tgacaggcaa attgttgttg atgttccaga tgttaagggt
    13021 cgtgagaaaa ttttaaaagt tcactcaaag ggcaagccgc tagataagga tgttgatttg
    13081 tggcgagttg ctcaattgac gcctggtttt accggcgcgg accttgctaa tttgatgaac
    13141 gagtctgcac ttcttgctgc acgtaaaaac aagaagacta tttctcaact tgaagtatca
    13201 gaaagtatgg agcgcgttat tgctggtcct gaacgtaaaa gcagaatcat cgatgacgaa
    13261 accaaaaaga caatcgcata tcacgaaagt ggtcatgcgc ttattgggca cttgcttcca
    13321 aaagcagatc ctgttcacaa gatttcaatt atttctcgcg gtcgcgcttt gggctacacc
    13381 ctctcaattc caaaagagga caaggtccta aattcttatg gcgagatgat ggatgagctt
    13441 tgtgttttca tgggtggtcg tgttgccgaa gaaattttct gcgaagatgt aacgaccggt
    13501 gcaagcaatg atcttgaacg cgctacaaag atggctcgtc agattgttac acaatatggt
    13561 atgagcagcg agcttggcac gcaggtcttc ggagagccta accatgaagt tttcttgggc
    13621 aaggattatg gcaacactaa agattattct gaaaagaccg ccgagcgcat tgacgaagaa
    13681 gtttcaaaaa ttatgaaaaa tgctcacgat agggcagtta tgattctgtc caacaacaaa
    13741 gagcaaatgg atacaatggc cgaagttcta attgagcgcg agaccatcga aggggaggcc
    13801 tgccaggcgc ttcttgataa caagtggcag gaatatgtgc tcaaagaaaa ggccgggaat
    13861 ctaaagtagg ctgccatgtc tgctcaaaaa caagaccaaa ttgttgctaa taatcgcaag
    13921 gctttccacg attatgaaat tcttgataag tttgaggctg gtattgagct tacaggtgcc
    13981 gaggttaaat ctcttcgtga aggacaatgc caactgaaag attcattcgt atttattcgc
    14041 cggggcgagg catggcttta caacgtgcat atttcaccat ttagaaatgg caacattgcc
    14101 aatcgggatc ctgagcgcaa gcgcagattg ctacttcaca aaaaagaaat tcgtaactta
    14161 aagcaagcaa ctcaggaaaa aggaatgtca ataattccgc ttaaagtgta tttcaaagca
    14221 gggcatttgg taaaggttga aattgcggtc gcccgaggca aaaagcttca tgacaaacgt
    14281 caggcaattg cgaagaagac tcaaatgcgt gaagctgaaa gttatttaaa gtcaaggagt
    14341 aggtaatggc aaaggctgaa gaaatcaacg aatctcaact tagtgatgat gttttcgaac
    14401 ttgacttcga cgaggaaagc gttgaatatt atatagttga tgaagatgac aatgaaattg
    14461 gcgtttgcct taatgaagat ggccaacttg ttgaatatct ctacgaagat gagattgaag
    14521 ctgagcctca aattcaagac cattctgcta atagggctgt aaaaaagcag gttgatgatg
    14581 ctgctcagag catcaaaaat atgcgtgatg agcttgggaa tgcttccgaa gacgctaaag
    14641 ctgttgctca agagctaaaa gacactgccg aagagcttca aggcatgtta aacgaagtaa
    14701 aagactcctt caaaattttc ccatccaaaa agaaaaaata ggttaaaact taagttgtag
    14761 tggtaatata cgtggtccgc gtcaataggg atgcggaaaa ataagctagt ctgtgtgacc
    14821 ggcgtttgct agtgaccaga gaaaaggttg caaacaatgg taagaccaca gaccgaagag
    14881 taacgaatag gagttttaca atggaaaaag ttagtattgc ttcgcttctc gaagcaggtg
    14941 gacattacgg acaccaaacc aaaaagtgga atccaaaaat ggaatcctac atcttacgca
    15001 aacgcggaga cattcacatc atcgatttaa aaaagactct ttatgcaatg gacgctgcat
    15061 tttcatttgt gcaagaaact gttgctaagg gcggaaatgt cttgtttgtt ggtaccaaaa
    15121 agcaagctca ggatgctgtt gttgagcagg caagccgttg tggtatgcct tatgtaaatg
    15181 ctcgctgact tggtggtatg cttacaaact tcgacacaat tcgcaagagc gtaagcgaga
    15241 tggaaaagct tgaggctcaa gaagaggacg gcacaattgc cagccttaca aagaaagagc
    15301 aaatcacact tcgcaaggaa atggctaagt tgcaaaccaa cttaaatggt attcgcaata
    15361 tgaagaaaaa gccagcttgc gtatttattg ttgatacaac tcgcgaagat attgctgttc
    15421 gcgaagcacg taagcttggc attccaattg ttggaactat tgacacaaac agcgatccag
    15481 ataacatcga ttttggtatt ccaattaacg atgatgcaat tcgctctgtt gagcttgttg
    15541 ttgagtttat tgcagaagct gttttggctg gaactggcgc tgctggtatg gaggcccaag
    15601 ctgctcaagc taagaaagat gaaaagaaag ctgaagctaa aaaattagta gatgctgaag
    15661 ctaaaacaat tgatgaggtt cttgctgaac ctgctaaaga agaaaaagca gaagatgcga
    15721 aggaggaaaa ataatggctg agattactgc tgctatggta aaagacttgc gcgaggctac
    15781 tggcgcaggt atgatggagt gcaaaaatgc acttaaagag gccgacggag attttgacaa
    15841 agctgttgat gttcttcgca caaaaggttt agctgctgtt gaaaaaaagg ctggacgttc
    15901 tacaaacgaa ggaacagtta tggctattgt ttctgacgat ggcaaaactg gagctgttct
    15961 tgaactaaat tgcgagactg acttcgttgc tatgaacgaa aaattccaag cttatgcaaa
    16021 gcgtatcaca actgctgctc ttgaaaacaa accagctgat gttgatgcat tgaaggcttg
    16081 cgaagaaggt ggggaaaccg ttgaggcaat tttgactgac gcaattcatg taatgggtga
    16141 gaatactcaa attgctcgct ttaaggttgt tgaagctcca gctatcattt cttatattca
    16201 cactggtgga aaaattggcg ttttgg
//
LOCUS       DBNCDC010000012        15921 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_1170_length_15921_cov_6.207803, whole genome shotgun sequence.
ACCESSION   DBNCDC010000012 DBNCDC010000000
VERSION     DBNCDC010000012.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 15921)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15921)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15921
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1170_length_15921_cov_6.207803"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="AC04VQ_01785"
     CDS             <1..909
                     /locus_tag="AC04VQ_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIO9552964.1"
                     /translation="VGVIAAQSIGEPGTQLTMRTFHSGGVAGEDITHGLPRVQELLEA
                     RNPRGVALLADIDGKISVKKAGDEQEITIQGKSKAKTHTVSGHAQLVPGLKNGDTVTV
                     GQQITRGSINPKDLLKLTDAETTLGYIVNEVQRVYVSQGVEINDKHIEVIARQMLNKV
                     IVDKSGDTDLIPGQQINRYKFRRIVEECIKDKKQAPEGHDKLLGLTKASLSTDSWVSA
                     ASFQETTKILTDAAIAGKVDTLQGLKENVCIGKHIPAGTGLKRYRDMSLLYKGQKIEG
                     AEGEELPESAPEELRNLESMIPKHES"
     gene            937..1479
                     /locus_tag="AC04VQ_01790"
     CDS             937..1479
                     /locus_tag="AC04VQ_01790"
                     /inference="COORDINATES: protein motif:HMM:NF015102.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HIO9552965.1"
                     /translation="MGFDDNSYYDSMKYLSRAKLTNEEARLYLYDDLKVSQRWANKFS
                     EASIETVADLIGHTEKDLLKIEGIGVKAMDELKAGLKKRNLLHVIEDDLEATDDDVSS
                     LLDMVFSPDGSVSEEGAAPAKDADESYDDTDLLDDEGVVKGDKKAKSDDLDDLLKDLG
                     DGVSITDEQLADDILKDDEE"
     gene            1700..2797
                     /gene="queA"
                     /locus_tag="AC04VQ_01795"
                     /pseudo
     CDS             1700..2797
                     /gene="queA"
                     /locus_tag="AC04VQ_01795"
                     /inference="COORDINATES: protein motif:HMM:NF014592.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
     gene            2998..3216
                     /locus_tag="AC04VQ_01800"
     CDS             2998..3216
                     /locus_tag="AC04VQ_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0031564 - transcription antitermination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination NusB family
                     protein"
                     /protein_id="HIO9552966.1"
                     /translation="MADGKQRRKSRKVSLQVLFANDVVGVNTSDVLTGKATLPHIGEI
                     DEYARGLIEGTVKNMSEIDGKLVSVSEN"
     gene            3229..3417
                     /locus_tag="AC04VQ_01805"
     CDS             3229..3417
                     /locus_tag="AC04VQ_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013216.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIO9552967.1"
                     /translation="MPATDRCLLRQSVYEMLYVEDVPISVSINEAVELAKEFGGDDES
                     HKFVNGVLGKIAKELEEE"
     gene            3430..3729
                     /locus_tag="AC04VQ_01810"
     CDS             3430..3729
                     /locus_tag="AC04VQ_01810"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF014648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIO9552968.1"
                     /translation="MSEEIKNENVEEVEEVEVAEEAASEPPRELKDYEKIESRLKEIE
                     QTITTENLDMDESLKLYEEAVDLGMKASQTIENNVLVDMNNETEEESQDNQETEE"
     gene            3733..5157
                     /gene="dxs"
                     /locus_tag="AC04VQ_01815"
                     /pseudo
     CDS             3733..5157
                     /gene="dxs"
                     /locus_tag="AC04VQ_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024688.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
     gene            5200..5643
                     /locus_tag="AC04VQ_01820"
     CDS             5200..5643
                     /locus_tag="AC04VQ_01820"
                     /inference="COORDINATES: protein motif:HMM:NF014799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIO9552969.1"
                     /translation="MQGQTKKEIAKFKIGKSRTLKSGNDIAILAFGDSVLDALEAHQM
                     LKDRGIKCRVVDMRFVKPLDSEAIADAALCTHRILTVENGILSGGAGESVLAELSKMN
                     LCDGLKFRALGINDRFVAHGEVDKLKTAANIDYKSIFDAAVSLLK"
     gene            complement(5644..6207)
                     /locus_tag="AC04VQ_01825"
     CDS             complement(5644..6207)
                     /locus_tag="AC04VQ_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552970.1"
                     /translation="MMYYLRETLVNLAKDGIKYAAKFAKDQYPKILSHKDKFEKKVSD
                     AAEDAVPAVEKAQNTFSKFVMDKYSEAVNLTKKAEEEKKKNRMKAIRNGIIATTIGFG
                     AGAATGYLLVKKSQIKESDYVFSDEEISEDVTDPKMTAIPGGKAVEPDPQPESPFEKI
                     AQQEQKTYQAFNDTYQISTDGTTLIKR"
     gene            complement(6226..6301)
                     /locus_tag="AC04VQ_01830"
     tRNA            complement(6226..6301)
                     /locus_tag="AC04VQ_01830"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6266..6268),aa:Ala,seq:ggc)
     gene            complement(6381..6632)
                     /locus_tag="AC04VQ_01835"
     CDS             complement(6381..6632)
                     /locus_tag="AC04VQ_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIO9552971.1"
                     /translation="MICAFAPPSNEDQRNLGRSQRILNISNKFVVTKDLIYALNSGYS
                     EAVLIDDVFTTGATLFSASQVLKEAGASHVFCANFSRTF"
     gene            complement(6833..7084)
                     /locus_tag="AC04VQ_01840"
     CDS             complement(6833..7084)
                     /locus_tag="AC04VQ_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552972.1"
                     /translation="MKKYQLSKTQLPKLYDQPAAQFAIGQVTCFVQVAQENFPTSINQ
                     KLVKFAASPLAAFNVVDAMAALRLINATVLSFLTKTLAS"
     gene            7378..8232
                     /gene="mltG"
                     /locus_tag="AC04VQ_01845"
                     /pseudo
     CDS             7378..8232
                     /gene="mltG"
                     /locus_tag="AC04VQ_01845"
                     /inference="COORDINATES: protein motif:HMM:NF014656.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
     gene            8233..8766
                     /locus_tag="AC04VQ_01850"
     CDS             8233..8766
                     /locus_tag="AC04VQ_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HIO9552973.1"
                     /translation="MSFTLPDRYLKRISDIDIQKDLINAGYKFVLLDVDNTILTRDES
                     VVPDDVQKWLAKARKAGLKFCLVSNDWHKNVVELANEIKLPIVVKSLKPLPAAFYRAR
                     KKIGANKQNCIMIGDQLMTDVFGAHVVGLKCILVQPLVEKDLIHTLMLRKIEKVFLRN
                     IEPENRKAGSKVEKTKN"
     gene            8984..10114
                     /gene="aroC"
                     /locus_tag="AC04VQ_01855"
     CDS             8984..10114
                     /gene="aroC"
                     /locus_tag="AC04VQ_01855"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIO9552974.1"
                     /translation="MQYLTAGESHGEKLVIIIDDVPCGVSLSEKEINFDLKRRSSGLG
                     RSVRQDSESNACKIVSGVKDGKTTGNPVCIEIPNSNDNPESNNNIVRPGHADLNGVIK
                     YNLDDCENVAERASARETAARIVAGVIAKNMLAEFEVEVYGFVTSIGAVKIKLDETNM
                     PGTLPDMSSIAMSEVMCPDAKASEKIIKQIDRAREAGESLGGSIRLVAVGLVPGLGGY
                     SQGYSRLDGKIAAAVASVPSVKEIEFGSSAYVSENVGSVGLDNIVKDANGGFVRETNF
                     AGGLEGGMTNGMPLVVSAKVRPCPTIKKPVKTIDLETMDGVVVASDKRSDVCVVPNVA
                     VVCESELALCLANEFMDKFGRDSLSDIKFAVDAYKHRIKEMK"
     gene            10121..10660
                     /locus_tag="AC04VQ_01860"
     CDS             10121..10660
                     /locus_tag="AC04VQ_01860"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIO9552975.1"
                     /translation="MWGSQRYLIRKPVALIGFMGAGKTSVAKLLEEHTGIKAVDTDNA
                     VVQATRQTVSEIINNQGEEEFRRVEAKVLGELVEEQKLIIATGGGIIESAVSRGILKQ
                     CYCVWLKVDAKNSAKRIEDFSTRPMFKDIENAENLLARRNEIYEQYSDVAVNTNNKPL
                     SAVVREVRDNLLKAGILQY"
     gene            10671..12317
                     /locus_tag="AC04VQ_01865"
     CDS             10671..12317
                     /locus_tag="AC04VQ_01865"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033840 - alpha-1,4-glucan
                     glucosyltransferase (NDP-glucose donor) activity [Evidence
                     IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HIO9552976.1"
                     /translation="MASKSSNKKVKVLYSAFEAVPFIKLGGLGDVAGTLPASINVHNC
                     DCRVIIPYLSMISKQHQMLFKHIGDYSVFSAGEEREFGLYFVKLRGVTYYLIKNDHYF
                     NRKSAYGEKDDIERFAFFSRAILESIKHMKGFKPQVLHCNDWHTALTNLFLHEEFEDD
                     LGALKIKTVFTIHNLQYQGKFKYEQVKDVLNLKNRSAVAKVMHKGKVNLMKCGIVSSD
                     CVTTPSPSYAKEIMTGEYGVGLENVLRTKKDVTQGILNGIDTVDFNPAWDTNIRVHFT
                     KELMNRRLGNKIDLQKKFKLDVDVDAPLFCVACRFYEQKGIDLIIKGVPKIMNKGGQI
                     VFLGDGDDAYKKKIRDYKKKYKGRVGMHLKFDPQLPHNVYAGADVLLMPSKFEPCGIA
                     QMMAMHYGTLPLVRSTGGLRDTVHDYKAKSLISNGFVFKNYNLKEFNAALDVVFDVWN
                     NDKKNWARMQRRGMEEDFSWKESGRKYSVLYRALLDGKITKVDEIFEAEQAKRAAQKA
                     KEQAALAKKNAKASIKMAQKVAEEASNYEFVDISQTTDED"
     gene            complement(12371..13156)
                     /gene="rpsT"
                     /locus_tag="AC04VQ_01870"
     CDS             complement(12371..13156)
                     /gene="rpsT"
                     /locus_tag="AC04VQ_01870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIO9552977.1"
                     /translation="MANIKSQKKRIKTNEKAHVRNVAIKTELKTATRKVKDAVDAKKA
                     ADAYKFALEVCTLLDRACAKGVIHKNQAANRKSGIMALANSIVTDKERKEYKPEPKRA
                     PEKKGGTKKAKAKEERKAAMKKESEEKAKRREATLKLEKAAAKRKPKEEPPAEEGAEA
                     PADAPAPAEGEAAPAPEAAPAEEAPKEEAPKEEPKAEEKPAEEKPAEEKPAEEKPADE
                     KPAEEPAKEEAKEEPKEEEKPAEEPKEEKKEEAPKEEEKKEDK"
     gene            13526..14440
                     /locus_tag="AC04VQ_01875"
     CDS             13526..14440
                     /locus_tag="AC04VQ_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding periplasmic protein"
                     /protein_id="HIO9552978.1"
                     /translation="MTKKRSISNKLIVSLIAAAMALLLCVAVAGCAPSNYDPQLKDPQ
                     LKTPDILENGKLKVGVDFGNPPFAGETSKAAGIDIDVASAIAQELGLEVEFVDVGTSA
                     DLALSNKKCDMVMGVSKAVNVEEMWKSDVYIKTACALFAKDEKAAVPVAGGADKFATE
                     MASQSAIACQSQFSAANIKLNGTLSESFELLEKGDVKYVAADAVIGTYSVNNSDNEAY
                     LVALLSKVSGYCIGADKTKTNLTNKVSAILGNLAKNGVLETIEKRWLGKTLDLDKVQM
                     TAGAANQADAGELEAGVYSQTHPNNNTP"
     gene            14442..14705
                     /locus_tag="AC04VQ_01880"
     CDS             14442..14705
                     /locus_tag="AC04VQ_01880"
                     /inference="COORDINATES: protein motif:HMM:NF042468.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase PUA domain-containing
                     protein"
                     /protein_id="HIO9552979.1"
                     /translation="MSLQHFFLENQDFGSEVEASAGGSVTLSLSADDLHHAKVLRLKS
                     GEHIGIIDSKQKFFEVEIVEFSKDLLVKNSTKKTNTSLNNCHL"
     gene            14718..15221
                     /locus_tag="AC04VQ_01885"
                     /pseudo
     CDS             14718..15221
                     /locus_tag="AC04VQ_01885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
     gene            15329..15664
                     /locus_tag="AC04VQ_01890"
     CDS             15329..15664
                     /locus_tag="AC04VQ_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013112.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552980.1"
                     /translation="MQDADLVIVNTCTVTSVADKKTRQIVRKVCRDSKAQKIIVTGCA
                     SAIEPEVYEQIDSRIEIVAKADMQNAEADALRVLAGDEFRSRAALKIQDGCNNACTYC
                     IVHTARGSA"
     gene            15668..>15921
                     /locus_tag="AC04VQ_01895"
     CDS             15668..>15921
                     /locus_tag="AC04VQ_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylthioadenine synthetase"
                     /protein_id="HIO9552981.1"
                     /translation="MPHKQVEEQVKCMLDAGIKEIVLSGVDIGAYDDNGFGLSDLCKS
                     LLEIIGDRLARIRISSIEPNNVSDELIEIIAGADGKICRH"
BASE COUNT         4576 a         2910 c         3730 g         4705 t
ORIGIN      
        1 gttggtgtta ttgctgctca atcaattggt gagcctggta cacagcttac tatgagaaca
       61 ttccatagtg gtggtgttgc tggtgaggat attactcacg gtttgcctcg tgttcaagag
      121 ttgctcgaag cacgtaatcc tcgaggcgtt gcgctacttg ctgacattga tggtaagatt
      181 tctgttaaga aagcaggcga tgagcaagag attacaattc aaggcaagag taaggcgaag
      241 acacatactg tttctggtca tgctcaattg gttcctggac ttaaaaatgg cgacactgta
      301 acagttggtc agcaaattac tcgtggttct attaacccta aagatttgct caaacttaca
      361 gatgccgaaa caactttggg ctatatcgtt aacgaagttc aacgcgttta cgtttctcaa
      421 ggtgtagaaa ttaacgataa gcacatcgaa gttattgctc gtcagatgtt aaataaggtt
      481 atcgttgata agagcgggga cactgaccta attcctggac agcaaattaa cagatacaaa
      541 tttagaagaa tcgttgagga atgcattaaa gataagaagc aagctcctga aggacatgac
      601 aaacttcttg gtcttacaaa agcttcgctt tcaactgatt cctgggtatc tgcggcttcg
      661 ttccaagaaa caacgaagat cttgaccgac gctgcaattg ctggcaaggt tgatacactt
      721 caaggtctaa aagaaaacgt atgtattggt aagcacattc ctgctggaac tgggttgaag
      781 cgttacagag atatgagtct tttgtacaag ggtcaaaaga ttgaaggcgc cgaaggcgaa
      841 gaacttccag agagtgctcc tgaagagctt cgtaacttag aaagcatgat tcctaaacat
      901 gagtcgtgaa agattgactc tgaaggttat ttgcacatgg gctttgatga caactcatat
      961 tatgacagca tgaagtacct gagtcgtgca aagctcacta acgaagaggc tcgactctat
     1021 ctctatgatg accttaaggt ttcgcagcgt tgggctaaca agtttagtga agccagcatc
     1081 gaaactgttg ctgaccttat cggccacacc gaaaaagacc ttcttaagat tgagggtatt
     1141 ggtgttaagg ctatggatga acttaaggcc ggacttaaga agcgtaactt gcttcatgtc
     1201 attgaagatg atcttgaagc aactgacgac gatgtttcaa gcttgcttga tatggtattt
     1261 tcacctgacg gttcagtctc cgaagaaggt gctgcacctg ctaaggatgc tgacgaaagt
     1321 tatgatgata ctgacttgct tgatgacgaa ggcgttgtca agggagacaa gaaagcaaag
     1381 tcagatgact tggatgactt gcttaaagat ctcggtgacg gagtaagcat caccgacgag
     1441 caactcgccg atgacatcct caaggatgac gaagagtaat caatagggct ccgcaaggag
     1501 ccctttcttt taggtttttg ggagagattt cggatatacc tttctcaacg gcttcgcggg
     1561 cagcccatag tgcgttcgcc agcaagcaca cggcgctgca ggggtttcga aaggttatat
     1621 ccgaaatctc tcacttatga gccgacaact caaagcttca ccaataattt gtcaaaataa
     1681 tgtagtaaaa ttcacggcaa tgaaaactag tgattttgga tacatactcc ctaaagagct
     1741 tatcgcgcaa gagccctgcg aaaaacgcga tgcttgtcgt ctcttgtcat tagacaagag
     1801 cactggacag attgaagatc ttaaattttt cgaaatagaa aaattgctca aacccggcga
     1861 cctccttgtt gcgaatgaaa cccgagtctt gcctgcaaga ctccttggca agaagcgagg
     1921 aacctccgga gaagcagagc ttctgcttct caaaaactta ggagacaacc aatgggaagc
     1981 acttgttaaa cctggccgtc gcctaaagcc agcttcacta tctgaaggcc aaaatacaac
     2041 tcgatccgcc tcttccggag ataaaggtcc aatcgttgac ttttccgaca aagacggcga
     2101 agccgtcttg tcagcagaaa tcattgactg aggtagctcc gaaggagcta gaattgttaa
     2161 gctatcaagc gatatctacg atatcggtga agctatccac aaggtcgggg ctacgcccct
     2221 gccgccatac atccatggtt atactggaaa tatggaaatg taccagaccg tttactctgt
     2281 gaacgaaact tctgcggcag cgccaacggc tggtttgcat tttaccgaag aacttattgc
     2341 tcgcttacgc gagcgaggca tcggttgagc tacagtcgaa cttgaagtcg gcatcgacac
     2401 tttcaaacct gtcacagagg acgatccgaa agatcatcta atgcacacgg aacgctacag
     2461 cgttccggaa gagtgtgttt ctgctatcgc agaaaccaaa ttaagaggcg gcaaagtaat
     2521 tgctgttgga acaacaacag tccgctctct tgagagcgca tctcgtagcg gaacattaaa
     2581 aagcgtttgt cgtgatgcga cagatttgtt tattttacca ggttatgaat ttaagtgcgt
     2641 agaagcacta attacgaatt ttcatgttcc acaatcgacg ctaatgatgt tggtcaccgc
     2701 attcggcggt tatgataatg tgatgaacgc ttatgcgcat gcggttgaaa gcaagtatcg
     2761 cttcttgtcc ttcggcgacg ctatggttat taaatagttg gctctatcaa gattacgtct
     2821 cataagtgag agatttcgga tataaccttt cgaaacccct gcagcgccgg gtgcttgctg
     2881 gcgaacgcac tatgggccgc ccgcgaagcc gttgagaaag gtatatccga aatctctccc
     2941 aacaatcaaa ataaaaatta aaatgcgctg acgcgcattt ggtataatac tcgcgttatg
     3001 gctgatggaa aacagagacg aaaatcacga aaagtaagtt tgcaagtgtt gtttgctaat
     3061 gatgttgtag gtgtcaacac gtcagatgtc ttgacgggga aggctacact tccgcacatt
     3121 ggcgagattg acgaatatgc tcgcggactt atcgaaggca ctgttaagaa catgagcgag
     3181 attgatggca agcttgtaag cgtctctgag aattgagcgt tggacaggat gccagcgaca
     3241 gatagatgtt tgcttcgtca gagtgtttat gaaatgttgt atgttgagga tgtgccaatt
     3301 tctgttagca ttaacgaagc ggtcgagctt gctaaggagt ttggcggcga tgatgaatca
     3361 cataaatttg taaatggagt tttgggcaag attgccaaag aacttgaaga agaatagctg
     3421 aggtttacta tgagtgaaga aatcaaaaat gaaaacgttg aagaagtaga agaggttgaa
     3481 gttgccgaag aggcagcgtc tgagcctcct cgagagctta aagattatga gaaaattgag
     3541 agccgcttga aggaaatcga gcagactatc acgactgaaa atttggacat ggatgagtct
     3601 ttaaagcttt acgaagaagc agttgacctt ggcatgaagg cttctcagac tattgagaac
     3661 aatgtgttgg tcgacatgaa caacgagacc gaggaagagt cacaagacaa tcaagagaca
     3721 gaagaataat ctatgccgcg tctttctcga atacttgatc taattgaaaa cccgagtgat
     3781 cttcgcactt taaatgacga agagcttgag attttggcgg gtgagattcg tgaagaaata
     3841 attaatacaa catcaaaagt tggcggacat gtttcctctt cgttgggggc cgttgaaatt
     3901 attttggcgg ttcacagcat gataaatagc ccaaaggata agtttatttt tgacgttggg
     3961 catcaggctt atgcgcataa gttggttact ggaaggcgtg acgcgtttaa gacgcttcgt
     4021 caatttggcg gtttgtctgg gtttgctcgt ccaactgaaa gcatgcatga tgttcatttt
     4081 tcggggcatg cggctgatag tctttcggtt gctcttggtc ttgcgaaggc taaaaaactt
     4141 tctggcgggg atgagaaaat tgttacgctg attggggatg cttccattgc tggcggcatg
     4201 tcttttgagg ctataaataa tatcggtcat gaaaagttgc cgcttgttat tattttaaac
     4261 gacaacagga tgtcgatttc tgaaagtgtc ggaggagtcg gaaagcatct tcgttcgatt
     4321 cgtcttcatc cgcaatacaa tgatgctctt aacgctgtta agtatgggct aatgcgtttt
     4381 ggttcggttg gcgaatttgc tattaagtga ggccatgctt cgaaggaatc tctcaagcat
     4441 tttattatgc caaacgctat gatttttgaa gagcttggaa ttacttgttt gccaactgtt
     4501 gatggtcatg acattacggc gcttcgaaac acaattaagg atgctcttga atatgacggg
     4561 ccagttttgg ttcacgcaat tactcaaaaa ggtgctggtt ataagcctgc gacgaaggat
     4621 cctgaactct tccacggaat tggtgcttat aatattcaaa ctggataccc aattagtgag
     4681 aagccttatt cttttacgaa tgctttttcg gagcagttgt tggccatggc ttctagagat
     4741 aaaaagattg tagcaatgac cgctgcgatg tccggaggca ctgggcttaa gaagtttgca
     4801 gaaaaatatc cggatagatt tgtagatgtt ggaattgccg aagagcattt gcttggctct
     4861 gctgctggac ttgcgaaggc gggctacaag ccttttgttg ctatttattc tgcatttttg
     4921 caaagagcag ttgaccagat ggttactaat gttgccttag aaaaattaaa tgtaaaaatt
     4981 tgcattgaca gagctggact tgttggggca gatggtgcaa ctcatcatgg aatttttgat
     5041 atggcctata caaaaatgat tccaggcttt acggttcttg ctccttcgtg tgctcatgaa
     5101 ctaaagtgtg ccttgaaaac tgccgcatct gttgatggac cttttgcgat tcgttatcct
     5161 aagggaagtg caaaagatat cactgatacc ggaaaagttt tgcaaggaca gaccaaaaaa
     5221 gaaattgcta agtttaagat tggtaaatct cgaaccttga agagcggaaa cgatattgca
     5281 attttggctt ttggtgacag cgttttggat gctcttgaag cacatcaaat gcttaaagac
     5341 agaggaataa aatgtagagt tgtggacatg cgcttcgtta agcctctaga ctccgaagct
     5401 attgccgatg ccgctttgtg cactcacagg attcttactg ttgaaaatgg aattttaagt
     5461 ggaggagcag gagagtctgt tcttgctgaa ctttcgaaga tgaatctttg tgatgggctt
     5521 aaatttagag cgcttggaat taatgatagg tttgttgctc atggcgaagt agacaaatta
     5581 aaaaccgcag ccaatattga ctataagtcc atttttgatg ctgcggtttc tttattaaaa
     5641 taattatctt ttaattaatg ttgttccgtc tgtagagatt tgatatgtat cattaaatgc
     5701 ttggtaggtt ttttgttctt gttgagcaat tttttcaaat ggtgattcag gctgtggatc
     5761 tggctcaact gccttaccgc caggaatggc tgtcattttt ggatcagtta catcctcaga
     5821 aatttcttcg tcgctaaata cataatcgct ttctttaatt tgagatttct ttaccaatag
     5881 ataaccggtt gcagcgcctg cgccaaaacc aattgttgta gcaataatcc cattacgaat
     5941 tgctttcatt ctattctttt tcttttcttc ttctgctttt ttagttagat taacagcctc
     6001 agaatatttg tccattacaa attttgaaaa agtattttga gctttctcaa ctgcagggac
     6061 tgcatcttct gcggcatcgc taactttctt ttcaaactta tctttatgtg aaaggatttt
     6121 aggatattga tctttagcga acttagcagc atatttaatg ccgtcttttg ctaaatttac
     6181 aagcgtttct ctaagataat acatcattaa cctccaagtt atcaatggtg gagcataggg
     6241 ggatcgaacc cctgacctca tggctgccag ccatgcgctc tcccagctga gctaatgccc
     6301 caagcttttt atttcatttg aacttcgttt agcgtggact acgtattata taaaattatt
     6361 taaactacaa accataaatt ttagaaagtt cgcgaaaaat ttgcacaaaa tacgtgtgag
     6421 gcacctgctt cctttaaaac ttgcgaagct gaaaataaag ttgcccctgt tgtgaataca
     6481 tcgtcaatta aaacagcctc actatatcct gaattaagtg catatatcaa atcttttgtc
     6541 acaacaaact tattacttat atttaaaatt cgctgacttc tgccaagatt tctttggtct
     6601 tcgttcgaag gaggtgcaaa ggcgcatatc aattttacat ttaattgcga ggcaagttct
     6661 tcggcagtta attttgcatg gtcaaagcct cttctgagta ctgcgtcttt ggtggaggga
     6721 atataggtta aaaaagttga aggtgtaatc cagcaaggat taatcatttt gcttaagaaa
     6781 tatgcgataa ctttggaaag tcttcgctcc ccagaatctt tatatgttgt tatcagcttg
     6841 ccagtgtctt ggttaagaaa gaaagcactg ttgcatttat caaacgaagc gctgccattg
     6901 catccacaac attgaacgct gccaaagggc tcgccgcaaa tttgacaagt ttttgattga
     6961 tcgatgtagg gaagttttct tgagcaactt ggacaaagca agtaacctgg cctatcgcaa
     7021 attgcgcagc gggttggtca tagagtttcg gcaactgcgt ctttgacaat tgatattttt
     7081 tcatttttgc tcacgaccta tatcatactc agaaacgcta aactgccgca gaaggcccag
     7141 taaagattat taaacaaaac taaatttaaa taaaatatgt taaaatatat tgtgataaaa
     7201 atgcacaaag aaagaaaaaa accgttattt tttatcttaa taataagcgc agttatactg
     7261 gcaataatcg caattatctt ttttgctatt cccggatgtt caaaaactct tcctgcagga
     7321 caaactgctg aggtcacgat cgaagagggc tcttcgacct gaacaattgc tgacaaaatg
     7381 tctaaggctg gagttgttga aaaccccgct gtttttacgg caaccgttac agaaatggga
     7441 gccgaggctt ctcttcaggc tggaacatat atatttgagg gcggcaaaac tgttcgcgaa
     7501 tatgttgaga ttttatgcga aggacctgat aaatattcac caaaaattgt cgttcatgaa
     7561 ggtatgcgac ttactaaaat tgccgatgct gttcaagaag caactaagtc tcgaataaaa
     7621 gctcaagact tcataaatgc tgcaagtgat gcaagtgttt acgcttcgga ttatgaattt
     7681 ttaagcgatg ttggaaataa ttcgttagaa ggctttttat atccaaaaac atatgctgtt
     7741 agcagtacag attcggttgg cgacatagtt agaaaaatgc tcgatcaatt caaagaggaa
     7801 acaaaagact taaatttaga ttatccaaaa tctaaaaatt tgacattatg agaagcggta
     7861 actttggctt ctattgttga aaaagagtct gctcaaggtg ttcaaggcaa ggttgcaagt
     7921 gtattttata accgacttag cagcggaatg catatcaatt ctgatgctac aacagcctat
     7981 gaagtggggc atgatccaac gcctgaagaa ctccattcac aaagccctta cagcacttat
     8041 acaaattatg gattgcctcc gaccccaata tgttctccgg ggattgaagc tcttcgtgcg
     8101 gtttgtttcc cagaagagac aaattatctt tacttcgtat ttaagacgga tgaaaacggc
     8161 gagctccaat ataggtttag cgtgaccttc gaagagcatc agcaagccat cattgattta
     8221 gggttagttt agttgagttt tacactccca gacagatacc taaaaaggat aagcgacatt
     8281 gatatccaaa aggatttgat aaatgccgga tataaattcg tgcttttaga tgtggacaat
     8341 acaattttga cacgtgatga gtctgttgtt cctgatgatg ttcaaaaatg gctggcgaag
     8401 gcaaggaaag ctggccttaa gttttgtcta gtttctaatg attggcataa aaatgttgtt
     8461 gagcttgcaa acgagataaa attgccaatt gttgttaaat ctctaaagcc tctacctgct
     8521 gctttctatc gcgcacgaaa gaaaattggt gcaaataagc aaaattgcat tatgatagga
     8581 gaccagctta tgaccgatgt ttttggggct catgtggttg gacttaagtg tattttggtt
     8641 cagcctttgg ttgaaaaaga cctgattcac acattgatgc ttcgaaagat tgaaaaagta
     8701 tttttaagga atattgaacc agaaaaccga aaggcaggaa gtaaagttga aaaaacgaag
     8761 aattaaaaag aacctctttc ttatgttgct cggaacagcg tttaaaggcg caacttcttg
     8821 tttatttgcg cttttacttt taagcatcat tgaaaatgta agtgttagaa ataaagtttc
     8881 cgcaaaaaat aagttatttt ctgcagttgc aaagacttca agttctattg aagaaaaaac
     8941 tgcaaaatgg agataacttt tagatgcttt taatggggaa attatgcaat acctaacagc
     9001 aggagaaagc catggagaaa agctcgtcat tattattgac gatgtccctt gtggtgtttc
     9061 tttgtctgaa aaagaaatta attttgatct aaaacgccga agcagcggtc ttggaagatc
     9121 tgtccgacaa gacagcgagt ctaatgcttg caaaattgta tcaggagtta aggacggaaa
     9181 aacaacaggc aatccagttt gcattgaaat accaaactct aatgacaatc ctgaaagcaa
     9241 taacaacatt gttcgccctg gtcacgcaga cttaaatggc gttataaaat ataatttgga
     9301 tgactgtgaa aatgttgctg agcgtgcaag tgctcgtgaa accgctgcga gaattgttgc
     9361 tggcgttatc gcaaaaaata tgttggcaga atttgaagtc gaggtctatg gcttcgttac
     9421 ttcgattggt gctgttaaga ttaaacttga tgaaacaaat atgcctggga cattgccaga
     9481 tatgagtagt atcgcgatga gcgaggttat gtgtccggat gctaaggcga gcgagaaaat
     9541 tattaagcaa attgatcgag cccgcgaagc cggggaaagc cttggtggca gcattagact
     9601 tgtagctgtt ggtttagttc ctggtcttgg tggttattcc caaggatata gtaggcttga
     9661 tggcaagatt gcggctgctg tggcttcggt gccttcggta aaagaaattg aatttggaag
     9721 ctcggcttac gttagcgaaa acgttggaag cgttgggctt gataacattg ttaaagatgc
     9781 aaacggtgga tttgttcgcg aaacaaattt tgccggcggg cttgaaggtg gcatgacaaa
     9841 tggaatgcct cttgttgtta gcgcgaaggt tcgtccttgc ccgaccataa aaaagcctgt
     9901 taaaaccatt gatcttgaaa ctatggatgg tgttgttgtg gcaagcgaca agcgaagcga
     9961 tgtttgcgtt gttccaaatg ttgctgttgt ttgcgaaagt gagcttgcgt tgtgcctcgc
    10021 taatgagttc atggataagt ttggcagaga ttcgcttagc gacattaaat ttgccgttga
    10081 tgcttataag catagaatta aagagatgaa gtaggtttat atgtggggaa gtcaaagata
    10141 tttaattaga aagcctgttg cactaattgg ttttatgggt gctggcaaaa ccagcgttgc
    10201 gaagcttttg gaggaacaca ctggcattaa ggcagtggac acagacaatg ctgttgtcca
    10261 agcgacgcgt cagactgttt ctgaaattat taacaatcag ggcgaagaag agtttcgccg
    10321 agttgaggca aaagttttag gcgagcttgt cgaagagcaa aaacttatca ttgcaactgg
    10381 tggtggcatt attgaatctg ctgtttctcg aggcatttta aagcaatgtt attgtgtgtg
    10441 gttgaaggtt gatgcaaaaa attcggctaa gaggattgag gatttttcaa ctcgtccgat
    10501 gtttaaagat atcgaaaatg ctgaaaattt attggctcgt cgcaatgaga tttatgaaca
    10561 atatagcgat gttgccgtta atacaaacaa caaaccgcta agcgctgttg ttcgtgaggt
    10621 aagagacaac ctgctaaagg caggaatttt acaatattag gggcgaagac atggcaagca
    10681 aaagttcaaa taaaaaggta aaagttttat attccgcgtt tgaagctgtt ccatttatta
    10741 agcttggagg ccttggcgat gttgctggca cgcttcctgc ttcgataaat gttcataatt
    10801 gcgattgccg agtaatcatt ccttacttga gcatgatctc aaagcagcat caaatgctct
    10861 ttaaacacat tggcgattac agcgtttttt ctgctggcga ggagagggag tttggcctct
    10921 acttcgttaa acttcgtggt gttacttatt atcttattaa gaatgatcat tactttaatc
    10981 gtaaaagcgc atatggcgaa aaggatgata ttgagcgctt cgcttttttc agcagggcaa
    11041 ttttggagag cattaaacac atgaagggtt ttaagcctca agttttgcac tgtaacgatt
    11101 ggcacactgc gcttactaat ctatttttgc acgaagaatt cgaagacgat cttggcgctt
    11161 tgaagattaa gactgttttt acaattcaca atttgcaata tcaaggcaag tttaaatatg
    11221 aacaagtcaa agatgttttg aatttgaaga atcgtagcgc tgttgcgaag gtcatgcaca
    11281 agggcaaggt taatcttatg aagtgcggaa ttgtctccag cgattgcgtt accacaccaa
    11341 gcccttctta cgcgaaagaa attatgactg gcgaatacgg cgttgggctt gaaaatgttc
    11401 ttcggacgaa aaaagatgtt acccagggca tcctaaatgg tattgacact gttgatttta
    11461 accctgcttg ggataccaat attcgtgttc actttacaaa ggagttgatg aatcgtcgcc
    11521 ttggcaacaa gatcgatttg caaaagaagt tcaagcttga cgttgatgtt gatgctccgc
    11581 tattttgtgt ggcttgtcgt ttttatgaac aaaagggaat tgaccttatc attaaaggtg
    11641 ttccaaaaat catgaataaa ggcggacaaa ttgtttttct tggtgatggc gatgatgctt
    11701 acaagaaaaa gattcgtgat tataaaaaga aatataaagg cagagttgga atgcacttga
    11761 agtttgatcc tcagcttccg cacaatgttt atgcaggtgc agatgtcctt ttaatgcctt
    11821 cgaagtttga gccttgcgga attgcgcaaa tgatggctat gcattatggc acgcttccac
    11881 tggtccgaag cactggtggc cttcgtgata ctgttcatga ttacaaagca aagagcctaa
    11941 tttcgaacgg ttttgtcttt aagaattaca acttgaagga atttaatgca gcgcttgatg
    12001 ttgtctttga tgtttggaat aacgacaaga agaattgggc tcgcatgcag cgtcgcggta
    12061 tggaagagga tttcagctgg aaggaatctg gacgtaaata tagcgtgctt tatcgtgcgc
    12121 ttcttgatgg aaagattact aaagttgatg agatcttcga agctgagcaa gccaaacgtg
    12181 cagcgcaaaa ggctaaagag caggctgctc ttgccaagaa gaatgcgaaa gccagcatta
    12241 agatggctca aaaagttgcc gaagaagcat ccaactatga atttgtagat atttcccaaa
    12301 caaccgatga agattgataa aaaaaaggac gccgagtgac gtccttttta atttagctat
    12361 taattgaaga ttatttatct tcttttttct cctcttcttt aggagcttct tccttctttt
    12421 cttccttagg ttcttcagca ggtttttctt cttccttagg ctcttcctta gcttcctctt
    12481 ttgctggctc ttcagcaggt ttttcatcag ccggtttttc ctcagctggt ttttcctcag
    12541 ctggtttttc ttctgctggt ttttcttctg cttttggttc ttccttagga gcctcttctt
    12601 ttggagcttc ctctgctgga gcagcttctg gggctggagc agcttcgcct tcggctggtg
    12661 ctggagcatc ggctggagct tcggcgcctt cctcggctgg aggctcttct ttaggcttac
    12721 gctttgcagc agccttttct agctttagag ttgcttcgcg gcgcttggct ttttcttcgg
    12781 attctttctt cattgcagcc ttgcgctctt ccttagcctt agccttcttt gtgccacctt
    12841 tcttttcagg ggcgcgctta ggctctggct tgtattcctt gcgctctttg tcagttacga
    12901 tagagtttgc aagtgccatg atgccgcttt ttctgttagc agcttggttt ttgtgaatga
    12961 caccttttgc acatgctctg tctaaaagag tacaaacttc caacgcaaat ttataagcgt
    13021 cagcagcttt tttggcatct acagcatcct ttaccttgcg agtagctgtt ttgagttcag
    13081 ttttaatcgc aacgttgcgg acgtgtgcct tttcgtttgt tttaatgcgc tttttttgac
    13141 ttttaatgtt tgccattttt cctcctaaaa gttaaattcg ctaaatgcgc aagcacatac
    13201 tataacaaaa agtgctcgtt ttcgtgcaat tattttgatt ttttgaaatt ttagagtttg
    13261 aagttatagc cttttgataa aatagttcac gcttcttttg ggctgtagtt gagaaaagtt
    13321 ttcttttcaa gtccagggca gacgcggcca aaaggaccca cgtaaggcga aagcttatca
    13381 tggcgcgttc taggttgtaa gtcgcatcgc ttttaaggct tggccagggc gagagggcag
    13441 taaggaataa tccgaatgtt ccaatgcgcg agtgtggggg caaagactag gtcagcaaaa
    13501 gaagctttat tagtttggag gagatatgac taaaaaacga agtatttcaa ataaacttat
    13561 tgtttcatta attgcagctg caatggcgct tcttttgtgt gttgctgttg ctggttgtgc
    13621 accaagtaat tatgatcctc aacttaaaga cccacagcta aagactccag atattttgga
    13681 aaatggcaag ttgaaggttg gcgttgactt cggtaaccct ccttttgcgg gcgagacttc
    13741 aaaagctgct ggaatcgata ttgatgttgc ttcggcaatt gctcaagaac ttgggcttga
    13801 agttgaattc gtggatgttg ggacttcggc agatttggct ttgtctaaca aaaaatgcga
    13861 catggtaatg ggtgtttcga aggctgttaa cgttgaagaa atgtggaagt cagacgttta
    13921 tatcaagact gcatgcgccc tgttcgctaa ggacgaaaag gctgcagttc ctgtagctgg
    13981 aggcgctgat aaattcgcga ctgaaatggc ttctcaaagc gctattgctt gccagtctca
    14041 atttagcgcc gcaaacatta agcttaacgg cactttgtca gaatcgtttg agttgcttga
    14101 aaaaggcgat gttaaatatg ttgctgctga tgccgtaatt ggaacttata gcgttaataa
    14161 ttctgataac gaagcctacc tagttgctct tttatcaaag gtaagcggat attgtattgg
    14221 agctgacaag actaagacaa atcttactaa taaggtttca gcaatacttg gaaatttggc
    14281 aaaaaatgga gttttagaaa caatagaaaa gcgctggctt ggcaagacac ttgatcttga
    14341 taaggttcaa atgaccgctg gagctgcaaa tcaagcagat gcaggcgagc ttgaagccgg
    14401 tgtttattca caaactcatc caaataacaa cactccgtaa aatgtcgctt cagcatttct
    14461 ttttagagaa tcaagatttt ggctccgaag ttgaggcttc ggctggtggt agtgtgactc
    14521 taagtttaag tgcggacgac ttgcaccacg ctaaagttct tcgtttgaag tcgggcgagc
    14581 acatcggcat tattgactca aagcaaaaat ttttcgaagt agagattgtt gaattttcaa
    14641 aagatttgct tgttaaaaat tctacgaaga agacaaatac atctttgaac aactgtcatt
    14701 tatgactttg tgcaggcatt tcgaaaggaa acaaactgga cgatgttgtt cgtgcttgca
    14761 cagaaattgg agtttatggc tttattcctg tagattttgc aagaagcgta tctaagattg
    14821 atgcgaagaa ggaagcaact aaaaatgaac gttggcaaaa aattgcgaag agcgcagcaa
    14881 tgcaaagtgg tcaatttgca attccaaaaa ttagtctttc ggtaaatgtt tctaatttgt
    14941 gtgatcagct aagcgatttt gattgtgtgt ttatttgctg agaagaagct gaaagcatgc
    15001 aaaaaattgt tgaagtttgc aatgagctaa aagttaagga agaagagcaa aacatcgcaa
    15061 ttgtaattgg tccagaaggt ggcattacaa gtgacgaagt ccagcaactc aggaactcca
    15121 atagtaacgc tcacgttgta agcattggcg attcaattct taggactgaa acggccgggg
    15181 ttgtggcttc ggcaattatc aagtttaatt tgtcatgtta aaaaaatcga aaaaatttgc
    15241 tgttgtttct ttgggctgta aagttaatcg agctgaggtt gattcgtatt ctgcaaaatt
    15301 aattagtgat ggttgagaac taagtgacgt gcaagacgcc gatcttgtta ttgtcaacac
    15361 ttgcacggtt acctctgttg cggataaaaa gacaaggcag attgttcgaa aagtttgcag
    15421 ggacagcaaa gcgcaaaaaa tcattgtaac gggttgcgcg agtgcgatag agcctgaagt
    15481 ctatgagcaa attgattcgc gcatcgaaat tgttgcaaag gcagacatgc aaaacgccga
    15541 agcggacgcg ctgcgggttt tggcgggtga cgaattcagg agcagggcag cgcttaaaat
    15601 tcaagatgga tgcaacaacg catgcacgta ttgtattgtt cacactgcgc gaggtagtgc
    15661 ttgaagcatg cctcacaaac aggtcgaaga gcaggttaaa tgcatgcttg atgctggcat
    15721 taaagagatt gttttaagcg gtgtggatat tggcgcctat gatgataatg gttttggttt
    15781 gtcagatttg tgcaaaagcc ttcttgaaat tattggagac aggcttgcgc gaattcgaat
    15841 ttcaagcatt gagccaaaca acgttagcga tgaacttatc gaaattattg ctggcgcgga
    15901 cgggaaaatt tgcagacatt t
//
LOCUS       DBNCDC010000013        14983 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_1320_length_14983_cov_7.499397, whole genome shotgun sequence.
ACCESSION   DBNCDC010000013 DBNCDC010000000
VERSION     DBNCDC010000013.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 14983)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14983)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14983
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1320_length_14983_cov_7.499397"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            148..786
                     /locus_tag="AC04VQ_01900"
     CDS             148..786
                     /locus_tag="AC04VQ_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552982.1"
                     /translation="MLNSGKAFASPDSPRGEIPTSSNFMAEDDKVPFTFWQKTISVNG
                     QPMEETYTYVFEALTPGAPMPEGCVGDKYYFTMTDDQVLTFYIEYTYTGTFRYKSSMV
                     LPEPKPYYTYDETYFDVRVEASYDPGSTEKIHVYCYNPIGEKVYDPGWTITYNEPGPG
                     PGPEPEPEPTNPPQTFDANLIITAAAIGILLLCLLLAAIDRRKKNKVDEYKM"
     gene            complement(798..1697)
                     /locus_tag="AC04VQ_01905"
     CDS             complement(798..1697)
                     /locus_tag="AC04VQ_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIO9552983.1"
                     /translation="MLRVHKVPTDTIWRVFEWPGVLPYFNQAAQTGIGISWKSAVTAQ
                     MIGVVAYTIGEGVFTSKITLDSSTLVVWLIVVVVLGWACEKIVIAIIKNLTKRWELKG
                     VEIAERDPENEIVVDKDANLIISEITKIYNKKKVLDKFTMTVGAGERVALTGPTGSGK
                     TTLIRIILGIESCNHGEVFVKDYEPRTYSAVFQESTLLPNLSVDENIMVIANKYSNDE
                     LVPGNLMPDELSGGMKRCAEIERALLAQSQIVILDEPFAGLDSATKTKAIKYIDDNLK
                     GRSLILITHDKKDAKRLKCAISE"
     gene            complement(2131..3117)
                     /locus_tag="AC04VQ_01910"
     CDS             complement(2131..3117)
                     /locus_tag="AC04VQ_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIO9552984.1"
                     /translation="MPKTFKRTFAIVFAIAIAVCALAFMSSCEKSEKVCVGSLYGPTS
                     VSLLQLTGNENYELKAVAQTDALVADMASEKLDIALLPSNLAANMYQKMNGGVKVIDV
                     NTTGVLKFISQDASIDSLKDKTLYATGKGTVVQATLDIMLAASNMTERDINLQFKSDA
                     SEVIAYIKNDPSAVGLVNEPQASVALTEFSELHQIWDINDIWKGIFGPNSDILTAVTI
                     VRTKYLEENQAKVDRFIQDHKSSINFVNSRADVAVGIFNKFYANTGINATERSILNSN
                     LKFIEGEEMHQKLEEFLNKLYTYDHNLVGGQMPDEKFYYYAGQNLEEDVDNG"
     gene            complement(3117..3254)
                     /locus_tag="AC04VQ_01915"
     CDS             complement(3117..3254)
                     /locus_tag="AC04VQ_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012522.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0043448 - alkane catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HIO9552985.1"
                     /translation="MKYKCNLCGWIYDEEAEGTKFEDLPEDWTCPMCGAPKSEFTKLE
                     D"
     gene            complement(3265..4191)
                     /locus_tag="AC04VQ_01920"
     CDS             complement(3265..4191)
                     /locus_tag="AC04VQ_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015760474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufB/SufD family protein"
                     /protein_id="HIO9552986.1"
                     /translation="MSVDLSPIDKDLLENIAGIHGLPKGAFNVRKNGELVERHSSANV
                     EIETNDQGGLEVKIKDKAPRETIFVPVILTKVGMTDTVYNNFHIGKGASVEIIAGCGI
                     HNDEHKTTQHDGIHSFFCGEGSYTKYVEKHYGNGKADGSRVLNPQTKVVLEKNAVCEM
                     EMSQIGGVTSTIRNSDITIKEGAKLIITEKLMTEDKQTAESNMKIELVGDGSNVQVIS
                     RSVAKDESKQVFNPLVIGKCKCHGHVQCDAIIMGSASVKSVPAIEAASEDAQLIHEAA
                     IGKIAGDQLTKLMTLGLSEEEAENEILNDFLN"
     gene            complement(4199..4393)
                     /locus_tag="AC04VQ_01925"
     CDS             complement(4199..4393)
                     /locus_tag="AC04VQ_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552987.1"
                     /translation="MIISHQERILALADEIMLLDEGKITASGTPDEILPNLGFVAKDN
                     PDCQLSAPTALHGAKIATTC"
     gene            complement(4457..4972)
                     /locus_tag="AC04VQ_01930"
     CDS             complement(4457..4972)
                     /locus_tag="AC04VQ_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIO9552988.1"
                     /translation="MLELKNISFSVKDEATHKARKIIDDLSLKIDAGKLIVITGPNGS
                     GKSTLAKLIMGIEQPTSGQIFFEGKDITGKSLTDVANAGICYGFQQPARFKGMTIKKL
                     LDIAAGKRLPMLACNEYLAKVGLCSAGYLTREIDKSLSGGEIKRIEIATVLARDPKLA
                     IFDEPEAGIDL"
     gene            complement(4991..5284)
                     /locus_tag="AC04VQ_01935"
     CDS             complement(4991..5284)
                     /locus_tag="AC04VQ_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552989.1"
                     /translation="MDKQSLDNYIISSVAKIDAPSSALAKMQILDTYYFSDIDLSLIE
                     KNRNQIIHTTLDDVHEAGRVFAKLSKNSSKCVFGTKALNNCSDQFNIVDLFSV"
     gene            complement(5300..5701)
                     /locus_tag="AC04VQ_01940"
     CDS             complement(5300..5701)
                     /locus_tag="AC04VQ_01940"
                     /inference="COORDINATES: protein motif:HMM:NF046759.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552990.1"
                     /translation="MQDLSADVFNCKPIITYAGTNECISQITIGEKDAKAMANISIDE
                     ANDSAFIIPGDVNYCAMVNDLRLNNEKMDGTKVASSKIVALDYLWNNVRVKNGAYGAG
                     MIASPRGTIQFYSFRDPQIQKTFENFENSTK"
     gene            complement(5732..6868)
                     /locus_tag="AC04VQ_01945"
     CDS             complement(5732..6868)
                     /locus_tag="AC04VQ_01945"
                     /inference="COORDINATES: protein motif:HMM:NF019966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HIO9552991.1"
                     /translation="MDENFCADIDFDMLSEVYEPFIIVECKSSKKDASKNLLKLISDK
                     VTQIAEGGISKDMLQASISNVEFKLKEKLPDCTNGLDYSICAMSEWLYDGDASTSALK
                     YLDDIKWLKEQVKTDYFEKLAKEIFVANKHVAVVEDVPVEKTETKKPNYSDEEQKEII
                     ELCQNLVKWQASPDSKEDEAKIPVMSIDDIGEMPKMTKRTVEEKDITLVKYDINTNGI
                     TYINRYYPLNEFDGDDIHIISMLTELLGKLDTEKYSSEEIDVIAREKLGRLGFRCAFF
                     EKFDNPNNLDLRLTIRSAALDENSKYLATLIDEIIFRTKFEDSDKIFKILKQKQVSLE
                     QCFIAAGHSAALNNAASRILPVYKMSEMASGVAFYLYLKQILKT"
     gene            complement(7052..7873)
                     /locus_tag="AC04VQ_01950"
     CDS             complement(7052..7873)
                     /locus_tag="AC04VQ_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012879.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HIO9552992.1"
                     /translation="MPDNKIKIGEEIHGFKLLKTEDIPEIKFTGNIDDNGNHNAYEYV
                     HTSGAKLLHLENEDPELAFTIGFKTLPKDSTGVFHILEHSVLCGSKKFPVKEPFTNLL
                     KSSMQTFLNAMTFPDKTIYPVASTNEKDFYNLVDVYMDAVLYPNIYKNKNIFYQEGGH
                     KTPEGFSGVVFNEMSGVYSDPQEHIFEGLMQQLFPASCYAYSSGGAPEVIKKLTYEEY
                     LAQHNTNYCLANAYIIVYGNLDITKMFKFLNDNYLSKENELKEWRGSTPVTSQGK"
     gene            complement(7873..10233)
                     /locus_tag="AC04VQ_01955"
                     /pseudo
     CDS             complement(7873..10233)
                     /locus_tag="AC04VQ_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012563.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
     gene            complement(10319..11548)
                     /locus_tag="AC04VQ_01960"
                     /pseudo
     CDS             complement(10319..11548)
                     /locus_tag="AC04VQ_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014499.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
     gene            complement(11696..11797)
                     /locus_tag="AC04VQ_01965"
     CDS             complement(11696..11797)
                     /locus_tag="AC04VQ_01965"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIO9552993.1"
                     /translation="MPIFSLPSKTGIGTLGENAYEFIRFLKEAGQKY"
     gene            11892..12320
                     /locus_tag="AC04VQ_01970"
     CDS             11892..12320
                     /locus_tag="AC04VQ_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552994.1"
                     /translation="MKKFLNNQKAPVIACIIAIIVLVAVLVVLIATFSKASANLGIQQ
                     QWSSGKDSFDKAYEIAASDKETPDKTLYIPNLVEQLGKTVDQAVANIGQGATIVSSTT
                     SETVINLNNESGNDKAGTPSIIATTNRSHKITKISFSCNT"
     gene            12366..12518
                     /locus_tag="AC04VQ_01975"
     CDS             12366..12518
                     /locus_tag="AC04VQ_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552995.1"
                     /translation="MHIVEKTLTEAGLNVESGKAKVPDNRASWTTYDSDGTTVVMESC
                     DFSGSQ"
     gene            complement(12785..13267)
                     /locus_tag="AC04VQ_01980"
     CDS             complement(12785..13267)
                     /locus_tag="AC04VQ_01980"
                     /inference="COORDINATES: protein motif:HMM:NF014871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HIO9552996.1"
                     /translation="MLNGKDTKLQFASNTIYPDMSITQIPGDKANKEIKNGEFKMKEA
                     TDMFPDNYYLCAAAMTGAEIKEMLEYNAEHKYNIKMNELNQKTIVVDGEPEAAPITYG
                     LNFHYQMNMPVGHKAIIEGFANGKPFDMNGVYVVMVNDFIINSSATAIYDTIGWNRVI
                     "
     gene            complement(13409..13906)
                     /locus_tag="AC04VQ_01985"
     CDS             complement(13409..13906)
                     /locus_tag="AC04VQ_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552997.1"
                     /translation="MNEVGEDCDFIIVSMYNGFFYDDDYEYTSMNDKAYKTLIDRTNQ
                     PLVYGENTEAQAYRAVTNTTGVDMFICGADKKSFYSNMTFKNADESKDVLVVNGASEA
                     VTKSVFRAKYNKETEKYKIDLVTSENLNLNLYSPDQVLKNRIRPYVSDIENKLKNTPG
                     QIVGD"
     gene            complement(14000..14287)
                     /locus_tag="AC04VQ_01990"
     CDS             complement(14000..14287)
                     /locus_tag="AC04VQ_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552998.1"
                     /translation="MTDPKLTDIHSCAVAVDEIGYDGFSIGNHEYNYNYDVLDRNYNY
                     FKDKTTYICANLYDKVTGERMFKPYLTKSFKVDGDFLKVGIIGLECPNLTL"
     gene            complement(14447..14605)
                     /locus_tag="AC04VQ_01995"
     CDS             complement(14447..14605)
                     /locus_tag="AC04VQ_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9552999.1"
                     /translation="MKDTKSKRSSLIKIIALIVVCMAIGVFINNCSKEYTEDKFEILS
                     TTDMGGHI"
     gene            14654..14809
                     /locus_tag="AC04VQ_02000"
     CDS             14654..14809
                     /locus_tag="AC04VQ_02000"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: protein motif:HMM:NF012797.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0141098 - tRNA
                     (cytidine(34)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HIO9553000.1"
                     /translation="MLKIVLVEPEIPQNAGNIARTRACIGAQLHMVEPFGFRLSDKYF
                     TRSGCDY"
BASE COUNT         4348 a         3005 c         2728 g         4902 t
ORIGIN      
        1 ttctttgaca ggctgacaag aaaaagaaag agaagaagaa aaaggtaaaa gatattccag
       61 aaattaataa gtatggaacg tattagtaaa aaatattgta ttgctttatt attagctgtt
      121 ttttgcgctc tatgttgcat taatggagtg ttaaattcgg gaaaggcctt cgccagccct
      181 gattcacctc gtggagaaat tcctacatca agtaatttta tggctgagga cgataaggtc
      241 ccatttactt tttggcaaaa gaccataagc gttaacggtc agccaatgga agaaacctac
      301 acttatgttt ttgaggcttt gactccaggt gcccccatgc cagaaggttg tgttggcgac
      361 aaatattatt tcacaatgac cgacgatcaa gttttaactt tttatattga atacacctac
      421 acaggtactt ttagatacaa gtcaagcatg gtcttgccag aacctaaacc atattacact
      481 tatgatgaaa cttactttga tgttagggtc gaggcaagtt atgaccctgg ctcgactgaa
      541 aagattcacg tttattgtta taaccctatt ggcgagaagg tatatgaccc aggttggaca
      601 attacttata acgaacctgg cccaggacct ggacctgaac cagaacctga acctacaaac
      661 ccgcctcaaa cattcgatgc aaatttaatc attacagctg ccgctattgg cattctgctt
      721 ttgtgtttat tgcttgctgc tattgacagg cgcaagaaaa ataaagtaga tgaatataaa
      781 atgtagcaat tattttgcta ttcgcttatc gcgcacttca agcgcttcgc atctttttta
      841 tcatgagtaa ttaatattaa cgatcttcct tttagattat catcaatata tttaatggcc
      901 ttagtttttg ttgcgctatc aagcccagca aaaggttcat ctaatataac aatttgactt
      961 tgggctagca aagcgcgttc aatttcggcg catcgcttca ttccgccaga aagctcatca
     1021 ggcattaaat taccaggaac aagttcatcg tttgaatatt tattggcaat aaccataata
     1081 ttttcatcaa cactcaagtt tggaagcaat gttgactcct ggaaaactgc actatatgtt
     1141 cttggttcat aatccttaac gaagacctcg ccgtggttgc aagattcaat gccaagaata
     1201 attcgaataa gagttgtttt accagagcct gtaggacctg taagagcaac acgctcgcca
     1261 gctccaacgg tcattgtaaa cttatccaaa actttcttct tgttatagat tttagtaatt
     1321 tcagagatga ttaaattagc atccttatca acaacgattt cattttcagg gtcgcgctca
     1381 gcaatttcaa cacccttaag ttcccagcgc tttgtgaggt ttttgatgat agcaataaca
     1441 attttctcac atgcccaacc aagaactaca acaacaataa gccaaacaac aagtgtagat
     1501 gaatctaagg taatcttgga tgtgaacacg ccttcgccga ttgtataagc aacaacgcca
     1561 atcatttgcg ctgttacggc actcttccac gaaataccaa taccagtttg tgcggcttga
     1621 ttaaaatagg gaagaacgcc cggccattca aagactctcc aaattgtgtc agttggaacc
     1681 ttgtgaaccc taagcatatt agcgatgttc ttgtcgcggt tttggcatgc ttcggcaatt
     1741 gcgaagaaga aaattggcgt gcaagcgata ataaccgtga ttacatcaac tatgtctacg
     1801 ccaaatcaaa ccagcaacaa aacaataaaa caaacaattg gagcgctttt cataagttgg
     1861 ataattggac ttacaaaatc atgtatcaga gggattctat atcctataat tgcaagcaaa
     1921 cagccagcta cacatgcaat taaaaatcca ataagaattc taagtaggga accgccaatg
     1981 gttagccaaa atattggttc aaccatttta taaaacattg tgcgcaaaac atcatatggg
     2041 ccagggaaaa caacttggtt gttaaagatt agtgcgagta tgcctcatag acctacccaa
     2101 aatgatatta ccattatgat acgaattttc ttatccatta tctacgtcct cttctaagtt
     2161 ttgacccgca taatagtaga atttttcgtc tggcatttga ccaccaacaa ggttgtggtc
     2221 gtaagtatat aatttattga ggaattcttc aagtttttgg tgcatttcct cgccttcgat
     2281 aaatttcaag ttcgaattaa ggatgcttcg ctccgttgca ttaatgccag tgtttgcgta
     2341 gaacttatta aatatgccta ccgcaacatc ggccctggag tttacaaagt taattgacga
     2401 tttatgatct tggatgaagc ggtcaacctt cgcttggttt tcttccaaat actttgtgcg
     2461 gacaattgta accgcggtta aaatgtcgct gtttggaccg aagattccct tccaaatatc
     2521 gttgatgtcc caaatttggt gcagctcgct aaattctgta agggctacgc ttgcttgagg
     2581 ttcgttaacc aaaccaactg cacttgggtc atttttaata taggcaataa cttcggatgc
     2641 atcagattta aattgaaggt tgatgtcccg ctcggtcata ttgcttgcag ctaacataat
     2701 gtcaagggta gcttgaacaa cagtcccttt tccagttgca taaagcgttt tgtcctttaa
     2761 gctatcaatg cttgcgtcct ggctgataaa cttcaaaact ccagttgtgt ttacatcaat
     2821 caccttaacg ccgccattca ttttttgata catattcgca gcaaggtttg atggcaacag
     2881 cgcaatgtct aatttctccg aagccatgtc tgccaccagt gcatctgttt gcgcaactgc
     2941 ctttagttcg taattttcat ttccggtcag ctgcaacaag ctaaccgaag tagggccgta
     3001 cagagatcct acacaaacct tttcggattt ttcgcacgaa gacataaacg ccaacgcgca
     3061 cacagcaatg gcaattgcaa aaacaattgc aaatgttcgc ttaaaggttt ttggcattaa
     3121 tcctccagct tagtaaattc gctttttgga gcgccacaca ttgggcaagt ccaatcctca
     3181 ggaagatctt cgaactttgt tccttcagct tcttcgtcat aaatccaacc acataaattg
     3241 cacttgtact tcataattta cctcctaatt caaaaaatca ttaagtattt cattttcggc
     3301 ctcttcttca gatagaccga gagtcattaa ttttgtaagt tggtctcccg caattttgcc
     3361 gatggcagct tcgtgaatta gttgcgcgtc ttcgcttgcc gcctcaattg caggaacgct
     3421 tttaacgctt gcacttccca ttattatagc atcacattga acatgaccgt gacatttaca
     3481 tttgccaatt acaaggggat tgaaaacttg ttttgattca tcctttgcaa cgcttcgcga
     3541 aattacttga acgttactgc catctccaac aagttcaatt ttcatgttag actcagccgt
     3601 ttgcttgtct tcggtcataa gtttttcggt aatgataagc ttagcacctt ctttaattgt
     3661 gatatcgcta ttgcgaattg tgctggtaac gccaccaatt tggctcattt ccatctcaca
     3721 aaccgcattt ttctcaagca caactttagt ttgcgggttt agaactctgc ttccgtctgc
     3781 cttaccgttg ccgtaatgct tctcaacata tttggtatag gagccttcgc cacagaaaaa
     3841 gctgtggatg ccatcgtgct gagtggtttt gtgctcatcg ttatgaattc cgcaacctgc
     3901 aataatctca acgcttgcgc ctttgccaat atggaagttg ttataaactg tgtcggtcat
     3961 tccaactttt gtaagtatta caggcacaaa tatagtttca cgaggcgcct tatccttgat
     4021 tttaacctca aggccacctt ggtcatttgt ctcaatttca acattcgctg aagaatgacg
     4081 ctcgacaagt tcgccatttt tgcgaacatt aaatgcgcct ttaggaagcc cgtggatacc
     4141 cgcgatgttt tctaaaagat ctttatctat tggacttaaa tctacactca tggctttcct
     4201 aacacgttgt tgcaatcttt gctccatgca gggcagtagg agcactcaat tggcaatctg
     4261 ggttatcctt tgcaacaaaa ccaaggtttg gcaatatttc atctggcgtt ccacttgcgg
     4321 tgattttgcc ttcgtcaaga agcatgattt catcggcaag tgccaaaatg cgttcttgat
     4381 gtgaaattat cacgatggtt ttgtccttat gttcttcgtg tatagcagcg aaggtctgcg
     4441 ttagcctgtc aaaacttcac agatcgattc cagcttcggg ctcatcgaaa attgcaagct
     4501 tagggtcgcg cgccaagaca gttgcaattt caatacgttt gatctctcca ccactaagtg
     4561 atttatcaat ttcgcgagtc aagtaaccag cactgcaaag cccaaccttc gctaaatatt
     4621 cattgcaagc aagcattgga agacgcttac cagctgcaat atcaagcaat ttcttaattg
     4681 tcataccctt gaaacgggca ggttgttgaa aaccataaca aataccagcg tttgcaacat
     4741 cggttaagct tttgcctgta atgtccttgc cttcgaagaa aatctggcca cttgtaggct
     4801 gttcaattcc cataataagt tttgcaaggg tcgatttacc acttccattt gggcccgtga
     4861 ttacaattaa tttgccagca tcaattttta gcgacaaatc atcaataatc ttgcgagcct
     4921 tgtgcgtggc ttcgtcttta acagaaaagc ttatgttttt caactctaac attggaggaa
     4981 taattctaca ctaaacgctg aataagtcaa caatattgaa ctggtcagag caattattaa
     5041 gtgcctttgt tccaaacaca catttgcttg aatttttgct aagtttagca aatacgcgtc
     5101 cggcttcgtg aacatcatcc agtgttgtgt gaataatttg gtttctattt ttctcgatca
     5161 agctaagatc aatgtcagag aaataataag tgtccaaaat ttgcattttt gcaagtgccg
     5221 aagacggagc atcaatcttt gcgaccgaac ttattatgta gttgtccaaa ctttgtttat
     5281 ccaattcaag ttcgcttagt cattttgtgc tattttcaaa attttcaaat gtcttttgaa
     5341 tttgagggtc gcggaaactg tagaattgaa tagttccacg aggtgatgca atcattccag
     5401 caccataagc accattttta acacgaacat tattccacaa ataatctaaa gctacaattt
     5461 ttgagcttgc aacttttgtc ccatccatct tttcgttatt taagcgaaga tcatttacca
     5521 tcgcacaata gttgacatct ccagggatga tgaacgccga atcattagct tcgtcgattg
     5581 agatattcgc catagctttt gcatcttttt cgccgattgt aatttgagaa atacattcat
     5641 ttgttccagc atatgtaata attggcttgc aattaaatac atcggctgat aagtcttgca
     5701 attttttgat caaattatct ttagactttt ctcaagtttt aagaatttgc tttaaataaa
     5761 ggtaaaacgc aacgccactt gccatttcgc tcattttata aacaggcaaa atcctgcttg
     5821 cagcattatt tagtgcagca gaatgaccag cagcaataaa gcattgctct aagctaactt
     5881 gtttttgttt tagaattttg aaaattttat cgctgtcttc aaatttagtc cggaaaatga
     5941 tttcatctat aagcgttgcc aaatattttg aattttcatc taaagcagcg cttcgaattg
     6001 ttaaacgtag atctaaattg ttagggttat caaatttttc aaaaaatgcg cacctaaatc
     6061 ccaagcgccc aagcttttct cgagcaataa catctatttc ttcgcttgaa tatttttctg
     6121 tatcaagttt tcctaaaagt tcggtgagca tacttattat atgaatgtca tcaccgtcaa
     6181 attcatttag gggataatac ctgttgatat atgtaattcc gtttgtgttt atatcatatt
     6241 taacaagggt tatatccttt tcctcaacag ttcttttggt catttttggc atttccccaa
     6301 tgtcatcgat gctcatgacg gggatttttg cttcgtcttc tttggagtct ggacttgctt
     6361 gccactttac caagttttga caaagctcga ttatttcttt ttgctcttcg tctgaataat
     6421 taggcttctt tgtttcggtt ttttctacag gaacatcctc aacaaccgca acatgcttat
     6481 tcgcaacaaa aatttccttt gcaagctttt caaaataatc tgttttgact tgctccttta
     6541 gccacttaat gtcatctaaa tatttaagcg ccgaagtcga agcatcgccg tcatataacc
     6601 attcagacat agcgcaaatt gaatagtcaa gaccatttgt gcaatctgga agcttttcct
     6661 ttagcttgaa ttcaacattt gaaattgaag cttgaagcat gtctttcgaa attccgccct
     6721 cggcaatttg ggtgacctta tcagagatca actttaataa atttttggaa gcatctttct
     6781 ttgagctttt gcactcaaca ataatgaagg gctcatagac ttcgcttagc atgtcaaagt
     6841 caatgtcagc acagaaattt tcatccaaaa gtgccttctt taaaggactt tcgttagagc
     6901 ctgcaattgt gtttagaaga atgcgagttg cagctaattg cctgtggtct cttttgacaa
     6961 cgaagcccgc gccgcattct gcattttctt ttgcagttgc aacttctcta aagcatggtt
     7021 ttgaattagc ctcaaacgaa taattattgt ctcattttcc ttgcgaagtt acaggcgttg
     7081 agcctcgcca ttccttcaat tcattttctt ttgaaagata attgtcattt agaaacttaa
     7141 acatttttgt aatgtcaaga tttccataaa caataatata tgcattggcc aggcagtagt
     7201 ttgtgttgtg ctgggcgaga tattcttcgt aagttaattt tttaataact tcaggggctc
     7261 caccagaaga ataagcataa caagatgcag ggaaaagttg ctgcattaga ccttcgaaga
     7321 tatgctcttg aggatcagaa taaacgccac tcatctcgtt aaatacaact ccgctaaaac
     7381 cttccggcgt tttgtggccg ccttcttgat agaaaatatt tttatttttg taaatgttag
     7441 ggtagaggac ggcatccata taaacatcta ccaaattata gaaatctttt tcattggttg
     7501 aagccacagg gtaaattgtc ttgtccggga aagtcatcgc atttaaaaat gtttgcatag
     7561 atgacttaag caaatttgta aacggctctt taactgggaa tttcttagac ccacacaaaa
     7621 cgctgtgctc taagatatga aaaacaccag tgctgtcttt cgggagggtt ttaaaaccaa
     7681 tagtaaaggc aagctctggg tcttcgtttt ctaaatggag aagcttagcg cctgatgtgt
     7741 gaacatattc ataagcatta tgatttccat tatcatcgat attgcccgtg aatttaattt
     7801 cgggaatatc ttcagttttt agcagcttaa agccatgaat ttcttcgccg attttaattt
     7861 tgttgtcagg cattatttaa tcttctttgg gttaactgga tcaatatgcc aaatgttttc
     7921 agcgtattct ttaacacttc tgtccgaaga aaactttcca gcactgcaca tattcaagaa
     7981 ttgcttacga gcaaaagcgc gcatattctt cgcatcttca ttagccttaa gtttagcttc
     8041 caagcaactt ctaaaatctt ccagaacgaa gtatttgtct tcgtgcatca ttccaccata
     8101 aagcatttcg aagaatccgc ttccgtcatc gtcaagagtg ccgttaatca aggcatccaa
     8161 aacacgacga actcctttgg tttcgcgata atgttttctt gggttataag tatcacgaat
     8221 tttatccaaa tcttcaacgc gagcgccgaa gatataattg ttctcttctc cagcttcttc
     8281 gacaatttcg atgtttgctc cgtcataagt tcccagcgta acagcaccat ttagcatcaa
     8341 cttcatgttg ccagtgccgc ttgcttcggt gccagcggtc gaaatttgct cagaaatgtc
     8401 agcagcagcc ataatttttt ctgcataaga aacatcataa ttgctaacaa aaataacctt
     8461 tatatatttg ctaacaactg ggtcgttgtc aatcaaatcg gcaacagcgt taataacttt
     8521 gatgattgcc ttcgccttca tataacctgg agcgcttttg cctccaaaga taaatgtagt
     8581 agggacgaag tctgtcagct cgccttcctt aatcatgaag taaagctcaa ggatggtcaa
     8641 aatatttagg aattgacgct tgtattcatg catgcgctta acttgaacat caacaattgt
     8701 attggtatcg aagctcttgc catccttctt ttgaatgtat ttgttaagtt cttctttgtt
     8761 atgagcttta atttcaatga acttgtctat cactttatca tcatcggcat atttctttaa
     8821 gccttcaagc aattcaagat cgctaagcca cttgtcgctg ccaataagtt ttgtaattaa
     8881 cttgctaagt tcagggttgc aaacgccaag ccatcttcgt tgagtaattc cgtttgtaac
     8941 gttaatgaac ttcttcggga aaattttata gaaagttgaa agcacagtgc tcttcagcaa
     9001 attagtgtga agctgagcaa caccgttaat gtggcttgaa catcagcaag ccatatgcgc
     9061 catatgaacg gtagagcctt gaagaatttt gacgtttgcc tgctcttttg acgtaagttt
     9121 tttaaattcc ttagtttcgc aaaaatagtc agaaatcatt ttaataattc ttgcaatatc
     9181 gtgagaaaca tgacggacaa acttcatgtc ccacttctca agcgcttcag ccataattgt
     9241 gtggtttgtg taattaaata ccttgcacgc aatttttagt gcaggttcaa attcaactcc
     9301 acgctcacca agcaggcgaa taagctccgg aattgaaatt gttgggtgag tgtcattcaa
     9361 ctgaatttga ataaagtcag gaagtttctt tcaattttgt ccatgctcct gttcgaattt
     9421 tttcaaaatg tcttgcaaac ttgcagagca gaaaaaatat tgctgagtca aacgaagctg
     9481 acggccagca tcgcttgagt cgttagggta gagcacgcga gaaatatttt cagtgtgggt
     9541 tttctcgctt actgcttcat cgtatcttcc ctggtcaaac aaggcaaaat caaattcgtg
     9601 aacaggttca gcctgccaca aacgaagcgt agccaagtga gttgtgccat agccaataat
     9661 tggcatatcg taaggcacgg cataaacatc accatgtcga agatgaactg taacggtgtc
     9721 aactgattga cgaacactcc aagggtcgcc taatcttcgc cagttatcaa tttcttcgtg
     9781 ttgaaaaccc ttatcaaaag tttgcttgaa aagaccataa ccatagcgaa ttccataacc
     9841 agttagggga taattgcaag ttgctgcact atccaaatag caagctgcca aacggccaag
     9901 gcctccatta ccaagggccg cgtcttcgat ctcttcgaag caatcgatgt tgattttttc
     9961 acggttcaaa attacttttg tagcatttag aaggcccgag caaaaaaggt tgttataaat
    10021 tgcacgaccc atcaaaaatt cagcagaaaa ataacaagct cttttatttt tgatgtgcaa
    10081 ttcttccgaa gcaagccaat ttggcgtgat agattcaatt acagcttttg aaataacatt
    10141 atgaagctgg ccaactttta ggctttttaa atcatattga tagattgttt ttcgaataac
    10201 ttcaataact ttgcgaaaat cagtagttgc cattttaaaa cctttcgcta ttttttgtca
    10261 tttctaacaa ttttttggca tccaagctgc taaattgatc gtagctcatc tgccattttc
    10321 aattaattcc tgttgtgccc ggagtattta tgcgggcatc ttgcgccaat tctaacaaat
    10381 cttgcaccat caagatacaa atatttgcgt cactttgaac aatgtcgttc atcattatat
    10441 gtaacaattt atgggggtcg gtttcttcgt agaaattctc aaaattcttg cgaacttccg
    10501 gaagcgcttc gtcgatatat ccaataattg tatttgtatc atgcgttcca agataggcga
    10561 caaacggctt ttcataattt tgaggcaagt atttgttctt gtagcttttg cgagaatcgc
    10621 caatgtattg atcagcgtgc tttgtttcgt taaatgcgta ttgcaagacc ttcatgcctt
    10681 cgaagttgca atcgttaaca agctttttga gctcaggcgt ataaatgcca aggtcttcag
    10741 caaatattgg catgtcagag acactattaa aaccaagctg atccgctgcg gtttgccaaa
    10801 attcaaaacc agggcctttc ttccaaactc cagattttgg gtcacgtgtc tcagcagaaa
    10861 cgttgtagta tttttcaaaa cccttgaagt ggtcaaggcg aacaatgtca tacaaatcta
    10921 aagcattgcg aagacgattt atccaccatc tataatttgt tgacttcaaa tattcccaat
    10981 cataaactgg gctgtctcaa atttgcccct taccttgctt gttatgagcc ttgtttggtg
    11041 ggcatccaga ataaacagag accgtgaaat catcgttgat gtcaaaaata tgaggatcaa
    11101 cccaggcatc ggcgcttgtt agagcaacat ataggggaat gtctccaata atctttatgt
    11161 cattttcatg agcgaacttt tgaagacttc gtcattgtgt gtagaaaaag tattgaagca
    11221 ttttgtgata gttaagttcg tcttcgtgat cttttgcata agcgtttagc gcttctttgt
    11281 cgcgatattt ataaatatca ggtcattcgt gaagttctgc tccgccaaat tcctttaaca
    11341 aagtcataaa caaagcataa tcatttagcc aatcttcatg ttcttcgcaa aatgtcgaat
    11401 aatccgccgg aagcttttta aagaagttta caaataattt gccgaacaat ttttcacgtg
    11461 attctttgac ttgatcatag tcaatttttg cacttggcaa tttgctgccg aagcgttttg
    11521 cctcgtcttc ggcaagtgga gcaggcataa tattggcgtc ttggacatct tcgtttgtta
    11581 atcaaccatc tccaacaagt caatctatgt caattaaatt agggtttcct gctcgcgaag
    11641 aataacttag ataaggggaa tcggcttttg aaggagggca aattggcaat atctgtcagt
    11701 atttttggcc cgcttctttt aaaaatcgaa taaattcata ggcattttct ccaagagttc
    11761 caattcctgt tttacttgga agtgaaaaaa ttggcataag tatgcctgaa gttctatttt
    11821 taaacgctgt aatgccttct ccttgtcttt taactgcaca aattataatt taatttagta
    11881 taattgcacc tatgaaaaaa tttttgaaca atcaaaaggc ccctgttatt gcttgtatta
    11941 ttgcaattat tgtacttgtt gctgttcttg tggtgctgat agccacgttt tcgaaagctt
    12001 cggcaaattt gggaattcag caacaatggt caagcgggaa agatagcttc gataaggcat
    12061 atgaaattgc ggcttccgac aaagaaacgc cggataaaac tttatacatt ccaaatttag
    12121 ttgagcaatt ggggaaaact gttgaccaag cagttgcaaa tatcggacag ggagcaacaa
    12181 ttgtttcttc aactacaagc gaaacggtta ttaatttaaa taacgaatcc ggaaatgaca
    12241 aggcaggcac tccaagtatt attgcaacaa ctaatagatc tcataaaatt acaaagatat
    12301 cttttagctg caacacctga ctcttaggat atgcaaatta cagttttgta gactacgttg
    12361 ataatttgca cattgttgag aaaactctaa ccgaagcagg cttaaatgtt gaaagtggaa
    12421 aagctaaagt tccagataat agggcaagct ggacaactta tgacagcgat ggaactaccg
    12481 tagtcatgga gtcatgcgat ttttctggca gccaatgaca aaatggaaga aactataaat
    12541 ggagttcaaa cttaaacttc aattattcgc tcgccaacgc aaaaggcgac ttgtctgaaa
    12601 caattaggct aattacaatt actattgaac aaaattagta ttcgatgtat cactttgaga
    12661 cgtattcgtt attcttaagt gcatctttgc ttggataaac cccaccatat tttgatgtca
    12721 tatcacttgc atagaggtct attatgcttc gaataaaaga ttcgttattg tttaattctt
    12781 gatctcaaat tacacgattc cagccaatag tgtcatatat cgcagtggct gaactattaa
    12841 taatgaagtc atttaccata acaacatata caccattcat atcaaatggt tttccgtttg
    12901 caaaaccttc aattattgcc ttgtgaccaa ctggcatgtt catttgatag tggaaattaa
    12961 gtccatatgt tattggagca gcctcaggtt cgccatctac aacaattgtt ttttggttta
    13021 gttcattcat tttaatgttg tatttgtgtt cagcattata ttcaagcatt tctttaatct
    13081 ctgcgcctgt cattgccgca gcgcatagat aataattgtc tgggaacatg tcggtggctt
    13141 ctttcatctt gaactcacca tttttaattt ctttattagc tttgtctcca ggtatttgag
    13201 taatagacat atctggatat attgtattcg aagcgaattg taacttcgtg tcctttccat
    13261 ttaacaattt gagctttaaa ttaatgtcat ctacacttct gcttgagtgg ttggcaattt
    13321 tataaccagc tcaaatttgt gcacgattta taaagtccgc atagtcagtt tgctttgtgt
    13381 agtaatccat gtattcatgt tcgttgtttc aatctccaac aatttgacca ggtgtattct
    13441 taagcttatt ttcaatgtca cttacatatg gcctaatacg atttttaaga acctgatcag
    13501 gagaatataa attcaaattc aagttttcag atgttactaa atcgatcttg tatttttcag
    13561 tctccttgtt atattttgct ctaaagacag attttgttac tgcttccgaa gcaccattaa
    13621 caaccaggac gtccttgctt tcgtcagcat ttttaaaagt catgtttgaa tagaaggatt
    13681 ttttatcagc accacaaata aacatatcta caccagttgt gttagttaca gcacgataag
    13741 cctgagcttc ggtgttttct ccatatacaa gtggttgatt agttcgatca attaatgtct
    13801 tataagcttt gtcattcatt gatgtatatt cgtaatcgtc atcgtagaaa aatccgttat
    13861 acattgaaac gatgataaag tcacaatctt cgccaacttc gttcatttct ttttgaactt
    13921 tagtaatttc atatgcaagg tcaccgttgg tgttttcagg tgagtgggca attaagtttg
    13981 gatattctga actggcatct caaagagtta gatttggaca ttctaaacca ataattccaa
    14041 ctttcaaaaa gtcaccatct accttaaatg attttgtgag ataaggctta aacattctct
    14101 caccggtaac tttatcgtat aagtttgcac atatataagt agttttgtct ttaaaatagt
    14161 tgtagtttct atccaggaca tcgtagttgt aattgtattc gtgattacca attgaaaagc
    14221 catcatatcc aatttcgtca acagcaacag cacaagaatg aatgtcggta agttttggat
    14281 ctgtcacctt agcaagtcca aggctttcaa tcaatgtatc ttgccataaa ttaccactgt
    14341 ctcatgtgat tgttctgtcg tcataatctt gacgagaatt tttaattgca gtacttgctg
    14401 ccaatatgtt attaggaaaa ttttccccgg ttagtatatt ttgatctcaa atatgtccgc
    14461 ccatatcggt agttgataaa atttcgaatt tgtcttcggt atattctttt gagcaattgt
    14521 tgataaatac accaatagcc atgcaaacaa caattagagc aataattttt atcaaagacg
    14581 atcttttaga ttttgtgtct ttcatgctaa cctccataga ttcttatatt ataatgtaat
    14641 cgagggtgaa attatgttaa aaattgtatt agttgaacca gagattccac aaaatgctgg
    14701 caatattgca cggactcgtg cctgcattgg agctcaactt catatggtag agccttttgg
    14761 ttttagactt agtgataaat attttacgcg tagcggttgt gactattgag ataaggttga
    14821 gctaattcgc tggcctagtc ttgataaatt tttggaggaa aatcaaaaca aagatatgtg
    14881 attttttact gggcgtgttg aaaaatcttt ccgagatgct aagttcaccg aaaattcatg
    14941 attagttttt ggaagagaaa ctgcaggtct tcctccaaag ctt
//
LOCUS       DBNCDC010000014        14946 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_1332_length_14946_cov_7.459741, whole genome shotgun sequence.
ACCESSION   DBNCDC010000014 DBNCDC010000000
VERSION     DBNCDC010000014.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 14946)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14946)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14946
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1332_length_14946_cov_7.459741"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(35..304)
                     /locus_tag="AC04VQ_02005"
     CDS             complement(35..304)
                     /locus_tag="AC04VQ_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553001.1"
                     /translation="MFTENDNPYKRDYRNLNHLFYQSAYDTYGYYYFDSGGMTTTFDS
                     GHFAQLRKKADNVGSATGEGTDCYDTGSDFVLFTNPSTDEVKFAP"
     gene            complement(1400..1840)
                     /locus_tag="AC04VQ_02010"
     CDS             complement(1400..1840)
                     /locus_tag="AC04VQ_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553002.1"
                     /translation="MFVLSAFMVFLSVTAAMGYGTSEPLINKLQTTQTETTQTQTDDT
                     ETQTAEVEDEPAKLTETQFKINSEISNPNYVSTYPVNFSYTGQTTRTSTIHYGYLDTE
                     DNFIDIPAFQTASVEKDYSRLTASGQGYIFQDGAAQATTVSDFQ"
     gene            complement(2135..3058)
                     /locus_tag="AC04VQ_02015"
     CDS             complement(2135..3058)
                     /locus_tag="AC04VQ_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014680.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIO9553003.1"
                     /translation="MEEEMVEILCFVIIALLIIAILILVFKKPKTGTDQLTKAAVEQN
                     VKMDAVSKQLDNVFERIIHMERISDEVDNLSKVLTNVGTRGAFGEAQLGRMLEQTIPG
                     MFVRNYKPNPRSTDHVEYAIKIPNGKNKDFTYLPVDSKFPMDKYSVLVQASEGNNPDE
                     VEAARKDLITFIDRSATTIKNKYIRVPQTTNFAIMFLPTEGLYLEAVKDPGNIQYKLQ
                     ERGIMIAGPSTILALLNSLSMGFNMIEINESADEIRKMLGNIKQQFDKFNKNFETIES
                     GIATASRGLADAKDRSRQINNALDRVEITED"
     gene            3144..3584
                     /locus_tag="AC04VQ_02020"
     CDS             3144..3584
                     /locus_tag="AC04VQ_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553004.1"
                     /translation="MGAQSILTTSIGVIASQNATTGTSDNLLFIAIPIIAALIALALS
                     PFAFKRIHSNHGASVIEVRKITLVSIASVLISALCILFFLSNAQPASAANGSISINDH
                     IDLVLDENNEVASADAKITNGTGEIYSLDTIRVISNNESIGDDI"
     gene            3660..4196
                     /locus_tag="AC04VQ_02025"
     CDS             3660..4196
                     /locus_tag="AC04VQ_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553005.1"
                     /translation="MQPNQSATVRISLQLSGDEENILSGNIASLEIATKPTKVSVYYT
                     NNDEKKSNNINFNFDFAEFENADNTVVNRKLATVASLLDTKMYEAEQIVVEGQTYDKT
                     KKTALEKALGFEDNYYYRMYDGETQSDPGVDIVKPDLSTEKNDTTSYEFAHKKIFFGD
                     HYSEIVSVNIAGTQCSEE"
     gene            4278..4805
                     /locus_tag="AC04VQ_02030"
     CDS             4278..4805
                     /locus_tag="AC04VQ_02030"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HIO9553006.1"
                     /translation="MHKGFEIAANRVEYALNDYLNKYVQDDANTNRILFISGHSRGAG
                     VANIVGRDFEYLANAKTFTYTFAAPNTTDDIDSKLNYKTIFNIMNMDDIVCQIPCTNM
                     GYDKYGRDVCLSISTGKSITGGDLTDAFESIMMDDEYGGNTPTEIFNLTELLRNNIVK
                     GTREQCYEIGEDQTI"
     gene            complement(5159..5392)
                     /locus_tag="AC04VQ_02035"
     CDS             complement(5159..5392)
                     /locus_tag="AC04VQ_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553007.1"
                     /translation="MLNAYTRASKEVCDIDVIVNGTHGGLEVGIFLDKRPSMQCISFG
                     ADVKGEHSTNETFYTKSLPYHFATLIRTLETLN"
     gene            complement(5414..6682)
                     /locus_tag="AC04VQ_02040"
     CDS             complement(5414..6682)
                     /locus_tag="AC04VQ_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding metallopeptidase family protein"
                     /protein_id="HIO9553008.1"
                     /translation="MANSVVIDEYPNTVAQNATINENYKEAANKVAESAIADFLDLCK
                     VVRTTADCTHISEHLANWAKTHNLNPVVDESLNVYWDVPASPGCENWPKIIFQTHQDM
                     VWETKNSASPDPKPVDAVYDEETGAIHSRNFETTLGADPGEGIITLLAISKDYGFKHG
                     PLRVIFTTKEETTMDGAYGLSSEVLDSDFMVNIDGRQAGIVRIFSCGVRTFVYKKNYE
                     MTKSEHDTQIKINVDGLLGGHSANSIIYKRVSAIDVLRNVMNRLVERNINFDLISLEG
                     GQNFNAIAYSAEMKLACSKNDVDAIKEICNSVIDDQMANSVDDKNGKCNFEVSDLTSE
                     VFNSEATKQITELLNAFHNELFDMFEEVELVPKNSLNMGVVNIKDGTLDFTLMYRFGE
                     EEFLKKLEKFNDEVSKKLQINYDVIETLPA"
     gene            complement(6685..7005)
                     /locus_tag="AC04VQ_02045"
     CDS             complement(6685..7005)
                     /locus_tag="AC04VQ_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553009.1"
                     /translation="MLEICSIVMPIFYICVFVAYIALKVDKKKVKKHKDDFIFIKNKG
                     LGIFVGLWCAVITFAGMIMKMQNDDPAKFIMNVAIPIVLIAMGLILPLIAKAYNNKKG
                     VKTK"
     gene            complement(7006..7947)
                     /locus_tag="AC04VQ_02050"
     CDS             complement(7006..7947)
                     /locus_tag="AC04VQ_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIO9553010.1"
                     /translation="MGWVVILPYLAQKPSLIIVAFNWMVNQNGDISSIDPMTVQLIGV
                     VIFLVCVFLASRGIKVVKTIASIAGTGSFIMAMLFIVFGLLAPIFNVNPSEIPTYSLN
                     LDPTSFVPTDMSVFASLSILLFGVGGAEKIAPYVNRMEKPGRDFPKGIIAVVIMVAVA
                     AFAGAIAMGLMFGQDCQGMIADPNNLDPNKTYTPMQIVANGESTTVLMDPNFITNGQY
                     ICFQKVGQAFGLGNVLMVLYAAIKLITDCAVLIVSIDVPLRLLLEDSDQRFIPKGSLK
                     KNKYGAYPYWLLVVTVITVFLLIVPSLAMGTADDLIK"
     gene            complement(7993..8172)
                     /locus_tag="AC04VQ_02055"
     CDS             complement(7993..8172)
                     /locus_tag="AC04VQ_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553011.1"
                     /translation="MTFCTLWGFNNLINGCFYFGQTRVMSVWFVLFLLYFLPYSLMVG
                     ELGSVFRSHGGGVSS"
     gene            complement(8308..9147)
                     /locus_tag="AC04VQ_02060"
                     /pseudo
     CDS             complement(8308..9147)
                     /locus_tag="AC04VQ_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014881.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
     gene            complement(9148..10848)
                     /locus_tag="AC04VQ_02065"
     CDS             complement(9148..10848)
                     /locus_tag="AC04VQ_02065"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013978815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIO9553012.1"
                     /translation="MKSDIEIAQETPMNDIREVAKEAGIAEDSLELYGNYKAKVNIHK
                     VKANKNGKVILVTALTPTPAGEGKTTISVGLADSMRAYLKDSDKNVMLVLREPSLGPV
                     FGIKGGAAGGGYSQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIQQGNDLGIDPK
                     SITWRRAVDMNDRQLRNIVDGLGGKMQGVPREDGFDITVASEIMAVFCLATDLNDLKK
                     RLGNIVVGYTYDEKPVTASDLKAQGAMTALLKDAIKPNLVQTLEHTPAFVHGGPFANI
                     AHGCNSVIATKTATQLADYVVTEAGFGADLGAEKFLDIKCRMSGIRPSAVVMVATVRA
                     LKLHGGAAAGMKAAEMKEELKKEDLKALEAGFPNLLQHVKNIEDVYKLPCLAVINVFP
                     TDTDKEIELLYKLCKDANVNVQVSHTCMNGSKGSEDLAQAAIDLCEQDSSKFAFSYDL
                     DKGIAEKLNDLATKIYHADGVEFIGNAVKQLPVLEKQGFGKLPVCVAKTQYSFSDDPN
                     KRGVPKDFKITVRNLKLAAGSGFIVALTGEIMTMPGLPKVPAAEKIDVSEDGVISGLF
                     "
     gene            complement(10861..11157)
                     /locus_tag="AC04VQ_02070"
     CDS             complement(10861..11157)
                     /locus_tag="AC04VQ_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553013.1"
                     /translation="MFEQAAVVASAATMLTAIDIFAELGLLNVETVARDGQTYSKIAL
                     VDVSSKVNLEDSSRFCEGRNEIENFEDYRITAMRKTREELTKLLRHPILPGESI"
     gene            complement(11185..14199)
                     /gene="recJ"
                     /locus_tag="AC04VQ_02075"
     CDS             complement(11185..14199)
                     /gene="recJ"
                     /locus_tag="AC04VQ_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIO9553014.1"
                     /translation="MEISPLLARMLEVRGITDQTEIEKFLKPSLERDWGNPYDIKGME
                     QVADAVENAVRAGHRILIYGDYDLDGISATVVMMRGLSAIDKHINAGMTVDYFLPKRF
                     SEGYSLSEDSVNRLLEMDPLPQTIITVDCGITSAPYIETLKSHGIDVVVTDHHEKSGD
                     IPVGIAMADPEAEDGDSPDKILAGVGVALKLIQSLGGRLGLPHLWMDFIDLATLGTIA
                     DVMPLIGQNRALVTQGLEMINKQPRPSLRAILDVAGRKAGSMTAKDVSYVLTPRLNAA
                     GRMDEPEKAVDLLLCDDYAQAYNIAIEIEAFNNQRREITEKMLEQAEFRAQKIIEDNP
                     GKRSLVLSDEAWHPGVLGIVSAKLAESYNVPTLVMSVQDGVAHGSGRSVGNVDLHEAC
                     SKSFDKFIKFGGHKGACGATLKAEDVAEFSEKLEGYMQSLDISEFQKVLKSDLELRLH
                     ELNVEDIESLNVMEPFGNANEEPKFITSNVFIRRAKAVGQNHNHLSCNITDGAYGGMG
                     IMFNVSNIDKYLALDSTVSVIYTPKVEEFQGRKNVKMHIDHIIPTDELHNTEDTNAGG
                     SFVSQLFDKFEVKEKNLVSVGDFIKDSVRELSSDMMQRSAQNQSAENLNEAQDFRDKK
                     IDEVQQLIVEAMIGKDGKLHQSQQETLDLLAKGKSVLSVMPTGRGKSLIFQVFAAAQA
                     VCNKKMSIFVYPLRALISDQAFHLQKNLKDFGLNIRVMNGETPADEREENYKLMSEGK
                     LDIMLTTPEFLAIHIDKIAQHAKIGFIVIDESHHMGQVKIGERTAYGHMPKIIEKLNH
                     PQVLALSATVNDDICNEIKKFLPIEEVVKDNFERENLFVDDRRNIKSRDDYLVALCAS
                     GEKIVIYVNSRQQTVDLARLIKSRAPHIAMKVGFYNAGMSREDRNKIETLFRENELSV
                     LVATSAFGEGVNIPDIRHVCLYHLPFSNVEFNQMAGRCGRDNNPAIVHLLYNRADATI
                     NTQILEMTNPTEENLRGIYAAIRENVKNNNGT"
     gene            complement(14263..>14946)
                     /locus_tag="AC04VQ_02080"
     CDS             complement(14263..>14946)
                     /locus_tag="AC04VQ_02080"
                     /inference="COORDINATES: protein motif:HMM:NF044297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553015.1"
                     /translation="TQETTFPSQTSTIKVGGSGSFVMKLRVPAWATSGFSVTKNGVAV
                     SAEAKPSSYVSVSVSAGDTIVVNMPWTYHVELLPDKLDGSYVGSLMYGPFVMAAQNDS
                     RTYLNLNLQNDIAKSVENIGLDSRGLPILKTNGYTFYAMPSTEIAHIPYHAYSKITLL
                     DNEGENVTDFFYSSLTPFSPEPSPTPDPEIVTPTPQTGDVSALFALAVLSIAVIGAYV
                     FTRNRFNNN"
BASE COUNT         4467 a         3195 c         2807 g         4477 t
ORIGIN      
        1 tacggttatc tgcaactgcg ttatctttat cgtttcaagg cgcaaacttt acttcgtcag
       61 tacttggatt ggtaaacaaa acaaagtcgc ttccagtgtc ataacaatca gttccctcgc
      121 ctgttgcaga gccaacgttg tcagcctttt tgcgaagctg agcaaagtgt ccggaatcaa
      181 aggtcgttgt cataccacca ctatcaaaat aataatatcc ataagtatca taagcagact
      241 ggtagaagag gtgatttagg tttcgataat ctcgcttata aggattatca ttttcagtaa
      301 acaaataact taaatctgtc gtagatttat ttcaaatact atctggatta agctgagggt
      361 aaccattggc tcctaatgta ttagctcaaa tgccgcgtct tgtatataaa tctcgccccg
      421 taccacctgt aacagttctg tttatgtttc tctcgtctgt atgagtctgt tgatcttctc
      481 aatcttcaac ttcaccacca ttaccatttc ttgcacttga aaatttaaaa tcactctgaa
      541 ggtttattgc attgttatat ctatatggtt gacgatttcc ggaagttgta gcagtaaggt
      601 ctctccagcc atcatagtcg aaacagttga tagtaatatt ttgggaagca ctatcaacag
      661 ttggtgtaac cgactcactt gaataatcat atgtaattga aacacaaact ggtgttgacg
      721 ccgatcctag ctctattcat gtaccattac cattatcgta atatcatgta ctgtttgcac
      781 acgtaagacg agttgctata tgatattctg gatcggcccc ttctgcaggc atcaaatata
      841 tgtaagtaat gtaataaaca caatcataag ttcctatgcc tggctctact tgtcgaacat
      901 accttccaca ataattttta aatgtcatag gagaagcact tgttgtactt gatgaatgtt
      961 ctaaattcaa atcaccagca aaaggtggat ctaatttata ataattttga gcagtaatcg
     1021 gaatacttgc atcagtatag caatactgac aatatgcagt tggtggcttt tcggcgccat
     1081 tatctcaaac tgctttaaaa tcaacctgat ctagggcttc tgtcctgcct tttcttgtaa
     1141 catctttgaa aacaactcaa acatcagttg cattttgtcc agctgcagaa tacaaatgtc
     1201 atggagcagc agaagtttca gaaccaagat cgtttcgata aaatcatcca taattgttac
     1261 cataacgcgc atatgtaagc tttgttagct gcacggcatt tgcagcagta gcatcagaac
     1321 ctaaatagca atcacaaaaa gcataagtat gaccatcata tgtaaatgtt tcttcgtaat
     1381 aacgaacgtc tcaatgactt cactgaaaat ctgaaacggt tgtagcttga gctgctccat
     1441 cttggaaaat ataaccttga ccagaagcag ttagtctgga ataatctttt tcgacagatg
     1501 cggtttgaaa tgccggtatg tcaataaagt tgtcttctgt atccaagtaa ccatagtgaa
     1561 ttgtagaggt acgtgtggtt tgtccagtat atgaaaagtt aactggataa gtgcttacgt
     1621 agtttggatt tgaaatttca ctgtttattt taaattgagt ttctgtcaat tttgcgggct
     1681 cgtcctcaac ttcggcagtt tgagtttcgg tatcatcagt ttgggtttgg gtggtttcgg
     1741 tttgggtggt ttggagttta tttatgagtg gttcagatgt gccatagccc atcgcggccg
     1801 tgacggacaa aaataccata aaggcactaa ggacaaacat gccaaagaca aaacgaattt
     1861 tgctttgggc attttcgcca aagtgtagaa aagaactatt atttttagta aaacccctca
     1921 taaaactaaa aatttaatag aacataatta cgtttttgct acttttttat tctattttgt
     1981 gtacaccacc tccaaattat atacacccgt ataatattat accttttttc ccaggtcaaa
     2041 gaccaaaaat tataatttag tgatgcgcgt atataaacgt tttaaaaata gaaatatgat
     2101 gaaaatatta gattttttga agtaggagaa attgttaatc ttcggtgatt tctaccctat
     2161 ctaaagcatt atttatttgc ctgcttctgt cttttgcatc agctaaaccc ctggaagctg
     2221 ttgcgatgcc gctttcaatt gtttcgaaat tcttattaaa cttatcgaat tgttgcttaa
     2281 tatttccaag catcttgcga atttcatccg cgctttcatt aatttcaatc atattaaagc
     2341 ccattgaaag cgaatttagc aatgccaaaa ttgtgcttgg accagcaatc atgatgcctc
     2401 gttcttgaag tttatattga atgtttcctg gatcttttac agcttcaaga tataaacctt
     2461 cggtaggtaa aaacataatt gcaaaattag tggtttgagg aactcgtata tatttattct
     2521 ttattgttgt agcgcttctg tcaatgaagg ttattaaatc cttgcgggcg gcttcgactt
     2581 cgtcaggatt gttgccttct gaagcttgaa ccaaaactga atatttgtcc ataggaaact
     2641 tagaatctac aggaagatat gtaaagtctt tgtttttccc gttaggaatt ttaattgcgt
     2701 attcaacatg gtcggtagag cgcggattag gtttgtaatt gcgcacaaac atgccaggaa
     2761 tggtttgttc aagcatacga ccaagctgag cttcgccaaa tgcgcctcga gttccaacat
     2821 ttgtcagcac cttgcttaaa ttatcaactt catcagatat tctttccatg tgaataatac
     2881 gctcaaaaac attatcaagc tgctttgaca cggcatccat cttgacattt tgctcgacag
     2941 cagccttggt taactggtca gtgccagttt taggcttttt gaaaaccaag attaaaattg
     3001 cgataattag cagggcaata attacgaaac acaaaatctc taccatttct tcctccattc
     3061 atcaaatttc attataacgc gaaaaagcaa actttcttta aaattttgta tataataatc
     3121 ggtgtcgagt aaaggagttc gatatgggtg ctcaatccat tttaacaaca agtattggtg
     3181 ttattgcttc acaaaacgcg actactggaa cttccgataa tctactcttt atagcaatac
     3241 caatcattgc cgcacttatt gctttagcgc tgtccccttt tgcttttaag cgaattcatt
     3301 caaaccacgg cgcaagcgta attgaggtcc gcaaaattac acttgtttct attgcttcgg
     3361 tccttattag tgcgttgtgc attcttttct ttttgagcaa tgcgcagcct gcaagcgccg
     3421 cgaatggaag catcagcatt aacgatcata ttgatttagt cttagatgaa aataacgaag
     3481 tcgcaagcgc agatgcaaaa ataaccaacg gaacaggtga aatatatagc ttggacacaa
     3541 ttcgtgtcat atcaaacaac gaaagcattg gtgatgatat atgaagcatt aagattgttg
     3601 agcaagatta tgaaatttac gatggaggcg ctgattctaa aaatgacatc gcaaatgttt
     3661 tgcagccgaa ccaaagcgca actgttcgta ttagcttgca gcttagtggt gacgaagaaa
     3721 atatccttag tggaaacatt gcctctcttg agattgcaac aaaaccaaca aaagttagtg
     3781 tttattacac aaataacgac gagaaaaagt ctaacaacat aaacttcaat tttgattttg
     3841 ccgaatttga gaatgcagat aacactgtgg tgaatagaaa gctggcaact gtggcttcgt
     3901 tattagatac taaaatgtat gaagctgagc aaattgttgt ggaaggccaa acctatgaca
     3961 aaaccaagaa aactgctctt gaaaaggcct taggttttga agataactat tactatagaa
     4021 tgtatgatgg tgaaacacaa tctgacccag gtgttgacat tgttaaacca gatctaagca
     4081 ccgagaaaaa tgatacaact tcatatgaat ttgcacataa gaaaatcttt tttggtgatc
     4141 actatagcga gattgtttcg gttaacattg ctggaactca atgtagcgaa gaatgagtaa
     4201 gcaatcttga ctttggggct gatacagata actattttga tgcaacagga aagcatccgc
     4261 tatgaataga taagcatatg cataagggtt ttgaaattgc ggcgaaccgt gttgaatacg
     4321 ccttgaatga ttatcttaat aaatacgttc aagatgacgc taacacaaac agaatacttt
     4381 ttattagcgg tcattcacga ggagctggcg tcgcaaatat tgtaggcaga gatttcgaat
     4441 acctggcaaa tgccaagaca tttacttata catttgctgc tccaaacaca actgatgaca
     4501 ttgattcgaa gcttaactat aaaacaattt ttaacatcat gaatatggat gacattgttt
     4561 gtcaaattcc ttgtacgaat atgggatatg acaaatacgg aagagatgtt tgcctgtcaa
     4621 tttcgactgg gaaatctata actggaggag atttaacaga cgcctttgaa tctattatga
     4681 tggatgacga atacggtgga aatacgccaa ccgaaatttt taacttgact gaattgttga
     4741 gaaataatat tgtcaaggga acaagagaac aatgctacga aattggtgaa gatcaaacta
     4801 tatgagacta ttgcgttcat gatgacgaaa tagatcctct ccccgaagca gaagcgaatg
     4861 caaaaattac tgaaattaaa caaaacctta cggaaaacgg tttgaattat tacagcatta
     4921 tagagaaaga acaaattgaa ggcgcggaca aatataggat cattagatga tataacccag
     4981 ctttcttact gaaagacatt tctaatataa ttcttggaaa caaacaactt cctgatgatt
     5041 tatatgatta cagcgaagct gtttgagaca ctattaatca ggttattcct cttgtttttg
     5101 gtgttgcgga cgggcatatt ccgatgtctt atgtggtgct gaccacaaac gcagtctact
     5161 aatttagagt ttctaaagtt ctaattagtg ttgcaaaatg atacggcaat gattttgtat
     5221 agaaagtttc atttgtagag tgttcgcctt tgacatcggc gccaaaagat atgcattgca
     5281 tgcttgggcg tttatcaagg aaaattccaa cttctaagcc gccatgcgtt ccatttacaa
     5341 taacgtcaat gtcgcaaact tctttcgatg ctcgtgtgta agcatttagc aatttgcgat
     5401 ctttggaagg atatcaagcc ggaagcgtct cgataacatc gtaatttatt tgtaattttt
     5461 tagaaacttc atcgttaaac ttctcaagct tttttaagaa ttcctcttcc ccgaacctat
     5521 acattaaagt aaagtcaaga gtgccatctt taatgttgac cactcccata tttaaagaat
     5581 tcttagggac taattcaacc tcttcgaaca tatcaaaaag ttcattgtgg aaggcgttga
     5641 gcaattcggt aatttgcttt gtggcttcgc tgttaaacac ctccgaagtt aagtcgctca
     5701 cttcaaaatt gcacttgcca tttttatcat caacgctatt tgccatttga tcgtctatta
     5761 cagaattaca aatttcttta attgcgtcaa catcattttt tgaacaagcc agcttcattt
     5821 ctgctgaata agctattgcg ttgaagtttt gaccgccttc gagactaatt aaatcgaagt
     5881 tgatgttgcg ctcaactaag cggttcatca cattacgaag aacatctatg gcgctaactc
     5941 gtttgtaaat tattgagtta gcagaatgac cgccgagcag tccatcaaca ttaattttta
     6001 tttgagtatc atgctcagat tttgtcattt catagttctt tttataaacg aaggtccgaa
     6061 caccacaaga aaaaattcga acaatgccag cttgacggcc atcaatgtta accatgaagt
     6121 ccgaatcaag aacttcgctg cttagaccat atgcaccatc cattgttgtt tcttctttgg
     6181 tggtaaatat aacacgaagc gggccgtgct taaaaccata gtcttttgaa attgcaagaa
     6241 gagtgattat accttcgcca ggatcggcgc ctaaagttgt ttcgaaattt cttgaatgaa
     6301 ttgcaccggt ttcttcgtca taaactgcat caactggttt tggatctggc gaagcggagt
     6361 tcttagtctc ccaaaccata tcctggtgag tttgaaagat tattttaggc caattttcac
     6421 aacccggact tgcaggaaca tcccaataaa catttaggga ttcatcaaca actggattta
     6481 gattatgcgt cttcgcccaa ttagcaagat gctcagaaat gtgagtgcaa tcagctgttg
     6541 ttcgaacaac tttgcataaa tcaaggaaat cggcaatggc agattcagca actttattag
     6601 ctgcttcctt gtagttttcg tttatcgtag cgttttgggc aacagtatta ggatattcgt
     6661 ctataacgac actgtttgcc ataattattt tgttttgacg ccttttttat tgttatatgc
     6721 ttttgcgatc aacggcaaaa ttagacccat cgctattaac acaataggaa ttgcgacgtt
     6781 cataataaac ttcgcagggt cgtcgttttg catcttcatt atcatgccag caaaagttat
     6841 gaccgcacac caaagaccaa caaatatacc aagaccttta ttcttgatga aaatgaagtc
     6901 atctttatgc ttcttaactt tcttcttatc tacctttaat gctatatatg cgacaaatac
     6961 gcaaatgtag aaaattggca taacaattga gcaaatctca agcaatcatt taattaggtc
     7021 gtccgccgta cccattgcaa gagatggaac aataagcaag aaaacagtaa ttacagttac
     7081 aacgagcaac caataaggat aagcaccata tttattcttc ttcaaagaac ccttagggat
     7141 aaatctttga tcactatctt caagcaataa acgaagcgga acatcaatgc taacaataag
     7201 aacagcgcag tctgtgatta acttaatcgc agcataaaga accatcaaaa cattacccaa
     7261 gccaaatgct tgaccaacct tttggaaaca aatatattgt ccattagtaa tgaagtttgg
     7321 atccataagc actgttgttg attcgccatt tgcaacaatt tgcattggag tataagtctt
     7381 gtttggatca aggttgtttg gatcagcaat catgccttgg caatcttgac cgaacattag
     7441 acccatagca attgcaccag cgaaggcagc aactgcaacc ataattacaa ctgcgataat
     7501 tcccttcggg aagtcccgtc caggcttttc cattctatta acataaggag caatcttttc
     7561 ggcgcctcca acaccaaaca gcaaaattga aagcgaagca aaaaccgaca tgtctgttgg
     7621 aacaaagctt gtaggatcaa gatttaagct gtaggttgga atttcgcttg ggttgacatt
     7681 gaaaattgga gctaaaaggc caaaaacaat gaacaacatt gccatgataa aactaccagt
     7741 accagctatt gaagcaattg ttttaacaac cttaatgcct cggcttgcca aaaatacgca
     7801 aaccaagaaa atcacaacac caattagctg aacagtcata ggatcaatgc ttgaaatatc
     7861 accattttgg ttaaccatcc agttaaatgc aacaataatt agacttggct tctgagcaag
     7921 atatggaagg attactaccc aaccaattca tcctgcaaaa taacccattt taggtccaat
     7981 tgtctcaagt gttcaggaac taactccacc accatgagat ctaaatacag aaccaagttc
     8041 tcctaccatc aatgaatatg gcaaaaaata cagcaagaat aaaacgaacc aaacactcat
     8101 aacacgagtt tgtccgaagt aaaaacatcc attgattaag ttgttaaacc cccataaagt
     8161 acaaaaagtc ataacagcaa tagaaggtca ggaaattgta gtttccattt ttggtttagc
     8221 cacttcttta gccatgattt ctccttaagt ctcgcttgtt gcggtattat actaaaaagc
     8281 ctgcttattt aacctaaaaa tagaaattta cgagattgta tgctgaatca agattgcgga
     8341 ggttactgag cctactccac caggtacagg ggttattgcg ccaacgattg gctcggcttc
     8401 gtcaaaatta acatctccac aaagtttttt tgcgatagga tctcaattga taccagcatc
     8461 gatgacaact tgcccttttg tgaaatactt tgcgccgaat tttttaggtg tgcccgttgc
     8521 aacaatgacg atatctgcat ctttcgtgat tttttccata ttttgagtct tggaatggca
     8581 taccgtgaca gtggcatttt cattttgaag catatttgca acaggtttgc ccacaacaag
     8641 gctacgacca atcacaacag ctcgcttgcc ttctacttcg atgccaaagt gatgcaaaat
     8701 tgccatgcaa ctttccgccg tgcacggagc aaaagcattg tttcctaaat aaggaccagc
     8761 aacagaaagc tgtcttgcgg catctatgtc tttagaagcc gaaattgctt cgcaaatttc
     8821 attttcacac tgctttagct cccctggaag aggcctcatg agcaaaatgc cttgaacctt
     8881 gtcatcttcg tttagatctt caatcaaatc aagcaagtct tgtttgtcaa tagtgctctc
     8941 gcatttgtga acatcgaata aaacaccagc ttcaccagcc tttttttcga tgccttttaa
     9001 gtaggaagcg cttgagccgt cttcgcccac acaaacaaca tcaagttttg gaacaaagcc
     9061 atccgctgtg agcatgtcgc taactttaga tgcgcattca gaaataattt tcgaagcaac
     9121 ttcttttccg tctaaaattt tggccattta aaacaacccc gaaattacgc cgtcttcgct
     9181 gacgtcaatt ttttcagcgg caggaacttt tggaaggcca ggcatagtca tgatctcccc
     9241 agtaagggcg acgatgaacc cagaaccagc tgcaagctta agatttctaa ctgtaatttt
     9301 gaaatctttc ggaactccgc gcttgttagg atcatccgaa aaactgtatt gagtttttgc
     9361 aacacaaact ggaagctttc caaaaccttg cttttctaaa acaggaagtt gcttaacagc
     9421 gtttccaata aactcaactc catcagcatg ataaatcttt gtagccaaat cattgagttt
     9481 ctcagcgata cccttatcca agtcatagct aaatgcaaac ttagagctgt cttgctcaca
     9541 caaatcaatt gcggcctggg ccaggtcttc ggaaccttta gatccattca tgcaagtgtg
     9601 cgaaacttga acgttaacat tagcatcctt gcaaagtttg tagagaagtt caatttcctt
     9661 atcagtatct gttgggaaga cgttgataac agccaagcat ggaagcttgt agacgtcttc
     9721 gatgttctta acgtgctgga gcaagttcgg gaagccagct tcgagagcct tgaggtcttc
     9781 tttcttaagc tcctctttca tctcggcagc cttcatgcct gcagctgcac caccatgaag
     9841 tttcaacgca cgaacggtcg caaccataac aacagctgac gggcgaattc cgctcattct
     9901 acatttaatg tcgaggaatt tctccgcacc aaggtcagca ccgaatccag cttcggtaac
     9961 aacatagtct gcaagttgag ttgcggtctt ggttgcaatt accgaattgc atccgtgggc
    10021 gatatttgca aaaggaccac catgaacaaa tgctggagtg tgctcgagcg tttgaacaag
    10081 gttaggttta atagcatctt tcaataacgc ggtcattgcg ccttgagctt tcaaatcaga
    10141 agctgtaaca ggcttttcgt cataagtata accaacaaca atgttgccca agcgcttctt
    10201 taggtcattt aagtcagttg ccaagcaaaa gacagccatg atttcgcttg caacagtaat
    10261 gtcgaagccg tcttcgcgag gaacaccttg catctttcca ccaaggccat ccacaatgtt
    10321 acgaagctgg cggtcattca tgtcaaccgc gcggcgccaa gtgatgctct tcggatcaat
    10381 gccaagatca tttccctgtt gaatgtgatt gtccaacatt gcagcaagca aattgtttgc
    10441 agcaccaatt gcgtggaaat cgccagtaaa gtgaaggttg atatcttcca taggaactac
    10501 ctggctatat ccacctccgg cagctccacc cttaattcca aaaacaggac caagcgaagg
    10561 ctcacgaaga acaagcatca cgtttttatc gctgtctttc aaataagcgc gcatagaatc
    10621 agccaaacca acagaaatgg ttgtcttacc ttcgccagca ggcgttggag ttagagcggt
    10681 aaccaaaatt acctttccat ttttgttagc cttaactttg tgaatgttga ccttcgcttt
    10741 atagttgccg taaagctcga gcgaatcttc ggcgattccg gcttccttcg caacttcgcg
    10801 aatgtcattc attggggttt cttgagcgat ttcaatatca gatttcattt ttgtcctttc
    10861 ctagatgctt tcacctggta atattgggtg ccgaagcaat ttcgttagct cttcgcgcgt
    10921 ctttcgcatt gcggtgattc tgtaatcttc gaaattctca atttcatttc tgccttcaca
    10981 gaatcgcgaa ctatcttcta aattaacttt gcttgacaca tcaacaagcg caattttact
    11041 ataagtttga ccgtcgcgcg cgacagtttc aacatttaac aaacccaatt cagcgaaaat
    11101 atcaatagcc gtaagcatgg ttgccgccga agccacaacc gcagcttgct cgaacaattt
    11161 ttgatgagct tcgtggcaaa attctcaagt gccgttgtta tttttgacgt tttcgcgaat
    11221 tgccgcataa attccacgaa ggttttcttc cgttgggtta gtcatttcca aaatttgggt
    11281 gttgattgtg gcatccgccc tattgtagag aaggtgcaca attgcgggat tgttgtcacg
    11341 accgcatcgc ccagccattt ggttgaactc aacattacta aatggcaaat gatacaaaca
    11401 aacatggcga atgtcgggga tgttgacgcc ttcgccaaac gccgaagttg caaccaacac
    11461 gcttagctca ttttcgcgga ataaagtctc aattttgttg cggtcttccc tgctcatgcc
    11521 tgcattataa aatccgactt tcatagcaat atgaggcgcg cgggatttta taagacgcgc
    11581 aaggtcaacc gtttgctgtc gcgaattaac ataaattaca attttttcac cactggcgca
    11641 aagcgcaacc aaataatcat ccctactttt aatgttacgc ctgtcatcaa caaacaaatt
    11701 ctcacgctca aaattatctt taacaacttc ttcaattggc aaaaactttt taatctcatt
    11761 gcaaatgtca tcattaacgg tcgcgctcaa agccaacact tgcggatggt ttagcttctc
    11821 aataatttta ggcatatgtc cataagcggt gcgctcgcca atttttactt gccccatgtg
    11881 gtgagattcg tcaataacaa taaaaccaat ttttgcatgt tgcgcaattt tgtctatgtg
    11941 aattgccaaa aattccggag tggtcaacat aatgtcaagc ttgccctcgc tcataagttt
    12001 gtaattttct tcgcgttcat cggcaggagt ctcaccattc ataacacgaa tattcaatcc
    12061 aaaatcttta aggttttttt gcaaatggaa agcttgatca gaaattaaag cgcgaagggg
    12121 gtagacaaaa atgctcatct tcttattgca aacagcttga gcagcggcga agacctggaa
    12181 aatcaagctt ttacctcggc ctgttggcat aacggataaa accgatttcc ctttagcaag
    12241 taaatccagc gtctcttgtt gagattggtg cagtttccca tccttgccaa tcatagcttc
    12301 aacaatcagt tgctggactt cgtcgatttt tttatctcta aaatcttggg cttcattcaa
    12361 attttcagcg ctttgatttt gggcagagcg ctgcatcatg tcgcttgaaa gttcacggac
    12421 agaatcctta atgaaatctc caacgcttac aagatttttt tccttcacct caaacttatc
    12481 aaaaagttga ctaacgaagc tgccacccgc atttgtgtct tcggtattgt gcagctcatc
    12541 ggtcgggata atgtggtcaa tgtgcatctt gacgtttttg cggccttgga actcctcaac
    12601 cttcggcgtg taaatgacgc ttacggtgct gtctaaggcc aggtacttat caatatttga
    12661 aacattaaac ataattccca tgccgccata cgcgccatcg gtgatattgc aagataagtg
    12721 gttatggttt tggccgacag ccttcgcgcg cctaataaac acattagagg ttatgaactt
    12781 cggctcttcg ttggcattac caaaaggctc catcacgttc aaactttcaa tgtcttccac
    12841 attaagctca tgcaaacgaa gctccagatc cgacttcaag actttttgaa attcagagat
    12901 gtccaaactt tgcatgtagc cttcaagctt ctcactaaat tcagcaacgt cttccgcttt
    12961 tagagtagct ccacaagcac ctttgtgacc tccaaactta atgaacttat caaaactttt
    13021 gctacaagct tcgtgcaagt caacgttgcc aacgcttcga ccacttccat gagcaacgcc
    13081 atcttgcacc gacatcacca aagttggaac gttataagat tcggcaagct tagctgacac
    13141 aattccaagc actcccgggt gccaagcttc gtcgcttaaa actaaacttc gcttgccggg
    13201 gttatcctca atgatttttt gagcgcggaa ttctgcctgt tcaagcattt tctcagtaat
    13261 ttcacgacgc tggttattaa acgcctcaat ctcaattgca atgttgtagg cttgagcgta
    13321 atcatcacac agcagcaagt ccactgcctt ttcaggctca tccatcctgc ccgctgcatt
    13381 taatcgaggc gtcaaaacat atgaaacatc cttcgccgtc atgcttccag cttttcttcc
    13441 agcaacatct aaaatagcac gaagagaagg cctcggctgc ttgttgatca tctcaagacc
    13501 ttgagttacc aaagctctgt tctgaccaat gagcggcatc acatctgcaa ttgttccaag
    13561 agtggccagg tcaataaaat ccatccacaa atgcggaagc ccaagcctgc ctcccaaact
    13621 ttgaataagt ttcagcgcaa caccaacgcc tgccaatatt ttatcgggac tgtccccgtc
    13681 ttcggcttct gggtcagcca ttgcaatgcc aacgggaatg tcgccagact tttcgtggtg
    13741 gtcggttaca acaacatcaa ttccgtgcga ctttaaagtc tcaatataag gcgcggatgt
    13801 tattccgcaa tcaaccgtaa taattgtttg tggtaagggg tccatctcaa gcagcctgtt
    13861 aacgctgtct tcgcttaaac tatagccctc agaaaaacgc ttcggcaaaa aataatccac
    13921 tgtcatacca gcgtttatat gcttatcaat agccgacagc ccccgcatca tcacaacagt
    13981 tgcactaatt ccatccaagt cataatcacc ataaatcaaa attctgtggc ccgcacgaac
    14041 agcgttctcg acagcatcgg caacttgctc catgcctttt atgtcatagg gattccccca
    14101 atctcgttca agactgggct tcaaaaactt ttcaatttca gtttggtcag tgatgcctcg
    14161 aacttcaagc atcctcgcca acaacggcga aatctccata ctgctcacct tacttaatac
    14221 tcgctaacgt ttcttataac taaatttatt ctatcacaag atttaattat tattaaatct
    14281 attgcgggtg aaaacatatg caccaataac agcaatagac aaaacagcta atgcaaataa
    14341 agctgataca tcgccagttt gaggtgttgg agtaacaatt tctggatctg gtgttgggct
    14401 tggctctggc gaaaatggag ttaggcttga atagaaaaaa tcagttacgt tttcgccttc
    14461 gttgtcaaga agtgtaattt ttgaatatgc atgatatgga atgtgtgcaa tctctgttga
    14521 aggcatcgcg taaaatgtgt atccattagt ttttagaatt ggaagaccgc ggctatcaag
    14581 gccaatgttt tcaacagact tagcaatgtc attttgtaaa tttaagttaa gataagttct
    14641 actatcattt tgagcagcca ttacaaacgg cccgtacatc aaacttccaa catagcttcc
    14701 atcaagttta tctggcaaaa gttcaacgtg gtatgtccaa ggcatattta caacaatggt
    14761 gtctccagca gaaacactaa cacttacata cgacgaaggc ttggcttcgg cgcttacagc
    14821 aacaccattt ttggtaacac taaaaccact cgttgcccat gcaggaactc gaagtttcat
    14881 aacaaagcta ccgcttcctc ccactttgat tgttgaagtc tgcgaaggga aagtagtttc
    14941 ttgagt
//
LOCUS       DBNCDC010000015        14873 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_1341_length_14873_cov_7.944999, whole genome shotgun sequence.
ACCESSION   DBNCDC010000015 DBNCDC010000000
VERSION     DBNCDC010000015.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 14873)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14873)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14873
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1341_length_14873_cov_7.944999"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            1..444
                     /locus_tag="AC04VQ_02085"
                     /pseudo
     CDS             1..444
                     /locus_tag="AC04VQ_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            469..1038
                     /locus_tag="AC04VQ_02090"
     CDS             469..1038
                     /locus_tag="AC04VQ_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553016.1"
                     /translation="MMDFNIDGNDIKVGFSVCRDAQFYPEVYRYYAASNVDLLVHFSA
                     SSLTRFARECVLGSYSSRDKIGVIACNLWGADGPPENVSTLKKNSLICSPRLAGTDEY
                     DNDGRDIELYGFGLSEMSPEGLEVGTTLQTGRGMDLFNCGFSKGRLNFEVVAKMFDEL
                     SSKYYSDYKSLYPGSRSRDPLSKPIKISY"
     gene            complement(1035..2231)
                     /locus_tag="AC04VQ_02095"
     CDS             complement(1035..2231)
                     /locus_tag="AC04VQ_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIO9553017.1"
                     /translation="MVFFFAITITGRIVVAPSPFLPIDELCTLLDYADAEFICHDHSI
                     DTGAIKKEMSTEIKYIDIDKSENIIKKLDSAELKSVNLKFCTNPQDDAAILFTSGTTG
                     RSKGVVLTQQNLANNASATDFEYEHHKVGLSILPFHHVLPLVTTILIPVALGMCNCLS
                     KSINTIFEDIIRFQPYYTSIVPSMLKPISKMLANDSSILKDNFSLKAVACGGAPVDEK
                     YQILFEKIGGTLLSGYGMTETSSMVSNNYRYTKKFGSYGNVINNCEVRIVDGEIQVKG
                     TNVMKEYYKMPEETKNAFDGEWLKTGDIGYLDDEGFLYVTGRKKNLIITSNGENVSPE
                     QIESKLCSFGPIQKAKAFQKEDKIMVDLVLEKEANEETAKELIKEYNSTQPNFRKIAK
                     FNIVSN"
     gene            complement(2232..2444)
                     /locus_tag="AC04VQ_02100"
     CDS             complement(2232..2444)
                     /locus_tag="AC04VQ_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553018.1"
                     /translation="MKKYSTLFDLIHETSKNNLNRPAIKFVSNNEEVTITYEKFYEDI
                     LLVAKYITSISEEKSHFAILSQNSYE"
     gene            complement(2444..2668)
                     /locus_tag="AC04VQ_02105"
     CDS             complement(2444..2668)
                     /locus_tag="AC04VQ_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIO9553019.1"
                     /translation="MLEKIKDCISKVSDIDISKISGEDRIDSLGLDSFEIVCLVSEIS
                     DCLNLNLEIGDMSEIETINHLIKFLEKKSS"
     gene            complement(2652..3005)
                     /locus_tag="AC04VQ_02110"
     CDS             complement(2652..3005)
                     /locus_tag="AC04VQ_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553020.1"
                     /translation="MHNNKYDVTCEVEIYAVVDFKSFYDIGYGTLRQNKDKIILKLKD
                     GTKIFEHHHKECYSLYADFYFYGYKDAISFGNSRIQFYCFTELEDYPVLKARFAAEEA
                     YKMLKSKESKNVRKD"
     gene            complement(3039..3476)
                     /locus_tag="AC04VQ_02115"
     CDS             complement(3039..3476)
                     /locus_tag="AC04VQ_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553021.1"
                     /translation="MLKYFKVPVINIKTYGAYQRRPQYNNAHNTKETRNVNVHAEMEY
                     LFSKEDLKKLSGDQINKKLKEVFTYDHFRWQFENKIKVDETFRAKGLHHALYKCPNCG
                     SEGKTTSEGSKLKCTKCNKEYELTEYGKLKALNGKTEFEYITD"
     gene            complement(3522..3962)
                     /locus_tag="AC04VQ_02120"
     CDS             complement(3522..3962)
                     /locus_tag="AC04VQ_02120"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIO9553022.1"
                     /translation="MFTTMKVTKKSYEDVINLPIPKHEKPVRGLKYFRKFAYKTFLNE
                     LNKNNFTYKKIGMEKLKKNEPAIYIMNHSCSVDLEILVKLIGNRHFYIVGTHDAFIGK
                     KTLMKYCGGIIPAKKLVPDLNLITDISHVLNNLKCSIFLLPEAS"
     gene            4088..4852
                     /locus_tag="AC04VQ_02125"
     CDS             4088..4852
                     /locus_tag="AC04VQ_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553023.1"
                     /translation="MKDAKTMAFANAYGVLAALENLCEIDEEAKEICSKIKKPVALCF
                     DVKNGPCITLEFSTQGCKFVEGDKTCTCKMSFGSPEKFNNLIDNSKPGRPSKNPAQVL
                     KFLLGPFTKLTNILEKYLRASKEDLKNKVFFERSTILTMYVIGGAICAVGNNDKISKL
                     SASAIVDGDILMGIKDKVHVTVHCKNHHLTLTKDTCKKPRAIMEFNSIELANKLFNGE
                     ASAIAELCAGNIYMSGMMNMVDNINRILDRVAVYLA"
     gene            4869..5984
                     /locus_tag="AC04VQ_02130"
     CDS             4869..5984
                     /locus_tag="AC04VQ_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIO9553024.1"
                     /translation="MAEKFPEYKHEKSKKWFDRALAAVPSGVPGHLGPSEGLFLPITK
                     WPLMCDHAKGTYFWDVDGNKYIDYMCAYGPNVLGYCDPDVDKAAIEQLKKGNCTTSVS
                     YKMVECAELLKDTVYTADWSFFMKNGGDATTFATICARAHTGKKKLIFFKGYYHGVAQ
                     WTQKADYPGILEEDVANNIVVPWFDIEALDKAYAESKGDIAAIIAQPYDHGNFYDNVC
                     ASKEQWHKVRVWCDKHDVLLIIDDVRCGFRLDLAGSDHYYGFKADLICFCKALANGYN
                     MSCVCGPEHMKATASSVTFTGSYWLSAEPFAACIACINKMKKLDTPTLFRKLGTQLTD
                     GLVKAAKKHGFKLVVSGEPALFYLRIANDDNLMLHQE"
     gene            5994..6137
                     /locus_tag="AC04VQ_02135"
     CDS             5994..6137
                     /locus_tag="AC04VQ_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553025.1"
                     /translation="MVSRGIFLASHHNHFINAALTEKDIKHTLEVAEECFKIVAKRHP
                     EIK"
     gene            6270..7049
                     /locus_tag="AC04VQ_02140"
     CDS             6270..7049
                     /locus_tag="AC04VQ_02140"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIO9553026.1"
                     /translation="MGGALSSMDVLTILYHRFMSLDKNNPEWEDRDRFVLSKGHGAVG
                     YAPVIVDYGFMPIEELQIFNLTGSKIGMHLDSNKVKGIDFSTGSLGHGISLGVGAALA
                     AKMKGKDYMTYVLCGDGEMNEGSNWEGIMSAAQYKLSNLVVFVDNNKCMIDGRVEDVM
                     NIEPIDKKFEAFNFNCVRVDGHNFKEMDTAIEAAIKNAKTGERPTAIILDTFKGEGVD
                     YMRDNYLWHYGALDEQKAADAFASLDKYYQERVARVEEEGK"
     gene            7052..8065
                     /locus_tag="AC04VQ_02145"
     CDS             7052..8065
                     /locus_tag="AC04VQ_02145"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIO9553027.1"
                     /translation="MAGMVYTSIDAEKLTTAEFYGIALRDLASEHDNVVALTADLAKS
                     TKIAAFAKEYPERLINVGIAEQNMFGVAAGMAKNGFVPYTSTFSIFACARSLDQVHTD
                     ICYQNVPVKILASHAGTSFGQAGSTHHAIIDLAVARSLPHMTVMCPCDGAEAYKLVLA
                     SYDIPGPVYIRINRGFDAVVWDGGPEAVDFQWDKAATMMDGDDITFIGIGAGVYQAIQ
                     AARMLKHDAGVNARVLNMHTLKPIDKEAIQKAVDETGKIVTIEDHVIQGGLGSAVAEV
                     VAEYGKPCTLKMLGHDNDFSTIGLQEDLLTIAGIDSNSIEKAAAKLLGISLKTVDNWN
                     DEY"
     gene            8079..8879
                     /locus_tag="AC04VQ_02150"
     CDS             8079..8879
                     /locus_tag="AC04VQ_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553028.1"
                     /translation="MATSEETKYANKVFMAGALPLMKIIANEDPKLSATFKRKKLNAV
                     YQVSAMDEGQKHAVHFVVENGVWDVFQGEYNGPKKIDAELAFASMEKMNGFMKSDMKQ
                     LPSFKIKSVTKFAQFMKVLLKMSSLLTADKPPKDVETQRFLTKLYFCLLSTGISALNK
                     MGHPEISKWVAESPDRTYQWTIVGDPDTSAYLRICEGNSKAGKGLYERSDPFFTMAFD
                     CPESALLILMEIGDMFDMTANRQLIMKGGPEFGVQLGDHMTTVGHLAK"
     gene            8888..9175
                     /locus_tag="AC04VQ_02155"
     CDS             8888..9175
                     /locus_tag="AC04VQ_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553029.1"
                     /translation="MNKTQIAKICEAQRAFFATGKTLDVNFRIVQLKRLREFIKRYEN
                     QLEAAMKKDLCKSEVEGFLCDVGPSIMEITETIKGLKKWARSETHFSGLLC"
     gene            9182..10033
                     /locus_tag="AC04VQ_02160"
     CDS             9182..10033
                     /locus_tag="AC04VQ_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIO9553030.1"
                     /translation="MVTKVHKMPYGNCLIISPFNFPVLLTFGVLAACISGGNTAVLKC
                     SSKSSESTKVMKKLIRDAFPQKYITVIDGGHDVADMCLDQRFDKIFYTGSPKVGVHVM
                     QKAAKNLTPVALELGGETGNWCVIRKDANLKDAARKIAFFKACNSGQICININQIAVA
                     REVAEPFIEELKKAFEKQLGTNAIQNDEYPKLITKAAYDKCDNWVKKYKSKLVYGGQG
                     DPKTHKYNPTILYPMSADDDICKNELFNPILPIVPFADNQIDKVLKMIASREHGLALY
                     LFTSDIA"
     gene            complement(10429..11250)
                     /locus_tag="AC04VQ_02165"
     CDS             complement(10429..11250)
                     /locus_tag="AC04VQ_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553031.1"
                     /translation="MATKSTKTKAKSTAKKTTAAAKKTTAAAKKKASASVKKATTAVK
                     NVKFTDVLNSVGAFFKNLDKAYYVAFLAILGIILFFMPWLNITVLTKLYESLHIDFLS
                     GLFAIPQTLLSIFFSVDAQINGLNAIVDKFATINTNLDSLHDVTQYINTICSVLGIQV
                     AANGETVNGIIAAIQNALPTADQLAAAKQSLASVGSIVKAGSAAVTLWVLVVVGFNAY
                     AAIICIAKKKITKGCTAAFIIEGIFVFLFMLICLAVSSVVQSLVFIIPNIFEATG"
     gene            complement(11258..11803)
                     /locus_tag="AC04VQ_02170"
     CDS             complement(11258..11803)
                     /locus_tag="AC04VQ_02170"
                     /inference="COORDINATES: protein motif:HMM:NF015674.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009268 - response to pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HIO9553032.1"
                     /translation="MAKQIGKMSKGWWLYLILGILATLFGIALFGNMAFAITTVAVIA
                     GIYFLFSGIFGAVITIVDRQYIHLWGLKLALHILVIIAGVCMLTRPAFALSFLWFICG
                     FGFLFDGISMIILSLEIKKANVGSWVLLLILGILVALAALSIMGNPILGLAIVAISAA
                     ISAIVFGVSNIVYAFQIKKLG"
     gene            complement(12018..12503)
                     /locus_tag="AC04VQ_02175"
     CDS             complement(12018..12503)
                     /locus_tag="AC04VQ_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553033.1"
                     /translation="MTLYDYYNYVRQFKKTKADGFENMNLGEAFFEPMKEVVKYLEDY
                     GFIVYICSGTDRFLDRALLSDIFPEIPSSQIIGMDYKVITKNQGSTDPLEYEYQPGEN
                     LIRTSELIQKSIKMNKAAIMAQEIGKLPVLSFGNSTGDASMLNFTISNPDYKSLAFII
                     K"
     gene            complement(12537..12665)
                     /locus_tag="AC04VQ_02180"
     CDS             complement(12537..12665)
                     /locus_tag="AC04VQ_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553034.1"
                     /translation="MLAHRILDDPDYKDKATPQEIELAKGIRSLKQDSKIPSNYEK"
     gene            complement(12825..12947)
                     /locus_tag="AC04VQ_02185"
     CDS             complement(12825..12947)
                     /locus_tag="AC04VQ_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553035.1"
                     /translation="MSKSKKMNKSVKTGLLITLCVIVVVGVLAFIFCRPSYFKS"
     gene            13096..13542
                     /locus_tag="AC04VQ_02190"
     CDS             13096..13542
                     /locus_tag="AC04VQ_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553036.1"
                     /translation="MNKTNFANNFIIKVVLGIIFAVLIFLSVLALGCSFDKSADLVVY
                     GNIYTAEDDNDSMAEAFAVKDGKYIYVGDKNGVKDYIKEGKTEVIDRSSEGMVMPGAT
                     EGHSHYFGQSAIETCLPVSNCSFNEMIDALKAQVANVKPEKFFAFG"
     gene            13627..14873
                     /locus_tag="AC04VQ_02195"
                     /pseudo
     CDS             13627..14873
                     /locus_tag="AC04VQ_02195"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
BASE COUNT         4580 a         2471 c         2924 g         4898 t
ORIGIN      
        1 gtaaatactt gttgtccagt ttggggcaat aaaaaatctg gtcttgagaa tatggaaaaa
       61 tatattaagg aagctggcga aaaaggaaac gttgatattt tagtttttcc tgaagttgcg
      121 ctaactggtt atacatacag cgaaggtaat tttaactgag ttgattacaa tcctttaact
      181 aacgaatcta aagcaagtga tggaaagacc gaagctcatg ttgctttggc cgaaacaatt
      241 cctggtcctt ctacagaata tttatctgaa tatgcaaaac aatacaatat gtatattatt
      301 tttggaatgt gcgaagttcc tgaaagtggt cccgttaatg ataatggtat agataagtgt
      361 tataactctg ctgcaattct ttgccctgat ggaacaattg aaaaatatag aaaaattcaa
      421 ttaacgagta atgaaccaaa gtgaagcgtt tgttctgatg aaccaaaaat gatggatttt
      481 aatatcgacg gaaatgatat taaagttggt ttttcagttt gcagagatgc tcaattttat
      541 cctgaggtat ataggtatta tgctgcaagc aacgtagact tacttgttca tttttcagct
      601 tcgtcactta cgagatttgc aagagagtgc gttttagggt cttattcatc gcgtgataaa
      661 attggagtta tagcttgtaa tctttggggt gcagatggtc ctcctgaaaa cgtctctact
      721 ttaaaaaaga attctttaat ttgttcacca aggcttgctg gaactgatga atatgataac
      781 gatggtaggg acattgagct ttatggtttt ggattatctg aaatgagtcc cgaaggttta
      841 gaggttggaa caacattgca aactggacgc ggaatggatt tatttaattg tggatttagc
      901 aagggacgtt taaattttga agttgttgcc aagatgtttg atgagctttc aagcaagtat
      961 tattcggatt ataaatcttt atatcctgga tcacgaagta gagacccgct atcaaaacct
     1021 attaaaatta gttactaatt tgaaacaata ttaaacttcg ctatttttct aaaatttggt
     1081 tgagttgaat tatattcctt aataagttct ttagcagttt cttcgtttgc ttctttttca
     1141 agaactaaat caaccataat cttatcttct ttttggaagg ctttagcttt ttgaatcggg
     1201 ccgaagctac atagttttga ttcgatttgt tcaggagata cattttctcc atttgaagta
     1261 attattagat tcttctttcg ccccgtgaca tataaaaatc cctcatcatc aaggtatcca
     1321 atgtcaccag tttttaacca ttctccgtca aaagcgtttt tggtttcctc aggcatttta
     1381 taatattctt tcattacgtt tgtgccctta acttgaattt cgccatcaac aattctaact
     1441 tcacaattgt taatcacatt tccataggat ccaaattttt tggtatagcg ataattgttt
     1501 gaaaccatgg agcttgtttc tgtcatgcca tatcctgata gaagagtgcc tccaattttt
     1561 tcaaataata tttgatattt ttcatctacc ggagctccac cacatgcaac agcttttagc
     1621 gaaaaattat cttttaagat ggaactgtca tttgcaagca tttttgaaat tggctttaac
     1681 atcgaaggca caatcgatgt gtaatatggc tggaatctta ttatgtcttc aaaaattgtg
     1741 ttaatagact tacttaaaca attgcacatt cccaacgcaa caggaatcaa gatagttgtt
     1801 actaaaggca agacatgatg aaacggtaaa atagatagac caactttgtg atgctcatac
     1861 tcaaagtctg ttgctgaagc attatttgca aggttttgtt gagttaaaac aacgcctttg
     1921 cttcgtcctg tagtgccaga agtaaacaat attgcagcat catcttgagg atttgtgcaa
     1981 aacttcaaat tgacactttt tagttcggcg ctgtcaagtt tctttataat attttcgctt
     2041 ttgtctatat ctatgtattt aatttctgtt gacatttctt ttttgatagc gccagtatca
     2101 attgaatgat catggcatat gaattctgcg tcagcataat ctagaagcgt gcaaagttca
     2161 tcaataggca aaaacggcga aggcgcaaca acaattcgtc cggttattgt aattgcaaaa
     2221 aagaaaacaa ttcattcata tgaattttga gaaagaattg caaagtggct tttttcttcg
     2281 cttatagaag tgatgtattt tgcgacaaga agaatatctt cgtaaaattt ttcatatgta
     2341 attgtgacct cttcattgtt tgaaacaaat ttaatggcag gtctgtttaa attgtttttt
     2401 gaagtttcgt gaataaggtc aaacagtgtc gagtattttt tcattacgaa ctcttttttt
     2461 caagaaactt aataagatga ttaattgttt caatttctga catgtctcca atttcaagat
     2521 ttaaattaag acaatcagaa atttcactaa caagacaaac tatctcaaac gaatcaagac
     2581 ccaaactgtc tattctgtct tcacctgata tttttgaaat atcaatgtcg gatacttttg
     2641 atatgcaatc tttaatcttt tctaacattt ttgctctctt tagattttaa cattttatag
     2701 gcttcttcag cggcaaatct agcctttaag acagggtaat cttcaagttc tgtgaaacaa
     2761 taaaattgaa ttcttgaatt tccaaagcta attgcgtctt tatagccgta aaaataaaag
     2821 tcagcataca aactgtagca ttctttatga tgatgttcaa aaattttagt tccgtctttt
     2881 aacttcaaga ttattttgtc tttgttttga cgaagtgttc cataaccgat gtcataaaaa
     2941 cttttaaaat caacaacggc atatatttca acttcgcatg taacatcata tttattgttg
     3001 tgcaaatcct ttttaacaca ttcgttttct cagtcatatc agtcggtaat atactcaaat
     3061 tcagtttttc catttagtgc tttaagtttt ccatattcag ttaattcata ttccttattg
     3121 catttagtgc actttaattt agatccttcg ctagtagttt ttccttcgct accgcagttt
     3181 ggacatttgt aaagagcatg atgcaatccc tttgctcgaa aagtttcgtc aactttaatt
     3241 ttgttttcaa attgccatct aaaatgatcg taagtaaaga cttccttaag ctttttatta
     3301 atttggtccc cggaaagctt ttttaagtct tctttagaaa ataagtattc catttcggca
     3361 tgaacgttga cgtttcttgt ttcttttgtg ttgtgagcat tgttatattg aggtcttctt
     3421 tgatatgcgc catatgtttt tatgtttata actggaactt taaaatactt aagcattttt
     3481 cctaagctct ccggaatgat tgtttctttt ccgccgaggc atcaactggc ctcgggcaat
     3541 aaaaagatcg aacattttaa attatttagg acatgagaaa tgtctgttat taaattaagg
     3601 tccggaacta attttttggc tggaatgatc ccgccacaat atttcattaa tgtttttttg
     3661 ccaataaaag catcgtgcgt accaacaata taaaaatgtc tatttccaat aagtttaact
     3721 aaaatttcta aatcaaccga gcaagaatgg ttcattatgt aaattgcagg ctcgttcttc
     3781 tttagttttt ccatgccaat ttttttataa gtaaaattat tcttattaag ttcattaaga
     3841 aatgttttat aagcaaactt gcgaaaatat tttagtcctc taactggttt ttcgtgctta
     3901 ggaattggaa gatttatcac gtcttcgtag gatttcttcg taactttcat agttgtaaac
     3961 ataataaatc tacccctttt tgattaatac gatgggttat tttatcatta taaaacacca
     4021 atctttttat ttattttttg tggtattata atcacatcta atttgtcaag cgaaaaggag
     4081 gttcatattg aaggacgcga aaactatggc gtttgctaat gcctatggtg ttttggctgc
     4141 acttgaaaat ttatgcgaaa ttgacgaaga agccaaagag atctgttcta agatcaaaaa
     4201 gccggtcgcg ctatgtttcg atgtcaaaaa tggaccttgc attacacttg aattttcgac
     4261 gcaaggttgt aagtttgttg aaggggacaa gacatgcaca tgcaagatgt cttttggatc
     4321 acccgaaaaa ttcaataact taattgacaa ttcaaaacca ggcaggcctt cgaaaaatcc
     4381 tgctcaagtt ttgaaattct tacttggtcc tttcactaag cttactaata tcttagagaa
     4441 gtatttaagg gcatcaaagg aagacttgaa gaataaagta tttttcgaac gctcaacaat
     4501 tcttacaatg tatgttattg gtggggcaat ttgtgctgtt ggtaacaatg ataaaatttc
     4561 gaagctatct gcatctgcta ttgttgatgg agatattcta atgggaatta aagataaggt
     4621 tcatgttacc gttcattgca aaaatcatca tttaacactt actaaggata cttgcaaaaa
     4681 gccaagagca attatggaat ttaattctat cgagcttgct aataaattgt tcaatggcga
     4741 agcttctgca attgctgagc tttgtgctgg caacatttat atgagcggca tgatgaatat
     4801 ggtagacaac attaaccgaa ttcttgatag agttgcggtt tacttagctt aaggagggag
     4861 gaactaaaat ggcagaaaaa tttccagaat ataaacatga aaaatcaaaa aaatggtttg
     4921 atagagcact tgcagctgtt ccatctggtg ttcctggtca cttagggcct tctgaaggat
     4981 tgtttttacc aattacaaaa tggcctctta tgtgtgatca tgccaaagga acttactttt
     5041 gggatgtaga tggtaacaaa tacattgatt atatgtgtgc ttacggtcct aatgttttgg
     5101 gctattgcga tccagatgtt gataaagcag ctatcgaaca attgaagaag ggtaactgca
     5161 caacaagcgt ttcttataag atggttgagt gcgctgaact tcttaaagac actgtatata
     5221 cagctgattg gtctttcttt atgaaaaatg gaggcgatgc aacaacattt gcaacaattt
     5281 gtgcacgtgc tcatacagga aagaaaaaat taattttctt taagggctat tatcatggtg
     5341 ttgctcagtg gactcaaaag gctgattatc ctggaatctt agaagaggat gttgctaaca
     5401 atattgttgt tccttggttt gatatcgagg ctcttgataa agcttatgca gagagcaagg
     5461 gggacattgc ggcaattatt gctcagcctt atgatcatgg aaacttctat gataatgttt
     5521 gtgcttcaaa agaacagtgg cataaggttc gtgtttggtg tgacaagcat gatgttttgc
     5581 ttatcattga tgatgttcgt tgtggcttcc gtcttgattt agcgggttct gatcactatt
     5641 atggatttaa ggcagacttg atttgctttt gcaaggcttt ggccaatggc tataatatgt
     5701 cttgcgtttg tggtccagaa cacatgaaag ctactgcttc gtctgttaca tttacgggtt
     5761 cttattggtt atcagctgaa ccttttgcag cttgcattgc ttgtattaac aagatgaaga
     5821 agcttgatac tccaactttg tttagaaagc ttggaactca gctaactgat ggtcttgtta
     5881 aggctgctaa aaaacatggc tttaaattgg ttgtttctgg tgaaccagca ttgttttatc
     5941 ttcgtattgc aaatgatgac aaccttatgc ttcatcagga atgaattgcg gagatggtaa
     6001 gtcgaggaat tttcttggct tcacatcaca atcactttat taatgcggcg cttaccgaaa
     6061 aggatattaa gcatactctt gaagttgccg aagagtgctt caagattgtt gctaagcgcc
     6121 atccagaaat taaataataa aaggaaggag attttatgac agcaaagtac aagccattcg
     6181 ataaagctga atggctccgc cttgaaaaga aggctgacga gctaagacgt ttatgcttgc
     6241 aaacagccat ctgaggtggt tcgggacaca ttgggggcgc cttgtcttcg atggacgtgt
     6301 tgacaatttt gtatcaccgt ttcatgagtc tggataaaaa caatccagag tgggaagaca
     6361 gagacagatt tgttttgtct aaaggccatg gtgctgtagg ttatgcgcca gtaattgtag
     6421 attatggttt tatgccaatc gaagagcttc agatttttaa tttgactggt tctaaaattg
     6481 gtatgcacct tgattcaaac aaagtaaagg gaattgattt ttctactggc tctttgggtc
     6541 atggaatttc tcttggtgtt ggtgctgctc ttgctgcaaa aatgaaaggt aaggattata
     6601 tgacttacgt tttatgcggc gatggagaga tgaacgaagg ttctaattgg gaaggtataa
     6661 tgagtgctgc tcagtataag ctttccaact tggttgtttt tgttgataac aataagtgca
     6721 tgattgatgg tcgtgttgaa gatgttatga acatcgaacc aattgataag aagttcgaag
     6781 cattcaactt taactgtgtt cgtgttgatg gtcacaactt caaggaaatg gatacggcaa
     6841 ttgaagcagc tatcaagaac gcaaaaacag gtgagcgccc aacagctatc atccttgata
     6901 cattcaaggg cgaaggtgtt gactacatgc gcgataatta tttgtggcac tatggtgctc
     6961 tagatgagca gaaagccgcg gatgcctttg cttcgttaga caaatattat caagagcgtg
     7021 ttgctcgcgt agaagaggag ggtaagtaat catggctggt atggtttata caagtattga
     7081 tgcggaaaaa ttaactaccg cagaatttta cggaattgct cttcgcgatc ttgcgtctga
     7141 gcacgataat gttgtagcgc ttacagcaga ccttgcaaaa tcaactaaga ttgctgcttt
     7201 tgcgaaggaa tatccagagc gcttgattaa tgttggtatt gctgagcaaa acatgtttgg
     7261 tgttgctgct ggaatggcaa agaatggctt cgttccttat acatcaacat tttctatttt
     7321 tgcttgtgct cgttcacttg accaagttca cacagacatt tgttatcaaa atgttcctgt
     7381 taaaatttta gcatctcatg ctggaacttc gtttggtcag gctggatcaa ctcaccatgc
     7441 aatcatcgat ttggctgttg cacgttcgct tccgcatatg acagttatgt gtccttgcga
     7501 tggtgccgaa gcttacaaac ttgttttggc ttcttatgac attccaggcc ctgtttatat
     7561 tcgtattaat cgtggttttg atgctgttgt ttgggatggc ggtcctgagg cagtagattt
     7621 tcagtgggat aaagctgcaa ctatgatgga cggcgacgac attacattta tcggtattgg
     7681 tgctggtgta tatcaagcaa tccaggctgc acgtatgtta aagcacgatg caggagttaa
     7741 tgctcgtgtt ttgaatatgc atactttaaa gccaattgat aaagaagcta ttcaaaaggc
     7801 agttgatgaa acaggcaaga ttgtaacaat cgaagaccac gtaattcaag gtggtttagg
     7861 atctgctgtt gctgaggttg ttgctgaata tggcaaacct tgcacattaa agatgctagg
     7921 tcatgacaat gacttttcaa caattggtct tcaggaagat ttgcttacaa ttgctggaat
     7981 cgattcgaat agcatcgaaa aggctgctgc taaacttttg ggcattagcc ttaaaactgt
     8041 tgataattgg aatgacgaat actaaggagg taagatttat ggctacatca gaagaaacaa
     8101 aatacgcaaa taaagttttc atggctggag ctcttcctct tatgaaaatt attgctaacg
     8161 aagatccaaa gttaagtgca acttttaaaa gaaagaagct aaatgctgtt tatcaagttt
     8221 cggcaatgga cgaaggtcag aagcacgctg ttcacttcgt tgtcgaaaat ggtgtttggg
     8281 atgttttcca aggtgaatac aatggtccta aaaagattga tgctgaactt gcttttgctt
     8341 cgatggaaaa aatgaacgga tttatgaaat ctgacatgaa acaacttcca tcttttaaga
     8401 ttaaaagtgt tacgaagttt gctcaattca tgaaggtatt gctgaagatg tcctcgttgc
     8461 tcactgcaga caaaccacca aaagatgttg aaactcaacg ctttttgaca aagctatatt
     8521 tttgcctgtt gtcgactgga atttcggctc taaacaaaat gggtcatcca gaaatttcga
     8581 agtgggttgc agaaagccct gacagaactt atcaatggac aattgttgga gaccctgaca
     8641 cttcagctta tcttcgtatt tgcgaaggca attcgaaggc tggtaagggc ctttatgaaa
     8701 gaagtgatcc tttctttaca atggcttttg attgtccaga aagtgcgctt ttaattttga
     8761 tggaaattgg agacatgttt gacatgacag ccaacagaca acttatcatg aaaggtggcc
     8821 cagaatttgg tgttcaattg ggcgatcaca tgacaactgt tggtcactta gcaaaatagg
     8881 atagcttatg aataaaactc aaattgcaaa aatttgcgaa gctcaacgtg cgttttttgc
     8941 gactggcaaa acgcttgacg taaactttcg tatagttcag cttaaacgtc ttcgtgaatt
     9001 tattaagcga tatgaaaatc agcttgaggc tgcaatgaaa aaagatcttt gcaaaagcga
     9061 agtcgaaggt tttttgtgtg atgttggtcc aagcattatg gaaattacgg aaactattaa
     9121 ggggctaaag aaatgggcga gaagcgagac tcattttagc ggacttttgt gttgaccaag
     9181 tgtggttaca aaagttcaca aaatgcctta tggaaattgt ctgattatta gcccttttaa
     9241 ttttcctgtg cttttaactt ttggtgtttt agctgcttgt attagtggtg gtaacaccgc
     9301 ggtgcttaag tgcagttcaa aaagttctga gagcaccaag gtaatgaaaa agcttattcg
     9361 tgatgctttt ccgcaaaaat atattacggt tattgatggc ggacacgatg ttgctgatat
     9421 gtgtttggat cagcgcttcg ataaaatttt ctacacagga agtccaaaag ttggtgttca
     9481 tgttatgcaa aaagctgcga agaacttgac tcctgtcgca cttgaacttg gtggagagac
     9541 aggaaactgg tgtgttattc gcaaagatgc taacttaaaa gatgccgcgc gtaagattgc
     9601 gtttttcaag gcttgcaata gtggacaaat ttgcattaat attaaccaaa ttgcagtcgc
     9661 gcgtgaagtt gccgagcctt ttattgaaga gttgaagaag gctttcgaaa aacagcttgg
     9721 cactaacgca atccagaacg acgagtatcc aaaactcatc actaaagcag cttatgacaa
     9781 gtgtgataat tgggttaaaa agtataagtc taagcttgta tatgggggtc aaggtgatcc
     9841 gaagactcac aagtacaatc caacaatttt atatccaatg agtgccgatg atgatatttg
     9901 taagaatgag ttgttcaatc caattttgcc gatcgttcca tttgccgata atcaaattga
     9961 caaagtatta aaaatgattg cttcgcgcga acatggttta gcactttatc tttttacatc
    10021 tgatattgct tgagccaaac gtgttatgaa aactcagcaa tttggcggag gttgtattaa
    10081 cgaagtatgt attcacttga tggttaaagg cgttccgttt aatggcactg gtcattctgg
    10141 tatcggcgcg tatcatggcg agtgaggttt ccgtgaattt actcatccaa cgactgtttt
    10201 gatgggtaag aagtacttca atctgccgct tcgtgaacat ccttataacg gtaaaaaagg
    10261 catcaaatac aaaatgatta aatctatgat gagataaaaa aataagcctg caataagcag
    10321 gcttattttt ttttatttta aatctgatta tttctttgca ggttttttgc tttggtattt
    10381 aacacaaaga ataacaccaa ttatgcaaac tattagcgtc aaccaagctc atcctgttgc
    10441 ttcgaaaata ttaggaataa tgaaaaccaa tgattgaaca acagaactta cagccaagca
    10501 gattagcata aataggaata caaaaatacc ttcaatgata aatgctgcgg tgcatccctt
    10561 cgtaattttt ttctttgcaa tacaaatgat agctgcataa gcattaaaac caacaacaac
    10621 aaggacccat aatgtaactg cagcagaacc tgctttaaca atggaaccaa cgcttgctaa
    10681 actttgtttt gcagcagcaa gttggtctgc agttggaaga gcattttgaa ttgcagcaat
    10741 aattccatta acagtttctc cgtttgcagc aacctggata cctaaaacgc tgcatatagt
    10801 attgatgtat tgagtaacat catgaagcga atcgaggtta gtattaattg ttgcaaactt
    10861 atccacaatt gcatttagcc cattaatctg agcatccaca ctaaagaata tagaaagtag
    10921 tgtttgcgga attgcaaaca aaccacttaa gaaatcgatg tgtaggcttt cgtataattt
    10981 tgttagaacc gtaatattta gccatggcat aaagaataaa ataattccta atattgccaa
    11041 gaaggcaaca taatatgcct tgtctaaatt cttaaagaaa gctccaacgc tattaagcac
    11101 atcagtaaat ttaacattct tgactgctgt tgtagctttc ttaacagatg cacttgcttt
    11161 tttctttgca gcagcggtag tttttttcgc agctgcggta gtttttttgg cagtagactt
    11221 tgcctttgtc ttcgtagatt ttgtagccat gattctccta acctaatttt ttaatttgga
    11281 atgcataaac aatgttgcta actccgaaga caattgcact gattgcagca ctaattgcca
    11341 caatagcaag tccaagaatt gggttaccca taatagatag ggcagccaaa gcaacaagga
    11401 tacctaagat taaaagtagt acccagcttc caacattcgc ttttttgatt tcaagagata
    11461 agatgatcat tgaaattcca tcaaagagga atccaaaacc gcaaataaac cataaaaagc
    11521 ttaatgcaaa agctggtctt gttagcatgc aaacacctgc aataattaca agtatgtgaa
    11581 gtgctaactt aagaccccat aaatggatgt actgcctgtc aacaatagta ataactgcac
    11641 caaaaattcc tgaaaataga aaatagatac ctgcaattac agcaactgtg gtaattgcaa
    11701 atgccatatt tccaaatagt gcaattccga ataatgtggc aagaatacct aagattagat
    11761 atagccacca acctttactc atttttccaa tttgtttagc cattttttct cctttcatag
    11821 ctgtgttaat tatagcgaat aataaaatat ctttcaaaaa aattatgcat tttagtctat
    11881 ttagatttgc ttacgccttc gccataaatt gtttttcaat cgtccttcat tgaaattgta
    11941 attcagccat atttgtctgc caagtcttga acttttttag atgtttcaag gttgccatat
    12001 tctcggtcaa gatcatctca cttaattatg aatgctagac ttttgtagtc tgggttgcta
    12061 atagtaaaat ttagcatgct tgcatcgccg gttgaatttc caaaagaaag gactggcaat
    12121 ttgccaattt cttgtgccat aattgctgcc ttattcatct tgatgctttt ttgaattaac
    12181 tcagatgtgc gaataaggtt ttcgcctggt tgatattcgt attcaagagg atcggtgctg
    12241 ccttgatttt tcgtgataac tttataatcc attccaatta tttgggaact tggaatttct
    12301 ggaaaaatgt cagatagaag cgctctgtcc aaaaatcggt ctgtgccact gcaaatgtaa
    12361 actataaaac catagtcttc taagtattta acaacttctt tcattggctc aaaaaatgcc
    12421 tctccaagat tcatgttctc aaagccatcg gcttttgtct ttttaaactg cctaacataa
    12481 ttgtagtagt cgtaaagggt cattccttcg taggctttac ctccaacttc agattctcat
    12541 ttttcataat ttgaaggaat tttgctgtcc tgtttcaggg aacgaattcc cttggccaat
    12601 tcaatttctt gcggcgtggc tttatcttta tagtcagggt cgtcaagaat tctgtgggca
    12661 agcattagtc attcaaaata tgtaggatat ttttcagaca ttaaagttcc gtccatatca
    12721 aaaactgcga ttcggtcttc gtgccttata tagtttggac ttgtttcgtc tgttacatcc
    12781 gaaacataac tttgaagagc ttgaagtggg gcagaatctt catttcatga tttgaaatac
    12841 gaaggcctgc aaaaaataaa tgctaaaacg cctacaacaa caatcacaca caaagtaatc
    12901 aatagtcctg tttttaccga tttattcatc tttttgcttt tagacataaa cacctcattt
    12961 ctcaatggaa ttatttaatt atctttatta attatatata atttgtgttg actttatata
    13021 tatatattag tgtttatagt tttgattact attaaagatt ctttatatat aataagcaat
    13081 aaaaggaggg gaactatgaa taaaacaaat ttcgccaaca atttcataat taaagttgta
    13141 ttaggcataa tctttgctgt actaattttt ttatctgtac ttgctcttgg atgttcgttt
    13201 gataaatctg cagatcttgt tgtttatgga aatatttaca ctgctgaaga tgataacgac
    13261 agtatggccg aggccttcgc agtaaaagac ggaaagtata tttatgtcgg tgataaaaat
    13321 ggtgttaagg attacatcaa ggaaggcaag actgaagtaa ttgacaggtc tagcgaaggc
    13381 atggtcatgc cgggtgcgac agaaggacat agccattact ttgggcaaag cgccatagaa
    13441 acttgtcttc ctgtttctaa ttgttctttt aacgaaatga tagatgcatt aaaggcacag
    13501 gtggctaacg taaagcctga aaagtttttt gcttttggat gaaatccttc tgatttgcac
    13561 gatactctag ctacaggcgt taatattgct gaagatcttg aaaagattgc tcctggcata
    13621 ccggtagtgc ttattgcaag ttctgcacac gatgctgttt gtaatactac tgctttacaa
    13681 aaggcaggat ttttggaaaa tactaacttt cgtgggggtg aaatttgctt agattcaaac
    13741 ggtaaaccaa gtggcttagt aaaagatgag gctgtttact atattgttga aaaggctatt
    13801 ggtaaactaa tcactgacga gcaatatctt gaggcttata aaaatgttca acaaacctta
    13861 ctgggttatg gttatacaaa tgcattagat gcctttgcta atttgtatga ttcttcggcg
    13921 acttatgaaa atctacaaaa gttagatgat gaaggtaatt taaatatgaa tgttgctgaa
    13981 tgtttcacaa ttaagagttg tgatgctgaa aactacaaat ctaaggttga tgaggttgta
    14041 gaattaacca aaaaatataa aagtaagcat actaatccag ccttcatcaa attgtttgcg
    14101 gatggtgttg ttgaatcggc tactggatgg atgtttgacc cttataagaa tgttgcaagt
    14161 ggtaaagaac atggaaatat agtatgacaa caagatgaat taaatgaact tgtcaaatat
    14221 gctaacagta agaatatcct tgtccacact catacatatg gagatgctgc gtgtaatgct
    14281 ttgttaaatg cgtatattaa ttctaataaa gaaaattcag gagagttcag gaatagcctg
    14341 gggcatgctc gtaatattaa agctgacgac ataaaaaggg cagcggaaaa taaaatttct
    14401 attgcagaaa atcttgtatg gcactcaggc tacgacgatt caaaccctaa cgatgtaaag
    14461 caaaaacaga gtgtattaag tcatatttcc gaagaagctt attacgcagg ttaccccatg
    14521 aaatcattaa tagataatgg tgttgttgtt agctcttcaa cagatgcccc agctgctgta
    14581 tattgtactg gaaacattca gaatgttatt gaggttgcaa ctacaggtat tatccccgaa
    14641 gaaaatgcga agccatttac tcccagtgaa ttattaactg taaaagatgc attgaaggct
    14701 ttgactatta atggagcatg gcaaattgga cttgaaaatg aaagaggttc catcaaggta
    14761 ggaaagtatg cagattttct attattagat aagaatatct taaattacca gggtgagcag
    14821 cttcgtacaa ttcataatgt taaaatatta aacacttatt tcgaaggcaa aaa
//
LOCUS       DBNCDC010000016        14491 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_1399_length_14491_cov_7.941466, whole genome shotgun sequence.
ACCESSION   DBNCDC010000016 DBNCDC010000000
VERSION     DBNCDC010000016.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 14491)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14491)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14491
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1399_length_14491_cov_7.941466"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="AC04VQ_02200"
     CDS             complement(<1..524)
                     /locus_tag="AC04VQ_02200"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIO9553037.1"
                     /translation="MTNLPKDLISKLTDSFVITKPQLHSSQLSSDGTKKYVLEMKSGV
                     KDEKPVFIECVAIPSLKESSNKLTVCISSQAGCPMACEFCATGQQGFVRNLSCGEIVQ
                     QVLFIQNDLGMKVSNVVVMGQGEPFLNFENLTDALDIINNPKIIGIGARKITVSTCGI
                     IPGIDKFSKIDKQYG"
     gene            680..2788
                     /locus_tag="AC04VQ_02205"
     CDS             680..2788
                     /locus_tag="AC04VQ_02205"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HIO9553038.1"
                     /translation="MAKMRDFLVKYMPVAEMKNIALLYGGTSSERDISLLSGKNVGEA
                     LTSRGYNVTYIDPKQNEDMDKLRNEKFDIAYIALHGKGGEDGAIQAELEKLGIPYTGS
                     KVQASKNAMNKAVTKEIFKRGKVSSPKYFVVNKHQKTDLEKQIKITGDDVVVKAANEG
                     SSIGLYFAKGKDEIEKAIEKAKDFDDTIIVEQRISGREFTCAVIEFDNEDKKDLVEKL
                     TFDGNNAALPVIEIVPKNEFYDFASKYDEGGSEHICPAEIDDELAQKIQKLALKAHKS
                     LECSGFSRTDILVSDEGQVYALETNTIPGMTSKSLVPDAARAVGISFEDLCELLINNE
                     LKKSVAPSPLDPNNRAAKYAIAREEAKKAKKKKIIIAAITIGICLLSIFGAWLYSFLN
                     TSFEDSDEFKIREELTESSFDEAFYMLLIGTDTREGGTYSLEDGRSDSCILVRIDPIN
                     YIVTMISIPRDTKMTDDSGTYKFNAVYAESGVKGTIKAVKQMMGVDIAHYAEISFNGL
                     TDMVDAVGGVEVDVPEEINDDQTQVYVPAGKQTLNGAQALSFSRSRKFGDGDFTRTAD
                     QRVLIDALIRKAYKMGVGEVPNLLRAAKNFVKTDLRLGDMIGLAMQFLNADKELTLYS
                     AMVPSYTSGEGGISYVITDKDALKRMMKMCENGENVLYVEINSGASVCSSRDATNLEA
                     ERQAYYQQHPDSPGKISNSE"
     assembly_gap    2803..2902
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3064..3738
                     /locus_tag="AC04VQ_02210"
     CDS             3064..3738
                     /locus_tag="AC04VQ_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIO9553039.1"
                     /translation="MGKGLKGLILGGAVGAAIGVLYAPRAGKKTREMLAKKTDALWGE
                     EAQKQGTILGEVAKTTKTAVEAGQNIFNEAQKGKFGEITREATERGQQIFNETKSYVN
                     DFTEENVRPVFAEKNDELRKKIDVARAKIASQVSQNAKASTPVRSVTPATRKAPAAKP
                     AAKPAAKPVAKPAAKKATPKKKVVKKTTAKKAAPKKTTAKKTTAKKPAAKKTTAKKTA
                     KRTTKK"
     gene            3883..4008
                     /locus_tag="AC04VQ_02215"
     CDS             3883..4008
                     /locus_tag="AC04VQ_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553040.1"
                     /translation="MFHRKPAFIALNDCTIKKDGIFAKKTNAIMKENKIDEKAIS"
     gene            4015..4398
                     /locus_tag="AC04VQ_02220"
     CDS             4015..4398
                     /locus_tag="AC04VQ_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553041.1"
                     /translation="MPVNISKEKLGYVGRVDFDAKTGKLESIGLSQGIIAGAVLGKSD
                     IKAKDIEGYSAKHQAIMLKTGASILESKKGAAEAAGKATSVVVHKVKKGAPKVIDSMQ
                     EQSDKIHDMFHEFKDEIKKGMEDNE"
     gene            4407..4964
                     /gene="frr"
                     /locus_tag="AC04VQ_02225"
     CDS             4407..4964
                     /gene="frr"
                     /locus_tag="AC04VQ_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013979122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIO9553042.1"
                     /translation="MISDVLKDTEAKMQNAIVALKNNLTQVRTGRANAAILDGILVDY
                     YGQPTPINQMAAIKTPDAHQLAIEPWDKSQLGAIEKAIAESDLGISPNNDGNAIRLPF
                     PKLTEESRKELVKTCAQYAEEARVSVRNARRDGNNGVSKTVKDDNLSEDEEHRGQDEV
                     QKLTDKYIKEVDEVFADKEKEVMTI"
     gene            5114..5767
                     /gene="uppS"
                     /locus_tag="AC04VQ_02230"
                     /pseudo
     CDS             5114..5767
                     /gene="uppS"
                     /locus_tag="AC04VQ_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013424.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
     gene            5767..6063
                     /locus_tag="AC04VQ_02235"
     CDS             5767..6063
                     /locus_tag="AC04VQ_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553043.1"
                     /translation="MKEKKQHSDLFIRLRSGLIYAIVILAGSLLGNIATTIMIAAAAG
                     ISAFEFYFMLRSDAKLPNEALGIAAAVAFPVTYFFFQMKGVIFVTAAFIIALLV"
     gene            6088..6603
                     /locus_tag="AC04VQ_02240"
                     /pseudo
     CDS             6088..6603
                     /locus_tag="AC04VQ_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013327.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
     gene            7003..7542
                     /locus_tag="AC04VQ_02245"
     CDS             7003..7542
                     /locus_tag="AC04VQ_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553044.1"
                     /translation="MLSLSAIGFFVGKNAFANEGSLQPDTDKITAKVYDDGSVSFSTC
                     NLTNVSSEIYSLDQASVSVSDEAKSVVALASSTFTINGFDGVVYKGNPDGQFREAFNV
                     APLSSGEETQLSFALEGLNKDNLMSLVDKNVFSISLKPIKVYEVNFDVQGHGIAPASY
                     LKIKEGDKIPSPIDPTETG"
     gene            7813..7986
                     /locus_tag="AC04VQ_02250"
     CDS             7813..7986
                     /locus_tag="AC04VQ_02250"
                     /inference="COORDINATES: protein motif:HMM:NF021028.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIO9553045.1"
                     /translation="MKGDGSLVVKYYYVRNSYEVSFDKNGHGIAPDKQTIKHEGLVER
                     PENPTEPGYIFED"
     gene            8161..8487
                     /locus_tag="AC04VQ_02255"
     CDS             8161..8487
                     /locus_tag="AC04VQ_02255"
                     /inference="COORDINATES: protein motif:HMM:NF021028.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIO9553046.1"
                     /translation="MGPTDSKVTPDTKNYTGFKAPEKQTVTISADGSTVVDYEYTRNS
                     YTVTFDANGHGTSPRATDVMYEDKATQPEEPQAEGYIFSGWYKESECIHEWIFAEEQV
                     TRDTTL"
     gene            8908..9171
                     /locus_tag="AC04VQ_02260"
     CDS             8908..9171
                     /locus_tag="AC04VQ_02260"
                     /inference="COORDINATES: protein motif:HMM:NF021028.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIO9553047.1"
                     /translation="MVISADGLTVVDYLYTRNSYTVSFDSNGHGSAPETETVKFEDFA
                     IAPTSEPTETGYTFNGWYKESECSNQWDFSKDKVIEDTTLYAK"
     gene            9196..9306
                     /locus_tag="AC04VQ_02265"
                     /pseudo
     CDS             9196..9306
                     /locus_tag="AC04VQ_02265"
                     /inference="COORDINATES: protein motif:HMM:NF021028.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9424..9624
                     /locus_tag="AC04VQ_02270"
                     /pseudo
     CDS             9424..9624
                     /locus_tag="AC04VQ_02270"
                     /inference="COORDINATES: protein motif:HMM:NF021028.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
     gene            9856..10053
                     /locus_tag="AC04VQ_02275"
     CDS             9856..10053
                     /locus_tag="AC04VQ_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553048.1"
                     /translation="MYYGYQDTVNGKILYLAGCSTQNATINCNAALSSTQESDCGWND
                     QRENITDVIVETHIYPTSTSY"
     gene            10069..10806
                     /locus_tag="AC04VQ_02280"
                     /pseudo
     CDS             10069..10806
                     /locus_tag="AC04VQ_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024702.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
     gene            11263..11397
                     /locus_tag="AC04VQ_02285"
     CDS             11263..11397
                     /locus_tag="AC04VQ_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553049.1"
                     /translation="MSGEEVYGQTGIPNIFKEFEKAQYTKKQNEGTTWDSFNKNKAYV
                     "
     gene            11689..12795
                     /gene="dxr"
                     /locus_tag="AC04VQ_02290"
                     /pseudo
     CDS             11689..12795
                     /gene="dxr"
                     /locus_tag="AC04VQ_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024684.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
     gene            12806..13873
                     /locus_tag="AC04VQ_02295"
     CDS             12806..13873
                     /locus_tag="AC04VQ_02295"
                     /inference="COORDINATES: protein motif:HMM:NF019307.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HIO9553050.1"
                     /translation="MKKFMSKKLMLVSLASTLLFSLFIVSACVQQNKIQEGKFPNTVA
                     ENAKVADQYVGVANEMSDFAIKEFTENISKIPRKSKNCEQIHKYLYNWATQNNLNVNI
                     DQSGCVYWDVPATSGCENYPNIILQAHTDMVHTTNDESIDMNTTPIDVVFDESINGLH
                     SKDYKTNIGADDGEGLVTMMALSTNKNFKHGPLRMLCTYDEEITMEGAIRLSPEVLNA
                     NYLLNVDAGPVGSACVSSAGILRVGFNNQYQLAPTNKNTILEVELKDLAGGHSGVDIA
                     KNRLCANVCASQILQKLVDENIEFNLINMYGGEGPNIISSKAYFSIAINESDVENAKN
                     IIENEFNSQKNGHNDDKNAYV"
     gene            13934..14431
                     /locus_tag="AC04VQ_02300"
     CDS             13934..14431
                     /locus_tag="AC04VQ_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553051.1"
                     /translation="MKFINELPHGVREMSEVRPDMPAASSNFGTITLYNGQLKVVVHS
                     RSNKQNANDELDKKFVELSKQYGIAYSVDNKYAAWENSEDKTLSNLFAESFKNTSGIE
                     TVDYISHAGLECSRLYEKNPNLKMLSIGMDVADEHMVTETLYTKSMPAFFAAFIYFLE
                     NANQL"
BASE COUNT         4702 a         2711 c         2991 g         3987 t
ORIGIN      
        1 ccgtattgtt tgtctatctt agaaaactta tcaataccag gaataatacc gcaagttgaa
       61 acagtgatct tacgcgctcc aatcccaatt atttttggat tgttgataat atccaaagcg
      121 tctgttaagt tttcgaagtt aaggaaaggc tcgccttggc ccatgacaac aacattagaa
      181 actttcatgc ccaaatcatt ttgaatgaat aaaacttgtt gaacaatctc tccgcaacta
      241 aggtttcgaa caaatccttg ttgtcctgtc gcacaaaatt cacaagccat aggacatccc
      301 gcctgacttg aaatacaaac agttaatttg ttagagcttt cttttaacga aggaattgca
      361 acacactcaa taaagactgg cttttcgtct ttgacgccag atttcatttc taaaacatat
      421 tttttcgtgc catcagacga taattgtgaa gaatggagct ggggcttcgt aatgacaaaa
      481 ctatcagtaa gtttagaaat taaatcttta ggtaaatttg tcattaacga aaaatcatcg
      541 gtctctcgcc cataaactca ttccaaaatt tgctcaagcc tataagacgg ctgcttgttc
      601 ttctcgagaa aagcacgcaa atcttcgaac cgcaaattat taaacgaaac tttattcata
      661 ggtgagcatt ataccttaaa tggcaaaaat gagagatttt ttggtaaaat atatgcccgt
      721 ggctgagatg aaaaacatag ccctactcta tggcggaacc agttcggagc gcgacatctc
      781 ccttctttcg ggaaagaatg ttggcgaagc gcttacatcc cgaggttaca acgtcacgta
      841 tattgatcca aagcaaaacg aagacatgga caagcttcgc aatgaaaagt ttgacatcgc
      901 ttatattgct ttgcacggta aaggtggcga agacggggct atccaggcgg aacttgaaaa
      961 acttggtatt ccttataccg ggtctaaagt tcaagccagc aaaaacgcca tgaacaaagc
     1021 cgtcactaag gaaattttca agcgcggaaa agtttcttcg ccaaaatatt ttgttgttaa
     1081 caagcatcaa aaaactgatc ttgaaaagca aatcaaaatt accggcgacg atgttgttgt
     1141 taaggctgct aacgaaggca gcagcatcgg tctttatttt gcaaaaggta aagatgaaat
     1201 tgaaaaggca attgaaaagg ctaaggattt cgacgacaca atcattgttg agcagcgtat
     1261 ttctggcagg gaatttactt gtgctgtaat tgagtttgac aacgaagaca aaaaagacct
     1321 tgttgaaaag ttaacatttg acggcaacaa cgccgctctt cctgtcatcg agattgttcc
     1381 gaagaacgaa ttttacgact tcgcttctaa atatgacgaa ggcggatctg aacatatttg
     1441 cccggccgaa attgatgatg agcttgctca aaagattcaa aaacttgcac tgaaggctca
     1501 taaaagcctc gaatgcagcg gttttagtag aactgatatt ttggtaagtg acgaaggtca
     1561 ggtctacgct ctcgaaacaa atacaattcc aggaatgaca agcaagagtt tggttccgga
     1621 tgcagcacgt gctgttggaa tttcgtttga agatttgtgc gagctactaa ttaataatga
     1681 acttaaaaag tcggtcgccc cttcgccgct tgaccctaat aatagagctg caaaatatgc
     1741 aattgctcgc gaggaagcaa aaaaagccaa gaaaaagaag ataattattg ctgcaatcac
     1801 tatcggaatt tgtcttttga gtatttttgg cgcgtggctt tacagctttt tgaacacatc
     1861 gtttgaagac agcgatgaat ttaagattcg cgaagagttg acagaaagca gctttgacga
     1921 ggcattttat atgctcttaa ttggcacgga cacgcgcgaa ggtggaacgt atagtttgga
     1981 agacggacgt tctgacagtt gtattttggt tcgcatcgac ccaatcaact atattgttac
     2041 gatgatctca attccccgtg ataccaaaat gactgatgat agcggaacgt ataaatttaa
     2101 tgcggtctat gccgaaagtg gagttaaagg aacaattaaa gctgtaaagc agatgatggg
     2161 agtagatatt gcccattacg ccgagattag ttttaatggt ttgaccgaca tggttgacgc
     2221 ggttggtggt gttgaagttg acgttccaga agaaattaac gatgatcaaa ctcaggttta
     2281 tgttccagcc ggaaagcaaa ccctaaacgg tgcgcaagcg ctttcgtttt ctcgttcgcg
     2341 aaaattcggc gatggtgatt ttacaagaac cgcagatcag cgcgtgttaa ttgatgcgct
     2401 aattcgcaaa gcatataaga tgggagtcgg cgaagttccg aaccttctgc gtgcagctaa
     2461 aaatttcgtt aagaccgatc ttcgtttggg agacatgatt ggtcttgcga tgcaattctt
     2521 aaacgccgac aaagaattaa ctttgtattc ggcaatggtt ccgtcctata cttctggcga
     2581 aggcggaatt tcctacgtta ttactgataa ggatgcactt aaacgcatga tgaagatgtg
     2641 cgaaaacggc gaaaacgttt tatatgtcga gatcaactcg ggcgcttcgg tatgttcgag
     2701 cagagacgca acgaatctcg aagccgaacg tcaggcatat taccaacagc atcctgattc
     2761 tcctggcaaa atctccaact ccgaataacc tgtgagggcc ggnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnagcatcct gattctcctg gcaaaatctc caactccgaa
     2941 taacctgtga gggccggggg cagcgggagt tcagcgggag ttcattattt tttatttttg
     3001 tttattagta tttatttaat gttataataa aaaatccaat ttgctataaa acaaggagga
     3061 aaaatgggta aaggtcttaa aggattaatt cttggtggcg ccgttggtgc tgcaattgga
     3121 gttttatatg caccacgagc aggtaaaaag actcgtgaaa tgcttgcgaa aaagacagac
     3181 gctctttggg gagaagaagc tcaaaagcaa ggaacaattt taggtgaagt tgcaaaaaca
     3241 acaaagactg ctgtcgaagc tggacaaaac attttcaacg aagctcaaaa aggtaagttt
     3301 ggcgagatca ctcgcgaagc tacagagcgc ggtcagcaaa ttttcaatga aacaaaatct
     3361 tatgtaaatg acttcaccga agaaaacgtt cgtcctgttt ttgccgaaaa gaacgatgaa
     3421 cttcgtaaaa agattgatgt tgcacgtgca aaaattgcct cgcaagtttc tcaaaatgcg
     3481 aaggcatcga caccggttcg cagcgtaact cctgctacaa ggaaggcccc tgctgctaaa
     3541 ccagctgcaa aacctgctgc aaaaccagtt gcaaagccag ctgccaaaaa ggcaactcct
     3601 aagaagaagg ttgtaaagaa aacaactgcg aagaaagcag ctccaaaaaa gactactgca
     3661 aaaaagacaa cagcaaagaa accagcagca aagaaaacta cagctaaaaa aacagcgaaa
     3721 cgcactacta agaaataatt cgctgtgatt agttctaaga acttatacaa caaacgtgtg
     3781 tacgttgacg gaagcaaaaa aagtttaggt cgcgtgcgtg cgtttctttt tatagagaaa
     3841 aattgcatcg gttttaaaat caaacgaccc gatttccttt gaatgtttca cagaaaaccc
     3901 gcatttattg cgcttaatga ttgcacaata aaaaaagatg gaatttttgc aaaaaaaact
     3961 aacgcaataa tgaaggaaaa taaaattgat gaaaaagcaa tctcatgatt agaaatgcca
     4021 gtgaatattt ctaaagaaaa attaggatat gttggcagag ttgacttcga tgctaaaact
     4081 ggaaagctcg aaagcatcgg actttcgcaa ggaattatcg ctggcgcagt tttaggcaag
     4141 agtgatatca aagcaaaaga catcgaagga tattcggcaa agcaccaggc cataatgcta
     4201 aaaaccggtg caagcatttt agagtcgaag aagggagcag ccgaagcagc gggcaaagcg
     4261 acttcggtag ttgttcataa agtgaagaag ggcgctccaa aagtgataga ctctatgcaa
     4321 gaacagtctg ataaaatcca tgatatgttt catgaattta aagatgaaat taaaaaagga
     4381 atggaagata atgaatagga gtaaaaatga ttagtgatgt tttaaaagat actgaagcca
     4441 aaatgcaaaa tgctatcgtc gcgctaaaaa ataatttaac tcaagtgcgc acgggtagag
     4501 caaacgccgc aattttggac ggaattttgg ttgattatta cgggcagcca actccaatca
     4561 atcaaatggc agcaatcaag actccggatg ctcatcagtt ggcaattgaa ccttgggata
     4621 aaagtcaact tggcgcaatt gaaaaagcaa ttgccgaaag cgatcttgga atttcgccaa
     4681 acaacgatgg caacgcgatt cgtcttccgt ttccaaagct taccgaagaa agccgcaagg
     4741 aacttgttaa gacatgcgct caatatgccg aagaagctcg tgtttctgtt cgaaatgcgc
     4801 gccgcgacgg caacaatggc gtttcaaaaa ccgttaagga tgacaattta agcgaagacg
     4861 aagagcatcg cggacaagac gaagtccaaa agttgaccga caaatacatc aaagaggttg
     4921 acgaagtctt cgccgataaa gaaaaagaag tcatgaccat ctaactcgat ttccccgggg
     4981 ccaggactcg atttactcgt ggcatttact cgattttatg aaaaatttga ccgaaatatt
     5041 ccccaatgtt ccaagcgata ttgacatcgc ggagttggac ttcgaaaaaa ttccgcaaca
     5101 cgttgcaata atcatggacg gcaacggccg atgggcgaag gctcaaggca agccgcgact
     5161 tttcggccac aaagctggcg ttgatagcgt tcgcgaagtt gttcgcaccg caagtgacat
     5221 cggaatcaag catttgaccg tctattcttt cagcaccgaa aattgaaatc gtcccgaaga
     5281 agaagtttcg ggcctcatgg atcttttcgc gaaatcgatc cttgcagaaa tggacgaact
     5341 ccacgcaaac aacgtccgcg ttcaaatttt gggcgacatt tcggcgctgc cggacaaaac
     5401 tcgcgaagca ttcgaagccg gcgtcgagtt gatgaaaaac aacactggaa tgactttgct
     5461 cccggcggta aattatggca gtcgggatga aattcttcgt gcggttgatg cgttcgcgca
     5521 aaactacgaa ggcaaggcgc ccaccgaaga agatttttcc aagcttttat atactaacga
     5581 agtcccagat ccagagcttt taattcgcac ctctggcgaa atgcgcgtgt cgaacttttt
     5641 actttggcaa atcgcgtatt cagaatttta cgtcacggac gttttgtggc cggactttga
     5701 cagatacgaa tttttacgcg cgctgattgc gtttcaaaag cgggaccgtc gcttcggagg
     5761 tgtgtagtga aagaaaaaaa gcaacactcc gacctattta taaggttgcg ctccggccta
     5821 atttacgcaa ttgtgatttt ggcggggtcg ctactgggaa acatcgcaac gacgatcatg
     5881 attgccgccg ccgccggaat ttcggccttc gaattttact tcatgttacg aagcgacgcg
     5941 aagctgccca acgaagcatt gggtatcgcc gcggccgttg cgtttccagt cacatatttc
     6001 ttctttcaaa tgaaaggcgt gatttttgta accgccgctt ttatcattgc gcttttggtt
     6061 tgatacgttt tttgaaggca cgcgcgaatc gccgatgttt gcattagctt tttcggagcg
     6121 atctacacag gctttttgct aacgtgtttg ctcgcgcttc gcatggcaat tccgggcgac
     6181 gaaatttggg gaggcgtctt cgtaattttc ttgctcacaa cagtttcggt caacgacggc
     6241 tttgcatatt tgttcggcaa agcttttggc aagcatagac tggcgccgca catctctccg
     6301 aagaaaactt gagagggttt tatccctgcc ttgatttgtt caaccgcaat ttggcttgtg
     6361 cttttgattt ttccgggcgt tcaaattgaa gtttggcagt gtattgtttt cggattaatt
     6421 tgcggagttg ccggagtcat tggagatttg gtcgaaagcc gcatgaaacg caattcaggc
     6481 gtcaaagatt cgggcactct tatgcctggt cacgggggcc ttcttgaccg ccttgactca
     6541 atctttttag tctcgctaac ttgcgccctc ttaatgttca gcttcaactg catccctttt
     6601 taatatgaga aacgatgtta aaaacaaata agtttgctgt ataaagtatt caatatataa
     6661 tataattaac gtgcatatta agtactctta aacaacaatg aggactttat ggactatata
     6721 aatttggcaa taaattttgt ttctgcatct gcagatgtag agaatacttc agcagcttta
     6781 acttttgatg ccactaactt tgtatgaata attcctctag ttattctttc agtctgcttt
     6841 gcctcatgtt tgttttatca aagaaagaag gctttttcta cttgaagcgt tttaggtgct
     6901 aagaacagca ttaaaaatgc aaaacatgct aataatatac attatgagac ttttagtagc
     6961 aattcaagaa ttagcaaatt gataattatt gcaataataa tcttgctctc tttgtctgct
     7021 attggattct ttgtaggtaa aaacgctttt gcaaacgaag gctctttgca gccagacaca
     7081 gacaaaatta ctgcgaaagt ttatgatgat ggctctgtgt ctttttcgac atgtaattta
     7141 actaatgtat ctagcgaaat ttatagttta gatcaagcat cagtttctgt ttctgacgag
     7201 gcaaaaagcg ttgttgcgtt agcatcttcg acatttacta taaatggttt tgacggggtt
     7261 gtctataaag gaaaccccga tggtcaattt cgtgaggctt ttaatgttgc gcccctttct
     7321 tctggcgaag aaacacagct tagtttcgcg ttagaaggcc taaacaaaga caacttaatg
     7381 tctttggttg ataaaaatgt tttttcaatt agccttaagc caataaaagt atacgaagta
     7441 aattttgatg ttcaaggtca tggtattgct cctgcttcgt acttaaaaat taaagaagga
     7501 gacaaaattc cttctcctat agaccctact gaaactggat gaatttttga cggttgatat
     7561 aaagaaaaag attgtataaa tcagtggctt tttgatactg acactgttac agaaaacaca
     7621 accttatatg ccaagtgaac tccaagaacg gatacgcctt atacagttga gcattataag
     7681 caaaatcttg acgatacgtg accagcgcaa ccttttgaaa ccgaccactt aacaggaacg
     7741 actgggtcgg aagtaacacc tgaaactaat tattacgaag gttttacagc acctcaagtt
     7801 gagcctattg ttataaaagg cgacgggtca ctagtagtta aatattatta tgtaagaaat
     7861 agttacgaag tttcttttga taaaaatggt cacggtattg ctcccgacaa gcaaactata
     7921 aaacacgaag gtcttgttga aaggccagaa aatcctacag aacctggtta tatttttgaa
     7981 gactgataca aagaacctga atgcacaaat aaatgaaact ttgaaaacga taaaatttta
     8041 gggaaaacaa tattgtatgc caattgagaa attgaccaaa atacgtcgta taaagttgag
     8101 cattttaaac aaaaaatcga tggtttttat tctgatattc cagatgatgt agacgaatta
     8161 atggggccta cggactctaa agttacacct gatactaaaa attatacagg gtttaaggct
     8221 ccagaaaaac agactgtaac aatttctgca gatggctcta cggttgtaga ttatgaatac
     8281 acaagaaatt cttacactgt cacttttgat gctaatggtc atggcacctc cccgcgagca
     8341 actgatgtta tgtatgaaga caaagctacg caacccgaag aaccgcaagc agaagggtat
     8401 atatttagtg gttggtataa ggaaagtgaa tgtatacatg agtggatttt tgctgaagaa
     8461 caggtaacaa gggacacaac actttgagca aaatgaactg aaaagtccgg ttattctgtt
     8521 aaatatgatc ctgatggcgg atctgatgtt tcaccattaa caaatcttag ctggacttct
     8581 gctaatcttt taccagcaga aaatcctcaa aaacaaggat atacttttag tggatgattt
     8641 tatggtagtg ataaaagtac tcaagctaca tcttatacta aatattgcga tatagcttct
     8701 tcggcagaag aagatgaagt tacaatatac gctaaatgaa gccctaatga aaatactccc
     8761 tataaagtta ggcatttcaa gcaaaatatt gatggaacat accctaaaga tcctgctgac
     8821 attgactcat taagtggcac tacaggaact gaagtaactg ctgaaactaa aacttaccat
     8881 ggatttacgt ctccacaaaa attaacagtg gttatttcag ctgatggatt aactgttgta
     8941 gattatttgt ataccagaaa ttcttataca gtttcatttg actctaacgg acatggatcg
     9001 gctccggaaa ccgaaactgt caaattcgaa gattttgcaa ttgcgcctac aagcgaacct
     9061 actgaaacag gatacacatt taatgggtgg tataaggaaa gtgaatgtag taatcagtgg
     9121 gatttttcta aagataaagt aattgaagat acaactcttt atgctaaatg aactgcaaat
     9181 acatatacgg ttacatttga cgctaaaggg catggcacca atccaccgcc tacagctgta
     9241 acatatgatt ctgcttatgg cgaattgcct gtactaacag atgaaggata tattctggct
     9301 ggatgatatt tagatgaatc attttcagaa ggtagtaaag tggaaactga tactgttgta
     9361 aaaacagctg aaaatcatat cttgtatgca aaatgagagg aacgcaccgg gactgttact
     9421 tttgtaacta atggccatgg cgggtctaca ccacagccaa ttaatgttat ctatgataaa
     9481 gcttatggcg atctccctag ccttagtgaa aatggctata cgtttggagg ttggtattta
     9541 gaccaagaat ttactccaga gaaaaaagtt gaatcaacaa ccattgtaaa aactgctgga
     9601 gatcattatt tatacgcaaa atgaaccgca aatacgtata tagtgtcttt tgataactct
     9661 gggcatggag ggatcacacc tgcctctatt cgagttacat ttgattctgc ctatggactc
     9721 cttcccgtgt taagcgatgc caattataaa ttttgttgct gaaaggacca aaatggatca
     9781 gtagcaaata ttgatactat ttataaaatt gcaagtgatt caacgctcac tgctacttga
     9841 accgaaaatg cacagatgta ttatggctat caagacacag ttaatggtaa aattctttat
     9901 ttagctggct gttctactca aaatgcaaca ataaattgta atgccgccct gtcttctact
     9961 caggaatctg attgtggctg gaacgatcaa agggaaaaca ttacagacgt tattgttgaa
    10021 acacatattt atccaacttc tacttcctat tgatttaata gtttcagtaa ttgcacatca
    10081 attaattgcc tgaatttgaa tactacaaat gttacaacca tgaaaaatat gtttgcttcc
    10141 tgccaaaatc tagccacaat agatgtttct aattttgata catctaaagt ccaaaatatg
    10201 agtaggatgt ttggtcaatg tttaagttta gaatctctta gtttatcaaa ttttaacaca
    10261 tcaagtgtag aatctatgga ctatatgttt tttgctggtt caaaaattac tgaacttgat
    10321 ctttcgaatt ttgatacttc aaatgtaaaa acaatgcttt caatgtttaa tttttgtgaa
    10381 tctctaacta ccttgaatat atcaagtttt aacacttcaa aagttacaga tatgtcacgt
    10441 atgtttgctt attgtgaaaa attaaaaaca atttgtgcta gtccatcttt taacacagat
    10501 gaggttaaga aagatgacga tatgtttgtt tcttgtacca gtttagaagg ttctaatgga
    10561 acaaaatatg attcaagcca tgttggaaaa gaatatgcaa ggattgatgg attaaatagc
    10621 aaaccaggat atttctcaac accaacaatt aattaccagg tttgtatttg gggcatcggt
    10681 caagataaat gtggcgttga tggttccgat accgcaaatt taacttttgg ccctgcaact
    10741 ggagagactt cacaatgttt agatgttgac ggtcatcatg aacattgtat tcataatgaa
    10801 agctgagaga caataattaa caattgcaat agtggacatg ccggttgata tcacaattgt
    10861 ttagaaaatg gctgtacaaa aagtgtacca attattctta attctgacat tggaacaaat
    10921 cctacaggta aagaactatg ctctattctt caacaaacaa attatagagg atacctttta
    10981 agtaagtatg ttaaatgaaa cgatagttct agttcttcag attgtgaaag cgcaaattga
    11041 gcaacaagca gaatacgtgc tactttgaat ggcaaaacag gaaatgagtc aatatatgct
    11101 ggagaagact gtcttgatag ctcaaattgt ctattgtctt gctttccggt aaatttgcgt
    11161 aatgctatca agcctaaata ttatcaagtt cctacaactt ggagtataga agacccttac
    11221 tctatagaga cctctgtatt tgtttccgac aagctttgat taatgagtgg cgaagaagtt
    11281 tatggccaaa ctgggattcc aaacatcttc aaagagtttg aaaaagcaca atatacaaaa
    11341 aaacaaaatg aaggaacaac atgggactct tttaataaaa ataaagctta tgtttgaagt
    11401 acaagtgact ggacctctga gttttggtgg ctccgttcgc cttatcgcaa ttcacagtac
    11461 tctgcgggct atgtgagtac cagcggctac tgatgcgact actctgtcta caatactgga
    11521 tgtgggatct cgccgtgctt ttgtctttaa ggaatcttac tctctctggc gctatgcgcc
    11581 agattagaga gtttatcaaa caatacaaaa attaattaaa ataaataata aaaataaaat
    11641 ctcagaaccc ccgaccccga gatatggttg agatgttata atatttggat gggaaaaatc
    11701 aggaacatag ttgttttggg agcgacgggg tcgatcggtt cgcagacttt ggatgttgcg
    11761 cgccagcatt ctgacaagtt gaaagttgtt ggccttgttg cgcattcttc gaaggaaaag
    11821 ttggaagccg cagcgcagga attcggcgtt tctaacacta tattagtaag cgatcaaaac
    11881 tccgaagccg aggcaaccgc caagatttca aaattaattg aaaatgacaa cgttgacatt
    11941 gttgtaaatg caatgagtgg agccgctgga ttaaccgcaa gttacaccac gctaaaaaat
    12001 aataaacgcc tggcacttgc gaataaagaa agtttagtcg tgggtggaga tttattaatt
    12061 ccaatagcaa aaaataatct cttgccaatt gatagtgaac acggagcaat ttttcaatgt
    12121 ttgacgggtg aagaaaaatc aaacataaat aaaattcact taactgccag tggtggtcct
    12181 ttttatggaa aaacaaaaga ggaattggaa aacgttacat ctaaaggtgc actgaaacat
    12241 ccaacgtgaa acatgggacc aaaaattacg atagattcgg caacgttaat gaacaaaggg
    12301 cttgaagtta ttgaggcgca tcacttattt ggcgtaagtt atgatcaaat taatgttgtg
    12361 gtccaacgcc aaagcgcaat tcacagtatg gttgaatata acgatggaag tgttaaggcg
    12421 catcttggaa caacagacat gcgcattcca attcaatacg ctctaagtta tccagagcga
    12481 tgggaaacac ctgctccacg catggatttt caaaacttgg aaagcctgga cttcggcaag
    12541 ccagattgtg aaactttcaa gtgtttgcac cttgccttcg aagccggacg tgccggaggt
    12601 acaattccat gcgttatgaa tgcagcaaat gaagttgccg ttgcaaaatt tttaaacgac
    12661 gatattaaat ttttacaaat tgctgaaatt gtagaaacca caatgaataa atttaaacca
    12721 gaaaaggttg aaagtttaga acaattaata aaaattgatt cagaatcacg aaaaattgca
    12781 accgaattaa tttaaaggag tgccaatgaa aaaatttatg agcaaaaagt taatgttggt
    12841 tagtttggct tcgacgctat tgttttcgct ctttattgtt tctgcatgtg ttcaacaaaa
    12901 caaaattcaa gaaggcaaat tcccaaacac tgttgcagaa aacgcaaaag tcgcggatca
    12961 atatgttggc gttgcgaacg aaatgtcgga ttttgcaatt aaagagttca cagaaaacat
    13021 ctctaaaata cctcgtaaat caaagaattg cgagcaaatc cacaaatatc tttacaattg
    13081 ggcaactcaa aacaacttga atgtaaacat tgatcaaagc ggttgtgttt attgggatgt
    13141 cccggcaact tcgggttgtg aaaattatcc aaacattata cttcaggctc acaccgacat
    13201 ggttcacaca acaaacgacg aatcaatcga catgaataca actccaatcg atgttgtttt
    13261 tgacgaaagc atcaacggcc tccattctaa ggactacaaa actaacattg gcgccgatga
    13321 cggcgaaggc ctcgtaacca tgatggcact ttccacaaac aagaatttta aacacggtcc
    13381 gcttcgcatg ctttgcactt atgacgaaga aattactatg gaaggcgcta ttagattgtc
    13441 gcccgaagtc ttaaacgcca attatttgtt gaatgttgat gcgggtcctg ttggttctgc
    13501 gtgcgtttct tcggcaggaa tcttgagagt tggttttaac aaccaatatc agttagcccc
    13561 tacaaataaa aacacgatcc tcgaagttga gctaaaagac ctggcaggag gccactcagg
    13621 agtagacatt gcgaagaaca ggctctgtgc aaacgtttgc gcatctcaaa ttttgcaaaa
    13681 gcttgtcgac gaaaacatcg aattcaacct aattaacatg tacggcggag aaggccctaa
    13741 cataatttct tcgaaggcat atttttcaat tgcaattaac gaatctgatg ttgaaaatgc
    13801 aaaaaacata atcgagaatg aatttaattc acaaaaaaat ggccacaacg atgataaaaa
    13861 tgcatatgtt tgatccaacg ataaaaaaga atcaaacatt tttgcactct caataaacga
    13921 tactaaaacc gtagtgaaat ttattaacga attgccacac ggcgttcgtg aaatgagcga
    13981 ggttaggcca gacatgcctg cagcgtcttc gaattttgga acaatcacgt tatataacgg
    14041 gcaacttaag gtagtcgttc attcccgaag caacaaacaa aacgccaacg atgaacttga
    14101 caagaaattc gtggaacttt ccaagcaata tggcattgca tatagtgttg acaacaaata
    14161 tgcagcatgg gaaaactccg aagacaaaac tttgagcaac ttgtttgccg aatcgttcaa
    14221 aaacacaagt ggaattgaaa cagtcgacta tatttcgcac gccggtcttg aatgtagcag
    14281 gctttatgaa aagaacccaa atctaaaaat gctatcaatt ggcatggatg ttgcagatga
    14341 acacatggtc acagaaactt tatatacgaa gtcgatgcct gcgtttttcg cagctttcat
    14401 ctatttcctc gaaaacgcca atcaacttta aaaaggtgta attcatgcct ggcaataatt
    14461 acaccttttg gtgcatttca tgcctggcaa t
//
LOCUS       DBNCDC010000017        14331 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_1430_length_14331_cov_7.010577, whole genome shotgun sequence.
ACCESSION   DBNCDC010000017 DBNCDC010000000
VERSION     DBNCDC010000017.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 14331)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14331)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14331
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1430_length_14331_cov_7.010577"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            44..158
                     /gene="rrf"
                     /locus_tag="AC04VQ_02305"
     rRNA            44..158
                     /gene="rrf"
                     /locus_tag="AC04VQ_02305"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            238..615
                     /locus_tag="AC04VQ_02310"
     CDS             238..615
                     /locus_tag="AC04VQ_02310"
                     /inference="COORDINATES: protein motif:HMM:NF025384.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HIO9553052.1"
                     /translation="MKDIEKRNIAACVVFSIITCGIYSLYWIYKMNEEINIISKHEDD
                     TSGGLVILFGIITCGIYYVYWAYKMGDKLKEYYTKKKSNEGNDLPVLYLILMIANYVA
                     CGICTIICYCLMQDRINKIVTKK"
     gene            746..814
                     /locus_tag="AC04VQ_02315"
                     /pseudo
     CDS             746..814
                     /locus_tag="AC04VQ_02315"
                     /inference="COORDINATES: protein motif:HMM:NF022281.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
     gene            complement(988..1131)
                     /locus_tag="AC04VQ_02320"
     CDS             complement(988..1131)
                     /locus_tag="AC04VQ_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553053.1"
                     /translation="MEFHPIANDSFKNSLTMMALLQANGITGKDLLSESKRLHIPYKD
                     LRY"
     gene            complement(1144..1434)
                     /locus_tag="AC04VQ_02325"
     CDS             complement(1144..1434)
                     /locus_tag="AC04VQ_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013540.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIO9553054.1"
                     /translation="MLRQRLGANVYAYRKYFNLSQETLSEKSKINISQIGNIENCRIS
                     TGIDLIEKLATVFRVDPCLLIARPLLKMPGTGLKSYSIVPTFFTEGVAAYAF"
     gene            complement(1497..2009)
                     /locus_tag="AC04VQ_02330"
     CDS             complement(1497..2009)
                     /locus_tag="AC04VQ_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9553055.1"
                     /translation="MSDEISGALTIFRTYFIMIFAAIFSGMIADKIFHSTLKWYRVAT
                     ICMAISTAIFILVGFSDARAVFGIPNNIVLIAVISILPGIFSTMIYAVQWAILSEINL
                     PAKVAGTATGLASMIVFSPDIWLNTGIGAILDATTQNPEAGYLVIFGILIAVSLVVFL
                     LCHYLLKRNK"
     gene            complement(2094..2432)
                     /locus_tag="AC04VQ_02335"
     CDS             complement(2094..2432)
                     /locus_tag="AC04VQ_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553056.1"
                     /translation="MPIATTFVFWSSLVKAIRLTGGEDDQGRCFGIYFAANAIFSMIV
                     SSSNMFMYTQFSGEGNGIMGVTISVAFFIGVAMLAITILFKEKNLQMPKSDESYKRED
                     IKAVLKNGTV"
     gene            complement(2439..2762)
                     /locus_tag="AC04VQ_02340"
     CDS             complement(2439..2762)
                     /locus_tag="AC04VQ_02340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9553057.1"
                     /translation="MKKYLTLFALGLAGGVMYIYPYIRYVFQNPLNETLGINDTQAGL
                     LLTVYGIVSIILYIPGGILADKLRAKKPLLMSMGWALVLLIGFAIVLGLHLGDKTPYW
                     IALGV"
     gene            2812..3423
                     /gene="nadD"
                     /locus_tag="AC04VQ_02345"
     CDS             2812..3423
                     /gene="nadD"
                     /locus_tag="AC04VQ_02345"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIO9553058.1"
                     /translation="MSKKIKNLGIIGGTFDPIHYGHIGCAEAAANAFDLDEVWFMPAN
                     HPNFKQDKNVNDIKDRLKMCEMALRAYGNEKFKVSDLEANRDGVTFTSETLEILKNDY
                     PDTNIFFITGTDAVFALPSWHNYEEILELATIIAVTRKGYHEPNAEQEKFLEKYADKI
                     KLVEADVIDISSSQIRQRLWNRQDVSDYVPSSIFNYMLNNNLY"
     gene            3599..4780
                     /locus_tag="AC04VQ_02350"
     CDS             3599..4780
                     /locus_tag="AC04VQ_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-rich domain-containing protein"
                     /protein_id="HIO9553059.1"
                     /translation="MLVAIFATMYFGKALAPGGDMQGGQGGPGEAMEQPQDQGGTPPT
                     KPDGDSQSQGSTTQPPSIPEGGGYAGSSSKGDSELPQNSQSTPPAKPEGESNSQQGGQ
                     GGTPPDMPSGGMQQGGGMQQGEMPQGEIPQGNSEEIGEAGWTKIALYGAESLVIALLV
                     AYLIISQCNKRKFKESFKAGKSKAVFIVVTVAITAVGGCGLAYVSTEIDGLAAPQTGQ
                     SSSAEYKAVKTISENTNLDGGSYESTESDTLAVLVMNKINATLKNFELTKTGSSNGGD
                     NTSFYGTNSGITAKDCANLIIEGAKILTDASGANGVFSFGGTAVSGVQTGTGDGTEVT
                     VRDSEITTKQDNSGGVMLTGGGILNVINCVIETFGTSSAALRSDRGGGDFNVEGGTYK
                     T"
     gene            4838..5458
                     /locus_tag="AC04VQ_02355"
     CDS             4838..5458
                     /locus_tag="AC04VQ_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553060.1"
                     /translation="MQSYASEGVVIEGKNSVNLEGCHLFDQNSKLNGQSTTYKNIFLY
                     QSMSGDSAEGTSKFSAKGSTLETSKGDTFYVTNTKSVITVEDCQVINNDTSGNLLRAK
                     ADSWGNSGQNGGDVEFNVTKQSLTGNIVIDAISKLAFGLTNGSYWEGTLNGDNASQNI
                     SLKLDRSSKIKLTGDSYVTSLDDEDTSYSNIDFNGHKLYVNGTAIN"
     gene            5566..6948
                     /locus_tag="AC04VQ_02360"
     CDS             5566..6948
                     /locus_tag="AC04VQ_02360"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015760304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HIO9553061.1"
                     /translation="MPKKVYVMGHKNPDNDSICSAVAYAYLKNETSKNALKEGKEIEY
                     EYEPVRLGPMPPETEWLFKKYKIPVPRAIRHVYGRVRDVMTRDIACVREDETVMLAGR
                     RMRKNKVKALVVIDRYGNYKGLITTRMIADRYISATDKINKNGTNMAVVAADLVTSLD
                     EKVKDLMETDVLVLDDEDLLKDAIPELLESPLREAVILDDDKKKCIGIITRSDVARRL
                     HKNVVLVDHNEASQAATGIKEANILEIVDHHRIADISTLSPIKFVNLPVGSTASIVTS
                     EFENYKHRKVPIPKPLAAALLSAIITDTIILNSPTTTAFDQRMAKDLAKIAGVDIQKF
                     GYQVFNQRGGDEKMEVKELLLADSKEFQLGDQRVLICQHETINVKRVMKREAEIRKFM
                     AKVRKERDYEFVLFLLTDIIKQGSLFIGSGDLDILNRLFDIECHGKGGTWMPGIVSRK
                     LQVAPKILGA"
     gene            7058..7540
                     /locus_tag="AC04VQ_02365"
     CDS             7058..7540
                     /locus_tag="AC04VQ_02365"
                     /inference="COORDINATES: protein motif:HMM:NF036577.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIO9553062.1"
                     /translation="MGSKKEYDLIEAIEAVASEVAPELDIVDVRIVGAAQAPIVRVYI
                     DHPKGVTFDVLTNTQKWISDLLDEVNPFTSAYTLEVSSPGPERPLRKLEHFQAAVGKK
                     AKIKCSEPIDNRKNFTGTIVSASKDIEIALDEGKPGAEPKVQIAFELIASANIVPGED
                     "
     gene            7569..8768
                     /gene="nusA"
                     /locus_tag="AC04VQ_02370"
     CDS             7569..8768
                     /gene="nusA"
                     /locus_tag="AC04VQ_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIO9553063.1"
                     /translation="MATNTLLDALQALAHEKRIDEVYLIERLEESLASSYKRTLKLEN
                     DCRVIIDRKAGEIYVFEKVPIGEPDPETGEYSEFDEVDVTPDDVSRLAAQNAKAVIRS
                     LVRDAARQSIYEEFADRVGDIVMGTVLQRTKDFTIIKIRDGVEAELPHFDTKRNPNER
                     NECPANEHYKHNQRLRVLIIDVRNPLDPNHTKGENSRPSIVVSRTHPNLIKALFENEV
                     PEIYDNLVEIKSVAREPGARSKIAVASNEPNLDPVGACVGPKGSRVRMVVEELHSERV
                     DVIQYEEDPARYIMNALSPARIMNVTLDEESKTATVLVSDDQLSLAIGKEGQNARLAA
                     RLTGWSIDIQSTSFTGEDLDLDDEVEVDDEFAGICEYENEEGVRCRNHARPGSKYCGI
                     HAKMAEK"
     gene            8811..11666
                     /gene="infB"
                     /locus_tag="AC04VQ_02375"
     CDS             8811..11666
                     /gene="infB"
                     /locus_tag="AC04VQ_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013979679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIO9553064.1"
                     /translation="MSIRVAQLAKELGLKPPELLDKLNEIGIPIKTQGMPLNDAQEKK
                     ARAELGKDAKKEEKKTAAKKTSAAAKKSDKPSKEDEAKKKRLEAVKAEQAARAAERAA
                     QTDSSLLGSNVDLIDQKKIDEERAKKSAEVAEAKPEKPAKEKEEVKKVTKQLKSIVEE
                     TAKRAKELESLSKQIKDDKERKKLEKEIAKNEKRMAEAQKAVEKQKAVEAALKAKKAG
                     KGAKKAKEAEEAPEPKEPKKVLSATFTDEKAKGKIDELVAQISSEKERLKDEGVSIPV
                     SSKKKKGKKRGKLEPDVPELEPVMSKEDRYAAMAAAAEKLQRDKVLEEARAAVAAATH
                     DGEGRRKKRKEKREAEAREKAELEAIEKGLDPDIVFDDSVAEIQEGASVGDFAEALNV
                     PPNDVIKRLFMLGQALTLTQTMSNDLIELIAADLERKIRIVSPEEEFNVVYNDTEKDL
                     KSRPPVVTVMGHVDHGKTSLLDAIRDTAVIESESGGITQHIGASVVFKNDRQIAFIDT
                     PGHEAFTAMRARGAEITDVIVLVVAADDGVMPQTKEAINHAKAADVPVVVAVNKIDKQ
                     GANPERVKQELTEYGIVPEEWGGKNMFVEISAKQKTNIDELLETILLQADVLELKANP
                     DALASGYVIEANLDKGRGPVATVLVNRGTLKEGDTIVAGTSMGKVRALIGPFGKRYKG
                     AEPGEPAEILGLDSVPVAGDDFRVFDDEREARKLVENRRLRARIASQNAAKVSLDNLF
                     DRIEEGKQTDLNLIIKADVQGSIEALRDALEKMDQEEVRINIVHSDVGGITETDVTLA
                     TASEAIIIGFNVRPTGKSKQLAEREQVDIRMYKVIYQAIEDINAARVGMLKPDIVEKD
                     TGIAEVRETFKVPKIGLIAGCYVVEGEISRDDSVRVVRDGTIVFDGKIASLKRFKDDA
                     KSVKEGYECGIGIDGFQDIKAGDTIEGYKTVEVERTE"
     gene            11783..12136
                     /gene="rbfA"
                     /locus_tag="AC04VQ_02380"
     CDS             11783..12136
                     /gene="rbfA"
                     /locus_tag="AC04VQ_02380"
                     /inference="COORDINATES: protein motif:HMM:NF014130.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIO9553065.1"
                     /translation="MPKEDYRFSPGARRANEQVREHVANILLFDISDPRLQMVTITGC
                     DVSPDKRNAKVYYTTDKEKYEEVAESFEKAKGYIRKLLGDRLEWRVVPAVNFILDPSV
                     DMGEKIAKAFGKENE"
     gene            12117..13037
                     /locus_tag="AC04VQ_02385"
     CDS             12117..13037
                     /locus_tag="AC04VQ_02385"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013528.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIO9553066.1"
                     /translation="MEKKMNSFEEIFDAIKKHDTFYLSGHKNPDGDSIGSCKCLQLAL
                     EQLGKKVQSMPGNNYIFFQLDVQPDYRLPDEALEAKQKAKVKICFDHHQPDEANCDLV
                     YVDPSAAANALIIWDFVKFMNVKITKEIATAAYYGLCSDTNSFMNTNSDQRAFEAATE
                     MMTYGVDAAKVAQELFQSRTLQSFELEKVALDHLFVDEDKHFAFSFLTAEDYAKYNAT
                     KADSDAIIDLLRELECVEVACVIRQEEPGQKVRGSLRSKGNIDVSKLARLHTGGGHKA
                     ASGFTMDETDIARACETVKSQLLDLMNGKI"
     gene            13047..13550
                     /gene="truB"
                     /locus_tag="AC04VQ_02390"
     CDS             13047..13550
                     /gene="truB"
                     /locus_tag="AC04VQ_02390"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIO9553067.1"
                     /translation="MKRGNTDLNFLIAINKPTGMTSHDVINRIRTITGEGRVGHMGTL
                     DPLASGVMIIGVGSAARLNNYLEEDEKTYVAKITLGKSTNTFDAEGEVTQSADVNQKF
                     LTENFVKDYLSNMVGLITQTPPAFSAIKINGKPAYKDARAGKDVDLPEREVEIYSANL
                     ISIEDDS"
     gene            13554..>14331
                     /locus_tag="AC04VQ_02395"
     CDS             13554..>14331
                     /locus_tag="AC04VQ_02395"
                     /inference="COORDINATES: protein motif:HMM:NF018299.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553068.1"
                     /translation="MEFEVSKGTYIRSIANSIGQDLGCGAYLQALERIASGLISINEA
                     YSLEDFENAFKSGTYKILNAVDMLTLPVVEADEKMLKRIKNGNSLRNNSEWNLFPGQN
                     VSIYDNHKLLAIYKLKDLHELICECKFSGAIDLPKNETYIKCPNGYFHIFVDDLHLSA
                     DYCATLGVFDGFHEGHAYEVEECIADAKARGCNSVAITFDVDPDEIFKKDFVKLDSNE
                     DRISKLKASGVDEVRVLNFGEIKDLEGEEFLEKYFGKNPPQ"
BASE COUNT         4080 a         2794 c         3640 g         3817 t
ORIGIN      
        1 tttcgctatg tagccgccag ggtgcaaaat tagtgcccct cgcgagtgcg accacggagg
       61 tggggatcac ccgatcccat tccgaactcg gtagttaagc ccaccatcgc cgaaggtact
      121 acattgtagt gatgtgggag aataggacgt cgtactcgca aggggctctt tttttatatt
      181 ttttgagtca atttttttat aatctgttat tctgtttatt tggtgaaagg agagaaaatg
      241 aaggatattg aaaagagaaa tattgctgct tgtgtagttt tttcaattat tacttgcgga
      301 atttatagtc tttactggat ttataagatg aatgaggaaa ttaacattat ttcaaagcac
      361 gaagatgaca caagtggtgg cctcgtaatt ttattcggaa ttattacttg cggaatttac
      421 tatgtttatt gggcttataa aatgggtgat aagctcaaag agtattacac caagaaaaag
      481 tctaacgaag gcaatgatct tcctgtgctt tatcttattt tgatgattgc gaattatgtt
      541 gcatgtggaa tttgtaccat tatttgttat tgcttaatgc aagacagaat taataaaatt
      601 gttacaaaaa agtaaaaaaa gccgattgca aaaattaaca attgccctca tagccgtatt
      661 gctgtgcggg cttttgtatt ttttgtttgt gtctttgaca gggattggaa ttccttgccc
      721 aattaagttt tgaacaggct ttaagtgccc gggctgcgga ataacaacca tgttcattga
      781 gctgtttcac ctaaatttcg aagcagcatt ttgagccaac cctctaactt tttgcctatt
      841 gccatttatt atcgcgctca taatttacct atgtgttgtt tatgtacgca agggttcgtt
      901 gcaacttcca aaatggttaa cggttgcttt aattgttctt attgttttat atgttctttt
      961 tggaattttc cgaaacatat ttttatttta ataacgaaga tctttatagg ggatgtgcaa
     1021 tcgcttcgat tctgaaagca agtctttgcc tgtaattccg tttgcttgga gaagagccat
     1081 catggtaagc gaatttttga aggaatcatt tgcaattggg tgaaactcca ttccttcggc
     1141 ggttcaaaaa gcgtaggctg ccacgccttc ggtgaaaaaa gtcgggacaa tgctatatga
     1201 tttaagccct gttccaggca tttttaagag gggccgagca atcagcaggc aagggtcaac
     1261 gcgaaagaca gtggcaagtt tttcgatgag gtcaatgccg gtagagattc ggcaattttc
     1321 gatattgccg atttgtgaaa tgttgatttt ggatttttca gagagcgttt cttgagataa
     1381 gttgaagtat tttcgatacg cataaacatt agcccccagt ctttgacgca acatttatac
     1441 tcctttaaaa ctacgtttat atttattgtt tcaggaatat taagtttgtc tacagattat
     1501 ttgttgcgtt ttagaagata gtggcaaagc aggaagacga cgaggctgac cgcgatgagg
     1561 atgccgaaga taacgaggta gccggcttcg gggttttgcg tggtggcgtc aagaattgcg
     1621 ccgatgccag tgttaagcca gatgtcgggg ctgaaaacaa tcatcgaagc gaggccggtt
     1681 gcggtgccag cgaccttcgc cgggagattg atttcggaga gaattgccca ttgaactgcg
     1741 taaatcatgg ttgagaaaat gcctggcaaa atggagatga ccgcaataag cacaatattg
     1801 tttggaatgc cgaagacggc gcgcgcatcg ctgaacccta ctagaataaa aattgcggtt
     1861 gagatggcca tgcaaattgt ggcaacgcga taccacttta gcgtgctgtg gaaaatttta
     1921 tccgcgatca tgccgctgaa aattgcggca aaaatcatta tgaaatacgt gcggaaaatt
     1981 gtaagcgcgc cactaatttc gtcgctcatt ccgcaaaccg cagtcatgta ggtcgtaaaa
     2041 tacgtggtca gcgtaaagat aatgtaaacc agcataattg tgatgccagc aattcaaacg
     2101 gtgccatttt taagtacggc cttaatgtct tcgcgcttgt aagactcgtc gcttttcggc
     2161 atttgtaaat ttttctcctt gaaaagaatg gtgatggcca gcatcgcaac gccgatgaaa
     2221 aacgcaacag aaattgtgac gcccataatt ccattgcctt cgcctgaaaa ttgcgtgtac
     2281 ataaacatat tgctgctgga tacgatcatg ctaaagatcg cgttcgccgc aaagtaaatg
     2341 ccgaagcagc ggccttggtc gtcttcgcca cctgtgagac gaatcgcttt aaccagcgat
     2401 gaccaaaaaa cgaaagtggt ggcaattggc attaggcctc aaacaccgag cgcaatccaa
     2461 tatggggttt tatctccgag gtgaagccca aggacaatcg caaatccaat cagcaaaacc
     2521 agggcccatc ccatggacat taaaagtggc tttttagcgc gaagtttgtc cgccaaaata
     2581 ccgccaggaa tgtagaggat gatcgaaaca atgccgtaca ctgtcagcaa aagtccagct
     2641 tgagtatcgt tgattccaag cgtttcattc agcggatttt gaaaaacata acgaatgtat
     2701 gggtagatat acataacgcc acccgcaaga ccaagtgcaa acagcgtcaa atatttcttc
     2761 aaattcctca cccttttatt ataaatgaat ttttgatata atagaccagt tatgagtaag
     2821 aagataaaaa atttaggaat tattggcggg acctttgatc caatccatta tggtcatatt
     2881 ggttgtgccg aagccgccgc caacgccttc gaccttgacg aagtttggtt tatgccggcc
     2941 aatcatccta attttaagca ggataaaaac gtcaacgaca tcaaggatcg tttgaagatg
     3001 tgcgaaatgg cgcttcgtgc ttatggaaat gagaagttta aggtttctga tctcgaggct
     3061 aatcgtgacg gcgtgacctt tacttcggag actttggaaa ttttaaagaa cgattatcct
     3121 gataccaaca ttttctttat caccggcacc gatgcggttt tcgcacttcc aagctggcat
     3181 aactacgaag agatccttga gctcgccaca attatcgcgg ttactcgcaa gggttatcac
     3241 gagccaaatg ccgaacagga aaaatttctc gagaaatacg ccgacaagat taagctggtt
     3301 gaggctgatg ttattgacat ttcttcaagc caaattcgtc agcgcctctg gaacaggcaa
     3361 gacgttagcg attacgtgcc atcgtcaatt ttcaattaca tgctcaacaa caacctctat
     3421 taaaagatgc atttcatgcc tggcaagaat tacacctttt ggtgcatttc atgcctggca
     3481 agaattacac cttttaattt tagattaagg ttctttttat agaattcgct tgtcgaaaaa
     3541 aggagattat atgaaaagat gagtgaaaaa tttaatttgt tttgggggca ttggattgat
     3601 gttggttgcg atttttgcga ccatgtattt tggtaaggca cttgccccag gtggagatat
     3661 gcaaggtggt caaggcggtc ctggcgaagc tatggagcaa cctcaagatc aaggcgggac
     3721 tcctcctaca aaacctgatg gagattcgca atcgcaaggt tctacaaccc agccaccaag
     3781 tattcctgaa ggaggcggtt atgcaggaag ttctagcaag ggtgacagtg agcttccgca
     3841 gaattcgcaa agtacacctc ctgcgaagcc agaaggtgaa agcaattcgc agcaaggagg
     3901 gcaaggtgga actccgccag atatgccaag tggtggaatg caacaaggtg gaggcatgca
     3961 acaaggtgaa atgccacaag gtgaaatccc acaaggtaat agcgaggaaa tcggcgaagc
     4021 cggctggacc aagattgcac tttatggtgc ggagagttta gtcattgcgt tgcttgttgc
     4081 ttacttaatt atttcgcaat gtaacaagcg taagtttaag gaatctttta aggcaggaaa
     4141 atcgaaggct gtgttcatcg tagtaacagt tgccattaca gcagttggag gctgtggtct
     4201 tgcctacgtc tcgaccgaga ttgatggttt ggccgcgcct caaaccggtc aaagttcttc
     4261 ggcggaatat aaggccgtta aaactataag tgaaaatact aatttggatg gaggctctta
     4321 tgagagcact gaatccgata ctttagcagt gcttgtaatg aacaaaatta atgcgacgct
     4381 taaaaatttc gagcttacaa aaactggttc ttccaacgga ggtgataaca ccagcttcta
     4441 tggcacaaat tcaggtatta ccgcaaagga ttgcgcgaac ctaataattg aaggtgcaaa
     4501 aattttgaca gatgcaagtg gtgcaaacgg agtttttagt tttggaggca ctgctgttag
     4561 tggcgttcaa actggcactg gggatggcac cgaagtaacc gttcgtgatt cggaaattac
     4621 cactaagcag gacaattccg gcggtgtcat gctaactggc ggcggtattt taaatgtcat
     4681 caattgcgtc attgaaactt ttggaacgtc tagtgcggcg cttcgaagcg atcgaggcgg
     4741 tggcgacttt aacgtggaag gcggaactta caaaacttga ggcaacggtt cgcctgcaat
     4801 ttattgcacc gctgacatca atgttaagga tgcggatttg caatcctatg cttcggaggg
     4861 cgtggtcatc gaaggtaaaa atagcgttaa cctggaaggc tgccacctat ttgatcaaaa
     4921 ttcgaagctg aatggccagt ccacaacata taaaaatatt ttcctctatc aaagtatgag
     4981 tggcgatagc gcggaaggca catcgaagtt cagcgcaaag ggcagcacgc ttgagacttc
     5041 caagggtgac actttctatg taaccaacac gaagagtgtg atcacggttg aagattgcca
     5101 ggtcattaat aatgacacga gcggaaacct ccttcgtgcg aaagcagata gctggggtaa
     5161 tagcggtcaa aacggtggtg atgttgaatt taacgtgacg aagcaaagtc tgaccggcaa
     5221 catcgttatt gatgcaattt cgaagctggc cttcggctta actaatggaa gttattggga
     5281 aggcaccctc aacggtgaca atgcttcgca aaatataagc ctcaagctgg acagatcttc
     5341 gaagattaaa cttacgggag atagctatgt aacaagtctt gatgacgaag ataccagcta
     5401 cagcaacatc gactttaatg gtcacaaact ttacgtaaac ggcaccgcca tcaattaaaa
     5461 ggtgtaattc ttgcctggca atttttacac cttttggtgc atttcatgcc tggcatggat
     5521 tgcacctttt ttgtggtata atgtcatttt ttggaaggag gcgatatgcc taagaaagtt
     5581 tatgtaatgg gacacaagaa cccggacaat gattcgattt gttcggcggt tgcttatgcc
     5641 tatttgaaaa atgagacttc gaagaatgcg ttgaaggaag gtaaggaaat tgagtatgaa
     5701 tatgagcctg ttcgcttagg gccaatgcct cctgagactg aatggctttt caagaagtat
     5761 aaaatcccag ttccgagggc aattcgccac gtgtatggtc gtgttcgcga cgttatgact
     5821 cgtgatattg cttgtgttcg tgaagatgaa accgttatgc ttgcgggacg tcgcatgcgc
     5881 aaaaataagg ttaaggcgct tgtagtaatt gatcgctatg gaaattacaa gggccttatc
     5941 actacgcgaa tgattgctga ccgctacatt tcggcgactg ataaaattaa taaaaatggg
     6001 acaaatatgg ctgttgttgc agcggatttg gtaacttcgt tagatgaaaa agtcaaggat
     6061 ttgatggaga ccgatgtttt ggtcctcgat gacgaagact tgctcaagga cgcgatccct
     6121 gaacttttgg agagtccgct tcgtgaagct gtaattttag atgacgacaa aaagaaatgc
     6181 attggtatta ttaccaggtc tgatgttgct cgtcgtttgc ataaaaatgt tgttttggtt
     6241 gatcataacg aagcctcgca ggccgcaact ggcattaagg aagcaaatat tcttgagatt
     6301 gtcgaccatc acaggattgc tgatatttcg acattaagcc caattaagtt tgtgaacttg
     6361 ccggttgggt cgaccgcttc gattgtcacg tcggagtttg aaaactacaa gcacaggaag
     6421 gttccaatcc cgaagccttt ggccgcggcg cttctaagtg cgattattac agatactatc
     6481 attttgaatt cgccgacaac taccgctttt gaccagcgta tggcgaagga cctcgcaaag
     6541 attgcgggtg ttgacattca aaaatttggc tatcaagttt tcaaccagcg tggtggcgat
     6601 gagaagatgg aagttaaaga gttacttttg gctgatagca aggaattcca acttggtgat
     6661 cagcgcgttt tgatttgcca gcatgaaact attaatgtta agcgagtgat gaagcgcgag
     6721 gctgagattc gcaagttcat ggcgaaggtt cgcaaagagc gcgactacga atttgtgctt
     6781 ttcttgctga ctgatattat caagcagggt agtttgttta ttggtagtgg tgatttagac
     6841 atcctcaaca ggctttttga tatcgagtgt cacggcaagg gcggcacttg gatgcctgga
     6901 attgtttcgc gcaagcttca agttgcgccg aagattcttg gggcataaaa aacttgcaat
     6961 ttagagcgta agtgctataa tacccgctta caaagaaagt taaaacgaag agacggtcac
     7021 tggccgcctc tttttttaca ctttttggga aaggaaaatg ggtagtaaaa aagaatatga
     7081 cttgatcgag gctattgagg ccgttgcttc ggaggttgct cctgagcttg acattgttga
     7141 tgtcaggatt gttggggctg ctcaagctcc gatagtgcga gtctacattg accatcctaa
     7201 aggcgtaact tttgatgtgc ttactaatac ccaaaaatgg attagtgact tgcttgatga
     7261 ggtcaaccca tttacgagcg catatacgct ggaagtttca agtccggggc cggagcggcc
     7321 gcttcgtaag cttgagcatt ttcaagcagc ggttggcaag aaagcgaaga tcaaatgtag
     7381 cgagcccata gacaacagaa agaattttac tggaacaatt gttagtgcca gtaaagatat
     7441 tgaaattgca ttagacgaag gtaagcctgg agctgaacca aaggtgcaaa ttgcatttga
     7501 gcttattgca tctgcgaata ttgttcctgg tgaagattga taaaaaagtt tattaaagga
     7561 gttaaatgat ggctacaaac accttattag acgccctgca agcgttggct cacgaaaagc
     7621 gaatcgatga ggtttactta atcgagcgtt tggaggagag cttggcttca agctacaagc
     7681 gtacgcttaa gcttgaaaat gactgtcgag taattatcga ccgtaaagca ggggaaatct
     7741 atgtattcga aaaggttcca attggcgaac ctgatccaga aactggcgaa tacagcgaat
     7801 ttgacgaagt tgacgtaacg ccggatgacg tttcgcgcct cgctgctcaa aatgcgaagg
     7861 cggttatccg aagccttgtt cgcgacgctg cccgtcaaag tatttacgaa gaattcgcag
     7921 accgcgttgg cgacatcgta atgggaacag ttcttcagcg cacgaaagat tttacaatca
     7981 tcaagattcg tgatggcgtc gaagctgagc ttcctcactt cgatacgaag cgcaatccta
     8041 acgagcggaa cgaatgtcct gctaacgagc actacaagca caatcagcgc ttgcgcgttt
     8101 tgatcattga tgttcgcaac ccgcttgacc cgaaccacac aaagggtgaa aattcgcgtc
     8161 caagcattgt tgtttcgcgc acgcatccaa atttgatcaa ggcacttttt gaaaatgagg
     8221 ttcctgaaat ttatgacaac cttgttgaga ttaaaagtgt tgcacgcgaa cctggcgctc
     8281 gttcaaaaat tgcggttgct tctaatgagc caaatcttga ccctgttgga gcatgtgttg
     8341 gacctaaggg tagccgtgtt cgaatggttg ttgaagagct tcacagcgag cgagttgatg
     8401 ttattcaata cgaagaagac ccagctcgct acatcatgaa cgctctaagt cctgcgcgta
     8461 tcatgaatgt tacgcttgac gaagaaagca agactgcaac cgttttggtt agcgatgatc
     8521 aactttcgct tgcaattggt aaggaaggtc aaaatgctcg tttggctgca cgcctcactg
     8581 gctggagcat cgacattcaa agtacttcgt ttacaggcga agaccttgac ctcgatgatg
     8641 aagtagaagt agacgatgaa ttcgcaggaa tttgtgaata tgaaaacgaa gaaggcgtcc
     8701 gctgcagaaa tcatgctcga cctggttcta aatattgtgg aattcacgcg aaaatggcgg
     8761 agaagtagtt cggagatttt gggaaggaaa ggaagaggtt aggagataac atgtcaatac
     8821 gtgtagcaca attggcaaaa gaactcggct tgaagcctcc tgagctgctt gataagttga
     8881 atgagattgg gattccaatc aagactcaag gtatgccgct aaatgatgcg caggaaaaaa
     8941 aggcccgcgc tgagcttggc aaagacgcta aaaaggaaga gaaaaagacc gctgcgaaga
     9001 agacttcggc cgcggcgaaa aagtccgata agccatctaa agaggacgaa gccaagaaga
     9061 agcgtcttga agctgttaag gctgagcagg cggctcgtgc tgcggaaaga gctgcgcaaa
     9121 ccgattcgag tcttttaggt tctaatgtcg atttgatcga tcagaaaaag attgacgaag
     9181 agcgcgcgaa gaagagtgcc gaagttgctg aagccaagcc tgaaaaacct gctaaggaaa
     9241 aggaagaggt taaaaaggtt accaagcaac ttaagagcat cgtcgaagaa actgcgaagc
     9301 gcgccaaaga gcttgaaagt ttgtctaagc aaattaagga cgataaggag cgcaagaagc
     9361 tcgagaaaga aattgccaaa aatgaaaagc gtatggccga agcgcaaaag gctgttgaaa
     9421 agcaaaaggc tgttgaggct gcgctgaagg ctaaaaaagc tggtaagggt gctaaaaagg
     9481 ctaaggaggc cgaagaagcg cccgagccaa aagagccaaa gaaggttttg tctgcaacct
     9541 ttactgatga aaaggcgaag ggcaagattg acgaattggt tgctcaaatt tcgagcgaga
     9601 aagagcgttt gaaggacgaa ggcgtttcga ttcctgttag ctctaagaag aaaaagggta
     9661 agaagcgtgg caagcttgag ccagatgttc cagagcttga gccagtcatg agcaaggaag
     9721 accgttatgc tgcgatggcg gctgcggctg aaaaacttca acgcgataag gttttggaag
     9781 aggctcgtgc ggctgttgcg gctgcaactc acgacggtga gggccgtcgt aaaaagcgta
     9841 aagaaaagcg tgaagccgag gctcgcgaaa aggctgagct cgaggcaatc gagaagggcc
     9901 ttgatccaga catcgttttt gatgacagcg ttgctgaaat tcaagaaggt gcaagtgttg
     9961 gtgacttcgc cgaggcgcta aacgttcctc caaatgatgt tatcaagcga cttttcatgc
    10021 ttggccaggc gcttacgctc actcaaacta tgagcaacga tttgatcgag ctcattgcgg
    10081 ctgacttgga gcgcaagatt cgcatcgtta gccccgaaga agaattcaac gttgtttaca
    10141 atgatactga aaaagatttg aaatcgcgcc cgccagttgt tactgttatg ggtcacgttg
    10201 atcatggtaa gacttcgctg ctcgacgcca tccgtgacac tgcggtaatt gaatctgaaa
    10261 gcggtggaat tacgcagcac atcggtgctt cggtagtttt taagaatgac cgtcaaattg
    10321 cgtttatcga tacacctggt cacgaggcct ttacggcgat gcgtgcccgc ggtgctgaga
    10381 ttaccgatgt tattgttctt gttgttgctg ccgatgacgg cgttatgccg caaaccaagg
    10441 aggccattaa ccatgctaag gccgccgatg ttccggttgt tgttgctgtg aacaaaattg
    10501 ataagcaggg tgccaaccct gaacgtgtca agcaggagct gactgaatat ggcattgtcc
    10561 ctgaagagtg gggcggcaaa aatatgttcg tcgaaatttc ggcgaagcaa aagaccaaca
    10621 ttgacgaact cctcgagacc attttgcttc aagcggatgt tcttgagttg aaggccaacc
    10681 cggatgcctt ggcttcgggc tatgtaattg aggccaacct cgataagggg cgcggtccgg
    10741 ttgcaactgt tttggttaac cgcggaacct tgaaggaagg tgacacaatt gttgctggta
    10801 cttcgatggg taaagttcgc gcgctgatcg gtccgtttgg caagcgctat aagggtgccg
    10861 agcctggcga gcctgccgag atcctcggtc ttgacagcgt tccggttgcc ggtgatgatt
    10921 tccgcgtttt tgatgatgag cgtgaggcgc gtaaacttgt ggagaaccgc aggcttcgtg
    10981 ctcgcattgc ttcgcaaaat gcggcgaagg tcagcctcga caatttgttt gaccgcatcg
    11041 aggaaggcaa gcagaccgac ctcaacctca ttattaaggc tgatgttcaa ggttcgatcg
    11101 aggcgcttcg tgatgcgctt gagaagatgg accaagagga ggttcgcatc aacattgttc
    11161 acagtgatgt tggtggtatt accgaaactg atgttacgct tgcgacggct tcggaggcta
    11221 ttattattgg atttaatgtt cgtccgaccg gtaagtctaa gcagttggcc gagcgcgagc
    11281 aggttgatat tcgcatgtat aaggtcattt atcaggctat tgaggatatt aatgctgcgc
    11341 gtgttggcat gcttaagcct gacattgttg agaaggacac tggtattgcc gaggtccgcg
    11401 agactttcaa ggtgccgaag attgggctta ttgctggctg ctatgttgtc gaaggtgaga
    11461 tttcgcgcga tgatagcgtt cgtgttgttc gcgatggcac tattgttttc gatggtaaaa
    11521 ttgccagctt gaagcgcttt aaggatgatg cgaagtccgt caaagaaggc tatgaatgcg
    11581 gtattggcat tgatggtttc caagatatta aagccggcga caccatcgaa ggctacaaaa
    11641 ccgtcgaagt ggagcgcacc gaataaagtt ctccccgggg taggagaaga gatttaaaag
    11701 tggtatctcc ccggggtcca atactcgatt ttcaaagaaa atcgttagta gtggccccgg
    11761 ggtagacata aaggaaagaa gaatgccgaa agaagattac agattttcgc cgggagctcg
    11821 aagagcgaat gagcaagttc gtgagcacgt cgcgaatatt ttgcttttcg atatttcgga
    11881 cccgcgcttg caaatggtga cgattaccgg ttgcgatgtg tctccggaca agcgcaacgc
    11941 gaaggtctat tacacgactg ataaggaaaa atacgaagag gttgccgaga gtttcgagaa
    12001 agcgaagggt tatattcgca agcttttagg tgaccgcttg gaatggcgag ttgttccggc
    12061 tgtgaatttt atcctggacc caagtgttga catgggcgag aaaattgcca aggcttttgg
    12121 aaaagaaaat gaatagcttc gaagaaatat ttgacgcgat aaaaaagcat gacacttttt
    12181 atttgagtgg ccataagaat ccggatggcg atagcatcgg atcttgcaaa tgtcttcaac
    12241 ttgcactaga gcaacttggc aaaaaggtgc aatccatgcc tggcaataat tacatctttt
    12301 ttcagctgga tgttcagcct gattatcgac ttccggatga ggcgcttgag gctaagcaaa
    12361 aagcgaaggt gaaaatctgc tttgaccatc atcagccaga cgaggctaat tgcgacctcg
    12421 tttacgttga cccaagtgct gcggctaatg ccctgattat ctgggacttc gtcaagttta
    12481 tgaacgtcaa aattacgaag gagattgcca ctgcggctta ctatggcctt tgcagtgaca
    12541 cgaattcgtt catgaatacc aactctgacc agcgtgcttt tgaggccgcg accgagatga
    12601 tgacttacgg cgtcgatgcc gcgaaggttg cccaggagct ttttcaaagt cgcacgcttc
    12661 aaagtttcga gcttgagaag gtggcgcttg accatttatt tgttgacgaa gacaagcact
    12721 tcgcttttag ttttttgacc gccgaagatt acgccaaata taacgctacc aaggcggaca
    12781 gtgatgcgat catcgatttg cttcgcgagc ttgagtgcgt tgaagttgct tgtgtgattc
    12841 gtcaagaaga gcctgggcaa aaggtccgcg ggagcttgcg ttcgaaaggc aacatcgatg
    12901 tttcgaagtt agcgaggctg cacacaggtg gcggtcacaa ggcggcttcg gggtttacga
    12961 tggatgaaac cgacatcgct cgcgcgtgtg agactgtcaa gtcgcagctg ctcgacctga
    13021 tgaacggcaa aatttaggcg gccactatga agcgcggcaa caccgatctt aattttctaa
    13081 ttgcaattaa caaaccaact ggtatgactt cgcacgatgt tatcaatcgc atccgcacga
    13141 ttaccggcga aggccgcgtc ggccacatgg gcacgctcga cccgctggct tctggcgtca
    13201 tgattattgg tgttggttcc gctgcacgcc ttaataacta tttagaagag gatgaaaaaa
    13261 cttatgttgc aaaaattact ttaggtaaat ctacaaatac atttgatgct gaaggtgaag
    13321 ttactcaatc agctgatgtt aatcagaaat ttttaacaga aaattttgta aaagattatc
    13381 tcagtaatat ggttgggctt attactcaaa ctcctccagc tttttctgct attaaaatta
    13441 atggaaaacc tgcttataag gatgctcgtg caggtaagga tgtggatctt ccagagcgcg
    13501 aagttgaaat atattccgca aatttaataa gcattgaaga tgatagttga aatgtggagt
    13561 ttgaagtttc aaagggcacc tatattagaa gtatcgcaaa ttccattggt caagatttgg
    13621 gttgtggtgc atacttacaa gctttggagc gtatagcttc tggtttaata tctattaacg
    13681 aagcctacag tttagaagac tttgaaaatg ctttcaaatc tggtacttat aaaatcctca
    13741 atgcagttga tatgttgaca ttacctgttg ttgaagcaga tgagaaaatg ttaaagcgca
    13801 ttaagaatgg aaattcgttg cgcaataatt ctgagtggaa tttatttccg gggcaaaatg
    13861 tttcaattta tgataatcat aaattgttgg ctatatataa gttgaaagat ttgcatgaat
    13921 taatttgtga atgtaaattt agtggtgcaa tagatttgcc aaaaaacgaa acctatataa
    13981 aatgccccaa tggttatttc catatatttg tcgatgactt gcatctttca gctgactatt
    14041 gtgcgacgtt gggtgtcttc gatggttttc acgaagggca cgcttacgaa gttgaagagt
    14101 gcattgccga tgcgaaggct cgcggttgca attctgttgc gataactttt gatgttgatc
    14161 cggacgaaat tttcaagaaa gattttgtga agcttgacag caacgaagat cgcatctcaa
    14221 aacttaaagc aagtggagtt gatgaagttc gcgtcctaaa cttcggagag atcaaggatt
    14281 tagaaggcga agaattttta gaaaagtatt ttggtaaaaa cccacctcag a
//
LOCUS       DBNCDC010000018        13662 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_1573_length_13662_cov_6.930550, whole genome shotgun sequence.
ACCESSION   DBNCDC010000018 DBNCDC010000000
VERSION     DBNCDC010000018.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 13662)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13662)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13662
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1573_length_13662_cov_6.930550"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            147..443
                     /locus_tag="AC04VQ_02400"
     CDS             147..443
                     /locus_tag="AC04VQ_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553069.1"
                     /translation="MNLIKKSDDKLTLDGKRSFLFLCLGILAICMIALFGCKSQTTNE
                     TIDKTNLPPEKDSNSLNVEFSGEHVTADVPQTVQKGNDLTLSLDIEYFRDPNTT"
     gene            585..896
                     /locus_tag="AC04VQ_02405"
     CDS             585..896
                     /locus_tag="AC04VQ_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553070.1"
                     /translation="MYQRNNINTLKIKKEYVVNDIKIQVVGTPRTATLTLYGIEFDDD
                     FAAQIKNGTVKATFKTAYQNYGTVPIRTFSNGELNCVFEDDDIDVTFTTTDPNGLPKN
                     L"
     gene            1036..1302
                     /locus_tag="AC04VQ_02410"
     CDS             1036..1302
                     /locus_tag="AC04VQ_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553071.1"
                     /translation="MKKIIAVISTGVFLFIFAICLNSCVGNVDLSKVKDAYTQDILEN
                     TDISPYDTKSVEDIKKELAVKVVDGIETCYFTNIDYKTDNNRAE"
     gene            1456..2274
                     /locus_tag="AC04VQ_02415"
                     /pseudo
     CDS             1456..2274
                     /locus_tag="AC04VQ_02415"
                     /inference="COORDINATES: protein motif:HMM:NF019735.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2392..2763
                     /locus_tag="AC04VQ_02420"
     CDS             2392..2763
                     /locus_tag="AC04VQ_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553072.1"
                     /translation="MLPAPDENGNIVYGGGFSEENNIACLMQKSVSHKENKFTVTCFG
                     CEDGMVIVGSDLEYTGEVTGPGKFFTTPRTIHTTLEQCNYKGSHKLSEDGKELTHGNV
                     VYKILDNDNKMQVSTQEITGS"
     gene            2821..3048
                     /locus_tag="AC04VQ_02425"
     CDS             2821..3048
                     /locus_tag="AC04VQ_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553073.1"
                     /translation="MAFIEHGSNKAKGYAYSIQPKSREDKKFELIDTGDSNIHAVKLP
                     NGKVVAAFYKNGSFDYNGNKYEGSIGEYKIF"
     gene            complement(3113..3247)
                     /locus_tag="AC04VQ_02430"
     CDS             complement(3113..3247)
                     /locus_tag="AC04VQ_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553074.1"
                     /translation="MLLFIFWFPINKRRLEEIQDEKEELLASIEKEKQDALKKLGLAK
                     "
     gene            complement(3323..3487)
                     /locus_tag="AC04VQ_02435"
     CDS             complement(3323..3487)
                     /locus_tag="AC04VQ_02435"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9553075.1"
                     /translation="MVADAVDDMEVKHGVREDGTSLSLMLFFRKVAQCLSFVAVNLSL
                     IAMGRYVVTN"
     gene            complement(4100..4609)
                     /locus_tag="AC04VQ_02440"
                     /pseudo
     CDS             complement(4100..4609)
                     /locus_tag="AC04VQ_02440"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(4618..5142)
                     /locus_tag="AC04VQ_02445"
     CDS             complement(4618..5142)
                     /locus_tag="AC04VQ_02445"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIO9553076.1"
                     /translation="MPESVNGFLGFGQWHHIEADDEITAFLKYKNGATGIFITTTGEY
                     PGCNRLEISGTRGKLTVEGDKLLFAKLDADTEEFSRNAEGPYDRPNVEEIEVETDGEN
                     PQHKGIIQNFTNALLGKEELKIHGNEGLDAVELINSIIYSGWHNGETIQVPVNEDDYY
                     NELQNKIKNSTFLN"
     gene            complement(5281..5748)
                     /locus_tag="AC04VQ_02450"
     CDS             complement(5281..5748)
                     /locus_tag="AC04VQ_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIO9553077.1"
                     /translation="MSTIKYGIIGFGAHAELCYAEMLLKNKEVENATIVAICEPNEKR
                     IEAVKPALPQDISYFTDYKEMLDSELIDAAIITSPHPFHPEMVIECLKRNISVMCEKP
                     AGVYAKQVKEMIEASKNSEALFGMMFNQRTNCVYRKAKEMIENEEIGKLQRIN"
     gene            complement(5758..6615)
                     /locus_tag="AC04VQ_02455"
     CDS             complement(5758..6615)
                     /locus_tag="AC04VQ_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIO9553078.1"
                     /translation="MIRLCAPTDEAGKDFDEAVFLMNKNGIHLAELRGIFDKSVLYIS
                     NDEANYCKQKLAENNIKVWSISSPIGKRDLFIPITEFEANIDRAIEKAKFFECENIRV
                     FSFFDSLDKIDEVLKRLNLCVEKASKFNLNVCLENEKGSYADTAKRTIELLDKIPNLK
                     LVYDSSNFIQVGEPSEMTLKEVFPRAYFVHFKDGIHVNDDADITPVGKGEANIAKLID
                     LCDKDMVFSLEHHLKFATADQPFEQIKDSCEFVYKNVGEAFVDSVKNAKIMLSQAGYT
                     EADEGNFIK"
     gene            complement(6708..7109)
                     /locus_tag="AC04VQ_02460"
     CDS             complement(6708..7109)
                     /locus_tag="AC04VQ_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553079.1"
                     /translation="MEDKRKPISASKFKRVSNNYLLKHQNFFKEIKSKTSNVNSKNKS
                     IAKNMSMYAGAVKNFDTMKIILKENLNLIKNFNAELSIKSKSQLIQAYKLQDVALCSS
                     AVLFSMIESGSKKFKTKILQIKFNNSKFIAK"
     gene            complement(7236..7427)
                     /locus_tag="AC04VQ_02465"
     CDS             complement(7236..7427)
                     /locus_tag="AC04VQ_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553080.1"
                     /translation="MSKEAYTYLANTNALEDTPIKRLTKLNKPAIELYKSKGVNLARE
                     YLQIALCSQHLNGGISVDK"
     gene            complement(7710..8438)
                     /locus_tag="AC04VQ_02470"
     CDS             complement(7710..8438)
                     /locus_tag="AC04VQ_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIO9553081.1"
                     /translation="MTTKTFTYDAVVIGSGAAGFNAANAILKDGRKSVALITEGLNFG
                     TSRNTGSDKQTYYKLSLSGNSLDSVKLMAKNIFDGGAVDGDNALCEAALSTRCFLNLC
                     ELGVPFPTNRYGEYIGYKTDHDPFERATSAGPLTSKFMTEALQKQAFELDLKIHDKLY
                     AVEIIKDANKVAGLLCLDVRTNEYVLFNTPNVILATGGPAAIYDDNVYPKSQHGSTSI
                     AIKAGAYLQNLSEWQYGLASLKPK"
     gene            complement(8553..9176)
                     /locus_tag="AC04VQ_02475"
     CDS             complement(8553..9176)
                     /locus_tag="AC04VQ_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIO9553082.1"
                     /translation="MLEKVAIVTGSYRGIGKGIADMLSENGYTVVYSARHDSMEGVDY
                     IKCDISNPEDRNKLRDYVDKKYGRVDLLVNNAGVACKNRLDILETTEESFDRLIDINT
                     KGNFFMCQIFANYMIEKLKDNLEDYQPRIVNISSNSSYTSSVSRGEYCVSKAGLSMVT
                     KLFADKLGEYNIPVIEIRPGIIKTDMTEVVTEKYDKLIEEGITPMKR"
     gene            complement(9170..10192)
                     /locus_tag="AC04VQ_02480"
                     /pseudo
     CDS             complement(9170..10192)
                     /locus_tag="AC04VQ_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012927.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            complement(10211..10369)
                     /locus_tag="AC04VQ_02485"
     CDS             complement(10211..10369)
                     /locus_tag="AC04VQ_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553083.1"
                     /translation="MINEKINIEDKICANRVVAQPMEGCDCNLDGSPSELTIKKYINN
                     ANNGAGII"
     gene            complement(10375..10602)
                     /locus_tag="AC04VQ_02490"
     CDS             complement(10375..10602)
                     /locus_tag="AC04VQ_02490"
                     /inference="COORDINATES: protein motif:HMM:NF036773.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HIO9553084.1"
                     /translation="MVLETFKKANKDKSYILRGFECQGKKQRVNLNTKFNFKEAYICD
                     LLENEQRKLRVQNNKLEFEVKPFEIFTIKLK"
     gene            complement(10798..11820)
                     /locus_tag="AC04VQ_02495"
                     /pseudo
     CDS             complement(10798..11820)
                     /locus_tag="AC04VQ_02495"
                     /inference="COORDINATES: protein motif:HMM:NF019368.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
     gene            complement(11875..12084)
                     /locus_tag="AC04VQ_02500"
                     /pseudo
     CDS             complement(11875..12084)
                     /locus_tag="AC04VQ_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013256.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12274..12666)
                     /locus_tag="AC04VQ_02505"
                     /pseudo
     CDS             complement(12274..12666)
                     /locus_tag="AC04VQ_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013256.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 N-terminal
                     domain-containing protein"
     gene            complement(12670..13212)
                     /locus_tag="AC04VQ_02510"
     CDS             complement(12670..13212)
                     /locus_tag="AC04VQ_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553085.1"
                     /translation="MDLPTSLAKTAIRRDGKLYINGNRELFTNVEDFLTPQGMLYLNG
                     KLIQAIDNNHFEVKISKNGKYEMYMYLYTDVFEKAYPIDFSLKYKDERIEQAYYDFLC
                     VLENLDIITPTNKQYPKLIKIVNEAINMVDLRNKDEKFFNSLAKAKKFIKNKYFIEQG
                     GKENYFINCVGHSHIDMAWL"
     gene            complement(13270..13467)
                     /locus_tag="AC04VQ_02515"
     CDS             complement(13270..13467)
                     /locus_tag="AC04VQ_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553086.1"
                     /translation="MQYKIEEKLEAFVRSLNQFKTTEVISNLNISYAECKNPILENAK
                     KLKAYKKFKQPRLIYKSDAYY"
BASE COUNT         4317 a         2414 c         2144 g         4787 t
ORIGIN      
        1 ttcaagataa aacctacgta aaaaccatcg gctttaaccg ctcccactac gaaatataat
       61 tagctcctaa attgttattt actttataga aatgttataa tatgccttta taccgtatgt
      121 tagtttctag ttgggggagg tatgttttga atctaattaa aaaatctgac gacaaattaa
      181 cactagatgg taagcgaagt tttttgtttt tgtgtttggg aattcttgca atttgcatga
      241 ttgcactttt tggttgtaag tctcaaacaa ctaatgaaac aattgataag actaacttac
      301 caccagaaaa ggacagcaat tcacttaatg ttgaatttag tggagagcat gttacggcgg
      361 atgttcccca aactgttcaa aaaggtaatg atcttacttt aagcttggat attgaatatt
      421 ttagagatcc aaacactacc tgaaaaaata ttcctgatcc tcaaaagact ccaagtttat
      481 atcctgaagg agaacttact gttccaatta ctgaatatga agttttaatg gataatattc
      541 atgtttatat tggaggcaag gaatacaatg attcttatac ttgaatgtat caaagaaaca
      601 acataaatac attaaagatc aagaaggaat atgttgttaa tgatattaaa attcaagtag
      661 ttggcacacc acgaactgcc acgcttacgc tttatggaat tgaattcgac gatgattttg
      721 ctgcgcaaat taagaatgga acagttaaag caacatttaa aactgcatat caaaattacg
      781 gtacagttcc aattaggact ttttccaatg gtgagttaaa ctgtgtcttc gaagatgacg
      841 acatcgatgt tacatttacc actacagatc caaatggcct tccaaaaaat ttatgattta
      901 gaaattgttc gaggaacaca acattaggag aagatttctt cagagaatat tccgataatg
      961 gtaaaacttg ccatatctat gtgccacatt atttggtacg tgatcatagt tcaataagaa
     1021 atttggagga ttaatatgaa aaagataatt gctgtaatct ctacaggtgt tttcttattt
     1081 atatttgcta tttgcttaaa ttcatgtgtt ggaaatgttg atttaagcaa agttaaagat
     1141 gcatacactc aagacatttt agagaacaca gatatttcac catacgatac taagtccgtt
     1201 gaagatatca aaaaggaact tgctgttaaa gtagttgatg gtattgagac ttgttatttt
     1261 actaatattg attacaaaac agacaacaac agagcagaat gaaaggctaa taatcataat
     1321 gcgaatacgc taaaaatcgc tattgctgca ataaagtcag gtaatgatga acttaaagac
     1381 atcgcaatta aattaactta ttattgggta tttaataatt tcaaaaatac aaattggtga
     1441 caaaacgatc taggtgcgac cgaagctgtt gctaaaattg gcgtattgtt acacggagaa
     1501 ctcaacgata aaggccaagc agcacttgag ggaaaggtac gtaattgtag ctttaaatat
     1561 aatccaagtc ttaaaaatca tacaggggcg aatctttttg actatgccga tattagtgta
     1621 aagagttcaa ttattgacga tgatgttgac gaattcaacg ttgcttttaa tactattgct
     1681 gatgaaatta ctaccgaaac tccagaaggc tttgttcccg atggttcatt tttccaacat
     1741 ggaagacaaa ttcaaaatgc tagttatggt actgcgattt gccgtttagg aagaattatt
     1801 aaaatgattt ccgcatctaa taagtatttt tctgatgata aaatgactat tatcgaaaac
     1861 tatattttaa gtggtcttag atgttccatt cataaaggtt atataagtta tattcctgca
     1921 tcaagaacat atactcgtcc ggaaactccg cttgatgcaa aaatatttaa tacaaagcaa
     1981 cttgcttatt atttagacat tcctaatttt aagaataaaa ctacgctcag taattttatt
     2041 gaggacttaa agaataagaa ctctactttt gaaggaatca aatattttaa tgttgcaaaa
     2101 tttttaacta tgaatattga tgatgtttat atgtcgttta atggttccga taatacactt
     2161 attaattctg agtgcgtaaa taacgaaaat caacttggtc taaatttatc ttatgctaca
     2221 aacacatgtg ttatggatga tggtaaagaa tatgttaaca atggtccttt gtgaaagtat
     2281 gactatattc caggtacaac atcatttaat cttgattctg gcaaagaaga tgaacaactc
     2341 attgaaataa aaggcaatcc taatctttac gatgatccat tatttgaaac catgcttcct
     2401 gctcctgacg aaaatggaaa cattgtttat ggaggaggct ttagtgagga aaataatatt
     2461 gcttgtttaa tgcaaaagag tgtttctcat aaagaaaata aatttacggt tacctgtttt
     2521 ggctgtgaag atggaatggt tattgttggt tctgatcttg aatatactgg tgaggtaact
     2581 ggccctggaa agttttttac aactccacgc acaattcaca ctactttaga acagtgcaac
     2641 tataaaggaa gtcataaatt aagtgaggat ggaaaagaac ttactcatgg aaatgttgtt
     2701 tataagattt tagataacga taataaaatg caagttagca ctcaggaaat aactggttca
     2761 tgacaaagaa ataatcctga ctttaaaaat gaggtaaagc ctgtaactgg taacacattg
     2821 ttggcattta ttgagcatgg atcaaacaaa gctaaaggat atgcatattc aattcagcca
     2881 aaatcaagag aggataagaa atttgaatta attgatactg gcgatagcaa tattcatgct
     2941 gttaaattac ctaatggaaa agttgttgcg gctttctata agaatggaag ctttgattat
     3001 aatggtaata aatatgaagg ttcaattggg gaatataaga ttttctaatt aagtattaaa
     3061 gagcaaaaaa gagggcgcca atgacgccct cttagttttt tgtttagccc aattattttg
     3121 caagtcccag tttctttaaa gcatcttgct tttctttttc gatgcttgca agcaattctt
     3181 ctttttcatc ctgaatttct tcaagtctgc gtttattaat tgggaaccag aaaataaata
     3241 ataacattcc aacggcataa caaataagtg gaatatatgc gcctaagttc cagcacaata
     3301 taagttgatc tggacttggt gatcagtttg ttactacgta cctacccatt gcaattaatg
     3361 aaagattgac tgcaacaaat gataaacatt gagcaacttt tctaaagaac aacattaaag
     3421 aaagcgaagt tccgtcttcg cggactccat gttttacttc catatcatct acggcatcgg
     3481 caaccatgcc tcacattaac atggagaaaa agcttgtccc taaaccagat ataaagttta
     3541 acgcaacata gattcaatat accgattctg atgcgccagc gtaacgtaaa aataccataa
     3601 taaggtaaac ggttgtactt ataccaagac caaatacagt agtttctttt ttaccaaatt
     3661 ttttggacat tcatggtgta acaatcatca taataaatat ggctataata aatgaaaagt
     3721 ctggaagtgt ttgacgtcac cctgacattt caaagtaaat acgaactaca tatccagagt
     3781 aagcagtagt aaacattccg gcgccagcaa caatagctga aattacacaa attgaaagca
     3841 tagatctgga atgtgcaatt ctactaagag cccttttcat gcttccttta tcatgcttaa
     3901 cctgttttgc tgtatatcgt tcttttgtgc caaagtagca tagtattaat ataagaacag
     3961 taaatattgc taagatacca acaccaataa ttaagggctc tcattcaact ggtttatggt
     4021 ctgggtctgt tgggggatag aatgcatatg cttttacagc aagagcagga ataccaccaa
     4081 gttgtccaag aacacctctt catattgaga gcttaccacg ttcagatgca tctaaggtta
     4141 caacacttgc taaacttgag taaggaattt gaattgctgt catgcacata ccaaacaaga
     4201 tatatgtaaa tcctgcaata acatatgcag gtcacatact acccatgctt gctacaattt
     4261 tgtcaacgct atcagcattc aatgcaaaca ttaaaatact ggcaacggca aacggcatac
     4321 acattcataa tagatacctc ttaaatttac cagatctacc tggtctaatt ctgtcacaaa
     4381 tgcgacccat aataatgtcg ttaacagcat cccaaattct tgctacaaga aaaattacaa
     4441 gaataaagag tggattaaaa ccaagacagt cagaataata ggtctgtaat agtgaatttg
     4501 cgcaaccaaa aactaggcat gtaccaaagt ctccaattgc atagaagaag tagtccttta
     4561 ttttaattcc gctgtacttc gcaatagttt ttctttcttt ttgttccata aaacctccta
     4621 atttaaaaat gtcgaattct tgattttatt ttgaagttcg ttatagtaat cgtcttcgtt
     4681 aaccggaact tgaatagttt cgccattatg ccatcctgaa tatataatcg aattaattaa
     4741 ttcaacagca tcaagacctt cgttgccatg aattttcagc tcttcttttc caagcaatgc
     4801 gttggtgaag ttttgaatga ttcccttatg ctgaggattt tctccatcag tttcaacttc
     4861 aatttcttca acatttggac gatcataagg gccttcggca tttcgtgaaa attcctcagt
     4921 gtcagcatct aattttgcaa acaataattt atcgccttca actgtaagtt tgcctcgtgt
     4981 tccgctaatt tcaagtctat tgcatcctgg atattcacct gttgtggtta taaatattcc
     5041 agtagctcca tttttatatt ttaagaaagc agtaatttcg tcgtcggctt caatgtgatg
     5101 ccattgacca aaacctaaaa atccatttac gctttctggc atttctccaa caagtcattg
     5161 taataaatca atttggtggc aagcttgatt gatgatgagt ccgccgcctt cgccttttca
     5221 ggttgcacgt caaggagata cttcgtaata tttatctggc ctataccaac ttgtgataac
     5281 tcaatttatt ctttgaagct ttccaatttc ttcattctca atcatttctt tagctttacg
     5341 ataaacacaa tttgttcttt gattaaacat cattccaaaa agtgcctcgc tattctttga
     5401 agcttcaatc atctctttaa cttgctttgc ataaacccca gcaggttttt cacacataac
     5461 agatatgttt cgttttaaac attcaataac catttctgga tgaaaaggat gcggggaagt
     5521 aattattgcc gcatcaataa gttcgctatc aagcatttct ttgtagtctg tgaaatagct
     5581 aatgtcttgt ggtaaggctg gttttacagc ttcaattctt ttttcgttag gctcacaaat
     5641 cgcaacgata gttgcatttt caacttcttt gtttttaaga agcatttctg cataacagag
     5701 ttctgcgtga gctccaaaac caataattcc atacttaata gtactcattt aaactcctta
     5761 ttttatgaaa tttccctcat cggcttctgt atagccggct tgagaaagca ttattttagc
     5821 attttttact gaatccacaa aggcttcacc aacattttta tatacaaatt cacagctgtc
     5881 tttaatttgc tcaaaaggct ggtcagcagt ggcaaattta aggtggtgct caagactaaa
     5941 aaccatatct ttatcacaaa gatcaattag ctttgctatg ttagcttcgc cttttccgac
     6001 aggagtaatg tctgcatcat cattaacatg aataccatct ttgaaatgaa caaagtaagc
     6061 tcttggaaat acttctttta atgtcatctc agaaggctca ccaacttgta taaaattact
     6121 tgaatcataa accagtttta agtttggaat cttatcaaga agttctattg ttcgcttagc
     6181 ggtgtcagcg taagatcctt tttcattttc aagacagaca tttaaattaa attttgaagc
     6241 tttttcaaca cagagattca atcgctttaa tacttcgtca attttatcca gtgaatcaaa
     6301 gaaagaaaag acccggatat tctcacattc aaaaaactta gctttctcta tagcacgatc
     6361 tatatttgct tcaaattcag taattggaat aaataaatca cgtttaccaa ttggcgaaga
     6421 aatagaccat actttaatat tattttcagc aagcttttgt ttgcagtaat ttgcttcatc
     6481 gttgctaata tacaaaacac ttttatcaaa aataccacgt agctcggcta aatgaatacc
     6541 atttttattc attaaaaata cagcttcatc aaagtctttt cctgcttcgt cagttggagc
     6601 acataatcta atcataataa cccctaatat acatttttat tttttcgata atcttttcaa
     6661 accttttcaa agatttcttt atctcttgga attggcctga catttcttca ctttgcgata
     6721 aattttgaat tattaaattt gatttgtaaa atttttgttt tgaatttttt actgccactt
     6781 tctatcatgc taaatagaac agcagaagag cataacgcaa catcttgtaa tttataagct
     6841 tgtattaatt gagattttga ttttatagaa agctctgcat taaaattttt gattaagtta
     6901 agattttcct tcagaataat tttcatagta tcgaagttct ttactgcgcc ggcatacata
     6961 ctcatatttt tagcaatgct tttattttta ctattaacgt tacttgtttt actttttatt
     7021 tctttaaaga aattttggtg ttttaataag taattgtttg aaactctttt aaatttagaa
     7081 gcgctaattg gttttctttt gtcttccaaa atatattgag aagcacgaag cgacccaact
     7141 tgcccagcat ttaaggcaga tcctccggga cgtttaatgc catgtgtacc tgcgcattcg
     7201 ccaactgcaa atagtccttt aatatttgtt tgccatcatt tatctaccga aattccacca
     7261 tttaaatgtt gagagcataa tgcaatttgc aagtattccc ttgctaaatt aactcccttt
     7321 gacttgtaaa gctcaatagc gggtttgttt agctttgtaa gtctttttat tggcgtatct
     7381 tccaaagcat ttgtgttcgc taagtatgta tatgcttcct tgctcaattt tttgaagttt
     7441 attttcttta aaccgaaagg atttttctta aaatctaaat atacttttct tttgagatcg
     7501 tttgtttcat gatgtacaag taaatcaata accgaagatc cattttttgc tttttcttca
     7561 tcaaaaggtc attgatagcc tttcaaaaac aacattgata gagctttgta tttatctttg
     7621 tagtactcta gtaaaaattc gtgttctttt ccatccttat caacagaaat caatcttggt
     7681 aaaacttgca tataagaacc cgaaacattt cattttggtt ttaagcttgc aagtccatat
     7741 tgccattcac ttaaattttg taaatacgcg cctgccttta ttgcaataga agttgaacca
     7801 tgttgacttt ttggataaac attatcatca taaattgcag caggtccacc agtagctaaa
     7861 ataacatttg gagtattaaa tagcacatat tcattagttc ttacatccaa acataaaagc
     7921 ccagcaactt tatttgcatc tttaataatt tctacagcat ataatttatc atgaattttc
     7981 aggtcaagtt caaacgcctg cttctgaaga gcttccgtca taaattttga tgtcagagga
     8041 ccagcacttg ttgctcgctc aaagggatca tggtcggttt tataaccaat atattcacca
     8101 tatctatttg ttggaaatgg aacaccaagt tcgcataaat ttaaaaagca tcgggttgat
     8161 aaagctgctt cgcatagcgc gttatctcca tcaactgccc cgccatcaaa gatattttta
     8221 gccataagct taacgctgtc aagtgaattg ccagacaatg aaagcttata gtaagtttgt
     8281 ttgtcgcttc cagtatttct cgaagtgccg aagttcaggc cttcggtaat taaagcaaca
     8341 ctttttcttc catcttttag gatagcgtta gctgcattaa atccggcagc accagaacca
     8401 attacaacag catcgtatgt aaaagttttc gttgtcattt ataacctttg aatgttaaat
     8461 ccgccatcaa cattaattgc agttcctgta gaaaagtcaa gcaagccttg cgacacagct
     8521 aaaacagctt ttgcaacatc ttccgaagtt cctcaacgct tcattggtgt gatgccttcc
     8581 tcaataagtt tgtcatactt ttcagtaaca acttccgtca tatcagtttt gatgatgccc
     8641 ggacgaattt caattactgg aatattatat tcacctaatt tatcagcaaa aagctttgtt
     8701 accatagaaa gaccagcttt agatacacaa tattcaccac gagatacgct agaagtgtag
     8761 cttgaattcg atgaaatatt tacaattcgc ggttgatagt cttctaagtt atccttcaat
     8821 ttttcaatca tatagtttgc aaatatttgg cacataaaaa agttgccctt agtatttata
     8881 tctataagtc tgtcaaaact ttcttctgtt gtttctaaaa tgtcaagacg gtttttgcat
     8941 gcaactccag cattgttaac aagaaggtca acacgcccgt attttttgtc aacataatca
     9001 cgaagcttat ttctatcttc aggatttgag atgtcgcatt taatgtaatc aacgccctcc
     9061 attgagtcat gccttgcact ataaacaact gtatagccat tttcgctgag catatcagca
     9121 ataccttttc caatacctcg atatgaacct gttacaatag caactttttc taacattgca
     9181 ctttctcctt atataaattt ttatagtatt cgttaaatat agcacatcct gaaacttgtc
     9241 ccgctctcat tagttctgta cattttgaac aagccataca acaatttttc gaagaaaatt
     9301 ctccgttaaa ataatctgcg tagaaatttg gataggcaag agtcattcgt ccatacccaa
     9361 ttaaatctgc agcattattt tcaagcatat tttcagactt ttcaaacaaa tcatctctgt
     9421 aatatgaaag ccctgaattt atgaataaca ttttaggaaa tttttctttt aaagctttgc
     9481 caacttcaaa gaagcgtttg agcccatctt caggcgtctc ttgtggtttg taagcacctc
     9541 gtctaaaagg cctgttcaca tgaggattat aatacggatt tcctagcgtt atatttaaca
     9601 aagttaaacc aagcttttga agttcttcaa tgagcataaa tggttcagtt agatccatat
     9661 tgttattttc gtctgttcca aatccatatg gtttagggac catgtctgta acgctcaatc
     9721 ttgttgaaac aatcaaatta tttgaaacat tttccttaac ttttttaaag ctctttaagt
     9781 acaaacgagt tctgttttca aaacttccac cataatttcc aggacgcgta aaagatgaaa
     9841 gcaattcttg gaataaatag ccatggcatg atttaacgtc cacaccatca aagcctgctt
     9901 cttcggctaa ttttgcgcct tgcacatatt tatctggaag catgtctaag tattcgtcac
     9961 ttacgatatt ctcgtcgctt accggacgcg tttcttcgta aattggatga cgataagcaa
    10021 tcattggctt aatactttgt cttcctgaat gtgttagttg aataaagcac aatggctcaa
    10081 aaccaaattt gtctaaagtt gtctccttaa catctgatac taatttttta aaggtattaa
    10141 tgttattatt tgtcatcatc atttggcgag gattcgttct gccttcttcg caaacagcat
    10201 ttgcctcgaa tcaaataatc cccgcgccat tgttagcatt attaatatat tttttaattg
    10261 taagctccga aggcgagccg tccaagttgc aatcgcaacc ttccataggt tgagcaacaa
    10321 ctctattcgc acaaatcttg tcttcgatat taattttttc atttatcatt ggtcctactt
    10381 taatttaata gtgaatattt caaacggttt aacttcaaat tctaatttat tattttgaac
    10441 tctaagtttt ctttgttcat tttcaagtaa atcgcaaata tatgcttctt tgaagttaaa
    10501 tttcgtatta agatttaccc tttgtttttt gccttgacat tcaaaaccac gaagaatata
    10561 tgatttatct ttgttggcct ttttgaaagt ttctaaaacc acaccatttg aatttagtgt
    10621 aaggtaagta ttaacttcat taaaatattt ataattacca ggtttaacat gaagtattgg
    10681 tggtctgttt aatgcatatg cttgattaac tatatcgctg tttttgtaat tgcccttatg
    10741 cggataaatt gaaaaagtaa aatcaggaac atcgtctgtg gcatttggat tgggataatt
    10801 accagacttt acaattgtta gtgcaagatc atttccgttt acagaatggc catactttcc
    10861 attatttaaa agagcaacac catattttcc atcggacata tcaactcatt tttgagcgca
    10921 aacttcaaat tttgcttcgt caaatttatt ttgtggaatt gtattacggc caattgaacc
    10981 aaattgaaca tcacaacgca tttgtttggc cgctacatta actggaaaat ggacttttaa
    11041 catttgacgt ttttcatttc aatcaatgct atttttaaaa tctattctat taattttgtt
    11101 ataaagataa acgcgttgtt caaatttgct tttaccatag ctgcgaacaa tcttaaaacc
    11161 tttgcgatcg acttcgtcaa ttttcgtaaa ctttgcttgc gttttaatag gataattttt
    11221 ttgcttgtaa tatggactta tttcccagtt atctcactta aatggaatat cttcatagat
    11281 aacgaatttg ttaatcgccg aatttctcgc aacaacttgt tttttatttt tcttatcgta
    11341 tagataagtt atgtctccag aattattgaa cttaatttca aagaattcat tttgcattga
    11401 cttattgcca agtttaattt tattttcgca agttttcttt tcaatgcttt taaatgaaaa
    11461 tgccggtaga gttttaacga agcattttcc atcaatcata attggataat tgccttcaaa
    11521 ataatttgga ttaaagccaa tgtatttgct cttaaatttg gaagttaatt tcttcaatga
    11581 agagttaatt atttcattgt caattttctg gatttttgca aaatcaatac gagcatcgtc
    11641 ataaactttt ttgatagagc ttccaggtaa aatatcatga aattgattaa gtaataattt
    11701 tttataagct tcctcaatat ctttttgggg gtaagtagtt ttattttcaa ttgagtttag
    11761 tgatgccaaa atttcggtat tcgccatcaa aaattctgta tcacgatttg tttttttaat
    11821 tcatggaacc gaagtataag ttcctctgtg atattctaaa tataattcat cttttcaagt
    11881 tggtgtatgt ttatattttt tgcaacttgc attaaaatta ctttcgattt ctttaagagt
    11941 tgaagttagc gaagcagttc ttgtttttcc cattcctggc aagccataag aaaatcttcg
    12001 ttgtttttct aacatttctc gagtgggacc gccgcctcca tcgccgtagc catatgttgt
    12061 ataaacaaca ttattgtatt ttttattttg gtatctattt catgttccaa gaagttcgct
    12121 tgctagcata ttcccattat atgatgtacc gtctgtatca ttaactccat ttcgcggatc
    12181 agccgtgcaa gttgaaatta agtatgcaaa cacacttgtt ccgtccagcc caactcactt
    12241 aaatgggtta agtgggaatc tgttcgtatc atttcatcca atctttgttg tagaaaactg
    12301 tttgacgccg ctcttatcta aaatttgagg aatttgataa gcgtatccaa aaacatcagg
    12361 caaaaataat attttagaat caatgccaaa ctcctttttc atgaattctt tgccatataa
    12421 aatttggcgc accaaagatt cgccacttgt taaattgcaa tcaggttcaa ctcacattgc
    12481 gccttcaacc tcaaagcgcc cttccttaat tcgtttttta acagctgaat aaagtttagg
    12541 attatctgtt tttaaaaatt caaaaagctg aggcgaagta tggatgaatt tatattctgg
    12601 gtattcatcc ataaggttta gaactgtaga aaatgttcgt tgcgatttca aacgagtttg
    12661 ctcgatatct caaagccaag ccatatcaat atgactatga ccaacacaat tgatgaaata
    12721 gttctcttta ccaccttgtt caataaagta tttgttttta ataaactttt ttgcttttgc
    12781 taaagagtta aagaattttt cgtctttgtt acgcaaatca accatgttaa ttgcttcgtt
    12841 tacaatttta atgagctttg gatattgttt atttgtagga gtaattatgt ccaaattttc
    12901 aagcacacac aaaaaatcat aataagcttg ctcaattctt tcgtctttgt attttagtga
    12961 aaaatcaata ggataagctt tttcaaatac gtcagtatat agatacatat acatttcgta
    13021 ttttccattt ttgctgatct ttacttcaaa gtgattattg tcgattgctt gaattaattt
    13081 cccatttaga tataacatgc cctgaggagt taaaaaatcc tcgacattag taaagagctc
    13141 acggtttcca ttaatatata gtttgccatc acgtcttatt gctgtttttg caagcgaggt
    13201 aggcaagtcc acacaaaaat atgcgtttgt atatttatca attttattaa cgctgaaaga
    13261 acatttaaat caataatatg catcactttt atatataagt cgaggttgct taaacttttt
    13321 ataagcttta agttttttcg cattttcaag tattgggttt ttgcactctg cataagaaat
    13381 attcaagttt gaaattactt cagtagtttt aaactgattg agactgcgga caaatgcttc
    13441 cagtttctct tctattttat attgcattat gcctccttaa ccaaagttaa attatatcaa
    13501 aatctccctt tctttctgtc gtctctctat tgtgttgggt ataacaagct ttaatttctc
    13561 tatgtctaaa tttttaggtt tttggttgaa aatagcgtat tttttgctaa tatacgtggt
    13621 ccgacagaag cgaaggcaaa tagccttcca cctactggtt ga
//
LOCUS       DBNCDC010000019        13457 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_1619_length_13457_cov_7.205417, whole genome shotgun sequence.
ACCESSION   DBNCDC010000019 DBNCDC010000000
VERSION     DBNCDC010000019.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 13457)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13457)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13457
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1619_length_13457_cov_7.205417"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(77..829)
                     /locus_tag="AC04VQ_02520"
     CDS             complement(77..829)
                     /locus_tag="AC04VQ_02520"
                     /inference="COORDINATES: protein motif:HMM:NF019556.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HIO9553087.1"
                     /translation="MAGEYGGMNEVLEKVYEISKQTNALNADDLETLKRGSKGFDNQT
                     LFGNLTKNIDGIIGLHANQHIPQIIGSIFEHASSFEEGEQTTNHYFDIAKNFWEYVRG
                     RYAYSTGGVGKGEKFTNPYAQAASIGTEGEKDSNCETCCVYNLLKLTAQLNSYDPDNA
                     EYMDYYEKGLYNQILASQNPDVSSDAHNGVTYMLPIGPGGERGFGDDFYDFSCCCGTG
                     MENHVKYQEATYFKSKDNSTLYVGLYLPTTLN"
     gene            complement(914..1078)
                     /locus_tag="AC04VQ_02525"
     CDS             complement(914..1078)
                     /locus_tag="AC04VQ_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553088.1"
                     /translation="MLEMGAIYGGEGSGIWAPYYTFHKLMAGFIEAYEHAPNATVKNN
                     ALNLAKGSGL"
     gene            complement(1142..2227)
                     /locus_tag="AC04VQ_02530"
     CDS             complement(1142..2227)
                     /locus_tag="AC04VQ_02530"
                     /inference="COORDINATES: protein motif:HMM:NF019556.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HIO9553089.1"
                     /translation="MQKGSASASKAIDVSVKPKNPDEDIANEDLQYALNSATGIILEG
                     FVLPSEGPNGSTLSWSSDIDNIVVNDENSLDVTRPEENEDPVEGKLFVTATYKSAEVS
                     GNTYTKVVPIDTSKWINKISPIPDISVESGAVPRFPNFVDAELNDGTLVKAYVKWPTD
                     IDPSHIQQTYDGIIVGTNMTVTARVNVATNEARKEKQAELFELEDIDLTGDTFLKTNR
                     EHDLEYLKVLGQGVTDPSNGRFVMRILYNFYITFGIYNEDELQKLGIYELGGWEEPHG
                     LLRGHSVGHYISALGLAYASTQDAEIAVILERITTQLAEMQAMSSATSGKTSVDFKPS
                     NYDDNDPYGRAADFKERILSKALQVEN"
     gene            complement(2360..2914)
                     /locus_tag="AC04VQ_02535"
     CDS             complement(2360..2914)
                     /locus_tag="AC04VQ_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HIO9553090.1"
                     /translation="MDWYQRIFDFGTGTNNYLYVLNCGNNPGFENYATAINLSTGSKT
                     EEGVHSDSNLPDNAWCLVTVTMDKNGIIKLYKDKTLIDSKQTESRVKDLATAIISQQS
                     EDDSHCYVAASNYTSDACTAAKFCDFRLYNRALSEEEVATMYYPNDEALVQADANSLQ
                     LEQTQDLTSDFYLPAYGIYGSEIT"
     gene            complement(2930..3334)
                     /locus_tag="AC04VQ_02540"
     CDS             complement(2930..3334)
                     /locus_tag="AC04VQ_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553091.1"
                     /translation="MNKVISRSILIASVIFAVMAIFGIVCFAFANPGIIDHRYFNNVS
                     NDESLVCNYDFYMQNKESTTVPDVSGHGNAGSIKTRGDVRSYQILEDENVLGKKEWSF
                     QPNGGYGGTYLEVPTAAFETMTGDYAELTASI"
     gene            complement(3359..3733)
                     /locus_tag="AC04VQ_02545"
     CDS             complement(3359..3733)
                     /locus_tag="AC04VQ_02545"
                     /EC_number="2.7.1.26"
                     /inference="COORDINATES: protein motif:HMM:NF013821.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008531 - riboflavin kinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HIO9553092.1"
                     /translation="MDVICEIKSVVVMGNQLGRTVGMPTANFDSSKITAQLPKDGVYG
                     SIVTLPNGEEKFGVTNIGTKPSVDESGAHNVETHILDFDGDLYGQELSVRIVKHIRDI
                     KKFNSLEEVKAQVDLDKNWVII"
     gene            complement(3734..4576)
                     /locus_tag="AC04VQ_02550"
     CDS             complement(3734..4576)
                     /locus_tag="AC04VQ_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIO9553093.1"
                     /translation="MGRRDVVIKPTHSAGAVGVHICNTESQIRNGVENIVGQLDMFGA
                     VNDKALIQERIDGTEFIVNTVSCEGQNYVTAVYKYKKRMIPGGAMLYDINENIMDSEV
                     TPEIQGIMDYAQKVVKAIGIEYGPVHGEYMLDDEGPVLMEVNCRICGGDMSMKFLDVQ
                     FGHHETDRSLEAYLDEDKFTDRMKTGYKTAGKGYFKLLRVLESIDIVDAPICRIAEKM
                     PSFISYVGIEPNKKLHMTKTVDLYTNGGKFAFASHDAQQAKHDCMEIDRIEREDYDSM
                     WTLA"
     gene            complement(4583..5014)
                     /locus_tag="AC04VQ_02555"
     CDS             complement(4583..5014)
                     /locus_tag="AC04VQ_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553094.1"
                     /translation="MNKVIIVECISTSMNYVHDLRERGLEPVILETYNDDGGKEEARR
                     QENYVHMEKPLPRRIQALPNYEDTLELVRSENPILVLPGAEMSVELATNLAYDLGLKS
                     NNPENLPQMTRKSEMHEACKRAGLRYIRGQMVANAEEAIAF"
     gene            complement(5027..6490)
                     /locus_tag="AC04VQ_02560"
     CDS             complement(5027..6490)
                     /locus_tag="AC04VQ_02560"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012797837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIO9553095.1"
                     /translation="MIHINAWTTYGKKDVEKVMTFGEEYRQFISEYKTAREFSTGAIK
                     LAEEKGFKRLENLVEKGTKLKAGDKVYSQIQDRYTIMAVIGKKSMEKHGLNILGAHVD
                     SPRIDIKQNPLYQSDGVCMFETHYYGGISLYLWVDVPMSLHGIVCKKDGSKVTINIGE
                     KPDEPVFFINDLLIHLNHKDQEKPVKDFIDPEKMDLLVGSRPLVKDDVKSMKKYKGIF
                     EEKELVEKYSSASEPGNKSITQNILKILFDRYGIQELDLESAELEAVPIGPARDCGFD
                     SSMVMGYGHDDSACAYPSLRALLEMEKPEKTCMCVLVDKEEVGSLGATSMECRFFENT
                     IAEVMELCGESGMLKLRRAMSASSMISSDVSAAVDPNFHDKFEMMNSCFMGDGFVLNK
                     YGGSNGKKMSNDARPEFMAKLRKICNDNDVIYQTSELGKVGAGGGGTIAAIPANYGMD
                     VIDAGVAVLSMHGPWEVVSKADIYEAFKGYVSFLKDC"
     gene            complement(6555..7421)
                     /gene="ispH"
                     /locus_tag="AC04VQ_02565"
     CDS             complement(6555..7421)
                     /gene="ispH"
                     /locus_tag="AC04VQ_02565"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIO9553096.1"
                     /translation="MEICVAKNAGACYGVSRSLDVVERARNVSEKIATLGDLIHNPLV
                     IDDLKKSGVESVESVSEAKDKGIEKLIIRSHGISLEILNELKNTDIEIVDATCPYVKN
                     VSNAASLLASLYPAVIIVGKNGHPEVEGVASYICDAGAKCFIGENSEEINRFLPELKE
                     LNDSVGVVSQTTQSVEVFEDMIKYLKDNGVKVEVENTICVATKKRQESAAELAKQVDA
                     MLVIGGHNSSNTNHLADLCKENSEKVFHIESVEELKKVNLSGINKLGITAGASTPKSQ
                     IDKILDEIKTMS"
     gene            complement(7421..8209)
                     /locus_tag="AC04VQ_02570"
     CDS             complement(7421..8209)
                     /locus_tag="AC04VQ_02570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009608380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIO9553097.1"
                     /translation="MKTPKFWDKTSDEMPHFWGNLIFFVLRPIMIIFRLGHRNRENLR
                     TFQGKTGVVVISNHTSFLDVFCQYLCIRLKQWPRFLARETLYDHKALGFILSRCGAIP
                     IKRDSADRTALKRAAYLLKNKEIVGIMPEGTRRGKSGRKPTLHAGAAFIARMGGNVPI
                     LPATTINAEKVKQKGKFVRFPKITVAFGDPILLSDFDFLPKEDRLEACTWFAMRQSFS
                     MFYGCKPEEVDMVDLFPDTKDYSKIFEEHPIPHHTSEEIVKGLS"
     gene            complement(8196..8879)
                     /gene="cmk"
                     /locus_tag="AC04VQ_02575"
     CDS             complement(8196..8879)
                     /gene="cmk"
                     /locus_tag="AC04VQ_02575"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIO9553098.1"
                     /translation="MIIAIDGPSGAGKSTVSDEVAKILGFSHLDTGAMFRAVAYGALK
                     NAVDVTDEDGLAQIAENDKIVFETEDGNPAYKSILMAGEDITSQIRTDEVTKAVTPVC
                     KHPKVREALLRQQREIGKSGNYILDGRDIGTVVFPDAELKIFLDASAEERAKRRVLQN
                     EQSGVGSTDYDEVLADINRRDEEDSNRTVAPLRPADDAVQLDSTSKSKQEVIDEIVRL
                     AKEKQSENA"
     gene            complement(8879..10315)
                     /gene="aroA"
                     /locus_tag="AC04VQ_02580"
                     /pseudo
     CDS             complement(8879..10315)
                     /gene="aroA"
                     /locus_tag="AC04VQ_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012497.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
     gene            complement(10324..11151)
                     /locus_tag="AC04VQ_02585"
     CDS             complement(10324..11151)
                     /locus_tag="AC04VQ_02585"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIO9553099.1"
                     /translation="MSNQELRLQKYLARCGIASRRSCEKLITEGRISVNDKITTKMGT
                     KVDPENDVVKFDGEVIKPTNENITLMLNKPAGYVSTMNDPQGRPCVADLVPVDEYPSL
                     FPVGRLDRLTRGMLIFTTDGQLGQDLLHPKNEVGKKYEAVIDGDLTSSSEAYKKLTEG
                     VEILGGKTSPSKLNILEHFAFDKYLEFENEFFKNCAHKNDASALPKEKDLIDKKFSRV
                     EIEIHEGKNRQVRRMFEAVGFNVLLLKRISIGNLKLDNLECGKWAKLKDSDIELMKE"
     gene            complement(11144..11884)
                     /gene="scpB"
                     /locus_tag="AC04VQ_02590"
     CDS             complement(11144..11884)
                     /gene="scpB"
                     /locus_tag="AC04VQ_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009608394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIO9553100.1"
                     /translation="MIEDKDFESALEAVLFVCTDAVTTKHLAEIFEREASEVEEALQG
                     LQKRYEVEDKGIILKQIAGGWRLFSNPKFHEVIEKYVLSLDPRKLSAAALETLSIIAY
                     SQPVTRAGVASVRGVNSDSSINSLIEKGLVKEAGVADTAGSPILYTTTNKFLENFGLN
                     SIKDLPNIEDFAPDEETKRFIFERLSATQTDQLDQMEAFDSEVGDSSSEEKPELDSKD
                     MFESALAGAFGVTEKVNLDEIKLNTEDE"
     gene            complement(11900..12682)
                     /locus_tag="AC04VQ_02595"
     CDS             complement(11900..12682)
                     /locus_tag="AC04VQ_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIO9553101.1"
                     /translation="MAYKVKIENFEGPFDLLLQLVSDKKLDIGSISITSIIDQYLEQV
                     AHMQQVDLDVASDFMLVAATLLKIKTDMLLKLEPDELDEEFEEMTPSEARDTLVARLI
                     AYKQYKDVSDALQKLEDEQCMHFPRLAGWDPQYLNLMPNYLENVELVQLTQLAAGLFA
                     RRDEFLLDAAHIAAKPIPVVTYVKTIYTRIYNKKQMKFSDLIDASATPQIIVVNFLAM
                     LELYKHNYIDIKQTEHKSDIEIKYIEGSGALDLENIETDELD"
     gene            complement(12682..13368)
                     /locus_tag="AC04VQ_02600"
     CDS             complement(12682..13368)
                     /locus_tag="AC04VQ_02600"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIO9553102.1"
                     /translation="MNLPIFNTEYLIYVICSVLCFIPALVLHEVAHGYAAYKLGDPTA
                     KAAGRLSLNPLHHIDIFGTVVLPLFLMFFRMPVIGYAKPVPYNPSFFKDPRKGDLIVG
                     LAGPLANLVMALIAAGICWLITLFVPQAVQDNFVFQMFYVFFLNQFVMINLFLMFFNL
                     LPIPPLDGSSIIAILIPIRWLPKYYMIERYAMPIFLILIIVVPYILHVNPISLYLNAT
                     AGSIANLILP"
BASE COUNT         3905 a         3088 c         2385 g         4079 t
ORIGIN      
        1 cgcttcctcc cactttgatt gttgaagtct gcgaagggaa agtagtttct tgagttacgg
       61 ttactccctt ttctgctcaa ttaagagttg ttggcaaata aagtccaacg taaagagttg
      121 aattatcctt ggacttgaaa taagttgctt cttggtactt aacatgattt tccataccag
      181 taccacaaca acaactaaaa tcataaaaat catctccgaa gccgcgctcg ccaccaggac
      241 caattggcaa catatatgtt acaccattgt gcgcatcaga gcttacatca ggattttgcg
      301 atgccaaaat ttggttgtat agtccctttt cataataatc catatattca gcattatcag
      361 gatcataaga atttagttga gccgtaagtt ttaacaaatt gtaaacacag catgtttcac
      421 aattagaatc tttttctcct tcagttccaa tacttgcagc ttgcgcataa ggatttgtga
      481 acttttcacc tttgcctact cctcctgtcg aataagcata gcggccacgg acatattccc
      541 agaaattttt tgcaatatca aaataatgat tagtggtttg ttcgccttct tcaaatgacg
      601 aagcatgttc aaaaatagaa ccgataattt gtgggatgtg ctggtttgca tgaagcccga
      661 taattccgtc aatattttta gtcaaattac cgaaaagcgt ttgattatca aagcctttag
      721 atccgcgttt taaagtttct aaatcatcgg cattcagcgc atttgtttgt ttgctaattt
      781 cataaacctt ttcaagaact tcgttcattc cgccatattc tccagcaatg tatctatctc
      841 acattatttt tagttgagga agaccgccct cgtgctcatc atcatcaaga atcttacaaa
      901 ttcttgttgc tgctcaaagg ccagaaccct ttgccaaatt aagtgcattg ttctttacag
      961 ttgcgtttgg cgcgtgctcg taagcttcga taaagccggc cataagctta tggaacgtgt
     1021 agtaaggagc ccaaattcca ctgccttcgc caccgtaaat tgcacccatc tcaagcaaaa
     1081 caaattgatc gggcgaatat gaactaataa aacctttacc tcaatcctct ttattagtgc
     1141 ttcaattttc aacttgcaaa gccttggata aaattctttc tttaaagtca gcagcccgac
     1201 cataaggatc gttatcatca taattcgaag gcttaaaatc taccgaggtt tttccagaag
     1261 tcgccgaaga cattgcctgc atctcggcca attgtgttgt aatacgctca agtataactg
     1321 ctatttcagc atcttgtgtc gaagcataag caagtccgag cgctgaaata taatgcccaa
     1381 cagaatgacc gcgcaataaa ccatgaggct cttcccaacc accaagttca taaatgccta
     1441 atttttgaag ttcgtcttcg ttgtatatac caaaagttat atagaagtta tacaaaattc
     1501 gcataacaaa tctaccgttt gaggggtcag taactccttg gcctaaaacc tttagatact
     1561 cgaggtcatg ttccctatta gtttttaaga aagtatcccc ggtcaaatct atgtcttcaa
     1621 gctcaaatag ttcagcttgt ttctcttttc ttgcctcatt agtagcaacg tttactctgg
     1681 ctgtaacggt catgtttgtt ccaacaatta ttccgtcata tgtctgttgg atatgtgacg
     1741 gatcaatatc ggtcggccat ttaacatatg ccttgaccaa agtaccgtcg ttcagttcgg
     1801 catctacaaa atttggaaac cgaggcacag ctccagactc aacagaaata tcaggaattg
     1861 gagaaatctt attaatccac tttgaggtat caattggaac aacttttgta taagtgtttc
     1921 ccgaaacttc agcactttta taagtagcag taacaaacaa tttgccttct acaggatctt
     1981 cattttcttc tggtcgagta acatctaaag aattttcatc gttaactaca atattatcga
     2041 tgtcacttga ccatgaaagt gtcgaaccat ttggaccttc gcttggtaaa acaaaacctt
     2101 ccaaaatgat accagttgct gagttgaggg catactgcag atcttcgttt gcaatatctt
     2161 catcagggtt tttaggctta acactaacat caatagcttt cgaagcactt gccgaacctt
     2221 tctgcaagtt agcactaaga gtaacttgag ctgttggttg atcggcttcg gggcgaataa
     2281 cgtatgcaac ataatattct tcgccagttc atggatctgt aattttttca ccgatagtaa
     2341 cagcttcatt attgcttgct catgtaattt cagaaccata aatgccataa gccggcaaat
     2401 aaaaatccga agttaaatct tgcgtttgct caagctgtaa ggaatttgcg tcagcctgaa
     2461 caagggcttc gtcgttcgga taatacattg ttgcaacttc ttcctcactt aaagctctgt
     2521 tataaagacg aaaatcacaa aacttagcag cagtgcaggc atcacttgtg taattacttg
     2581 ccgcaacata acaatgcgaa tcatcttcac tttgctgcga tataattgct gtggcaagat
     2641 ccttaactct agattctgtt tgctttgaat ctattaaagt tttgtcttta tacaacttta
     2701 taattccatt tttatccatt gtgacagtca ctagacacca agcattatca ggcaaattac
     2761 tatcagagtg aacaccttcc tcagttttag agccagtact taaatttatc gctgtcgcat
     2821 agttttcgaa accagggtta ttaccgcagt ttaaaacata aagataatta ttagttcctg
     2881 taccaaaatc aaaaattcgt tgataccaat ctatatatgt aagattaact catatgcttg
     2941 cggtcaattc ggcatagtca ccagtcattg tctcaaaagc agctgttgga acctctaggt
     3001 atgttcctcc atagccacca tttggctgaa aggaccactc ttttttacca agaacgtttt
     3061 cgtcctccaa aatttgatat gagcgaacgt cgccacgagt tttaatggat ccggcatttc
     3121 catgaccaga aacatctgga acagttgtgc tctctttatt ttgcatataa aaatcataat
     3181 tgcaaaccag gctttcatca ttgcttacat tgttaaaata acgatgatca ataattcccg
     3241 gattagcgaa cgcaaaacat acaattccaa aaattgccat aacagcaaat atcacagaag
     3301 cgatcaagat tgatcttgag ataaccttat tcatatgaac tcctttgaag cttgacagct
     3361 atattataac ccaattttta tcgaggtcga cttgggcttt gacttcttcg agggagttga
     3421 atttttttat atctctaatg tgcttgacaa tgcgaacgga cagttcttgg ccgtataaat
     3481 ccccatcaaa atcgaggatg tgagtttcaa cgttgtgcgc gccagattca tcaacagaag
     3541 gctttgttcc aatgttggta acgccgaatt tttcttcgcc gtttggaagt gttacgattg
     3601 agccataaac accatctttt ggaagctgag cagtgatctt cgatgaatcg aaatttgcag
     3661 taggcatgcc gacagtgcga ccaagctggt ttcccataac aacaacagat tttatttcgc
     3721 aaattacatc catctaagca agtgtccaca ttgagtcata atcttcgcgt tcgatcctgt
     3781 caatttccat gcaatcatgc tttgcctgct gcgcatcatg gctggcaaaa gcaaactttc
     3841 caccattagt gtagaggtcg accgtctttg tcatatgaag ttttttattt ggttcaatac
     3901 caacataaga aataaacgaa ggcattttct ccgcaatcct gcaaattggc gcatcaacaa
     3961 tatcaattga ttccaaaaca cgaagcaact taaagtagcc tttcccagct gttttatagc
     4021 cagtcttcat gcgatcggta aacttatctt cgtcaaggta agcttccaag cttctatcag
     4081 tttcgtgatg cccaaactga acgtcaagaa acttcatgct catgtcgcca ccacaaattc
     4141 ggcaattaac ttccatgagg actgggcctt cgtcatctaa catatattca ccgtgaacag
     4201 ggccatattc aatgccaatg gcttttacaa ccttttgcgc gtagtccata attccttgaa
     4261 tttcgggagt gacttcgcta tccatgatgt tttcattgat gtcgtaaagc atcgcgccgc
     4321 ctggaatcat tctcttttta tacttgtaaa cagcagttac ataattttgc ccttcgcaac
     4381 ttactgtgtt tacaataaat tcagtgccat ctatgcgctc ctgaattaag gccttgtcat
     4441 ttacagcacc aaacatatca agctgaccaa caatattttc aacaccattg cgaatttgac
     4501 tttcggtgtt gcagatgtga acgcccacag caccagcact atgcgttggc ttaataacaa
     4561 cgtctcgcct gccgatgctc tctcaaaaag cgattgcttc ttcggcattt gcaaccattt
     4621 gccctcgaat ataacgaagt ccagcgcgct tgcaagcctc atgcatttca gatttgcgag
     4681 tcatttgcgg caagttttct ggattgttag atttaagccc taagtcataa gccagatttg
     4741 ttgcaagctc aaccgacatt tcggccccag gtaaaaccag aattggattt tcactgcgga
     4801 caagctcaag tgtgtcttcg taatttggaa gcgcttgaat gcggcgaggc aaaggctttt
     4861 ccatatgcac ataattttcc tgacggcgag cttcttcctt gccaccatcg tcattataag
     4921 tttccaaaat tacaggctca agcccacgct cacgcaggtc atgaacgtaa ttcatacttg
     4981 tggaaataca ctctacaatt ataactttat tcacaaaact cctaaattag caatctttca
     5041 aaaatgaaac ataacccttg aaggcttcgt aaatatcagc cttgctaaca acttcccaag
     5101 gaccatgcat gctaagaaca gcaacgccag catcgataac atccatgcca taattagcag
     5161 gaattgcagc gatagtgcca ccaccgccag ctccaacttt tccaagttca ctagtttgat
     5221 aaattacatc attgtcatta cagattttgc gaagcttagc cataaattcc ggacgagcat
     5281 cgttgctcat ctttttacca ttgcttccgc cgtatttatt caaaacaaat ccgtcaccca
     5341 taaaacatga attcatcatt tcaaatttgt cgtggaagtt agggtctaca gcagcagaaa
     5401 cgtcacttga aatcatcgac gaagcgctca tcgcacggcg aagctttagc attccgcttt
     5461 cgccgcaaag ttccataact tcggcgattg tattttcgaa gaaacggcat tccatacttg
     5521 tggcgccaag gcttcccacc tcttctttat caacaagcac acacatgcaa gttttttctg
     5581 gcttttccat ttctaacaaa gcccgaagcg aaggatacgc gcatgcacta tcatcatggc
     5641 cgtaacccat aaccatgctt gaatcgaagc cgcaatccct tgctgggcca attggaacag
     5701 cttcaagttc tgcagattcc aaatcaagct cttgaattcc gtacctgtca aacaagatct
     5761 tcaaaatgtt ttgagtgata gatttatttc cgggctccga agcgctcgaa tacttctcaa
     5821 ccagttcctt ctcttcgaag atacccttat attttttcat gcttttaaca tcgtctttaa
     5881 caagaggcct gcttcctact aaaagatcca tcttttcagg atcgatgaaa tctttaactg
     5941 gcttttcttg gtccttgtgg ttcaaatgaa ttagcaaatc attgatgaaa aatacgggtt
     6001 cgtcaggttt ttcaccaata ttaattgtaa ccttgctacc atctttcttg caaacaatac
     6061 cgtgcaaaga cattggaaca tcaacccaca aataaaggct tataccacca tagtaatgag
     6121 tttcgaacat gcaaactcca tcgctttgat agagaggatt ttgctttatg tcaattcttg
     6181 ggctgtcaac atgagctccc aaaatattaa gcccatgctt ttccatgctt ttcttgccga
     6241 taacagccat aatagtgtat ctgtcctgaa tttgagagta gaccttatct cctgccttaa
     6301 gcttggtgcc tttctcgacc aggttctcta gcctcttaaa acccttttct tcagccaact
     6361 tgatagcacc agtgctaaat tcgcgagcag tcttatattc gctgataaac tgcctatact
     6421 cctcgccgaa agtcatgact ttctcgacgt ccttcttgcc atacgttgtc catgcgttaa
     6481 tatgtatcat ttactacccc cacctttcat caaattatgt ggtaaatgcg gaattatact
     6541 acaaatcgat gaaattaaga catcgtctta atttcatcta agattttgtc gatttgggat
     6601 tttggggttg atgcacctgc tgtgattccg agtttattga tgcctgataa atttactttt
     6661 ttcagctctt ctacagattc aatatggaat actttttcgc tattttcctt acataagtca
     6721 gcgagatggt tggtgtttga ggaattgtgg ccgccgatga ccagcattgc atctacctgc
     6781 tttgccagtt cagctgcaga ttcctggcgc ttctttgttg ccacacaaat tgtattctca
     6841 acttcaactt ttacgccatt gtcttttaga tacttgatca tatcttcaaa gacttcaaca
     6901 ctttgagtgg tctgtgatac aacacctact gaatcattta attcttttaa ttcaggaaga
     6961 aatctattga tttcttcact gttttctcct ataaaacatt tagcgcctgc atcgcaaata
     7021 tacgaagcaa cgccttccac ctctggatga ccattcttac ccacaataat aactgccgga
     7081 tacaaacttg caagtaagct cgctgcgttt gaaacatttt ttacatatgg acaggtagca
     7141 tctactattt ctatatcagt attctttagt tcattaagga tttctaacga aatcccatga
     7201 cttcgaatta ttaatttttc tattccttta tctttagcct cagaaacaga ttctacactt
     7261 tctactccgc tcttctttaa gtcatcaatt actaaaggat tatgtataag atctcctaaa
     7321 gttgcaattt tttcacttac attacgagct ctctcgacta cgtcgagcga cctcgaaacc
     7381 ccataacacg cgccagcatt ttttgctacg caaatttcca ttaggaaaga cctttaacga
     7441 tctcttcaga tgtatgatgt gggataggat gctcttcgaa aatcttgcta tagtcttttg
     7501 tgtcagggaa taaatctacc atgtcgactt cttcaggttt gcaaccgtag aacatagaaa
     7561 aactttgacg cattgcaaac caagtgcagg cttccaggcg gtcttccttc gggaggaaat
     7621 cgaaatctga aagaagaatt ggatcgccaa aagcaactgt aatctttggg aagcgaacaa
     7681 acttgccctt ttgctttacc ttttcagcat taattgttgt tgccggaagg atgggaacat
     7741 tacctcccat gcgagctata aatgcagcgc cggcatgaag agttggcttc cttccgcttt
     7801 tgcctcggcg ggtgccttcg ggcataattc caacaatctc cttattcttc aaaagatatg
     7861 cagcgcgttt caatgcagtt ctgtcggcac tgtcacgctt aatagggata gcaccgcatc
     7921 tgcttaagat aaagcctaaa gctttatgat cataaagagt ttctctggcg agaaaccttg
     7981 gccattgctt caagcgaatg caaagatatt gacaaaaaac atctaaaaaa cttgtgtgat
     8041 ttgaaatcac aacaacgcct gttttgcctt gaaaggttct taaattttca cgatttctgt
     8101 gaccaagcct aaaaattatc attattggtc ggagcacaaa gaaaattaaa ttaccccaga
     8161 aatgaggcat ttcatcagaa gttttatccc aaaatttagg cgttttcact ctgcttttcc
     8221 ttagccaggc gaacaatttc atcaattact tcttgcttgc ttttcgatgt agagtctaac
     8281 tgaacagcat catcagcggg acgaagcggt gcaaccgtgc ggtttgagtc ttcttcgtca
     8341 cgacgattaa tatctgctaa aacctcatca taatcagttg agccaacacc gctttgttca
     8401 ttttgaagca cgcggcgctt cgctcgttct tcggcacttg cgtctaagaa aattttaagt
     8461 tctgcgtctg gaaaaacgac agtgccaata tcgcgaccat ccaaaatata atttccagat
     8521 tttccaatct cacgttgttg acgaagcaaa gcttctcgca cttttggatg tttacaaaca
     8581 ggagtgacgg ccttcgttac ttcgtcagtt cgaatttgcg aagtaatgtc ttcgcctgcc
     8641 atcaaaatac ttttataagc tgggttgcca tcttcggttt cgaaaacaat cttgtcattc
     8701 tcagcaattt gagcaagtcc gtcctcatca gtaacatcaa cggcattctt tagagcgcca
     8761 tatgcaacag cgcgaaacat agcgccagta tctaaatgcg aaaatccaag gatttttgca
     8821 acttcgtcgc taactgtaga tttacctgct ccacttgggc catcaatagc aattatcatt
     8881 atttgtcccc tttgctttcc aaaactaatt cattgaagtc atccaaaaat tgcggataac
     8941 taacatgcga aacttcgaag tgcctcatct tgaccggaac gtaagaacac aaacccataa
     9001 gcgcccaaca aattgcaaga cgatgatcat aatgatgctt gagcaaaacc acgccgtcgt
     9061 catctttatc aactttacct ttggcgcgct tggcttcgat gtcaattcca ccttcaacgg
     9121 ttaaagtatc agcggcgtcg cttgccttcg caccaagctt gcgaagcccc tcggaaattg
     9181 catgaattcg attgctttcc ttaagcttaa gttccttaac gccatagaag atgctttcgc
     9241 cttcggcaca tgcagctgcc atagccaaaa ttggaatctc atctatcatc gatggaattt
     9301 tatccgggct aacaacaatg ctatttaggc gaccggcata ttgaacagtg atgtccccca
     9361 caggctcgcc ttgataattg atatttttct ccaagcattc cttttcatcg agctcatgaa
     9421 tttcaatgcg cgagcccatc tctttcagcg catcaataaa cccaagacgc gttgggttaa
     9481 tcccaacatt atgaatggta atcttgcttc ctggaacaag tgccgcagca gttaaataat
     9541 aagctgccga agacacatcg cccggaattc aaatgtcaca agctttcaaa tccgctggac
     9601 cattaattgc aacacttaac atatctttat tgactttgat gtttgcacca aaagccttaa
     9661 gcaacatttc atcatgattt cttgactgga aaggttcaac gacggttgtc tcccccttga
     9721 tatttgcgcc ggcgaacaaa attgagctct taacctgcga agacgcaacc ggcatttcat
     9781 aatatatagg attgcaatcg ctgttgccaa taacttccat tggcaagctc gagcgctgag
     9841 gcttcccatt tttttgagca acaactctaa aatttgcacc catttgcata agcgggtcaa
     9901 caattctcat aaaaggtctg cggtgaagag attcgtcgcc atcaacaatc atcgcaatat
     9961 caaaaggagc aataactccc attccaatac gagcacaagt tgcagaattg ccacaataaa
    10021 ttgaagtctt tttagggtcc ggcccttttt tgccccatcc tgtaatttct aaatcaaagt
    10081 tgactgaatt tctgtgattc gaatcaagaa ttttcacctt cgcaccaagg tcgcgaacaa
    10141 cagcaacaac tgtcatgacg tcatttgacc tgggcaaatc aaatatatgc gaagtacctt
    10201 cagccattgc cgcaagataa atagccctac ttgcgatgga tttatcaccc ggaactgtca
    10261 aggaacctcg cagaggcata tttataggtt caaaagtttg atattcttcg ctcatattcc
    10321 ttcctattct ttcatcaatt caatatcgct atctttaagt ttagcccatt ttccgcattc
    10381 tagattatct aattttaaat tgccaatgct aatgcgtttc agcaacaaaa cattgaatcc
    10441 aacagcttca aacatacgtc gaacctggcg gttcttgcct tcgtgaattt caatctcaac
    10501 acggctaaat ttcttgtcga tcaaatcctt ctcttttgga agcgcagacg catcattctt
    10561 atgtgcgcaa ttcttgaaaa attcattttc aaattccaag tatttgtcaa aagcaaaatg
    10621 ctcaagaata tttaatttcg aaggcgaggt cttgcctcca agaatttcaa caccctctgt
    10681 caacttttta taagcctccg aagacgatgt caaatcccca tcaataactg cttcgtattt
    10741 tttaccaacc tcatttttag gatgaagcaa atcctgacca agctgaccat ctgttgtaaa
    10801 aatcaacata ccacgagtta acctatcaag ccttccaaca gggaaaagcg aaggatattc
    10861 atcaactgga acaaggtccg caacacaggg acgaccttga gggtcgttca ttgtgctaac
    10921 ataacccgca ggcttgttta acatcaaagt gatattttca ttagttggct taattacttc
    10981 gccgtcaaac ttaacaacat cattttcggg atcaaccttt gtccccatct tcgttgtgat
    11041 tttgtcattg acagaaatcc tgccttctgt gattaacttt tcacaggaac gacgcgaagc
    11101 aattccgcac ctcgcaaggt atttttgtag ccgaagctct tgattactca tcttcggtgt
    11161 ttaatttaat ttcatcaaga ttgacttttt cagtaactcc gaatgctcca gccaatgcac
    11221 tttcaaacat atctttagaa tcaagttctg gcttttcttc agaagagcta tccccaactt
    11281 cgctatcaaa tgcttccatt tgatcaagtt gatcagtttg agttgcgctc aaacgctcaa
    11341 agataaacct cttggtttct tcatccggag cgaagtcttc gatgttcggc aaatccttga
    11401 tagagttaag gccgaagttt tcaagaaact tatttgttgt ggtataaagg attggacttc
    11461 ctgcagtgtc agcaactcca gcttccttaa caagaccttt ttcgataagc gaatttatgg
    11521 agctgtcgct gttaactcca cggacagaag ccacgccagc gcgagttacc ggctgactgt
    11581 aagcgatgat tgacaaagtt tcaagtgctg cagcagataa tttacgagga tcaagagaca
    11641 aaacgtattt ttcaattacc tcatggaatt ttggattaga aaacaagcgc catccgccag
    11701 caatttgttt caagataata cccttgtcct caacttcgta gcgcttttgc aaaccctgaa
    11761 gagcttcttc aacttcgctt gcttcgcgct cgaaaatttc agccaaatgc tttgtggtta
    11821 cagcatcagt gcaaacaaac agcacagcct ctaacgcact ttcaaaatct ttatcctcaa
    11881 tcattatctc acctgcctgt taatccagct catcggtttc gatgttctct aaatcaagcg
    11941 cacctgagcc ttcaatatat ttaatctcaa tatcactttt gtgctcagtt tgcttaatat
    12001 caatatagtt gtgcttataa agttcaagca ttgccaaaaa gttaacaaca ataatttgcg
    12061 gagtggccga agcatcaatc aagtcagaaa acttcatctg ctttttgtta tatattcgag
    12121 tgtaaattgt cttgacataa gtaacaaccg ggattggctt tgctgcaata tgcgcagcgt
    12181 ccagtaaaaa ttcatcgcgt cgcgcaaaca gaccagctgc caattgtgtg agttgaacaa
    12241 gctcaacatt ctccaaataa tttggcatca aatttaaata ttgagggtcc caaccagcca
    12301 aacgaggaaa atgcatgcac tgctcatcct ctaatttttg aagcgcatcg gacacatctt
    12361 tgtattgttt ataagcgata agccttgcaa ccaatgtgtc gcgagcttcg gatggtgtca
    12421 tttcttcgaa ttcttcgtca agttcatctg gctcaagttt gagcaacata tcagttttaa
    12481 ttttgagaag cgttgccgca acaagcatga agtcacttgc aacatccaaa tcaacttgct
    12541 gcatatgagc aacttgctct aagtattgat cgataatgct tgtgatagaa attgaaccaa
    12601 tgtcaagctt tttatcgctg accagctgca acaaaagatc gaacggacct tcgaagtttt
    12661 ctatttttac cttatacgcc attatggaag aattaagtta gcgatgctcc ccgccgtcgc
    12721 atttaggtat aacgaaattg gatttacgtg caaaatataa gggacaacaa ttatcaaaat
    12781 caaaaagatt ggcattgcat aacgctcaat catgtaatac ttcggaagcc aacgaattgg
    12841 aattaaaatt gcgatgatgc ttgagccgtc caaaggcgga attggaagca aattgaagaa
    12901 catcaagaac aaattaatca tcacaaattg attcaagaaa aatacataaa acatttgaaa
    12961 tacgaagttg tcttgcaccg cttgcgggac aaacaaagta ataagccagc aaattcctgc
    13021 agctatcaat gccataacga ggttcgccaa aggacctgcc aagccaacaa ttaaatctcc
    13081 tttgcgagga tctttaaaaa acgaaggatt atatggaacc ggcttcgcgt aaccaataac
    13141 tggcatccta aaaaacatca agaaaagtgg caaaactact gtgccaaaaa tatcaatatg
    13201 atgcagtgga tttagtgata gtcttcctgc tgcttttgca gttgggtccc ccagcttata
    13261 agcggcataa ccatgcgcaa cttcgtgcaa aactaaagct ggaataaagc ataaaacact
    13321 acaaataacg tagattaaat attctgtgtt aaaaattggt aaattcattt acaggatatc
    13381 ctcctcgaca tgctgtcttt cctggcggag agccggggat tcgaaccccg gatacccttg
    13441 tggggtacac tcgctta
//
LOCUS       DBNCDC010000020        12585 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_1848_length_12585_cov_6.900399, whole genome shotgun sequence.
ACCESSION   DBNCDC010000020 DBNCDC010000000
VERSION     DBNCDC010000020.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 12585)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12585)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12585
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1848_length_12585_cov_6.900399"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /locus_tag="AC04VQ_02605"
     CDS             <1..144
                     /locus_tag="AC04VQ_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HIO9553103.1"
                     /translation="GVKVSGATVHFVNEIPDGGRIIAQKAVNVEDGDTPETLQKRIME
                     QAE"
     gene            318..1403
                     /locus_tag="AC04VQ_02610"
                     /pseudo
     CDS             318..1403
                     /locus_tag="AC04VQ_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013431.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
     gene            1419..2141
                     /locus_tag="AC04VQ_02615"
                     /pseudo
     CDS             1419..2141
                     /locus_tag="AC04VQ_02615"
                     /inference="COORDINATES: protein motif:HMM:NF019443.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
     gene            2151..3335
                     /locus_tag="AC04VQ_02620"
     CDS             2151..3335
                     /locus_tag="AC04VQ_02620"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012799495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HIO9553104.1"
                     /translation="MKELELKYGCNPNQKPSKIFMHDGSELPIEIMNGKPGYINFLDA
                     FNSWQLVKELKNILGLPAATSFKHVSPTSAAVAVPLSEDLKKAYFVDDIEGLDDSPIG
                     MAYARARGTDRLCSFGDFIALSDTCDEVTARMIAREVSDGIIAPDYTPEALEILRSKR
                     KGNYNVVKIDPNYVPAEKEYKQVFGITFEQGRNNFVIDEALLGDIVTENKDLPDAAKR
                     DMILSLITLKYTQSNSVCFAVDGQAIGVGAGQQSRIHCTRLAGSKADTWWLRQHEKVL
                     ALPFKEDIGRPERDNAVDAYINKNEEDILADGVWERYFTEKPAEFTDEEKRQYLDSVD
                     GVSLGSDAFFPFGDSIERGKKSGVKYVAEPGGSIRDDQVIETCNKYNMVMAFTKMRLF
                     HH"
     gene            3346..4605
                     /gene="purD"
                     /locus_tag="AC04VQ_02625"
     CDS             3346..4605
                     /gene="purD"
                     /locus_tag="AC04VQ_02625"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIO9553105.1"
                     /translation="MKIMVIGSGGREHAIIKSLRKSAGVDEIYCLPGNGGIAQDATCV
                     DIGATDIDNIVKFAKEEAIDYAVVAPDDPLVLGCVDALNSEGIQCFGPTANAAIIEGS
                     KVFSKNLMKKYGIPTASYEVFDDPAKALEYVEEAPLPTVIKADGLALGKGVIIAEDRD
                     AARQAIREIMENKKFGDSGNQIVIEEFLEGPEVSVLSFTDGKTVKPMVSSMDHKRAKD
                     NDEGLNTGGMGTVAPNPYYTDEVAERCMKEIFLPTIDAMNAEGRTFKGCLYFGLMITK
                     DGPKVIEYNCRFGDPETQVVLPLLEGDLLEIMQACTEGTLDSVSVDFVDKHACCVIAA
                     SDGYPESYEKGFELTIPEECKDSVFVAGAVAEGDKLLTNGGRVLGCTAIADSLQEAID
                     KAYEMVEKVEFENKFYRTDIGKRALKA"
     gene            4605..5525
                     /locus_tag="AC04VQ_02630"
     CDS             4605..5525
                     /locus_tag="AC04VQ_02630"
                     /inference="COORDINATES: protein motif:HMM:NF037180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553106.1"
                     /translation="MVYRVYVEKKPEFAHEAKALLKDAKELLGVKGLEDVRIFNRYDA
                     ENLSEPDFEKAILNVFSEPQVDNVYRTVDEGDAEVFGIEFLPGQFDQRADSAAQCIQI
                     MSQGQRPIVKSAKVIALYGKLTEDDLAKIKKHMINPVEAREASLELPETLQIEYEIPT
                     EVEILNGFNEKSEEELQAFIKDNGLAMDFGDIKFVQDYFKSIDRDPTITEIKVIDTYW
                     SDHCRHTTFNTIIDNIKFEDELLEKTYNEYLKTREELGRTKPITLMDIGTIAAKYLRK
                     NGKLDKLDESEEINACTVKMDVEVDGKVEP"
     gene            5529..6671
                     /locus_tag="AC04VQ_02635"
     CDS             5529..6671
                     /locus_tag="AC04VQ_02635"
                     /inference="COORDINATES: protein motif:HMM:NF014788.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HIO9553107.1"
                     /translation="MLFKNETHNHPTEIEPFGGAATCIGGAIRDPLSGRSYVYGAMRL
                     TGASDPTVPISETLPGKLPQRTIVTTAADGYSSYGNQIGLATGIVDELYHPGYVAKRM
                     EIGAVVAAAPAKNVRRERPAAGDAVILLGGATGRDGIGGATGSSKAHDSHSVETCGAE
                     VQKGNAPEERKLQRLFRNEEASKMIKRCNDFGAGGVSVAIGELADGLNIDLNKVPKKY
                     EGLDGTELAISESQERMAVVVAAEDEKRFLELAKSENLNAIAVATVTEEPQLVLNWNG
                     KKIVDISREFLDTNGAPKHIDIETEKTVKPNKKVEGSFVELYKNLADDLNVCSKRGLA
                     ERFDSTIGAGTVLMPFGGKKQLTPIQAMVQKVSVEKKHTDNCSVMS"
     gene            6852..8294
                     /locus_tag="AC04VQ_02640"
     CDS             6852..8294
                     /locus_tag="AC04VQ_02640"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HIO9553108.1"
                     /translation="MLGAYKAQVELEVAAIGGKDSMSGSFEDIHVPPTLVSFAVTTDK
                     VKNIVSPEFKTAGNKIVILKPEYDENGLPKIDSLKKNFETVTKLMREGKVVSCWTPGF
                     GGIAEAILKMSLGNDFGFSFDENISTDELFNYSYGSFVMETTADIKDAKLVGTITDDK
                     TFNLGKDAIKTSELLEIYENKLEDIYSCNIKHSKNNIEDFTYEAKEWPKPAIKVAKPR
                     VLIPAFPGTNCEYDTAKAMEDAGCEAKIFIVNNLNQDSLKKSIEDFAAETNKSQIIFI
                     PGGFSGGDEPDGSAKFITSFFRNAEVKDAVTDLLDAREGLMAGVCNGFQALIKLGLVP
                     YGKIVEPNEDAPTLTYNEIHRHQSRVVHTRIASNKSPWLANLNAGDIINVVVSNGEGR
                     FIANEKDIQELAKNGQIATQYVDLNGKATDDIHFNLNNSMCAIEGITSPDGRVFGKMG
                     HAERYAPGLYKNVPGNFDYKMFEAAVKYFK"
     gene            complement(8428..8712)
                     /locus_tag="AC04VQ_02645"
     CDS             complement(8428..8712)
                     /locus_tag="AC04VQ_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553109.1"
                     /translation="MLEVIDEDTPVTVFVGEDAQRPLSERVVKFLGKVNKNHFVIDTK
                     DYDMPGIDEKYRGSISHHILRAVNNRVDAYMELFLEHPLNLRRYYRQIEY"
     gene            complement(8770..9279)
                     /locus_tag="AC04VQ_02650"
     CDS             complement(8770..9279)
                     /locus_tag="AC04VQ_02650"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase family protein"
                     /protein_id="HIO9553110.1"
                     /translation="MRGKSNVPVYVENAAEYLTTGNKKLTDKSLVVYSTVSGTTAEMI
                     EFCKAVKAQGTRIFAFVDTPDTELTKPEYQDYLICYPENEQLKFYMVGNYLMFKNGDF
                     PDYDEYNKQMEASLAEGLANIEEAADELGFNFAKSEVERIKKNPAYPRYFVGAGNHYG
                     ATYSYAMCY"
     gene            complement(9307..9528)
                     /locus_tag="AC04VQ_02655"
     CDS             complement(9307..9528)
                     /locus_tag="AC04VQ_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553111.1"
                     /translation="MDKNHPSSVESKQKIEALKAESKESPSLRNFDRQKKIDSINGAL
                     ALRPKIEEILDKVWDDGFDNIFFVGIGGT"
     gene            complement(9538..10332)
                     /gene="frlD"
                     /locus_tag="AC04VQ_02660"
     CDS             complement(9538..10332)
                     /gene="frlD"
                     /locus_tag="AC04VQ_02660"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: protein motif:HMM:NF007321.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HIO9553112.1"
                     /translation="MKLAAIGSNCIDFYADIEGGRAYPGGGPVNMAVYVKRLGHESSY
                     IGHIGNDKFGDLMKASIAERGVDISHLHVMEGKTAVTQVKLDNGERILGDYDEGVLAN
                     YRLDNDDIDFICKHDIVVCDLWGKVEDQIETLHNRGIKTAFDCATDPGDDACKVAIPH
                     TDYLFFSNDNTSTEELRLIMKELRSKGPQLIIAMRGEKGSICFDGKEFYEFGIVKCDN
                     VVDTMGAGDSYIAGFLSAIGEAAQIDEAMKIGALTATETIQYFGAW"
     gene            complement(10333..11748)
                     /locus_tag="AC04VQ_02665"
                     /pseudo
     CDS             complement(10333..11748)
                     /locus_tag="AC04VQ_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(11813..>12585)
                     /locus_tag="AC04VQ_02670"
     CDS             complement(11813..>12585)
                     /locus_tag="AC04VQ_02670"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006362087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIO9553113.1"
                     /translation="DRIPALVEAGADVLCIDSSEGYSDWQKKTIEWTRKKYKNKVKIG
                     AGNVVSAEGFRFLVNCGADFIKVGIGGGSICITRETKGIGRGQATALIEVAKERDKYF
                     KKTGIYVPICSDGGIVYDHHITLALAMGADFVMLGRYFARFDESPSERVNIGGTYYKE
                     YWGEGSTRARNWQRYDMGGKKGLSFEEGVDSYVPYAGALQDNVRASLAKVKSTMCNCG
                     SLTIPQLQKNAVITLVSSTSIREGGAHDVIQKNKNISK"
BASE COUNT         3963 a         2660 c         2613 g         3349 t
ORIGIN      
        1 ggcgtcaaag ttagtggcgc gacggttcac ttcgtcaatg aaattccgga cggtggacgt
       61 atcatcgctc aaaaagctgt aaacgtcgaa gacggagaca cccctgaaac acttcaaaaa
      121 cgcataatgg aacaggccga atgaataata cttccgcaag caactgaaaa agtttgtagc
      181 gagattatta gggggcaatc ataaatatta caagaagaaa ttttattttt ggaggagcag
      241 ctacgcttgg cgcaatcgcc gcttcgccgg ctctatcatc ttgttcaagt aatacaatca
      301 ctaagtcggg actgaaaatc gcaaacttcg aaagcgatgg cacggcagca tttaataccg
      361 gacttcaaaa cacatacaca tcgaatgtcc caacaacttt atgtgttctt acaaacaaaa
      421 acggcatgga ggcatgcatc acaaatcttg gtgcaagact tgtgtcgcta atggttccgg
      481 ataaaaatgg agacttcaaa gatgtttgct taggcttcga caacattgca gactacgcaa
      541 acatcgacaa gcaaaataat gtaatgggtg ccgaagttgg tcgaaccatt aaccgcatcg
      601 ctaatgcaaa atttacatat gacggaattg aatatcaact tgagaaaaat tatcgcgaca
      661 agcataacat tcatggtgga aaatttggtt ttcactatca aacatgaagc atcgaaaaac
      721 taacttcaga ttcggtcacg ctaagacttg tttcgccaga cggagacgga ggcttcccag
      781 gagaaatgaa tgttgctgta acctatacgc tagggccaga aaatgattta tgaatcgacg
      841 tgatggctca aactacaaaa gcaagtcctt tcaacgttgc aaatcatacc tactttaatc
      901 ttgaaggcga cccaacaaaa gattgctatg actatcaaat tcaaatattt tcaaataaat
      961 atattcctgc cggagaagat gtaataccac aaaacgaaat cctcattata cctgagggcg
     1021 acgtgcttga tttttactca aagcctaaaa gcatgtccaa aaacatcgat acttcgcaag
     1081 gaatttttca acacacgaag ggttatgact tcccttttat tcttgaagct aacagaacaa
     1141 ttaatgctac tgcatatagt cctaaatctg gaattaaaat ggaagttata acagacaggc
     1201 ctataataaa cttttatgat gcgcacgatc ttgatggcac tatgattggc aaaaacaaca
     1261 ttccttataa cgctatgtgt gcgatttgct tggaaaccca agacatgtct gatggcgcta
     1321 ataaaaacga ctttgcgaat acaattttaa aaccggggca atggtatacc tcctcggtcg
     1381 aatataaatt ttcagttgaa taattttagg aggcatttat ggaagacatt tacaaaatta
     1441 acaacatcga agatttacta agcaacaata cttatccggg gcgcggactt attgttggca
     1501 aaactccgga cggcacaaaa tcggtcgccg catattttat tatgggcaga agcgaaaaca
     1561 gccgcaaccg tatcttcgtt gacaaaaacg aaacaatttt cactgagcca ttcgacccaa
     1621 gcaaggttga agacccaagc ctaattattt atgccgcggt tagacatttt gaaaagaatc
     1681 taatagttac aaatggtgat cagaccgaca caatttacga tggacttcaa gccggcaaga
     1741 cttttgctga aagtcttgaa agccgcgact tcgaaccaga tggtccaaat tgaactccaa
     1801 gaatttcggc gcttctaact tatgctaacg aaaattttga ttaccaaatg aatattttaa
     1861 agagcattga cgccgaaggc agcgcgaccg ctcgccacac ttatcacttc ccaagcgttg
     1921 ctggacttgg tcatttctta cacacttatg tatgtgacgg aaatccaatt ccaactttcc
     1981 agggtgagcc agagcgtatt gcaattgata acgacattga aactttcact gacaaagttt
     2041 ggaattcgct aaatgctgac aataaaattt ctctttgggt aatgtttgcc gatctcaaaa
     2101 ctggcgaaac tcagaaaaaa attattaaca aatatatgta aggagacgca atgaaagaac
     2161 tcgaactcaa atacggatgt aatccaaatc aaaagccttc aaaaattttc atgcacgacg
     2221 gctctgaatt gccaattgaa attatgaatg gcaaaccggg ttatatcaat ttcttggatg
     2281 cttttaacag ctggcaactt gtcaaggaac ttaaaaatat tttaggactg cctgctgcga
     2341 cttcgtttaa gcacgtaagt ccaacttcgg cagctgttgc tgttccttta agcgaagatt
     2401 taaagaaggc ttactttgta gatgacatcg aaggtcttga cgactctcct attggcatgg
     2461 cctatgctcg tgcgcgcgga actgacagac tttgttcttt cggagacttc attgcgctta
     2521 gcgatacatg tgacgaagtt acagcccgca tgattgctcg cgaagttagc gacggaatta
     2581 ttgctccaga ttacactcca gaagctcttg agattcttcg ctcgaagcgc aaaggcaact
     2641 acaacgttgt gaaaatagac cctaactatg ttccagctga aaaagagtat aagcaagttt
     2701 ttggaattac ctttgaacaa ggtcgcaata acttcgtaat tgacgaagca cttcttggtg
     2761 acatcgttac agaaaacaaa gacctccccg acgccgcaaa gcgcgacatg attttgtctt
     2821 taattacact taaatatacg caatcgaatt cggtttgctt tgcggttgat ggtcaagcaa
     2881 ttggcgttgg cgctggtcaa caaagccgta ttcattgtac tcgtcttgct ggcagcaagg
     2941 ctgacacttg gtggttacgc cagcacgaaa aagtgctggc gcttcctttc aaagaagaca
     3001 tcggccgtcc tgagcgtgat aatgcagtgg atgcctacat taataaaaac gaagaagaca
     3061 tcttggctga cggagtttgg gagcgttact tcacagaaaa acctgcagag tttactgacg
     3121 aagaaaagcg ccaatactta gacagcgttg atggtgtaag tcttggaagc gatgcgttct
     3181 tccccttcgg agacagcatt gagcgcggaa agaaatccgg cgttaaatat gttgccgaac
     3241 cgggtggatc aattcgcgat gaccaagtta tcgagacttg caataagtac aacatggtaa
     3301 tggcatttac gaagatgagg ttgtttcatc actaaaggag actttatgaa aattatggta
     3361 attggttcag gaggtcgcga acacgccatc atcaaatcgc tccgaaagag cgcgggcgtt
     3421 gacgagattt attgtctgcc gggtaatggt ggaattgctc aagatgcaac ttgtgttgac
     3481 atcggcgcaa ccgacattga taacattgtt aagtttgcca aagaagaagc gatagattat
     3541 gcagttgtcg ctccagatga tcctttagtt ttaggctgcg ttgatgctct gaattccgaa
     3601 ggaattcaat gtttcggccc aactgcaaat gccgcaatca ttgaaggctc taaggttttt
     3661 tccaaaaacc taatgaaaaa atatggcatc ccaactgcat catatgaagt ttttgatgat
     3721 cctgcaaaag cacttgaata tgtcgaggaa gctccgcttc caactgttat taaggctgac
     3781 ggactcgcat taggtaaagg tgtaatcatt gctgaggatc gcgatgctgc gcgtcaagca
     3841 attcgagaaa taatggagaa caaaaagttt ggtgacagtg gaaaccaaat tgttattgag
     3901 gaattcctcg aaggcccaga agtttctgtc ctgtcattta cagacggtaa aactgtcaag
     3961 ccaatggttt cgtctatgga tcacaaacgt gctaaagata atgacgaagg tttaaacacc
     4021 ggaggcatgg gcaccgttgc tcctaatccc tattacaccg atgaggttgc agagcgctgc
     4081 atgaaagaaa tattcttgcc aacaattgat gcaatgaatg ccgaaggcag aaccttcaaa
     4141 ggctgcttat attttggtct aatgattaca aaagatggtc ctaaagttat tgaatataac
     4201 tgtcgcttcg gcgatccaga aactcaagta gtcttgcctc tcctggaagg tgatttatta
     4261 gagataatgc aagcttgtac cgaaggtact ttagacagcg taagtgtcga ctttgtcgac
     4321 aagcatgctt gctgcgttat cgcagcaagt gatggttacc ctgaaagtta cgaaaagggt
     4381 tttgaattaa caatccctga ggaatgcaaa gactccgtct tcgtggccgg cgcagtcgcc
     4441 gaaggtgaca agttattaac taatggtggt cgagtacttg gatgtacggc tattgcagat
     4501 tcacttcaag aagcaattga taaagcttat gagatggttg agaaggtaga atttgagaac
     4561 aagttctacc gaacagatat tggaaagaga gcgttaaaag cataatggtt tatagagttt
     4621 atgttgaaaa aaaaccagaa ttcgcacatg aggcaaaagc cttattaaaa gatgcaaaag
     4681 aattgttagg cgtcaaaggt ttggaagacg ttcgtatctt caatagatat gatgccgaaa
     4741 atctaagcga gcctgacttc gaaaaagcaa tcttgaatgt ctttagcgaa ccacaagttg
     4801 acaacgttta tagaacggtt gacgaaggcg acgccgaagt cttcggcatt gagtttttgc
     4861 ccggacagtt cgaccaacga gctgatagtg ctgctcaatg cattcaaatt atgagccagg
     4921 gacagcgccc tattgtcaaa agcgctaagg ttattgcact ttatggaaaa cttacagaag
     4981 acgacctcgc taaaattaaa aagcatatga tcaatccggt ggaggcccgt gaagcttcgt
     5041 tggaattgcc tgaaactttg caaattgaat acgaaattcc aactgaagtc gaaatcttga
     5101 atggttttaa cgaaaaatct gaagaagaac tccaagcctt cattaaagat aatgggctcg
     5161 caatggactt cggagacatt aaatttgttc aggattattt caagagcatt gatcgcgacc
     5221 caacaattac tgagattaaa gtaattgaca cttattggtc agatcactgt cgccacacaa
     5281 cttttaacac gattatcgac aacataaagt tcgaagatga gcttttagaa aaaacttata
     5341 acgaatattt aaaaactcgc gaggagcttg gccgcacaaa accaatcacc cttatggata
     5401 ttggcacgat tgcagcaaag tatcttcgca agaatggtaa attagacaag cttgatgaaa
     5461 gcgaagagat taacgcgtgc acagttaaga tggacgttga agttgatggc aaagtcgaac
     5521 cttgactttt gcttttcaaa aacgaaaccc acaaccaccc tactgaaatt gaaccatttg
     5581 gtggtgctgc aacttgcatt ggcggggcta ttcgcgaccc cctttcagga agaagttatg
     5641 tctatggcgc tatgagatta acaggcgctt ctgatccaac tgttccaata agcgaaactc
     5701 tgccaggcaa gttgccacaa agaacgatag ttacaacagc agccgatgga tattcttctt
     5761 acggcaacca gattggttta gccactggaa tcgtagatga attgtaccac ccaggctatg
     5821 tcgccaaaag gatggaaatc ggcgctgttg ttgctgcagc tccggcaaaa aatgttcgtc
     5881 gcgaacgtcc agccgctgga gatgctgtaa tacttcttgg aggagccact ggccgcgatg
     5941 gaattggagg cgcgactggg tcttcgaaag ctcacgactc tcacagcgtt gagacttgtg
     6001 gtgccgaagt tcaaaaaggt aacgctccgg aagaacgcaa acttcaacgt ttgtttagaa
     6061 acgaagaagc cagcaagatg attaagcgat gcaatgactt tggagctggc ggcgttagcg
     6121 ttgcgattgg cgagcttgcc gatggcctta acatcgactt aaataaggtt cctaaaaaat
     6181 acgaaggcct tgatggtacc gagcttgcaa ttagcgaatc tcaagagcgc atggccgttg
     6241 ttgtggctgc cgaagacgaa aaacgcttct tggagcttgc caaaagtgaa aacttaaacg
     6301 ctattgcagt tgctacagta actgaagaac ctcagcttgt tctcaattgg aacggcaaga
     6361 aaattgtgga tataagcaga gagtttttgg acaccaatgg tgctccaaaa cacatcgata
     6421 ttgaaactga gaaaaccgtt aagccaaaca aaaaagttga aggtagcttc gttgagcttt
     6481 ataaaaatct tgccgatgat ttaaatgttt gtagcaagcg aggtttggca gaacgcttcg
     6541 actctaccat cggagccgga accgtgctca tgccttttgg tggcaaaaag caactcacgc
     6601 caattcaagc aatggttcaa aaagtttcag ttgagaaaaa gcacacggac aattgctcgg
     6661 ttatgtcttg aggctacaac ccttacatta ccgaggcgag cccctatcat ggcgcatatt
     6721 tagcagttgt tgaatcagtt tgcaaattaa ttgcaacggg tgctaagttc gaagatgtgt
     6781 acttaagctt ccaggaatac tttgagaggc ttgaccgcga atcaagccgt tgaggcaaac
     6841 ctctttcagc tttgcttggt gcatacaaag ctcaagttga attagaagtt gccgcaattg
     6901 gcggaaaaga tagcatgagc gggtctttcg aagacattca cgttcccccg acgcttgtgt
     6961 ccttcgctgt cacaactgac aaagtcaaaa acattgtaag tccggaattc aaaacagcgg
     7021 gaaacaaaat tgttattctg aaacccgaat atgatgaaaa cggccttcca aaaattgata
     7081 gcttaaagaa aaatttcgaa accgtcacaa aacttatgcg cgaaggcaag gttgtttctt
     7141 gttggactcc aggcttcgga ggaattgccg aagcaatttt aaagatgtct cttggaaatg
     7201 attttggatt ttcttttgat gaaaatattt caacggatga actcttcaat tattcctacg
     7261 gatcatttgt gatggaaaca acggcagaca tcaaagacgc aaaacttgtt ggaacaatta
     7321 cagatgataa aactttcaat cttggaaaag atgcaattaa aacttcggag cttttagaaa
     7381 tttacgaaaa caagctcgaa gacatttatt cgtgcaacat taaacacagc aaaaataata
     7441 tcgaagattt cacatatgag gcgaaggaat ggccaaagcc tgcaattaaa gttgcgaagc
     7501 cacgcgtttt aattcctgcc ttccctggaa ctaattgcga atacgatacc gcaaaagcta
     7561 tggaagatgc aggttgcgaa gctaaaattt tcatcgtaaa taatttgaat caagactcgc
     7621 taaaaaagag cattgaagat tttgccgctg aaacaaataa atctcaaatc atttttatcc
     7681 ctggtggatt ctctggcggc gacgaacctg acggaagcgc gaagttcatt acttcgtttt
     7741 ttagaaatgc cgaagttaaa gatgccgtga ctgatttgtt agacgctcgc gaaggcctta
     7801 tggctggagt ttgcaacggc tttcaagcac taattaaact tggtcttgtt ccttacggaa
     7861 aaattgttga gccaaacgaa gacgcgccaa cgctcaccta caacgaaatt cacaggcatc
     7921 aatctcgagt tgttcacacg cgaattgctt cgaataaatc tccttggctt gccaacctaa
     7981 atgccggaga cattattaac gtagtagttt caaacggcga aggccgcttc atcgcaaacg
     8041 aaaaagacat tcaggaactt gcaaagaatg gccaaattgc tactcaatat gttgacctca
     8101 atggaaaggc aacagacgat attcacttca atctaaacaa ttcgatgtgc gctattgaag
     8161 gaattaccag cccggatggc cgagtcttcg gcaagatggg tcacgcagaa cgctacgctc
     8221 ctggacttta caaaaacgtt ccaggaaact tcgactataa aatgttcgaa gccgctgtca
     8281 aatactttaa gtaaaccagg aagaattttg gaacgcgcgc cgctcactag ggcttcgcgg
     8341 aatgtcctcg tgcaggcaag ctgccagcaa gcgcatggcg ctgcagaatc gttcgggtca
     8401 cgcattccaa aattcttcct cgtaatctta atattcgatt tggcgatagt aacgacgaag
     8461 atttagtggg tgttcaagga atagttccat gtaggcatct accctgttgt taactgcacg
     8521 caaaatatga tggcttatgc tgcctctgta tttttcatca attcctggca tgtcatagtc
     8581 cttcgtatca attacaaaat ggttcttatt aaccttgcca agaaacttga cgactcgctc
     8641 tgagagcggg cgttgagcgt cttcgccaac aaaaaccgta acaggagtat cttcgtcaat
     8701 aacttctaac atgccatgga aaaactctgg agcggtaatt gacttcgtgc gaattcacat
     8761 ttgctcttct caataacaca tcgcataaga ataagttgca ccataatgat ttccagctcc
     8821 cacaaaatat ctaggatatg caggattttt cttaatacgc tcaacttcgg acttagcaaa
     8881 attgaagcca agctcatccg ctgcttcttc gatatttgca agaccttcgg caagcgatgc
     8941 ttccatttgt ttgttatact catcgtaatc tggaaaatcg ccgtttttaa acattagata
     9001 attaccaacc atataaaact taagctgctc attttctgga tagcaaatta aataatcttg
     9061 atattctggc tttgtaagtt ctgtgtctgg agtatctaca aaagcaaaaa ttcgagttcc
     9121 ttgagctttt actgccttgc aaaattcaat catctcagct gtggttccag aaaccgttga
     9181 ataaacaact aaagatttat ccgttaactt tttgttacct gtggtcaaat attctgcagc
     9241 attttcaacg tagacaggaa cattagactt gccacgcata taaacttcca actgcattga
     9301 acttgctcaa gtgcctccaa ttcctacaaa gaaaatatta tcaaagccat catcccaaac
     9361 cttgtctaga atttcttcga ttttaggtcg aagcgcaaga gcgccgttaa tgctatcaat
     9421 tttcttttgt ctgtcaaagt ttcgaagcga agggctttcc ttcgactctg cctttaaagc
     9481 ttcgattttt tgctttgatt ctactgaact tgggtgattc ttatccataa ctcctcctta
     9541 ccaagcacca aaatattgga ttgtttctgt ggcggttaaa gcgccaattt tcatagcttc
     9601 atcaatttgg gcggcttcgc caattgcaga caaaaagcct gctatatagc tatctccagc
     9661 acccattgta tcaacaacat tgtcacattt aacaatgccg aattcgtaaa attccttgcc
     9721 gtcgaagcaa atacttccct tctcaccgcg cattgcgata ataagttgcg ggcctttgct
     9781 tcgtaattct ttcattatta agcgtagctc ttcggttgaa gtattatcgt tagagaagaa
     9841 taaataatct gtatgaggaa tggcaacttt gcaagcatcg tcacctggat cagtcgcgca
     9901 atcaaaagca gttttaattc ccctgttatg caaggtttca atttgatctt caaccttgcc
     9961 ccacaaatca caaacaacaa tgtcgtgctt gcaaataaaa tcaatatcat cattgtcaag
    10021 gcgataattt gctagcactc cttcatcata atcgccgagg attcgctcac cgttatcaag
    10081 cttaacttga gtaactgcag tcttgccttc cataacatgc aaatgagaaa tatcaacacc
    10141 acgctcagca atggaggctt tcattaaatc gccaaattta tcattgccaa tgtgtccaat
    10201 gtatgaactt tcgtgcccaa gacgtttgac ataaacagcc atattaacgg ggccaccacc
    10261 gggataggct cgaccccctt caatatcagc atagaaatct atgcagttac ttccaattgc
    10321 agcaagtttc atttacttcg acgctttctt ctttggattg ttctttctgt cccagaagaa
    10381 aaatgcaggc aacccggttc caacagtaac tgccgatgca ataataccag caagtggagc
    10441 ccatgcaaaa gttgaaacaa tcaaagtaag tgtcataaac atcgcaatta caggcattgt
    10501 ataaccaagt ggtgccctgt aagatggatt gtactgactc ttcggttttc tgcgaagaac
    10561 aaatattgtt ccaaaagtac caaagttacg aagcagcgca acaattgtaa agtatcccaa
    10621 aatatcgttt aggttactta caaaaatcaa gaagatagtt agtgcgctgt gcatcataat
    10681 tgcaacataa ggagttttga aattcttgtg aactctgcca aaaatcttga agaaaagccc
    10741 gtcttttgcc attgcatatt caatacgagg ttggcacata atgcaacttg aaacagaacc
    10801 taaaattaca agaataatca taacagcaac aatgttttca gcataaggtc caataactgg
    10861 taacttacca gctaaaagag cgattggtgc agtgctttcg gcaagttcat caattgaaag
    10921 caagccagtt gaaactgttg taagaacagc atataaagaa agaacaataa ttgcagtaat
    10981 aattaaagcc ctggggattg tcttctttgg gtttttgatc tcgcctgaca tatagcaagc
    11041 agcagccatg ccatcatagc ttcacgtcgt tgccgaaact cccgcaagca aagcaacaat
    11101 tccaaggttg ccagttgcag cagcagtgtc aagaggttga ggcgacaaaa ataattcttg
    11161 attaatataa aatgcgccaa aaccaacaat gaacaagaat ggcaaaatct ttacagcagt
    11221 taggattgta ttaataatac caccaccttt tacactacga agatgaatgc acataaatgt
    11281 aaaatcaata gcagccgcta aaaatttctg tccaatatcg gggagaggac aaaataccaa
    11341 cacatgatta acaattgcaa gctccattac agctaccgaa ggcggatcaa ttgcccagta
    11401 actcattcat ccgcataaaa acgctaaagg tcgagagcct gcttcgcgga aataaatgta
    11461 gtgggcacca tcttctgggt aagcacttgc aagttctgcg taacacaaat tagatggaat
    11521 ttgaagtaat ccaccaataa caaatgagag aaccaaaaat aaagcactac ctgaagcaag
    11581 agcaacttct tgaagcgaag aaaaaatacc cgatccaacg gttgcaccaa tacccaatgc
    11641 aacaacagga atcaaagtaa gtcttcgacc aagctctggg ttttgagcag cagcttgctt
    11701 ccccatctcc ttagcttgag cttttgtgat tttctttttt tgagccatat aaaaccccct
    11761 atgtattatt tgaaccaagt tatattaaca catagggggg tgttattatt aactatttag
    11821 agatattttt gttcttttgg attacgtcgt gagctccacc ttcgcgaatg cttgttgacg
    11881 aaactagtgt aataactgca ttcttttgaa gctgcggaat tgtaagagac ccgcaattgc
    11941 acatggttga cttaaccttg gcaagcgaag cacgaacatt atcctgcaaa gcaccagcgt
    12001 atggaacata agaatcgacg ccttcttcga aagaaaggcc cttcttacca cccatgtcat
    12061 aacgttgcca attgcgagca cgagtcgaac cttcgcccca atattctttg taataagttc
    12121 cgccaatatt aacacgctca gaaggactct catcaaacct tgcgaaataa cgtccaagca
    12181 ttacgaagtc cgcgcccatt gcaagtgcaa gtgtaatgtg atggtcataa acgatgccgc
    12241 catctgaaca aattggcaca taaatgcctg tttttttgaa atatttgtcg cgctctttgg
    12301 caacttcgat aagtgcagtt gcctgaccac gtccaatacc tttcgtctca cgagtgatac
    12361 aaattgatcc accaccaatg ccgaccttta taaagtcagc accgcaatta acaagaaagc
    12421 gaaaaccttc ggcgcttaca acattaccag cgccaatctt aactttgttt ttatatttct
    12481 tgcgagtcca ttcgattgtc tttttttgcc aatcggaata accctccgaa gaatcaatac
    12541 acaaaacatc agctccagct tcgacaagag cagggattct gtcct
//
LOCUS       DBNCDC010000021        12322 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_1908_length_12322_cov_5.510801, whole genome shotgun sequence.
ACCESSION   DBNCDC010000021 DBNCDC010000000
VERSION     DBNCDC010000021.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 12322)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12322)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12322
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1908_length_12322_cov_5.510801"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="AC04VQ_02675"
     CDS             <1..936
                     /locus_tag="AC04VQ_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553114.1"
                     /translation="PRTTKVSSGYKTPRQQIEEARRRKNENTESDEVVGTSAFGSRTI
                     HVFGNKYKNTPRARRELDRENDPSHSRTAYKANTNTDRDLLVGDDYKSAKERLESRKR
                     KNMTKRQKLSRKDSYELSDMENPNGSATNKRANGSYNFMNIYQNKGANNAAQSKLMIP
                     VIGAAVIVLIIILICVFFFVGKQHENDKTDVSNLNVVGISDVENPNRDNGENHEENSA
                     SEPKEVEFKYKVKDGYSVYMEIYEGSNSSHPTLAREVKAGETNTFKVTDTLKFVTSKP
                     EGVEIYVDNELVQPQDTKGVGVYNYTVDFKEWLNA"
     gene            990..1895
                     /locus_tag="AC04VQ_02680"
                     /pseudo
     CDS             990..1895
                     /locus_tag="AC04VQ_02680"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
     gene            1977..2273
                     /locus_tag="AC04VQ_02685"
     CDS             1977..2273
                     /locus_tag="AC04VQ_02685"
                     /inference="COORDINATES: protein motif:HMM:NF037386.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553115.1"
                     /translation="MDDQVDATKKYPRTNKLREIADEISLGKLGELVGESFDVLIEGI
                     EEGRYFGRAYFQAPDIDGVVFISEFDDKKINIGDYLNVKILDTEGYDLVGVIND"
     gene            2434..2595
                     /locus_tag="AC04VQ_02690"
     CDS             2434..2595
                     /locus_tag="AC04VQ_02690"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIO9553116.1"
                     /translation="MFVILSLTDSVDGYLARSRNEITDFGKFMDPLADKILVFAALVA
                     LIELHVLPS"
     gene            2809..3375
                     /locus_tag="AC04VQ_02695"
     CDS             2809..3375
                     /locus_tag="AC04VQ_02695"
                     /inference="COORDINATES: protein motif:HMM:NF014517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HIO9553117.1"
                     /translation="MIIALILTVVSMIDYLYNAREVFDSKTSDNGELAREVVELAKAK
                     GVKIGSAESLTGGLVSSSIVKVPGASNVFSGGIVSYMYSVKEKLLDVDPVHLKNYGAV
                     NEVTAKKMAKSAVTKLDCDIAISTTGIAGPDKDEFETEVGVVYIGFANKKDVRAKRFK
                     FSGSRDEIRSLAVEKSLEIFKTLLSDLP"
     gene            3573..4649
                     /gene="recA"
                     /locus_tag="AC04VQ_02700"
                     /pseudo
     CDS             3573..4649
                     /gene="recA"
                     /locus_tag="AC04VQ_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009307028.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
     gene            4717..5079
                     /locus_tag="AC04VQ_02705"
     CDS             4717..5079
                     /locus_tag="AC04VQ_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIO9553118.1"
                     /translation="MVNREKFSIDVYSKAIKKAKDYDIVNDERYAELYAFSKSHACRG
                     TDGIIRHFSQMKIDYESIPSVVEILNEAKERELQVAIGYLKRHPSRSKNAYASHVRKL
                     INRGFSTSLAIRAANYLL"
     gene            5148..6689
                     /gene="rny"
                     /locus_tag="AC04VQ_02710"
     CDS             5148..6689
                     /gene="rny"
                     /locus_tag="AC04VQ_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012798212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIO9553119.1"
                     /translation="MEIIGIIIGVVVALGIGGVAGVLISKRALNQSAQSAADKAQNIL
                     DKAKNDADTIVKQAKLEAKDEKIKIKAEVEEENKDKLREIREGENRNSQREASLDKRA
                     ENLDDREDHLESFQGELDEREVKIADAEKRINDQLEHVSGLTKEQAKEELIANLKDEV
                     TSKSAFIIRQAEEQAKLESDKKAREIISLAIQRCASDHVSEVTVSTIQIPSDEIKGRI
                     IGREGRNIRSFEQITGTNLIIDDSPETVTVSSFDPVRREIAAVTLKKLIADGRIQPAR
                     IEEIHKKVEKEVNQRVQEAGEEAVFETGIHNLSPELVQTLGKLEYRTSYGQSVLKHSL
                     EVAYLAGIMATELGLDPAAAKRAGLLHDIGKAIDHEVEGSHAVIGADLAHKAGENDEI
                     VHAIHAHHNDIEMTTILDILVQSADAISASRPGSRRESLENYIKRLEEIEAIANEQKG
                     VEKTFAIQAGRELRVMVKPEEVDEDKATVMAHDIAAKIEKDVQYPGQVKVVVIRETRA
                     EGLAK"
     gene            6690..7802
                     /locus_tag="AC04VQ_02715"
     CDS             6690..7802
                     /locus_tag="AC04VQ_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIO9553120.1"
                     /translation="MSEQTGTDLKDFVDKHAQTSHLRVEKDLGGGFFRLRPGEAQRRQ
                     AQQDIRSVEDAVIELLRNSRDAGASNIYLATNTEDAFRRILVIDDGSGVPKSHHKTIF
                     EPYVTSKLDTMTTDTWGVHGRGMALYSISMNAEDAHVVKSEVDLGCAIAVKCYTKKLP
                     EKRDQSTFPDFYVNEDKELVVRGPKNILRTACEFAVESRKTCNVFIGSPVEIAATLFF
                     LAQAKKDVKSVFSEPDSNTKVVDLLGYSSDVHDFKKYAAEIGISLSKRTARRIMDGDI
                     NPQASLLERIAKSGVSGSNASEFNISDVTKVHHAKPKVPTIKLDDTDKESLVNAIKQE
                     FPNVADKYYLKSDIDPKVTIKKNKISISFEVSESDN"
     gene            7909..8181
                     /locus_tag="AC04VQ_02720"
     CDS             7909..8181
                     /locus_tag="AC04VQ_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012798214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HIO9553121.1"
                     /translation="METVLLKVSSKSSPANVAGAIAGLIKDGKEVEIQSVGAAAVNQA
                     IKSVAISRGFLSPVGVEISIVPAFATITINEETRTAIRFKVLASKK"
     gene            8197..8661
                     /locus_tag="AC04VQ_02725"
     CDS             8197..8661
                     /locus_tag="AC04VQ_02725"
                     /inference="COORDINATES: protein motif:HMM:NF013112.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553122.1"
                     /translation="MALNFKDINFHIVTFGCQMNKHDSERICGLLESLGATAVDSIDK
                     CDIVIYLTCCVREAADVRLYGQASSIKNIPLRKESPLINRICAIGGCVGQRDGNELID
                     HVGNVDVVFGTHNIGHLPALLKDSIEKRKTVVEVLDKSSGFPTDLPTHREKP"
     gene            8677..9552
                     /locus_tag="AC04VQ_02730"
     CDS             8677..9552
                     /locus_tag="AC04VQ_02730"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HIO9553123.1"
                     /translation="MTIGCNNFCSYCIVPYVRGREKSRTIEDIIEEAKKYKSEGVLEL
                     TLLGQNVNSYGRDLYGNPKFYDLLCKIDELGFARLRFATSHPKDISDDVIKAFGSLKS
                     LQPALHLPVQSGSDRILKEMNRKYTADYYLGLIEKLRSARPDIALSTDIIVGFPGETQ
                     EDFDATYKLVDEVKYHQVFTFIYSKREGTPAAKIQDDTPKDVIQKRFDSLVELVADRA
                     NELNQQDLNSTMDVLFEGTSKKDSSVLVGKSPKNQTVHVDFGNKKASDFTGKILPVKI
                     SEAKTWYLKGELLAV"
     gene            complement(9712..10200)
                     /locus_tag="AC04VQ_02735"
     CDS             complement(9712..10200)
                     /locus_tag="AC04VQ_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIO9553124.1"
                     /translation="MIEPACVSVHAVLVDHPKPKEKILVMGCGTIGLGVVQAIKVVQP
                     DCELWIMERVPTKIDFAMKLGADHVLKGDPLQATADATGGSEVYKGMSEKNRYFFGGF
                     DRVYDCIGGDWSNTTAVRLLAARGTMIKIGHHMCAITYDESPVWWQELKLIGVDAHGM
                     EE"
     gene            complement(10243..10587)
                     /locus_tag="AC04VQ_02740"
                     /pseudo
     CDS             complement(10243..10587)
                     /locus_tag="AC04VQ_02740"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
     gene            complement(10600..10767)
                     /locus_tag="AC04VQ_02745"
     CDS             complement(10600..10767)
                     /locus_tag="AC04VQ_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553125.1"
                     /translation="MKTVYFDKDIPRVVATKGIVKAGSKIKAANKLLYGGLNAVKYAK
                     NVPDPALPADN"
     gene            10851..11036
                     /locus_tag="AC04VQ_02750"
     CDS             10851..11036
                     /locus_tag="AC04VQ_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HIO9553126.1"
                     /translation="MAMIDDIIVLCGPTATGKSDVANSLAAKINGEVVSADSMQIYRG
                     MDIGTGKIPANKQSVKH"
     gene            11046..11786
                     /gene="miaA"
                     /locus_tag="AC04VQ_02755"
                     /pseudo
     CDS             11046..11786
                     /gene="miaA"
                     /locus_tag="AC04VQ_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013845.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
     gene            complement(11758..>12322)
                     /gene="lexA"
                     /locus_tag="AC04VQ_02760"
     CDS             complement(11758..>12322)
                     /gene="lexA"
                     /locus_tag="AC04VQ_02760"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIO9553127.1"
                     /translation="QIIKDFIADHDYSPTARDIMKRTKLKSVSSVHAYIIELENKGLI
                     TRENNKSRSIKLSGSAGTSPRVDLPVVGDVAAGTPILAEQNISENVTLPADLAGRGNF
                     ILKVRGNSMIDAGIYDGDKVIVRQQKTIKNGEIAVVLLGDEATVKRFYKEKDHFRLQP
                     ENKRMKPIIVDECELCGKVIGLYRSIR"
BASE COUNT         3870 a         2195 c         2703 g         3554 t
ORIGIN      
        1 cctagaacta cgaaagttag ttcaggatac aaaactccaa gacagcaaat agaagaggcg
       61 cgtcgtagaa aaaatgagaa taccgaaagt gacgaagtgg taggaacttc ggcctttggt
      121 tcaagaacta ttcatgtgtt tggcaacaaa tataaaaata cacctcgtgc aagacgtgaa
      181 cttgacagag aaaatgatcc ttcgcatagc agaacagctt acaaagctaa cactaacacg
      241 gatagagatt tacttgttgg cgatgattat aaatctgcaa aagagcgtct tgaaagccga
      301 aaaagaaaga acatgaccaa gcgccaaaaa ctctcgcgca aggattctta tgagcttagc
      361 gatatggaaa atccaaacgg aagtgctact aataaacgcg ctaatggctc atacaatttt
      421 atgaacatct atcaaaataa aggcgccaat aatgctgctc aatccaaatt gatgattcct
      481 gtcattggag ctgctgttat agtcttgatt attattttga tttgtgtgtt tttcttcgtt
      541 ggtaagcaac atgaaaatga taaaaccgat gtttcaaatc taaatgttgt gggcatttct
      601 gatgttgaaa atccaaatag agacaatggc gaaaatcatg aagaaaattc tgccagcgag
      661 cctaaagaag ttgagtttaa gtacaaggta aaagacggct atagtgttta tatggaaatt
      721 tacgaaggca gcaatagctc tcatcctaca cttgctcgcg aagttaaggc gggtgagacc
      781 aacacattta aagtcactga cacgcttaag tttgtaactt ccaagcctga aggtgttgag
      841 atttatgttg acaacgaact tgttcaacct caagacacaa agggcgttgg cgtttataac
      901 tacactgttg attttaaaga atggctaaac gcttgaaaga ccaagcacaa aacctcgagc
      961 taaccatgga aaaatcaaat actaaaaaaa tgtcgctaaa tgtatttttc gacactttag
     1021 gttgtgcgaa aaacgaagtt gataccttta agatggaggg ccttgttaaa ggcgcagggc
     1081 ataatattgt cgaagacgca tcgcttgccg acgtcattat attaaacact tgtgctttta
     1141 tcgaagccgc cacgcaagag agcatcgata cattttttga atatagaaat tattataaag
     1201 acaaaaagat tattgtttgt ggatgtttgg tgtcaagata taaggacgaa cttaaaaaat
     1261 ctttaaatga ggcagacgct tttatttcat gtgacgatga agaaaagatt tgtgagctaa
     1321 ttacaaacct taatggtaaa ccttcaaaca ataacgaaac aaaggaggct tcgtcttcgg
     1381 cgtttggata tgtaaaaatt tctgagggtt gcaacaggag atgtagttat tgcacgatac
     1441 cttttattcg tggaaaatat aaaagttcaa gctatgatga aattgtaaaa gatgtcgaag
     1501 agcagcgcga cctcggagct aaagagatag ttttggttgc tcaagattgt ggagtgtgag
     1561 gctgtgatct tgatggcaaa aaccttgctt ggctcactaa taatctttgt gaagcttttg
     1621 cagagctaaa ctttaggttg ctttatgttt atcctgaaac tattgacgat gatttgataa
     1681 atgcttttaa aaacaacaag aatttaatta actatatcga tatgccaatg cagcattgca
     1741 acgctaaggt tttaaaagct atgggcaggc gtggagatag agattcgttt agattgcttg
     1801 tcaataaatt acgaagcagt attcccggtg tcgctttaag atcgactttt atatgtggtt
     1861 ttcctggcga gagcgacgac gacttcgatg agctttgcga ttttattgaa gagataaagc
     1921 ttgattatgc tggatgtttt gaatattctt gcgaagaagg cacaaaagca gctcttatgg
     1981 atgatcaagt tgacgcaacg aaaaaatatc caaggacaaa taaacttcgt gagattgctg
     2041 atgagataag tcttgggaag ttgggcgagc ttgtgggcga gagcttcgat gttttaattg
     2101 agggcatcga agaggggcgt tattttggtc gtgcttattt tcaagctcct gatatagatg
     2161 gtgttgtttt tataagtgag ttcgacgata aaaaaataaa cattggagat tatctaaatg
     2221 ttaaaatatt agacaccgaa ggttatgatt tagtaggagt tatcaatgac taaggaaagt
     2281 aagaaaaata cgatttgaac ggcagcaaac attgtcacaa ttattcgcat attgtttgtt
     2341 cctgtttttg ttgttgcgct tatttctcct tggccttcgt ggattgacaa tagtggtggt
     2401 cttgatttct taaagccatg agttgcgctt gtaatttttg ttattttgtc tcttaccgat
     2461 agcgttgatg gctatttggc aagaagcaga aatgaaatta ccgattttgg aaaattcatg
     2521 gatcctcttg cagataagat tttagttttt gctgctcttg ttgctttgat agaacttcat
     2581 gttttgcctt cgtgagttgt tttaattatt ttaatgcgtg aatttatcgt tgctggcgtg
     2641 cgaatgctcg ctgcttcaaa aggcatggtt attgctgcaa gttgatatgg caaagctaaa
     2701 acggtaactc agctaatagc aattattttg ttcattttga aggaatcgct tgcatcaagt
     2761 ggggctcagg acatcgccaa tccgttatat attgtcgctt ggatcgtgat gattattgcg
     2821 ctcattttaa cggttgtttc gatgattgat tatttataca acgcccgcga ggtttttgat
     2881 tcgaagacaa gcgacaacgg cgaacttgct cgcgaagttg ttgagcttgc aaaagctaag
     2941 ggcgtaaaaa ttggcagcgc tgaaagctta actggtgggc ttgtttcttc ttcgatagtt
     3001 aaggtgccag gagccagtaa tgtattttct ggtggaattg tcagctatat gtattctgtg
     3061 aaagaaaagt tgcttgatgt tgatccagtt catcttaaaa attatggtgc tgttaacgaa
     3121 gtgaccgcga aaaaaatggc gaagtctgct gtaacaaaac tcgattgtga cattgccata
     3181 agcacaacgg gaatagctgg acctgacaaa gatgaatttg aaacagaggt tggtgttgta
     3241 tatattggat ttgctaacaa aaaagatgtt agggcaaaaa gatttaaatt tagtggatcc
     3301 agagatgaaa ttcgatcatt ggccgtagaa aaatctttag aaatatttaa aacgcttctc
     3361 tcagatttac cttaaattat ttttctttaa taattattat ttttatttaa aacttttata
     3421 ttatttttta ataaaaagcg ctggtaatga attataatta atttgcgatt ttgcttgaaa
     3481 gtataagcaa tttttaataa tattcgcctt cctatataag ctagcgtata ggcaaaaatt
     3541 taaaaattgt ttatatgaag ggagatttaa ggatggaaga aaataaagaa gaattgttaa
     3601 aatctaccac cgatcaaatc attaaaaaat ttggtaaagg ctcaattatg aaaatgggcg
     3661 aaggctcaac tacttttgat atgggcgtaa taccaacagg agctctttct ttggacgccg
     3721 cattaggcat tggaggtgtt ccaagaggca gaatcgtaga gatcttcgga cccgaatcga
     3781 gtggaaaaac tactttgggt cttcagattg ttgccgaagc tcaagcccaa ggtggcattg
     3841 cggcctatat tgatgcagaa catgcacttg accccgttta tgccgcaaag cttggtgtag
     3901 atgttgacaa cttgctaatt tctcagccgg atactggcga gcaaggcctt gaaatttgtg
     3961 atatgttggt tcgaagtaaa tcaatttctg ttgttgtaat cgactctgta gctgctcttg
     4021 ttccaaaaag cgaaattgag ggagagatgg gcgaagttgg cgttgggtct cttgcaagat
     4081 tgatgagtca tgctcttcgt aagctaaccg tttcagttgc tgaatctaat acaacttgta
     4141 tttttatcaa ccaattgcgt gaaaaaattg gtgttatgtt tggtagtcca gagacaactc
     4201 ctggtggacg cgcattgaag ttttcggctt cggtcagact tgatattaga aggcgtgaat
     4261 ggattaaaga aggcagtgaa catatgggct cacgcgttcg agttaaagtt gttaaaaata
     4321 aagttgctcc accttttagg caggctgaat ttgatcttat gtttggcgaa ggtgtctcta
     4381 aggaaggctg tattttagat atggcagcag atgctggaat tgttgataag agcggcgcat
     4441 gatttaccta caaggaagaa aaacttggac agggtagaga agccgcaaaa gagacactga
     4501 aggcaaatat taaacttcga gacgaaattg aaaagcaagt tcgcgatgcc ttcggctttc
     4561 cgccgtcaaa caatgtcaag ccaaaagtaa agcctaaaga gcaaaaggct aaagatgata
     4621 aaaccaacga cgaaaaacga ggtaaataac tgttttcggc gtatcgttat actaactaat
     4681 tatcgtgaaa gatgcgaaac tgagctatat gatcgcttgg ttaacagaga aaaattcagt
     4741 atagatgttt attcgaaagc tatcaaaaaa gcaaaagatt atgacattgt taatgatgag
     4801 cgatatgccg aactctacgc ttttagcaaa tctcatgctt gcaggggaac tgatggaata
     4861 ataagacatt ttagccagat gaagattgac tacgagtcta taccttcggt agttgaaatt
     4921 ctcaacgagg ctaaagagcg cgaacttcaa gtagcgatag ggtatttgaa acgtcatcca
     4981 tcaagatcaa aaaatgctta tgcgtcgcat gttagaaaac ttatcaacag aggtttctcg
     5041 acaagtttag caattagggc tgcaaattat ttgttataat atctaaccgt actttgaaat
     5101 aataaatcta taaacgaaga ttaaacactg aatattggag gttagatatg gaaattatag
     5161 gtattattat tggtgttgtt gttgccttag gaataggggg agtagctgga gtcctcatat
     5221 ctaaaagagc attaaatcaa tcagcacaaa gtgctgcaga taaagcccaa aatattttag
     5281 acaaggctaa aaatgatgca gacacaattg ttaaacaggc taaattagaa gccaaagacg
     5341 aaaaaattaa aattaaggct gaggtcgaag aagaaaacaa agataagctt cgagagattc
     5401 gtgaaggcga aaatagaaat tctcaaagag aagcaagtct tgataaaaga gccgaaaact
     5461 tagacgatcg cgaagatcat cttgagtctt ttcaaggtga gcttgatgaa cgcgaagtta
     5521 agattgctga tgctgaaaag cgcatcaatg atcaacttga gcatgttagc ggcttaacaa
     5581 aagagcaggc caaagaagaa cttatcgcta atttaaaaga cgaagttact tcgaaatctg
     5641 cttttataat tcgacaagcc gaagagcaag ccaagcttga atctgacaaa aaagcaagag
     5701 aaattatcag tttggccatc cagcgttgcg catctgatca tgtttctgaa gttaccgtct
     5761 cgacaattca gatcccgtcc gatgaaatta agggtaggat tattggtcgt gaaggtagaa
     5821 acattcggtc atttgagcaa attacaggca caaatttgat aattgacgat tctcccgaga
     5881 ctgttacagt gtcaagtttt gaccctgttc gtcgtgagat tgctgctgtt actttaaaga
     5941 agctaattgc tgatggtcga attcagcctg caagaattga agaaattcac aaaaaggttg
     6001 aaaaagaagt taatcaacgt gttcaagaag ctggcgagga agctgttttt gaaacaggaa
     6061 tccataattt aagtcctgag cttgttcaaa ctttaggtaa gcttgaatac agaacttcct
     6121 acggtcaaag cgttttgaag cactctttag aggttgctta tctggctggt attatggcaa
     6181 ctgagcttgg tttagaccct gccgcagcaa aaagagctgg tcttttacat gatattggta
     6241 aggccattga tcatgaagtt gaaggttcgc atgctgttat tggtgcagac ttagctcata
     6301 aggctggcga aaatgatgag attgttcatg caatccatgc tcatcacaac gatattgaga
     6361 tgacaacaat tcttgatatt ttggtccagt ctgcggacgc aatttctgct tcaagacccg
     6421 gttcacgtcg cgaatctttg gaaaactata tcaagcgtct tgaagaaatt gaggcaattg
     6481 ctaacgagca aaaaggcgtc gagaagacat ttgcaatcca ggcagggcga gagcttcgtg
     6541 ttatggttaa acctgaagag gttgacgaag acaaggcaac cgtaatggca catgatattg
     6601 cagcgaagat cgaaaaagac gttcaatatc ctggtcaagt taaggttgtt gttattcgtg
     6661 aaacaagagc agaaggcctc gcaaaataaa tgtctgagca aactggcaca gacttaaaag
     6721 attttgtaga taagcatgca caaacaagcc atctcagagt tgaaaaagac ctcggaggtg
     6781 gcttttttcg tcttcgtccc ggcgaagcgc agcgcaggca agctcagcag gatattcgaa
     6841 gcgttgaaga cgccgttata gaattgcttc gtaattctcg tgatgcgggc gctagcaaca
     6901 tttatcttgc gacaaatacc gaagacgcct ttaggcgtat tctagttata gatgatggtt
     6961 ctggtgtccc gaaatcgcat cataaaacca tctttgagcc ttatgttacg tcaaaactag
     7021 acacaatgac aactgacaca tggggcgtcc atggtcgcgg aatggcctta tattcaatat
     7081 ctatgaacgc ggaagatgct catgttgtta aatcggaagt tgatcttgga tgcgctattg
     7141 cagttaaatg ttatacgaag aaattacctg aaaagagaga ccaatctaca tttccagatt
     7201 tctatgtaaa cgaagacaag gaactggttg ttaggggacc gaagaatatt ttgcgaactg
     7261 cttgcgaatt tgcagttgag tctcgtaaaa cgtgcaacgt ttttattggc agtccagttg
     7321 agattgccgc tactttgttc tttttagctc aagctaaaaa ggacgttaaa tcagttttta
     7381 gtgagcctga cagcaacact aaagttgtag atttattagg ctattcgtcc gatgtccatg
     7441 acttcaaaaa gtatgcagcc gagattggca tctcgctttc aaagcgcacc gcaagacgta
     7501 taatggacgg cgacattaat cctcaggctt cgctcctcga gcgaattgct aagagcgggg
     7561 tttccggaag caacgcttcc gaattcaaca tatcagatgt tacaaaagtc caccacgcca
     7621 aaccgaaggt tccaactatt aaactcgatg atactgataa ggaatcatta gttaatgcta
     7681 tcaaacaaga atttccaaac gtagctgata agtactatct taagagcgat attgatccta
     7741 aagttacaat caagaaaaat aaaatctcaa tatcattcga agtttcagaa tcagacaatt
     7801 aagcgcttgc gctagcatct tgtgttataa tacggctccc aataattatg aatatagaaa
     7861 ttactgaact ttaattattg caatgtaaac cgaacaaatg gaacaaaaat ggaaactgta
     7921 ttattgaaag tgtcatctaa atcttcgcca gcaaatgtag ctggtgcaat tgccggtcta
     7981 atcaaggacg gcaaggaagt agaaatccaa tcagttggag cagcagcggt caaccaggcc
     8041 attaagtctg ttgcgatctc tcgtggattt ttatctcctg ttggtgttga aatcagcatt
     8101 gtgcctgcat ttgcaacaat tacaatcaac gaagagacac gcacagcaat tagattcaag
     8161 gttttagctt ctaaaaaata attttgcgag gctaccttgg cactcaactt taaagatatc
     8221 aattttcata ttgttacttt cggctgccaa atgaacaagc atgacagcga gaggatttgt
     8281 ggcttactgg aatctcttgg agcaactgcg gtagacagca ttgataaatg cgatatcgtc
     8341 atttatttga catgttgtgt tcgcgaagct gcagacgttc gtctttatgg gcaggcaagt
     8401 tctattaaaa atatcccact gcgaaaagaa tcaccgctaa ttaatagaat ttgcgcaatt
     8461 ggtggatgcg ttggccagcg tgatggcaat gaactaattg atcacgttgg aaatgttgac
     8521 gtggtcttcg gaacacataa tatagggcac ctgccagctc ttttaaagga ttcaattgaa
     8581 aagcgcaaga cagttgttga ggttttagac aaatcgagtg gctttccaac ggatcttcca
     8641 acgcatagag aaaagccttg agctgcttgg cttccaatta caattggttg caataatttt
     8701 tgctcttatt gcattgttcc gtatgttcga ggtcgagaaa aatctcgcac gatagaagac
     8761 attattgaag aagcgaagaa atacaaatcc gaaggtgttt tagagttgac tctacttggg
     8821 caaaatgtaa attcctacgg cagggatttg tacggcaatc ctaagtttta tgatttactt
     8881 tgcaagattg atgagcttgg atttgctcgg cttcgcttcg ctacgagcca tccgaaagat
     8941 atttcagacg atgtaattaa ggcttttgga agcttgaaga gtttacaacc ggctttgcat
     9001 ttgccagtcc aaagtggcag tgacagaatc ctaaaagaga tgaacaggaa atatacggct
     9061 gattattatt taggattgat cgaaaaactc cgaagcgcac gccccgacat tgcactttca
     9121 actgatatta ttgttggatt tccgggtgag acgcaagagg acttcgatgc aacatataag
     9181 ctggtagacg aagtaaaata ccaccaagtt tttacattta tatactcgaa acgcgaaggt
     9241 acgcctgcgg cgaaaattca agacgatacg ccaaaagatg taattcaaaa acgatttgat
     9301 agtctagttg agcttgttgc tgatcgcgcg aatgagctaa accaacaaga cttaaattca
     9361 acaatggatg ttttatttga aggaacttcg aaaaaagact ctagcgtttt agtagggaag
     9421 tctccgaaga atcaaaccgt tcatgttgat tttggcaata aaaaagcctc ggactttaca
     9481 ggaaaaatcc ttccagtaaa aatatccgag gccaaaactt ggtatctaaa aggcgaatta
     9541 ctcgcagtct agaacaatct taataacatc cggtggattg agaagcgcaa gcttcatcgc
     9601 ttctttgtaa tcgtctagct taaagtggtt tgttacgaaa cccttaaccg aatattttcc
     9661 ttccttgatt catttttgaa ccaagtcaaa tgtatacatt ttttcgcctt ctcattcttc
     9721 cataccatga gcgtcaacac caataagttt aagttcctgc caccaaacag gagattcgtc
     9781 ataagtaatt gcgcacatat gatgcccgat tttaatcatc gtgccgcgag cagcaagtag
     9841 gcgaacagct gttgtgttag accaatctcc accaatgcag tcataaaccc tgtcaaagcc
     9901 accaaagaaa taacgattct tttcgctcat tcctttgtag acctcagatc ctcctgtggc
     9961 atcagctgta gcttgtagcg ggtctccctt caagacgtgg tctgcaccaa gtttcatagc
    10021 gaagtcaatc tttgttggaa cgcgttccat aatccaaagt tcgcaatctg gttgaacaac
    10081 tttaatagct tgaacaacgc caagaccaat agttccacaa cccattacaa gaattttttc
    10141 ctttggcttt ggatgatcaa ccaaaacagc atggacagat acacaagccg gttcaatcat
    10201 gcatgcgtct tcgttgtcaa tttcgtcaat aattttataa agttgttgct caggagcaat
    10261 aaaaatatca ccccagccag cacctatcca ttcaagatca tattttggat caacaccgta
    10321 atttgtgcag aaattataat ttccttcgcg acaatgacgg caacgaggca aatctttatt
    10381 gtcgcaacca gccatataag cgcgaattcc aacacggtct ccaagcttaa aatctttaac
    10441 agcgcttcca acttcgatta cttcaccaac attttcatgt ccaaggtaag ttttcttgct
    10501 tgccggaatt ggttcaaatg cagaatttgt gccagttgtt gccttaaaaa agctcatgtc
    10561 ggtgccgcat aaaccgcagg cgatatttcg aaccttaact caattatctg ctggaagagc
    10621 ggggtccgga acgtttttcg catacttaac tgcgtttaag cctccataaa gaagcttgtt
    10681 tgcagccttg attttcgagc cagctttaac aattccttta gttgctacaa cgcgcgggat
    10741 atctttgtca aaatagacag ttttcatagt gatctccttt attgatagat tagaatattc
    10801 gctcaacaac tttattttag cacaaagttt gtgataacat agcaaaagag atggcgatga
    10861 ttgatgacat aattgttctt tgtgggccca cggctactgg caaaagcgat gttgcaaatt
    10921 cgcttgctgc aaaaattaat ggggaagtgg tcagcgctga ttctatgcaa atttatcgag
    10981 gtatggatat tggaactggc aaaatacctg ctaacaaaca aagtgtgaaa cattgaggtc
    11041 ttgatatcgt taatccaagc gaagcttttt cagctgcttt atttcaagat tattcgcgcg
    11101 acgcttttga caaaattttc tccgaagaca agacgccaat tctttgtggc ggaaccggct
    11161 tttatattag agccgcaatt gacgattacg attttccaaa aggcgagcaa gttgataatc
    11221 cgcttcgtga gaaatatcaa aaaattgccg atgaaaaagg cgcgcatgag ttgtgaaaaa
    11281 ttctacaaga gaaagacccc gaaagcgcaa atttaattca tgagaataat gttgttagag
    11341 ttgttcgcgc cttagagatt tttgaagaag gtaaatctta tgctgagcaa gttgaaaatt
    11401 tgcaaaagct accacaaaaa tataatgcga aattttttgg acttaaagta gatcctgaaa
    11461 ttttgcgtga gcgtatagat gctcgtgttg acaaaatgtt tgaggcaggt cttgttgacg
    11521 aggttaaagg tctgcttaat aatgggttgc gtgaggcctt gacttcggcg gccgcaattg
    11581 gttacaaaga aattgttgaa tatttagatg gaaatgtcac actcgacgaa gcggctttgc
    11641 aaatcaaaac cgctacaaga caatatgcga agcgtcagcg tacttggttt aacaaggaca
    11701 agcgaattaa ttggattgat tataacgatg cagatttaga ttcggcggtt gcgcaaatta
    11761 tcgaatgctg cggtaaagac caataacttt tccgcaaagt tcgcattcat caacaatgat
    11821 aggtttcatg cgtttgtttt cgggctgcaa gcggaagtgg tctttttctt tgtagaagcg
    11881 cttgacggta gcttcgtcac caagcaaaac tacagcgatt tcgccatttt tgatggtttt
    11941 ttgttgacgc acgataactt tgtcgccgtc ataaattccc gcgtcaatca tggaatttcc
    12001 gcgaactttc aagatgaaat ttccacgtcc agcaaggtct gccgggagag tgacgttttc
    12061 ggaaatattt tgctctgcca aaattggagt gccagcggcc acatcgccaa caacaggtaa
    12121 gtcaactcgt ggggaagttc cagcgcttcc gcttaacttg attgaacgag atttgttgtt
    12181 ttcgcgagta attagtcctt tattttcaag ctctataata tatgcatgaa ccgaagatac
    12241 agacttgagc ttcgtgcgct tcatgatgtc acgagccgtt ggactgtaat cgtgatctgc
    12301 tataaagtct ttgataatct gt
//
LOCUS       DBNCDC010000022        12278 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_1922_length_12278_cov_8.603289, whole genome shotgun sequence.
ACCESSION   DBNCDC010000022 DBNCDC010000000
VERSION     DBNCDC010000022.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 12278)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12278)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12278
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1922_length_12278_cov_8.603289"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="AC04VQ_02765"
     CDS             <1..962
                     /locus_tag="AC04VQ_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIO9553128.1"
                     /translation="IMAEKKQKEINDMTNKELFKLMSGPSQLKRKNAAKVLTDRVKEN
                     PEEFKKNIPDFVDALSRPEAQTRWLALDCLTHLVPVDAKACKDAIEGAEEALFDDKTS
                     LIRESAVRFLSRYGASSMQRSKEVWPLIKEALQQFHGEIEYDKILNRINFFAAGKLDA
                     GVKKDLRATAKDIQKSVGGPTKSRLERILLSTQGKTLKVPTKKAPAKKAVAKKPEAKT
                     SSTKKATTKNKDVKKKVEAKKKDAKKKAVKKAVKTAVAKPKAKTKVGKKVEKKKKNIA
                     KKVVKKAVKKEVKKAVKKTVKKAVKKTAKKPVKKSVKKTAKKK"
     gene            974..1267
                     /locus_tag="AC04VQ_02770"
     CDS             974..1267
                     /locus_tag="AC04VQ_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553129.1"
                     /translation="MAEKKSGVDSLIDALKASGAPVDDKGQTSSSAKSDTKASEAKGN
                     SKQAQEEQPQVGGLGDIPKAILDMPFMDRFKNMNKKGKIITAIVVLVILGIAY"
     gene            1310..2968
                     /locus_tag="AC04VQ_02775"
     CDS             1310..2968
                     /locus_tag="AC04VQ_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006361822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal sequence"
                     /protein_id="HIO9553130.1"
                     /translation="MIFLILFMLPLFLFWRSRRFKYMPADVEKEKRDKKKTSKAKAAS
                     SAKKEDEKLSKEILTDENEKKGIFYKRLCWIPLGVAGLGLLGMLLSASFFPGNAYKYA
                     DILKTDNLDFETDIQEVNYSQIPVIDRDTARVLGTKEMGTIPDYVSQFEISNLYSQIN
                     YQGKPVRVSCLMYADLFKWLTNREAGIPAYCLIDMATQDAEIKRVSDVDGTEGQGIKY
                     SESEPLARNIERYIQLKYPFEMFDEFSFQIDEDGHPWWICPIKVKTIGLFGGKTIAGV
                     VMCDAVTGECTRMDVNECPEWTDRVYPSDLLLEQYNWSGKYKSGWLNSWLGQSGVVKT
                     TPGTISSSSSSTTTNKERALGYNYIAKDDDVWVYTGVTSATSDSSIVGFILINTRTAE
                     SHFYSIAGATEVSAMESAEGQVQHLRYTAAFPILINVSNVPTYFIALKDAAGLVKKFA
                     MIDIQRYQNVATGDSVAECQKNYQALLTREGIDMTGGGLSGNQITGTIANYMTAVIDS
                     NTHVYFTIAGDPRIFDAALPGMMQIVRYKVGDAISFYASESGET"
     gene            2990..4342
                     /gene="asnS"
                     /locus_tag="AC04VQ_02780"
                     /pseudo
     CDS             2990..4342
                     /gene="asnS"
                     /locus_tag="AC04VQ_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012379.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
     gene            complement(4339..4482)
                     /locus_tag="AC04VQ_02785"
     CDS             complement(4339..4482)
                     /locus_tag="AC04VQ_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553131.1"
                     /translation="MPEGKDVLKKAGLEGNYEGVGNLAIGYPAGEKRSDPDINPDYIS
                     YIN"
     gene            complement(4510..4863)
                     /locus_tag="AC04VQ_02790"
     CDS             complement(4510..4863)
                     /locus_tag="AC04VQ_02790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIO9553132.1"
                     /translation="MNTLEAINSRRSCKNFDPEKPVPEDIIQKICEAGTKAASGMNKQ
                     AAKIIVVNNKEIRDLISKKNAEIFGRGAVDPFYGAPTIICVVANKDVHTYVYDGSLVM
                     GNMMLAAEELGIGSC"
     gene            complement(4879..6252)
                     /gene="tsaD"
                     /locus_tag="AC04VQ_02795"
                     /pseudo
     CDS             complement(4879..6252)
                     /gene="tsaD"
                     /locus_tag="AC04VQ_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013012.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            complement(6316..6489)
                     /locus_tag="AC04VQ_02800"
     CDS             complement(6316..6489)
                     /locus_tag="AC04VQ_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553133.1"
                     /translation="MSDAEESEREKLADISKKNLNTGVQNLEGENKNFEPLDAKDIPQ
                     IVELEKQLMGTDA"
     gene            complement(6574..6783)
                     /locus_tag="AC04VQ_02805"
     CDS             complement(6574..6783)
                     /locus_tag="AC04VQ_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553134.1"
                     /translation="MRHEIYPLLCELSEEGVKRLSPDKVLKAQASADEAFELSTKVDN
                     LTIVGDALHKYFDLFEGLNIGNQEL"
     gene            complement(6835..7155)
                     /gene="tsaB"
                     /locus_tag="AC04VQ_02810"
     CDS             complement(6835..7155)
                     /gene="tsaB"
                     /locus_tag="AC04VQ_02810"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIO9553135.1"
                     /translation="MSDNKYILAFDTANESISIAIANLDDFSLCASFCGEAHRASNTM
                     LLPEVEKLFKKSKVSKSDIACVAAGRGPGSFTGVRIALATAKGIATALQIPLIGFNTL
                     EAVA"
     gene            7192..7899
                     /locus_tag="AC04VQ_02815"
     CDS             7192..7899
                     /locus_tag="AC04VQ_02815"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIO9553136.1"
                     /translation="MMILAVDIGNTQTCVGAFENDSNDLKSLWRFSTPEKITVDEATS
                     DFMQIIDQSEFSVEDVSAVCISCVVPSLTVFYTKAINAFIQKRNLDVKFVVCNAKNSE
                     KLSKSLYNCKYAHPNEVGGDIIATCVAARVLYDMPCAIIDFGTATNINVINSKGEFVG
                     AIISPGLKTSLNALVADAGALSDIEIKVPSKLLGNSTPELIQSGSIIGEAARADGFID
                     RIEEDLGEKVNVICTGG"
     gene            8090..9883
                     /gene="uvrC"
                     /locus_tag="AC04VQ_02820"
                     /pseudo
     CDS             8090..9883
                     /gene="uvrC"
                     /locus_tag="AC04VQ_02820"
                     /inference="COORDINATES: protein motif:HMM:NF020048.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
     gene            10062..10949
                     /gene="rapZ"
                     /locus_tag="AC04VQ_02825"
                     /pseudo
     CDS             10062..10949
                     /gene="rapZ"
                     /locus_tag="AC04VQ_02825"
                     /inference="COORDINATES: protein motif:HMM:NF046811.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
     gene            10975..11928
                     /gene="whiA"
                     /locus_tag="AC04VQ_02830"
     CDS             10975..11928
                     /gene="whiA"
                     /locus_tag="AC04VQ_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HIO9553137.1"
                     /translation="MSFTTDVKDELSRVEPECKTCSKAMLSALVKSHGTLNILGKGNF
                     TVRFATDNAWIARFALENFKVLYNLETSLTYRRSVLHNTRNYLIELNSGPNLQKALID
                     MGILQAPKKDKTKGVASFSHGVKKDFKDKDCCAASYMRGAFLGSGYVSEPSGDFHFEI
                     SVDRKSQAIDMQKILAKKDIKSGVCPRRNSYLLYIKSGQGIADFLAFTGAHKCALKLE
                     STRVSKQIANQINRQTNAEIANSKRTVEAAYNQIVMIKRVAKHYGLENLPPALREFMA
                     LRVKYHDVSLSELGTLADPPISKSAINGRVRRLEHLANTIK"
     gene            11977..>12278
                     /locus_tag="AC04VQ_02835"
     CDS             11977..>12278
                     /locus_tag="AC04VQ_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIO9553138.1"
                     /translation="MTAKYDAVVCGSGNSGLTASLTLAAAGKKVLLIEQHNLPGGSAS
                     SFRRGRFELEPSLHELGEVGPKNNPGAVRKMFDQLGVKVDWIEIPDLFRVVSTWTD"
BASE COUNT         3707 a         2186 c         2629 g         3756 t
ORIGIN      
        1 taatcatggc agaaaaaaaa caaaaagaaa ttaatgacat gacaaataag gaattgttca
       61 agttgatgtc tggtccttcg cagctaaaac gtaaaaatgc tgcaaaggtt ttaactgatc
      121 gtgttaaaga aaatccagaa gaatttaaga agaacattcc tgactttgtt gatgcattgt
      181 ccaggccaga ggctcaaact cgttggcttg cacttgattg tttaactcat ttggttcctg
      241 ttgatgcaaa ggcttgcaaa gatgctatcg aaggtgccga agaggctttg tttgacgata
      301 aaacttcgct tattcgagag agtgctgtta gatttttgag ccgttatggt gcaagttcaa
      361 tgcagcgttc taaagaagtt tggccattga ttaaagaggc tcttcaacaa tttcatggtg
      421 agattgaata cgataaaatc ttaaaccgaa ttaatttttt cgctgctgga aaacttgatg
      481 caggtgttaa gaaagatctt cgtgctactg ctaaagatat tcaaaagagc gttggtggtc
      541 ctacgaagtc aaggcttgag cgaattttac tttctactca aggtaagact ttgaaagttc
      601 caacaaagaa agcacctgcg aaaaaggctg ttgccaaaaa gcctgaggct aaaacttcgt
      661 caactaaaaa agctacaact aagaataaag atgttaagaa aaaggttgaa gcaaagaaga
      721 aggacgctaa gaaaaaggca gttaaaaagg ctgttaagac tgctgtagca aaaccaaaag
      781 ctaaaacaaa agttggtaaa aaagttgaga agaaaaagaa aaatatcgca aagaaagttg
      841 taaagaaggc agttaaaaaa gaagttaaaa aggctgttaa aaaaacagtt aaaaaagctg
      901 taaagaagac agctaaaaag cctgtaaaaa aatcagttaa gaaaactgca aagaaaaaat
      961 agaaaggtag attatggctg agaaaaaatc tggtgttgat tcacttattg atgctctaaa
     1021 ggcatcgggg gctcctgttg atgacaaagg tcaaacaagc tcatcggcaa aatctgatac
     1081 aaaagctagt gaagcaaaag gaaattctaa gcaagctcaa gaagagcaac cacaagtagg
     1141 tggtttagga gacattccaa aggcaatttt agatatgcct ttcatggatc gctttaaaaa
     1201 catgaacaag aaaggcaaaa ttataactgc aattgttgtt cttgttattt taggaattgc
     1261 ttattgatgg tttcatccac caattaatat tcaaagcgaa gagacttggt tgatcttctt
     1321 aattttgttt atgcttccgc tatttttgtt ctggcgttct cgtagattta aatatatgcc
     1381 agcggacgtt gagaaggaaa agcgcgataa gaaaaaaact tctaaggcta aggctgcaag
     1441 ttctgcaaag aaagaagacg aaaaactttc taaagaaatt ttaactgacg aaaatgaaaa
     1501 gaagggaatt ttttacaagc gcctttgctg gattcctctt ggagttgcgg gattaggtct
     1561 tcttggaatg cttcttagtg caagcttttt ccctggtaat gcttataaat atgcagatat
     1621 cttaaagacg gataaccttg acttcgaaac tgatattcaa gaagttaact attctcaaat
     1681 tcctgttatt gacagagaca ctgctcgtgt tttgggaaca aaagagatgg gtactattcc
     1741 tgattatgtt tctcagtttg aaatttcgaa tctttatagt caaattaact accaaggcaa
     1801 gcctgttcgt gtaagttgtt tgatgtatgc tgatctattc aagtggctta cgaatcgcga
     1861 ggctggaatt ccagcttatt gtttaattga tatggcaact caggatgctg aaattaaacg
     1921 tgtttcagat gttgatggaa ccgaaggtca gggcattaaa tattctgaaa gtgaaccttt
     1981 ggcaagaaat attgagcgtt atattcagct taaataccca tttgaaatgt ttgatgaatt
     2041 tagttttcaa attgacgaag atggacaccc ttggtggatt tgcccaatta aggttaagac
     2101 aattggactt ttcggaggaa agacaattgc tggcgttgta atgtgcgatg ctgtaactgg
     2161 tgaatgcact cgcatggatg taaatgaatg tccagagtgg actgaccgtg tttatccttc
     2221 ggacttattg cttgagcaat ataactggag tggtaaatat aagagtggtt ggttaaattc
     2281 ttggcttggt caaagtggtg ttgtaaagac aactccagga accatttcaa gttcttcgtc
     2341 atctactact acaaacaaag aacgtgcttt gggttataac tatattgcaa aagatgacga
     2401 tgtttgggtt tacactggtg taacttctgc cacatctgat tcttcaattg ttggatttat
     2461 tttgattaat actcgtacgg ccgaaagcca cttctattca attgctggag cgaccgaagt
     2521 ttctgcgatg gaatctgccg aaggtcaggt tcaacactta cgttatacgg cagcattccc
     2581 gatacttata aacgtgtcga atgtcccaac ctattttatt gcgcttaaag atgcggcggg
     2641 attggttaag aaattcgcca tgattgatat tcagcgatac caaaatgtag ccacgggcga
     2701 tagtgttgct gagtgtcaaa agaattatca agctttgctt actcgcgaag gaattgacat
     2761 gactggtggc ggactttctg gtaatcaaat caccggaact atcgcaaatt atatgactgc
     2821 tgtaattgat agtaatactc atgtttactt tacaattgcg ggcgatcctc gtatttttga
     2881 tgctgcactt ccaggtatga tgcaaattgt tagatataaa gttggcgacg caatttcatt
     2941 ctatgcaagt gaaagtggag agacttgaac tgttacgtcg ctaggttaaa tgaagcgcgt
     3001 taagataaat gacatatatt tggatgcgaa atcttttggt gaaaaagaga ttctagtttg
     3061 cggttgggtt aagtcaattc gcgatatgaa ggaatttgct tttgcaacta tcaatgatgg
     3121 aagttgcttt aaagatttgc aaattgtttt gagcaatgct gatctttcta attacgatga
     3181 ggcgactcat gctggaaatg gttcggcaat ttatgcaaag ggtacattga agttgacccc
     3241 tgatgctccg cagccatttg agcttgctgc agatgaattt gtaattgaag gtccttctgg
     3301 agaagattat ccgcttcaaa aaaagcgcgc gactcctgaa tttttgcgaa ccattcaaca
     3361 tttgcgtccg cgtacaaata tgttccgtgc aatttttaag gttcgaagtg ttgcggctta
     3421 tgcaattcat caattctttc aagatagagg ttttgtttac attcacacgc caataatcac
     3481 tgcttctgat tgcgaaggcg caggtgagat gtttaaagtt gatgctcctg gaaagaaaaa
     3541 tttcttcgga gccgatgccg cgctgacagt ttctggtcaa cttcaggctg aaaattttgc
     3601 aatggctttt ggtgatgttt atacttttgg tccaacattt cgtgccgaag attcaaacac
     3661 gccacgtcat gccgcagagt tttggatgat tgaacctgag attgccttcg ctgatttaaa
     3721 cgatgatatg caactcgccg aagatatgct caaaagcgta attgattatg ttatgaataa
     3781 gtgtccggat gaacttgaaa tgttaaacaa gtttgttgag aaaggtcttc ttgataaatt
     3841 gaagagcgtt gcaaaggcga agttcgtccg ttgttcttac accgaagcta ttgagattct
     3901 tgagaagtct ggagagaaat tcgattaccc aattaagtga ggcgttgatt tacaaactga
     3961 acatgagcgt tacttaactg agaagcattt taagtctcct gtttttgttt atgattatcc
     4021 agccgaaatt aaagcttttt atatgcgcat gaatgatgat ggtaaaactg ttgcggcttg
     4081 tgattgtttg gttccacaaa ttggcgagat cattggcggt tcgcaacgtg aagagcgtct
     4141 tgatgtttta gagaaacgca ttcgcgatct tggaatgaat ccagaagatt ataaatatta
     4201 tttagattta cgccgttatg gttcgtgtaa acatgcaggt tttggtcttg gcttcgaacg
     4261 ccttatcatg tatctaactg gcgcatcaaa cattcgcgac gtgcttccac atcctcgcac
     4321 tgtcggcaac gctcaattct aatttatata agaaatgtag tctgggttga tgtcggggtc
     4381 gctgcgtttt tcgcctgctg gataaccaat tgctaggttg ccaacacctt cgtagttacc
     4441 ctcaagccct gctttcttta atacatcttt gccttctggc atttcgaaga attccttagc
     4501 gcgatgaatt cagcatgagc caatgccaag ttcttcggcg gctaacatca tgttacccat
     4561 aactaggcta ccatcataaa catatgtgtg gacatcttta tttgcaacta cacaaataat
     4621 tgttggcgca ccataaaatg gatcaacagc accgcgtcca aaaatctcag catttttctt
     4681 tgaaattaaa tcacgaattt ctttattatt aacaacaata atttttgccg cttgcttatt
     4741 cattccgctt gcagctttgg ttccggcttc gcaaatcttc tgaataatgt cctctggaac
     4801 tggcttttcc ggatcgaagt ttttgcaact tcttcgacta ttaattgctt ctaaagtatt
     4861 catgataacc cctttctatt agtattttac atcaagcggt gcgtgtgctt caacatcgct
     4921 atccaaattt gaaaactttt tgtttgcgaa tcgttcatta gctacaagcc caatcattgc
     4981 ggcattatct ccgcatgccg aaagaggcga cattaccatt tcaacatttt cttccttaca
     5041 cattttttca taagcgctcc gaagcgaagg atttgctgca actccgcctc caaaaaggaa
     5101 tgactttgac tttgtttcct gtattgctct gcgggctttg gcgatttgga cttcaattat
     5161 ggcttgttcg aaacttgcgc acaaatcgtt tagattgaag tctttatcat ttttatgttc
     5221 gtcaacataa tttataactg cagttttcaa acctgaaagg gagaaattaa agttgccttc
     5281 ttttatcatt gctcgcggaa atttaattgc atttgggtct ccgtctttgg caagtttaga
     5341 aattacagga ccgccagggt atccaagtcc caaatatttt gcaagtttgt cgaaggcttc
     5401 gccggccgca tcgtcgattg tagtcccaag agttttatag ttgcctcaat ctttaacgtg
     5461 aaccagaagt gtgttgcctc cagaaattat cgatgctata gcaggaagtt taatatctgt
     5521 ttgcgcaagt ttatttgcat aaagatggcc ctctaaatga ttaacaataa tcaaaggttt
     5581 ttctaatgca aaagcgattc cttttgcaaa agccattcca actaccaaag caccaacaag
     5641 acccggggca taggtgacag caatcgcgtc taaatcagat caattgaatt ttgatttctc
     5701 aatgcattca tcggcaacgc cacagattgc ttcgatatgt tttctgcttg caatttctgg
     5761 aacaacaccg ccaaagcgtt tatgaaaatc aacttgcgaa gcaacaacat cgctgacaac
     5821 ttcaccatca caagtaattg cgcacgcagt ttcatcacac gaactttcaa ctgaaaaaat
     5881 tatcggatgt cgaacatctt ttacggcaga agaatttgaa tctgttttta aattcattcc
     5941 accaacgtct ttagtcaata ttggaagagg tcctccgaag acaagcgcat cttcaccatc
     6001 gctataatat ttttctcgag tagcaatttg cttcattcca actttttgat aaaacttttg
     6061 cgctactgaa ttagtctttc gaacttctaa aattatttct tgcgcgccaa ggtctctcat
     6121 gtcatttgaa acataagaaa tgatatgttt tgcaatacct tgcttttgat gtttattaga
     6181 aactccaatt ttaagaagtt ccattttatt gtctgcttgc attgcacctg cataagcaac
     6241 aatattttcg tcttgcttgc aaactcacca agatctgtta tttagcttaa gttcgtcttc
     6301 gaatttctta ttgtttcatg catcggtacc catcaattgt ttttctaatt ccacaatttg
     6361 cgggatgtcc tttgcatcaa gcggttcaaa gtttttattt tcgccttcta aattttgtac
     6421 accagtattt aaattctttt tggaaatatc cgcgagtttt tcccgctcac tttcctccgc
     6481 atcactcaac cttgtataca ctggcaaaat ttccccggca ttcttttgat tttgaccctt
     6541 gcatacatga attaaacttt gcgccgaagg aactcacaat tcttgattgc caatgtttaa
     6601 gccttcgaat aagtcaaaat atttatgaag ggcgtcacca acaattgtta aattatcaac
     6661 tttggttgat agctcaaatg cttcgtcagc actagcttga gcctttagaa ctttatcagg
     6721 agagagacgt ttgacacctt cttcgcttag ctcacacaaa agaggataga tttcatgacg
     6781 cattgcgtca gcaacaacga agacgtgtcc tcgaattcca gattctcaag cgtttcatgc
     6841 aaccgcttca agtgtgttaa agcctattaa cggaatttga agagcagttg caattccctt
     6901 agcagttgcc aaagcaattc ggacaccagt gaatgaacca ggtcctctac cagctgcgac
     6961 acatgcaata tcacttttag aaactttgga tttcttaaac agtttttcaa cttcaggcaa
     7021 aagcatcgtg tttgaagctc gatgagcttc gccacaaaaa gatgcgcaca gcgaaaaatc
     7081 atctaaatta gcaattgcaa ttgaaattga ttcatttgca gtgtcaaaag caagtatgta
     7141 tttattatca ctcattgttt tcttattata taatacgctt tgcaaggagt aatgatgatt
     7201 ttagctgttg acattggaaa tacacagaca tgcgttggtg cgttcgaaaa tgattcgaat
     7261 gacttaaaga gtttgtggag gttttcgacg cccgaaaaaa ttacggttga cgaagcaacg
     7321 tctgacttta tgcaaattat tgatcaaagt gaatttagtg ttgaagatgt gagcgctgtt
     7381 tgtatttctt gcgtagttcc aagtttgaca gttttctaca ctaaggcaat taatgctttt
     7441 attcaaaaac gaaatttgga tgtaaagttt gttgtttgta atgctaaaaa ttctgagaag
     7501 cttagcaaaa gtttgtacaa ttgtaaatat gcgcatccga atgaagttgg cggcgatatt
     7561 attgcaactt gtgttgccgc gcgagtttta tatgacatgc cttgcgcgat tatcgacttt
     7621 ggaaccgcga caaatattaa tgttataaat tcaaagggcg aatttgttgg tgccataatc
     7681 tcccctggtc tcaagacttc tttgaacgct ttggttgccg atgctggcgc cttgagtgat
     7741 atcgaaatta aggttcccag caaacttttg ggaaattcta cacctgaact aattcagagt
     7801 ggaagcataa ttggcgaagc cgcgcgcgcc gatggtttta ttgacaggat cgaagaggac
     7861 ctaggtgaaa aagttaatgt aatttgtacg ggcggttgat cgaagttgct tcacagcgaa
     7921 atgaagcatg atgtaatttg gaatccgagt ttgatcttac aaggtttgaa aatcttcgtc
     7981 gagaacttcg atgcctaaaa attctgaaaa taaaattcag cgaattaaga atgaacttaa
     8041 tcacgttcct tcgaagcctg gttgctattt gtgaaaagat aaagacggtc aagtaattta
     8101 tgtagggaag gcgaagaatc ttcgtgcacg tatgcgacag tacgtaaatt tctctgatga
     8161 acgcgaaaaa attcctatgc ttgtggaaga aattgatagt tttgattaca ttgttgttca
     8221 aaatgaaagt gaatcgttaa ttcttgagaa gaatttaatt aatcaatata agccatttta
     8281 caatgctgac tttaaggatg ataaatctta tccttttatt gcaattaccg aaggcgatca
     8341 atttccggct atcaaatata ctcgagaaaa acatgttcct accacgcgtt attttggtcc
     8401 ttatacagag agccgtgcgg cgcgtcaaac ggttgatatt gtgcgacgaa tcgtaccaat
     8461 ttgtattact tcttgtgtag catggaaaaa tattggcaag cgaaaagctt ctaaaccatg
     8521 cttcgattgt actgtgggtt taggtccggg agcttgttgt gacaaaatca ctccggaaga
     8581 atatatggaa aatgttaaag cggttgagcg gtttttaaat ggaaaccaga gagaatttat
     8641 agaaaattta gaaattgaaa tgaaaaatgc cgcgaaaaat ttagattttg agcgtgctga
     8701 cagaattaaa aaacgaatcg aaactgtcaa tcttttaacc gagaagcaac acatcgttag
     8761 ccaacaaaat ttaaatgcgg atgtgattgg tttttatcgt gaagaaacaa tcgccggcgt
     8821 tcatgtttta attgttcgtg aaggacgcat aattaatagt aacgaattta ttttgaacag
     8881 aggtcttgat gttgaaaatg aggatttagt ccacaacttt ttacttcgtt attatgacac
     8941 cacaacaagc attcctcaaa gaattattat gcgcgaaaat cctgaagata cagatgaaat
     9001 gtctgaatga cttacaggaa agcttgatag ttctcacggc gctaaggtaa agtttgaagt
     9061 tccaaaaatt ggcgaaaaga tggacatgct taaaatggcg gagctaaatg cgaagcattc
     9121 gcttatgcga tacaaagtta gaacagggta tgatgacaag cgaattaatg atgcactttt
     9181 gcaattggaa agcgctttgg ccatggaaaa aagtccaatg cgaattgaat gttatgatat
     9241 ttcaacaatt catggttctt acaccgttgc ttcgatggtg actttttctg gaggtaaacc
     9301 agataaaaat tcttacagac gttttaagat taaaactcca cttcgcgaag ctaatgattt
     9361 tctttcaatg caagaagttc ttcgtcgtcg ttttgatgtg aagcgtcgtc aagataagcg
     9421 ttttgctcaa aaacctgacc taattttgtt ggacggagga aagcctcaat taactgccgc
     9481 taaaaaaatc tttgaagaaa ttggtttaga cattaaaaaa gaaggaattg ctcttgccgg
     9541 attagcaaag tcagacgaag aattaattgt tccgtttggc aatgaatatg aaactgttgt
     9601 tttgcctgac ggttcggctt cgttgtattt agtaaagcaa gttcgtgatg aagcgcatag
     9661 gtttgcaatt acatttcaca ggcaacttcg agcgaaggga atgaccgcct caattcttga
     9721 cgaagttcct ggccttgggc ccgttcgcaa gaaagcactt ttaaaagcgt tcggaagttt
     9781 taaaaagctt cgcgaagcat cacttgaaga aattaaatct aaaaaagtaa ttccagtaga
     9841 tattgctgat gaattatttt tggttttaca ttcagataaa tagaagatag atactcgcgg
     9901 gtccgtggta taatgaagcc atggataaaa ctaacgcaaa agaaaatctt gacgaagcaa
     9961 ttaaggaggc tcctacagca gcagatgctg agaaaaagga agctgaaatt tctaaagctg
    10021 taaatgctga aacaattgcc gaagaaaact tgccagagtt gatgattatt actggtatta
    10081 gtggtgctgg caggactgag gccatgcacg tttttgaaga cttaggttat ttttgcattg
    10141 acaatttgcc agcatttttg ctttcggaat tggttgagaa ggagcgcgaa aacaatcctg
    10201 aaaagatgaa gattgctgtt gtttgtgatg caaggaacag acagtacttc gttagtttgc
    10261 ttaaagagat cgaaaaagtt ttggacatgg gtgtggatta caagattttg tttttggagg
    10321 catcggatgc aaaacttatt tctcgttata aaagttcgag gcgccgtcat ccaatgtgtg
    10381 agcctggcga gactattgtt caaggtatcg aaaaggagcg cgacctgctc tttggcttaa
    10441 aggaaattgc gcaatatgta attgatacgt ctgacatgtt gccgcaggat tttcgcgcgg
    10501 agcttcgtaa aatatttaat gccgatgaaa aaattgaagg cttgtccatc actgtttatt
    10561 cctttggctt taaaaatgga tctccagttg atgcggattt ggtaattgat gttcgttttt
    10621 tgccgaaccc atattgagat cctgaaatgc gacctctttc tggtttggat actcttgtgc
    10681 atgattttgt tatggcacga agtgaaacgc aagacttttt ggaaaagtga cacggccttt
    10741 tagattcgct gattcctggt tatgtgaagg aaggtaagca gcaacttgct atagcaattg
    10801 gttgcacggg tggtcagcac agaagtgtaa caatcgccga agccactggt ggttacctca
    10861 aaagtcaagg ctacagggta agcattgcac atcgcgatct tgctatggcg gttgctcaag
    10921 ctgttaatcc aagcagcact ggttcctgaa ggccaatagg cagatagagt tcccatgtca
    10981 tttactactg atgtcaaaga cgaattatct cgtgtcgagc cagaatgcaa aacatgttcg
    11041 aaggcaatgc tttcggctct tgtaaaaagt cacggaactt taaatatttt gggtaaggga
    11101 aatttcaccg ttcgatttgc aacagacaac gcgtggattg cgcgattcgc tttggaaaat
    11161 tttaaggttt tatataactt agaaacgtct cttacttaca gacgaagtgt tttgcacaac
    11221 actcgaaatt atttgattga gcttaactct gggccaaatt tgcaaaaagc gctgattgac
    11281 atgggtattt tgcaagctcc gaagaaggat aaaaccaagg gtgtggcttc gttttctcat
    11341 ggagtaaaaa aagattttaa ggacaaagat tgttgtgcgg cttcgtatat gcgaggagca
    11401 ttcttgggta gcggctatgt ttctgagcct tcgggggatt ttcactttga aatttcagtg
    11461 gacagaaaat ctcaagcaat tgatatgcaa aaaattttgg cgaagaagga tataaagtcc
    11521 ggcgtgtgtc cgcgtcgaaa ttcttatttg ctctatatta aaagtggtca aggaattgca
    11581 gattttttgg ctttcactgg tgctcacaaa tgtgcgctta aacttgaaag cacgcgcgtt
    11641 tcaaagcaga ttgcaaatca aatcaaccgc caaacaaatg ctgaaattgc aaattcgaag
    11701 cgaacggtcg aagcggcgta taaccaaatt gtaatgataa agcgtgttgc aaaacattat
    11761 ggtttggaaa acttgccgcc tgcgcttcgg gagtttatgg cacttcgtgt taaatatcat
    11821 gatgtttcac tttctgaact tggaactttg gctgatccgc caatttcaaa atctgctatc
    11881 aacggacgag ttcgtcgcct tgaacacctc gctaatacta ttaaataatg tgctaaaata
    11941 aatccgctat aaaatctaat aaatcaagga gggaaaatga ctgcaaagta tgatgctgtt
    12001 gtttgtggtt cgggaaacag tggtttaact gcttcgttga ccttggcagc agcaggtaaa
    12061 aaagtgctat tgattgaaca gcacaatttg ccggggggct ctgcttcgag ttttaggcgt
    12121 ggtcgcttcg aacttgagcc aagtttgcat gagcttggtg aggtcggtcc gaagaacaac
    12181 cctggtgctg ttcgcaaaat gtttgatcaa cttggcgtaa aagttgattg gattgaaatt
    12241 ccagatttgt tccgcgttgt ttcaacttgg acagatga
//
LOCUS       DBNCDC010000023        11673 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_2128_length_11673_cov_7.580995, whole genome shotgun sequence.
ACCESSION   DBNCDC010000023 DBNCDC010000000
VERSION     DBNCDC010000023.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 11673)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11673)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11673
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2128_length_11673_cov_7.580995"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            53..418
                     /locus_tag="AC04VQ_02840"
     CDS             53..418
                     /locus_tag="AC04VQ_02840"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIO9553139.1"
                     /translation="MKLQAIAQAGLYYTKNEFERERYREIRKIACEILAEHSNIEIDE
                     VTEYFTPETGYQTPKLDSRSVCFKDGKLLMVQESFGQWTIPGGWIDVDKKLSENLVKE
                     AKEEAGAEVEPNFVIALSD"
     gene            479..643
                     /locus_tag="AC04VQ_02845"
     CDS             479..643
                     /locus_tag="AC04VQ_02845"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIO9553140.1"
                     /translation="MCDLKSMNFKSNSETLQADFFDRDNLPDIAVGKNSKDQIELCFK
                     AYDAVSSGKH"
     gene            674..1102
                     /locus_tag="AC04VQ_02850"
                     /pseudo
     CDS             674..1102
                     /locus_tag="AC04VQ_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014598.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
     gene            1115..1510
                     /locus_tag="AC04VQ_02855"
     CDS             1115..1510
                     /locus_tag="AC04VQ_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIO9553141.1"
                     /translation="MLAILTPDVLNETFWIVVIIIYYFIATLVPIDKVIGHLYPLFGA
                     VLILMTIGILGGSLIEYNFAIPEIEFTNMHPEGLPIFPFMFITVACGAISGFHATQSP
                     MISKCITSEHQGRFVFYGAMISEGIIALA"
     gene            1598..2128
                     /locus_tag="AC04VQ_02860"
     CDS             1598..2128
                     /locus_tag="AC04VQ_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIO9553142.1"
                     /translation="MSTGMLGQVGGVLAIIGVVACPITSGDTAFRSARLIIAEIFNLP
                     QKEINKRLYIAFPLFIAGFAFTQLNFDVLWRYFSWSNQTLAMLALAVFTAYLLKKHKN
                     KFVSLITVIPCAFMGAVTLSYILMAEEGFRLSATIAYPVGILFALAILVFYVMKLVRA
                     IRISKGIYKPKKTEVI"
     gene            2156..2647
                     /locus_tag="AC04VQ_02865"
     CDS             2156..2647
                     /locus_tag="AC04VQ_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013205.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553143.1"
                     /translation="MSGQFIDKVRIFVKGGNGGAGCMSFRRESHVPKGGPDGGDGGFG
                     GSVILQADGGKSSLIEYRYKHHFKAERGTHGKGSKRNGQNGEDLILKVPVGTLVREVL
                     TVGEAEDKSAKSGAVIADLTQNDQRVVVSQGGTGGRGNTHFVTPTRRAPEFAELGEPV
                     EGR"
     gene            2669..2944
                     /locus_tag="AC04VQ_02870"
     CDS             2669..2944
                     /locus_tag="AC04VQ_02870"
                     /inference="COORDINATES: protein motif:HMM:NF014036.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HIO9553144.1"
                     /translation="MADAALVGMPSAGKSSLIARLSAARPKIADYPFTTITPNLGVAK
                     AGDLSYVLADIPGLIEGASKGKGLGHEFLRHIERSALIVHIVDMTGS"
     gene            3053..3571
                     /gene="cgtA"
                     /locus_tag="AC04VQ_02875"
     CDS             3053..3571
                     /gene="cgtA"
                     /locus_tag="AC04VQ_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HIO9553145.1"
                     /translation="MPDTQDNVQRLKEQVAKDVKDAEGSEKEGLINPKVFEISAVTGM
                     GVRELNLAIANKVAEIKAQRQENEENQETYEKVWTLDKRDEDEFEISKVSAGVFEVKG
                     KRPVRAVVQTDLDNEEAVVFLQHRLKRMGVERALAEAGAIDGDEIQIAGRSFEFENTE
                     NSTVKDEYGDLI"
     gene            3572..4021
                     /locus_tag="AC04VQ_02880"
     CDS             3572..4021
                     /locus_tag="AC04VQ_02880"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013122.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIO9553146.1"
                     /translation="MATTKETLNEFLREGTPAAVVERYIALEQIAEDSNFGSLDNNIV
                     VVDVEATGLSFVKDELIQIAAAKINNGKIEDWYVTFVNPGMEISEDIEHLTNISNDDV
                     ADAPGPEDALAGLVDFAGDAVMLAHNADFDKTFLTKHAAGYPLLENI"
     gene            4037..5221
                     /locus_tag="AC04VQ_02885"
     CDS             4037..5221
                     /locus_tag="AC04VQ_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553147.1"
                     /translation="MVRIAFPRLTSHRLIDLSKAFGVIESTHRADDDVATTCVLYRLV
                     LAAITQMPLNLIQVIAGLAPIEMWSTGYIFKEMLKILPTLGLDDAEVEDDYVVPVDVY
                     DKDLFGNEKTKVNTFGMLRTMRNERFQNLPKQKKYDIALDDEAPMGADENTPIKAADA
                     LQFPNEESIRSEFSKSGMLGKIYPDYEQRDEQVTMSAEVLSAFRDSANLCIEAGTGVG
                     KSMAYLIPAIKIAKENEITIGVATKTNSLLDQLIYHELPALSDQIDGLVYSSLKGAKH
                     YICLRKAAYLAGAEAKIVKFKGEEFCNAASIAGLISYIEQTAYDDCDGLKINGRALPN
                     AAYTCGSHECLRNKCPFYHNGCFVHGARRMAKNSDIVVTNHSMLFCDIKANNGLLPPI
                     KY"
     gene            5297..5536
                     /locus_tag="AC04VQ_02890"
     CDS             5297..5536
                     /locus_tag="AC04VQ_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553148.1"
                     /translation="MDFGKSLNSESAKYNVYERAARNLNTEEFDDKGTVFGLLNKAKS
                     AGKALAHESDIFANSICDLLDFAQKSNGGYEYSTV"
     gene            5618..6214
                     /locus_tag="AC04VQ_02895"
     CDS             5618..6214
                     /locus_tag="AC04VQ_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553149.1"
                     /translation="MITETNKLLAYLEDFKTATVISREISAFVFDVKEVLNACDVIFI
                     NNSDKYVYSAFMNTKKTVKQDKLCAELYSVSEVLNEELYGQCKSIVFTSATLATGDSF
                     KSFKDSIGLPESTRECQLSSSFDYDKNMTIFVANDMPGNPNLPEFSDKLTQFLTDIHI
                     AGDGSILSLFTNRRQMERAFETVSEDIKPRGLRLLMQK"
     gene            6218..6625
                     /locus_tag="AC04VQ_02900"
     CDS             6218..6625
                     /locus_tag="AC04VQ_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HIO9553150.1"
                     /translation="MSVKGVKDEFLSNEKLSLFALKSFWEGFDAPGSTLKGVIICKLP
                     FTRIDDPLNLERRAREDNSWFKYTLPSSVIETKQAVGRLIRKADDKGYVVFADSRLVQ
                     KSYGELFMRSMPSQNIKIMSCNEIVDEVSKLEF"
     gene            6626..7780
                     /locus_tag="AC04VQ_02905"
     CDS             6626..7780
                     /locus_tag="AC04VQ_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553151.1"
                     /translation="MARKKKKNLENKENVDHSQHGASHLTSDKSASFQEVSFDVLENA
                     VERRNSLFGYKSRDFRNRAIISSDEMLSRKSGNAKSVGKLAVIVLFVILGLGIIAGLM
                     LYLNNNGYFTKIKHSTPLKEAIAEIGDYDYEIMRLNAFLQTSINESKISQANFDKKDY
                     EDIKKNLDDLSNKITDIAKGIDPQSEDYVYAGYAKQTIEARDMMIDSGLKIYDNAVES
                     VDVINKTEQMWNNVLKADASLKESDNLVQSNSSDNYALAKQDSEQAVTNLTEASTQIS
                     TLLKLTKAIDFSIYSKYISARLESAIISVSACQALLDQNLDQISSLNLTYIQKSNIAS
                     DIALSMSTTPVQVLRADYDIRTRPLIEEFKAARKNAADNDFALRNFLAAD"
     gene            7871..9013
                     /locus_tag="AC04VQ_02910"
     CDS             7871..9013
                     /locus_tag="AC04VQ_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553152.1"
                     /translation="MSSMLDDVSHLSREIGARPAGTEEERLAAMFIAENIQKRSGLPF
                     DIEDFEGAKGSSRVTNVLSIVSAILLFLAVLLPATTIPALVLTLGIAIICILEYFNRP
                     ILSNFFRYGISQNVVAKYVPAYSSDSAQTQRKRKVVFVSHYDSGRSMPDNSDAILNLS
                     YWSKILALASDCIIVLFLLIKLFAFPETSGALTLFCVIFSFVLAIICVLPSLVDLYKG
                     RLLYNEGANCNASGNAALIEIAKQLGTGAYSTDEDFESRKPRPVVHGEKAAKEAQVIP
                     EGSNVTYETGTSKDQKVDVLEEKEQSLEDAKAAIAAFTAPRAPRAKYDDEGNVVDEGL
                     KTEEKPDISNVTIDDATAAREEIKADTTASMPAYKTESSKSGDVPD"
     gene            9104..10732
                     /locus_tag="AC04VQ_02915"
     CDS             9104..10732
                     /locus_tag="AC04VQ_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553153.1"
                     /translation="MDVMAARQEEEEAKKKAEEEKKRAELRAQIEAANKAAEEERQKK
                     FGDKKPFYRSEDSPEDTQGMDAIEEEKPQESQEELTIDESFKQRVAKDAKPAKEENKE
                     EAKEEDKSEDKLKPEDSGQIRVDQMQQYAPLDDQEFISSNEMPQNEALTELPEVYTDE
                     KNIEENPEYAKALKEDDAKNEQKEEAKVEEVEKGKFGTGSFAAVSESEGVAGATGTFA
                     PVQEELIVDASKSGEIANKDDMVVADADDSVYSQGEFTDTGAFAGKGYVDMPDEKKKG
                     ILGRFGKKDKGAGKHASQAEVVEPVNNDADNVIDDAEWEGGAFSNIAGKIKLPKKNKN
                     EEEPAFEENVVAEDMPVDNDVEYSEDYETDPDAPITSSESRINSMVDPVPDFDQQIQD
                     FHNASINMEVWMVALGSEIDENAGIKAFLMEHAQELRGAIIIDIEALGAGELSLINSE
                     GVIRKSQTSSRMKRYVRSAANKLGVVIQSVDVPWGMSSAAYASKCGYKTLRLAGMDGK
                     KPALYMSKNDVVENVDENKLKENVKYLLQLIQTI"
     gene            complement(10888..>11673)
                     /gene="holA"
                     /locus_tag="AC04VQ_02920"
     CDS             complement(10888..>11673)
                     /gene="holA"
                     /locus_tag="AC04VQ_02920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIO9553154.1"
                     /translation="LLPVYAIVGDDKLKSKVVLERLSKRMEEFGDMSFNSSTFDGEKA
                     IGTEITSACMQIPFASEKRFVVVKNADKLKKDDAERIVEYLENPNPTTILALVCTKLA
                     KNSRLFKACTKISNTSIIDCSSVKTYLVADSLNNIARSHGGGIDKNAAQRLVELVGPD
                     TLKLDAEIQKLLLSNGNQNITLQQVNDEVQKSAESKPWDFTNAFAARDLNKCLQLFQE
                     MSSGSEYMLLPQTCKIVKELICVQDLGQVATQNAISQALGYEA"
BASE COUNT         3603 a         1854 c         2578 g         3638 t
ORIGIN      
        1 gcagagcatg tcgaataaga ttcaaacaaa agaaaaatga aacgaatgag caattaaact
       61 tcaagcgatt gctcaagctg gtttatatta cacaaaaaat gaatttgaac gcgaacgcta
      121 cagagagatt cgcaagattg cttgtgagat tttagccgag cattctaata ttgaaatcga
      181 cgaagttaca gaatatttca caccagagac tggttatcaa acaccaaaac ttgacagcag
      241 gtctgtatgt tttaaagatg gcaaactttt aatggtgcaa gaaagttttg gacagtggac
      301 tattcctggt ggatggattg atgtcgataa aaagctttct gagaatttgg ttaaggaagc
      361 aaaagaagag gcgggtgctg aagtggagcc caacttcgtt attgcgctga gcgattgaaa
      421 atctcacatt ccagttaaat tcgagaattt accatttcaa atttgcaagg tttttgtaat
      481 gtgtgatttg aaaagcatga attttaagtc aaattcagaa actctgcaag ccgatttttt
      541 tgacagagac aacttgccag atattgccgt aggaaaaaat agtaaagatc aaattgaact
      601 ttgctttaaa gcatatgacg cagtttcttc tggaaaacat tgagatacag tattcgatta
      661 atgtgaggtt taaatgtttt ctcttttagc tagtattgct gttttaatta ttggttattt
      721 tacttacggc aagtttgtcg agaaagtttt tgttcccgat gatagaaaaa ctccggcatt
      781 tactgaaaac gatggcgttg acttcgttcc tttaaaactt tgaaagtctt atcttattca
      841 gcttttaaat attgcaggta ctggtccaat ttttggagcg ctaatgggtg cggtttttgg
      901 gccagttgta tttttatgaa ttgttttcgg atgtattttg ggcggtgctg ttcatgatta
      961 catgacagga atgatttctg aacgaaacaa aggtgcttcg attgccgagc tttctggaat
     1021 ttatttaggc aaatctgtca agtggatcat gcgtctattt agcatcattc ttcttgtatt
     1081 ggtaggtgct gtttttgtta ccagcccagc aaatttgctt gcaattttaa ctcccgatgt
     1141 attaaacgag actttttgga ttgttgtaat aattatttat tattttattg ctactcttgt
     1201 cccaattgat aaagttattg ggcatttgta tccattattt ggagccgttt tgattttgat
     1261 gactattgga attttgggcg gaagtttgat tgaatataat tttgcaattc cagaaataga
     1321 gtttacaaat atgcatcctg aaggcttgcc aatctttcct tttatgttta ttactgttgc
     1381 ttgtggtgca atttcaggtt ttcatgcaac tcaaagccca atgatttcaa aatgtattac
     1441 tagcgaacat caaggaaggt ttgtatttta tggagctatg atttctgagg gaattattgc
     1501 tcttgcatga gcagcggcag gtgtttgttt ttacgaaggc acaggcgggc tgttaaatgc
     1561 attaaatact gcgggtcaga gtgcaactgt atataatatt tctactggaa tgcttggaca
     1621 ggtcggtgga gttcttgcaa ttattggtgt tgttgcgtgc cctattactt cgggggatac
     1681 agcttttcga agtgcaagac ttattattgc cgagattttt aatcttccac aaaaagaaat
     1741 taataaaaga ctctatattg catttcctct atttattgca ggatttgcat ttacacaatt
     1801 aaactttgat gttttatggc gttatttttc ttggagcaat caaaccttgg caatgcttgc
     1861 gctagccgta tttactgctt atttattgaa aaagcataaa aataaattcg tgtctttaat
     1921 cacagttatt ccttgcgcct tcatgggtgc cgttacttta tcttatattt taatggccga
     1981 agaaggtttc aggttgagcg ctactattgc atatcctgta ggaattttat ttgctttggc
     2041 aatacttgtc ttctatgtta tgaaattggt tcgtgctatt cgaatttcca aaggcatcta
     2101 caagcctaag aaaacagaag taatttagac atctacctgt tataataggt gggctatgtc
     2161 aggacaattt atagacaaag ttagaatttt tgtaaaaggc ggcaacggtg gcgctggctg
     2221 catgagtttt cgtcgtgaat ctcacgttcc taagggtggt cccgatggcg gcgatggagg
     2281 ttttggcgga agtgtaattt tgcaagcgga tggcggcaag tcttcgttga tcgaatatag
     2341 atataagcat cattttaagg ctgagcgcgg gactcatgga aagggctcta aacgaaatgg
     2401 ccaaaatggt gaggatttga ttttgaaagt tcctgttggc acattagtac gtgaagtttt
     2461 aactgttggc gaagccgaag acaagtctgc caaaagtggc gctgttattg ctgatttaac
     2521 tcaaaatgat cagcgtgttg ttgtatcaca aggaggaact ggaggcagag gcaacactca
     2581 cttcgttact ccaacaaggc gagctcctga atttgcagaa ttaggggagc cggttgaagg
     2641 cagatgaatt gaacttgaaa tgaagttgat ggcagatgca gctttggttg gaatgccttc
     2701 ggcgggaaaa agttctctca ttgcaagact tagcgcggct cgaccaaaga ttgcggacta
     2761 tccatttaca acaattactc caaatttagg agttgcaaaa gctggagatt tgagctatgt
     2821 tttagctgat attcctggac ttattgaagg cgcttctaaa ggcaagggct tgggacatga
     2881 atttttgcga cacattgagc gaagcgcttt gattgttcac atagtggaca tgactggaag
     2941 ttgagaaggt cgcgatcctg ttagtgatta taagacaatc aacaaagaac tttcgcttta
     3001 tgcaaaagat ttaagtttaa gacctcaaat tgttgttgcg aacaaatgcg aaatgccaga
     3061 cactcaagat aatgttcaac gattaaaaga acaggttgca aaagatgtta aggatgcaga
     3121 gggcagtgaa aaagaaggct taattaatcc aaaagttttt gagattagtg cggtcaccgg
     3181 aatgggagtt cgcgaactta accttgctat tgcaaataag gttgctgaaa ttaaagcgca
     3241 gcgacaagaa aatgaagaga atcaagagac ttatgaaaag gtttggactt tggataagcg
     3301 cgatgaggat gaatttgaga tttctaaagt ttctgctggg gtctttgaag ttaagggtaa
     3361 aaggccagtt agagctgttg ttcaaaccga tcttgataac gaagaagccg tggtgttttt
     3421 gcaacatcgc ttaaagcgta tgggtgttga gcgtgcattg gccgaagccg gagcaattga
     3481 tggtgatgaa attcaaattg caggacgaag ttttgaattt gaaaatacag aaaactctac
     3541 agtgaaagat gaatacggag atttaattta gatggcaaca acaaaagaaa cactaaatga
     3601 atttttgcga gagggaacgc ctgcagctgt tgtggagcgc tacattgctc ttgagcaaat
     3661 tgccgaagat tcaaattttg gatccttgga caataatatt gttgttgtgg atgttgaagc
     3721 aaccggtctt tctttcgtaa aagatgaatt aattcaaatt gccgctgcaa aaattaacaa
     3781 tggaaaaatt gaagactggt acgtgacctt cgttaatcca ggaatggaaa tttctgaaga
     3841 tattgaacac ttaacaaata tttcaaatga tgatgttgct gatgctccag gccccgaaga
     3901 tgctcttgcg ggtttggttg attttgcagg ggacgctgtt atgcttgctc acaatgcaga
     3961 ttttgacaag acatttttaa cgaagcatgc agcaggctat ccattgcttg aaaatatttg
     4021 agttgacaca cttgatttgg ttcgaattgc atttccgcgt ttgacttcgc atcgtcttat
     4081 tgatttgtca aaagcttttg gtgtaattga aagtacgcac agggcagacg atgatgttgc
     4141 aactacttgt gtgctatacc gtcttgtttt ggcagccatt actcaaatgc cgcttaactt
     4201 aattcaggtc attgctggac ttgcgcctat tgagatgtgg tctactggat acatttttaa
     4261 agagatgcta aagattttgc caactcttgg gcttgatgat gcagaagtgg aagacgatta
     4321 tgttgttccg gtagatgttt acgataaaga tttgtttggc aatgaaaaaa ccaaggttaa
     4381 tacttttggg atgcttcgca ctatgagaaa tgaacgtttc caaaatttgc ctaagcaaaa
     4441 aaagtatgat attgcgcttg atgacgaagc gccaatgggt gccgatgaaa atactcccat
     4501 taaggcagca gatgctttgc aatttccaaa tgaagaaagt attagatctg aattttctaa
     4561 gagcggaatg cttggaaaaa tttatcctga ttatgaacag cgtgacgagc aagtaacaat
     4621 gtctgcagaa gttttgtcag catttagaga ttcggcaaac ttgtgtattg aagcggggac
     4681 aggtgttgga aaaagtatgg cttatttaat tccggccatt aaaattgcga aggaaaatga
     4741 aataacaatt ggtgttgcta caaaaactaa ttcgcttctt gaccaattaa tctatcatga
     4801 acttccagcg ctatctgatc aaattgatgg gcttgtttat tcttcgctaa aaggtgccaa
     4861 gcactatata tgtcttcgta aggctgctta tttagctggc gcggaagcaa aaattgttaa
     4921 gtttaagggc gaggaatttt gtaacgcggc aagcattgct gggttaattt cttatattga
     4981 acaaactgcc tatgacgatt gcgatggctt gaagattaac ggtcgagctc ttccaaatgc
     5041 ggcatatact tgtggcagtc acgaatgttt gcgcaataaa tgtccttttt atcacaatgg
     5101 ttgttttgtc catggcgcgc gtcgtatggc aaaaaattcg gatattgttg ttacaaatca
     5161 tagcatgctt ttttgcgata taaaagcaaa caacggactt cttcctccaa ttaaatattg
     5221 aattgttgat gaggctcatg gcaccgaagc cgaggctcgc cgtgcgtttt ctgcaaatat
     5281 tttagcaagc actcttttgg attttggcaa atctcttaat agcgaaagtg caaaatataa
     5341 tgtttatgag cgcgcagcaa gaaacttaaa cacagaagag tttgatgata agggaaccgt
     5401 ttttggtctt cttaataagg cgaaaagtgc cgggaaggcc ttggcgcacg aatctgatat
     5461 cttcgccaat agtatttgcg atttattgga ttttgcccaa aagagtaatg gtggatatga
     5521 atattctact gtttgaatta ataatgaaat tcgtgaaagt gaacaattta ataatttata
     5581 taaggttgct gataagtgat ataaggctgc tgagaatttg ataactgaaa cgaataaact
     5641 tttagcatat ttggaagatt tcaaaactgc gactgtaatt tcgcgagaga tttctgcttt
     5701 tgtgtttgat gttaaagaag tgcttaatgc ttgcgatgta atttttatca acaatagtga
     5761 taaatacgtt tattcagctt ttatgaatac taaaaaaacc gtaaagcaag ataagttatg
     5821 cgccgaactt tattcggttt ctgaagtttt gaatgaagaa ctttacggac aatgtaaaag
     5881 catcgtgttt acttcggcga ctttggctac gggggatagt tttaaatctt ttaaggattc
     5941 tattggtttg ccggaaagca ctcgggaatg tcaactttca agtagttttg attatgacaa
     6001 gaatatgaca atttttgttg caaatgatat gccgggcaat ccaaatcttc cagagtttag
     6061 cgacaagctt acacaatttt taactgacat tcatattgca ggagatggat ccattctttc
     6121 gttatttact aacagacgtc aaatggaacg tgcttttgaa acggtcagtg aagacataaa
     6181 gccgcgtggt cttcgtcttt taatgcaaaa atgagctttg tctgttaaag gtgttaagga
     6241 tgaattttta agcaatgaaa agctttctct gtttgcgttg aagagttttt gggaaggttt
     6301 tgatgcgcca ggttcaacat tgaaaggtgt aattatttgt aagcttccat ttactaggat
     6361 tgatgatcct ctaaatttag agaggcgcgc tcgcgaagat aattcgtggt ttaaatacac
     6421 actgccatct tctgttatag aaacgaagca agctgttgga cgtttaattc gtaaggcaga
     6481 tgataagggc tacgttgttt ttgcggatag tcgcttggtt caaaaatcat atggtgaatt
     6541 atttatgcgg agtatgcctt cgcaaaacat taaaataatg tcgtgcaatg aaattgttga
     6601 tgaagtttcg aaattggaat tttagatggc aagaaaaaag aagaaaaatt tagagaataa
     6661 agagaacgtt gatcactcac agcatggagc ttcgcactta actagtgata agtctgcaag
     6721 ttttcaagaa gtttcttttg acgttttaga gaatgctgtc gagagaagaa attcgctttt
     6781 tggatataaa agtcgtgact ttagaaaccg cgcaattatt tctagcgatg aaatgctttc
     6841 gcgaaaaagt ggcaatgcaa aaagtgttgg aaagcttgct gttattgttc tatttgtaat
     6901 tttaggactt ggaattattg caggtctaat gctttatttg aataacaatg gttactttac
     6961 aaaaattaaa catagcactc cacttaagga agcaattgct gaaattggtg attatgatta
     7021 tgagattatg cgactcaatg catttttgca aacttcgata aatgaaagta agatttctca
     7081 agctaatttt gacaaaaaag attatgaaga tataaaaaag aaccttgatg atttgagcaa
     7141 caagattacc gatattgcaa aaggcattga tcctcaaagt gaagattatg tttatgcggg
     7201 atatgctaag caaacaattg aagcacgaga catgatgatt gattctggac ttaaaattta
     7261 tgacaatgct gttgaaagtg ttgatgtaat taataagaca gaacaaatgt ggaacaatgt
     7321 tttaaaggcc gatgcatctt tgaaggaatc tgataatttg gttcagtcga attcttcaga
     7381 caattatgct ttggcgaagc aagatagtga gcaggctgtt acgaatctaa ccgaagcgtc
     7441 aactcaaatt tcaactctct taaagctaac aaaggcaatc gacttttcta tttatagtaa
     7501 atatatttcg gcaagattag aatcggctat tatttctgtt tctgcatgtc aagccttgct
     7561 agatcaaaat cttgatcaaa ttagttcgct aaatttaact tacattcaaa aatctaatat
     7621 cgcatcagac attgcgctta gtatgtcaac aactcctgtg caggtgcttc gtgccgatta
     7681 tgacatcaga acaagacctt tgattgagga atttaaggca gcaagaaaga atgctgcgga
     7741 taacgatttt gctcttcgta atttcttagc tgctgactaa ttaattagtt ttccttgcaa
     7801 taaatcctgt ataatcccta ttagttatgt gcaacataac catgctattt aaaaagttga
     7861 aaggttaatt atgagttcta tgttagatga tgtaagtcat ttatctcgtg aaattggtgc
     7921 gcgtccagct ggcacagaag aggaaagact tgctgcaatg ttcattgcag aaaacattca
     7981 aaaacgaagt ggacttccat ttgatattga agattttgaa ggggcaaaag gttcgagtag
     8041 ggttacaaat gttttgtcta ttgtctcggc aattttacta tttttggcag ttttgcttcc
     8101 agctactact attccagctc ttgttttaac tttaggtatt gcgataattt gcattttgga
     8161 atattttaat agaccgattc tttcgaattt tttcagatat ggaattagtc aaaatgtagt
     8221 tgcaaaatat gttccagctt attcttctga tagtgcacaa actcagcgca aacgtaaagt
     8281 tgtttttgta tctcactatg acagcggaag atcaatgcca gacaattcag atgcaatttt
     8341 aaatttatct tattggtcaa aaattttggc tcttgcttcg gattgtatta ttgttttgtt
     8401 cttgttaatt aaacttttcg ctttcccaga gacaagcgga gcattaactt tattctgcgt
     8461 aatattttca tttgttcttg ctattatttg tgtgcttcca agcttagttg atttgtacaa
     8521 gggtcgtctt ttatataacg aaggagcaaa ttgcaatgct tctggaaacg ctgctttaat
     8581 tgaaattgct aagcaacttg gaactggtgc ttattcaacg gacgaggatt ttgaatctcg
     8641 taaaccaaga cctgttgtgc acggcgaaaa agccgctaaa gaagctcaag ttattcctga
     8701 aggttctaat gtaacatacg aaactgggac ttcaaaagat caaaaggtag atgttttaga
     8761 agagaaagaa caaagtcttg aagatgctaa ggctgcaatt gctgctttta ctgcgccaag
     8821 agcacctcgt gctaaatatg acgatgaagg aaatgtggtt gacgaaggct tgaagaccga
     8881 agaaaagcca gatatttcta atgtaacaat tgacgatgct actgctgcaa gagaagagat
     8941 aaaagctgat accactgctt cgatgccagc ttataaaact gaaagttcaa agagcggaga
     9001 tgttcctgat tgatttaaat ctgcgcaaag caaagctaag aaaactgaga aagccgaaga
     9061 cgaagaaggg gctgtcggtc aacgttcaag atttgcacac actatggatg ttatggctgc
     9121 tcgtcaagaa gaagaagagg ctaagaaaaa agccgaggaa gaaaagaagc gtgctgaact
     9181 tcgtgctcaa attgaagctg caaataaagc tgctgaagaa gagcgtcaaa agaaattcgg
     9241 agataagaaa ccattttata gatctgagga ttcacctgag gatactcaag gcatggatgc
     9301 tattgaggaa gaaaagcctc aagagtctca agaagagttg accatagatg aaagctttaa
     9361 acaacgtgtt gctaaggatg caaaacccgc gaaagaagaa aataaggaag aggccaaaga
     9421 ggaagataag agcgaggata aattaaagcc tgaagattct ggtcaaattc gtgttgatca
     9481 aatgcaacag tatgctcctt tggatgatca agaatttatt agctcaaatg agatgcctca
     9541 aaacgaagct ttgactgagc ttcctgaagt ttataccgat gagaaaaata tcgaagagaa
     9601 ccctgaatat gcaaaagcgc taaaagaaga tgatgccaag aatgaacaaa aggaagaagc
     9661 taaggttgaa gaagttgaaa aaggtaaatt tggtactggt tcatttgctg ctgtttcgga
     9721 aagcgaaggt gtagctggtg ctactggaac ttttgctccg gttcaagaag aattgattgt
     9781 ggatgcaagt aagtctggcg aaattgctaa caaggacgat atggttgttg cggacgctga
     9841 tgattctgtt tacagccaag gtgaatttac tgacactggt gcttttgctg gaaaaggtta
     9901 tgttgatatg cctgatgaaa agaaaaaagg cattcttgga aggttcggaa agaaggataa
     9961 gggagcaggt aagcatgcaa gtcaggctga agttgttgag ccagtaaaca atgatgccga
    10021 taatgtaata gacgatgccg aatgggaagg tggtgctttt tcaaatattg ctggaaagat
    10081 taaacttcct aagaaaaaca aaaacgaaga agaacctgct tttgaagaaa atgttgtagc
    10141 cgaagatatg ccagttgaca atgatgttga atatagcgag gactacgaaa cggatcctga
    10201 tgctccaatt acaagtagcg aaagcaggat taattctatg gttgatcctg ttcctgactt
    10261 tgatcaacag attcaagatt tccataatgc ttcaattaat atggaagttt ggatggttgc
    10321 tttgggttct gaaattgatg agaatgccgg cattaaagca tttttgatgg agcatgcgca
    10381 agaacttcgt ggtgcaatta ttattgacat cgaagccttg ggcgctggcg aactaagctt
    10441 aattaattct gagggcgtta ttagaaaatc gcaaacaagt tcaagaatga aacgctatgt
    10501 tcgaagtgcc gctaacaagc ttggagttgt tatccaaagt gttgatgttc cttggggaat
    10561 gagcagtgcg gcttatgcta gcaagtgtgg atataagact cttcgtcttg ctggtatgga
    10621 tggcaaaaaa cctgccttat acatgtctaa aaacgatgtt gttgaaaatg ttgatgagaa
    10681 caaattaaag gaaaacgtta aatacttgct tcaactaatt caaactattt aattagtttt
    10741 taagctttca ataataaagg tttcgaaatg aagagcacta tttgaggtgc tcttcatatt
    10801 tttttcgcat tcaagagcct tacgaagaat ttcaattagc tcttcctttg tgaaatttct
    10861 tgctcaatag gcatgatttt taactcttca agcctcatat ccaagcgctt gcgaaattgc
    10921 attttgagtt gcaacttgtc ctaaatcttg aacacagatt agctctttaa ctattttgca
    10981 agtttgaggc aagagcatat attcgcttcc agaactcatt tcttgaaata gttgcaagca
    11041 cttatttaga tctcttgcag caaaagcatt tgtaaaatcc caaggttttg attcagctga
    11101 cttttgaact tcgtcattaa cttgttggag tgtgatgttt tgattgccgt ttgataatag
    11161 taacttttga atttctgcat caagctttaa agtatccgga ccaacaagtt caaccaatct
    11221 ttgcgctgca ttcttgtcaa ttcctccgcc gtgacttcga gcaatattgt ttaagctatc
    11281 tgcgacaaga taagttttga cagacgaaca atcaattatc gaagtattgc ttatttttgt
    11341 gcaagcctta aacagacgtg agttctttgc aagcttcgtg caaacaagag ctaaaattgt
    11401 tgttgggtta gggttttcca ggtattcgac aatacgttca gcatcatcct tttttagctt
    11461 gtcagcattt tttacaacaa cgaatctttt ttcacttgcg aatggaattt gcatacacgc
    11521 cgaagtgatt tcagtgccaa ttgctttctc gccatcaaaa gtacttgagt taaaactcat
    11581 gtcaccaaat tcttccatgc gtttggataa gcgctcaagg acaactttag acttcagctt
    11641 gtcatctcca actatcgcat aaaccggcaa caa
//
LOCUS       DBNCDC010000024        11626 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_2151_length_11626_cov_8.030680, whole genome shotgun sequence.
ACCESSION   DBNCDC010000024 DBNCDC010000000
VERSION     DBNCDC010000024.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 11626)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11626)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11626
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2151_length_11626_cov_8.030680"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="AC04VQ_02925"
     CDS             <1..233
                     /locus_tag="AC04VQ_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIO9553155.1"
                     /translation="VFTLNESDAQSFASQGQQRSIVLAFKLSEVKLIEDILDKRPILL
                     LDDVMSELDESRRKKLQQFIEDKTQVFITSTN"
     gene            complement(316..963)
                     /locus_tag="AC04VQ_02930"
     CDS             complement(316..963)
                     /locus_tag="AC04VQ_02930"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIO9553156.1"
                     /translation="MQKHADQLNNFHFASLHFTSEIGTDCTFKLPKTHKWVATAENTS
                     TGGLKFSANMPTEEVFCVPEKYGVNGKIVSEKPVLTKNGLILKPQFEVKDGKIVKFTA
                     EKGQEIMEALINSDKNGAYLGEVAIVPYDTPISRQGITYIHTLFDENAGCHTAVGNAY
                     SKCIEGGLQMTPEELEDAGLNVCKFHEDCVFGTKETKIVGTTGEGEEITIFEHGA"
     gene            complement(1093..1215)
                     /locus_tag="AC04VQ_02935"
     CDS             complement(1093..1215)
                     /locus_tag="AC04VQ_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553157.1"
                     /translation="MDELDVSRQLYVNSWQSSNFQNFKRGLEIPYTICAASNRE"
     gene            1775..2740
                     /gene="guaA"
                     /locus_tag="AC04VQ_02940"
                     /pseudo
     CDS             1775..2740
                     /gene="guaA"
                     /locus_tag="AC04VQ_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013151.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
     gene            2756..3985
                     /gene="brnQ"
                     /locus_tag="AC04VQ_02945"
                     /pseudo
     CDS             2756..3985
                     /gene="brnQ"
                     /locus_tag="AC04VQ_02945"
                     /inference="COORDINATES: protein motif:HMM:NF017347.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
     gene            3995..4201
                     /locus_tag="AC04VQ_02950"
     CDS             3995..4201
                     /locus_tag="AC04VQ_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553158.1"
                     /translation="MLIGIALGFITGRGHDDRKQLKIYRYNLEHADELKKPMVVQPVD
                     VEAERGKKREQRKADRASKKKGKK"
     gene            4201..5481
                     /locus_tag="AC04VQ_02955"
     CDS             4201..5481
                     /locus_tag="AC04VQ_02955"
                     /EC_number="4.4.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015760009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0080146 - L-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cysteine desulfidase family protein"
                     /protein_id="HIO9553159.1"
                     /translation="MDRNSETYKTFCAILEEELVLAQGCTEPISVAFCAVRAREILGE
                     LPEKIRIQASGSIIKNVKSVIVPNTGRLKGLEASAAAGVIAGDSSKKLECIASATEEQ
                     TKQMKEFLDKCEITVEPLYDGATFDMILTLTKGDHTSRVRITHDHTNIVLEEKDGEVL
                     LEKEFDKDDEQYETDRSVLSIQSIWDFITTFNIPDLKKLFDWQIGHNMSIAEEGLAND
                     WGANIGSTILKHRGDKDVRFRAKAYAAAGSDARMNGCEMPVVCNSGSGNQGMTVSNPV
                     VVYAMDMDAPDEKMYRALALSNLVAIHQKDGIGRLSAYCGAVSAAAGAGAGIAYLKTE
                     SYDAVIHALVTTLAVTSGMVCDGAKASCAAKIATSLDMALLSLDLYEDGNSFFAGDGL
                     VTEDVEDFINNIARLGKEGMQDTNKNIIDMMVGV"
     gene            5651..6916
                     /locus_tag="AC04VQ_02960"
     CDS             5651..6916
                     /locus_tag="AC04VQ_02960"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIO9553160.1"
                     /translation="MIVLKNMKILDGHKTMKPVAGKAIIIDDEKIQDIIDEAEVPEGA
                     EAIDLGGHYLMPGLINLHVHLPASGKPTKKKLDYEKLGKLLRFGVVRAVIRKICEGSA
                     KSQLMSGVTTIRTVGGVLDLDSKLRDDINNGEVIGPRILAANTAIGVPGGHMDGSVAK
                     AANSVDEAVEMVRKLDKEKVDLIKLMITGGVLDAEVPGEPGDLKMPPEYVKAVCDEAH
                     KLGYQVAAHVEGPEGMVVALENGVDTIEHGGKPSKEIVELFKKTGAKLICTLSPTIPF
                     TKLSQEVTGFSDLDVQNGSALFNYMVELYNMCLDNQVPIGLGTDTGCPYVSQYDTWRE
                     LYYYCKFCDVTPKLALHSATLLNAQIAGIAEETGSVEVGKSADFMVVKDNPLEDITAL
                     RKPSHVYFRGAEVDTSKLKKYPEIESALN"
     gene            7023..7145
                     /locus_tag="AC04VQ_02965"
     CDS             7023..7145
                     /locus_tag="AC04VQ_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553161.1"
                     /translation="MLSKIQNFGSAMLCPVMFMAFAGVAIGLSVFFTSGTLLGD"
     gene            7188..7676
                     /locus_tag="AC04VQ_02970"
     CDS             7188..7676
                     /locus_tag="AC04VQ_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIO9553162.1"
                     /translation="MSAGGWTVIKQIPLVFAIGLPIGLAKKANARACIEVLITFITFH
                     YFVNTMITTWPELFHNIDLTVDVDSSARLAMIANIPTLNMGVFGALLVSGITVFIHNR
                     YYDKKLPVAISVFSGSVFVYTIAFVVMLPLAFFTSLFWPYVQDAIFGIGSFVNTSGPL
                     GM"
     gene            7734..8168
                     /locus_tag="AC04VQ_02975"
     CDS             7734..8168
                     /locus_tag="AC04VQ_02975"
                     /inference="COORDINATES: protein motif:HMM:NF014437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIO9553163.1"
                     /translation="MPMEYDNLVMDGGITANWLAMLPQLATDPTPIKDVIPEVRFGMT
                     NPSRIFGATGICAAMYVCAKPENRKMVLGVLIPAWVSAVFCAISEPLEFTFLFVAPAL
                     FYVHAALAATMSTLMFELGGCGLLSDSLFHFISQYAALVQLH"
     gene            8241..8603
                     /locus_tag="AC04VQ_02980"
     CDS             8241..8603
                     /locus_tag="AC04VQ_02980"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIO9553164.1"
                     /translation="MKKFNVPTPGRTDEAEMRLRTKKEYRDHQNNKENDKAKSLDSTD
                     FVAAIEEGVGGRDNIDYLTNCISRLRITVKDTNKLKDDAYFKEIGAKGAVKVDKTIQI
                     IIGTDVPQVRTKFEEYID"
     gene            8806..11310
                     /locus_tag="AC04VQ_02985"
                     /pseudo
     CDS             8806..11310
                     /locus_tag="AC04VQ_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012799454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
     gene            11310..>11626
                     /locus_tag="AC04VQ_02990"
     CDS             11310..>11626
                     /locus_tag="AC04VQ_02990"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF017493.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HIO9553165.1"
                     /translation="MKIIEASTIAELVRKGIPHLAYELPVDISAGQCAMCEHERIPIG
                     KSVLSQLCTNDKIAREDQVPLCQDTGTVRVSIQVGKDVAISGDVFDGVNDAVAEAYDK
                     GKLR"
BASE COUNT         3097 a         2416 c         2862 g         3251 t
ORIGIN      
        1 tcgttttcac cttaaacgaa agtgacgccc agagcttcgc cagtcaaggt caacaacgaa
       61 gcatcgtcct cgcttttaaa cttagtgaag ttaaactcat cgaagacatt ttggacaagc
      121 gaccgattct tttacttgac gatgttatga gcgaacttga cgaatcgcgc cgcaaaaagt
      181 tgcagcaatt catcgaagac aaaactcaag tcttcattac ttccacaaat taaaatcgag
      241 taaattccac gagtaaatcg agtaaatgcc acgagtaaat cgagtgtggg ccccggggaa
      301 atcgagttag atggttcagg cgccgtgttc gaagatggtg atttcttcgc cttcgcccgt
      361 tgtgccaaca attttggttt ctttagtacc gaagacacag tcttcgtgga acttgcaaac
      421 gtttaggccg gcgtcttcga gctcttcggg ggtcatttgc aaaccacctt cgatgcattt
      481 tgaatatgca ttaccaacag cagtgtggca tccagcattt tcatcgaaga gcgtgtggat
      541 gtaggtgatc ccttggcgcg aaattggagt gtcataaggc acaattgcaa cttcgccgag
      601 gtaagccccg tttttgtcgg aatttataag cgcctccata atttcttggc ctttttcagc
      661 tgtgaatttg acaatcttgc catccttaac ttcaaattgt ggctttaaga tgaggccatt
      721 ttttgttaga acgggctttt cgcttacgat cttgccgtta actccgtatt tttccggaac
      781 gcaaaagacc tcttcggtag gcatatttgc gctgaatttc aagccgccag tgcttgtgtt
      841 ttcggcagtg gcaacccatt tgtgagtttt tggaagcttg aaagtgcaat cggttccaat
      901 ttctgatgtg aagtgtagcg atgcgaagtg aaaattattt aactgatcag cgtgtttttg
      961 catttcatca cagtggacga atcattcctt gacaggatca gttttgccat caattcgagc
     1021 aacctcgaaa aggttctccc aaactttgat gtaggcttcg tctgggtcaa gatccgggaa
     1081 gacgcatcgc gctcattcac ggttcgaagc agcgcaaatt gtgtatggaa tttcaagacc
     1141 acgcttgaag ttttgaaaat tcgaactctg ccaagagttt acgtaaagct gtctgctgac
     1201 atctagttca tccataaaat ctggtccatt gcaaaataca ataacgaagg ccaggtcctt
     1261 gtttccatat ttttcgcgac agcggtccgg ccaaggctgg cgattttcaa ggaattgttc
     1321 ttcgctgtga gcgtaatttt cgcgatcggt gtaggggtca gctcagtaaa cggtgacgtc
     1381 gcgagcgcct tgcttgtatg cttcgcgagt tagtgcgcga gcaagatcag ggcaggtgat
     1441 agggcatgaa attccaagag ctcaaccttt ttgaaaacga attccgccat taattatcca
     1501 cgaagcgtag gcctcaatct tctcttcgaa attttcaatc ccgtccacta aaactttgtc
     1561 atacatgtag tctttcataa ttcctcccaa aaggtgtatt tcatgcctgg caataattac
     1621 accttttgat gtatttcatg cctggcaata attacacctt ttattataca ctatgtgtgt
     1681 tagagaggag ggattgtggt aaaatgcagt gcattatcag gaggtaaaaa tgacaacacc
     1741 aaattctact caatatgtta aaagaccaga tgagatggct cgaatcgact cgctcgaact
     1801 tgcgaacgcc ttcgtggatg agcaagttga gcatattcgc aagcaagttg gaagtgatcg
     1861 agtgcttctc gcgctatctg gtggtgttga cagttctgtt gttgctgcgc ttcttatgaa
     1921 ggcgatcggc cagcagctgg tttgcgttca tgtaaatcac ggccttatgc gtaagggcga
     1981 aagtgagcag gtcatcaagg tttttaagga tgagctcggc gcgaacctgg tttatgttga
     2041 tgcgaccgac cgcttcctcg acaagcttgc tgacgtcgat gagccggaaa ccaagcgcaa
     2101 gatcattggc gcagaattca tccgcatttt tgaagaagaa gctcgcaagg ctgccgaaga
     2161 cgaggccgcc ggcggtatca aatttttggc gcaaggcaca atctatcctg acattttgga
     2221 aagtgacggt gttaaagccc accataatgt tggaggcctg ccggatgatt tgcaattcga
     2281 gcttgttgag ccggttgcaa ttttgttcaa ggacgaagtt cgccaagttg gacttgccct
     2341 tggccttcca gaaagtatgg ttttccgtca gcctttccca ggtccaggct tgggtgttcg
     2401 atgcctcggc gctatcactc gcgacaggct tgaagcgctt cgcgaagcgg atgcgatcct
     2461 tcgcgaagaa attgcaaacg ctggactcga caaagaaatt tggcaatatt ttgtaagcgt
     2521 tccggatttt cgaggcactg gcgttcgcga cggcaagcgc gcctacgatt ggccggcgat
     2581 aattcgttgt gttaatacga ccgatgcgat ggaagtatca atcccgcgtg ttgattgaga
     2641 agttttggaa aaaatcacaa accgcatttt ggacgaagtt ccaggaattt gcagggtttg
     2701 ctacgacctt tctccaaagc cccctgccac tattgagtga gaataaagga gaatcatgaa
     2761 acttagaatt cgcgatgtct tgaccctcgg gcttgcgtta ttcgcaatgt tttttgggtc
     2821 aggaaacgta attttcccac catttttagg tatgcaaact gcgccgaatt ggtttgtcgc
     2881 aatttttgct taccttgcgg tcgatattat catcgcagta ttttcgatca tcgtcatggc
     2941 aaaaaatgac gaaggcctaa ctggcatcac gcagcgcatc ggcaaagtcc catcgcgtat
     3001 cgtgggtatt gcaattattt tgtgtatcgg tccgctcatc tgcattccgc gtgcggccgc
     3061 tgtttcatac gatttgctca tggttccgct ctttaacttg ccgaacaacg gctgggaaca
     3121 aattctgttc tcgtttatat ttttctttgt cacttggtgc ttggttgttc gcccatcaaa
     3181 aattgtcgac atcgttggca acattctcac gccgctttta cttgtaacat tggcgatgct
     3241 aattgtcaaa ggcttgctcg atccagctgg cgaagtccca gcaacccctg tcatcgataa
     3301 cgctgtcggc gagggaatta tcgcgggcta ccaaagtatg gatttgtttg gcgcgctgag
     3361 cttcggcact atcatgatta caacttcgtt tatcaaaggt tacaaaactc cgaaccatcg
     3421 cgtttatgtg cttacttgag caggtatttt gtcgctcgtt ttgctctttg cgacatcaat
     3481 cggtatggct tatttgggtt cgcaagttgc aaatgaattt actgacatca caagtcaagc
     3541 ggttcttgtt tcaaaaattg catcgaaggc atttagcttc ggcggaattt tgcttggtgt
     3601 gcttgcaagt ttagcatgtc ttacaactgc agttggactg acgtctggaa cttcggtcta
     3661 cttcaaaaac tttgttgaca aacgcgatca gcgtggaaaa gcatatgaga ttttgtgcac
     3721 cgttgtttgt tgcgtaagtt ttgtaatttc gaaccttggt cttgaattga ttgttgaatg
     3781 agcctcgcca gttttgactt tggtattccc gatggcggtc gttcttgtta tcctttcgtg
     3841 gttcaatacg aagatcaaga gcaatcgcgt ttatatgttt gcggcgctgt tcgccttcgt
     3901 ttcgaatttc tttgttgtga ttagcgagtg gttctacgtt ccttatattg atcttttgcc
     3961 gcttgctgaa atgcagctgg gttgagttat tccagtgctt attggtattg cgcttggttt
     4021 tatcacaggc cgaggccacg atgaccgtaa gcaactcaaa atttacagat acaatttgga
     4081 acatgccgac gaactcaaga agccgatggt tgttcaacca gtcgatgttg aggccgaacg
     4141 tggaaagaag cgtgaacaaa gaaaagcaga ccgtgcttcg aagaagaaag gtaagaaata
     4201 atggacagaa attcagaaac ttacaaaact ttttgtgcga ttttagaaga ggaattggtt
     4261 ttggcgcaag gctgcactga gccaatttca gttgcgtttt gcgcggtgcg tgcccgtgaa
     4321 attttgggcg agctgcctga aaaaattcgt attcaagcaa gtggtagcat tattaaaaat
     4381 gtaaagagcg taattgttcc aaatactggg cgtttgaagg gcctcgaagc ttctgctgcg
     4441 gctggcgtta ttgctggcga tagctctaaa aagttggagt gcattgcctc ggcgaccgaa
     4501 gagcaaacca agcaaatgaa agaatttttg gacaaatgcg aaatcacggt tgagcctctc
     4561 tatgatggcg cgaccttcga tatgattttg acgctcacga agggcgacca tacttcgcgc
     4621 gttcgcatca ctcacgacca cactaatata gtgcttgagg aaaaagacgg cgaagtttta
     4681 ctcgaaaagg aatttgacaa agacgatgag caatacgaaa ccgatcgaag tgttctttca
     4741 atccaaagca tttgggattt tattactacg ttcaacattc cggatctcaa gaagcttttt
     4801 gattggcaaa ttggtcacaa catgagcatt gccgaagaag gcctcgccaa tgattggggt
     4861 gctaacatcg gtagcacaat tttaaaacac cgaggcgata aagacgtgcg ttttcgtgcc
     4921 aaggcttatg cagccgctgg ttctgatgcg cgaatgaatg gttgcgagat gccagttgtt
     4981 tgcaattctg gaagtggcaa tcaaggcatg acagtttcga accctgttgt ggtctacgcg
     5041 atggacatgg atgcgccgga cgaaaaaatg tatcgtgcgc ttgcgttatc aaatcttgtt
     5101 gcaattcacc aaaaggacgg aattggccga cttagcgctt attgcggagc tgtttctgcc
     5161 gctgcgggcg ctggcgcggg catcgcttat ctaaaaaccg aaagttacga tgctgtgatc
     5221 cacgcacttg ttacaacctt ggctgtaact tcggggatgg tttgcgatgg agcgaaggct
     5281 tcgtgtgcag caaagattgc aacttcctta gacatggcac ttttgagttt ggatctctac
     5341 gaagacggca actccttctt cgcgggtgat ggactggtta ccgaagatgt tgaggacttc
     5401 attaacaaca tcgcgcgcct cggcaaagaa ggcatgcaag acacaaataa aaacataata
     5461 gatatgatgg taggagtata gtagcaaatt tgcctaacgg caaatttagc ttcgttacgc
     5521 tgcgaaatat taatttttat atctttgcct ttgttttgct actcgcgtat aatatacgcg
     5581 tcgtagcaaa ggcaggcaaa tatattaaaa attaattatt tctcgctgct tatacgaata
     5641 tggaggattt atgattgtat taaaaaatat gaagatttta gatggtcata agactatgaa
     5701 gccggttgct ggcaaggcca taattattga cgacgaaaaa attcaagaca ttatcgacga
     5761 agccgaagtc cccgaaggcg cggaggcaat cgaccttggc ggtcattact tgatgccggg
     5821 tctaattaat ctccatgttc atcttcccgc aagtggcaag cctactaaaa agaagcttga
     5881 ttatgaaaag ttggggaaac ttcttcgctt cggagttgtg cgtgcggtaa ttcgtaaaat
     5941 ttgcgaaggt agtgcgaaga gccagctaat gtctggagtc actaccatcc gaacggttgg
     6001 cggcgtgctg gatttggact ctaaacttcg tgatgatatc aacaacggcg aggtgattgg
     6061 gcctcgaatt cttgcggcca acactgcaat tggtgttccg ggtgggcaca tggatggctc
     6121 ggttgctaag gcagcgaaca gtgttgacga agccgttgaa atggtccgca agcttgacaa
     6181 ggaaaaagtt gacctaatca agctcatgat tactggtggc gttttggatg ccgaagtccc
     6241 aggtgagccc ggtgatttga agatgccacc tgaatacgta aaagcagttt gtgacgaagc
     6301 gcacaagctc ggctatcaag ttgcggctca tgttgaaggc cccgaaggga tggtcgtagc
     6361 gctcgaaaat ggagttgata caatcgagca cggtggcaag ccttcgaaag aaattgtcga
     6421 gcttttcaaa aaaactggcg cgaagctgat ttgcaccctg tctccaacaa ttccatttac
     6481 aaagctgagt caggaagtta ccgggttttc ggacctcgat gtgcaaaacg gatctgcgct
     6541 tttcaattat atggttgagc tttataacat gtgtttggat aatcaagtgc ctattggact
     6601 tgggaccgac acaggctgcc cttatgtttc tcaatatgat acttggcgcg agctctacta
     6661 ttattgtaag ttctgcgatg taactccgaa gctggcgctc cattccgcaa ctttgctcaa
     6721 cgcgcaaatt gcaggcatcg ccgaagaaac tggctctgtt gaagttggca agtctgcaga
     6781 tttcatggtt gttaaagata acccgttgga agacattacg gcacttcgta agccttcgca
     6841 cgtttacttc cgaggcgcag aagtcgacac ttccaaactc aaaaaatacc cagaaatcga
     6901 atctgcatta aattaattcc ccggggccca cactcgattt actcgtggca tttactcgat
     6961 ttccccgggg ccagtactcg attttggaaa atagaaattt aaaaataagg gaaaggtaat
     7021 ctatgttgtc aaaaattcaa aactttggtt cagcaatgct atgccctgtc atgtttatgg
     7081 cttttgctgg tgttgcgatt ggtctttcgg tgttcttcac aagtggaacg cttttaggtg
     7141 attgagcttc tgatgaaaat ggactatggt ttcaatgttg aaacgttatt agtgcgggag
     7201 gttggactgt tattaaacag atacctcttg tttttgctat tggtctgcca attggcttgg
     7261 ctaaaaaagc aaatgcccgt gcatgcatcg aagtgctgat aaccttcatc acgtttcact
     7321 attttgtaaa cacgatgatc acaacatggc cagaactgtt ccacaacatt gatcttacag
     7381 ttgacgtcga ttcttcggca cggctagcca tgattgcaaa tattcctaca cttaacatgg
     7441 gtgtttttgg agcgcttctg gtttctggaa ttacagtttt cattcacaat agatattatg
     7501 ataaaaagtt gcctgtggct atttcggttt ttagtggttc agtgttcgtt tatacaattg
     7561 catttgttgt tatgttgcca ctcgcatttt ttacttcgct gttttggcct tatgttcaag
     7621 acgctatctt cggaattgga tcatttgtaa atacgtccgg tccgctgggc atgtgaatat
     7681 atattttcct cgagaagggg ctgctaccat ttggaattca tagcatgctt tgaatgccga
     7741 tggaatacga caatcttgtt atggatggcg gaatcactgc aaactggctt gctatgcttc
     7801 ctcaacttgc tactgatcct acacctatta aagatgttat tccggaggtg agattcggca
     7861 tgacaaatcc gtcgcgaatt tttggagcca ctggaatttg tgcagctatg tatgtttgcg
     7921 ctaagccaga aaacagaaaa atggtacttg gcgtgctaat tcctgcatgg gtttcagcag
     7981 tattttgtgc gatttcagaa ccgttggaat tcactttcct gtttgttgcg ccagctctgt
     8041 tctatgttca cgcagctctt gcggcaacaa tgtcgacttt gatgtttgaa cttggaggct
     8101 gcggtcttct aagcgacagt ttattccact ttataagtca gtatgcggca ctggtccaac
     8161 ttcactgacg agaaattttg ataattgttg ttgtgggtct tgcctttatg gtcatttact
     8221 tttttgtgtt taccgcttga attaagaagt ttaacgttcc aactcctggt cgaactgacg
     8281 aagcggaaat gcgtcttcgc accaaaaagg agtatagaga ccaccaaaat aataaggaaa
     8341 atgacaaggc aaaaagttta gacagtaccg attttgtagc ggctatcgaa gaaggggtcg
     8401 gcggccgtga caatattgac tatttaacta attgtatatc gaggctacga atcactgtta
     8461 aagatacaaa caaattgaaa gatgatgctt actttaaaga gattggtgct aaaggtgctg
     8521 taaaagttga taaaactatt cagattatta taggcaccga tgttcctcaa gtccgcacaa
     8581 aattcgagga atacatcgat tgagaataat tgccacgagt aaatcgagtt ctggccccgg
     8641 ggaaatcgag taaatgccac gagtaaatcg agcgtgggcc ccggggaaaa tgtggtagaa
     8701 tgggagcgct aaatgtgggg gttgccccac ggctagataa gggaaaagca atcgggggat
     8761 tgcacgggag agcaaacgct cgccccctag cagaagagag gtattatgaa acttgtagtt
     8821 accgaaaagc aagatgccgc gcaaaaaatt gcgaacattc ttggcacaaa agtcaaatca
     8881 gacaaagttt tcacaacgcc ggtttatcgc ttcgaagttg atggcgaaga atgggttacc
     8941 atcggacttc gcggtcacat tttggagccg gatttcccaa aaagtctaat ttacaacaaa
     9001 agtgctggtt gacatggcga agacgaggac ggcaccaagt atgatgctaa cattccaagc
     9061 aacttgccag cgccgccttt cgacaaaaag cgcaagcctt ttctgaaaga tggaatcgat
     9121 ttaaagtctt ggaagcttga agcgcttccg tatttgactt attcgccggt tcataaaaat
     9181 ccgaaagaaa aggcaatcat tcgctcgctt aaaaatttag cgaagaaggc agacagcgtt
     9241 gtaattgcaa ctgactttga tcgcgaagga gagttgattg gttccgacgc gctgatgtgt
     9301 atccaagaag ttaatgacaa agccccagtt agcagggcta gatattcggc atttacgaag
     9361 gaagagatcg aaggcgcgtt caacaatttg gtcgagcttg attataactt ggccgatgct
     9421 ggcggaactc gccaagacat cgatttaatt tgaggtgcgg tccttactcg ctatttgacg
     9481 cttgttaaat ttgcgggtta tggaaatgtt cgaagctctg gtcgcgttca aactccaact
     9541 ttgtctttgg ttgttgaaaa agagcgcgag cgcatggctc atgttcctga aaaatattgg
     9601 gtaattgacg ggagctttgg agagcctggc cctgaaccag aagaagagga cgacaagaaa
     9661 aagaaggatg aaaacccact tccacgtggt gcttttgagg cagtttataa gactgctcgt
     9721 gttaaggata agggcgaagc agatagcgtt atgagtgcaa ttgaaggagc gaaggaagcc
     9781 aaggtttctg ctgctgataa aaaatcgcga agcgttgcag ctccaactcc atttaataca
     9841 acatcgctta tggctgcggc tgcaagcgaa ggcttatcgc ccgcgcgaac aatgcgaatc
     9901 gctgaaagtc tttacatgaa tggttatatt tcttacccgc gtgtagataa cacagtgtat
     9961 ccggcttcgt tgaatttagc tgaaacagtt agtaagctaa agaagaattc ggattacgct
    10021 ccttattgtg agaagttgct cgctcaagga aagttgactg ctaccagggg taagaaggag
    10081 acaaccgatc acccgccaat ttatccaacg gctggtgtta atcctggaga attggccccg
    10141 gctgattata agctttacaa tttaattgca cgaagatttt tagcgacgct ttcgggtccg
    10201 gcaacaatgt tgggaacaaa aatttcgctc gacgttaatg gtcaagagtt tgttgctaag
    10261 ggcgacatcc tcgataagcc aggcttccgc gaaatttatc cgtttggtct caagaaagat
    10321 gagcagcttc cggatatgaa agttggcgac acaatttctt tcaatggcgc gcgctgcacg
    10381 gaaaaagaaa ctgaaccgcc agcgcgttat agccagggtc gtctaattca acagatggag
    10441 aagcttggcc tcggaactaa aagtactcgt cactcaatta ttgagcgact ttacactgtt
    10501 aaatatttcc agaacgatcc aatcgagcct tcgcagcttg gaattgcggt ttgcgatgcg
    10561 ttggaaaaat atgcgccggc cattacgcat ccagaaatga ctgcgcaact cgaagcagaa
    10621 atggatgata ttgccgaagg caggaacacc cgaacttcgg tagttgaaga ttcgcgaaat
    10681 aatttgtccg gcgagcttga aaacttgctt ccgcacgctg atgaaatcgg cgaagccctc
    10741 tccgacgctg tggccgccga tgcttatgtt ggaaaatgtc caaagtgtgg taaggatctc
    10801 cagcttcgtg catctgcgaa gacacgcagc atgtttattg gttgtgctgg ttggccggat
    10861 tgtgatgtta catacccgct tccaagtgga aagattgaag ctttgtctga gccttgtgag
    10921 aaatgcggga tgcctcaggt caaggttacg gcgtttaggc aaaagcctcg cacactttgc
    10981 atcgatccaa attgcgaaac caaccaagag gcagatgttg ttgttggcat gtgtccagaa
    11041 tgtcaaaagc aaggtaagga agccgagctc atcgctcata aaaatccgcg tacacttaag
    11101 cgattcattc gttgtgaaaa ttttgacgat tgcggtgtgg gctatccgct tcctcaatat
    11161 ggcgagatcg aagcaactgg tcgcacctgc gaacattgcg gtgcgccaaa agttattatt
    11221 catactcaac gtggtccatg agaattgtgt ccaaacttct catgcccaga gaaaaaattg
    11281 aaagacgaaa aaaagaaagg aaagaagtaa tgaaaattat tgaagcttct acaattgcag
    11341 agttggtccg aaagggaatt ccgcatttag catatgaatt gccggttgac atttctgctg
    11401 gtcaatgtgc tatgtgcgag catgagcgca ttccaattgg gaagagcgtg cttagtcaac
    11461 tttgcacaaa tgacaaaatt gctcgcgaag atcaggttcc gctttgtcag gatactggaa
    11521 cagttcgcgt ttcaattcaa gttggtaaag atgttgcaat ttcgggcgat gtcttcgatg
    11581 gcgtaaacga tgctgttgct gaggcttatg ataaaggcaa gcttcg
//
LOCUS       DBNCDC010000025        11623 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_2153_length_11623_cov_7.094917, whole genome shotgun sequence.
ACCESSION   DBNCDC010000025 DBNCDC010000000
VERSION     DBNCDC010000025.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 11623)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11623)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11623
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2153_length_11623_cov_7.094917"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(1..74)
                     /locus_tag="AC04VQ_02995"
     tRNA            complement(1..74)
                     /locus_tag="AC04VQ_02995"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40..42),aa:Gly,seq:tcc)
     gene            complement(78..154)
                     /locus_tag="AC04VQ_03000"
     tRNA            complement(78..154)
                     /locus_tag="AC04VQ_03000"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(118..120),aa:Pro,seq:tgg)
     gene            584..1852
                     /locus_tag="AC04VQ_03005"
     CDS             584..1852
                     /locus_tag="AC04VQ_03005"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIO9553166.1"
                     /translation="MVGRFFAKLGHWIAIPFVFLWKLIVKGVRKFFQLDDDTAPNLVD
                     VKKEKKGLRRLDNRQRAMRNRAFVVAAVVIICPLVIMGGFKVAGALELNGGFAGQTGD
                     TYFKEADVTKYITEQTLYKSYGKSQSQWQQYLSMYGMTVEKFRQQCIEDKFQHDEMVR
                     RACDQEGITVDEDQVDQHVASFRAKYDTDANYKKALENAGYTEETYRDNVKQTMLEKA
                     LIEKVITVDAPSDDDVKTYLSSYSSTYKDARQSSHILFDAKDSATAQSVLDQINAGTL
                     SFDAAVAQYSKDEATKAKGGDRGWDKIESFTSQYNSGLSGLSAGEVCKELVTDSEGIH
                     IIKCTAKWDTPEKIESLDGVPQAIIEECRKKKNESNQATELKTWLETFASDNGIENKV
                     FPMPNGLPYYTEVSASSSSAATSAATTSTG"
     gene            1940..2239
                     /locus_tag="AC04VQ_03010"
     CDS             1940..2239
                     /locus_tag="AC04VQ_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIO9553167.1"
                     /translation="MEFEISKSAEKTTVKVKGKIDSATVYELEEGIKEEMLLAKTMAI
                     DFSELEYISSAGARLLLMIQKKMNKRQGNLVIMNCNKDVLDVFEKTGFTQVLTII"
     gene            2255..4924
                     /locus_tag="AC04VQ_03015"
     CDS             2255..4924
                     /locus_tag="AC04VQ_03015"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIO9553168.1"
                     /translation="MPLVYAGLITTVCTVILFALEKKTKFGRWNPILRQVIIGVVFGL
                     CAAYATEIGSFNVNGAALNCRDAGPVIAGLMFGPWSGVIAGVIGAFDRFFIATPIFGI
                     GAYTWIPCSVATLCAGIFAGVFHEIMFKDHRPFVLHALVFIMLTEVFHMGMVPLFHMD
                     DMQNATMVMYNVFLPLLLSNAAVVGASFFITSFMDMRFKGHKVREIIFSKDIFFTQDL
                     FFSFQFWLLVIICFAFATNIIAMSFFNDRMSVASTETRFKSDLTSMQKLIVQDAETLQ
                     VSHVGMAKVYARDWSDEEVSQYFIVNTATGEILSSERGVPELYQECTTKPELDLFETN
                     INGTHYYVDYVNLEDFTIVQYSIEKYEMFIPHLNSSFTMFLQIVVFGVMMFSVFMLLK
                     KLVGENLLKINESLEQITSGNLDTKVDVYSHKEFAALSDRINATVDTLKDYIQAEADR
                     FKEDFEMARQIQLSALPNVFPPFPDNKEIDLHAKYQSAKEVGGDFYDYYFVNKNTLAF
                     LIADVSGKGIPAAMFMMRAKTAIRVFAEKHLDAAKTLEATNDYLNENNEAELFVTCWL
                     GFINTETGLMQYATAGHNPPVVINEETGKCEFLDAPISLVLGGMEGIKYNNAEYQLQP
                     GEKLLLYTDGITEAHNEKSELYGDDTVVDFAKKNIKADVTTLCDNLFQDVENFAGEAD
                     QFDDETVLAFIYNGKPKPKVENLFETEISVFAKTENVTRVCDRLEEILPQTNAPLDFT
                     PKFCVAIDEIFSNIVKYGYKDPSKYVTVRVQVDERDPKKKAVKVTFVDAADRFNPLDE
                     SAPDITLSAEEREIGGLGIYMVRKLMDDVFYIYKDKANNLTLVKYFGEGAPSKEEIQK
                     MQEEENKKQEAESAAKKEPKPKPKEDKPPTDDK"
     gene            complement(5601..6476)
                     /locus_tag="AC04VQ_03020"
     CDS             complement(5601..6476)
                     /locus_tag="AC04VQ_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553169.1"
                     /translation="MNIFISNFTGIEFYLGARSNNYLEILTIRGNNILGANIYTPNLY
                     KYQLTESNVSHNNNLTIRTNDITKSYIPYVDTTKSVDVIVSKSSNQNKTKLVNYHLIS
                     GKLPPYSFLKVAKGLYVVCPELLFCQMASVFSPVRLLLFGLELCGTYAMRHEEDNSFM
                     PNVPALTTAERIKKYINSLQKCQAHFRGIAKAKNIAQYLSNNSASPQESKLYVQLCAP
                     RKLGGYGIRNIKFNQNIQLSKAASNIIGYSKIRPDLCNPKTKLVIEYDSTYYHENADQ
                     NKHDKLRLDAFHNDG"
     gene            6616..7374
                     /locus_tag="AC04VQ_03025"
     CDS             6616..7374
                     /locus_tag="AC04VQ_03025"
                     /inference="COORDINATES: protein motif:HMM:NF013703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIO9553170.1"
                     /translation="MKIFKKNNDTSQQVKDFRAAEAEKRRSITKQTFIGNLRTMSVSI
                     LAITLAMVIDGIIIGQFLGTDAIAGYGLASPIFMIINAIFAVFGTGAQSVCANFMGRG
                     DMKKANGIFNATLIATLALSALMTLVLIIALDPLAQMLCATNSGDNAEALVQGTKDYI
                     LGITVGIPIQFLCRVISPFMQLDSDRQRVLPSTLSGSGINIAGDLFNALVLNWGLFGM
                     ALASSVGFCGELTILLLHFRKKTAMYKINFKLSS"
     gene            7420..7929
                     /locus_tag="AC04VQ_03030"
                     /pseudo
     CDS             7420..7929
                     /locus_tag="AC04VQ_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013703.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
     gene            7930..8445
                     /locus_tag="AC04VQ_03035"
     CDS             7930..8445
                     /locus_tag="AC04VQ_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIO9553171.1"
                     /translation="MSFIACEILMVLVYTIIVMVRKKSPKIKLDDYLLLPKEYDELES
                     NNFEFTIHDEEDVMMSSVGAEAFCIKNNIDEKRTNAVALCIEEMGANIVENGIREGEK
                     LFLDIKVILRGEDIVIRLRDNCSDFNPVDHHKTDSDDPFAGIGIKMVFKMAKDVNYIM
                     TMNLNNLVITI"
     gene            8459..9319
                     /locus_tag="AC04VQ_03040"
     CDS             8459..9319
                     /locus_tag="AC04VQ_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIO9553172.1"
                     /translation="MKSKFEKGTLTVKFGVKNASGSFLAKFDKFMNEEIRKHKPKKIV
                     FDMDGVEKVTSAILRRFLRIINFGFEEEIINASKSVVEILTITNIDKMIHVVHKRPLI
                     SIKNLKEISRGGYGVVYEYSKDEILKVYNGHLPEEQILRGVKNSKVAFQRGLSVAIPY
                     ATVDTEEGIGVLFEKVKGTALADVIHNHPERVEPKIKRLVELSKEMAATEFKPGELTS
                     VRDIYINALHSVRKQLPPAYIDAYKLAIESLPDVNRAVHGDLTCVNVLDDGFDLIMVD
                     MDTFSFCHPI"
     gene            9512..9661
                     /locus_tag="AC04VQ_03045"
     CDS             9512..9661
                     /locus_tag="AC04VQ_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553173.1"
                     /translation="MRTAALYSQNIDEFDKNNPRHVEFLGYIKAILENSVLKNLYSMV
                     EIFNT"
     gene            9669..10397
                     /locus_tag="AC04VQ_03050"
     CDS             9669..10397
                     /locus_tag="AC04VQ_03050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIO9553174.1"
                     /translation="MSNCKYKISVITPLHNEAADIFAPAYNSMLKQTIGFKNIQWIIV
                     LHNCDVKTTNYVTRKTKNFKNVCLKILNNEVHTPSSPKNYAMQFAEAKYIAYLDADDW
                     YLPDGLKVAYEQAEKENASIVSFRRQHSFQEPGLYPLMEITAWNQAEDKIVCTQKNVN
                     TKNLYSGQFYFLTSKLFRKSMLIKNKIQFEEDMPLGEDFVFAIEAFANASKIIILPRF
                     IGYHYFIRSNSMAQKNLSSDEAFL"
     gene            10773..>11623
                     /locus_tag="AC04VQ_03055"
     CDS             10773..>11623
                     /locus_tag="AC04VQ_03055"
                     /inference="COORDINATES: protein motif:HMM:NF015291.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553175.1"
                     /translation="MQTLDEFHKTVPPTTRARLDETLNLQTKIGDSGILVNGKPWQYL
                     RSNCGELFMNTSAQNEVFTSALANICADKPTFFVNSGKMQTDHSSNDNVEIKSLESVL
                     CDNAFLFAHKNNCDNFIFDFAKAKSKKNLKQIFALDTQCLVEAFENFEKQNKNLFKKA
                     GRKPIAKYLWPNLEAVIATKKSTNVKLNAKLKKYFGNVHFSNALYFTEEGIIGKRVAG
                     KQNLYEIFDDKNVYELKNCQTNKICNFSNCKEGSNYYLVITNNAGLYRYVSSHKIQIK
                     QKNKFKIT"
BASE COUNT         3572 a         2357 c         2428 g         3266 t
ORIGIN      
        1 tggagcgggc gacgggaatc gaacccgcgt cacgagcttg gaaggctcgg gttctaccat
       61 tgaaccacgc ccgcaaatgg tcgggacgac aggattcgaa cctgcgacat cctggtccca
      121 aaccaggcgc tctaccaaac tgagccacgt cccgtgcgag cgcatattat acaccaaaaa
      181 ataaaaattg tatctaattt atcatctcgg caaaattgca ggaaagtttg ataaaatagc
      241 agacatctac gtgaaaaaat tgtgtcaagg ataaaaaatg gctaagtgaa aattaaaaga
      301 aaaaccaaaa gaggaagtca agaagactaa taaattcatc cctcagaaga aggaaaaaac
      361 tgccgaagag cttgaggcca tcgaggctaa gaaggccaag caggccgaag ccaaggcaaa
      421 gcgcgctgca gagaaggcca aaaagaaggt cgaatctgac aagaagaagg ctgaaaatgc
      481 tgcaaagcgc gctgcgaaaa agaaggtcac tgataagaaa aagaaggaac gcgcaaagaa
      541 gcgtgagaag ttttactcga aaggctttgg aaaagttttg gcaatggttg gcagattctt
      601 cgccaagctt ggacactgga ttgcaattcc atttgtattt ttgtggaagt tgattgtcaa
      661 aggcgttcgc aagtttttcc aacttgacga tgacaccgca cctaatttgg ttgacgtcaa
      721 gaaggagaag aaggggcttc gccgattaga taataggcag cgtgcaatga gaaatcgtgc
      781 gtttgtggtt gctgcagttg taattatttg ccctcttgtg attatgggcg gattcaaagt
      841 tgcaggcgcg cttgagctga acggtggctt cgctggccag acaggcgaca catatttcaa
      901 agaagccgac gtcacaaaat acatcaccga gcaaacgctc tacaaatcat atggcaagag
      961 ccaatcgcag tggcagcaat accttagcat gtatggcatg actgttgaga agttccgtca
     1021 gcaatgcatc gaggacaagt ttcagcacga tgaaatggtc cgccgcgctt gcgaccagga
     1081 aggcatcaca gttgacgaag accaggtcga ccagcacgtt gcaagcttcc gcgcgaaata
     1141 tgacaccgac gccaattaca aaaaggcgct cgaaaatgcg ggctacaccg aagaaaccta
     1201 ccgcgacaac gttaagcaaa caatgcttga aaaagcgcta attgaaaagg tcatcaccgt
     1261 tgatgctcca agcgatgacg atgtcaagac ttacctcagc agttattctt caacctataa
     1321 ggacgcgcgt caaagttcgc acatcctctt cgatgcgaag gacagcgcaa ccgctcaaag
     1381 tgtgcttgac caaatcaacg ctggcacgct cagcttcgac gccgctgttg cccagtattc
     1441 gaaggacgaa gcaacaaagg cgaagggtgg cgaccgcggt tgggataaaa tcgagagctt
     1501 cacttcgcaa tataacagtg gcctaagtgg tctttcagct ggcgaagttt gcaaggagct
     1561 tgtaaccgat agcgaaggca ttcacattat taaatgcaca gccaagtggg atacgccgga
     1621 gaagatcgag agcctagatg gcgttccgca ggcgattatc gaagagtgcc gcaagaagaa
     1681 gaatgaaagc aaccaagcca ctgaacttaa aacttggctt gaaacttttg catccgacaa
     1741 tggcattgaa aacaaagtat tcccaatgcc aaatggtttg ccatattaca ctgaagtaag
     1801 tgctagcagc tcatccgcag caacttccgc cgctaccact tcaaccggct aagatgtaat
     1861 tattgccagg catgaaatgc accaaaaggt gtaattattg ccaggcatga aatacacctt
     1921 ttgagagatt ggaagaaaga tggaatttga aatttcgaaa agtgcggaaa aaacgaccgt
     1981 aaaagtcaaa ggaaaaattg attcggcaac agtctacgaa ctagaagaag gcatcaagga
     2041 agaaatgctt ctcgcaaaaa caatggcaat cgatttcagc gaactcgaat acatttcaag
     2101 tgcgggcgcg cgtctgcttc tcatgattca aaagaaaatg aacaagcgcc aaggcaacct
     2161 cgttattatg aattgtaaca aggacgtcct cgacgttttc gaaaaaacgg gctttacaca
     2221 agtcctgact ataatctaac cgaagggagc cggtatgcca ttagtttatg caggattaat
     2281 caccacagtt tgtactgtta ttctttttgc cctcgagaaa aagacgaagt tcgggcgctg
     2341 gaacccgatt ttacgccagg tcataattgg tgttgttttc ggactttgcg cggcttatgc
     2401 aaccgagatc ggaagtttta acgtcaacgg cgcagcgctt aactgccgcg acgcaggccc
     2461 ggttattgct ggtctaatgt ttggcccgtg gtctggcgtt attgccggtg tcatcggtgc
     2521 cttcgaccgt tttttcattg caactccaat ctttggaatt ggcgcctaca cctggattcc
     2581 atgttccgtc gcaacgcttt gcgcaggtat ttttgcaggc gttttccacg agatcatgtt
     2641 taaagatcac cgcccatttg tgcttcacgc gcttgttttt atcatgctca ccgaagtatt
     2701 ccacatgggc atggtcccgc tctttcacat ggatgacatg caaaacgcaa ccatggttat
     2761 gtataacgta ttcctgccgc tgctgctctc gaacgcggcg gttgtgggtg cttcgttttt
     2821 catcaccagt tttatggaca tgcgtttcaa aggccacaag gtcagggaaa tcattttcag
     2881 caaagacatt ttcttcacac aggatttgtt cttcagtttc caattttggt tgctggttat
     2941 catttgcttc gcgtttgcaa ctaatattat tgcaatgagc ttcttcaacg accgcatgtc
     3001 ggttgccagc actgaaacgc gtttcaaaag cgacctcact tcgatgcaaa agctcatcgt
     3061 ccaagacgcc gaaactctcc aggtcagcca cgttggcatg gcgaaggttt acgcccgcga
     3121 ctggtctgac gaagaggtct cgcagtactt catcgttaac accgcgactg gcgaaatcct
     3181 cagcagcgag cgaggcgtcc cagagcttta tcaagagtgc actacaaagc ctgagctcga
     3241 cctcttcgag accaacatca acggcacaca ttattatgtt gactacgtta acttagaaga
     3301 cttcacaatt gttcaatact caattgaaaa atacgagatg tttattccgc acctgaactc
     3361 gtcatttacg atgttcctgc aaattgtcgt tttcggcgtt atgatgttca gcgttttcat
     3421 gctgctcaag aagctggttg gtgaaaacct cttaaagatc aacgaaagtc ttgagcaaat
     3481 cacaagtggt aaccttgaca ccaaggtcga tgtttacagc cacaaggaat tcgctgcgct
     3541 ctccgaccga atcaacgcaa cggtcgacac cctcaaagac tacattcaag ccgaggccga
     3601 ccgcttcaag gaagacttcg aaatggcgcg ccagattcag ctttccgcgc tgccaaacgt
     3661 tttcccgccg ttcccggaca ataaggagat tgacctccac gctaaatatc aaagtgcgaa
     3721 ggaagttggc ggtgacttct acgactacta tttcgtaaac aagaacacgc ttgcattcct
     3781 cattgccgat gtttccggca agggcatccc ggctgctatg tttatgatgc gtgcaaagac
     3841 agcgattcgt gtcttcgcag aaaagcacct tgacgcagcc aagactcttg aggccaccaa
     3901 tgactacctc aatgaaaata acgaagccga gctcttcgtc acttgctggc ttggctttat
     3961 caatactgaa actggcctca tgcaatacgc aactgccggc cacaatccac cggttgttat
     4021 caacgaggaa actggcaagt gcgaattcct cgatgcgcca atttcgctgg tccttggtgg
     4081 tatggaaggc attaagtaca ataatgctga atatcagctc caaccaggcg agaagcttct
     4141 tctctacacg gatggtatca ccgaagctca caatgaaaaa tctgagctct acggcgatga
     4201 caccgttgtc gacttcgcaa agaaaaacat caaggctgat gtcacaaccc tttgcgacaa
     4261 cctcttccag gatgttgaaa acttcgctgg cgaggctgat cagtttgatg atgaaaccgt
     4321 tcttgccttc atctataacg gcaagccaaa acctaaagtc gaaaatttgt tcgaaactga
     4381 aattagcgtt tttgcaaaga ccgaaaacgt cacccgtgtt tgtgaccgcc tcgaagagat
     4441 acttccgcaa actaacgcgc ctcttgattt cacgccgaag ttctgcgtgg ccatagatga
     4501 aatcttcagc aatattgtta agtatggata caaggatcca agcaaatatg taacggttcg
     4561 cgtccaggta gatgagcgcg atcctaagaa aaaggctgtt aaagtcacat ttgttgatgc
     4621 agctgaccgc ttcaatccgc tggatgaaag cgcgcctgac atcacactaa gcgcagaaga
     4681 gcgcgagatc ggcggccttg gaatttacat ggttcgcaag cttatggacg acgtcttcta
     4741 tatctacaaa gacaaggcaa acaacctcac cctcgtaaaa tactttggtg agggcgcacc
     4801 aagcaaagaa gaaattcaaa agatgcagga agaagaaaac aaaaaacaag aagcagaatc
     4861 tgcagcaaag aaagaaccaa aaccaaaacc aaaggaagac aaacctccaa cggacgacaa
     4921 atagcaggtt caaaaataag tagcaaaggc ccacgtaagt gggcctttta ctttaccaaa
     4981 ccagcaataa acatactttc agtaaacctt tcacaaaagc aaccccgcat ttccaattcc
     5041 ctttgttact ttcttcatta cgccaggtat aacacaaaaa agtagcaaag gggcgcccaa
     5101 acaacccact ccccgcattc ttaccagcgc aaacacaaac atcgccaaat cctcattaaa
     5161 caacattccc ctcaaccttc ctaactcatc acaccccacc gcatttccat gtgcagattg
     5221 cgtccaatcg cttaatcatg caagttaatg tatattaaag ttaagaaaat gtgtagaaag
     5281 cgttcaatcg cttattattt gcaccttatt taagaaaatc atatatttaa attactttat
     5341 tagggctttt atttttgtaa ataaataagt tagtattttt ttaagggatt gaacgaaatc
     5401 tacacatttt tcatacataa tttataattt aggttgtgtt gttaatagct ttttagctca
     5461 ttttgcaatt gtatttgctt tatagaaaaa tcttttgtgt taattcttgg gtcttgtttg
     5521 ttggttttta gtatttttat tgcaagttgg ttaaatgcat ttgggttatt cattattgcg
     5581 ggcacaaaac taaacaaatt tcaaccatca ttatgaaatg catcaagcct taatttatcg
     5641 tgcttatttt gatctgcatt ttcatgatag tatgtactgt catattctat taccagttta
     5701 gttttagggt tacataagtc tggtctaatc ttgctataac ctattatgtt agaagctgcc
     5761 ttgctaagtt gaatattttg attaaactta atgttcctta ttccgtaacc tccaagtttt
     5821 ctgggagcgc ataactgcac atataatttt gattcttgtg gtgaagcaga attgttagat
     5881 aagtactgtg caatattctt tgctttagca attccgcgaa aatgtgcttg gcatttttga
     5941 agtgaattta tatatttttt tattctttca gcagtggtaa gtgcaggtac attaggcata
     6001 aaagaattgt cttcttcatg acgcattgca tatgtgccac ataattctag tccaaataat
     6061 agaagcctaa caggcgaaaa aacgctagcc atttggcaaa ataacaattc ggggcaaaca
     6121 acatataatc cctttgctac ttttaaaaac gaatacggtg gcaattttcc acttataaga
     6181 tggtaattta cgagtttagt tttattttga tttgaagact tagaaactat aacatcaaca
     6241 gactttgttg tgtcaacata tggtatatag cttttagtta tatcattcgt tcttattgtc
     6301 aagttgttat tgtgagatac attagattct gtcagctggt atttatataa gtttggcgtg
     6361 tatatgtttg cgcctaaaat attattaccc cttattgtaa gaatttctag atagttattt
     6421 gaacgtgccc ctaaatagaa ttcaattcct gtgaaattac tgataaagat gttcattttt
     6481 ggctcctttc gtggtgcgtt taaggttcga agttcgcgcg tttttaagtt ttatcccgct
     6541 tgacatttgc cgaaatcgaa gtccggcacg ggtttgttat gatatactat tgcatagtca
     6601 tgcgcacaat tatttatgaa gatttttaaa aaaaataatg acacatcaca gcaggtaaaa
     6661 gacttcagag ctgccgaagc cgagaaacgc cgttcaatta ccaagcaaac atttattggc
     6721 aatttgcgaa cgatgtcagt ttccattttg gcaatcacgc ttgctatggt tatcgatggt
     6781 attatcatcg gccagttttt gggtaccgat gcaattgcag gttatggttt agcgtccccg
     6841 atttttatga ttattaatgc catctttgca gtttttggaa ccggcgctca atcagtttgt
     6901 gcaaacttca tgggccgagg ggacatgaaa aaggccaacg gaatttttaa tgccactcta
     6961 attgcaacgt tggctttgtc agccttaatg accctagtgt tgattattgc tcttgatccg
     7021 ttggcgcaaa tgctttgcgc tacaaatagt ggcgataacg ccgaagccct ggtccagggc
     7081 accaaagact atatcttggg aattacagtt ggtattccaa tccaattttt atgtcgtgtt
     7141 atttcaccat ttatgcagct cgattctgac cgtcaacgcg ttttaccttc aacattatca
     7201 ggctcgggaa ttaacatagc gggcgatctt ttcaacgccc tcgttctaaa ctggggcttg
     7261 ttcggtatgg cattggcaag ctctgttgga ttttgtggtg aattaacaat tttgttgttg
     7321 cattttcgta aaaaaacagc gatgtataaa atcaacttta agcttagcag ctgaaaagga
     7381 tgaggaaacg ttgtttgagt tggtcttcca actgcactta gtagagtttg tggcacgctt
     7441 cgcacacttt tcctaaatta tcaactttta ttaattggtg gaagtgttgg ggttgctgcg
     7501 ctcacggtac agcaaaactc acacaacttt tttgcttgcg taggcactgc gctgggtggc
     7561 acggtcctgc tcatggcaaa ccttgcatat ggcgatgacg acaccaccca gctcaagcaa
     7621 ctcttgcgtt tggcaataaa agccgcaatt tttgcagtcg gtgctgttgc attgctgctg
     7681 tttattgtgg ctccatgggt ctcgatgctc tattcccaag accccgaagt tttggccatg
     7741 gctacaacag caatccattt ctatgcaatt tcaatgccta tttatgcatt tgttcaaata
     7801 gttcaaaact tttgccaagc ttctaaaaaa gttacattgg ctatgataat ttgtgtgttg
     7861 gacaacttct tgcttatcgt tgtgccagcg ctaattttgc caaacttctt tggcatcaat
     7921 ggaatttgaa tgtcatttat tgcttgcgaa attttgatgg tgctggttta tacaattatt
     7981 gttatggttc gtaagaagtc gccaaaaatt aaacttgacg attacttgct tcttccaaaa
     8041 gaatatgacg agttagaatc aaacaacttt gaatttacaa tccacgacga agaagatgta
     8101 atgatgtcct cggttggagc ggaagccttt tgcattaaga acaacatcga cgaaaagcgc
     8161 acgaacgcgg ttgctctttg catcgaggaa atgggcgcaa acattgttga gaacggcatc
     8221 agggaaggcg aaaagctatt ccttgatatt aaagttattt tacgtggcga agacattgta
     8281 atccgacttc gcgataattg ctcagacttt aacccggtag accaccataa aactgacagc
     8341 gatgacccgt ttgcgggtat cggcattaag atggtattta agatggcaaa agatgtaaac
     8401 tatattatga cgatgaatct gaacaactta gtaatcacaa tttaagaagg gaattaaaat
     8461 gaaaagtaaa tttgagaagg gcacgcttac tgtcaagttc ggagtaaaaa atgcgtctgg
     8521 aagttttctc gctaaattcg ataagttcat gaacgaagaa ataaggaagc ataaaccaaa
     8581 gaaaattgtt tttgacatgg atggtgttga aaaagttact tcagcaattc ttcgccgttt
     8641 tttgcgtatt attaattttg gatttgaaga agaaattatt aatgcttcta aaagtgttgt
     8701 tgaaattttg accatcacaa atattgacaa aatgattcat gtggtacata aaagacctct
     8761 tattagtatt aaaaatttaa aggaaatttc acgtggtgga tacggcgtgg tttatgaata
     8821 ttcaaaggat gaaatcctaa aagtttataa tgggcacctg cctgaagaac aaatcttgcg
     8881 tggagtaaag aattctaagg tggcttttca acgtggcctt tctgttgcca tcccgtatgc
     8941 aactgtagat accgaagaag gcatcggtgt gctttttgaa aaagtaaaag gtacggcact
     9001 tgctgatgtt attcacaacc atccagaaag ggtggagcca aaaattaagc gtcttgttga
     9061 attgtctaaa gaaatggcag ctactgaatt taaacctggc gaactaacaa gtgttcgtga
     9121 tatttatatc aatgcgcttc actctgtcag aaagcaactc ccacctgcat atattgatgc
     9181 atataagctt gcaatagaat cccttcctga tgtcaaccgc gcggttcatg gtgatcttac
     9241 atgtgttaat gtcctggatg atggcttcga tttaatcatg gtcgacatgg acacgttttc
     9301 gttctgccat ccaatttgag acgctgcttc aatgtatact gcttattatt attgttatga
     9361 aaaaggcgac gaagaagaaa ctttcttttt taatcgtttg acatgcgctg agtgtaaaca
     9421 tgtttgagat gtttttgaaa aggaatattt taaaggtata agcaataagg aaaaaaagag
     9481 aagaattgct gcctcagcta attggggtct tttgcgaacc gctgcacttt atagtcaaaa
     9541 tattgatgag ttcgataaga ataatccacg tcatgttgag tttttgggct atatcaaagc
     9601 tattttggaa aacagcgttt tgaaaaacct atattcgatg gttgaaattt tcaacacctg
     9661 aaagatagat gagtaattgc aagtataaaa tttcagtaat cacgccgctt cacaacgaag
     9721 cggcggacat cttcgcgccc gcatacaatt cgatgctcaa gcaaaccatt ggttttaaaa
     9781 acatccaatg gataattgtg cttcataatt gcgatgtcaa aacaactaat tatgtaacgc
     9841 gcaagaccaa aaattttaag aacgtttgtt tgaaaattct aaacaacgaa gttcacaccc
     9901 caagctctcc aaaaaattac gcgatgcaat tcgcggaagc taaatacatt gcgtatttgg
     9961 atgccgatga ctggtatctg ccagacggcc taaaagttgc ctatgagcag gctgaaaagg
    10021 aaaacgcaag catcgtaagt ttcaggcggc agcatagttt tcaagaaccg gggctctacc
    10081 cgctaatgga aattacagcg tggaatcaag ccgaagataa aattgtttgc acgcagaaaa
    10141 atgtaaacac taaaaattta tacagcggtc aattttattt tttaacatct aagctttttc
    10201 gcaaatctat gcttatcaag aataagattc aatttgaaga agacatgcca cttggcgaag
    10261 attttgtttt tgcaattgaa gcttttgcta atgcaagtaa gattataatt ttaccgagat
    10321 tcattggcta ccattatttc atccgaagca attctatggc gcaaaagaat ttgtcaagcg
    10381 atgaagcctt tttatgatac gcaagaacta tcaaaaaagc ggcagatact ttcgtgaaat
    10441 ataaagtaaa tgcagactct ttacaaggta ttttactgtg aatttcgttt agaattttgt
    10501 caacgccaac tcttaaggta aaaacgcgag aagaaatttc tcgcatgctt acaaaatatt
    10561 tgaagcactt taacattcgc acgcaagata aatttcacac ttttgagcaa taccatcaac
    10621 attattgaat cccaaatgtt gttctcaaaa acccgaagga tcccgttgcg gcttaccaaa
    10681 aagaaattgt ttttgacgga actattaatt tgtgaaatat cattaagtca aattcgggga
    10741 cagattttgg gcaaaaatat aattttgcgg gaatccaaac tttggatgaa ttccacaaaa
    10801 ctgttccgcc aaccactcgc gcgcgtttgg atgaaactct aaatttgcaa acaaaaattg
    10861 gcgattccgg aattcttgtt aacggaaaac cttggcaata tttgcgaagc aattgcggtg
    10921 aactttttat gaacacaagt gctcaaaacg aagtttttac ctctgcttta gctaacattt
    10981 gcgctgataa acctacattt tttgtcaata gcggaaaaat gcaaacagac cactcaagta
    11041 acgataatgt tgagatcaaa agtcttgaat ctgttttgtg tgacaatgca tttttgttcg
    11101 cgcataaaaa taattgcgat aatttcatct ttgattttgc taaagctaag tccaagaaaa
    11161 atctgaagca aatttttgct ttggataccc aatgtttggt tgaagcattt gaaaattttg
    11221 aaaagcaaaa taaaaatctt tttaagaaag ccggccgcaa accaatcgca aaatatttat
    11281 ggccaaattt ggaagctgtt attgcaacca aaaaatcaac aaatgtaaag cttaatgcaa
    11341 agctaaaaaa gtattttggc aatgttcatt tctcaaatgc attgtatttt accgaagagg
    11401 gaattattgg aaaacgtgtt gcaggaaagc aaaatttgta tgaaattttc gatgataaaa
    11461 acgtttatga gctaaaaaat tgccaaacta acaaaatttg taatttttca aattgcaaag
    11521 aaggttcaaa ttactatctt gttattacca ataatgctgg cttatacagg tatgttagca
    11581 gtcataaaat ccaaattaaa caaaaaaata agtttaaaat cac
//
LOCUS       DBNCDC010000026        11436 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_2234_length_11436_cov_7.939548, whole genome shotgun sequence.
ACCESSION   DBNCDC010000026 DBNCDC010000000
VERSION     DBNCDC010000026.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 11436)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11436)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11436
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2234_length_11436_cov_7.939548"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(202..582)
                     /locus_tag="AC04VQ_03060"
     CDS             complement(202..582)
                     /locus_tag="AC04VQ_03060"
                     /inference="COORDINATES: protein motif:HMM:NF024231.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIO9553176.1"
                     /translation="MAVSVITTIDSQYNPYAVVYFDEFGSILASIILEPTAGFVIALL
                     NDALFAIYTGSPYAILTFGCGAMYSVIFGVYYRRGRKINAKSILLTILVLIVLNTVYI
                     VVIYALVMPSSSLNELDINYDNIV"
     gene            complement(595..1350)
                     /locus_tag="AC04VQ_03065"
     CDS             complement(595..1350)
                     /locus_tag="AC04VQ_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012730.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIO9553177.1"
                     /translation="MVTVAFISIVPLMFKSEQVSPEIDMFLNILDVATVYVLFADYVF
                     RWLTYDIKSGLGKKAFLIYPFTPMAIIDLLSILPSITILHDSFKFLRILRIVRILRMF
                     NGLTIMTNVFIRERKALASIMFLLVVYVLTVGLIMFTIEPDTFNTFLDALYWSTTALA
                     TIGYGDITPISDFGKFIAIVSSLVGIAVVAFPAGIITGGYITQLQKARSKGHEYFALP
                     IKHDKVFKGKQITKYKSISSYFKTNKKVKYYSI"
     gene            complement(1474..1719)
                     /gene="rpmA"
                     /locus_tag="AC04VQ_03070"
     CDS             complement(1474..1719)
                     /gene="rpmA"
                     /locus_tag="AC04VQ_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIO9553178.1"
                     /translation="MAHKKGLGSSRNGRDSHSKRLGCKMFGGQQVKAGNIIVRQNGTR
                     IHPGENVGRGKDDTLFAMCDGKLEFTQGAKRKVHVRK"
     gene            complement(1735..2319)
                     /gene="rplU"
                     /locus_tag="AC04VQ_03075"
     CDS             complement(1735..2319)
                     /gene="rplU"
                     /locus_tag="AC04VQ_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIO9553179.1"
                     /translation="MKATIKTGGKQYNVGLGDVISIEKIDAKAGDKVALTAICIIDGD
                     KVEADPAKAAKTVVKAEVLEQFKGEKQIVFKFKRRKNYKVKRGHRQQLTKVRIVEIGS
                     AKFDPKKVAKPAAKPAAKKEAPKKAEAKPAAKPEVKAEAKKPASKPAAKPAAKKTDTK
                     KPAAKPAEKKAPAKKPAAKPAAKKAPAKKPADKK"
     gene            2745..3002
                     /locus_tag="AC04VQ_03080"
     CDS             2745..3002
                     /locus_tag="AC04VQ_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIO9553180.1"
                     /translation="MEQALKIAKRLGYKTKNHRNMIGIADLEGESKTQIGIIGHCDVV
                     PPGTGWHFDPYKLNIVNDYMIGRGVLDDKGPTVIFLHAINF"
     gene            3045..3287
                     /locus_tag="AC04VQ_03085"
     CDS             3045..3287
                     /locus_tag="AC04VQ_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553181.1"
                     /translation="MGTDEETFSTDVNEYLKHYEEPRFVLTPDADFPVPYGEKGIVRF
                     TVSQKIKNGEIISIKAGQTNNAVAGKAVAEIDGSKK"
     gene            3420..3872
                     /locus_tag="AC04VQ_03090"
     CDS             3420..3872
                     /locus_tag="AC04VQ_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553182.1"
                     /translation="MLSQFCIEKNIGNAEELAFMKFLSKIAGKPRGEGVGMNWKDEHF
                     KDLTIVPTILQCQNGQISQDFDVRCPNSVTPDKVKKQIEKHMPKGSVYEKISNFPAYL
                     VNPKSKLIGALQKSYEQATGEEPRLFTMGGGTYAKCFKCGVSFGPLRD"
     gene            4082..4342
                     /locus_tag="AC04VQ_03095"
     CDS             4082..4342
                     /locus_tag="AC04VQ_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553183.1"
                     /translation="MNDANKYRDLSRYRNQTPTQNDIPLGSNKDFQASANLDYKTAQY
                     SRDSGAKYAHKHVKRNDRARRRKNIIRTAAVIVVLAVVGIGV"
     gene            4547..4696
                     /locus_tag="AC04VQ_03100"
     CDS             4547..4696
                     /locus_tag="AC04VQ_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553184.1"
                     /translation="MHDGDELTYTDGKDLMEDYTSDDSDVAAKAQIVGAGAVHKFEGT
                     GEPGT"
     gene            4739..5446
                     /locus_tag="AC04VQ_03105"
     CDS             4739..5446
                     /locus_tag="AC04VQ_03105"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIO9553185.1"
                     /translation="MTKDPDDVTVRQVNIDPGKDKVIALTFDDGPSDEYTQDILDLLK
                     KYDAHATFFVVGERLEENWGKKLVAKEHSAGHQVCTHSYDHARAAGGTDMTGMSAEKQ
                     IQEITKGKQMISKAIDGDVSSVVRVPGGNLTANTCRLIAPFVSAEIGWNIDTGDWEMP
                     GTEKILKNLKLASTGDIILCHDGGGDRHETVEALNDFLKEYTSKGYKFITIDEMMQYD
                     EAKQKDDTTASTAKYAS"
     gene            complement(5547..5819)
                     /locus_tag="AC04VQ_03110"
     CDS             complement(5547..5819)
                     /locus_tag="AC04VQ_03110"
                     /inference="COORDINATES: protein motif:HMM:NF040140.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase family protein"
                     /protein_id="HIO9553186.1"
                     /translation="MQNLQITNIVVKKFRIICTVKLNNVKYSNEAIKTTLLEKMPTLA
                     AHKCKNSKGKNFVEVMDSTSLAHVLEHMIIDIQTKHTDKALFGTTE"
     gene            complement(5820..5972)
                     /locus_tag="AC04VQ_03115"
     CDS             complement(5820..5972)
                     /locus_tag="AC04VQ_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553187.1"
                     /translation="MSQIDLSPAYTKNNKTDVNTDNTGIEDIISNGQTQSILLDRIST
                     KKPDEA"
     gene            complement(6276..6578)
                     /locus_tag="AC04VQ_03120"
     CDS             complement(6276..6578)
                     /locus_tag="AC04VQ_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553188.1"
                     /translation="MADEQQNNQSSVDTNKIPKVTQEMLDTFADPKPIEEEQETTEQV
                     AEISEKPSEDKTVVEFSEDKTEEEQKPAKPKKFGFFLKALVVILLIAFVGTAIFGI"
     gene            complement(6579..7172)
                     /gene="serS"
                     /locus_tag="AC04VQ_03125"
                     /pseudo
     CDS             complement(6579..7172)
                     /gene="serS"
                     /locus_tag="AC04VQ_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012796.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
     gene            complement(7263..7844)
                     /locus_tag="AC04VQ_03130"
     CDS             complement(7263..7844)
                     /locus_tag="AC04VQ_03130"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF014458.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0016260 - L-selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIO9553189.1"
                     /translation="MLDIKYVRENTDSVVAAMKNRNADFDKKKFAKFEEDRRKCIAVE
                     EELQAERNSASKKIGELMSQGKKDEAEKAKQDVRKLNDKLEKATEARGRADRALREFM
                     LAVPNIPHETTPVGKDENDNPEVRKWGEPRNFDFEAKAHWDIGTDLDIIDFERGVKLS
                     KSRFYVLGGAGAKLERSLINYMADTHEAKGYKE"
     gene            complement(7887..8117)
                     /locus_tag="AC04VQ_03135"
     CDS             complement(7887..8117)
                     /locus_tag="AC04VQ_03135"
                     /inference="COORDINATES: protein motif:HMM:NF019952.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553190.1"
                     /translation="MDFERLMGHSNLDICFGGEAFDELAECFRTKKITANRVQNVKDL
                     LEQSSKSKNIYIIANYTAMFPLHDELTKLASE"
     gene            complement(8127..9221)
                     /locus_tag="AC04VQ_03140"
     CDS             complement(8127..9221)
                     /locus_tag="AC04VQ_03140"
                     /inference="COORDINATES: protein motif:HMM:NF019850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIO9553191.1"
                     /translation="MSAKSDIAKTTAKFSRFVLQKILRRPGGFFPGDLALKIDPELLV
                     ELSSQFDAGTVLVTGTNGKTSVTNLIADCMRYEGAEVAWNKTGANLASGVASAIMEFS
                     PKRRKKKFGVFEVDELWVEKVLPQTKSKFLLLLNLFPDQVDRFGNIGNIQKSLITALN
                     KSRETVLVYNADDPNCQVVADECDNNTIPFGIGEKISKIDDGAETKCPLCEFKLNYQL
                     HQYAQLGDYRCANCGFNRAKPKYLTSNIQLGKDTLQFSVNGKTYVSQKAAEYACYNLT
                     GFVALADALGCHNDSIDKSIKYQQAKNGRMEFFNVTGREVMINLAKNPVGFNQNISYV
                     LDRYNSDEQPVHTTVAFFVNAREGDGKNPN"
     gene            complement(9242..9329)
                     /locus_tag="AC04VQ_03145"
     tRNA            complement(9242..9329)
                     /locus_tag="AC04VQ_03145"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9293..9295),aa:Leu,seq:cag)
     gene            complement(9352..10734)
                     /locus_tag="AC04VQ_03150"
                     /pseudo
     CDS             complement(9352..10734)
                     /locus_tag="AC04VQ_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            10967..11041
                     /locus_tag="AC04VQ_03155"
     tRNA            10967..11041
                     /locus_tag="AC04VQ_03155"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11000..11002,aa:Glu,seq:ttc)
     gene            11089..11164
                     /locus_tag="AC04VQ_03160"
     tRNA            11089..11164
                     /locus_tag="AC04VQ_03160"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11122..11124,aa:Lys,seq:ttt)
     gene            11175..11248
                     /locus_tag="AC04VQ_03165"
     tRNA            11175..11248
                     /locus_tag="AC04VQ_03165"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11207..11209,aa:Gln,seq:ttg)
BASE COUNT         3673 a         2382 c         2029 g         3352 t
ORIGIN      
        1 ctcattttta ccacgcttat aaatcttttg tttatagcgt ttacgtacag gaattttcat
       61 catcgggtca agtctgcttc cccagaagaa ctggcgaatc aaaacaacaa ttacgaaaca
      121 taaaattgca taaacagttc ttccaaagaa ataatagaaa actattcctg aatatatatc
      181 aagtcctcaa gtcgaagtaa atcaaacaat attgtcataa ttgatatcca actcatttaa
      241 agaggagctt ggcataacca aagcataaat tactacaata taaactgtat ttagaacaat
      301 taagactaat atagttagaa gaattgactt agcattaatc tttctaccac gtctataata
      361 cacaccaaaa attacgctat acatagcgcc acatccaaat gtaagaatag cgtaagggga
      421 acccgtataa atagcaaata acgcatcgtt caaaagtgct ataacaaaac ctgctgttgg
      481 ctctaaaatt atcgaggcca aaattgaacc aaattcatca aaataaacta ctgcatatgg
      541 attatattgt gaatctatag ttgtgattac agagacagcc aaacaaatta aaattcaaat
      601 tgaataatat ttaacttttt tgttagtttt aaagtaggag ctaatgctct tgtacttcgt
      661 aatttgcttt cccttaaaga ctttgtcgtg tttaataggc aaagcgaagt attcgtgccc
      721 ctttgaacga gccttttgga gctgtgtaat gtaaccacca gtaataatac cagctggaaa
      781 agctacaaca gcaataccaa caagggatga cactattgct atgaattttc cgaaatcaga
      841 aattggagta atatccccat aaccaattgt tgcaagagca gtagttgacc aataaagggc
      901 atctaaaaat gtgttgaaag tgtcaggctc aatcgtaaac ataatcaagc caacagttaa
      961 aacgtaaaca accaacaaaa acattatcga agctaaggcc ttacgttcac gaataaaaac
     1021 gttggtcata atagtcaaac cattaaacat gcgcaagatt ctaacgatac gcaaaatacg
     1081 caggaactta aaactgtcat gcaaaattgt aattgatggc aaaattgaaa gaaggtcaat
     1141 aatggccatc ggcgtaaatg ggtagattaa aaatgccttt ttacccagac cacttttaat
     1201 gtcgtatgta agccatcgaa atacataatc agcaaagagc acataaactg ttgcaacatc
     1261 taaaatgttt aaaaacatat caatttcagg tgatacctgc tcgcttttaa acatcaaagg
     1321 gacaatagaa ataaaagcca ctgttacaat gaaaaaatcg tatcagcggc taacttgatc
     1381 gttgttttga gctctttgaa ttatttgaaa aaggcgaagc ctcatatttc cttcttatga
     1441 aacgctacct tttatgatgc aatcagtccg ttcttattta cgaacgtgaa cttttctctt
     1501 agcaccttgt gtgaactcaa gtttaccatc acacattgca aaaagagtat catccttgcc
     1561 tcgaccaaca ttttcacctg gatgaatacg ggtaccattt tggcgaacga tgatatttcc
     1621 agccttaact tgctggccac caaacatttt gcaaccaagg cgtttgctgt gcgaatcgcg
     1681 accgtttcta ctggaaccta aacctttctt atgagccata atgcacctcc taaattattt
     1741 cttatctgca ggcttttttg ctggagcctt ttttgcagca ggttttgctg caggcttttt
     1801 agcaggagcc ttcttctcag ccggtttagc ggctggtttc tttgtatcag ttttctttgc
     1861 agcaggttta gcagctggct tcgaagcagg ttttttagct tcagctttaa cttctggttt
     1921 agctgctggc tttgcttcgg ccttcttagg agcttccttc tttgctgctg gtttagcggc
     1981 aggtttagca acttttttag gatcgaactt tgcactacca atttcaacaa tacgaacctt
     2041 tgttaattgc tgtctatgac cgcgttttac cttatagttt ttgcgacgct taaacttaaa
     2101 aacaatttgc ttttcgcctt tgaactgctc aaggacttca gccttaacaa cagttttagc
     2161 agctttagca ggatctgctt caaccttatc gccatcaata atgcaaatag cggttaatgc
     2221 aaccttatcg cctgctttag cgtcaatctt ttcgatgctt ataacatcac caagacctac
     2281 attgtactgt tttccaccag ttttaattgt cgctttcata atttctcctt ttctcttaaa
     2341 atcgcaggtg agcgtataaa tcagcctgcc gaaattaagg cgtaatcgcg tattttacca
     2401 gtatggtaag tgcaagtcaa gaactttttt ccccggggcc agcactcgat ttccccgggg
     2461 ccagcactcg atttactcgt ggcatttact caatttttaa tatttatata aaatcgagtt
     2521 ctggccccgg ggaaatcgag tgctggcccc ggggaaaatg ttgtataatg cgcgattgtg
     2581 caattatggc agataaaaag ttaaatatag aaattgaaaa atacttaaaa gataattgag
     2641 accaaattct tgaggacatc tcttcgctaa taagtattcc aagcagccca aatgacggaa
     2701 agttcacaaa agaataccct acaggaacag gtgctgcaaa agcgatggaa caagccttaa
     2761 aaattgcaaa acgtttaggc tataaaacta aaaatcacag aaacatgatt ggaattgctg
     2821 atttagaggg agaaagcaaa acacaaattg gcattatcgg acattgcgac gttgttcctc
     2881 cagggacagg ctggcacttc gatccttaca aattaaacat agtcaacgat tacatgattg
     2941 gtcgtggtgt tttagacgat aaaggcccaa ctgtaatttt tctgcacgca ataaattttt
     3001 gacttcaaaa atcaaaaaaa cttccatata gtattcgctt tttattggga actgacgaag
     3061 agaccttcag caccgacgtt aatgagtatc taaaacacta cgaagaacct cgttttgttt
     3121 taactccaga tgctgacttt ccagttccat atggcgaaaa aggaattgtg cgttttaccg
     3181 taagccaaaa aattaaaaat ggcgaaatta tctctattaa agctggacag acaaataatg
     3241 ctgtagctgg aaaagcagtt gctgaaattg acggatcaaa gaaatgaatc gacactgaaa
     3301 attttagatt agcacacgct ataaaaatta atagactaga aaatggcaac cttgaaatta
     3361 ttgcgaaagg ccgagcaagt catgcagctg aaccttttag cggaattgac agcatagcag
     3421 tgctttcgca gttctgcatt gagaaaaata ttggaaatgc cgaggaactt gccttcatga
     3481 aatttttgtc aaaaattgct ggaaagccta gaggcgaagg cgttggcatg aattggaaag
     3541 atgagcactt taaggatctg acaattgtgc caacaatact gcaatgccaa aatggtcaaa
     3601 tttctcaaga ttttgatgtt cgctgtccaa attcggtaac acctgataaa gtaaaaaaac
     3661 aaattgagaa acatatgcca aaaggctcgg tgtatgagaa aatcagcaat tttcctgcat
     3721 atttggttaa tccgaagtct aaattaattg gtgcacttca aaaatcatat gagcaagcga
     3781 ccggcgaaga acctcgccta ttcactatgg gtggcggaac ctatgcgaaa tgtttcaagt
     3841 gcggtgttag cttcggacct cttcgtgatt gaatcccaaa acctgcttgg gtaggaactc
     3901 tgcatggccc tgatgaagga attagcatcg aagaaatgca aacttcattt aatatttacg
     3961 ttcatgctat taagaactta atggaacttg atttggatga cgaaaacttt gttgaggaca
     4021 ctacaaaaat ttcgtagccc aatattttgt aaaaaaacac taaaatggta gaatatatcc
     4081 cgtgaatgat gcgaacaaat atagagattt aagcagatat cgaaatcaaa cacctacgca
     4141 aaacgatatt ccgcttggtt ctaataaaga ttttcaagcc agtgcaaact tggattataa
     4201 gactgcgcaa tattcgcgcg attctggtgc taagtatgct cataagcatg taaagcgaaa
     4261 tgatagagca cgcaggcgaa agaatattat tagaactgca gctgttattg ttgttttagc
     4321 cgtagttgga attggtgttt gaaaattttg gccaatacac gtccaagtaa atggtcatga
     4381 atatacacta acttatgata aaagtgttgg cgaagcagca aaacaagctg acgttaaagt
     4441 taagccagga aatttcattg ccgttgatta cagcattatt acacctgacg aaggcaatga
     4501 atattatgcc gaagttaatg gcgaagaagt aaaaaacaag catgaattgc acgatggaga
     4561 cgaacttact tatacagatg gcaaagactt aatggaagat tacacatctg acgatagcga
     4621 tgttgctgca aaagctcaaa ttgttggagc tggtgcggtt cataaattcg aaggcacagg
     4681 cgagcccgga acttgatcaa ttttacacgg taaggtttct ggtaaatctg ctgagagaat
     4741 gactaaagat ccggacgatg taacagttcg tcaagttaac atcgatcctg gcaaagataa
     4801 ggtaattgcg ctcacttttg atgacggacc ttcggatgaa tacactcaag acatacttga
     4861 ccttcttaaa aaatacgatg ctcatgcaac attttttgtt gttggcgaac gcttagaaga
     4921 aaattggggt aaaaaattag ttgctaaaga acattctgct ggtcatcaag tttgcactca
     4981 ttcatatgat catgcgcgag ctgcaggagg caccgatatg acaggaatga gcgcagaaaa
     5041 acaaattcaa gaaatcacca aaggcaagca aatgatttct aaagcaatcg atggcgatgt
     5101 aagttccgtt gttagagttc caggaggaaa tttaacagct aacacatgca ggttgattgc
     5161 gccatttgta agtgccgaaa ttggctggaa catcgatacc ggcgattggg aaatgccagg
     5221 aactgaaaaa attcttaaaa acttaaagct tgcttcgact ggagatataa ttttgtgcca
     5281 tgatggcggc ggtgacagac atgaaactgt tgaagcgcta aatgatttct taaaagaata
     5341 cacatctaaa ggttacaaat ttataactat cgatgaaatg atgcaatatg acgaagcaaa
     5401 gcaaaaagac gatacaacag cttccacagc taaatacgca tcgtaatttc attcaataat
     5461 ttcgaagctt cttttatagc agacaaacaa actatatcat ctgtgtaagt cagttcaatt
     5521 tttgcaattc cagattgctc atcaattcat tcagttgttc caaaaagcgc cttgtctgta
     5581 tgcttcgttt gaatgtcaat tatcatatgc tctaaaacat gagcaagcga agttgagtcc
     5641 atcacttcaa caaaattctt gcctttagaa tttttgcact tgtgagctgc caaggtaggc
     5701 atcttctcaa gcagcgtcgt tttgattgct tcgttagaat acttaacatt attaagttta
     5761 accgtgcata taattcgaaa tttttttaca acaatatttg ttatttgaag gttttgcatt
     5821 taagcttcgt caggtttttt agtagaaatt cgatccagca aaatactttg agtttgaccg
     5881 tttgaaataa tatcttcaat tccggtattg tctgtgttta catccgtctt attattctta
     5941 gtgtatgcag gcgaaagatc aatttgagac atgccacaag ttgatcaatc tcaaaaccat
     6001 ccaatatcac taaacattgt agaaattgga tcacaggcta cgttttcgca aagcgcaagc
     6061 tgatttctat ctcaagagaa acgataatgc gattgccccg acatatcgcc tattgtgtaa
     6121 ttaataacct tagcccctgt atcaacggta tatttcagca ccgcatcttt acttaaaata
     6181 atattttctt tgtctattac aacaacggca ttagaaccat taatttgcca gttgcttatg
     6241 agttgagctt cgtcatcgta acgaaatcat ctatttcaaa taccaaaaat cgctgttcca
     6301 acgaacgcta tcaataaaat aacgaccaaa gcctttaaaa aaaaaccaaa cttctttggc
     6361 ttcgcaggtt tttgctcttc ttcggttttg tcctcactaa attcaactac agttttatct
     6421 tcgcttggtt tttcagaaat ttcagcaact tgttcagttg tttcctgctc ctcttctata
     6481 ggcttaggat ccgcaaaagt atccaacatt tcttgagtaa cttttggaat cttattagta
     6541 tctactgaac tttgattatt ttgttgctca tcagccattt atttaatgat ttcacaaccc
     6601 atgtactttt gaagaacctt aggcacaaca acgctgccat cggcttgctg ataattttcc
     6661 aaaattgcag ccattgttcg accaacagcc aaaccacttc cattaagtgt atgaacaaat
     6721 tggttcttac catcttcgtc tttataacga atgttggctc ggcgcgcttg aaaatctcaa
     6781 caatcactgc aagatgaaat ttccttataa tcattatagc ttggcaacca aacttccaaa
     6841 tcataagtct tcgaagacga aaacccaata tccccggtgc acaaacttac aactctataa
     6901 ggaagttcaa gctgttgcaa aatatattca gcatccttaa ccatatcttc aagcgcttgc
     6961 tgactatcct ctggcttagt gaaataaacc atttcaactt tactgaattg atgaacgcga
     7021 atgattcctc gagtatcacg accagcagcg ccggcttcgc ttcgaaaaca tggagtaaaa
     7081 gcacaatatt ttttagggag atcacttgca tttaaaattt cgtcccgatg caaatttgtc
     7141 aattgaacct ctgccgttgg tattaaatac atgccatcag ttgttttaaa caaatcttct
     7201 tcaaactttg gaagctgacc cgttccagtt agagtgtctg catttgcaat ggcaggcaac
     7261 catcattcct tgtagccttt tgcctcatgc gtatccgcca tgtaatttat aagcgaacgc
     7321 tcaagcttag caccagctcc acccaaaaca taaaagcggc ttttcgaaag tttaacacca
     7381 cgttcaaaat caattatatc aaggtccgtt ccaatatccc agtgggcctt tgcttcgaaa
     7441 tcaaaattac gaggctcacc ccacttgcga acctcaggat tatcattttc atcttttcca
     7501 actggagttg tctcatgagg aatattcgga acagccaaca taaactctct tagcgcccta
     7561 tcagctcttc cgcgagcttc cgttgctttt tcaagcttat cgttaagttt gcgaacgtcc
     7621 tgtttggctt tttcagcctc gtcctttttg ccttgagaca tcagctcgcc tatttttttc
     7681 gaagctgagt tgcgctcagc ttgaagttct tcctcaacag caatacactt acgcctatcc
     7741 tcttcaaact tcgcaaactt tttcttgtca aaatcagcgt tgcggttttt catcgccgcg
     7801 actaccgaat ctgtattttc acgaacatat ttaatgtcta acatattaac tccaattgtt
     7861 tagcctggta ttatactaca caaccattat tctgaagcca gtttcgtcag ttcatcatga
     7921 agaggaaaca ttgccgtgta atttgcaatt atataaatat ttttagattt cgaagattgt
     7981 tccaacaaat ctttaacgtt ttgaacgcgg ttagcagtaa tttttttagt tctaaaacat
     8041 tccgcaagct catcgaaggc ttcgccacca aaacaaatat ctaaattaga atgccccatc
     8101 aaacgctcaa agtccacatc gtatactcaa tttggatttt ttccatcacc ttcgcgagca
     8161 ttaacaaaaa acgcaactgt tgtatgaact ggctgttcgt ctgaattata acgatcaaga
     8221 acataactaa tattttgatt gaagccaaca gggttcttcg caaggttgat cataacttcg
     8281 cggccagtga cattgaaaaa ttccatgcga ccatttttag cttgttgata cttaatggat
     8341 ttatctatgg aatcattgtg acaaccaaga gcatcggcca gcgctacaaa accagtcaaa
     8401 ttataacaag catattcagc agccttctga cttacgtaag tcttaccgtt aacactaaat
     8461 tgcaaagtgt ctttacccaa ttgaatattg ctcgttaaat atttaggctt tgctctatta
     8521 aaaccgcagt tagcacacct ataatctcca agttgagcat attgatgaag ttgataattt
     8581 aacttaaatt cacaaagagg acacttcgtc tcagcaccat catcaatctt tgaaattttc
     8641 tcgccgatac caaaaggaat tgtattgttg tcgcattcat cagcaactac ttgacaattt
     8701 gggtcgtcgg cattgtaaac caaaacagtt tcacgagatt tattcaaagc cgtaatgagt
     8761 gacttttgaa tatttccaat atttccgaag cgatcaacct ggtctggaaa caaattcaaa
     8821 agaagcaaaa acttcgactt cgtttgaggc aaaacttttt ctacccatag ctcatcaact
     8881 tcaaaaacgc cgaatttctt ttttcgacgc tttggagaaa attccataat tgccgaagcc
     8941 acgccacttg ctaagtttgc tcctgtctta ttccaagcaa cctcagcacc ttcgtatcgc
     9001 atacaatctg caattaaatt tgtaacgctc gtcttaccat ttgtgcctgt aaccagcaca
     9061 gttccggcgt caaattgaga actaagctca actaaaagtt caggatcaat ctttaatgct
     9121 aaatctcctg ggaaaaagcc cccaggacga cgaagaattt tttgtaaaac aaaccgagaa
     9181 aacttcgcag ttgtttttgc tatgtcgctt ttcgcgctca ttgtcttaat tttaccataa
     9241 ttggtgcggg cgaaaggatt tgaaccttca cagagctagg ctccatatgg acctgaacca
     9301 tacgcgtctg ccaattccgc cacgcccgcg caggcgcaaa ttataactca attatttaat
     9361 agtcgggaca ggagttttat ctatgtcatc aacgtgagaa ccacgaacat agtgacgcgt
     9421 ttccttcgcc actattccag taaatgccaa aaccacaata atatttggaa ttgccataaa
     9481 agcgttcata atatctgaaa catttcacaa aatatcgcca acacccaagg ctccaagaaa
     9541 accagttgcc aaataaatta catgataaac tcgaatacct tttccgccag ttaaataggt
     9601 aatgcatcta tttccgtaat attgtcatcc aataattgtt gaataagcaa ataaaattat
     9661 tccaaaaatc agcactcaat ttccaaaagg tatttgacca aatgaagcaa ttacaagaga
     9721 ggtgccatca atagtttcac ttgacgctat tcctaaaagt ccagacaaat tatcttgatt
     9781 agcaatcatt gttgtaacaa ttacaaaacc agtcaataaa caaattacaa cggttcctca
     9841 aaaagttgca gtcatagcaa caagtgattg cctataagga ttttttgttg atgccgccgc
     9901 agatatcatc ggcgaagacc ccataccact ttcatttgag aacaaacctc gagcacatcc
     9961 aaattgcaaa gttgctgcaa ttccacttcc gatgaaacct ccgaaggcac ttcgtcccgt
    10021 aaatgctgtc tccataatca aacaaaaagc aggtcaaata tatgcccaat tcaaacatat
    10081 gattaaaata gaaccaccaa cataaagtaa acaaatcagt ggaactaaag cttcgcaaac
    10141 cttagaaata attttaatgc caccaaaaat tacaacagcc gcagcaatag ttatgattat
    10201 tgcaaatacc caatttggca cgtcaacaaa aaccgtagac agcgaattgc taatagccga
    10261 agtttgaacc gcgcaaccaa ttccaaaaga tgcaagaatt gtaaaaattg cgaaggcccc
    10321 ggcgccaagc ttcgtcgctc aatgattagt taaaacttct ttcttgtttt tattaaaagc
    10381 acgacgaaat atatacatcg cgccgccaag catttctcca ttagcatctt taactctata
    10441 tttaagagct aacattgtct cagcatattt ggttgccata ccaaaaattc cagtaagcca
    10501 cattcaaaaa attgctcctg gaccacccgc tgcaatagca gttcccacac caataatatt
    10561 accagtacct aaagttgctg aaagtgccgt gcaaagcgca ccaaactgac ttatatctcc
    10621 ttcggaatca ggatcaactg taactgaaag ttttatagca gtaggaagtt ttctttgaat
    10681 aaatccagtc ctaaatgttg tgtataaatg cgtgccaagc aaaatggcta tcatgggaac
    10741 gcctcaaaca aaagcatcta cactattaat tattccagta aaaacttcca taatctcccc
    10801 ttaacttttc cgcataatac tacaagtgaa ctaatgcaaa accactatat aaaatttttt
    10861 gaaattatgc ttgcaaagtg cgagtagttt tgcgataata ccccacgctg caaaatttga
    10921 ctttgccccc ttataagagt cagattttgc agcagctgtt ttaactgtcc ccttagtcta
    10981 acggttagga cacgggccct tcaagcctga gatacgggtt cgattcccgt agggggcacc
    11041 agttagcaat aaaactgcag gtaaattatt acttgcggtt tttttgacgg accggtagct
    11101 cagttggtag agcagcggac ttttaatccg aaggtcgggg gttcgagacc cccccggtcc
    11161 accatcttta aagttgggga ttcgtccaac ggtaggacac cagtttttgg tactggctat
    11221 gcgggttcga ttcctgcatc cccagccact ttaattctta atttctaatt atttagtttt
    11281 gtttgaatta cgttgcgcaa gttcatccat aatctcagca tgacgctttt cggtaagctt
    11341 atagaaaaac atgggcaatg ccgccaaagc cattatgcct gcgttaatta atgtaaacaa
    11401 aagatgaaaa ccatctaata cggcaggtgc ttgaac
//
LOCUS       DBNCDC010000027        11017 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_2397_length_11017_cov_7.560299, whole genome shotgun sequence.
ACCESSION   DBNCDC010000027 DBNCDC010000000
VERSION     DBNCDC010000027.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 11017)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11017)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11017
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2397_length_11017_cov_7.560299"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            245..676
                     /locus_tag="AC04VQ_03170"
     CDS             245..676
                     /locus_tag="AC04VQ_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553192.1"
                     /translation="MLTSAALNNGQPTTNVGDYYYPGTSEQNKASMTIKGNMSSLLSN
                     YTFTITTHLKITTNDISSMYELDEFTSSYTYNGYDQFPTDIKVVKKGDHTVELPKTEY
                     TIVGNKYKNVGENYSFHIEAKEGSNLTGSIYASTSPDTDFT"
     gene            1061..1183
                     /locus_tag="AC04VQ_03175"
     CDS             1061..1183
                     /locus_tag="AC04VQ_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553193.1"
                     /translation="MSLTDDYVVTSSKQTDVPESGVYTFTIQGVGNYVDSISKE"
     gene            1538..2659
                     /locus_tag="AC04VQ_03180"
     CDS             1538..2659
                     /locus_tag="AC04VQ_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553194.1"
                     /translation="MNLTIEKSDGSIVLTPLDYDVIGSISEKDVPDPKVSDHYEFHIK
                     GKGNFSGEIWNGSPNVPFTWKVKPASVTYNYVDPQSPLTYNAEKYENDVVIGEGTLFS
                     TDEIGLNVVSDSKNVGTYSFPTTNADGNLIPTLSEINTPLRNYDLTLTGSIVINKKQI
                     NPEDIIPVCPEYNGDEQSPVIVIKDKDTGEALISGDDYTLSGITEATDVAATDMIINA
                     TLDPGANYSGIIPAIPWNIRPKAITIKITEEKVFDGKTIIVEGTLPTGVYEQSFDYSY
                     ETEDSAVGVYSREKGNLIGGYEPTDEETKVTNYAVNEDVVLTINGVPPEPSPDVPEYV
                     PGQTGDSTPVAGFALMALTLIGAFLFLRRPKKVQGLHIR"
     gene            2935..3240
                     /locus_tag="AC04VQ_03185"
     CDS             2935..3240
                     /locus_tag="AC04VQ_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553195.1"
                     /translation="MPADSTKPRRLNASDSPKNYLLERLLEEKRSKISGGIYHKTQID
                     LTYNSNHIEGSRLTHDQTRYIFETNTIGIENDDINVDDVVETTNHFRCIDFIIDKAN"
     gene            3337..3711
                     /locus_tag="AC04VQ_03190"
                     /pseudo
     CDS             3337..3711
                     /locus_tag="AC04VQ_03190"
                     /inference="COORDINATES: protein motif:HMM:NF014691.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            3868..4368
                     /locus_tag="AC04VQ_03195"
     CDS             3868..4368
                     /locus_tag="AC04VQ_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553196.1"
                     /translation="MSVETLIRFVAVTPGGGGGDDPIINPGSWNAQTGDMFGMIIAIL
                     LAIACIAAFALRAYSKSRVYAGSEAKASASTSFKGLRTAMAVIIAALACAMLVNVTSM
                     QVAAKQQTANGEITSPASIEAHVDETAGTITIDNSYFMNGSDTDSVLNSLTVSSAMEV
                     NDGGAT"
     gene            4381..5274
                     /locus_tag="AC04VQ_03200"
     CDS             4381..5274
                     /locus_tag="AC04VQ_03200"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIO9553197.1"
                     /translation="MNSTLYNDVIGKTVTFTDTKTIAAGTKEDIKISTNMDIATAKGL
                     IGQNVLKMSWTYSDSPTPPPPPPPPGEQIEIKGVAVDADGLPIANATVTIYDEAGNAI
                     ATATTDASGNYTLTLPEDMSKLDKGVIKVKTSGGVSQDIIVENITQDTTLTEKSTVEP
                     STTISGTITDEDGNPVAGVEVTYTTVVTYPDGTTKVETFTGITDPNGKYTIDVKQDHN
                     YIENVDKKTEPQIHTSDKIEVEKTPISNDIEVTETLYTVTVNLGNGSVLYVPEGWTSS
                     TAGVYTKDLNKNSDIPSIIAM"
     gene            5320..6060
                     /locus_tag="AC04VQ_03205"
     CDS             5320..6060
                     /locus_tag="AC04VQ_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553198.1"
                     /translation="MTSDSSTDALLKPTLIKASYDAHAYINGKALDEDGQPLYPGKAV
                     FIDENGNTTAVTLNEDGTYSLPIDPNTSGTFHIESTDGEYSPDYKISGDEGVTNIPET
                     YVEPSTKISGTVYGPDGKPLKNTEIEITTYVERDGKTVEEKYIVKTDENGHYEKNVQQ
                     NKDYTIKVPIPDTNPPVNYEEDIEVLTTEVEVPKIDVPSATETYVATINLNKGTIPRA
                     LSGWQKTTAGTYTKTFAKNSTYEAITSS"
     gene            6421..6885
                     /locus_tag="AC04VQ_03210"
     CDS             6421..6885
                     /locus_tag="AC04VQ_03210"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIO9553199.1"
                     /translation="MVEADKSVVETSHKTTGKVTDATGDPIAGADVTFQTTTYVDGEK
                     VIETYTTKTNSSGEYTIEIKENKKYDVEIKKESNPKIDFIQSVEVVPGDTSPINVDIP
                     KGQMATFSVDLGEGVILPEDVPAGWTKSTEGVNTYEKQMLQSKQTMEAVIKL"
     gene            6919..7278
                     /locus_tag="AC04VQ_03215"
     CDS             6919..7278
                     /locus_tag="AC04VQ_03215"
                     /inference="COORDINATES: protein motif:HMM:NF018867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIO9553200.1"
                     /translation="MLSEIIFETDTHYKAKYSPLKTISGHILDEDGSPVPGSVVKLYD
                     SEGNVVDETTTDNNGQYEFNVAQGNEYKIKANTPTGTTTEYDVGKVEEDMNLPNIEIF
                     PKAKLHGQITDSEGVGM"
     gene            7294..7575
                     /locus_tag="AC04VQ_03220"
     CDS             7294..7575
                     /locus_tag="AC04VQ_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553201.1"
                     /translation="MSYKLEDGTKIQSSAVTNKNGFYSVELPSGFNYDIEILGYSYGT
                     YVGELRVDQVDQTFNYQFGIYKTAGVLLYNVFSSGGTEHPDGWSVSSSG"
     gene            7780..7938
                     /locus_tag="AC04VQ_03225"
     CDS             7780..7938
                     /locus_tag="AC04VQ_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553202.1"
                     /translation="MTLNPATFDGLVPVGNSSYEVLEDNSIKIVTEATVLVVAAPVPA
                     SDSYTFDG"
     gene            7972..8187
                     /locus_tag="AC04VQ_03230"
     CDS             7972..8187
                     /locus_tag="AC04VQ_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553203.1"
                     /translation="MIQGKMDFVANFRLLGNMFPKAVFKASDSSFTFYYDDIDHSIDA
                     ADGDVQYILEKDTHLDFNDLSTTVYAP"
     gene            8341..9186
                     /locus_tag="AC04VQ_03235"
     CDS             8341..9186
                     /locus_tag="AC04VQ_03235"
                     /inference="COORDINATES: protein motif:HMM:NF015347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HIO9553204.1"
                     /translation="MIKAQSYSGFENIADCVESCNTTFCNFGGIGDELNEMDVHNASL
                     DLTNFHQSKLANVQSMFSTCLFNELIIPDKFAPKATSFGGVVTLGPDQAIVTGMFSAA
                     LCNKIIINGTIGTGVTDFTGLFTQCTNIKTQEDASIDKVDYSSAVDVTQAFGGLSFDK
                     FVLPTSFKNATKLENIDSLFATNYNMSSIDCSKLNTTSLKSAAFTFGDCPMLKEVDLS
                     SLDLSKLTDLQGMFSISKVCPTTSILTTIKFPTNTEYLPKSITDSSNMFYGQTNLKTI
                     YTSGN"
     gene            9217..9609
                     /locus_tag="AC04VQ_03240"
     CDS             9217..9609
                     /locus_tag="AC04VQ_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553205.1"
                     /translation="MFYNCINLMGGNGYKYNNSQATDKTYAKFDVYGSNGYFTHSGSA
                     ALIYNMGEHADCDVDHNAEYLNYTQSVPSGYSYSVQGATPLENPVCTDVSGMVFKCWY
                     LDGDETKTEYVAGMELESTGSPQRLIAQ"
     gene            10405..10599
                     /locus_tag="AC04VQ_03245"
     CDS             10405..10599
                     /locus_tag="AC04VQ_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553206.1"
                     /translation="MAANSEQSSMGSIQGSTEIYNNSSHAGTYSINNGVITFTISGTD
                     GNSTTYTSTAEPTSGYQFSK"
     gene            10651..10935
                     /locus_tag="AC04VQ_03250"
     CDS             10651..10935
                     /locus_tag="AC04VQ_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553207.1"
                     /translation="MTFTASFEVAKAAKAVYQKSTTTLTFYYDYESHTGSDYDVYSVP
                     TTNKPVTAFDVLFAPVDLLSTFAVLRPIIEIVYTPINAVIDFAAVVLDLA"
BASE COUNT         3674 a         1852 c         2173 g         3318 t
ORIGIN      
        1 caagaattac ataataactt tcagttcgcc tcgcgctatt gatgcggatg aatatacatt
       61 tactgttacg gctgtagcaa ctggaaacta tgttgggtca tatactggga cttggactat
      121 tttaccggcg ccagttacat attattttga aagtacatga caatatgatg gcgttaaatc
      181 gaacgacaca atttacctaa atcaaaatac tgtacctggt caaatattcc atgcagatga
      241 atatgtgcta acttctgcgg cgcttaataa cggacagcca acaacaaatg ttggagatta
      301 ttattatcct ggtacgtcag aacaaaataa agcaagtatg acgattaaag gaaatatgtc
      361 ttcgttattg agcaattaca catttacaat tacaacgcat cttaagatta caactaatga
      421 tatttcttcg atgtatgagc ttgatgagtt cacaagttca tatacgtata atggttatga
      481 tcaattccca accgatatta aagttgtaaa gaaaggtgat cataccgtag agcttccgaa
      541 gactgaatat acaattgttg gaaataaata taagaatgtt ggagaaaatt atagtttcca
      601 tatagaagct aaagagggaa gtaacttaac aggctctatt tatgcttcaa caagtcctga
      661 tacagacttc acttgagaaa ttacacctac tagtgtttct tatgaattta atgattcaag
      721 aacatataat ggacaaaatt attcaaaaca agttcattta acaagcgcta atggattatt
      781 agttgatgat actatcgatg taacatttgc tgctttcaaa gaaggtagtc ctacaggcga
      841 tgccggcaat tatctatttg aaaatgagac tcttagaggc tcatatagca acagtaacta
      901 tacgctaaca tttagtgggc aagtaattat taacggaaag agtattaaag gccaagaaat
      961 agatattgag tatactcatg atcttgaata taatgcaaac gagcaaagtc ctactgattt
     1021 aactgacttt aaagttaagg atactactct tggtcaggaa ttgtctctaa ctgatgatta
     1081 tgttgttaca tcaagtaaac agactgatgt tcctgaatcc ggagtttata catttacaat
     1141 tcaaggtgtt ggaaattatg ttgattctat aagtaaagag tgaacaatta taccgcacaa
     1201 tgtttcagtt agtggactta ccaaaaccct atcttataat ggcttaaatc aaactcttga
     1261 aaaaacttta agtactacgg atggcttata taatgaagat caaattaaat taacatttac
     1321 cacaactggt ccaaatgttg gtaaatatga atatccaagt actgggttga agagcttaac
     1381 attaagtaag gaaagagttg agggaactgc ttatgttctt cgtgattaca catttgatat
     1441 tttaaatgtc tgagtacaaa ttaatccgca agaacttagt ggcgatttta atgttcaggg
     1501 cgtcaatgat ttaacttaca atggttataa gcaaaccttg aatttaacaa ttgaaaaaag
     1561 tgatgggtct attgtattaa ctccgcttga ttatgacgtt attggatcaa tttctgaaaa
     1621 agatgttcct gatcctaagg tttctgatca ttatgagttc catattaaag gaaagggaaa
     1681 ctttagtgga gaaatttgga atggttcacc taatgtacca tttacatgga aggttaaacc
     1741 tgcttctgtg acatataact atgttgatcc acaaagtcct ttaacttata acgctgaaaa
     1801 atatgaaaat gatgttgtaa ttggcgaagg aacattattt agcactgacg aaattggtct
     1861 taatgttgtt tctgattcaa agaatgtagg aacatatagt ttcccaacta ctaatgctga
     1921 tggaaacttg attccaacgc taagtgagat taatactccg cttcgcaatt atgatttgac
     1981 tttaactggg tcaatagtaa ttaataagaa gcaaattaat ccggaagata ttattcctgt
     2041 ttgtcctgag tataatggtg atgaacaaag tcctgtaatt gttattaagg ataaggacac
     2101 tggtgaggcc ctaatttcgg gtgatgacta tactttgagt ggaataaccg aagcaactga
     2161 tgttgctgct acagacatga ttattaatgc aacacttgat ccaggtgcaa attattctgg
     2221 aattattcct gcaattcctt ggaacattag gcctaaggcg attacaatca agattactga
     2281 agaaaaggtc tttgatggta agacaattat agtcgaagga acattgccaa caggtgtcta
     2341 tgaacaatca tttgattatt cttatgaaac tgaggattct gctgttggtg tttatagcag
     2401 agagaaaggt aatttgatag gtggttacga accaactgat gaagaaacta aagttactaa
     2461 ttatgcagta aacgaagatg ttgttttaac tatcaatggg gttccaccag agccttcgcc
     2521 ggatgtccca gaatatgttc ctggacagac tggtgatagt acgccggtag ctggatttgc
     2581 gcttatggca ttaacactaa ttggcgcatt cttgttcttg cgcagaccga agaaagtcca
     2641 aggattgcat attcgataaa cgtcaaagtt agctcagagt agatttcaga ttaaaccccg
     2701 gggccaaaac ctcggggttt tttgtaaaaa tttatcggca aaatttgtag atatcttgaa
     2761 ggatttttaa ctttttgccg ataaaatgtt ataataggta cgttaggagc agttatggat
     2821 tataaaacag ttgcagaaat agcaaaaatt tggggaatta gcgagcgtag cgttcgaaat
     2881 tactgtgctc acaatcgtat tcctggagca atacttaaag gtaaaaaatg atatttgcct
     2941 gcggattcga caaagcctcg ccgattaaat gcaagtgata gtcctaaaaa ttatttatta
     3001 gaaaggttgc ttgaggaaaa aagaagcaag atttcgggcg ggatttatca taaaactcaa
     3061 attgatctaa cttataactc gaaccacatt gagggaagta ggttaactca cgatcaaact
     3121 cgctatattt ttgagaccaa cactatagga attgaaaatg acgatattaa tgttgacgat
     3181 gttgttgaga caactaacca ttttcgctgt attgacttta tcattgataa agcgaattga
     3241 aatttgactg aaagttttat caaggagttg catttaattt tgaagactgg taccagcgat
     3301 gcgaataagg attgatttgc tgttggagat tataaaaaaa tgccaaatga agttggtgga
     3361 atgcagacta ctcatcctga aaatgtaggt tttgagattc gaaagttagt taacgaatac
     3421 aattcaaatg ataacattca gcttgacgac attttaaatt ttcatgtgcg atttgaagga
     3481 attcatccat ttcaggatgg taatggacgt gttgggcgat tgattatgtt taaagaatgc
     3541 cttaaaaata atattgttcc gtttataatt gaagatgaaa tcaaaatgta ttattatcgt
     3601 ggccttaaag aatgggataa cgaaaagggt taccttcgcg acacttgctt aaatgctcaa
     3661 gataaatata aagagaattt aaattatttt gagatcaaat attctggata agcatctgag
     3721 catcaagcat tataaaataa ataaaaatta actgataata aaaaatatta aactcccgct
     3781 gcccccggcc cccataagct acaatgtggg aaaagtgata taattatagg tttctaatat
     3841 gagaaagatg ttagggtggt gagaattatg agcgttgaaa cattaataag gtttgttgct
     3901 gtaacgcctg gagggggcgg cggcgatgac ccaattatca atcctggtag ttggaatgct
     3961 caaacaggtg atatgtttgg aatgataatt gcaattttgc ttgcaattgc atgtattgcg
     4021 gcgtttgcgc ttcgtgccta ttcgaaatct cgcgtttatg caggctccga agccaaggcc
     4081 tcggcttcca catcttttaa agggttaaga acagccatgg ctgtaattat tgctgctttg
     4141 gcatgcgcaa tgcttgtaaa tgtaacgagt atgcaagttg ctgctaaaca gcaaactgca
     4201 aacggcgaaa ttacttcgcc agcaagcatt gaagcgcacg ttgatgaaac tgctggaaca
     4261 atcacaattg acaacagcta ttttatgaac gggtcggaca ccgattcggt gcttaattca
     4321 ttaaccgttt caagcgcaat ggaagtaaac gatggcggcg caacttgaac aatttatggt
     4381 ttgaacagca ctctttacaa cgacgtcatt ggcaaaaccg tcacttttac cgacacaaaa
     4441 acaattgccg ctggcactaa ggaagacata aagatctcga caaacatgga catcgcaacg
     4501 gcaaagggtc taattggcca aaatgtcttg aagatgagct ggacatattc agattcgcca
     4561 acacctccac cacctccgcc tcctcctgga gagcaaattg aaatcaaggg tgttgctgtt
     4621 gatgcggatg gtcttccaat tgcaaatgcc acggttacta tttatgacga agccggcaac
     4681 gcaattgcaa ctgcaactac agacgcaagc ggaaactaca cgttgacttt gccagaagat
     4741 atgtctaagc tggacaaagg cgtcattaaa gtcaagacaa gtggcggagt ttcgcaagac
     4801 ataattgttg aaaacatcac gcaagacacc accttgaccg aaaagtcaac cgtggagcct
     4861 agcacaacaa tttctggcac cataactgac gaagacggaa accctgttgc tggtgttgaa
     4921 gtaacctata caactgtggt cacttaccca gacggaacaa caaaggttga aacttttact
     4981 ggcattacgg atccaaacgg caaatataca attgatgtta agcaagatca taactatatt
     5041 gaaaatgtcg acaaaaagac ggagcctcaa attcacacgt ctgacaaaat cgaggttgaa
     5101 aagactccaa tttcaaatga catcgaagtc acagaaactc tctacactgt tacggttaac
     5161 ttaggaaacg gaagtgttct atacgttccg gaaggttgga cttcgtcaac tgctggagtt
     5221 tacacgaaag atttaaataa aaattctgac atcccttcaa tcattgcaat gtgagaaggc
     5281 ctgggcatta atccaccaag tggaaaagct ttcacaggct tgacttcgga ttcgtcaaca
     5341 gatgccttgc ttaagcctac attaattaag gctagctatg atgcgcacgc ttacataaat
     5401 ggtaaagccc tcgacgaaga tggccaacct ctttatcctg gtaaggctgt ttttattgat
     5461 gaaaatggaa acactacagc ggttactctt aacgaagacg gcacatattc gcttccaatt
     5521 gatccaaaca cgtcaggcac ttttcatatt gagagcactg atggagaata ttcgccggac
     5581 tataaaatta gtggtgacga aggtgtgacc aacatccccg aaacttacgt tgagccttcg
     5641 acaaaaattt ctggaacagt ttatgggccg gatggcaagc ctttaaagaa tactgaaatt
     5701 gaaattacaa cttatgttga gcgcgatggt aaaactgtcg aggaaaaata cattgtaaag
     5761 actgacgaaa acggtcatta tgaaaagaat gttcaacaaa ataaggacta cacaattaag
     5821 gttccaattc cggacacaaa tcctccagtt aattatgaag aggatattga ggttcttaca
     5881 actgaggttg aagttccaaa aattgatgtt ccaagcgcaa ccgaaactta tgttgcaaca
     5941 attaatttga acaaaggaac aattccacgt gcgttaagcg gctggcaaaa aactacagct
     6001 ggaacttata caaaaacttt tgctaaaaat tcaacctacg aagccattac ttcgtcttga
     6061 acaaatgcaa atgttacgcc tccagtttcc ggtcaagtaa ttacaggttg gaaccctgct
     6121 tcggggacct tggttgcaaa tacaaccttc actgcaaatt gagggtcgcc aacgcctcct
     6181 cctccggttg gaaattatat agttggaact gttgttgacg aatttggaaa cgttgttcct
     6241 ggagttgaca ctacttttac tgatgacgat ggaaaaacct acacagcgaa gaccgatcaa
     6301 catggtatat ataagattgg tccaattcca gatgacactc atggcacctt acaattccgc
     6361 gacaccactt cgacgcctcc aaaaattgga ccttctcaag aaattactat tgatggtgaa
     6421 attgtagaag cggataaatc tgttgttgag acaagtcata aaactactgg gaaagttacg
     6481 gatgcaacag gcgatccaat tgctggtgca gatgtaactt ttcaaactac aacttatgtg
     6541 gatggagaaa aagtcattga aacctacaca acaaaaacta atagcagcgg tgaatataca
     6601 attgaaataa aagaaaacaa aaaatacgat gttgaaatta aaaaggaatc taatccaaaa
     6661 attgacttta tacagtcagt tgaggttgtt cctggtgata caagtccaat taatgttgat
     6721 attccaaaag gacaaatggc aacatttagt gttgatcttg gcgaaggcgt aattttacct
     6781 gaagatgttc ctgctggttg gactaagtca accgaaggag ttaacactta cgaaaagcaa
     6841 atgcttcaat caaaacaaac aatggaagct gttataaaat tatgaactac ggccaagatt
     6901 gttcctcctg aatctaaagt gttaagtgag atcatttttg aaaccgatac tcattacaag
     6961 gcaaagtatt cccctcttaa gacaatttct ggtcatattt tagacgaaga cggaagccct
     7021 gttcctggtt cagttgttaa gttgtatgac tccgaaggaa acgttgttga tgaaacaaca
     7081 actgataata atggtcaata tgaatttaac gttgcccaag gcaatgaata taagataaag
     7141 gcaaatactc caactggaac tacaacagaa tatgatgttg gtaaagttga agaagacatg
     7201 aatcttccta acatcgaaat ttttccaaaa gctaaacttc atggacaaat tacggattct
     7261 gaaggtgttg gcatgtgata tgttgatgtt aaattgtcat ataaattaga agacggaaca
     7321 aaaattcaat ccagtgcagt taccaacaag aatggatttt atagtgttga acttccaagt
     7381 ggctttaatt atgatattga gattttgggc tattcttatg gcacttatgt tggtgaacta
     7441 agagttgatc aagtcgatca aacttttaat tatcaatttg gtatttataa aacagcaggt
     7501 gttttacttt ataacgtttt ttcaagtggg gggacagaac atccagatgg ttggtctgtt
     7561 agttcaagtg gctgagaaaa gtcatttgta gttggctcaa gcattgctgg aattttacat
     7621 gaatgggatg gctatgatat tcttcctcct gatgaaaatg tagataaggt tttttcagga
     7681 tgagataaat caaggggaac attgcaagga gatattaaaa ttagtgctat ttgaaaatgg
     7741 ccgataactt tcagctctag cccttcgaca tatggaaatg tgacacttaa tccagcaact
     7801 tttgatggac ttgtgcctgt tggtaatagt tcctatgaag ttttggaaga caattcaatt
     7861 aagattgtta ctgaagccac tgtattagtt gttgctgctc cagttcctgc ttcggattca
     7921 tatacttttg atgggtgaac agttaattca caaagcattg cgccaaagga tatgattcaa
     7981 ggcaagatgg acttcgttgc taattttaga ttgcttggta atatgttccc gaaagctgta
     8041 tttaaagcgt ccgatagcag ttttactttt tattatgatg atattgatca tagtatagat
     8101 gctgccgatg gggatgttca atatatttta gaaaaggaca cgcatttaga ttttaatgat
     8161 ctgagtacaa cggtttatgc accttgacat aatgttgatc caacttatgg cattcctata
     8221 ccaaaagtta accctacaaa agttgttttc gataaaagct tcaaaaattt tacacaatat
     8281 aatgactata aaactaatac aacaacacca tttaagagca tggatggatg gttctttaat
     8341 atgataaaag ctcaatctta ttctgggttt gaaaatattg ctgattgtgt agaaagttgc
     8401 aatacaactt tttgtaattt tgggggcatt ggtgatgaat taaacgaaat ggatgttcat
     8461 aatgcttctt tagatttgac aaattttcat caatcaaaat tggcaaatgt gcaatctatg
     8521 tttagcacat gtttgtttaa cgagctaata attccagata agtttgcacc aaaggcaaca
     8581 agttttggag gtgttgtaac acttgggcct gatcaagcaa ttgtaacagg tatgtttagc
     8641 gctgctttgt gtaacaaaat tattattaat ggaactattg ggacaggagt aacagatttc
     8701 actggtcttt tcactcaatg cacaaatatt aagactcagg aggacgcaag cattgataaa
     8761 gtagactatt cgagcgcagt tgatgttacg caggccttcg ggggtttaag ttttgacaag
     8821 tttgttcttc cgacaagttt taagaatgca acgaagttag aaaatattga tagtcttttt
     8881 gctactaatt acaatatgtc gagtattgat tgttccaagt tgaatacaac ttcgttaaaa
     8941 agtgctgcat ttacatttgg tgattgtcca atgttaaaag aagttgatct ttcttcatta
     9001 gatttatcta aactaaccga ccttcaagga atgttttcta tttcaaaagt ttgtcctaca
     9061 acgtccattc ttacgactat aaaatttcca acaaatactg aatatttacc aaaaagtatt
     9121 actgatagtt ctaatatgtt ttacggtcaa acaaacttga aaacaattta tacaagtgga
     9181 aattgaggtt tttcaagtgg agctgtatct acttctatgt tttacaattg tattaattta
     9241 atgggaggca atggatataa atataataat tcacaagcaa cagataaaac atacgcaaag
     9301 tttgatgttt atgggtcaaa tggatatttt actcatagtg gaagtgcagc tcttatttac
     9361 aatatgggtg aacatgccga ttgtgacgtt gatcacaatg ctgagtattt aaattataca
     9421 caaagcgtgc catctggtta tagttattcg gtgcaaggtg ctacgccact tgagaaccct
     9481 gtatgcactg acgtcagtgg catggtgttt aaatgttggt atcttgacgg tgacgaaact
     9541 aagacagaat acgtcgctgg aatggaactt gaatcaacag gatctccaca aagacttatc
     9601 gcccaatgac aagaagcacc agctgttcct acggcggtgt atgaaaattc tacgtgtgaa
     9661 aaaaataaag gaactctaaa attttatcgt gataaagaac acgtaccaag tactgaagcg
     9721 tgaaaatcaa ccgacacatc atatgttttt gctcttccgc ctgaaaccta ttcttcgcaa
     9781 aatgatattc cgtggcatgt tgcttatgaa tatataggca cagtttcatt cgattcaagt
     9841 tttaaaaatt atcaattaac aaatcttagt tattggtttt atgatttgca tatttcaaca
     9901 ataaattgag aatattctgg cacggatgat ttacttgata cgtctaaagt taaaaatatg
     9961 aaagatgtgt ttgcacatac ctctaaactt caagaaatgg tttttccatc tcttgatttt
    10021 agagaagttg acacttttaa tagttttctt aattatagcg gcataacaag agttgatttt
    10081 tctaatatca tttcttgtga aaaaactgat ccttcctatt gaccaagtgc agcgtatgca
    10141 ttacaatcgt gtcgcaattt aaaagaagtt aatcttacca actttaaagt ttattatatt
    10201 tatgattttt tcaatagttc aaataacatt gaaactataa ttattgatga aaactgacgc
    10261 tcaaatttta atgttttacg gattaatgat tcttattgaa ttgatgagaa tggcaaatca
    10321 tatcattatg atgaagtgcc aaatcataat gattcgagta aacatgtatg aggtaaattt
    10381 acaaaagcta caaataaaac tacggtggct gcaaattctg aacaatcaag tatggggtct
    10441 attcaaggta gcactgaaat ttacaacaac agttctcatg caggcacata ttctataaat
    10501 aatggtgtaa ttacatttac aatttcagga acagacggaa attcaacaac ctacacaagc
    10561 acagctgagc caacttctgg ctatcaattt tctaaatgac agctaaatgg gtccgatatc
    10621 acttccacaa caggaccttt aactgataac atgacattca cagcaagttt cgaagtagct
    10681 aaagcagcca aagctgtata tcagaaatct actacaacct taacttttta ttatgattat
    10741 gagagtcata ccggttctga ttatgatgtt tattctgttc caacaacaaa taaacctgtt
    10801 actgcttttg atgtgctgtt tgctccagtt gatttgcttt caacttttgc tgtacttcgt
    10861 cccataattg agattgttta cacgccgatt aatgcggtga ttgactttgc tgctgttgtc
    10921 ctcgaccttg cttaattgct gaagaagcag aaaatagttg taattgatgg gtataacgtt
    10981 ctccggaatt cggagcgtta tgcccatttt gttgggg
//
LOCUS       DBNCDC010000028        10222 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_2743_length_10222_cov_6.451559, whole genome shotgun sequence.
ACCESSION   DBNCDC010000028 DBNCDC010000000
VERSION     DBNCDC010000028.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 10222)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10222)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10222
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2743_length_10222_cov_6.451559"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(51..209)
                     /locus_tag="AC04VQ_03255"
     CDS             complement(51..209)
                     /locus_tag="AC04VQ_03255"
                     /inference="COORDINATES: protein motif:HMM:NF022779.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HIO9553208.1"
                     /translation="METENVTFNKKFRVHAEDAETAFYICTPKFMDKLVEFSNVYKRG
                     LLLSFKDN"
     gene            complement(222..662)
                     /locus_tag="AC04VQ_03260"
     CDS             complement(222..662)
                     /locus_tag="AC04VQ_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553209.1"
                     /translation="MESFMEMFGGYIIPIIVIIVVIIIVAVSKSKQKKYKNEVTSQII
                     KEMFPDADYRPDEYISRDEYDEIGLRRGSYFEGSDLLYATTKGGRKFRFCEINTYDKT
                     HTDKSTTTVTIFRGGIFSFEYPKKFTSAVAIEKKGFNAAGSLKT"
     gene            734..1234
                     /locus_tag="AC04VQ_03265"
     CDS             734..1234
                     /locus_tag="AC04VQ_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family phosphohydrolase"
                     /protein_id="HIO9553210.1"
                     /translation="MELAITRDQALELLKKYNKEEFHLRHAYTVEGVMRWYANELGYA
                     VDEEFWGLVGLLHDVDFEEFPEEHTLKAPELLAEIGCSEDMVHAIVCHSYGHFSDVPP
                     EHQMEKVLYAADELTGLIWAYALMRPEKNCVEMKVKSLKKKFKDLKFAAGCDREVITK
                     GAEMCG"
     gene            complement(1310..1447)
                     /locus_tag="AC04VQ_03270"
     CDS             complement(1310..1447)
                     /locus_tag="AC04VQ_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553211.1"
                     /translation="MLNAQNVDEYGKNKKVMDPASFTIFEDVVNNEILTDLSPSEILL
                     K"
     gene            complement(1589..1807)
                     /locus_tag="AC04VQ_03275"
     CDS             complement(1589..1807)
                     /locus_tag="AC04VQ_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553212.1"
                     /translation="MDPLYLATTSTLLGQIKAYNESTCDEELAYRIDCEELNSIKVNS
                     NFLAKMNLAANLSFLIISYIKAKQQVYE"
     gene            complement(1859..2299)
                     /locus_tag="AC04VQ_03280"
     CDS             complement(1859..2299)
                     /locus_tag="AC04VQ_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553213.1"
                     /translation="MNELIQPTVSALIDAFDNYQITNEALAAEVDAFKRELIDFAEQN
                     DDPATFFDAYQKSGVQDKYTALITKVAMAGQTGADASQDTIQNEAELPSVSEFLEQYR
                     ASYDEVCKRDSVAGKRAYEELFDIANKADDLLEAQIIIEETRIL"
     gene            complement(2306..3097)
                     /locus_tag="AC04VQ_03285"
     CDS             complement(2306..3097)
                     /locus_tag="AC04VQ_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553214.1"
                     /translation="MSSLENSIKLYKLMRDRCAASGFCEHQAKVYAEMVDFIAACDSV
                     EDSMQKIKNSKYYLAPSAALLKDKLEAEKKFATHHDMQDLADVYDAKIREIDEDVNNM
                     YTNDYSSKAQTIKSNYVNSLTAFGNVFVAYVTYLCSGEDTHITDIKKQAGYIKYPASD
                     FAEVAKLEIYQNLVPLNVDAYKKFVSDVREILNGGNVESFADIANEDAFNAEVEFDTL
                     SSKKEELKTKGEKWLSLSFENTHIAHAPTYDASKDISNLKYEYEG"
     gene            complement(3097..4293)
                     /locus_tag="AC04VQ_03290"
     CDS             complement(3097..4293)
                     /locus_tag="AC04VQ_03290"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016774 - phosphotransferase activity,
                     carboxyl group as acceptor [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HIO9553215.1"
                     /translation="MKVLVINCGSSSLKYQLIEMDDESVLAKGLCERIGIDGSVLTHT
                     KTGQDKVVIEEPMPSHKEAVQLVFDALLDAEHGVIKSMDEVYAIGHRIVHGGNVFTDS
                     IIVTEKEKELINEVSDLAPLHNPANLLGIEACEKVAPGKKNVAVWDTAFGMGMDPKSY
                     LYAIPYELYSKYGIRKYGFHGTSHKYVTGEVIKLAELPENSAKVIVCHLGNGASVSCS
                     IGGKCVDTSMGLTPLDGLIMGTRSGTIDPGAVQYMCRKLDIDIDKCIDILNKESGVLG
                     LSGGVSSDFRDIGEAADGGNEYAKVALEAYWYRVAGYIGQYVAYMNGVDAIAFTAGVG
                     ENDINARKQICSYLTYLGVEIDEAANDVRGEVRQISTDNSKVKVYIYPTNEELAIARE
                     TLRLVK"
     gene            4649..5110
                     /locus_tag="AC04VQ_03295"
     CDS             4649..5110
                     /locus_tag="AC04VQ_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553216.1"
                     /translation="MNHPEVIGFDVYASLLKPTSCMVNYLQSQKLIAPTTILNILRGA
                     LNFPVEEACHLNNISVNDISFLSCERTFDNNGDINGLAVKYEKIDAVPNATLMIGDIL
                     ASGETLIECLRHVTDYYRQNNERLKNIILFTIGGSRAIPLLEDLTNELRGY"
     gene            complement(5668..5877)
                     /locus_tag="AC04VQ_03300"
     CDS             complement(5668..5877)
                     /locus_tag="AC04VQ_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553217.1"
                     /translation="MEVGKIANMTIVDTDLIHEDINALPLAQIVATVVDGHEVHNSAD
                     DPYAKDRKQNPQDYIRNFFYSKKYD"
     gene            complement(5902..7410)
                     /locus_tag="AC04VQ_03305"
                     /pseudo
     CDS             complement(5902..7410)
                     /locus_tag="AC04VQ_03305"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
     gene            complement(7856..8530)
                     /locus_tag="AC04VQ_03310"
     CDS             complement(7856..8530)
                     /locus_tag="AC04VQ_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIO9553218.1"
                     /translation="MIIYCMSDIHGFIDEFKENLSIIADNLKCEDSKLILLGDYIHGG
                     NGGYEVCNAIIRLQEKYGTDKVIALLGNHERWCLEGRMPVDESSSFNEKFESVLLDWF
                     SELPLYHVIENNIFVHAGIDEEAGDLWELGTNDYYFTDKYPADIGRVEGLDKNIIAGH
                     IGTWDIAKNDTFHDIYYDGYCHYYIDGTTYVSSIIPMLKIDTTKGTYKEFIRSHWNDV
                     LPYNKL"
     gene            complement(8935..9456)
                     /locus_tag="AC04VQ_03315"
     CDS             complement(8935..9456)
                     /locus_tag="AC04VQ_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553219.1"
                     /translation="MDLIPQFLKDYYLSNRITISDKLKASIICNSKALDYRQKINKLT
                     ELAKGDIHPTIKNQIENNIEDINTAYSTFCCNNGKEYRYVVADKQGNPLLVFECFPMV
                     ENFLNLKKSENNNEYENIFVKLYKTENCKEMLNNNDTQSIQIFDNKDVSYIEVKNFTD
                     YVYIKNELSDYID"
     gene            complement(9609..9860)
                     /locus_tag="AC04VQ_03320"
     CDS             complement(9609..9860)
                     /locus_tag="AC04VQ_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553220.1"
                     /translation="MHKFDNLINNYIVSLVREDLDVVTGNAHHNFTEIQIVKNLYGSP
                     EIGGAYNPSPKKDGDNYIIKHQFLYSYPCIVSKENAEKP"
BASE COUNT         3218 a         2045 c         1552 g         3407 t
ORIGIN      
        1 ccaaacattg gagctttaaa catatccaaa gttggaattg caatatgaac tcaattatct
       61 ttaaagctta aaagcaaacc tcgcttataa acattgctaa attcaaccaa cttatccata
      121 aactttggag tacaaatata aaatgcagtt tcagcatcct cagcgtgaac acgaaacttc
      181 ttattaaatg taacgttttc ggtctctatc tttttctcgc ctcatgtttt tagcgaacca
      241 gcagcattaa agcccttctt ttcaattgca acagcggaag tgaacttctt cggatactca
      301 aatgaaaaaa tgcctccacg gaaaatcgtt acggtagttg tagatttatc agtgtgtgtt
      361 ttatcataag tgttaatttc gcaaaaacga aacttcctac ctccctttgt agttgcatac
      421 aaaagatcgc ttccttcaaa ataagaaccg cggcgaagcc caatttcatc gtactcatct
      481 cttgaaatat attcatctgg cctgtaatct gcatcaggaa acatctcttt aataatttgt
      541 gaagttactt cgtttttata ttttttttgc tttgattttg aaactgcaac aataatgatt
      601 acaacaataa taacaataat cggaataata tatccaccaa acatctccat aaaactttcc
      661 ataattcctc cttgcctaaa taaattataa cattgaatat gctaaaataa atctcaaatg
      721 aacgggggtt gttatggaac tagcaattac gagagatcaa gcgctagaac ttttgaaaaa
      781 atacaacaag gaagaatttc atcttcgtca tgcttatact gttgagggtg taatgcgttg
      841 gtatgcaaac gaacttggtt acgccgtaga cgaggagttt tggggtcttg taggacttct
      901 tcacgatgtt gactttgaag agttcccaga agaacatacg ctaaaggcac ctgagctatt
      961 ggctgaaatt ggatgttccg aagacatggt ccacgcgatt gtttgtcatt cttatggtca
     1021 tttttcagat gtgcctcctg aacaccaaat ggaaaaggtt ttatacgcgg cagatgaatt
     1081 aactggccta atttgggctt atgctttaat gcgcccagag aaaaattgcg ttgagatgaa
     1141 agtaaaaagc ttaaagaaaa agtttaaaga tctaaagttt gctgctgggt gtgatcgcga
     1201 agttataact aaaggtgcag aaatgtgtgg ttgagaactt tccgagctct tcgaaaaaac
     1261 cattgccgcc atgcaatcct gcgaagaacg agttaacgaa caggttaatt tactttagta
     1321 aaatttccga agggctcaag tccgttaaaa tttcattatt gacaacatct tcaaagatag
     1381 taaaagaagc tgggtccatg acctttttgt ttttgccata ttcatcaaca ttttgtgcat
     1441 ttaacattca aatttttacg ccttcatcat tcattaaatc agcaaaggaa tagcctatcg
     1501 atttttccat aaatttcaaa agctgctgaa tcttaatttt gaaattatgc atagcaatta
     1561 ggccatcttt gacaagctta tccttccttc attcataaac ttgttgcttc gccttaatgt
     1621 atgagattat taaaaatgaa aggttggcag ccaagttcat cttcgctagg aaatttgaat
     1681 taactttaat ggaatttaac tcttcgcaat ctatacgata agccaattct tcatcacaag
     1741 ttgattcgtt ataagcctta atttgaccaa gcaaagtcga agttgttgca aggtaaagtg
     1801 ggtccatctc ctcgagcttt ttttcgaaaa tttcaacagc atcgtcctta acaatccttc
     1861 aaagaattct ggtttcttca ataataattt gggcctcaag caagtcatcc gccttgttcg
     1921 caatatcaaa tagctcttcg tatgctcgct tgcctgcaac actatcacgc ttacaaactt
     1981 catcatacga agccctgtac tgttctaaaa attcactgac acttggtagc tctgcctcgt
     2041 tttgaattgt atcttgcgaa gcatccgccc cagtttgacc agccatcgca accttcgtaa
     2101 ttaacgcagt atatttgtcc tgaaccccgc tcttttgata agcatcaaaa aatgttgcag
     2161 gatcatcatt ttgctcggca aaatcaataa gctcacgctt gaaagcatca acttcagcag
     2221 ccaaggcttc gtttgttatt tgataattat caaaagcatc tatcaaagca gaaactgtag
     2281 gctgtattaa ttcgttcata accacctacc cttcatactc gtactttaaa ttgcttatgt
     2341 ctttgcttgc atcataagtt ggcgcatgag caatgtgagt attttcaaaa gaaagagaaa
     2401 gccatttttc gcccttagtt ttaagttctt cctttttaga acttaaagta tcaaattcca
     2461 cttcggcatt aaaagcatct tcatttgcaa tatcagcgaa gctctcgaca tttcccccat
     2521 ttaaaatttc acgcacatca ctgacaaatt ttttgtaggc atcaacattt agaggaacca
     2581 aattttgata aatttcaagc tttgcaactt cagcaaaatc agatgcagga tacttaatat
     2641 atcccgcctg ttttttaatg tcggttatgt gagtgtcttc gccagaacaa agatatgtaa
     2701 cgtaggcaac aaaaacattt ccaaaagcag tcaagctgtt tacgtaattt gactttatgg
     2761 tttgagcctt actgctgtaa tcgttcgtgt acatgttgtt aacatcttcg tcaatctcgc
     2821 gaatcttagc atcgtaaaca tctgccaaat cttgcatatc gtgatgagtg gcaaatttct
     2881 tttcagcctc aagtttgtct ttaagcaagg cggcacttgg ggccaagtag tatttggaat
     2941 ttttgatttt ttgcattgaa tcttctacag aatcgcacgc tgcaataaaa tctaccattt
     3001 cagcgtaaac ttttgcttga tgctcacaaa accccgaagc agcacacctg tcccgcatca
     3061 atttatataa cttaatagaa ttttctaaac ttgacattat ttaactaatc gaagtgtttc
     3121 gcgagcgatt gcaagctctt cgttagtagg ataaatataa actttaacct tactgttatc
     3181 tgtagaaatt tgacgaactt cgccgcgaac atcgtttgcg gcttcgtcaa tttcaacacc
     3241 aagataagtt aagtatgaac aaatttgctt tcttgcattg atgtcgtttt cgccaacgcc
     3301 agcagtaaat gcaattgcat ccactccatt catgtaagcg acatattgtc caatgtatcc
     3361 agcaactcta taccaataag cttcgagcgc tacttttgca tattcattgc cgccgtcagc
     3421 agcctctcca atatctctaa agtccgaaga cacgccgcca gaaagaccca aaacgccaga
     3481 ctctttattc aaaatgtcaa tgcatttatc gatgtcgatg tccaatttac ggcacatata
     3541 ttgaacagca ccaggatcaa ttgttccact tcgtgttccc ataataagac catcaagcgg
     3601 agttaatccc atagaagtgt caacgcattt gccaccaata gagcaagaaa ccgaagcccc
     3661 attacccaag tggcaaacaa taacttttgc agaattctct ggtaattcag ctaatttaat
     3721 aacttcgcct gtaacatatt tgtggcttgt gccgtggaag ccatattttc taattccgta
     3781 ctttgaataa agttcataag gaattgcata taaataagat tttggatcca tgcccattcc
     3841 aaatgcggta tcccaaacag caacattttt cttgcccggg gctacctttt cgcaagcttc
     3901 aataccaagc aagttagctg gattgtgaag tggagctaaa tccgaaacct cattaatgag
     3961 ttctttttct ttttcagtaa caataattga atctgtaaat acgttgccgc catgaacaat
     4021 cctatgacca atagcataaa cctcatccat agacttaatt acaccatgtt cagcatcaag
     4081 cagcgcgtca aacacaagtt gaactgcttc tttgtgagat ggcattggtt cttcaattac
     4141 aactttgtcc tgtcctgttt ttgtatgagt taaaacagat ccatcaattc caattcgttc
     4201 gcataagcct ttagccaaaa cagattcgtc atccatctca attaactgat acttaagcga
     4261 tgaacttcca caattaataa ctagtacttt cataatttaa taacctttct aatataacca
     4321 gtttttttaa gaggccttta tccgctcctc aatttttatt ctatgttaga tttgtaaaat
     4381 ccgcatgtat attttatatt tatagaatac tatatcttat aaaagtttac aagaaaaaaa
     4441 ttttgaaact atcaaaaaca tatgttctct taggtgttat aatgatttca tcaagtttat
     4501 aaaggctcaa gttaaaatga tcgagaattg aaatagtgtt caaattacct cagaattttc
     4561 atcccaagat gtagattgct acaagttaag cggaagcaat tttattaata attattatat
     4621 tatttctgca gaaagtacaa gaaatttgat gaatcatcca gaggttattg gctttgacgt
     4681 ctacgcctca cttctaaaac ccacatcttg catggttaat tatcttcaat cgcaaaaact
     4741 tattgcacct acaaccattc ttaatatttt acgaggcgct ctaaactttc ctgttgaaga
     4801 agcttgccat ctaaataata tctctgttaa cgatattagc tttttatctt gcgagcgtac
     4861 attcgacaat aatggcgaca tcaatggtct tgctgttaaa tacgaaaaga ttgacgctgt
     4921 ccccaatgca acacttatga ttggagacat tttggcttcg ggggaaacac tgattgaatg
     4981 ccttcgtcat gtcacagatt attaccgtca aaataatgag agactcaaaa atattatttt
     5041 attcacaatt ggaggaagta gagctattcc tcttcttgaa gatcttacta atgaattacg
     5101 aggctattga ccagatttcg aaggctttat tactgttttt tacgaaggaa tttttagctg
     5161 ctacgaacct ggtgattatg gagtatcggg aattaaccgc cctctgattg atttcatttg
     5221 aagagacggg attatctctc cggaatttag acgtcaaact ctttcgataa gagatgtcct
     5281 atttgaaaaa tgtatcatct acgatggtgg cgctcgaaga tttaatatca aagaacacat
     5341 tggtgaagtt cttgaatttt gaaatggaat tcttgagcat tcagataaaa ttaatatgca
     5401 aaaactgctt gaagaaaaac taggtcacaa acttccaata tcgttcgaag attgatgtga
     5461 cagttgccac tataatagtc ttcccgaaga cgaagccaaa accctctatc aacaagaact
     5521 caagtttatt gagagcctaa acggagtaac gcttaaagat atagccaaca agcgcatcga
     5581 agaattcact tcggctctaa aaaattattc gatttaacaa ctgtataatt attcttctaa
     5641 agttgcgtat caatcaaccg tgtcatctca gtcatatttc tttgaataga agaaatttct
     5701 aatgtagtct tgtgggtttt gttttctatc cttagcataa ggatcatcag ctgagttatg
     5761 aacctcgtga ccatcaacta cagttgcaac tatttgtgca agcggcagag cgttaatgtc
     5821 ttcgtgaatt aaatccgtgt caactatagt catgttagca attttgccaa cttccaatga
     5881 accaagcttg tcttcttggt gtcacatata tgcaacattt tttgtaagac cagctaaagc
     5941 ttgctccctg gtgataattt cgttagcgtt acttacgctg tcaggaccgc ctatgccttc
     6001 aacaattcca cttggaaggc tacgagttac agctgtataa atgctcattg ggccattgaa
     6061 tgacggactt acaggataat ctgtgtggaa tgccgcatca actcctgcat cgtaaaatga
     6121 cttaattggg tatgacttat ctaccttttc tgcaccaaca gaagtacatt ctgtttcata
     6181 ggcgactgga ttttttggag accaaagtgg aggaacaacc gcaattgtat ttgtgtcagc
     6241 aaatcgttga acgtcttcct gtcgaacaaa atgaagatga gcagccacgt tcctttggtc
     6301 tttatttcca gaaatctttt gcgcctcttc aatacaatca aggaagaatt ttgtagcgcc
     6361 atcaccttca gaatgagaat gaactgccaa gttgtatttt ccggcttcgg caataagttc
     6421 tatcattttg tctttattgt taaagcgctc aagcccatgg tatccagcct gatctaaata
     6481 atcactggtc aaccaggatg ttcgagcttc caaaacacca tccaaaaaaa ctttagcacc
     6541 aattacattg aaatattcac cattatgatc ttgtgcatac cttacaattt gtgcaattct
     6601 ttctttagga tcatcaacgt tatctgcaac cattaaatat gcataggttc gcattttaag
     6661 tttattttct ttttgtaatt cctcctgtgc ttcagtagca tttttagaaa taagctcagt
     6721 gccagcatcg gccacagccg tgtaaccatt tgcaaacgcg aaatcttgga aatttagaac
     6781 ataatctttg gcatctttga cactaacttc tatgcttgat aaaattttga ttgcaggatt
     6841 ctcacacaca taaccatcgg gattaccact tgcatctaca tgaacaagtt caggccctca
     6901 tttctttgca tattccacat ccacgccaaa atgctccata gcttttttgt taagcaaagc
     6961 cgaatgtccg cttccagtat ttagaaccaa tggcttatca ggacatattt catctaaaag
     7021 agtagaatta attgcggggt ttttgtcttc gacccatcct gttgcaagat aaatttcctt
     7081 ctctggatgc ttgtcaataa actcttttat aatggattta tatttttctg cgtcggcggg
     7141 aacaacttga gtaacatttg cctgaccaat tgccctataa ccagcaaaca ttgtatgggc
     7201 atgggcctct aagaatcctg ggtaaataaa tttgtcattg tagttaaaaa cttctgtatt
     7261 gtcatcacaa tactgcatgg catcttcgac agtgccaaca tactgaatct taccatcaac
     7321 aacagtaaca gcttccgcat aaggctgttc ttcattcatt gtgtaaacat tacctatata
     7381 tagttgttga gccttacctt taaaatgcat aataatctgt catcccgcaa ccacagcaaa
     7441 actcaaaacg aggataagac tagacgccaa taagatcttc atcttttttg ataacttttt
     7501 catgctggtt cacctctcaa agacatttaa tacaaattta attatatatt aaaacttaat
     7561 tcataaaacc aaaagtaata gtatgaagcc ccttattatc ttctattttc actgtgaaaa
     7621 tttaccgatt aataaataat atgtaaaaaa ccatattata atactaataa aaacctacta
     7681 tcttattaat ggaggttgcc atgcatattt tggagacttt ggcacaatgc tcacagatgg
     7741 cacacccgaa atcacccccg aagaacagca aaaagcctcc gcaactcaaa tcctaaaatt
     7801 cattaacaat taaatttagc gaccaatcgc aataaaatat taatacttga atgttttata
     7861 atttattata tgggagtaca tcattccaat gcgatcttat aaattcttta taagtacctt
     7921 tggtcgtatc gatcttaagc ataggaatga tgctagacac atatgtagtc ccgtcaatat
     7981 aataatgaca ataaccatca taatagatat catgaaaagt gtcattttta gctatgtccc
     8041 atgttccaat atgaccggct attatatttt tgtctaatcc ctcaactcta cctatatcag
     8101 caggatactt atctgtaaaa taataatcat tagttcccaa ctcccataga tcaccagcct
     8161 cttcatctat gccagcgtga acaaaaatat tattttctat aacatgatat aaaggtaatt
     8221 cgctaaacca atctagaaga acactttcaa atttttcatt aaatgacgaa gattcgtcaa
     8281 ccggcattct accttctaaa caccatcttt cgtgatttcc taacaaagca ataaccttat
     8341 ctgtaccata tttttcttga agacgaataa tagcattaca tacttcgtaa ccgccatttc
     8401 cgccatgaat gtagtcacca agcaaaataa gcttgctatc ttcacatttt aaattatcag
     8461 caataattga aaggttttct ttaaattcat caataaaacc atgtatgtcg ctcatgcaat
     8521 aaataatcat atctattagt gacctgccct ttcaaattgt tgtaaacatg cttattaatt
     8581 atcaaaaaac taaattttat ttagccttta taaaacttta tattgataat cttcattcaa
     8641 tgatctaaac tcggattcag acacatctaa ttcaaaaaca tttactcgtt ctttatagaa
     8701 gtatgcagtt ctgtgattaa tattcttttg atacgggtaa aactcaactt ctagtcataa
     8761 atcattaaag catctacgct catctgaatt tatttgtcta actactccaa atgtattgtc
     8821 gtagatattt tttactacac cacctacctt ataaggaatt ggtattttat taagagcacc
     8881 tgggcggaaa ttttcgacat aatctttttc ataataattt tcccgttcaa tacgtcaatc
     8941 aatataatca ctcaattcgt ttttaatata gacgtaatca gtaaaatttt taacttctat
     9001 gtatgataca tccttgttat caaatatttg aatactctgc gtatcgttat tattgagcat
     9061 ctctttacag ttttcagtct tatataattt tacaaaaata ttttcatact cattattatt
     9121 ttcgctcttc tttaggttca gaaaattctc aaccattgga aaacattcaa acacaagtaa
     9181 cggattacct tgtttatctg caacaacata cctgtattcc tttccattgt tgcaacaaaa
     9241 ggtactataa gcagtattaa tatcttctat gttattctca atctgatttt ttatagttgg
     9301 atgaatatcg ccttttgcaa gttctgttaa tttattaatc ttttgtctat aatctagagc
     9361 tttcgaatta cagattatag aagctttcaa tttatctgag attgtaattc tgtttgacaa
     9421 ataataatct tttaaaaact gtggtatcaa atccataatt aaccctttcc ttatttattt
     9481 ttttctgttt agttttattg actatctaaa tcttttctaa ttaattccca aacaaatttt
     9541 gcggttttat ctggagaata atttttatct ttcatgtcca aaacaggaac ttcctcaata
     9601 tcataacctc atggtttttc agcattttcc ttagatacaa tgcacggata ggaatataag
     9661 aattgatgct taataatata gttatcgcca tccttttttg gcgaaggatt gtaagcacca
     9721 cctatctctg gacttccgta taaatttttt acaatctgaa tttctgtaaa gttatgatga
     9781 gcatttccag taacaacatc gagatcttcc ctaactaagc tgacaatata attattaatt
     9841 aaattatcaa atttatgcac aattgggatt caataataat gcgatttacc attaactcct
     9901 cttcctgtaa acacacaagg atttccattt cttgaacaat taacataaaa cattccaggc
     9961 agattcttta tgcgcccgca acatccgtat gaactttttt taaaataatc atcatcaata
    10021 aactcttgac ttaatttttc aatcaattcc tttttgtttt gttctaatct actctttaat
    10081 aaatttcatg tactattcat tttttcctct ctttccttta ggttaacaac aaaattaagc
    10141 tctatttttt ataaaaccaa tatttccttc agtgttttta atcttattaa gactttcaaa
    10201 atcaacatct aatagtgttt tt
//
LOCUS       DBNCDC010000029        10176 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_2768_length_10176_cov_5.416757, whole genome shotgun sequence.
ACCESSION   DBNCDC010000029 DBNCDC010000000
VERSION     DBNCDC010000029.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 10176)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10176)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10176
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2768_length_10176_cov_5.416757"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="AC04VQ_03325"
     CDS             complement(<1..380)
                     /locus_tag="AC04VQ_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIO9553221.1"
                     /translation="MENKKVKKDNHSNSKLDRKMGLGALIGLGVGATVGSGIFSTISS
                     VADAAQSSLFLVIAFAVATLLQIPGSFCYAELASAYPEDGAHYVYFREAGFKMFTFII
                     GWITFLALDAPALSVMSLAIVNYL"
     gene            complement(451..1668)
                     /locus_tag="AC04VQ_03330"
                     /pseudo
     CDS             complement(451..1668)
                     /locus_tag="AC04VQ_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(1719..2498)
                     /locus_tag="AC04VQ_03335"
     CDS             complement(1719..2498)
                     /locus_tag="AC04VQ_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIO9553222.1"
                     /translation="MAKKKTKFEIKDWYYLAGPLSLVAFFLMDVIGGFNYPGYDWSGQ
                     VIQDLLSLNSRSFPFALVFCIIYFLLTVFAAYCIYMFFKNKKLNKLLSRGIKIFLIGA
                     IVGALSLAVFYQPESGTYAKVKDQGIIAMKDEVVDSESDTQETQEVFDADATMENLAT
                     VGEMVAHPLIIGNIALTFASLVLSIIAFIFMIIGGFKKQGKLFFAAVSIFCLILVLYS
                     IVSFLVMDTSIMGLNARFASYSIVLFMAFLGSYIYITDIKE"
     gene            complement(2607..3725)
                     /gene="ald"
                     /locus_tag="AC04VQ_03340"
     CDS             complement(2607..3725)
                     /gene="ald"
                     /locus_tag="AC04VQ_03340"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIO9553223.1"
                     /translation="MIVGCPKEIMNSENRVGLTPDNVAQYVAHGHKVLVQTKAGEASG
                     FSDAEYRKAGAKIVKTAREVWSKAEMIQKVKEPIESEYKLLKKESIVYTYLHLANHKG
                     LTEAMLKKKVKGVAFETITNPDGTGRPCLWPMSQVAGLLSIQEGAKYLERTYGGEGLL
                     LHGVPGVKKGNVVIIGGGMAGTYAAKAAVGIGANVTIMDVDLNRLAYLEDIFGDRVTT
                     LYSSPGNIENAIKDADIVVGSVLIPGAKTPQLIRKKHLKLMKKGSVIVDIAIDQGGAI
                     ETSHVTTHQDPIFIQDGIVHYCVGNMPGAVPYTSTMALTNATIRNGLLIADKGLEAAC
                     KINPGLVPGINTYDGKCVFKGVADAFRIKYTPIEEVLK"
     gene            complement(3874..4587)
                     /locus_tag="AC04VQ_03345"
                     /pseudo
     CDS             complement(3874..4587)
                     /locus_tag="AC04VQ_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
     gene            complement(4793..7516)
                     /locus_tag="AC04VQ_03350"
                     /pseudo
     CDS             complement(4793..7516)
                     /locus_tag="AC04VQ_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077175.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
     assembly_gap    7517..7616
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7617..8020)
                     /locus_tag="AC04VQ_03355"
     CDS             complement(<7617..8020)
                     /locus_tag="AC04VQ_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein transcriptional repressor
                     HspR"
                     /protein_id="HIO9553224.1"
                     /translation="MDDRDRPVYMISVAAELAGVHPQTLRGYEQKGLVVPKRTEGNTR
                     RYSQADIERLSLISELTEEGINLAGVIRILDLQGRLDERNKEVDDLHSRVRRLKDRVH
                     ELETRESVKELVNDNENSLAIRKHGGLLEEII"
     gene            complement(8020..8703)
                     /locus_tag="AC04VQ_03360"
     CDS             complement(8020..8703)
                     /locus_tag="AC04VQ_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIO9553225.1"
                     /translation="MRSGEGIFGTDWDFKSNGGQGFGRDASGASTFDPFGRAGMGYEQ
                     AVPKESFDIKADLNISFDEAFRGAKKNFSVRIPGKSKADSISLKIPEGCPEGTKLRLK
                     GQGKPSPSGGNGDLICEIHISPHKYFELDGKNVIVKVPVTACEASLGDKVEVPTPDGK
                     KLRIRVPKGTQSGTKLTVKGKGAKKRGGGFGDLIVKIEVVVPKKLTKEQEKKLKAYSE
                     VEDKGVRPW"
     gene            complement(8722..9066)
                     /locus_tag="AC04VQ_03365"
     CDS             complement(8722..9066)
                     /locus_tag="AC04VQ_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HIO9553226.1"
                     /translation="MANNAPDYYKTLGVSKDADDKEIKKAFRKLAQKHHPDAGGDEEK
                     FKEINEAYEVLSDPKKRQVYDQFGTAGAAGFQGGNPFAGGAGGPFGGGATYVNVNGMP
                     FDIGDIFKGAKG"
     gene            complement(9101..9787)
                     /gene="grpE"
                     /locus_tag="AC04VQ_03370"
     CDS             complement(9101..9787)
                     /gene="grpE"
                     /locus_tag="AC04VQ_03370"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIO9553227.1"
                     /translation="MANNKKKSGTEAKASSSAKKKQTAKQTDKKQNDKEWLEEELKDV
                     KTDVKEDADALMKQAAEQLESLQAEVKEWKDNYLRLHAEWDTYRRRTAEQREAERATA
                     SEGLVSDILPVLDDFERSISFAEDNGEKGLLDGVKAVHSKLISMLEKNGTAVIDPEEG
                     EEFDALQHQAVSTVEDKEKFDESIAGVMQKGYKMGNKVIRPAMVSVTTGGKKRPSEEV
                     EKEDDKKSEE"
     gene            complement(9812..>10176)
                     /locus_tag="AC04VQ_03375"
     CDS             complement(9812..>10176)
                     /locus_tag="AC04VQ_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553228.1"
                     /translation="QELGDKVPEDTKKAAQDAAEKTKKALEGDDLEEVKKCMEELQQA
                     GYKIAEIAYSQASAQGAEGAAAGAGASAGEAKENDDGTIEADYEVVDEDADKKDDKKE
                     EKKEDKKDDKKSAKKDSK"
BASE COUNT         2893 a         2402 c         1755 g         3026 t
ORIGIN      
        1 tgaagataat tcacaatcgc taaagacata actgacagcg ccggagcatc gagagcaaga
       61 aatgtaatcc aaccaatgat aaaagtaaac atcttaaacc ctgcttcgcg gaagtaaaca
      121 taatgcgcgc catcttctgg ataagcactg gcaagctcag cgtaacaaaa acttccagga
      181 atttgaagca atgttgcaac agcaaatgct ataaccaaaa acaaacttga ctgcgctgcg
      241 tcagcaacgc ttgagatagt agaaaatatt ccagaaccaa cagtcgcgcc aacacccaaa
      301 ccaataagtg caccaagccc catcttacga tcaagttttg aattactgtg gttgtctttt
      361 ttgacttttt tattttccat ctgttttctt ttgcttcttt tttgatcaaa ttttgtaagc
      421 aacaatacct gtacctcata aaaggacaag tcaacatata ctttctaaag gatactggaa
      481 cataatgcca ataattaaaa gcaaactaat taagcatgaa attattggac aaattaaacc
      541 tccaggacat atgtatgagg gtttgtaact tttctttttt ctcaaaaaga aaattgaacc
      601 aaaagttgca atatttctaa ttaaggtaat taatgttaaa taacctaata gcatttcaag
      661 atccgaaaca aaagtaagga taatagagta aacacaatga attatgatcg cacaataagg
      721 agacttaaat ttaggatgaa tctttccaaa aatactgaaa aaatatccat cttttgccat
      781 tgcataaccc aatcttggct ggtacatcgt agtgcttgat atactcccga caattactaa
      841 aacagccata attgcaacaa ttataccagc agactcacca actacaggta tcctcgtagc
      901 gacagctaca aaaggagctc cattcgaagc aagttcatcg atatttaaaa cccctgtaga
      961 aacaactgaa agtgacgaat acaaaactat aacaactaca gccataacaa taaaagcaat
     1021 tggcatcact ttctttgggt tcttaacttc tccagtcata taacttggcg catacattcc
     1081 atcataagaa aatgttgttg cagctatggc agcaaatacg ctaaatatag taattgaggc
     1141 tgtcccttcg gagcttccaa cataacttgc cgtagaagtt aataaatcag gattaataaa
     1201 aaatatgcca atgccaataa caataatgaa aggaatgatt ttgattatgg tcaatagcga
     1261 ttggataaaa cttccagctt taacagacat gatattggct aacataaaga tcaatattat
     1321 tacaacagca acacaacgca aaataattca attaaatgga agcaaaacag ataaataatt
     1381 aaccaatcca agagccatta tagatatcga aggaggatcg agcgctcaaa aacttgctca
     1441 tccacacaaa aaagcaagaa gtgggttccc tgcttcgcga acataaacat aatagccgcc
     1501 attctcagga taggcacttg aaagctcggc ataacaaaaa tttgcaggaa tttgaagcag
     1561 tccacctact acaaatgcaa caatcaaaac tattccagaa ccagcaatag cagcaacttc
     1621 attcatggta gaaaaaatac ccgtgcctaa agtggtgcct aagcccaagg caatgacaga
     1681 tcaaagacct agatttcgtt caagtttagt ttccaaaatt actctttaat gtctgttata
     1741 tagatataag agcctagaaa tgccataaat aagacaatag aatacgaagc aaatcgagca
     1801 ttgaggccca taatgcttgt gtccataact aaaaatgaaa caattgaata caatacaagt
     1861 atcaagcaaa atattgaaac cgcagcgaag aaaagcttcc cttgcttttt aaatccacca
     1921 ataatcataa atataaaggc aataatagat aggactaacg aagcgaaggt cagtgcaata
     1981 ttaccaatta ttaaaggatg agcaaccatc tctcctaccg ttgccaagtt ttccatggtg
     2041 gcatcagcat caaaaacttc ttgtgtttct tgagtgtcgg attcgctatc tacaacttcg
     2101 tctttcatag caatgatgcc ttgatccttg accttagcgt atgtaccgct ttctggctga
     2161 taaaatactg caagagacaa agcgccaaca attgcaccta tcaaaaatat tttaattccg
     2221 cgtgataata gcttatttag cttcttgttc ttgaaaaaca tataaatgca atatgcagca
     2281 aaaacagtaa gcaaaaaata gataatgcaa aatacaagag caaatggaaa agatcttgaa
     2341 ttaagagata aaagatcctg aataacctgg cctgaccagt cataacctgg ataattaaaa
     2401 ccaccaataa catccattaa gaaaaacgct actaaagata aaggaccagc taaataatac
     2461 caatctttaa tttcaaactt tgttttcttc tttgccatta taaactccca tttatttcaa
     2521 agaagcatta tagcaaagta ggtggcattt tgcgcataaa aaaacggccc agacgaatcc
     2581 gggccgttag ttttatgact taaaatttat ttaagtactt cctcaattgg agtgtattta
     2641 atgcggaatg catctgcaac acccttgaat acgcacttac catcataagt gttgatacca
     2701 ggaacaagac caggattgat cttgcatgca gcctcaagac ccttatcagc aattaacaaa
     2761 ccattgcgga ttgttgcatt tgtaagagcc attgtggaag tataaggtac agcacctggc
     2821 atgttgccaa cacaataatg aacaatgcca tcctgaatga aaattggatc ttggtgagtt
     2881 gtaacatgag aagtctcaat tgcaccacct tgatcaatag cgatatcaac aattacgcta
     2941 cccttcttca tgagtttaag atgctttttg cggataagtt gaggtgtctt cgctcctgga
     3001 ataagaacag aaccaacaac aatgtcagca tctttgatag cattctcaat atttcctgga
     3061 gaagaataca aagttgttac acgatctccg aaaatgtctt caagataagc caatctgtta
     3121 agatcaacat ccatgatcgt aacgtttgcg ccaataccaa cagcagcttt tgcagcataa
     3181 gtgcctgcca taccaccacc gatgataaca acattacctt ttttaacacc aggaacaccg
     3241 tgaaggagca agccttcgcc tccataagtg cgctccaaat atttagcacc ttcctgaatt
     3301 gaaagaagac cagcaacctg tgacattggc cacaagcatg gacggccagt tccatcaggg
     3361 ttagtaattg tctcgaaagc aacacccttt acttttttct taagcattgc ttctgttaaa
     3421 cccttgtgat ttgcaagatg caaataagtg taaacaatag attccttctt gagtaacttg
     3481 tactcagatt cgataggctc cttaaccttt tgaatcattt cagccttcga ccaaacttcg
     3541 cgagctgtct taacaatctt agcaccagct tttctgtact cagcatcact gaagcccgaa
     3601 gcctcgccag cctttgtttg aactaaaacc ttatggccat gagcaacata ctgagcaaca
     3661 ttgtcaggtg ttaagccaac tcgattttca ctgttcataa tttctttagg acaacctaca
     3721 atcattttct tcctcctttt gtagatagaa ttttgtgacg aaataattct agcactcgcc
     3781 ccacttttcc gcaagaacta tttacttaga agtaatggga atgtcgcttg aaattttgcc
     3841 catgtgagga gttgcgtttc aagtcatcag ctttcaattg ccgttgtcat atttaaggac
     3901 ggtaatgcta gtattatctt gcgcaagctg attataatgt tcataaccta agtcaagaat
     3961 actgcacaat gttgcttgat taaataaact gtgagcggca atcaaaacat ttttacctgg
     4021 attgttttgg gcaatctccg ttaaagcttc tttgccacga gcaccaagct cttgcaaact
     4081 ttcgccgtta ggcaccttaa attttcatgg agtttcttgc caacatttat aattttctgg
     4141 ctgttcttct tttaagtctt tcttatatct gtcggcccaa tcgccgaaat ctcactccat
     4201 taatcgcgga tctgttgtga catctagatt atgacgtttg gcaacagcca cccctgtaac
     4261 attagctctt tgaagagggc tggtaattac aacatcaatt tttgcatctt ttaaatattc
     4321 cgcaagagca ttagcttgtt gcatgccatt gtcgtttaat ggaatgtcaa tctggccttg
     4381 cagtctttct tgcacattat attctgtctg accatggcga accaaaataa tgttcgtcgg
     4441 ctcttgctgc tgctgttcaa ttgcagtatt ttggttgcca ctatcattat tgcctacttg
     4501 tgttgcgcta cagccacatg ttattgtcaa acctatagct aaagcaaaaa acaaagaaac
     4561 aattttcttt gaagttaagt ttttcataat gcctccttga cgtgagcaaa ttataacata
     4621 tactaatttt tatataaaat tttcaaaatg aaacaagaaa attttgaaaa taatgcaaaa
     4681 aaaacgactt actggggtag aaaaacctta aaaaagcaaa ttttcttcct aaaaatagac
     4741 ttactggggt ttaattttaa taaatttacc ccagtaagcc caaaaaatgg atttattttg
     4801 aaatttttac atcgtagtcg ccgtttttgt tgatgaagac tttgactttg cacttatcaa
     4861 caagcttgcc ggcaacaact tcgttagcaa ttttatctac aatctcacgc tgaacaagtc
     4921 tcttgagagg acgtgcacca taaacaggat cgtaaccatc aatagcgagg tgctctaaag
     4981 cttcgccgtc aacttcgaga gtaatgtcgc gatcggcgag gcgcttgcga acatcgttga
     5041 gttgaaggtc aacaattggt tcaatgtcag atagcgaaag ctcatcgaag gtaataatct
     5101 catcgatgcg gtttaagaac tctggcttaa atgttgactt caatgcgtcc tgcatcttgt
     5161 tagcaatgtc agccaagttc tttgtggtca tctcttcagg attgttcatt aaatcagcaa
     5221 gcgacttatc ttcgctcttc gaagctttca aatcctttgg cttacctttg ctatccagag
     5281 cagcatcttt acgcttctca ctaatctcgc gaatctggtc gcttccaatg ttagacgtca
     5341 taataataat tgtgttctta aaagacacaa cacggccttg cccatctgtc aggcggccat
     5401 catcaagaac ttgaagcaaa acgttgaaga catctggatg tgccttctca atttcatcaa
     5461 gcaaaataac actataaggc ttgcgacgaa cagcttcggt tagctgacca ccttcgtcat
     5521 aaccaacata ccctggagga gcaccaatca agcgttgaac gctaaacttc tccatgtatt
     5581 cactcatgtc gatgcggacc attgcctttt cgctgtcaaa taaattctcg gccaaagcct
     5641 ttgcaagctc agtcttacca acaccagtcg gtccaagaaa caaaaagctt ccaattggtc
     5701 gctctgggtc cgaaagcccc gagcgcgaac gacgaatcgc acttgccaca ctatttacag
     5761 cacggtcctg accaataaca cgctcatgca aacggtcttc taggttagca agcttctcca
     5821 ggtcaccttg catcattttt gtaacaggaa ttccagtcca aattgaaaca acttcggcga
     5881 cttcttcgga ggttacttcc tcttttaaga tcgcgccttc cttttgcttt ttctcgagca
     5941 atttctcggc atctttcaaa tctttctcaa gctttggaat tagcgaaaaa cgaatctcag
     6001 acgcctcgga caaatttcct tcgcgggttg ctcgctcttc gtcagctcga gccttatcaa
     6061 gttcagtctt gatattttga acgttgacaa taacgtcctt ttcgttttgt cattcagcct
     6121 tcttgctgtt aagcgcatct tgagccgatg caatttcctt ttgcaatttc ttcaaacgct
     6181 ctttagaggc gtcatccttt tccttcataa gcgcctgctc ttcaatttgc atttgggtca
     6241 acttgcgctc agccgcatcg acctcttcag gcatgctatc aatttcaata cgaagacgcg
     6301 aagccgcctc gtccatcaaa tcaattgcct tatcaggtaa aaagcggtct gtaatgtagc
     6361 ggttcgaaag ctcagccgca cttacaattg cgccatcctg gattcgaaca ccatgatgaa
     6421 tttcgtattt ttctttaaga ccacgtaaaa ttgcgatggt gtcttcgacg ctcggctcac
     6481 caattgtaac aggctgaaaa cgacgctcaa gagcagcatc cttttcaaca tactttctgt
     6541 attcatccaa agttgtagcg ccaattgcgt gaagttcacc acgagcaaga gctggcttta
     6601 gcatgttgcc agcatcaagc gaagagtcac ccgtgcttcc agcaccaaca atcgtgtgca
     6661 gctcatcgat gaataaaatg atttgcccag cactatcttt aacttcgcga agaacagcct
     6721 taagtctgtc ctcaaattca ccgcgatact tggcccctgc catcatagca cccaagtcca
     6781 aactaataat gtctttgttt tgaattgacg aaggcacatc ccccgcaaca attcgttgag
     6841 ccaatccttc gacaatcgca gttttacctg ttcctggttc gccaatcaac acagggttgt
     6901 ttttagtcct gcgtgataga acctggattg ttcgacgaat ttcatcggca cgcccaataa
     6961 caggatctaa cttgccttcg cgagcaagtt gagtcaagtt ctggccgtaa agttccaaag
     7021 actcaaattg agctttttcc gtttggtcag taacacgagt atccccgcga agcttgtcgt
     7081 aggcctcctc gatattttta tgcgtgacgc cggcagcatt caagattttg cctgccgcac
     7141 ccttatcctt tgacagtgca ataagcaagt gctcacttgt tgcatattgg tcacctagct
     7201 tttctgcaat tttgactgcc tcatccagca aattcataag ttcagggcct ggcgcaccca
     7261 taggaatcat accgccactg ctttcagttt ttggcatgcc ttcaatttct gcttcaacat
     7321 tttgaagcaa cgtatctggg tccgcaccaa tcctctttat tattgctgag aggttatttt
     7381 cctttgcctc aagcaaagcc ttaagcatat gtactggttg agccatagcg ctcttagctt
     7441 ctccagcaat gttcattgta ttttgtagcg cttcctgcgc ggtaagcgct aattttccta
     7501 agtttcccat attcatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcta
     7621 taatctcttc caataatccg ccgtgcttcc ttattgcaag cgaattctca ttatcgttta
     7681 ccaattcttt aacactttca cgtgtttcaa gttcatgtac gcgatctttc aatctacgta
     7741 ctcggctgtg aaggtcatcg acctctttat tgcgctcgtc taaacggcct tgaaggtcta
     7801 aaattcgaat aaccccggct aaattaatgc cctcttcggt caactcgctt atcagcgaga
     7861 ggcgctctat atccgcctgc gaatacctgc gagtgtttcc ttctgtgcgc tttggcacaa
     7921 ccaatccctt ttgctcataa cctcgcaaag tttgcggatg cacgcccgca agttccgccg
     7981 ctacactaat catatatacc ggtctgtctc gatcatccat taccatggtc tgaccccctt
     8041 gtcctcgact tcgctataag ccttcaattt cttttcttgc tcctttgtaa gtttcttcgg
     8101 cacgacaacc tcaatcttca caatcaaatc tccgaagcca ccgcccctct tttttgcacc
     8161 cttacctttt acagtaagtt ttgtgccact ttgcgtgccc ttcggaacac gaatgcggag
     8221 ctttttgccg tctggagttg gaacttcaac tttgtctccc aagctagctt cgcaagctgt
     8281 aacgggtact tttacaatta cgtttttgcc atctagctcg aaatacttgt gtggcgaaat
     8341 atggatttcg caaatcaagt caccattgcc acctgaaggc gaaggttttc cttgaccttt
     8401 aagacgaagc ttcgtgcctt ctggacagcc ctcaggaatc ttaagtgata ttgaatcagc
     8461 ctttgacttg cctggtatcc ttacactaaa attctttttc gctccgcgaa aagcctcatc
     8521 gaagctaata ttcaaatcag cctttatatc aaagctctct ttaggaacag cttgctcgta
     8581 tcccatacca gctcttccaa aagggtcaaa agtgcttgca cccgaggcat ctcttccgaa
     8641 gccttggcca ccattgcttt taaaatccca atcagtaccg aagattcctt cgccgcttct
     8701 catcctgtct aaaatatcgc ttcagccttt agcgcctttg aagatatctc caatgtcgaa
     8761 aggcatgcca ttaacgttta cataagtggc accgcctcca aatggacctc cggctccgcc
     8821 ggcaaacgga ttaccacctt gaaaacctgc ggcacctgcg gtgccgaact ggtcataaac
     8881 ttgacgcttc ttcgggtcac ttaaaacctc ataagcttcg ttaatttctt taaacttttc
     8941 ctcgtcaccg cctgcatctg gatgatgctt ttgagcaagc tttcgaaaag ccttctttat
     9001 ctctttgtcg tcagcgtcct tagagacgcc gagggtctta taataatctg gtgcattatt
     9061 tgccataata tgcgcctcct ttctaattta gagacgataa ttattcttca gattttttat
     9121 cgtcttcttt ttcgacttct tcgctaggac gcttcttgcc accagttgtc acactaacca
     9181 tagctgggcg aataacctta ttgcccatct tatagccttt ttgcataaca cctgcgatgc
     9241 tctcgtcaaa cttttcttta tcctcaacag tagaaacagc ctgatgctgc aaggcatcga
     9301 attcttcgcc ttcttcagga tcaataacag ctgttccatt tttctctaac atactgataa
     9361 gctttgaatg gactgctttt acaccatcaa gaagtccttt ttcaccatta tcttcagcaa
     9421 agctgatgga gcgttcaaaa tcgtcaagaa ctggtaatat atcgctcacc aatccttcac
     9481 tagcagtcgc tctctcggcc tcacgttgtt cggccgttcg gcgacgataa gtatcccact
     9541 cagcatgtaa gcgaagatag ttatccttcc actcttttac ttcggcctgc aagctttcaa
     9601 gctgctcagc tgcctgtttc ataagagcat cagcatcttc tttaacgtca gttttaacgt
     9661 ctttaagctc ttcttcgagc cattctttgt cgttttgctt tttatcagtc tgctttgcag
     9721 tctgcttctt cttagccgaa gaggaggctt tcgcctccgt ccccgacttc tttttattat
     9781 tagccataat gattcctctc tcttctaaag attatttgga atctttcttg gccgattttt
     9841 tgtcgtcctt tttatcttcc tttttttctt ctttcttgtc gtccttttta tctgcgtctt
     9901 catcaactac ttcgtaatct gcttcaattg tgccatcatc attctcttta gcttctcctg
     9961 ccgaagctcc agcacctgct gcagcacctt cggcaccttg agcgctagcc tggctgtagg
    10021 caatctcagc aatcttataa cctgcttgtt gaagttcttc catgcatttc ttcacttctt
    10081 ctaagtcatc gccttccaaa gccttcttgg ttttctctgc agcatcttga gcagctttct
    10141 ttgtatcctc aggaactttg tcgccaagtt cttgga
//
LOCUS       DBNCDC010000030        10113 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_2807_length_10113_cov_8.698449, whole genome shotgun sequence.
ACCESSION   DBNCDC010000030 DBNCDC010000000
VERSION     DBNCDC010000030.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 10113)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10113)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10113
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2807_length_10113_cov_8.698449"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="AC04VQ_03380"
     CDS             complement(<1..493)
                     /locus_tag="AC04VQ_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553229.1"
                     /translation="MIPTIIKFMAARKKTANTITNILPRIPKMIFVVLLRSIRATIHP
                     TKQDKASAIIEITNVIRTPQISAATENFVVKYIKTAGNQNKVENMTQLKNRTAMRENR
                     IIRGGIGIERSRVLSFALKIMLYETNALNAMQAISAINPNKKKYIQSKPALTKPSTIA
                     HAQR"
     gene            546..1256
                     /locus_tag="AC04VQ_03385"
     CDS             546..1256
                     /locus_tag="AC04VQ_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553230.1"
                     /translation="MAFGKAASGDVASFLIMLAIGLVFFGAIYFIMSKTFMFIVTAKT
                     GGKRKVYKATAQKSKKLSRSLLNKEFSFLFKTPVYLINAGLGSVFILIVAVLCCFGIP
                     MINETLPMLRELSTQPGVEFPFNYLNELIPFGILVVPVFCTAFSTLSTPSISIEGKTL
                     WILKSLPIKPYEVFRAKVNLHAIYCLIPSLIFVLLLGVLCKESPFSILAMVLGIIAWT
                     YLTAYFGMFIGTIRANLV"
     gene            1269..1484
                     /locus_tag="AC04VQ_03390"
     CDS             1269..1484
                     /locus_tag="AC04VQ_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553231.1"
                     /translation="MPIKQSLAVGLSLLFNMVIAVIFIVAYPIMIQVTPVSSQTYIIG
                     FAIICAILCFIFDRWLRSSGCRRFVNL"
     gene            1559..2071
                     /locus_tag="AC04VQ_03395"
     CDS             1559..2071
                     /locus_tag="AC04VQ_03395"
                     /inference="COORDINATES: protein motif:HMM:NF039825.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HIO9553232.1"
                     /translation="MAGLTTSQAAGVAAFMGFLAGLLVILVVVGIIYYVLLVIAWWKL
                     FTKAGEKGWKAIIPFYNYYVQCKLTWAPKFFWIIIVLSVLAGVIGGMVQSMTGTVQVI
                     MSFVTLAAYIAVIVLGIISDYRLAKAYGHGGGYTVGLIFLNFIFMLILGLGKSKYQGN
                     VYLKDQKKKK"
     gene            complement(2132..3364)
                     /locus_tag="AC04VQ_03400"
                     /pseudo
     CDS             complement(2132..3364)
                     /locus_tag="AC04VQ_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012312.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3422..3586)
                     /locus_tag="AC04VQ_03405"
     CDS             complement(3422..3586)
                     /locus_tag="AC04VQ_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553233.1"
                     /translation="MSVKFYRRYLTLMAGTGGLLYGIDLGVIAAALPYIENSGHYTPE
                     EIGLIVGMVL"
     gene            complement(3596..4630)
                     /locus_tag="AC04VQ_03410"
     CDS             complement(3596..4630)
                     /locus_tag="AC04VQ_03410"
                     /EC_number="2.7.-.-"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase enzyme family protein"
                     /protein_id="HIO9553234.1"
                     /translation="MWYNLIMENAIEIATHLGFDATSAKPFGSGHINDTFKVETNSGP
                     VILQKINTDIFKDADGMMDNIKKVTDYLSSIGKTTYKVLAYEKPWRVLSFIDGAHSVD
                     VIEDPAQAFTAGAAFGAFQNDLANFKASDLIDTIPKFHNTLWRLEELDTAAKADKLDR
                     LKNVEAEMSFVDKYRSQAGKVLKLMDEGAIPVRITHNDTKINNVMLDDKTGEAIAVVD
                     LDTVMPGCALYDFGDMVRTATASAAEDETDLSKVYSKIEYFEALVKGYLSQAKFLNEA
                     EIENLVFSGILITFTIGIRFLADYLAGDVYFKTDYDDHNLVRARTQFKMIESIKDQRS
                     EFERIVQENV"
     gene            5034..5432
                     /gene="rpsL"
                     /locus_tag="AC04VQ_03415"
     CDS             5034..5432
                     /gene="rpsL"
                     /locus_tag="AC04VQ_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIO9553235.1"
                     /translation="MERNRKEKLMPTINQLVRKGRITSADKVKAPALKSNPQKRGVCT
                     RVYTTTPKKPNSALRKVCRVRLVNGMEVTAYIPGEGHNLQEHSMVLIRGGRVKDLPGV
                     RYRVIRGCLDCASVDNRKQARSRYGTKKEG"
     gene            5434..5901
                     /gene="rpsG"
                     /locus_tag="AC04VQ_03420"
     CDS             5434..5901
                     /gene="rpsG"
                     /locus_tag="AC04VQ_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006361612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIO9553236.1"
                     /translation="MRRTRATHREVKPDSKYNDVLVTQLINKVLLSGKKSIAENIVYG
                     AFDIVEEQTKSDPLKVFKDAMDNLRPTLEVKPKRVGGATYQVPMEVAPRRATTLAIRW
                     LVDYARERKEHSMQERLAGEIMDATNNLGSAMKKREEVLRMAEANRAFAHYRF"
     gene            5921..>6957
                     /locus_tag="AC04VQ_03425"
     CDS             5921..>6957
                     /locus_tag="AC04VQ_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIO9553237.1"
                     /translation="MKELPLSDTRNIGIMAHIDAGKTTTTERILYYTGKTHKIGEVHE
                     GAATMDWMEQEQERGVTITSAATTCFWNYPGGAENGKPYRFQIIDTPGHVDFTAEVER
                     SLRVLDGAVAVFDAVAGVQPQSETVWRQASRYGVPTIAYINKYDRVGADYFHAIETMI
                     DRLGAPAVAAQVPMGAEENFWGVIDLVTMTAWDFKADDKGMTYPEKLDAIPDEFADLA
                     QKKHDELVEAAADCDDDVAMKFLDNEEITVDELKAAIRKGTIACKLHPVFVGSSYKNK
                     GVQELLDAVVDYLPSPLDVPAIKGETKNGEEERPADPSAPFSALAFKIMTDPYVGKLT
                     YIRVYSGSLDSG"
     assembly_gap    6958..7057
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7058..7750
                     /gene="fusA"
                     /locus_tag="AC04VQ_03430"
     CDS             <7058..7750
                     /gene="fusA"
                     /locus_tag="AC04VQ_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006361611.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIO9553238.1"
                     /translation="FKVEANVGKPQVAYRETATKQVDGAEGKFVRQSGGHGQYGHAII
                     NMYPQEAGKGYEFVNKIVGGTIPKEFIPAVDKGIQESLESGVMAGYPVVDVKVELVDG
                     SFHEVDSSEAAFKVAGSMAIKDALRKGNSVILEPMMAVEVETPEEYMGDVMGNLSSRR
                     GKIEGMGDQGNAKTISAKVPLSEMFGYATDLRSGTQGRASYTMQFDSYEPVPKNVSDE
                     IIAKSGGNKTEE"
     gene            7765..8073
                     /gene="rpsJ"
                     /locus_tag="AC04VQ_03435"
     CDS             7765..8073
                     /gene="rpsJ"
                     /locus_tag="AC04VQ_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006361610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIO9553239.1"
                     /translation="MAKQKIRIRLKGYDHELVDQSTKLIVDTALNTGAKISGPIPLPT
                     ERNMYCVIKSPHVNKDSREQFEMRTHKRLIDILEPTQNTVDSLMRLDLPAGVGIEIKL
                     "
     gene            8086..8751
                     /gene="rplC"
                     /locus_tag="AC04VQ_03440"
     CDS             8086..8751
                     /gene="rplC"
                     /locus_tag="AC04VQ_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIO9553240.1"
                     /translation="MIQTIYGKKVGMTQIYSEDDRVLPVTVLSVEPNVIAQVKTSVKN
                     EKTGKTDGYNAVQLAFGSVRKDNKGKARVNKPTAGHFKKVGIEPTKYLREIRLSDEEI
                     AEVKSGDKITVANFAEVKKVDVTGTSKGKGFAGAMKRHGFGGGPGGHGSHFHRAPGSI
                     GMCATPSRVLKGMRLPGHMGVDTVTTKNLELVRIDEEQNIMLIKGAVPGGKNAILKVK
                     AVK"
     gene            8753..9379
                     /gene="rplD"
                     /locus_tag="AC04VQ_03445"
     CDS             8753..9379
                     /gene="rplD"
                     /locus_tag="AC04VQ_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIO9553241.1"
                     /translation="MANVKVKDITGKDAGSAKLEDSVFGVKPNVAVMHEVVRAQRASW
                     RQGSADTKTRSQVSGGGRKPFRQKGTGRARQGTIRAAQMTGGGVVFGPHPRSYAFKVN
                     SKEVKLSMRSALSAKAADNQLVIAKDFAFKKPATKDAAKFLKALKLNGKRVTLVVDEE
                     DVNTYLSFRNIEGVNCIPSSAMNTYDFVDNKALIITEKALAHIQEVLA"
     gene            9379..9678
                     /gene="rplW"
                     /locus_tag="AC04VQ_03450"
     CDS             9379..9678
                     /gene="rplW"
                     /locus_tag="AC04VQ_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIO9553242.1"
                     /translation="MNIQKDPREVIIRPVISERSYDMMEKGKYTFEVAKDSNKIEIKN
                     AVETIFDVKVLNVNVSTVKPKPKRVRYTPGHTKTWKKAIVKLREGDSIELFGNTQ"
     gene            complement(9787..10062)
                     /locus_tag="AC04VQ_03455"
     CDS             complement(9787..10062)
                     /locus_tag="AC04VQ_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553243.1"
                     /translation="MSFFEDRKLFGIAKKTTPEKLQKRMNFFMAKMSYANHSKNVLYA
                     KTVMEKQAQSIVVEQCKRELRRLIKIANKMDQNDVKFKEVFGSDFNE"
BASE COUNT         2869 a         2004 c         2189 g         2951 t
ORIGIN      
        1 gcctttgagc gtgggcgatg gttgaaggct tggtcagcgc gggcttcgat tgaatgtatt
       61 tcttcttatt tggatttatt gcgcttattg cctgcattgc atttaacgca tttgtttcat
      121 ataacatgat ttttagagcg aaggacaaca ccctacttct ctcgatgcca attcctcctc
      181 gaataattct attttcacgc atggcagttc tattttttaa ttgtgtcata ttttcaactt
      241 tgttttggtt acctgcagtt ttaatgtatt taacaacgaa gttttctgtg gctgcgctaa
      301 tttgtggcgt ccttataaca tttgtaattt cgatgatcgc gcttgctttg tcttgcttgg
      361 ttggatgaat tgttgctcta atactgcgta ataagacaac aaaaatcatt ttaggaattc
      421 ttggaagtat attagtaatt gtgttagccg ttttcttgcg cgctgccatg aactttatga
      481 tggttgggat cattcaaaac gctggagctg tcgatgaatt tatgaaggaa aatttcccat
      541 gagttttggc ttttggtaaa gctgcaagtg gggatgtggc ttcgttttta ataatgcttg
      601 caattggtct tgtcttcttc ggggcaattt attttattat gagtaaaact tttatgttta
      661 ttgtcacagc gaagaccggt ggtaagcgta aggtttataa ggccacagct caaaagtcga
      721 agaagttgag ccgcagcctt ctgaataagg agttttcatt tttgttcaag acgcccgttt
      781 acctgattaa tgctggtttg gggagcgttt ttattcttat tgttgcggtg ctgtgttgct
      841 tcggcattcc gatgatcaac gaaacgctgc cgatgcttcg cgagctgtca actcagcctg
      901 gtgttgagtt tccttttaat tatttaaacg agctcattcc gtttggaatt ttagtcgtgc
      961 ctgttttttg tactgctttt tcgacactta gcactccttc gatttctatt gaaggcaaga
     1021 ccttgtggat tttgaagagc ttgccaatta aaccttacga agtttttagg gcgaaggtta
     1081 acctgcacgc tatttattgc ttgattcctt cattaatttt tgttttgttg ctgggagttt
     1141 tatgtaaaga atcgccattt tcaattcttg ctatggtgct tggaataatt gcttggactt
     1201 atttaactgc gtattttggc atgttcattg gaactatccg tgctaatttg gtttgaataa
     1261 atattacaat gccaattaag caaagtcttg cagttggtct tagcttattg tttaatatgg
     1321 ttatcgcagt aattttcatc gttgcctatc ctatcatgat tcaagttacg ccagtttctt
     1381 ctcaaactta catcattggc ttcgcaatta tttgtgcgat tctttgtttc atatttgaca
     1441 gatggctccg aagttcaggc tgcaggcgat tcgttaattt gtaaaatcca caatatattg
     1501 cgttttcttg gcttttgtag tacaatatgt gcccgtaagt tagagaaagg aactttaaat
     1561 ggcaggctta actacatctc aggctgcggg agttgccgct ttcatgggct ttttagctgg
     1621 tttacttgta attttggtag ttgttggaat tatttattat gtactacttg taattgcatg
     1681 gtggaaatta ttcacaaaag ctggcgaaaa aggctggaaa gcaatcattc cattttacaa
     1741 ttattacgtt caatgcaaac ttacatgggc acctaaattt ttctggatta ttattgttct
     1801 tagtgttctt gcaggcgtaa ttggcggaat ggttcaaagc atgactggca cagtccaagt
     1861 aatcatgagc ttcgttacat tagctgccta tattgcagtt attgttctag gcatcatttc
     1921 tgattacaga cttgctaaag cttatggtca tggtggcggt tacacagtcg gcttgatttt
     1981 cctaaacttc atcttcatgt taattcttgg acttggaaaa tcaaagtatc aaggtaatgt
     2041 ttacctaaaa gatcaaaaga aaaagaaata atctaatata aaaaccaaaa aggtgtaatt
     2101 attgccaggc agtaattaca cctttttttg tttatttttt ctcgaacatc tttgagattt
     2161 cttcgagcgt tttgcctttg gtttctggaa tcacaaatgt ggcgattagg aagtaaacca
     2221 cactaaatac cgccaaaatt ccgaagacga ctgccatgct tgtaacttca actcacaacg
     2281 gaaatatcga agcaattgca gcagacaccg cttgatttaa aatcatggcg atggccattc
     2341 cgtttgcgcg gatctttgtt ggcataagtt cagttaaaac taatcacaca caaacaccag
     2401 gtccaaaagc aaatccagca acaaaaatat agaagaaaat tgaagtgatc acgccagaag
     2461 tcatgccggt tgcaataatg ccagtatcca taagtgccaa cataacagca gcaccaactt
     2521 cgccgattac aattcccgca gttccaattt taagcaaaaa cttgcgaccc tttttatcta
     2581 caagcattac cgcaaaaatt gtgacgaaga aattaatgaa cttgattgca agatcactcc
     2641 agttagcagc ttcgccttga agaccgctgt cacggaaaat tgtaaccgaa taattgagca
     2701 ccgtgttgat tccagtcgct tgattaagca taaggaccag gcaagttagc aaaaacggta
     2761 aaacgtattt cctcttgaga agcgattctt taaccgatgt gctaaaaatt tctttcttgc
     2821 gaacattctt ggcgtcctcg gcttcggcga tttctttgat aattttcttt gcagtttcca
     2881 tgccgttatt tgcagccaaa gaattaagtg cggcatcctt cttgcctttt ttataaagcc
     2941 accttggaga ttctttcaag aaaaacgcgc cgaagaacaa aaacaccgac ggtataacag
     3001 aaacggcaaa aataatttgt catgctgcgg tccaagaatc aagtgttgct gtgtcaattg
     3061 tttgaccttg agcaggtgca tatgcaggtc caacaataga tgttacaatc agtccaacca
     3121 acgcggcgaa gacaaggccc aaggtcagta taagttgaaa cattgcggtg ccggagcctc
     3181 ggcgatcggc atcaagacac tccgccaaat acattggcac tacaactccg ataagaccac
     3241 ctgatgctcc ttgcaaaatt cgaccaaaag cgagaagtgg aaagtttccg cctgggaaga
     3301 acccgcttgc acaacaaaca ggaattgaaa cagtgaagaa aagcgcggaa gcaattatga
     3361 ttttcttgcg accgaatcat tcagcaagtt gaccggcaaa caaggaagac aggaccgtgc
     3421 ctcacaaaac cataccaaca attaagccaa tttcttctgg agtgtaatgt ccagaatttt
     3481 caatatatgg cagtgctgca gcaataactc caaggtcaat gccatacaaa aggccacctg
     3541 taccagccat taaagttaaa taacgtcgat aaaattttac actcattggg gctccttaaa
     3601 cgttttcttg aacgattctt tcaaattcgc tgcgctgatc tttgatagat tcaatcattt
     3661 taaattgtgt tctggcgcga accaaattgt gatcatcata atcagtttta aaataaacat
     3721 cgccggccaa gtaatcggcc aaaaacctga tgccaattgt aaaagtaatc aagatccctg
     3781 aaaatacgag gttctcaatt tcggcctcat tcaaaaactt cgcttgagaa agatagcctt
     3841 taacaagcgc ctcaaaatat tcaatcttcg agtaaacctt actcaaatcc gtttcatctt
     3901 cggccgccga agccgtggcc gtccgcacca tatcaccaaa atcatatagc gcacaacctg
     3961 gcataacagt gtccaaatca acaaccgcaa tagcttcgcc ggttttatca tcaagcatga
     4021 cgttgttaat cttagtgtcg ttgtgagtaa tgcgaaccgg aattgcgcct tcatccataa
     4081 gtttcaaaac cttgccagcc tgactcctat atttatcaac gaagctcatc tcggcttcca
     4141 catttttgag gcggtcaagc ttatcagcct tggcagctgt gtcaagctcc tctaaacgcc
     4201 aaagcgtgtt gtggaacttc ggaattgtat caattaggtc acttgctttg aagtttgcaa
     4261 ggtcattttg aaaggctccg aaggccgctc ccgcagtgaa cgcttgagca gggtcttcga
     4321 taacatccac gctgtgagcc ccatcaataa aactcaaaac tcgccaaggc ttttcataag
     4381 ctaaaacctt gtaagtagtc ttgccaatgc ttgaaaggta gtcggtcacc tttttgatgt
     4441 tgtccatcat gccatcggcg tctttgaaaa tgtccgtgtt aattttttga agaattacag
     4501 gaccactgtt ggtctcaacc ttaaaagtat cgttgatatg ccctgagccg aagggctttg
     4561 cggaagtcgc atcgaatcca agatgtgttg caatttcaat agcattttcc ataatcaaat
     4621 tgtaccacaa tagaggagtt ataaaatgcg taaggaaaat taataacaaa ataatattgt
     4681 gtgtagaatt cgtcctttat gtgttttttt attcaaatga atatatatcg cgtcaataat
     4741 gtatagaaat agtcctttat gtgtttttat aaacaaaatg ttttatttaa taataaaaag
     4801 acctgataaa tatttaagtt aaataaaaaa ataataattt aatgaaaaaa acgcataaag
     4861 gacgcaatcc atacatttgg atataaattt aggggattga cgccggtgag tacgccaggg
     4921 tgggttgtct taaatagttc ttgacaacct gcatcttcac gagtaatatg tgcagtccgc
     4981 gcgatttttg cgagcaggta ttttgttgca ttgctccagg tcacgcaagg aaaatggaaa
     5041 ggaatagaaa ggaaaaatta atgcctacca ttaaccagtt agtaagaaaa ggccgcatta
     5101 cttcggccga caaggttaag gctcctgcgc ttaagagcaa tcctcaaaag cgtggtgtct
     5161 gcaccagggt ttacacaaca acccctaaaa aaccaaactc tgcacttcgc aaggtttgcc
     5221 gtgttcgtct tgtaaatggc atggaagtta cagcttacat cccaggcgaa ggccacaacc
     5281 ttcaagagca cagcatggtt ttaatccgtg gaggtcgtgt aaaggatctt cctggtgttc
     5341 gttatagagt tattcgcgga tgcttagatt gtgcaagcgt tgacaatcgc aaacaggctc
     5401 gcagccgcta cggaacaaag aaggagggct aagatgagac gtactcgtgc aactcacagg
     5461 gaagtaaagc ccgattctaa gtacaatgat gtgcttgtaa ctcagcttat taataaagtt
     5521 ttgctgagcg gtaaaaagtc aattgccgaa aacatcgttt atggagcttt cgacattgtc
     5581 gaagagcaaa caaagagtga tccgctaaag gtcttcaaag atgcgatgga caatcttcgt
     5641 cctacgctcg aagtaaagcc gaagcgtgtt ggtggtgcaa cttatcaggt tcctatggaa
     5701 gttgctcctc gtcgtgctac tactttggca attcgctggc ttgttgatta tgctcgcgag
     5761 cgcaaagaac acagcatgca agagcgcctc gcaggtgaga ttatggatgc aaccaacaat
     5821 ttaggttcag caatgaaaaa gcgcgaagaa gttcttcgca tggcagaggc aaaccgagct
     5881 ttcgctcact acaggttcta ataggtaaaa ggagacttaa atgaaagaat tacctttatc
     5941 agatacaaga aacatcggca tcatggccca catcgatgct ggtaaaacta ctacaaccga
     6001 gcgtattttg tactacaccg gtaaaactca caaaatcggc gaagttcacg aaggcgctgc
     6061 aactatggac tggatggagc aagagcaaga gcgtggtgta acaattactt ctgctgcgac
     6121 aacttgtttt tggaattacc caggtggtgc ggaaaacggt aagccttatc gtttccaaat
     6181 cattgacact ccaggccacg tagacttcac cgccgaagtt gagcgttcgc ttcgcgtttt
     6241 ggacggtgca gttgcagttt tcgatgctgt tgctggtgtt cagcctcagt ctgaaactgt
     6301 ttggcgccaa gcaagccgct atggtgttcc aacaatcgct tacattaata agtatgaccg
     6361 cgttggtgca gattacttcc acgcaattga gactatgatc gaccgccttg gcgcacctgc
     6421 tgttgctgct caagttccaa tgggtgccga agaaaatttc tggggcgtta tcgatcttgt
     6481 aacaatgact gcttgggatt tcaaggctga cgacaagggc atgacttatc cagaaaagtt
     6541 ggatgcaatt cctgatgaat ttgctgactt agctcagaaa aagcatgatg agctcgtcga
     6601 agccgcagct gactgtgatg atgacgttgc tatgaaattc ttggacaacg aagagatcac
     6661 tgttgatgaa cttaaggctg caatccgcaa aggaactatt gcatgtaagt tgcacccagt
     6721 cttcgtcggt agctcttata agaacaaagg tgttcaagaa cttttggatg ctgttgttga
     6781 ttatcttcca agtccgcttg atgttcctgc tattaagggt gaaacaaaga acggcgaaga
     6841 agagcgtccg gctgatccaa gtgctccatt tagtgcactt gcatttaaga ttatgaccga
     6901 cccttatgtt ggtaagttaa cttatatccg tgtttattct ggttcgcttg attctggnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttt aaggttgaag ctaatgttgg
     7081 taaacctcag gttgcttatc gtgaaactgc tactaagcaa gtcgatggcg ccgaaggtaa
     7141 attcgttcgt cagtctggtg gtcatggtca atatggtcat gccattatta atatgtatcc
     7201 acaagaagcc ggcaagggct atgaattcgt taacaaaatt gttggtggta caattccaaa
     7261 agaatttatc ccggcagttg ataagggaat tcaagaaagc ctcgaatccg gtgttatggc
     7321 aggttatcct gttgtggatg ttaaggttga gcttgttgat ggttcattcc acgaagttga
     7381 ctcttccgaa gccgcattta aggttgccgg ttcaatggca attaaagatg cacttcgcaa
     7441 gggaaattcg gtaattttag agccaatgat ggccgtcgaa gttgaaaccc ctgaagaata
     7501 catgggtgat gttatgggta acttgagttc tcgccgtggc aagattgaag gcatgggaga
     7561 ccaaggtaat gccaagacaa tttcggcaaa agttccactt tcagaaatgt tcggatatgc
     7621 gacagatctt cgaagcggaa cacaaggccg tgcaagctac acaatgcaat ttgattctta
     7681 tgagccagtt cctaaaaatg ttagcgatga aattatcgct aagtctggcg gaaataagac
     7741 agaagaatag aaaggcagat taagatggct aagcaaaaga ttagaattcg tttaaagggt
     7801 tatgatcacg agcttgttga tcagtcaact aagttgattg ttgacactgc gctaaatact
     7861 ggtgcgaaga tctctggtcc aatcccgctt ccaacagagc gcaatatgta ttgcgttatc
     7921 aaaagcccgc atgttaacaa ggattctcgc gagcaatttg aaatgcgcac tcacaagcgt
     7981 ttaatcgaca ttttggaacc aacccaaaat actgttgatt cgcttatgcg ccttgatttg
     8041 cctgctggag ttggcatcga gattaaactt taaggtaggt gcattatgat tcagactatt
     8101 tatggaaaaa aggttggcat gactcagatt tattctgagg acgaccgcgt tttgcctgta
     8161 actgtgctaa gcgttgaacc taacgtaatt gctcaagtta agacaagcgt taagaacgaa
     8221 aaaaccggca aaactgatgg ctataatgct gttcagttgg ctttcggctc tgttcgcaag
     8281 gacaacaaag gtaaggctcg tgtaaacaag cctactgctg gccacttcaa aaaggttggc
     8341 attgaaccta cgaagtatct tcgcgagatt cgtcttagcg acgaagaaat tgccgaagtt
     8401 aagtctggtg acaagattac ggttgccaat tttgccgagg ttaaaaaagt tgatgtaact
     8461 ggaacttcga agggtaaggg cttcgctggt gctatgaagc gtcatggttt tggcggtggt
     8521 cctggtggac atggttctca ctttcatcgt gctccaggtt ctattggtat gtgtgcaaca
     8581 ccttcgcgtg ttttgaaagg catgcgtctt ccaggtcata tgggtgttga tactgttaca
     8641 accaaaaacc ttgagcttgt tcgaatcgac gaagaacaaa acatcatgct tattaaaggt
     8701 gccgttcctg gtggcaaaaa cgcaatctta aaagtcaagg cggtgaagta atatggcaaa
     8761 cgttaaagta aaagatatca ctggaaagga tgcgggcagc gcaaagctcg aagattctgt
     8821 cttcggagtt aagccaaacg ttgctgttat gcatgaagtt gttcgtgctc aacgcgcaag
     8881 ttggcgtcaa ggtagcgctg atacaaagac tcgcagccaa gttagtggtg gtggccgcaa
     8941 gccgttccgt caaaagggaa ctggtcgtgc tcgtcagggt acaattcgtg ccgctcaaat
     9001 gacaggcggt ggcgtggtct tcggacctca tccaagaagc tatgctttca aggttaattc
     9061 taaggaagtt aaacttagca tgcgtagtgc tctttcggca aaagcagctg acaaccagct
     9121 tgtaattgct aaggattttg ctttcaaaaa gcctgccaca aaagacgctg caaaattcct
     9181 taaggcgttg aagcttaatg gcaagcgtgt tacattagta gtcgacgaag aagacgttaa
     9241 cacctacctt tcatttagaa acattgaggg agttaactgc attccatctt cggctatgaa
     9301 cacttacgat tttgttgaca acaaagcttt gatcattact gaaaaagctt tagctcatat
     9361 tcaggaggtg ttggcataat gaatattcaa aaggatccac gcgaagtaat tattcgtcct
     9421 gttatctccg aacgttctta tgacatgatg gagaagggca aatacacctt cgaagttgct
     9481 aaggacagca acaaaattga gattaaaaat gctgttgaga caatctttga cgtaaaagtt
     9541 ctaaacgtaa atgttagtac tgtaaagcca aagccaaagc gtgttcgcta tactccgggt
     9601 cacaccaaaa cttggaagaa agcaattgtt aaacttcgcg aaggcgacag catcgaactc
     9661 ttcggtaaca ctcaataatt taaaggctcc atcacggagc cttttttata gttctggggg
     9721 agttacatct aaagaacgca tagttccagg cgggtttgcc attcatcgca tctccgcagc
     9781 attgtatcat tcattaaaat cgcttccaaa aacttccttg aacttgacgt cattttggtc
     9841 catcttgttt gcaattttaa ttaatctacg aagctcacgc ttgcattgtt caacaacaat
     9901 cgattgcgct tgtttttcca ttacggtttt ggcgtataaa acattcttgc tgtgattggc
     9961 gtaactcatt ttcgccataa agaaattcat gcgcttttga agcttttcgg gcgttgtttt
    10021 tttcgcaatt ccaaacaatt ttctgtcctc aaaaaatgac atttcgcccc cttaaattat
    10081 gcgagtatta tacagtattt attggatttc ccg
//
LOCUS       DBNCDC010000031         9954 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_2877_length_9954_cov_7.543590, whole genome shotgun sequence.
ACCESSION   DBNCDC010000031 DBNCDC010000000
VERSION     DBNCDC010000031.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 9954)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9954)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9954
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2877_length_9954_cov_7.543590"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            177..251
                     /locus_tag="AC04VQ_03460"
     tRNA            177..251
                     /locus_tag="AC04VQ_03460"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:209..211,aa:Val,seq:gac)
     gene            complement(394..672)
                     /locus_tag="AC04VQ_03465"
     CDS             complement(394..672)
                     /locus_tag="AC04VQ_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553244.1"
                     /translation="MSAYSKDPSTAENQIKQIVEDMQSESAKGNYCICGADFNKQLVN
                     NPENYYPKEKVYVMKNFPLEFLDGTNIKVVAPFNPNNPVGSCRSAGQV"
     gene            complement(685..1056)
                     /locus_tag="AC04VQ_03470"
     CDS             complement(685..1056)
                     /locus_tag="AC04VQ_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553245.1"
                     /translation="MRDTADSIANLLNEENADFVSIQEADSDSTRTYHVDETKILRQN
                     MPKYYNVSACCFDSPFLFWPILQPHGESTSNLMTLSKYLPVNNAIRRQFPVDNSFTKI
                     LDYDRCYSVVRYPTSNGKELV"
     gene            complement(1180..1359)
                     /locus_tag="AC04VQ_03475"
     CDS             complement(1180..1359)
                     /locus_tag="AC04VQ_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553246.1"
                     /translation="MTKINKSIFKTLGKAFKLILVTIVVFFTVNIVYLIFEYKRIPDN
                     QELDINLAKDFNPET"
     gene            complement(1372..1872)
                     /locus_tag="AC04VQ_03480"
                     /pseudo
     CDS             complement(1372..1872)
                     /locus_tag="AC04VQ_03480"
                     /inference="COORDINATES: protein motif:HMM:NF039905.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
     gene            complement(1903..2301)
                     /locus_tag="AC04VQ_03485"
     CDS             complement(1903..2301)
                     /locus_tag="AC04VQ_03485"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012981.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIO9553247.1"
                     /translation="MLYHKDMKLLIINNLNSGYGEGSVYDFMRAFARENDELVVRNVI
                     HTTDMIRHLRDADQFDMVVAAGDDDTISKISFFLADTQIPILPYPSGTSNLIAQNLFL
                     PSEPHALAKLARECKTLNFDVGELTVDGKK"
     gene            2383..2904
                     /locus_tag="AC04VQ_03490"
                     /pseudo
     CDS             2383..2904
                     /locus_tag="AC04VQ_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013755.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
     gene            3085..3549
                     /locus_tag="AC04VQ_03495"
     CDS             3085..3549
                     /locus_tag="AC04VQ_03495"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HIO9553248.1"
                     /translation="MISDEQIIQICKQQPKKVDDLFAMRGAKGSLNIESARGIIEAVK
                     RGNAYTEDELPSLRDKHKYSYKSEEFDKNEIEAEIDLMSALVRHRAKEKNIAPQTLTS
                     NSDLTKIAYGVKSGIPTLSGWRKKFIGDELIDLVSGKISLRLDNGNIVLEKK"
     gene            3549..3680
                     /locus_tag="AC04VQ_03500"
     CDS             3549..3680
                     /locus_tag="AC04VQ_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553249.1"
                     /translation="MADEVATPDHYSVSDAVALLKKGLESINIRIIGEVTNLQSTAG"
     gene            3834..4772
                     /gene="xseA"
                     /locus_tag="AC04VQ_03505"
                     /pseudo
     CDS             3834..4772
                     /gene="xseA"
                     /locus_tag="AC04VQ_03505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
     gene            4788..5042
                     /gene="xseB"
                     /locus_tag="AC04VQ_03510"
     CDS             4788..5042
                     /gene="xseB"
                     /locus_tag="AC04VQ_03510"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013979714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIO9553250.1"
                     /translation="MANETKNIKDMTFREAMDELNKTVTLLEGDTLELEESLEKYKFG
                     VELLAHLKGELNNAQVQVEELMGKIEAEDMSDEKRDTTLS"
     gene            5055..6101
                     /gene="trpS"
                     /locus_tag="AC04VQ_03515"
     CDS             5055..6101
                     /gene="trpS"
                     /locus_tag="AC04VQ_03515"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIO9553251.1"
                     /translation="MSEKSFETNLAKSNEVAAEIDKNPGKFRMLTGDRPTGYLHIGHY
                     FGSIMNRVELQNKGVETIVLIADYQVITDRDSTENIQEYVYNMLADYLACGIDPKKTK
                     IFAHSAVPALNQLMLPFLSCVSEAELLRNPTVKTEMKASGHELTGLLLTYPVHQACDI
                     LFCKADIVPVGKDQMPHIEQTRLIARRFNNRYGKVFKEPVGILSKAIEVPGLDGRKMS
                     KSYNNAISLRLSADETAKLIKKSQTDSDRNITFDPKKRKGVSALLTTAGLCSGKDPQD
                     IAAEIGDGGGGALKAYTTEAVNSFLAPIREKREKFLADKAQLQTILEEGNAFANEIAN
                     ETLNEVRKTMGMTY"
     gene            complement(6157..6411)
                     /locus_tag="AC04VQ_03520"
                     /pseudo
     CDS             complement(6157..6411)
                     /locus_tag="AC04VQ_03520"
                     /inference="COORDINATES: protein motif:HMM:NF013073.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            complement(6436..7116)
                     /locus_tag="AC04VQ_03525"
     CDS             complement(6436..7116)
                     /locus_tag="AC04VQ_03525"
                     /inference="COORDINATES: protein motif:HMM:NF048395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HIO9553252.1"
                     /translation="MNEFNNSVKQNKNVGFVVAVVSIVTLFLCLSLSVALSPKKAIAD
                     PDNLQATLDETSNAYFGALAAQEEAQAKVNEAEATIAECEKKIPIVQGKLCTRAKSMY
                     MNRGFGEILELLTGATSIEDLVNNLQYISIVNSKDAALVQSSKDLKAQVESEKASLDA
                     NLAAATEQANLAEAAYNTAQAALAEFKSRQEQGGGGGGGGYTPTQPAEYLGDVVATAR
                     ACLGYPYV"
     gene            7326..8420
                     /gene="ftsZ"
                     /locus_tag="AC04VQ_03530"
     CDS             7326..8420
                     /gene="ftsZ"
                     /locus_tag="AC04VQ_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012798040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIO9553253.1"
                     /translation="MPNLKGQERLAVIKVVGVGGGGTNAINRMVEANVRGVEFIAVNT
                     DRQALYMSNADKVIHIGEELTRGLGAGANPEIGCQAAEESRAEIRDALAEADMVFITA
                     GEGGGTGTGAAPIVAEIAREEIGALTVGIVTKPFAFEGRLRKNQAEQGIDLLAQKVDT
                     LIVIPNDRLLEIVEKKTSMVDAFRIADDTLRQGIQGVTDLITIPGLINLDFADIRTVM
                     KDAGTAMMGIGIASGENRAMEAAQQATNSSLLETSISGATRVLFSIAGGPDLTLTEVD
                     EAAKTVESCADENANIIYGQIIDENLDDSVRITVIATGFTGTPRRTQTVINSGNEQVD
                     PYKAPALNAQEPESKVKDEEYIPDFLKRDE"
     gene            8433..9116
                     /locus_tag="AC04VQ_03535"
     CDS             8433..9116
                     /locus_tag="AC04VQ_03535"
                     /inference="COORDINATES: protein motif:HMM:NF016363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HIO9553254.1"
                     /translation="MPQDFYQEDNNLPADGIGTEIDPRDTYGSRFEVKSREVGSSKPR
                     SAIIAGQKRLRNSDKIFSKQQKDNRGGNVNMKNVNTQDKKVEDNEIRSSGLDSLFSST
                     SESKSKENKFKSEVKTEKASAKKEIPSAQVQNRRLKVIKPISYNSAASEISAALKGGC
                     AVVLYLKGTNEDTSKRLLDFTFGAASMCGAHVSLDAEKTYVITIGPGLSQREKLLCIK
                     EGVALKVEN"
     gene            9185..9919
                     /locus_tag="AC04VQ_03540"
     CDS             9185..9919
                     /locus_tag="AC04VQ_03540"
                     /inference="COORDINATES: protein motif:HMM:NF016959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009987 - cellular process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HIO9553255.1"
                     /translation="MAITSSDIDNQSFSVERKGYSIEEVDIFLETVSKEVGILNDKVE
                     ELEKGASESSKDGASNEEVVQLNATIAKQSAEIEKLKKEVAEKVNDGKAISEALIVAQ
                     RQAEKIVAEANDKAKKIIKDANDKADYIEKDADAQKKKVMQEIDKLEKEQQSCRKEYQ
                     VMLRDIITAMDKRLDESEGLSAKKSPASTNATKAVSGSTSKSAPVKSQASASKTSESK
                     PVQVNPEPEKDFSGFGDTDFDDDKID"
BASE COUNT         3097 a         1842 c         2116 g         2899 t
ORIGIN      
        1 cggacgacgg ggttcgaacc cgcgacccca accttggcaa ggttgtgctc taccaactga
       61 gccacgtccg cgtgagagca tatgatacca aagttgctta taattgcaag aattattttc
      121 actaaaataa tatttatgtt ttaaatgttt gtgttaaaat aggcactcgc atttgtgggc
      181 gattagctca gggggagagc gcttgctcga cacgcaagag gtcgttggtt cgagaccatc
      241 atcgcccacc actctctaaa ttaatctaaa agtcatgtaa actgggttgt ggtctgagta
      301 ttggaagtct gcatcaatta catcgcttgt taacacttga acatttgatg aaaccaagaa
      361 tccatcaatg ttacaaacag gatctttttc gcttcatact tgtcctgcac tacggcaact
      421 tcctacagga ttatttggat taaaaggcgc tacaacttta atatttgttc catctaaaaa
      481 ttcaagagga aagtttttca ttacgtaaac tttttctttt gggtaatagt tttcaggatt
      541 gttcacaagc tgcttgttga aatctgcgcc gcaaatacaa tagttgccct tggcggattc
      601 gctttgcatg tcttcgacga tttgtttgat ttgattttcg gcagtgcttg ggtccttcga
      661 ataagcagac atatgagtgg tgtatcatac aagttctttg ccgttagaag ttgggtaacg
      721 gacaacagaa tagcatctat cgtaatccaa aatttttgta aaactgttat caaccgggaa
      781 ctgacgtcta attgcgttgt ttactggcaa atatttgctg agtgtcatta ggtttgaggt
      841 tgattcgcca tgaggttgta agattggcca aaacaaaaat gggctatcaa aacaacatgc
      901 actaacgttg taatatttag gcatattttg acgaagtatt tttgtctcat ccacatgata
      961 agttcttgta gagtctgaat ccgcttcttg aattgagaca aaatcggcat tttcttcgtt
     1021 tagcaagtta gcaatgctgt ctgctgtatc acgcacagat tgttcagact tcgctcaact
     1081 ttgggttcct ccatccatga agaaagtaaa gtctgggagg taagcgcaaa agccaatgtt
     1141 atacgaaatt gccttataag tatttaatgt atcaactatt caagtttcag gattaaaatc
     1201 cttcgcaaga ttaatatcaa gttcttgatt gtcggggatt cgcttgtatt caaaaattag
     1261 ataaacgata ttaactgtga aaaatacaac aatcgttaca agaattaact taaaggcttt
     1321 gccaagcgtt ttaaaaatgc ttttatttat tttagtcatt gggggctcca attacatttc
     1381 cgaataattc tttaaacatt catcgtcaac gacgtatttt acaccatgtt ttaacacttt
     1441 tgcttcaaat ggtgtattag cgcttatcgg ttttccgtca atttgaattt ccatttcagg
     1501 gtctgccttt acttttacat aacttgatat gtgaatttcg agcgcatcgc ttcgatctgg
     1561 aaattcgacc gctctgtcaa gtgctgcagc tgtgagggcg ggaacatatt taagagctgt
     1621 tttgcctttt aaaattacaa cttcgaattt gccatctcgc ggcctgttgt tatgggcaac
     1681 ggagaggtca aggccaatct tagcgaagtt gagtagcatg acaccaacac cttcggtctt
     1741 tatagttcta tctttaagtt caatttcaaa ttttgaaagt tgaggtttaa agtttgtaag
     1801 cgccgctcca atgtaagcga atgggccgag cgactttcga gttgccatgg cttccttgct
     1861 aatttttgtt ccataaccag ctccagcatt acatccgaag cctcattttt taccatctac
     1921 agtaagttca cctacatcaa agtttaaagt tttacattca cgagcaagtt ttgcaagtgc
     1981 gtgaggttcg cttggtaaga acaaattttg tgcaattaaa tttgaagttc cagaagggta
     2041 gggcaaaata ggaatttgag tatcagctaa aaagaaactt atctttgaaa ttgtgtcatc
     2101 atcgccagcg gcaactacca tatcaaattg gtctgcatca cgaagatgcc taatcatgtc
     2161 cgttgtatga attacatttc gaacaacaag ttcatcgttt tcccttgcaa acgctctcat
     2221 gaaatcgtag actgagcctt cgccgtatcc agaatttaga ttgtttatta tgagtaattt
     2281 catatcttta tgatacaaca tctgcaaact attttgccga aaataagaat agcaaaatgc
     2341 aagttgtgtt atgattagtc ttcgttgaaa gggaactatt ctatgtatat tacagaccag
     2401 caagaatttg aaaacttcct tgaaatagct aataagcagg aggttgttgc gattgatacg
     2461 gaatttttgc gcgacaacac ttattggcca aagctttgtc ttttgcaaat ggccataaac
     2521 ggtaaaactg ctttaattga taccttagaa attgatatta aaccgataaa aaagttactc
     2581 aataataaaa atgttgtaaa gctgtttcat tcgccgcgtc aagatatcga aattttgctt
     2641 cacgaagttg gcgtggttcc tgagccagtt tttgatactc aaattgcagc tggatttttg
     2701 gggctcaaca atcaaattgg ttatggaaat ttggtttctg caattttgaa tattaaatta
     2761 aagaagggtg atacttacac tgattgaagt attcgtccgc taactaactc tcaattaagt
     2821 tacgcaaaag acgatgttgt ttatctgctt gaactgtatt atgtgatggt taaaaagttg
     2881 aaagagctta atcgtcttga ttgagtttat gaagatatta aagttaagta tttgggcaaa
     2941 gattcttatg aagtttcttt ggacgatagg ttttggcatc ttaagcgagt ttcaacatta
     3001 actcgaagac aacttgcttg tgcgagggaa ttggcttctt gacgtgaaaa agctgcaatc
     3061 aagcgaaaca ttccgcgccg ttgagtgatt tcggatgagc aaattattca aatttgtaag
     3121 caacagccaa aaaaggttga cgacctattt gctatgcgcg gggcgaaggg aagcttaaat
     3181 attgaatctg ctcgtggaat tattgaggct gtgaaaaggg gaaacgctta caccgaagac
     3241 gagcttccaa gtttacgcga caagcataag tattcttata aaagcgaaga atttgacaaa
     3301 aacgaaattg aagctgaaat tgatttgatg agtgctcttg ttaggcatcg tgctaaagaa
     3361 aaaaatattg ctccgcaaac tcttacctct aattccgacc taaccaaaat tgcatatggt
     3421 gttaaaagcg gaattccaac gctttcaggg tggaggaaaa agtttatagg ggacgaattg
     3481 attgatcttg tcagcggaaa aatttcgctt cgtttagata atggcaacat tgttctggag
     3541 aagaaataat ggcggatgaa gttgcaacac ccgatcatta ttcagtcagc gatgcagtag
     3601 cactgcttaa gaagggtctt gaatctataa atattagaat aataggtgaa gttacaaact
     3661 tacagtctac agctggctga aaatctatat tttttagtgt gaaagacgaa aaagcacaga
     3721 ttccttgtca aatatgaaaa tcaaaacatg aaccattagg tattgaacta caagatggtt
     3781 ctctagttga attgtctgga agatttagtg tttgaccagt aaagggagag acgaagtttt
     3841 attgtgattc tattacgctt gccggcgaag gtgagcttcg tatgcaagtt gcgcgccttg
     3901 caaaaaaact tgaagctgaa ggtttgacag atccttccaa aaaattgcca attccaacca
     3961 tgccaaacaa gattggtttg gtcacatctc ctcgaggagc tgttgttcac gatgtaattc
     4021 gaacgcttag acgtcgcatg ccttttgcaa atgttgtttt tgctggcgtt ggagttgagg
     4081 gagcgaatgc tccggcagat atgataaaag cgctggatac tgtgtcttcg gcaggagtgg
     4141 atgtaataat tttatgccga ggtggcgggt ccttcgaaga tttgatgcct tttaacgacg
     4201 aagctctcgc gctcaagatc gccgaattga aaacgccaat tgtaactgga attggacacg
     4261 agccagacac gacaattgca gatttagtta gcgactttcg cgcgagcact ccaacaggtg
     4321 cagccgaagc cgttagcata aacgccgaag aactgattgc gaacttttct gcccacattg
     4381 aaaaggctaa gacttcaatt attggaagag ttaacgcaac ggcgcagatg ctcaaccaaa
     4441 ttgcttcgcg tcctatattt gctgatggga caaagctttt tgaggctgaa tttcaaactc
     4501 ttgattacaa tcaagaaaaa ttgcaaagaa tgttgggtga actttcaaaa aaatacgaca
     4561 atcaaatttc gcttgctgcg gctaaacttc aagataaatc tccgcttgtt attttgtctc
     4621 gtggttatgc tgcggcgaaa acttcggaag gcaaaattat taaaaacatt tcacaggtgt
     4681 ccgaagggga cacgatgagc gttcgcgtgt ccgatggcgt aatagacaca aatgttgaaa
     4741 ggacgcaaaa acttgatgga gattctatat aataggtggg agtaattatg gctaatgaaa
     4801 ctaaaaatat aaaagatatg acatttcgcg aggcgatgga cgagcttaac aaaaccgtta
     4861 ctcttttgga aggggatacg ctagagcttg aagagagcct cgaaaaatac aaatttggag
     4921 ttgagcttct tgctcattta aagggtgagc ttaataacgc ccaggttcaa gttgaggagc
     4981 ttatgggcaa aattgaagcg gaagatatga gcgatgagaa gcgtgataca acgctgtctt
     5041 aaaaggattg aattatgtca gagaaaagtt ttgaaacaaa tttagcaaag agcaacgaag
     5101 tagcagctga gattgataaa aatcctggca aattccgcat gcttacaggc gaccgaccta
     5161 ctggctatct ccacatcggc cattacttcg gaagtattat gaatagagtt gagcttcaaa
     5221 acaagggtgt ggaaactatt gttttaattg ctgattatca agtcattacg gatcgcgaca
     5281 gtaccgaaaa cattcaagaa tatgtttaca atatgctggc cgattatttg gcttgcggca
     5341 ttgatcctaa aaagacgaag atcttcgccc atagtgctgt tcctgctcta aatcaactta
     5401 tgcttccgtt tttaagttgc gttagcgaag ccgaattgct tcgtaacccg accgttaaaa
     5461 cagaaatgaa ggcttctggg catgagctta ctggtcttct tttgacatat cctgttcatc
     5521 aagcttgtga tattttgttt tgcaaggctg atattgtccc tgttggaaaa gatcaaatgc
     5581 ctcatatcga gcaaacgcgc ttgattgctc gtcgctttaa taatagatat ggcaaagttt
     5641 ttaaagagcc tgttggaatt ctttcgaagg ctatcgaagt cccaggtctt gatggcagaa
     5701 aaatgtcgaa gtcatacaac aacgcgattt cgcttcgtct ttctgccgat gaaactgcca
     5761 agcttataaa gaagagtcaa actgacagtg atagaaatat tacttttgat ccgaagaagc
     5821 gcaaaggtgt atctgcactt ttgacaactg ctggactttg cagtggtaag gaccctcagg
     5881 atattgctgc tgaaattggc gatggtggag gtggagcgct taaggcttat accaccgaag
     5941 ctgtaaattc cttcttggcg ccaattcgtg aaaagcgcga aaagtttctt gctgataaag
     6001 ctcagttgca aactattctt gaagagggta atgccttcgc caatgaaatt gcaaatgaga
     6061 ctttaaacga agttcgcaaa actatgggaa tgacatatta aaaaaagagg cgcccgaatt
     6121 ctcaagcgcc tcttaattgc aaaatcttaa aatagcttaa cgtcttgtga aatattccat
     6181 tccgccttga acgccagctt ccttaactac gtcaccttct gttggtgcat gaatcatcat
     6241 tccgcctcca atatagattc cgcagtgaga ccacgtggaa acaacatcgc ctggtactgc
     6301 ttgcgaagca tcaattctgt ggaaacctga actctcagtc ataaatgtat ctgtaataaa
     6361 catatggctt ccgccaccgc cgtagcacca acttactaat ccactacaat caaaaccata
     6421 aggacccgca gcagctcata catatgggta gccaagacaa gcgcgagcag tagcaacaac
     6481 atcacctaaa tattctgctg gttgtgtagg tgtgtatcca ccacctccac cgccaccgcc
     6541 ttgttcttgt cgtgatttaa attcggcaag agcagcttgt gctgtgttgt aagcagcttc
     6601 agcaaggtta gcttgctctg tagctgcagc aagatttgca tccagtgaag ctttttcgct
     6661 ttcaacttga gctttaaggt ctttgctact ttgaaccaaa gcagcatctt tgctatttac
     6721 aatactaatg tattgcagat tgttaaccaa gtcttcaatt gaagttgcgc ctgttaaaag
     6781 ctcaagaatt tctccaaagc ctcggttcat atacatactc tttgctcgag tacataattt
     6841 accttgaaca atagggatct ttttttcgca ttctgcgatg gtagcttcag cttcgttgac
     6901 cttagcttgt gcttcttcct gcgctgctaa tgctccaaaa taagcattag aagtttcatc
     6961 aagtgtagct tgcaagttgt ccggatctgc tattgctttc ttcggactta aagcaacact
     7021 taaagataag cacaaaaata aagtcacaat agaaactact gcgacaacaa aaccaacatt
     7081 tttgttttgc tttacgctat tattgaattc attcatataa caacctcctt taaatttctt
     7141 tttagcgctt ttggcgccgt gtctccgtat acgaattaat gcaactcgca taaagcatcg
     7201 tatattttac cactaatcct aaaaaaatcc aaaaaaagtt atttttttct tgcacacgca
     7261 cttaatatgt tgtagtatac gcatctgtgt aggaatcatt tattttttta aaaatggagg
     7321 atattatgcc taatctaaaa ggtcaagaaa gacttgcagt aatcaaagtt gttggcgttg
     7381 gtggaggcgg tacaaatgca ataaaccgca tggtagaggc caatgttcgc ggtgttgaat
     7441 tcattgctgt aaacacagat cgacaagctt tatatatgtc aaatgctgac aaagttattc
     7501 atatcggcga agaattaact cgcggtttgg gtgctggtgc taatccagaa atcggctgtc
     7561 aagctgcgga agagagccgt gcagagattc gcgatgctct agctgaagct gatatggtat
     7621 ttattacagc tggcgaaggt ggcggaacag gcactggagc tgcacctatt gttgcagaaa
     7681 ttgcacgcga agaaattggt gctttaactg ttggtattgt tacaaagcca tttgccttcg
     7741 aaggacgtct tcgtaaaaac caagctgaac agggcataga ccttcttgca caaaaagttg
     7801 atacattaat tgtaattcca aatgaccgtt tgctagagat tgtagagaaa aagacaagca
     7861 tggtagacgc atttagaatt gctgatgata cacttcgtca aggtatccag ggtgttactg
     7921 atcttattac aattcctggt cttattaacc ttgactttgc tgacattcgc actgttatga
     7981 aggatgctgg tactgcaatg atgggtattg gtattgcttc gggcgaaaat agagctatgg
     8041 aagctgctca acaagctaca aattcaagcc ttttggaaac ctcaatcagt ggtgctacac
     8101 gtgttctttt ctccattgcc ggtggcccag atcttacact tacagaggtt gacgaagcag
     8161 ctaagacagt tgaatcttgt gccgatgaaa acgccaacat catctatggt caaattattg
     8221 acgaaaactt agatgatagc gttcgcatta ctgttattgc aacaggtttt acaggcactc
     8281 caagaagaac ccaaactgtt attaattctg gaaatgagca agttgatcct tacaaagcac
     8341 ctgcacttaa tgctcaagag ccagaatcga aagtaaaaga cgaagaatat attccagatt
     8401 ttctaaagcg cgacgaataa tatagaggag ccatgccgca ggatttttat caagaagata
     8461 ataatttacc tgcagacgga atcggaactg aaatcgatcc gcgagatacc tatggctctc
     8521 gtttcgaagt aaaatcaagg gaagttggta gctcaaagcc aaggtcagct attatagctg
     8581 ggcaaaagcg tcttcgtaat tctgacaaga tattttcaaa acaacaaaaa gataatcgtg
     8641 gtggtaatgt aaatatgaaa aatgttaata cccaggataa aaaagtcgaa gacaatgaga
     8701 ttcgctcaag tggtcttgat tcgctgtttt cgtctacttc ggagagtaaa agtaaggaaa
     8761 ataagtttaa atccgaagta aagaccgaaa aagcaagcgc taaaaaggaa attccatctg
     8821 cacaggtcca aaatcgaaga cttaaagtta taaagcctat aagttacaat tcagccgcaa
     8881 gtgaaattag cgctgcctta aaaggtggtt gtgccgtggt tttatatctt aaaggcacta
     8941 acgaagacac atcaaagcgg cttttagatt ttacatttgg tgctgcaagc atgtgtggtg
     9001 ctcacgtaag tctggatgct gaaaagacat atgttattac aattggacca gggctttcgc
     9061 aacgcgaaaa actattgtgc ataaaggaag gcgttgcctt aaaagtggaa aattaacagt
     9121 tgataaattg cctaattatg tataataggc aacacatcat aaaaaattat agaagggaca
     9181 aattatggca attacatcga gtgatattga caatcagagc ttttcggtcg agcgaaaagg
     9241 ttacagtatt gaagaagttg atatcttttt agagactgtt tctaaagagg taggcatttt
     9301 aaatgataag gttgaagagc ttgagaaagg cgcttcggag tcttcaaaag atggtgcaag
     9361 caacgaagaa gttgttcaac ttaatgctac tatcgcaaag caaagtgctg agattgaaaa
     9421 gctaaagaaa gaagttgctg aaaaggttaa cgatggaaag gccatttcgg aagccttaat
     9481 cgtcgcacaa cgtcaagctg aaaaaattgt tgctgaggct aatgacaagg ctaagaaaat
     9541 catcaaagat gctaatgaca aggctgatta cattgaaaaa gatgcagatg ctcaaaagaa
     9601 aaaagttatg caagaaattg ataaacttga aaaagaacaa caatcttgca gaaaagaata
     9661 tcaagttatg cttcgtgaca ttatcactgc gatggacaaa cgccttgatg aatcggaagg
     9721 cttatcagcg aagaaatctc cagcttcgac aaacgctaca aaggctgtta gtggatctac
     9781 atcaaagagt gctcctgtta aaagtcaagc aagcgcttcg aagactagcg aatcaaaacc
     9841 tgttcaagtt aatccagagc ctgaaaaaga tttctctggt tttggcgata cagattttga
     9901 cgatgacaaa atcgactaat tgtcaaaccc ttcaaaaaat ataattaatg ttca
//
LOCUS       DBNCDC010000032         9861 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_2933_length_9861_cov_6.970426, whole genome shotgun sequence.
ACCESSION   DBNCDC010000032 DBNCDC010000000
VERSION     DBNCDC010000032.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 9861)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9861)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9861
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2933_length_9861_cov_6.970426"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="AC04VQ_03545"
     CDS             complement(<1..649)
                     /locus_tag="AC04VQ_03545"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039482.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIO9553256.1"
                     /translation="MAVVLGGDGTMLRTSNYIGSKNIPILGINYGNLGFLTNSPDAGI
                     ENLVEDALNGKLKRESRCHLIVNVQSDAGASEFIAMNEVSIRRSKSGRIVDFAININD
                     EHVCDLRSDGVIASTATGSTAYALAAGGPLVAPTYKGVIIVPLAPHTLNSRPLVTDKD
                     DVVEFDFTKDNKDDKLAINVDGEIKIIEGKVLKVSVQTDKNETTILRYSNDTFYSR"
     gene            complement(804..3521)
                     /gene="ppdK"
                     /locus_tag="AC04VQ_03550"
     CDS             complement(804..3521)
                     /gene="ppdK"
                     /locus_tag="AC04VQ_03550"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HIO9553257.1"
                     /translation="MADKRVYAFGKDQNGVNITEGNTDMKWILGGKGANLAEMANLEL
                     PVPPGYSITCQTCMEYANAGNVWPEGALDEIWGYTENLEERMGKRIGDKDDPLLVSVR
                     SGAPFSMPGMMDTVLNLGLNDESINGLIKQTDNPRFAWDSYRRFIQMFSNVVMGIDGD
                     LFEDAINAAKKKKGVKNDTDLEAEDLKALVAKFKEIFSKNVDGKEYPSLVVNGKVEFP
                     QDPKVQLQLAIEAVFGSWNNERAVLYRQQNKIDDNLGTAVNVQTMVFGNKGDTSATGV
                     AFTRNPADGKNERYGDFLVNAQGEDVVAGIRNTSPIADLHKVDALKEASDELYAIFEK
                     LEDHFRDMCDIEFTIEQGKLWMLQTRVGKRTAHAALSIAIQMVEEGLITKEEAIKRVE
                     PEQLDQLLHPQFKKGAKYDVLAKGLNASPGAAVGKAVFSAEDAVKAKEKGVKTVLVRW
                     ETTPDDLAGMIAAEGILTSHGGKTSHAAVIARGMGAPCVCGVEALQIDAAKKQAKVAG
                     TKTTIKEGDIISIDGMTGDVVYGAVELQEAEITGDLDVMLKWADEVSRMEVHANADNP
                     HDAQLARDFGATGIGLCRTEHMFLGERKQIIQDFILSEDEKTMQKCLKDLRDAQSGDF
                     YGMFKAMDGYPVIVRLLDPPLHEFLESGRELEVELAKLETKGAAKGRIEKMKDFLDKV
                     DSFAEANPMLGMRGCRLGIMYPELTRMQVRAICTATAKLLKEGLNPKPQIMVPLVSVY
                     AELEYVRKIAEETIAEVEKDYDVKLDIPIGTMIELPRAAVTADEVAQYADFFSFGTND
                     LTQTTFGFSRDDVEAEFVPKYLKEKLLAFNPFATVDKGVAKLVSMAVELGHKGNPNIE
                     CGVCGEHGGDPDSVKVFNKIGLDYVSCSPFRVPIARLAAAQAVIEQKDE"
     gene            complement(3652..3837)
                     /locus_tag="AC04VQ_03555"
     CDS             complement(3652..3837)
                     /locus_tag="AC04VQ_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553258.1"
                     /translation="MEAAKDYPITLTGGPYVYTVNLSEKTKAAQTGDNNLPIVIALIV
                     VACACGGYVACRKLRKK"
     gene            complement(3868..4404)
                     /locus_tag="AC04VQ_03560"
     CDS             complement(3868..4404)
                     /locus_tag="AC04VQ_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553259.1"
                     /translation="MRDNNVPIEGVEASWVDPKSGTRISTYSNDLGMACLTIPYGISG
                     SIHVVRDGYVSDFSIPQTYKVGDVPSFTVVDMMKGAKNTVNSSIANTKFHVHEYVSNN
                     SIRPSFEYLAEDIYLNEVPTRMLPDGANMMVLTDAVCDKYGLNHGTLYYSYEGPLPLG
                     ASLYEIVFEPDANYKCDS"
     gene            complement(4492..4890)
                     /locus_tag="AC04VQ_03565"
     CDS             complement(4492..4890)
                     /locus_tag="AC04VQ_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553260.1"
                     /translation="MSKTKINLKLIIALFGVALVMCLVPLGVQALATDEATDAAATNN
                     EGEPAAPANDQEYTIVNQKGDDESTELGQLITRTGERKFTILDGETVETAVDGPVGYL
                     YVKHADGSKTCVAAAFSDVIYKEKLQKFKQ"
     gene            complement(5024..5557)
                     /locus_tag="AC04VQ_03570"
     CDS             complement(5024..5557)
                     /locus_tag="AC04VQ_03570"
                     /inference="COORDINATES: protein motif:HMM:NF014689.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071421 - manganese ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HIO9553261.1"
                     /translation="MFIFELIATAVGVSMDAFSVSICKGLGMKRVNFVQAAIIGLVFG
                     GFQAAMPLIGFLVVFGFQDTEEFQNAITQNAPLVTLVLLGFIGGKMIIDAVRGKSNDD
                     VARAQKLNDEGRGETKLNIIELLMLGLATSIDALMVGFAFAVIQADVILTITVIGLTT
                     FAFSFAGVFIGHFFSAR"
     gene            complement(5558..5800)
                     /locus_tag="AC04VQ_03575"
     CDS             complement(5558..5800)
                     /locus_tag="AC04VQ_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553262.1"
                     /translation="MVDYSKNMSRYDIEVAVHEALEDAYRLGMQKGEYIPLSIVNIVE
                     RLADHQPLDSSEYKKLEDVATEDFKKELENIDDILK"
     gene            complement(5852..6991)
                     /locus_tag="AC04VQ_03580"
     CDS             complement(5852..6991)
                     /locus_tag="AC04VQ_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553263.1"
                     /translation="MFGNSNDNISKKFFDLSKKATLAESEGDYLGAVNLYLAAYETVK
                     GSSEEMVKVGVEGVKKAWGIVISQNNRPLAEYIYEILCRHIKEPEIEKYSEELHNLML
                     NKLKNLGVPVEHLADTESANEALNKMKDFITGMNGKKEPEKKEPAPKEIPAPEANAKA
                     QSEKDSYYIRRELNKYDNLVGYESAIDYMRKHGVTSKENSERMQLVERLNKEHGLNRI
                     SFTETMIFTSPSRVDANLFMEATASEIGLPCIQMHMDEHVAGSSVLCMMAPADSNFRL
                     NPSRTGFNGKGILMLEDVDTWDFPDPSELSGEDGIAGFFNAQMSRGIAEVSNLIEVAL
                     EDPNILVMATVGDKVDEDEIRNSLGAETFTVIRIDNPTERERAAI"
     gene            complement(6991..7632)
                     /gene="rpmE"
                     /locus_tag="AC04VQ_03585"
     CDS             complement(6991..7632)
                     /gene="rpmE"
                     /locus_tag="AC04VQ_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013979485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIO9553264.1"
                     /translation="MKKDIHPEYMECTVTCSCGNTFKTRSTKPELKVDICNECHPFFT
                     GQQKLVDTGGRVQRFEDRFGNAKETLEAKAAEKKAARAAELEAAEADRKAKAEEKAAE
                     KEKRAKAYEAKAKKEAAQKAKEEAEKPAEEKAEDQAPAEEQAPAQEAPKAEGEATAEE
                     APKAEGEAPAEEAKPEEAPAEEKPAEEEKPAEEAKPEEAPAEEAKPEEEKPAE"
     gene            complement(7699..8304)
                     /locus_tag="AC04VQ_03590"
     CDS             complement(7699..8304)
                     /locus_tag="AC04VQ_03590"
                     /inference="COORDINATES: protein motif:HMM:NF016825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HIO9553265.1"
                     /translation="MDLDNFVEDLTSNSPAPGGGAASALFGVLGAALSSMVCALTEGR
                     SAYAEHEDFVVAQHKEILSLQEELKGMMQADVDAFMQISAAYKLPKETDAEKAKRSEA
                     IQAALEPAIKVPFKIMQLAAQGLDLTESLIGKTNAMASSDLGCAALGFKAAIQGAYLN
                     VKINLGSAKEPIGTYEEDSQAILDKYLLLADNIYNKILASL"
     gene            complement(8313..9515)
                     /gene="gltS"
                     /locus_tag="AC04VQ_03595"
                     /pseudo
     CDS             complement(8313..9515)
                     /gene="gltS"
                     /locus_tag="AC04VQ_03595"
                     /inference="COORDINATES: protein motif:HMM:NF015573.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
     gene            complement(9569..>9861)
                     /locus_tag="AC04VQ_03600"
     CDS             complement(9569..>9861)
                     /locus_tag="AC04VQ_03600"
                     /inference="COORDINATES: protein motif:HMM:NF014965.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553266.1"
                     /translation="EIRESNGGFKEVKAMGFSLDDRGITQVSMNLCDYNVTGMHIVFD
                     KISELAQKYGTEVIESELVGTAPAKAFLDVCEHYLKFKDFDAFKQMMEYRLL"
BASE COUNT         2986 a         2245 c         1672 g         2958 t
ORIGIN      
        1 ttcgactata aaaagtatcg tttgaataac gaagaattgt cgtctcgttt ttgtcagttt
       61 ggacgctaac ttttaaaacc ttgccttcga taattttgat ttcaccatca acgttaatcg
      121 cgagtttgtc gtctttgtta tctttagtaa aatcaaattc aactacgtca tctttatcgg
      181 ttacaagagg gcgcgaattt agcgtatgag gagctagcgg cacaataatt acacctttat
      241 aagtaggggc aacaagaggc cctccagcag ctaaggcata tgccgtagat ccagtagcgg
      301 ttgaggcaat tacgccatca cttcggagat cacagacatg ttcatcgttg atattaattg
      361 caaaatcaac aatacgacca gattttgaac gacgaatgct aacctcgttc atggcaataa
      421 actccgaagc tccagcatca ctttgaacat taacaattaa atggcaacga gactcacgtt
      481 tcaactttcc attcaaagca tcctctacaa gattttcaat tccagcatct ggagaatttg
      541 taagaaagcc aagattccca taattaatgc cgaggattgg gatattttta cttccaatat
      601 aattagaagt gcgaagcatc gttccgtccc caccaagaac aacagccaaa tcataagtga
      661 catcttcgtc tatcttttta ttagtaatgt ctcaaccatc aaaagtttcg caagcaatat
      721 tattatcagt gcaataggct aagacaattt cggaggcctt ggtagcctcc gaatttgact
      781 tgttcagaac tgttaaaagt ttcttactca tctttttgtt caataactgc ttgagcagct
      841 gctaagcgag caattggaac acggaatggt gaacaactta catagtcaag accaatctta
      901 ttaaagactt taacagaatc tggatcacca ccatgctcac cacaaacacc gcattcaata
      961 tttggattgc ctttatggcc aagttcaaca gccatgctaa ccaacttcgc aacaccttta
     1021 tcaacagttg caaatgggtt aaatgcaagc aatttttctt tcaaatattt aggaacgaat
     1081 tcggcttcaa catcatcacg actaaaacca aatgttgttt gtgttaaatc gtttgttcca
     1141 aaactaaaga agtcagcata ttgagcaact tcgtcggcag taacagcagc acgtggaagc
     1201 tcaatcattg ttccaattgg aatatcaagc ttaacgtcgt aatctttttc aacttcggca
     1261 attgtttcct cagcaatttt acgaacatat tcaagctcag cataaactga aacaagagga
     1321 accataattt gtggttttgg atttaaacct tccttcaaaa gctttgcagt agcagtacaa
     1381 attgcacgaa cctgcatgcg tgtcaactct gggtacataa ttcccaaacg gcaaccacgc
     1441 ataccaagca ttggattagc ttcggcaaaa ctgtctacct tatccaagaa atccttcatc
     1501 ttttcaatac ggccttttgc agcccctttt gtctcgagct tggcaagttc aacctcaagc
     1561 tcacgaccgc tttccaagaa ttcatgaagt ggaggatcaa gcaaacgaac aattacagga
     1621 taaccatcca ttgccttaaa cattccgtaa aagtcaccgc tttgagcatc acgaagatcc
     1681 ttcaaacatt tctgcatagt tttttcatct tcgcttaaaa tgaagtcctg aataatttgc
     1741 ttgcgctcgc ccaagaacat gtgctctgta cggcaaagac caataccagt agctccgaag
     1801 tcacgtgcca actgtgcatc atgtgggtta tcagcattag catgaacttc catgcgagaa
     1861 acttcgtcag cccacttgag cattacgtca aggtcgccag taatttcagc ttcttgaagt
     1921 tcaactgcac cataaacaac gtctccagtc ataccatcaa ttgaaatgat gtcgccttcc
     1981 ttgatagttg tttttgtgcc tgcaacctta gcctgctttt tagcagcatc gatctgcaat
     2041 gcctcaacac cgcaaacaca aggagcaccc atacctcgag caataacagc tgcatgcgaa
     2101 gttttaccac catgagatgt caaaatacct tcggcagcaa tcattccggc caagtcatct
     2161 ggagttgttt cccaacgaac aagaacagtt tttacgccct tttccttagc cttaacagca
     2221 tcttcagcgc taaaaacagc ctttccaaca gcagcgccag gagaagcatt caaaccttta
     2281 gctaaaacat cgtattttgc acctttctta aattgaggat gcaaaagttg atccaattgc
     2341 tcaggttcaa cacgcttaat tgcttcttcc ttagtaataa ggccttcttc gaccatctga
     2401 atagcaatag acaaagctgc atgagcagtt cttttaccaa cacgagtttg aagcatccat
     2461 agtttgcctt gctcgattgt aaattcaata tcgcacatat cgcggaagtg atcttccaac
     2521 ttctcaaaaa ttgcataaag ttcatcagat gcttccttta aagcatcaac tttgtgaaga
     2581 tcagcaattg ggcttgtatt acgaatacca gcaacaacat cttcgccctg tgcgttaact
     2641 aaaaagtcac cataacgctc atttttacca tcagcagggt tacgtgtgaa agcaacacct
     2701 gttgcagatg tatcgccctt attaccaaaa accatggttt gaacatttac agcagttcca
     2761 aggttatcat caattttatt ttgctgccta tataaaacag cacgttcatt attccagcta
     2821 ccgaaaacag cttcaatagc taactgaagt tgaactttag gatcttgagg gaattcaacc
     2881 ttgccattaa caacaagact tggatattcc ttgccatcaa cattcttgct aaaaatttcc
     2941 ttgaactttg caacaagagc ctttaggtct tcggcttcta agtcagtgtc attcttaacg
     3001 cccttctttt tcttagcagc gttgatagca tcttcgaaaa gatcaccatc aatacccata
     3061 acaacgttag aaaacatctg gataaaacgc ctgtaagaat cccatgcaaa acgaggatta
     3121 tcagtttgtt taataagacc attgatagat tcatcgttaa gtcccaggtt taaaacggta
     3181 tccatcatac ctggcatgct gaaaggtgca ccacttcgaa cactaactaa aagtggatca
     3241 tccttatcgc caatgcgctt gcccatgcgc tcttcgaggt tttctgtata tccccaaatt
     3301 tcatcgagag caccttctgg ccaaacattt ccagcatttg catattccat acaagtttgg
     3361 caagtaattg aatatccagg aggaacaggc aattccaaat tcgccatttc agctaaattt
     3421 gcacccttac caccaaggat ccacttcata tcagtgttac cttcggtgat atttactcca
     3481 ttttgatctt taccaaacgc atatactctt ttatcagcca ttttatctcc ttttttaatc
     3541 tctcaacagg gcatctatga tatctcaaaa ccaaaatttt tcacccatta attagttaaa
     3601 aaaatcgccg aagctcaagt ccccggcgat ttaaaattta tttaatatct attatttttt
     3661 acgaagtttt ctacaagcaa catagcctcc gcaagcacaa gcaacaacta tcaaagcaat
     3721 aacaattggc aaattattat ccccagtttg cgctgccttt gttttttcag ataaattaac
     3781 ggtgtaaaca tatggaccac cagttaaggt gattggataa tccttggcag cttccataac
     3841 tactgaacca cttgcatctg ttctggttca actatcgcat ttataattag catcaggttc
     3901 aaatacaatt tcataaaggc tagcacctaa aggaagaggt ccttcgtacg aataataaag
     3961 agttccatga ttaagaccat atttatcaca aacagcatca gttagcacca tcatattagc
     4021 gccgtcagga agcatacgtg ttggaacttc gttcaaatat atatcttcag caagatattc
     4081 gaaacttggt ctaattgaat tattgctaac atattcatga acatggaatt ttgtatttgc
     4141 gatactgcta tttactgtgt tcttggcgcc tttcatcatg tcgacgacag taaatgatgg
     4201 gacatcccca actttataag tttgcggaat agaaaagtca gatacataac catcacgaac
     4261 aacatgaatg ctacccgaaa ttccatatgg aatagtaagg caggccatac ctaaatcatt
     4321 tgaatatgta cttatacgag ttccgctttt aggatcaacc caagaagcct caacaccttc
     4381 aataggaaca ttattatcac gcatttgacc agcgtaataa tatccatcgc tttctactgg
     4441 agtataatca ccgtatgttg cagtatatgt aaaggtgatt gaattatagc ctcattgttt
     4501 aaatttttga agcttctctt tgtaaattac atctgagaaa gcagctgcaa cacaagtttt
     4561 tgatccgtca gcatgcttaa catataagta gccaactggg ccatcaactg ccgtttcaac
     4621 cgtttcgccg tctaatatag taaatttcct ttctccggtt cgtgtaataa gctgaccaag
     4681 ttcggtcgat tcatcatcgc ctttttgatt aacaatagta tactcttggt cgttagctgg
     4741 agctgctggt tcgccttcgt tattagtggc ggctgcgtct gtggcttcgt cagttgcaag
     4801 agcttgaact cctaatggaa ccaagcacat aacaagagca actccgaaaa gcgcaataat
     4861 taattttaaa ttgatttttg ttttggacat attatcccct ttcaaaattt gacctaaata
     4921 ttataccatt attttatttt tagaatcagc ctaagaattt agcaccgaag cgaagaccaa
     4981 taagtatcaa tactgctcca cctgcaatgg aagctggttt ttctcatcta gcgctgaaga
     5041 aatgtccaat aaatacgcca gcaaatgaaa aggcaaatgt agttaaacca attacggtta
     5101 ttgtcaagat aacatcagct tgaataacag cgaaggcaaa accaaccatt aatgcatcaa
     5161 tgctggtagc aagacccaac attaataact caataatgtt aagttttgtc tctccgcggc
     5221 cttcgtcatt tagtttttga gcgcgagcaa catcatcgtt gcttttacca cgaacagcat
     5281 caattatcat ttttccacca ataaaaccaa gaagcacaag tgtgacaagc ggtgcatttt
     5341 gagtaatggc gttttgaaat tcttcggtgt cttgaaaacc aaacacaact aaaaagccaa
     5401 taagcggcat agctgcttga aaaccaccaa aaacaagtcc aataattgca gcttgaacaa
     5461 agtttacacg cttcattcca agtcccttac aaatagagac tgaaaaagcg tccattgaaa
     5521 cgccaacagc agtagctatt aattcaaaga taaacattta ctttaagatg tcgtcgatgt
     5581 tttcgagttc ctttttaaaa tcctcggttg caacatcttc gagttttttg tattcagaag
     5641 agtcgagcgg ttgatggtcg gccaagcgct caacaatatt tacaattgaa agaggaatgt
     5701 attcaccctt ttgcattccc aaacgataag cgtcttccaa ggcctcgtga acagcaactt
     5761 caatgtcata acgagacata tttttggaat aatctaccaa tttttgagtg ctaaaagcac
     5821 gaagagatgg atggtccgct gcaagttttg ctcatattgc agcgcgctca cgctctgttg
     5881 gattatcgat gcggataacc gtgaatgttt cagcaccaag actgttgcgg atttcgtctt
     5941 cgtcaacttt atcccctaca gttgccataa ccaaaatgtt tggatcttcc agcgcaactt
     6001 cgataaggtt cgaaacctcc gctattcccc tgctcatttg tgcgttgaaa aagccggcaa
     6061 ttccgtcttc gccactaagt tccgaaggat caggaaaatc ccaagtgtca acgtcttcga
     6121 gcatcaaaat acccttgcca ttaaaaccag tgcgacttgg gtttaagcga aaattgctat
     6181 cggccggagc catcatgcac aaaacacttg aaccagcaac gtgttcatcc atatgcattt
     6241 gaatgcaagg aagaccaatc tcagaagcag tagcctccat gaacaaatta gcatcaacgc
     6301 gcgaaggcga tgtaaatatc atagtttctg taaaagaaat tcggtttagg ccgtgttctt
     6361 tatttaagcg ctcaacaagt tgcatgcgtt ctgaattttc tttagaagta acaccatgct
     6421 tgcgcatata atcaattgca ctttcataac caaccaaatt gtcatatttg tttaattcgc
     6481 gacgaatata gtaagaatct ttttcgcttt gagcttttgc attagcttca ggagcaggaa
     6541 tttccttagg agcaggctcc tttttctctg gctccttctt gccgttcatg ccagtaataa
     6601 aatctttcat tttattcaaa gcttcatttg cagattctgt gtctgctaaa tgctcaaccg
     6661 gaactcccaa attcttaagt ttattcaaca ttaagttgtg aagttcttca gaatattttt
     6721 caatttcagg ctctttaatg tgcctgcaca aaatttcgta gatatattca gcaagcggcc
     6781 tgttgttttg agaaataaca atgccccatg ccttctttac tccctcaacg ccaactttaa
     6841 ccatttcttc gctgctacct ttaacagttt cataagcagc aaggtatagg tttacagcac
     6901 ctaagtaatc gccttcgctt tctgcaagcg tggctttttt acttaggtca aaaaactttt
     6961 tacttatgtt gtcgttggag ttaccaaaca ttattcagct ggtttttctt cttcaggttt
     7021 agcttcttct gcaggagctt cttctggttt agcttcctca gctggttttt cttcttcagc
     7081 aggtttttct tctgcaggag cttcctcagg tttagcttct tcagctggag cttcaccttc
     7141 agcctttgga gcttcttctg ctgtagcttc gccttctgct ttaggagctt cttgtgctgg
     7201 agcttgttcc tcagcaggag cttgatcctc agctttttct tcggctggtt tttcagcttc
     7261 ttccttagcc ttttgagctg cttctttctt agccttagct tcataagcct tagcacgctt
     7321 ttctttctca gcagcctttt cttcggcctt agccttgcga tcagcctctg cagcttctaa
     7381 ctctgcagcg cgtgcagcct ttttctcggc agcttttgct tccaaggttt ccttagcatt
     7441 tccgaagcgg tcttcgaagc gctgaacacg tccaccagta tcaacaagtt tttgctgacc
     7501 agtaaagaac ggatgacatt cattgcaaat gtcaacttta agttctggct ttgttgaacg
     7561 agtcttgaat gtgtttccac atgaacaagt aacggtacat tccatgtact ctgggtgaat
     7621 atcttttttc atttttcaat cctttccgtt ttgatttccg accaaaactc gcggactgcg
     7681 tattttacca cgaaaatact ataatgaagc taaaatttta ttataaatat tatctgcaag
     7741 tagcaggtat ttatccagta ttgcttggct gtcttcttcg taagttccga ttggttcttt
     7801 cgcggaaccg aggttgattt ttacgtttaa gtaagcgcct tgaattgccg ctttgaatcc
     7861 aagtgcagcg catccaagat cgctggatgc cattgcgtta gttttaccaa ttaagctctc
     7921 ggtcaaatct aaaccttgag cagctaattg cattatctta aaagggactt tgattgctgg
     7981 ttcaagagca gcttggatgg cttcgcttcg ctttgccttt tccgcgtcag tttccttagg
     8041 caatttataa gctgcgctga tttgcatgaa tgcatcgacg tctgcttgca tcattccttt
     8101 tagttcttcc tgtaatgaaa gtatttcttt gtgttgcgct acaacaaaat cttcatgttc
     8161 tgcatacgca gaacgacctt cggtcagggc acaaaccata gaagataatg cagctcctaa
     8221 aacgccgaag agtgccgaag ctgctccacc acctggagct ggactattac ttgttaaatc
     8281 ctctacaaaa ttatctagat ccataacatc tcctaaccta caactggaca aaaaacattt
     8341 aataaagtaa tcgtggttgg ctggttaata atgtcaacta aaaatgatcc acaaattgga
     8401 acaattaaaa atgctgtttt actatagccg aagcgctcac aaactgcgtt catatttgcc
     8461 attgcagtag gagttgctcc aagcatatga ccacaagctc cagcacacat aattgccgca
     8521 tcataatctt taccaagaac cttaaacata atgaagtatg atgtagtaaa cataagcaat
     8581 gcctgagcaa ttacaataac tgccaaacca ccaacaagac ccaaaagctg ccataattta
     8641 atagacatca ttgcaattga caagaacaaa tcaagagtca cagctccaac tacatcactt
     8701 accttctggc tgtaattata ccaatgaaat ttttcattta aatttcgaac aagaacagat
     8761 acaatcattg cgccaatata atccggcaaa gcaatggcta cgccagttgt atactcaaga
     8821 actcaggata taccactagc aagcataatc ccaaaagtca tacaaagcaa aataacaaca
     8881 aaattttgca ttatttctgg cattgatagt tcttccttta tctgcttggc tgtttcaaca
     8941 acactaacct tggatgcttt tggcttaagt ttatttttaa ctatgagtct gcgagaaaga
     9001 ggccctccaa ccaacacaga aacaattaag ccaaaagttg cagcagccaa cccacaatcc
     9061 tgagctgctt cgtaacccct atcaataaga gttgatccat aagcagttgc cgcaccatga
     9121 cctccgcaca ttgacatagc tccagccgta atgctataac cattttctat acccattaaa
     9181 ttacccaatc caagagcaag tggagtttgg ataacagaaa taatagagac acaaactcaa
     9241 taaattagca taactcttcc accaacttta aataacttaa agtcagctcc caaacctata
     9301 gtgctaaaaa aagcaaccat aaaaattgtt tgaaacgtag tgtcaaaatt tatagcgaaa
     9361 ctatttgtct ggtgtccaac aaaaacaaga atcataaata taaagccacc aacaacaggt
     9421 gaagggatgc aatatttttg taaaaattta attttcttac aaattgcaat accaattaac
     9481 aatacaacgc ctgccaaagc aagggttgaa atcatgtcta aattaacagt aaaaacagaa
     9541 tcaacaaaat ctcaattcat ttaaacccct acaataatct gtattccatc atttgtttaa
     9601 atgcatcaaa atctttaaat ttaagatagt gctcacaaac atccaaaaat gctttagctg
     9661 gagctgttcc aacaagttca ctttcaataa cttcggtgcc atatttttga gcaagttcag
     9721 aaatcttatc aaaaacaatg tgcatgccag taacattgta gtcgcacaaa ttcatcgaaa
     9781 cctgagtgat gcctctgtca tccaaactaa agcccatagc tttaacttcc ttaaagcctc
     9841 cattgctctc gcgaatttca c
//
LOCUS       DBNCDC010000033         9848 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_2939_length_9848_cov_7.001532, whole genome shotgun sequence.
ACCESSION   DBNCDC010000033 DBNCDC010000000
VERSION     DBNCDC010000033.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 9848)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9848)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9848
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2939_length_9848_cov_7.001532"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(38..199)
                     /locus_tag="AC04VQ_03605"
     CDS             complement(38..199)
                     /locus_tag="AC04VQ_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553267.1"
                     /translation="MFPRVQSGHKHDYKIKGLRFVGGHGTQMDGYSQAYLSGREQARY
                     MLDDIERGI"
     gene            complement(263..1591)
                     /locus_tag="AC04VQ_03610"
     CDS             complement(263..1591)
                     /locus_tag="AC04VQ_03610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIO9553268.1"
                     /translation="MPKKTYDNYDIVVVGAGNGGLSAAAYMAQQGKRVLVLEQHNAPG
                     GCATSFVRGRFEFEATLHEMCQMGEGENAGAVRQLLDSYGLDVDWVAVEEAYASINTD
                     KETGFNVVMPVGVQEYIDEMERQVPGSRESMETVMEFGRMICDGVEWLDKHNNEPGPL
                     AKVEMMLKYKDLMKIVPVPCDTMLRKIGVPDKAREIYESYWDYVGVDSTKMSFAVYAF
                     MVYTYLTKKPWIAKKASHEISIAFDKAIRDMGGDIWYNTEVTKIDVQGGEVKGVETWD
                     GAYIKCDRVISNLMPTVVYDRLVDPDEVTEYDKKLMNARKLAQSAFTIYCALDIPYEK
                     LGFKPYDTFVRTTGDTLQQYNNAATLEGHKDYCVTIVNAANPECTPKGTCTVQFAKFY
                     TEDAFADIKVEDYFKVKDQIARETIEDFEKVVGIDIMNHIEEIEIATPET"
     gene            complement(1636..1854)
                     /locus_tag="AC04VQ_03615"
     CDS             complement(1636..1854)
                     /locus_tag="AC04VQ_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIO9553269.1"
                     /translation="MSTLDTIKDILKEKQDIDPASVNENSTFEDLKIDSLDLVEMVCE
                     LEDRMKIDFGDPEGLKTIGDVVEYVDAL"
     gene            complement(1857..2612)
                     /locus_tag="AC04VQ_03620"
                     /pseudo
     CDS             complement(1857..2612)
                     /locus_tag="AC04VQ_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012379.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
     gene            complement(2625..>3143)
                     /locus_tag="AC04VQ_03625"
     CDS             complement(2625..>3143)
                     /locus_tag="AC04VQ_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012379.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIO9553270.1"
                     /translation="GFIDVETPILANPTPEGARDYIVPSRVNPGSFYALPQSPQQFKQ
                     LLMVAGVERYYQIARCFRDEDLRADRQPEFTQVDVEMSFVHTSEVIDAMEEVILEALN
                     ECGANVKGPLIRMDYSEAMERFGCDRPDNRFGMELVDISDLVKDSDFKVFSNTVNDGG
                     VVKAINAKGAGD"
     assembly_gap    3144..3243
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3244..3473)
                     /locus_tag="AC04VQ_03630"
     CDS             complement(<3244..3473)
                     /locus_tag="AC04VQ_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HIO9553271.1"
                     /translation="MANKDFANMYCLHTATCGQLRAKDINSEVTLCGWIWRNRDLGGF
                     CFITLRDRTGITQVVIDPDVLGKEAFEEISHYG"
     gene            complement(3473..4717)
                     /gene="hisS"
                     /locus_tag="AC04VQ_03635"
     CDS             complement(3473..4717)
                     /gene="hisS"
                     /locus_tag="AC04VQ_03635"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIO9553272.1"
                     /translation="MFQQKARDVFGRYGYVEIATPFFEQTDLFVRGIGEATDVVSKEM
                     FRVISGGNLEKVFAGEQIKAKSNLSLRPEGTAGVVRAVIQNGMLPQGSAPLKLMYAGP
                     MFRAERPQDGRQRQFMQIGVECLGASEASIDAEGIIMLMRFYEALGFETKKLNLVLNS
                     MGCPNCRANYRKDLLEFLKKNEKNLCETCVERIEKNPLRTLDCKDEKCQKVLDGAPKI
                     SDYLCDDCKNHYEEVKSLLKNSGIKFVEDWKLVRGLDYYTRTVFEIQSGDVGAQDALC
                     GGGRYDGLVKELGGNDTPGFGFALGYERTLLAMKNNGFEFEPAKTCDYFVAIASDGAK
                     EKAFEVAQSLRDYGCNVEIDHQSRSLKSQFKLADKFMAKFTIIIGEDELKKGELCVRN
                     METHDQENIKQDKLFAFVEGKL"
     gene            complement(4872..5771)
                     /locus_tag="AC04VQ_03640"
     CDS             complement(4872..5771)
                     /locus_tag="AC04VQ_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HIO9553273.1"
                     /translation="MVKESNKKDSGQQSKIIVRKGDSGAATEDVQRKLNKLGYLKDRQ
                     IDSFYGDKTAEAVQKFAEDNGLAPVEDVTEKVWAKLVDASFKLGDRNLFLRIPYFHGN
                     DVLELQKALGSLGFQVRNYDGIFGTSTERALRRFQINMGLPTDGIAGSQTFMALRNLH
                     FTWSDKSPLDKDKEVEVNAVWRASEVLEQNSICLFGTDDFTRDVAIRMSNLARATNPF
                     SKILSSNAYSIAPDKSTMLLHIVLPEEKDIKGQSIPRVLYAENEEQEENVSEAFIKRL
                     TSAITAAKKLSPARLSIELPGKA"
     gene            complement(5802..6848)
                     /locus_tag="AC04VQ_03645"
                     /pseudo
     CDS             complement(5802..6848)
                     /locus_tag="AC04VQ_03645"
                     /inference="COORDINATES: protein motif:HMM:NF013695.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
     gene            complement(6970..8049)
                     /locus_tag="AC04VQ_03650"
     CDS             complement(6970..8049)
                     /locus_tag="AC04VQ_03650"
                     /inference="COORDINATES: protein motif:HMM:NF015948.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIO9553274.1"
                     /translation="MEPSKVYFTDFHCSQRQSRLSKLRNLIKRAGMDQIDFKDKFVAV
                     KIHFGEHGNLGFIRHQYAKVVCDFIKERGGKPFLTDASTLYIGMRNNAVDHLENAFLN
                     GFNPLSTGVPTIIADGLRGTDERLIKVQGAKHCKVARVASAIAEADVLITMTHTKGHQ
                     VAGYGGTLKNIGMGCGSRQGKMEMHSSDTPMILKKRCIGCGSCVAHCAHKGVSIVKGK
                     AVINEKNCVGCGYCFCYCPKGAINCKWDEATTVMVEKVAEYTKAAIDDKPAFHINFVT
                     NVTKHCDCKPSNDVSIIPDIGMFASFDPIALDQACVDAINSSVIFEDSELGEAAKEAG
                     ETAENGIIAAGEGHNVFQMVHPDTH"
     gene            complement(8100..8945)
                     /locus_tag="AC04VQ_03655"
     CDS             complement(8100..8945)
                     /locus_tag="AC04VQ_03655"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIO9553275.1"
                     /translation="MGKLARPLTFLATYLAANCGDDSQIEDVLPIACAIENFQTAALI
                     HDDIADGGQLRRGKPCMYLEEGEGIAINAGDFGLASTVGGVVEALRQSKFSKTETLYI
                     VRQLIFMEYMTIEGQAMDLGWARDGRFDISEDDYICMATKKSAYYSVAIPCVLGAYCA
                     DAPEELCLALKSFGEKLGVAFQIQDDLLNLIANENSENVKDFRNDITEAKRTLVVVKA
                     LELSKESDKLTQILKKNTSDEAELEKAVQIMIECGAIDYAKDKAKNLSEKAKNILEDA
                     LPSSQ"
     gene            complement(8955..9125)
                     /locus_tag="AC04VQ_03660"
     CDS             complement(8955..9125)
                     /locus_tag="AC04VQ_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553276.1"
                     /translation="MSLNFDKKIDSFEQYLKETAPVLQKYLLQNLDNVISTFEADKHK
                     DVDKYLYDPLRA"
     gene            complement(9125..9283)
                     /locus_tag="AC04VQ_03665"
     CDS             complement(9125..9283)
                     /locus_tag="AC04VQ_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553277.1"
                     /translation="MNNLIYAGLGSDICLTMVDGDVVYKDGTWPHLDIEKIKAEVKDR
                     RHRIIGEL"
     gene            complement(9302..9649)
                     /locus_tag="AC04VQ_03670"
                     /pseudo
     CDS             complement(9302..9649)
                     /locus_tag="AC04VQ_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            complement(9668..>9848)
                     /locus_tag="AC04VQ_03675"
     CDS             complement(9668..>9848)
                     /locus_tag="AC04VQ_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIO9553278.1"
                     /translation="ICREIGQMAKDKNLIIHSHVSETQAEHEECKERHNGLTPIQYFE
                     SIGVLDAQNLIAHGD"
BASE COUNT         3033 a         2316 c         1493 g         2906 t
ORIGIN      
        1 cctttaaggg aacttttatc ataattatac ttagccatta tatccccctt tcaatgtcat
       61 cgagcatgta tcttgcctgt tcacgaccgc tcaaataagc ttggctataa ccatccattt
      121 gagttccatg acctcccaca aaacgaagcc ctttaatttt gtaatcatgt ttatgcccgc
      181 tttgaacacg agggaacatt ccgtctcaag ttccacaagc ataaccataa acatctccct
      241 gaggagtacc taaatatcta gctcaagttt caggcgttgc aatttcaatc tcttcaatat
      301 gattcataat atcaatgcca acaacctttt caaaatcttc aattgtctct cgcgcaattt
      361 gatccttaac cttaaagtag tcttcaacct taatatcggc gaaagcgtct tcggtgtaga
      421 acttcgcaaa ttgaactgtg caagttccct ttggagtgca ttctggattt gcagcattaa
      481 caattgttac gcaataatct ttatgacctt caagtgttgc agcgttgtta tattgctgca
      541 aagtgtcacc tgttgttcgt acaaaagtat cgtaaggttt aaaaccaagc ttttcgtaag
      601 gaatatccaa tgcgcaataa attgtaaaag cactttgcgc aagtttacga gcattcatta
      661 attttttgtc atattcggta acctcatcag gatcaacaag tctgtcataa acaacagtag
      721 gcataagatt agaaattact ctatcgcatt taatgtaagc tccgtcccag gtttcaacac
      781 ccttaacttc tccaccttga acatcaatct ttgtaacttc agtgttgtac caaatatctc
      841 cacccatatc acgaattgct ttatcaaacg caatactaat ttcatgactt gcctttttag
      901 ctatccaagg ttttttggtt aaataagtgt agaccataaa agcataaaca gcgaagctca
      961 tcttcgtgct gtcaacacca acataatccc aataagattc gtagatttca cgagccttat
     1021 ctggaacacc aatcttacga agcattgtgt cgcaaggaac tggaacgatc ttcataaggt
     1081 ctttatattt gagcatcatc tcaacttttg caagtgggcc tggttcgtta ttgtgcttat
     1141 ctagccattc aacaccatcg caaatcatgc ggccaaattc cataacggtt tccatgctct
     1201 cacggcttcc tggaacttga cgctccattt catcaatgta ttcttgcaca ccaactggca
     1261 ttacaacatt aaatccagtt tctttgtcag tgttaatcga agcataggct tcctcaaccg
     1321 caacccaatc aacatccaaa ccataagaat caagcaattg acgaacagcg ccagcatttt
     1381 caccttcacc catctgacac atttcgtgaa gcgttgcttc aaattcaaag cgcccgcgaa
     1441 caaagcttgt ggcacaaccg ccaggggcat tgtgctgttc taagacaaga acgcgtttgc
     1501 cttgttgcgc catgtaagca gcagcagaaa gaccaccatt accagcgcca acaacaacaa
     1561 tgtcgtagtt gtcgtacgtc tttttaggca tttttcctcc ttaaatttat agcccaatta
     1621 ttttatcata agcatttaaa gggcatctac atattcaaca acgtccccaa tggtttttag
     1681 gccttcggga tcaccaaaat caattttcat tctatcttca agttcgcaaa ccatctcaac
     1741 caaatcaagc gaatcaattt taagatcttc aaaagtggaa ttttcgttaa cgcttgctgg
     1801 atcaatatct tgcttttctt ttagaatatc tttaattgtg tcaagagtac tcatgtctac
     1861 tcctttactg ttgtcttaat tgaaagttca tctaaacttt tatcatcaac tgggtttggc
     1921 gcacctgtca ttaagtccga agcactggtc gtcttcggaa atgcaatcac atcacgaatt
     1981 gaatcacgtc ctgccaaaag cattaccatt ctatctaagc caagtgcaat accaccatgc
     2041 ggaggcgcac ctaaacttaa agcatgaagc aaatgtccaa acttcaagtc aatttcttcg
     2101 tcacttagac cgcaaactcg aagcacgcgt cgctgctctt ctggattatg aatacgaatc
     2161 gaaccaccgc cagcttcgta gccatccata ataatgtcat aactataact tccacattca
     2221 agcggagcgc tttcaatttt gtccatatcc tcatccaaaa catgagtgaa aggatgatgt
     2281 tctgcactgt actttccgct ttcttcgtct tgacaaaaca ttggaaaatt tacaacccac
     2341 aaaagcgaat gcttcgactt gtcaattagc ccaagttcat ttcccatatg aatacgaagc
     2401 gcacttaaag ccgcacttac aaccttatat ttatcagcaa caaacaaaac taggtctcca
     2461 tcattgacat ccaaagcctt tttcaaatca gccatttgtt tgtcatcgaa aaacttagca
     2521 atagaacttg tgattgaacc atcacttcta aatagcatat aggcaagccc cttagcacca
     2581 ttatcagttg caatcttttg aagcttttcg gtgtcgcctc ggcttcaatc acccgcacct
     2641 tttgcattaa ttgcttttac aactccaccg tcattaacag tgttgctaaa aactttgaaa
     2701 tcactatctt taacaagatc tgaaatatcg acaagttcca taccaaatct attatccggc
     2761 ctatcacacc caaagcgttc catagcttcg gagtaatcca ttcgaattaa cggaccctta
     2821 acatttgcac cacattcatt caatgcctca aggatcactt cttccatcgc atcaattact
     2881 tctgatgtgt gaacgaagct catctcaaca tcaacttgag taaattcagg ctggcgatca
     2941 gcgcgcaaat cttcgtcacg aaaacaacga gcaatttgat aataacgctc aaccccagca
     3001 accatcaaaa gctgcttaaa ttgttgagga ctttgaggaa gcgcataaaa acttcctgga
     3061 ttaacacgac ttggaacaat gtagtcacgc gcaccttcag gtgttggatt agccaaaatt
     3121 ggagtttcaa catcaataaa accnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnccatagt gagaaatttc ttcaaaggct tccttaccca aaacatctgg gtcaattaca
     3301 acttgagtaa ttccagtgcg gtctcgaagc gtaatgaaac aaaaaccacc aaggtctctg
     3361 ttacgccaaa tccaaccaca aagtgtaact tcgctgttaa tatctttagc gcgaagctgc
     3421 ccacatgttg cagtatgcaa acaatacata ttcgcaaaat ctttattagc cattagagtt
     3481 tcccttctac aaatgcgaat aatttatctt gtttaatatt ttcttggtca tgagtttcca
     3541 tattacgaac gcaaagttca cccttcttta gttcatcttc gccaataata attgtaaact
     3601 tcgccataaa tttatccgca agcttaaatt gactttttaa acttctgctt tgatgatcaa
     3661 tttctacatt acatccataa tcacgaagcg attgcgcaac ctcgaaagct ttttcctttg
     3721 ccccgtcact tgcaatagct acgaagtaat cgcaagtctt cgcgggttca aattcaaagc
     3781 cattattttt catggcaaga agtgtgcgct cataaccaag agcaaaacca aaacctggag
     3841 tgtcatttcc tccaagttct ttaaccaatc catcatagcg gccaccgcca caaagtgcat
     3901 cttgagctcc tacatcgccg ctttgaattt caaaaacagt tcgcgtgtaa taatcaagac
     3961 cacgaaccaa tttccaatct tcaacaaact taattccaga atttttaagc aagcttttaa
     4021 cttcttcgta gtgatttttg caatcatcac ataaataatc tgaaattttt ggagccccat
     4081 ccaaaacttt ttggcatttt tcatctttgc aatctaaagt tcgaagcgga ttcttctcta
     4141 ttctctcaac acaagtttca cacaaattct tttcgttctt tttcaaaaat tcaagtagat
     4201 cttttctgta attagcgcga caattcgggc atcccatcga attaagaaca agatttaact
     4261 ttttagtttc aaaaccaagc gcctcataaa aacgcatgag cataattatg ccctcagcat
     4321 ctatcgaagc ttcagacgca cccaaacact caacaccaat ttgcataaat tgacgctgac
     4381 gaccatcttg tggacgttct gccctaaaca taggaccagc atacatgagc tttagaggag
     4441 ctgaaccctg tggaagcatt ccgttttgaa taacagctcg aacaacgcct gcggtgcctt
     4501 cgggacgaag acttagattg ctcttagctt taatttgctc tcctgcaaaa accttctcca
     4561 agttcccgcc agaaattaca cgaaacattt ccttacttac aacatctgta gcctcgccaa
     4621 tgcctcgaac aaacaaatca gtttgctcaa agaatggcgt agcaatctca acataaccat
     4681 aacgaccaaa aacatcgcga gctttttgtt gaaatatggt tcaaaacttt gcttcgtcag
     4741 gcaaaatatc tactgttcca agtggtgaat taatcatttt tcctccgtga aacattttct
     4801 aatgcagaac acaatgcatc taaagttgtt attgcaaaat gctgggcgct tcggtcttcg
     4861 cctgcatcaa ctcaagcttt tccaggcaac tcaatagaca aacgcgccgg acttaatttc
     4921 ttcgcggcag taatagcact agttaatctt ttaataaaag cttccgaaac gttttcctct
     4981 tgctcttcgt tttcggcata taacactcga ggaatgcttt gccctttaat gtctttttct
     5041 tcaggcaaaa caatatgaag aagcatcgtg cttttatccg gagcaatact ataagcattt
     5101 gaacttaaaa ttttggaaaa aggatttgtt gcgcgtgcca aattcgacat acgaattgca
     5161 acatcgcgag taaaatcatc cgtcccaaaa agacaaatcg aattttgttc aagaacctca
     5221 cttgcacgcc aaacagcatt aacttcgact tctttatcct tatcaagagg agatttatca
     5281 gaccaggtaa agtgcaaatt tctaagcgcc ataaaagttt gtgaaccagc aatgccatcg
     5341 gttggtagcc ccatattaat ttgaaaacgg cgaagcgcac gctctgtgga agttccaaaa
     5401 atcccatcat aatttcgaac ttgaaaacct agcgagccaa gtgccttttg aagttcaaga
     5461 acatcatttc catgaaaata aggaatgcga agaaataagt ttctgtcacc aagcttaaaa
     5521 cttgcatcaa ctaacttagc ccaaactttt tcagtaacat cctcaacagg agccaaacca
     5581 ttatcttcag caaatttttg aaccgcctca gcagttttat caccataaaa tgaatcgatt
     5641 tgtctatctt ttaaatagcc aagtttgttt aacttgcgct gaacatcttc ggttgcggcc
     5701 ccactatcgc cctttcgaac aataattttc gactgctgac cactatcttt tttgtttgac
     5761 tcttttacca tatcaaacaa gatattatac tacattttga tttattcttc gagctcagct
     5821 tcggcttctt cgataagctc ttctaagacc tgtttttgat gatgaacaga ctgaaaactc
     5881 tcaacttcta taaaaattct tcgtgcttct ggatatttgg cagtaataca cttttcaatt
     5941 ctatcgatag aacgaagcac ttgtacttcg cttgcatcct ttttgaaatt aacgtccaaa
     6001 gtcaagatca tatcttgcgg acccatatac atcgaaagta cctgaccgca cctgttaaca
     6061 cattcatcct tttctacaat tttaatgatg tcctcaatct cataaggaga caggccttcg
     6121 ccaattaaca aacctttagt ttctcgcaaa agtaaaattg caacaacaac taaaagcaaa
     6181 ccaataatta tcgaagcagc ggcatcaaat cgagcgtcgc cagtaacatt agaaagaaaa
     6241 acgccaacaa ttgccacaag caaacctatt tcagctgcgc tgtcttcaaa taaaattaca
     6301 aaatttgaag gatccttaca agtgcgaaca tattttgcaa tgctcatcga gccacgctct
     6361 ttattaacag tgtgaattgc aataccaaga gaagttcctt cgataatcat cgcgattaaa
     6421 caaacaatat aattaagcgt tggatcacca agaaccattt tatcagcgtt aattcaagca
     6481 gtcacacctt tgaaaattga aacaccacca cctagtgcaa aaataaaaag tgcaacaata
     6541 aatgtaaaga agtaaagttc cttgccatat ccaaatggat ggtcaatgtc aggttttcgc
     6601 tttgaagcgc gaattccata caaaactaaa attccgtcgc ctgagtcaac aagcgagtga
     6661 attccttcag caaccatcgc cgaagatcca gaaattccag cagcaacaaa tttcacaaga
     6721 ccaattaata tatttccaac aattgctgca attatagcaa ccggcgaaga ctcattgtcg
     6781 cctcgccttg cattacgtgc cttggttgag cgctgttgca ttactgcttt ttcgaaatct
     6841 ttttccattt tacactatct acctgcataa ggtgtaattc ttgccaggca ataattacac
     6901 ctattttttc tttagttcgt atttacgagt accaagacca agtttttcac catgaataag
     6961 tccagcctct caatgagtat caggatgaac catttgaaaa acattatggc cctcacccgc
     7021 agcaataatt ccattttcgg ccgtctcgcc ggcttcttta gcagcttcac ctaactcact
     7081 atcttcaaaa attacagatg aattaattgc atcaacacaa gcttggtcca aagcaattgg
     7141 atcaaaactt gcaaacattc cgatgtcagg aataatcgaa acatcattag aaggcttgca
     7201 gtcgcagtgt ttcgtaacat tagtaacgaa gttaatatgg aaagccggct tatcatcaat
     7261 ggctgccttt gtgtattcgg caactttttc aaccataaca gttgtagcct catcccactt
     7321 acaattaatt gcacccttag gacaatagca aaaacaataa ccgcaaccaa cgcaattttt
     7381 ttcattaatc acagctttgc ctttaacaat gctaacaccc ttatgtgcac aatgagcaac
     7441 gcaacttccg caaccaatgc atcgcttctt taaaatcatc ggagtatcag aactgtgcat
     7501 ttccattttt ccttgacgtg aaccacagcc cataccaata tttttaagcg tgccgccgta
     7561 gcccgcaact tgatgaccct tagtgtgagt cattgtgatt aaaacatcag cctctgcaat
     7621 cgccgaagcc acacgcgcaa ccttgcagtg ctttgcccct tgaactttaa tcaaacgctc
     7681 atcagttcct cgaagaccat cggcaatgat tgttggaacg ccggttgata aaggattaaa
     7741 accatttaaa aatgcgtttt ccaaatgatc cactgcgttg tttctcatac caatataaag
     7801 tgtgcttgca tcggtcaaaa atggcttgcc accacgttct ttaataaagt cacaaacaac
     7861 cttcgcatat tgatgacgaa taaatccaag gtttccgtgc tctccaaaat gaatttttac
     7921 agcaacaaat ttatctttaa aatcaatctg atccatgcct gctctcttaa tcaaattacg
     7981 aagtttcgaa agcctgcttt gacgttgaga acaatggaaa tcagtaaaat aaacttttga
     8041 tggttccatt actttcccct ttcaactgct cagttgcaca tatctaataa tatatctttt
     8101 cattgcgaag aaggcaacgc atcttctaaa atattttttg ccttttcgct taaattcttc
     8161 gccttatctt ttgcataatc gatcgcaccg cactcaatca taatttgcac agccttttca
     8221 agttcagctt catcacttgt attcttcttc aaaatttgag ttaacttatc gctttctttt
     8281 gaaagttcaa gagccttaac cacaaccaag gttcgcttgg cttcggtgat atcatttcga
     8341 aaatctttta cgttctcaga attttcatta gcaattaagt tcaacaaatc atcttgaatt
     8401 tgaaaagcaa caccaagttt ttcaccaaaa cttttaagcg ccaagcaaag ctcttccgga
     8461 gcatcggcac aatacgcacc caaaacgcaa ggaatagcaa cagaatagta agcagatttt
     8521 ttagtggcca tacaaatata atcatcttca gaaatatcaa atcgaccatc acgagcccat
     8581 cctaaatcca tagcctgacc ttcaatagtc atatattcca taaaaattaa ttgccgaaca
     8641 atgtataaag tttcagtctt tgaaaacttc gattgacgaa gtgcttcaac aacacctcca
     8701 acagttgaag ccaaaccaaa atctccggca ttaattgcaa tgccttcgcc ttcctctaaa
     8761 tacatgcaag gttttccacg acgaagctga cctccatcag caatatcatc atgaataagt
     8821 gcagcagttt gaaaattttc aatagcacaa gcaattggca agacatcttc aatttgcgaa
     8881 tcatcaccgc aattcgccgc caaatatgtt gccaaaaaag ttaagggacg cgcaagtttt
     8941 ccaatatttt ttgttcaagc gcgaagtgga tcatataaat acttatcaac atccttgtgc
     9001 ttgtccgcct caaaagtaga aattacatta tccaagtttt gaagcaaata tttttgcaaa
     9061 acaggcgcag tttcttttag gtattgctca aaggaatcaa tctttttatc aaaattcaaa
     9121 gacattaaag ctcacctata attctatgtc ttcgatcttt aacttcagct ttaatttttt
     9181 cgatatccaa atgtggccaa gtgccatctt tgtaaacaac atcgccatct accatcgtaa
     9241 gacaaatatc agacccaagg ccagcgtaaa ttaaattgtt caaaatatta tctccgggac
     9301 atcaaacaag ctcgcttaaa tccataacag tcaaatcagc cttatagccc ttcttaataa
     9361 gaccacaatt ttttcttcct tgtgcaacag caccatttac agtagcaatt ttatatacaa
     9421 tgtccgctgg catcaacgta gaatcatgag catgccctct attaagtaat gccataatat
     9481 acatatcttg caagaaatta tgattgttgt ttgaacccat tccatcggtg cctagaccaa
     9541 tatttacacc atgatttaaa aattcaggaa gttcagcaat gccacttccc aactttaaat
     9601 ttgaagcagg acatgttgca actgttacat tgtgagcttt cataatcttt aaatcttctt
     9661 cttcgagtca atcaccatga gcaattaaat tttgagcatc aagcacccca atactttcaa
     9721 aatattgaat tggagttagt ccattatggc gctctttgca ttcttcatgt tcagcttgag
     9781 tctcagaaac atgactgtga ataattaaat tcttatcctt tgccatctgc ccaatttcac
     9841 ggcaaatt
//
LOCUS       DBNCDC010000034         9774 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_2993_length_9774_cov_8.163494, whole genome shotgun sequence.
ACCESSION   DBNCDC010000034 DBNCDC010000000
VERSION     DBNCDC010000034.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9774)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2993_length_9774_cov_8.163494"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="AC04VQ_03680"
     CDS             <1..141
                     /locus_tag="AC04VQ_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIO9553279.1"
                     /translation="AQTGDENGMLIAILIAVVALAIVGLASYFVINKVKNNKAKSKHP
                     KH"
     gene            289..705
                     /locus_tag="AC04VQ_03685"
     CDS             289..705
                     /locus_tag="AC04VQ_03685"
                     /inference="COORDINATES: protein motif:HMM:NF017088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HIO9553280.1"
                     /translation="MIYLSRLIGKPVVDCEGKRLGSVSDVAISTGEVFPRITSLAFLG
                     PGKTPFMISWRKFVEEFDEDDGIKLKLPQPQIRFSYLQPDEVLLARDLLNRQIVDTQG
                     LKVVRVNDLKLSQYGNQLRLLGAEVGIRGLLRGLAG"
     gene            781..1551
                     /locus_tag="AC04VQ_03690"
     CDS             781..1551
                     /locus_tag="AC04VQ_03690"
                     /inference="COORDINATES: protein motif:HMM:NF015413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIO9553281.1"
                     /translation="MDLLDRDLSKVQLSVTHKRLEELHPADIADILEQLDPQQRANVF
                     KTLDENQASEAVAEMEDEIQAEFLEDLDEHKASKVLGNMDPDDAAEIVRDLSYDKAEK
                     LLRLMGMEDATDIRRLLGYKDGTAGGMMTTQYVAVQEDDTVNDTIDVIRKLDEDHPPV
                     HYIYVLDKSKCLVGAVSLKALALNNGNTQMKNIMIHDDLIYVSPDEPEDEVSEDIFKY
                     DLTAMPVVDEHGVLLGIVTTEDAWDAIEDDVVEKKNFT"
     gene            1650..1793
                     /locus_tag="AC04VQ_03695"
     CDS             1650..1793
                     /locus_tag="AC04VQ_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553282.1"
                     /translation="MTKFKKNKFLLVLAAMGPGIITAMAGNDAGGIATYSTAGANYGF
                     ATL"
     gene            1806..2888
                     /locus_tag="AC04VQ_03700"
                     /pseudo
     CDS             1806..2888
                     /locus_tag="AC04VQ_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013715.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
     gene            2908..3393
                     /locus_tag="AC04VQ_03705"
     CDS             2908..3393
                     /locus_tag="AC04VQ_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIO9553283.1"
                     /translation="MKLKRILSTLLCVCLVASFCVVGVGCSSSVDKYKPVKKSTNEAG
                     KDIYEWPSGSETGMIDVPGGKVAYRVYGKDKKGTPLVCVHGGPGGNYSNFYKQISLAE
                     DRPVILYDQLGCPLSEVDESVYNTPDKVKSLYTIDRYTEELDCVIKYFELENFILYGT
                     S"
     gene            3409..3999
                     /locus_tag="AC04VQ_03710"
     CDS             3409..3999
                     /locus_tag="AC04VQ_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIO9553284.1"
                     /translation="MEYVGKYHPAGLKGMVFGGPFLDVDYWIADAQRLILSLDNGQEM
                     LNHVRLCEQTGNYDDKFKEINSVFSKNFNNRHPDCTYNKPDMTNGAPGHIKIQGVDVY
                     VYMWGESEFSCTGTLNHHDSSKFLADLNVPVIFMPGQYDEGTPEAAFHYASLCPKGIA
                     EVAVLPGAGHAASFERPEEFNAILSQFCFRIDNNES"
     gene            4078..4716
                     /locus_tag="AC04VQ_03715"
                     /pseudo
     CDS             4078..4716
                     /locus_tag="AC04VQ_03715"
                     /inference="COORDINATES: protein motif:HMM:NF015866.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
     gene            complement(5108..5416)
                     /locus_tag="AC04VQ_03720"
     CDS             complement(5108..5416)
                     /locus_tag="AC04VQ_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553285.1"
                     /translation="MTIAPGLLITVIAILFNLVIIILGICGVLSAGMVIAASLSSIVF
                     CLANYILFMFVLGVITIFVEWYKFRSKTGAKIGAMFTFPFFMLTYIPIAIIAVFKKVK
                     "
     gene            complement(5804..6397)
                     /locus_tag="AC04VQ_03725"
     CDS             complement(5804..6397)
                     /locus_tag="AC04VQ_03725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIO9553286.1"
                     /translation="MDVFNFCVFLTFTVCYAYQLFYVLVGLLRKVEHKPAKKNHKYAA
                     IISARNESAVIGNLLDSINNQDYPSELIDVFVIADNCTDNTAEIAMKHGAFVIERNNT
                     ELIGKGYALDYGMNIIFRDCLGKKIGKSVCGKASYGKIQAPKGRKIDPRSGYEAFFVF
                     DADNVLDKNYFAAMNRVYDKGFTVATSYRNTKNFDSN"
     gene            complement(6461..7123)
                     /locus_tag="AC04VQ_03730"
     CDS             complement(6461..7123)
                     /locus_tag="AC04VQ_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding metallopeptidase family protein"
                     /protein_id="HIO9553287.1"
                     /translation="MLAELVVGYHDAEKAARIAEDIIDMAKAKYSDGASLEYNIEIRE
                     ASDIPVLSISDSMKIYHLLTMLPIGIIETYDDGQVKTSNNVGLLEIYDDVLNCEILFR
                     STDPEQLKRAVRTILDVSLDNDIDFMIETIFPEWPKVENNPLEKLIGEAYAKTSNLEV
                     DTLDIHAGLENGYLLRKKPDLIMTSIGCDIVNEHSRKETLFTKSLPAYCAAILYTLKH
                     LS"
     gene            complement(7283..7567)
                     /locus_tag="AC04VQ_03735"
                     /pseudo
     CDS             complement(7283..7567)
                     /locus_tag="AC04VQ_03735"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7598..7765)
                     /locus_tag="AC04VQ_03740"
     CDS             complement(7598..7765)
                     /locus_tag="AC04VQ_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553288.1"
                     /translation="MDIPATKGYEDTPKLMFQAHFDMVAVASKENHDFDPKTSPIVPI
                     VDDKEGVIHTG"
     gene            complement(7817..7999)
                     /locus_tag="AC04VQ_03745"
     CDS             complement(7817..7999)
                     /locus_tag="AC04VQ_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553289.1"
                     /translation="MKKIKKMTYPNKIAEKATVSKEYSKTANIMTKNLIDEFLPLCDI
                     PHPSGHVDEMRQYLLD"
     gene            8051..8497
                     /locus_tag="AC04VQ_03750"
     CDS             8051..8497
                     /locus_tag="AC04VQ_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIO9553290.1"
                     /translation="MKKTKKVNLSRHKFYTLFTAGTISFAVSTIAGFLDNIVSGNFLG
                     EAAISAVGLVTPIFTIVCFISTLFANGAANYYTEAVGEFDKKKAYEIFGTAIIASIAL
                     GVIMMVSLFFLEDAIINWFGVGEEISDYCHQYFKYFILLSLIQPMD"
     gene            8498..8989
                     /locus_tag="AC04VQ_03755"
     CDS             8498..8989
                     /locus_tag="AC04VQ_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553291.1"
                     /translation="MLFAIVYNDGDQNCTIVSDVFKVGTNIIFAIIFCSLMGVAGVSL
                     ARVISTVIAMIILCCHFFRKQNSLKVRFVFKTKYCPTIVKYSAVIAFGYLFTAILNIA
                     MNEFVVEMFGEFYIPVVNVIMFVFSIITVCSSNGTAITPFVCVYLSEKNMPGLKNIMN
                     AGL"
     gene            9341..>9774
                     /locus_tag="AC04VQ_03760"
     CDS             9341..>9774
                     /locus_tag="AC04VQ_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553292.1"
                     /translation="MGGKKKFPLLIDDFGTNTKNYNFVLSAEAFAEVNKEIVDFLKEN
                     NIANKSVQKASLLIEEMGVKIQDFNKDHKGKILIEISVQLDPQHPGKVFIVVRDNGKF
                     LDVTDEDSAVTSLNSYMLASYMRAVQDKTYVKTIGFNRSHYE"
BASE COUNT         2782 a         1817 c         2130 g         3045 t
ORIGIN      
        1 gcacagacag gtgatgaaaa tggcatgttg attgcaattt taattgctgt tgtggcgctt
       61 gcgattgttg ggcttgccag ctacttcgtt attaataaag tcaagaataa caaagcgaag
      121 tcaaaacacc ccaagcatta attttaattt aaaagttaaa agaccgcaaa attatcaaaa
      181 atcggtattt ttgcagtatt ttggggtaaa atacgtggtc cgcgaaaaat tgcggaagtt
      241 ttgagatgaa tagaaaggat ttttacatgt tgattggagg tgacgcatat gatatacctc
      301 tcccgcttga ttggaaagcc agttgtggac tgcgaaggca agcgccttgg gagcgtatcg
      361 gacgtcgcca tttcaacagg tgaagtcttt cctaggataa ccagtcttgc tttcttagga
      421 cctggtaaaa caccatttat gatttcatgg cgcaagttcg tcgaagaatt tgacgaagac
      481 gacggcatta agttgaagtt gccgcagcct cagattcgtt tttcttattt gcaacctgac
      541 gaagttttgc tcgcgcgtga tttgctcaac aggcaaattg ttgatacgca gggcttaaag
      601 gttgttcgag ttaatgattt gaagctttcg caatatggaa atcagcttcg tttgcttggt
      661 gcggaagttg gaattcgtgg tcttttgcgt gggttggctg gttgacttga aaagcttgtt
      721 gttggaattt cgaagctggt caaacaccca attcaagagc aaattattgc ctggaattat
      781 atggatttgc ttgaccgaga tttgtcgaag gtccagctat ccgttactca taagcgcctc
      841 gaagagctgc acccagcgga tattgctgac attttggagc agcttgaccc tcaacagcgc
      901 gcgaatgttt ttaaaacgct ggatgaaaat caggcttccg aagcagttgc ggaaatggaa
      961 gatgaaatcc aggctgaatt tttggaagat ttggatgaac acaaggcttc gaaagtttta
     1021 ggaaacatgg atccagatga cgcggcggaa attgttcgtg acctgtctta tgacaaggct
     1081 gaaaaattgc ttcgcttgat gggtatggaa gacgcgacgg atattcgtcg acttttgggt
     1141 tataaggatg gcactgctgg cggtatgatg acaactcaat atgttgcggt tcaagaggac
     1201 gacaccgtca acgacacgat tgatgttatt cgtaagcttg acgaagacca tccgccggtt
     1261 cattatattt atgtgttgga caagtcaaaa tgcctggttg gagctgtgtc tctcaaagct
     1321 ttggcattga acaatggcaa cactcaaatg aaaaacatca tgattcacga tgatctaatt
     1381 tacgtttccc cggatgagcc cgaagacgaa gtttccgagg acatctttaa atacgatttg
     1441 actgcaatgc cagttgtgga tgaacacggg gtgctccttg gcatcgtaac taccgaagat
     1501 gcttgggatg caattgaaga tgatgttgtt gaaaagaaaa actttacttg agcaaaacgc
     1561 attgggctaa ttttgttggg tctcattttg ctcgctcttt atacattggt aattatttat
     1621 tttgtgaaag ggttttaaat ggggccggca tgacaaaatt caagaaaaat aaattccttt
     1681 tagttttagc agcaatgggg cctggcatta ttactgctat ggccggaaac gacgctggtg
     1741 gaattgcaac ttattcgacc gctggcgcaa attatggttt tgcaactctt tgaacaattc
     1801 ctataatgtg cgttttgctt atcattgctc aaggcacggc ttcgcgaatg ggcgctgtca
     1861 ccggtaaagg ttttgctgca ctgattcgcg agcgcttcgg aattcgatgg accgcgtttg
     1921 cgatgttggt cctgctgatc ggcaacactt ccacaacttt cagtgagttc gccggaattg
     1981 catccggcat ggagctcttc ggagtgtcga agtatatttc tgtgcctgtt gcggctgtgg
     2041 ccgtttggtt tttgatcgtt ggtggaaatt ataagcgtgt cgagaaggtt ttccttgcca
     2101 ttagctgcgt ttttgtcacc tacatcattg cggcttttat gagtggtccc gactgaatgc
     2161 agactataag tagcactttc attccaacaa ttttgccgga ccaaaacttc attagcgtaa
     2221 ttattgcgat gattggtacc acaattgcac cgtggatgct cttcttcgtt caaagcaaca
     2281 ttgtcgaaaa aggtaacggt gcaaaaaaga ctgaacttga aaaggatttg ttcagcggtc
     2341 gagtggatgc aatttctgga tcggtcgcgg cattggcagt tgcttggttt atcatcgtta
     2401 caaccggaag cgttttgttt ccgcaaggaa ttcaagttca aaccgccgaa gacgcagctc
     2461 gcgccttggc tccattcgct ggcgattacg ctcgaatctt gtttggaatt ggcctggtag
     2521 cagcttcgtt tttggcagca tgtgtattgc cgcttacaac ttcgtttgta gtttgcgaag
     2581 cgtttggttg ggaagctggc gttaacttta aatggaaaga agcaccaact tttaaaacat
     2641 tactcaccgt aattattgtt gcttcgatga ttgttgtcct gcttccagac attgggctta
     2701 tggacattat gcttcaatcg caatttatca gtggcctaat cttgccagtg cttttggtat
     2761 ttatggcgct tatcgctagc aacgaacgca tcatgggcaa atacgcaatc ggcccaatca
     2821 caaaattttt actttggata acagttataa ttgtatcgtg cttaacaatc gcattattta
     2881 ttgtatagag agttcagagg ttttattatg aaattaaaaa gaattttatc tacattatta
     2941 tgtgtttgct tggttgcaag cttttgtgtt gttggagttg gttgctcgtc ttcggtagac
     3001 aaatataagc cggtaaaaaa atcaactaac gaagctggca aagacattta tgaatggcca
     3061 tcgggttcgg aaactggaat gattgatgtt ccaggtggaa aagttgctta tcgagtttac
     3121 ggcaaagaca aaaaaggcac cccgcttgtt tgtgtccatg gcggaccagg tggaaattac
     3181 agcaattttt ataagcaaat ttctttggct gaagatagac ctgtaatttt atatgaccaa
     3241 ttgggctgtc cgttgtccga agttgacgaa agcgtttaca acacgcctga taaggttaaa
     3301 tctttataca cgatagatag atacaccgaa gaactagact gcgtcattaa atatttcgaa
     3361 ttggaaaact ttattttata tggcacatct tgaggttgca tgctaaatat ggaatatgtt
     3421 gggaagtatc atcctgcagg actcaaaggg atggtttttg gaggtccgtt tttagatgtt
     3481 gattattgga ttgctgatgc gcaacgctta attcttagtc ttgataatgg tcaagagatg
     3541 ctaaaccatg ttcgtctttg tgaacaaact ggtaattatg acgataagtt taaagaaatt
     3601 aactcggtct tttcgaaaaa tttcaataac cgacatcccg attgtactta taacaaaccg
     3661 gacatgacta atggtgctcc gggacatatt aaaattcagg gcgtagatgt ttatgtatat
     3721 atgtggggtg aaagcgaatt ttcttgtact ggaacgttga accatcatga ttcttcaaag
     3781 tttttggccg acttaaatgt ccctgtgatt tttatgccag gtcaatatga cgaaggcacc
     3841 cctgaagccg cgttccacta tgcttcgttg tgtccaaagg gaattgccga agtcgcagtg
     3901 ttgcctggtg caggtcacgc cgcgagcttc gaacgccccg aagaatttaa tgcaattttg
     3961 tcgcaatttt gcttccgaat tgataataac gaaagttaat aaatattatt aaacaaaatt
     4021 aaaccgggaa taaaccctcg ctgcccccgg ccccaagaaa ccaagaaagg attagatatg
     4081 agtgttttag ttgaagcttg ttgtgcatct gtagaagatg ttttaacggc cgagaaggct
     4141 ggatgcgatc gcgtcgagct ttgctcggca atgacccttg gtggtcttac accaagcgta
     4201 ggtatggttc gtgaaattaa gaagcattca aagataccta tcatggcaat gaatcgcccg
     4261 cgtagcggag gcttttgtta caccgatgct gaatttgaaa tgatgaaaga agatgcaagc
     4321 tctttgatcg ccgaaggcgt ggaaggcctc gtcttcggaa ttttacgcga ggatggcacg
     4381 gtcgacagtg aacgatgcgg cgaaatgatg cgccacatct cccatgagtc tatgaagtta
     4441 cccaaacgcg tcgaaactgt cttccataga gcaatagacg taactccgga ttgacgcgca
     4501 gccatcgaag agctaattgc aatgagcttc gacagagttt taaccagcgg ccaagtcccg
     4561 aatgccaatg aaggcgcaca aacaattgca gaaatggtca aacttgcagg cgacaggatt
     4621 gaagttatgc ctggtagcgg cgtcagccgc gaagcggctt caaatcttgt taaaactaca
     4681 ggcgtcagcc aaatacacct gcgagcagtg aagtgagttg acgacaattc ttgtaagaat
     4741 ggcccaaatg aaatcccatt caacggaact ggggacccag aaactcaatt cgcaatttca
     4801 gacaaagaat acttccaaca actaattagt aatgtacgat aaatatttga gttgtcggct
     4861 cagacgggga gattttggaa tatatttttt gcgaactcgc aagagagcga ggagagatcc
     4921 actgagtaaa attgatgtca attagttttt tggattagga attcaaaaaa cgtcaattga
     4981 caacaaattt tatgaagtta gctcgacgag cgggcggtga gcaaaaagtg tgttccaaaa
     5041 tctcttacct cttcgcctct tcggcgaatt ctttaggatc aactgcgata gtgtgtttga
     5101 taggcgttca cttgactttc ttgaacactg caataatcgc aattggaata taagttaaca
     5161 tgaagaacgg gaaggtgaac atggcaccaa tcttcgcgcc agtcttcgag cggaatttgt
     5221 accactcaac gaagatagta atcacgccaa gcacaaacat gaaaaggatg taattcgcaa
     5281 gacagaacac gatggaagac aggctggccg cgatgaccat cccagcacta agcacaccac
     5341 agatgccaag tataataatc acaagattga aaagtatcgc aatcacagtg atcaaaagtc
     5401 caggcgcgat ggtcataagc atgtcatagc acgcgaagcg gtaccccttc ggattcgtga
     5461 agatgtgttt tacaagttgc ccgctgtatt taacgaagac ttgataaaag cctcgggttc
     5521 atctaaacct ttgattccaa gaatctttga atgtgacagg ttgctcatcg tagagaacag
     5581 catcgggagc atagccgatt cggttagcgt gaatgatgtt gttggtcgca aattcgatat
     5641 cttcggtaag aaggaatcac ttccacccgc cagctttttc aataattttg acatcagtga
     5701 aaaatccggt gcccgaaaca gcacaacttg tgttcaagta agcgcgaggt tgcgaaagga
     5761 agcgcgcctc gcgaataaat catgttccat aacccgaaga aattcaattg ctgtcaaagt
     5821 ttttggtgtt tctgtaactt gttgcgactg taaaaccctt gtcataaacc ctgttcatag
     5881 cagcaaaata atttttgtct aaaacattgt cagcatcaaa gacgaagaaa gcctcgtaac
     5941 ccgaacgtgg atcaatcttg cgaccttttg gagcttgaat ttttccataa cttgctttac
     6001 cgcaaacact cttgccaatt tttttgccaa gacaatcgcg gaagattata ttcatcccat
     6061 aatcaagagc ataacccttg ccaattagtt cggtattatt tcgctcaata acaaaggcgc
     6121 cgtgtttcat cgcaatttca gcagtgttgt ccgtgcaatt atctgcaatt acaaaaacat
     6181 caataagttc gcttggataa tcttgattat ttattgaatc aagcaagttg ccaattacag
     6241 cactttcatt tcgcgcactt ataatggcag catacttgtg gtttttcttc gctggtttat
     6301 gttcaacttt acgaagcaaa ccaacaagaa cataaaataa ctgatacgca taacagactg
     6361 taaatgttaa aaaaacgcag aagttaaaaa catccacgaa tgatatgtat gacaacatgc
     6421 caactatcgt ttgcatagcg aggtggaatt ataccatata ttaggataaa tgcttaagtg
     6481 tgtatagaat tgcagcgcaa taagcaggta gagactttgt aaacagagtt tccttgcgac
     6541 tatgttcatt gactatgtca cagccaatac ttgtcataat taagtctggt tttttacgaa
     6601 gcaagtatcc attttcaaga ccagcatgaa tatcaagtgt atcaacttcc aaatttgaag
     6661 tcttggcata agcttcgcca atcaattttt caagcggatt gttttcgacc ttcggccatt
     6721 caggaaagat tgtttcaatc ataaaatcaa tatcattatc aagcgaaaca tccaagatgg
     6781 tgcgaaccgc acgttttagt tgttcaggat cggttgagcg gaagagaatt tcgcaattta
     6841 gcacatcgtc ataaatttca aggagcccaa cgttgtttga agtttttact tgaccatcat
     6901 cgtatgtttc aatgatgcca attggaagca ttgtcaaaag atgataaatc ttcatggaat
     6961 cgctaatgct taaaactggg atgtcgctgg cttcgcggat ttcgatgttg tattcaagcg
     7021 aagcgccgtc gctgtatttc gcttttgcca tatcaataat atcttcagca atgcgtgcgg
     7081 ctttttcggc gtcgtgatat ccaacaacca attccgccaa catttttgaa ggaatagcat
     7141 tcattaaaat tccgcttttt atataacgaa tattgaagtt gatgctttcg gccaacaaac
     7201 attcaagata tgcggttaaa attgcaagtg tgttacctcg gtttttatct atgtcagcac
     7261 cagagtggcc tccattaaga cctcaaattt caaacgaaat aacgtttgac tttttgcccg
     7321 gagccattct gtccattttt ttacgagttg ttgcataaaa tccaccagct gccgaagtaa
     7381 ttatcattcc gacatatatt gaatcaagat taattacata attagaatca acaacatctt
     7441 tgtttaaaag tttaacgccc ttaagtgttg tttcttcatc atatgtaaac aacagacgaa
     7501 ttggtccatg ttcaaattca gctttgtctt catgcatttt tgcaatagca agcatagttg
     7561 caatgccatg tccatcatct gcgccaagcg aagtttttca acctgtatgt attacgcctt
     7621 ctttatcatc cacaattgga acgataggag aagtttttgg atcgaagtca tgattttctt
     7681 ttgaagcaac cgcaaccata tcgaagtgag cttgaaacat caacttgggc gtgtcttcgt
     7741 aaccttttgt tgcaggaatg tccatataaa tacatcccga actatcaagt tgagtttcaa
     7801 tattatggga ttttcctcaa tcgagaaggt attgacgcat ctcatcaaca tgaccagaag
     7861 ggtgtggaat atcacaaagg ggcaaaaatt catcaattaa atttttagtc atgatgtttg
     7921 cagtcttaga atattccttg ctaacagtgg ctttttctgc aattttgttc ggatatgtca
     7981 tttttttaat ttttttcacg gcgcacatta taacacttag ttttttcaat actttgatat
     8041 aatcccaggt atgaaaaaaa ccaaaaaggt aaatttaagc agacataaat tttatacgct
     8101 attcacagcg ggaacaattt cttttgctgt ttctactatt gctggttttt tagataatat
     8161 tgtttcagga aattttcttg gcgaagctgc aattagtgcg gttggtttgg ttactccaat
     8221 ttttacaatt gtatgcttta tttcaacgct ttttgcaaat ggagctgcta attattacac
     8281 tgaagctgtt ggtgaatttg acaagaaaaa agcttatgaa attttcggca ctgcaattat
     8341 tgcttcaatt gctcttggag tgatcatgat ggtgtctttg ttctttttag aggatgccat
     8401 aattaattgg tttggtgtag gcgaagaaat ttctgattac tgtcatcaat actttaaata
     8461 ttttattttg ctttcactta tccagccaat ggattgattg ctgtttgcta ttgtttataa
     8521 cgatggtgac caaaactgta ccattgtttc tgatgttttc aaggttggaa ctaatattat
     8581 ctttgcaatt attttctgta gtttgatggg tgttgcaggt gtttcgcttg ctcgcgtaat
     8641 tagcacggta attgctatga taattttgtg ttgtcatttc ttcagaaagc aaaattcgct
     8701 caaggttcgc ttcgtcttta aaacgaagta ttgtccaacc attgttaaat atagcgcagt
     8761 tattgctttt ggttatttgt ttactgcaat tttaaatatt gctatgaatg aatttgttgt
     8821 tgaaatgttt ggcgaatttt atattcctgt tgtaaatgta atcatgtttg tatttagcat
     8881 tattactgtt tgctcttcta atggaacagc cattacgcct tttgtttgtg tttatttaag
     8941 cgaaaagaat atgccgggcc taaagaatat tatgaatgca ggtttgtgag ttgcaattat
     9001 cgaaggtgtt gtcatcactt tactatttat gatttttgga gattttgtca gcgttattta
     9061 tggaatcaca actcctgctc ttgttgatat ggcaagtgtg gcaattagat gagcttcggt
     9121 gtgcattgta tttgcctcca ttatttattt atatgttcaa tattatgttg ttgcaggaca
     9181 cgcggtctta agcgttgttt tttctggctt gtttatgacc gttgctccaa tttgaagact
     9241 ttatttattt ggctatttat gaggtcagat tccaggatga gccggttgag gtctttcctt
     9301 tggtcttgct tttataattt gtgttattgt agttttcatt atgggcggga agaaaaagtt
     9361 ccctcttctt attgatgatt ttggcactaa cacgaaaaac tacaattttg ttttaagtgc
     9421 tgaagctttc gctgaagtca ataaagagat tgttgacttt ctcaaagaaa ataatattgc
     9481 gaacaaatct gttcaaaaag caagtttgtt aatcgaagag atgggtgtta agattcaaga
     9541 ctttaataaa gatcacaaag gtaagatttt aattgaaatt agcgttcaac ttgacccaca
     9601 acatccaggt aaagttttca ttgttgttcg tgacaacggt aagtttttag atgttactga
     9661 cgaagacagc gccgtaacca gcttgaactc ttatatgtta gcaagttaca tgagagctgt
     9721 tcaagataaa acctacgtaa aaaccatcgg ctttaaccgc tcccactacg aaat
//
LOCUS       DBNCDC010000035         9762 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_2997_length_9762_cov_8.702380, whole genome shotgun sequence.
ACCESSION   DBNCDC010000035 DBNCDC010000000
VERSION     DBNCDC010000035.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 9762)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9762)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9762
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2997_length_9762_cov_8.702380"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="AC04VQ_03765"
     CDS             <1..293
                     /locus_tag="AC04VQ_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013543.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIO9553293.1"
                     /translation="SIYPTLGVILALVVLVTLVSGATDAPNAIATAVSTRCMKVGTAL
                     GIAAVANFVGLIAMTYVTTAVAETMFNMVNFGSSDAHISLMALMAAMIASIG"
     gene            411..686
                     /locus_tag="AC04VQ_03770"
     CDS             411..686
                     /locus_tag="AC04VQ_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553294.1"
                     /translation="MKVIYGMVFSLVAGFILGWVLVRIIEIFFKGVSRRQGNLVCKYV
                     QNVLAVILAGLHGAQDGQKFMSIAILGMSLAVGSGSGLEMHIDSFPF"
     gene            690..959
                     /locus_tag="AC04VQ_03775"
     CDS             690..959
                     /locus_tag="AC04VQ_03775"
                     /inference="COORDINATES: protein motif:HMM:NF013543.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIO9553295.1"
                     /translation="MLLCSATMALGTLIGGKRIIKSVAMDMVSLEKYQGLASSLSTTL
                     MLLIATLTGMPVSTSHCNTSSIMGVGSAKNMKNVKWKVAGNMVLA"
     gene            1107..2864
                     /locus_tag="AC04VQ_03780"
     CDS             1107..2864
                     /locus_tag="AC04VQ_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIO9553296.1"
                     /translation="MGVGFFGGLQMTCGSMKGALTNYYNETNMMDIRIVCSLGIDQTY
                     IDDIKNIDEVEGVMPSYETDILTNFGNDQSAVRVHSLPDNLDINDCNYINQQILARGQ
                     WPKNADECVLSEDAVLASPPQIGDEIDILDCSTGLDNTLSVKKFKIVGFVHSSYYVGS
                     VQMGQSNLGKGNVNQFAYVLQSAFAKDFPFTEVFVTVKDVDKYVTGSAEYQAKVDEVM
                     TQLVDASPREVDIRSSHVRHNAQKQIDEAQTQIDSNQNLLDKKYQEFNAYRDYLLGLV
                     DDGIKTANATIDKTNNIKDQANTNFNNLEQANVQVENFETCMTGLLDIINKTNEAIQI
                     NNSLSNFVESTVVKQQIEELNAACNYFVEYSQKFYSSLQFAYSREVYDYYIGTFTTLY
                     EGLRSALPLRCVLCDTIVDKCNDVINNELLPQRNNIITKFNEQEGALIDGELQLSLAK
                     LKLQDSQSLLAELNAKILIMDRTKNIGAISFDNDADRIASIANVFPLIFFFVALLVAL
                     TSMTRMIEEERITIGTYKALGFSRFRISSKYILYGVISSFVGSAIGLLLLTQFLPYVI
                     MYAYSIIYKLPIPFPMPFN"
     gene            3066..4196
                     /locus_tag="AC04VQ_03785"
     CDS             3066..4196
                     /locus_tag="AC04VQ_03785"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIO9553297.1"
                     /translation="MFLYKKRLFMTLIGVAGCTALLLTGFGLHDSIGDIMVMQFDEIA
                     VDNLKVTFEDSASQADKDDVCRTINTINGQTASTLVHEENVVAMPQGHPNAPTVIVVP
                     NNVEQFQEMRVMRNRTSKEQYTLDDSGVYISEKLANLMHVNVGDSITVYNQDLIGNAG
                     DKEFSFTIAGIVENYIAHYIYMTPNLYKHYFENDCSYKNLIANTNLASDAQQGLIDNI
                     CTNKSVKAAYFTTSNRENFERMLKSVNMVVVILIVAAGLLAFVVLYNLININICERAR
                     EIATLKVLGSNKFETNMYINRETFVLSFLGSLIGLILGVIMEQFVILSAEVDMVMFGR
                     IIHLESYLISLAITLVFTVLVSLFMRKKLFSISMVESLKSVE"
     gene            4206..4904
                     /locus_tag="AC04VQ_03790"
     CDS             4206..4904
                     /locus_tag="AC04VQ_03790"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIO9553298.1"
                     /translation="MSFVEFKNVKKIYGSGEVEVRAANGLNFTMEEGEIIVVVGPSGS
                     GKTTLLNMLGGMDNVTSGKIILDGNEITKYNEKDLINYRRHDIGFVFQFYNLIQNLTA
                     LENVELANQLCDNALDAKKTLELVGLKKRMDNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDYKTGKEVLKVLQDTALKHGKTVVIITHNSAFCDIANRVIRIRGGKVIE
                     EKLNKKPVSAETLE"
     gene            4988..5956
                     /gene="fba"
                     /locus_tag="AC04VQ_03795"
     CDS             4988..5956
                     /gene="fba"
                     /locus_tag="AC04VQ_03795"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HIO9553299.1"
                     /translation="MSLVTSTEMFKKAYDGGYAIGAFNVNNMEIIQGITEAAGELNAP
                     LILQVSKGARAYANHTYLLKLVEAAVEENPNIPICLHLDHGDTFELCKDCIDGGFTSV
                     MIDASSKSFEDNIKLTKQVVEYAHDKGVVVEAELGTLAGVEDEVKVAAGDASYTKPEE
                     VEEFVEKTGCDSLAIAIGTSHGAFKFTPEQCTRDENGILVPPPLRFDVLEECIKRLPG
                     FPIVLHGSSSVPQEFVAEVNKYGGNMPDAIGIPEDQLREAAKLAVCKINIDSDIRLAI
                     TASVRKHFAEHPEHFDPREYLKPARQAVKDMVARKMINVLGCDGKA"
     gene            6024..6158
                     /locus_tag="AC04VQ_03800"
     CDS             6024..6158
                     /locus_tag="AC04VQ_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553300.1"
                     /translation="MKDKIMQGAQRFGGAMFTPVMLLAFSGMIIGIATLFTSSQIWGS
                     "
     gene            6189..6362
                     /locus_tag="AC04VQ_03805"
     CDS             6189..6362
                     /locus_tag="AC04VQ_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553301.1"
                     /translation="MWNLLAEGGWTIFNQMPLIFVVGLPIGLARKQHARCCLEALLLY
                     LTFHYFISTILLQ"
     gene            6372..6494
                     /locus_tag="AC04VQ_03810"
     CDS             6372..6494
                     /locus_tag="AC04VQ_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553302.1"
                     /translation="MGFDLETMDVSNDPRLADVAGLTTLNMGIAGSLLISGIVV"
     assembly_gap    6776..6875
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6876..7313
                     /locus_tag="AC04VQ_03815"
     CDS             <6876..7313
                     /locus_tag="AC04VQ_03815"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit EIIB"
                     /protein_id="HIO9553303.1"
                     /translation="TLIAIGLVYTFIYFIVFRFLILKFDYKTPGRDSDDAIKFYSKEQ
                     YKEKKEMEKEVKDADERAGIESKEESLEEKILFLVGGRENIIDVTNCATRLRLNVKSG
                     AKVADDKEFKAIGTHGAKITGNSVQVIIGLDVPNVREKFEALL"
     gene            7329..8660
                     /locus_tag="AC04VQ_03820"
     CDS             7329..8660
                     /locus_tag="AC04VQ_03820"
                     /inference="COORDINATES: protein motif:HMM:NF023401.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HIO9553304.1"
                     /translation="MSSEIKPQSIVIAGGGSTYTPGIVMMLLENLDRFSIRKLTFYDN
                     FAERQEIVAKACDICIKENAPEIDFSWTTDPKTAFTDCDFVMCHIRVGLFAMREQDEK
                     IPLKYGVIGQETCGPGGIAYGMRSIGPVIEILDYMEKYSPNAWMLNYSNPAAICAEAC
                     RRLRPDSKIINICDMPIDLGDRLADICGLESRKQLDIGYYGLNHFGWWHSIKDLDGND
                     LMPQIREHMAKFGFSQADKPKDDESWNDTYRKAADTFALDPTTIPNTYLQYYLYPDDM
                     VAHMDPNYTRANYCIDHREKDVFEACQKIIDSGTAKDCGFEADEHAMYIVDLSMAIAT
                     DSKEKMLLIVQNDGALDNFEPESMVEVPCIVGSNGYEIVPQGKIGQFQKGLMEQQVAV
                     EKLVVDAWINHSYQELWQALTLSRTVPSANIAKKILDDLIEANKDYWPELK"
     gene            8669..>9762
                     /locus_tag="AC04VQ_03825"
     CDS             8669..>9762
                     /locus_tag="AC04VQ_03825"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIO9553305.1"
                     /translation="MKNLIVGQSGGPTAVINSSLYGVVKTALDSNEIDKVYGMQNGIE
                     GFLKGTILDFEEALPGDKLELIKTTPGAYLGSCRYMLPDDLDDETYPKLFEKLEELEI
                     AYFLYNGGNDSMDTVSKISRYAERVGSDIKVLGVPKTIDNDLVMTDHTPGFPSAARYV
                     ANTVREIVTDASVYETPAVTIIEIMGRHAGWLTAASVLARRFEGDAPYLIYLPEVDFD
                     LDDFVKRVEALLKEHNAVVVCVSEGINDADGKFICEYDSATGVDSFGHKMLAGAGKFL
                     ENLLRDKLGCKARSVEFNVSQRCSSALMSKTDQEEAIMAGRFGVESALAGETGYMIAF
                     DRVSDYKIECSLQDVNKICNEEKKVPASMI"
BASE COUNT         2856 a         1432 c         2119 g         3255 t
ORIGIN      
        1 tgtcaattta tccaacgctg ggagtaatcc tggcgttggt tgttttggtt acgcttgtat
       61 ccggcgcgac cgatgctcca aatgcaattg caactgcggt atctaccaga tgcatgaagg
      121 taggcactgc acttggaatt gctgctgtgg cgaacttcgt tggtcttatt gctatgactt
      181 atgtcaccac tgcagtagcc gagacaatgt ttaacatggt aaattttgga tcgagcgatg
      241 ctcacatatc gttgatggcg ttgatggcgg caatgattgc ttcgattggg tgaggagcgt
      301 tttgttgatt tttgggtatt cctgcgtcaa aatcacattc tttgattgcc ggaattaccg
      361 gaggagccat tgctcttaat ggtatggatg gcgttataat tcctgagtga atgaaagtaa
      421 tttacggaat ggtttttagc cttgtagctg gttttatttt gggatgggtt cttgttcgca
      481 tcatcgaaat tttctttaaa ggagtgagcc gaagacaagg caacctcgtt tgcaaatatg
      541 tccaaaatgt tttagctgta attttggctg gacttcacgg cgcgcaagat ggccaaaagt
      601 ttatgtcaat tgcaatttta ggaatgtctt tagctgttgg ttctggcagt ggacttgaga
      661 tgcatattga ttcttttccg ttttgattaa tgcttctttg ttccgcaaca atggcacttg
      721 gaactttaat tggaggaaaa cgtattataa aatctgttgc tatggatatg gttagtttag
      781 aaaagtatca aggtcttgct tcgagtttaa gcacgacctt aatgctttta attgcaactt
      841 taaccggaat gccagtttct acttcgcatt gcaatacttc ttcaattatg ggagttggtt
      901 cagcgaagaa catgaaaaat gttaaatgga aagttgcggg aaatatggtc ttagcatgag
      961 tccttacatt cccttgttgt ggtttgattg gttggtgttt agccaaactc ttcatgcttt
     1021 tctagatgaa taatttttga aaagatttat ttcgaacgat tcgccacagt tttgggcgat
     1081 ttcttgcaat tgctggaatt tgtgcattgg gtgttggatt ctttggtggg ttgcaaatga
     1141 catgtggaag catgaaaggt gcgcttacca attactataa cgaaaccaac atgatggata
     1201 ttagaatcgt ttgtagttta ggaattgatc aaacttatat tgatgatata aaaaatattg
     1261 acgaagtcga aggcgtaatg ccttcctatg aaacagatat tttgacaaat tttggcaatg
     1321 atcagtctgc tgttcgtgtg cattcgcttc cagataatct tgatattaac gattgcaatt
     1381 atattaatca acaaattttg gcacgtgggc agtggccaaa aaatgctgat gagtgtgttc
     1441 tttcagaaga tgctgtttta gcttcgcctc ctcaaattgg cgatgaaatt gacattttag
     1501 attgtagtac tggtctagat aatacattaa gtgtcaaaaa atttaagatt gttggatttg
     1561 ttcattcttc atattatgtt ggtagtgttc aaatgggaca atcaaattta ggcaaaggaa
     1621 atgttaatca atttgcttat gttctgcaat ctgcttttgc caaagatttt cctttcacag
     1681 aggtttttgt tactgttaag gatgttgaca aatacgtaac tggttctgct gaatatcaag
     1741 ctaaagttga cgaagtaatg acgcaactgg ttgatgcttc gcctcgtgaa gttgatatta
     1801 gatcttctca tgtaaggcac aatgctcaaa agcaaattga cgaagctcag acacaaattg
     1861 attcaaacca aaatttgttg gataaaaaat atcaagaatt taatgcatat agagattatc
     1921 ttttaggctt ggttgatgat ggtattaaaa cagctaatgc aactatagac aaaactaata
     1981 atataaaaga tcaggcaaat actaatttta ataatcttga acaagcaaat gttcaagttg
     2041 aaaactttga aacttgtatg acaggtctat tagatattat taataaaact aacgaagcaa
     2101 ttcaaatcaa taattctttg tcaaattttg ttgaaagcac agttgttaaa cagcaaattg
     2161 aagagttgaa tgcggcttgt aattattttg tagaatactc gcaaaagttt tattcttcat
     2221 tgcaatttgc ttattctcga gaggtttatg attattatat tggaacgttc actacattgt
     2281 atgagggttt gagaagtgcg cttcctcttc gttgtgttct ttgtgacact attgttgaca
     2341 aatgtaatga tgtaatcaat aatgagcttt tgcctcagcg aaataatatt attacaaaat
     2401 ttaatgagca agaaggtgct ttaattgatg gcgaattaca gctaagtctt gctaaattga
     2461 aattacaaga ttcgcaaagt ctgcttgctg aacttaatgc aaaaatctta atcatggaca
     2521 ggactaaaaa cataggggca attagttttg ataatgatgc agatagaatt gcttcaattg
     2581 caaatgtctt tccactaata tttttctttg ttgcactttt ggttgcatta acttcgatga
     2641 ccaggatgat cgaagaagag cgaattacaa ttggaactta taaagcgctt gggttttcaa
     2701 gatttagaat ttcaagcaag tacattttgt atggagtaat ttctagtttt gttggtagtg
     2761 caatagggct cttgttgtta acccaatttt tgccttatgt aattatgtat gcttatagca
     2821 ttatttacaa gttgccaata ccgtttccta tgccatttaa ttgaacaatc ggcactattt
     2881 ctattttagc aggtgtattg ttgacagtcg tagcaacact tatggctgct atcaaaagtt
     2941 taagattggt gccagccaga ttgatgattc caactgctcc taaagccgga aaaagaatat
     3001 tccttgaata tataaaacct ctttgaaaaa ttttgtcatt taattgaaag attactttta
     3061 gaaatatgtt cttgtataag aagagattgt ttatgacatt gattggtgtt gcgggctgta
     3121 cagctcttct cttaacggga tttggtcttc atgattccat tggagatatt atggttatgc
     3181 aatttgatga aattgcagtt gataatttaa aagttacttt tgaagattcc gcttcgcagg
     3241 ctgacaagga tgatgtttgt agaacaatta atacgattaa tggccagacc gcttcgactt
     3301 tagttcatga agaaaatgta gttgcaatgc ctcagggcca tcccaatgct ccaacagtaa
     3361 ttgttgtgcc aaataatgtt gagcaatttc aagaaatgcg tgtcatgaga aatcgaactt
     3421 caaaagaaca atatactctt gacgatagcg gtgtttacat tagcgagaaa cttgcgaacc
     3481 taatgcatgt taacgttggt gatagcataa ccgtttacaa tcaagaccta attggtaatg
     3541 caggtgataa agaattcagt tttacaattg ccggtattgt tgagaattat attgctcact
     3601 atatttacat gactccaaat ttatataagc attattttga aaatgattgt agttataaaa
     3661 acttgattgc gaatactaat ttggcgagtg atgctcagca gggcttaatt gataatattt
     3721 gcacaaataa atctgttaaa gcagcttact ttactacttc aaatagggaa aactttgaaa
     3781 gaatgcttaa aagtgtaaat atggttgtgg taattttgat tgttgcagct ggtcttttgg
     3841 cattcgtagt tttatataat ttaattaata ttaatatctg tgagcgggca agagaaattg
     3901 caacacttaa agttttaggt tctaacaagt ttgaaacaaa tatgtatatt aatcgtgaga
     3961 cttttgtttt aagcttcctt ggctctttaa taggtttaat tcttggtgtt attatggaac
     4021 agtttgtaat tttgagtgca gaagttgata tggttatgtt tggaagaata attcaccttg
     4081 aatcgtattt aatttcattg gctattactc ttgtgtttac agttttggtt tcactattta
     4141 tgcgcaaaaa actgttctcg atttcgatgg ttgaaagttt gaaatctgtc gaataggagg
     4201 ttgttatgag ttttgttgaa tttaaaaacg taaagaaaat ttatggtagt ggcgaagttg
     4261 aggttcgcgc tgccaatgga ttaaatttta caatggaaga aggcgagatt attgtggttg
     4321 ttggtccttc gggttcagga aagacaactc ttttaaatat gcttggtgga atggataatg
     4381 tgacttctgg aaaaattatt cttgatggaa atgaaattac taaatacaac gaaaaggatc
     4441 ttataaatta tagaagacat gatattgggt ttgtatttca gttctataat ctaatacaaa
     4501 acttaactgc tttagaaaat gttgagcttg caaatcagct ttgtgataac gcgttggatg
     4561 ctaagaagac tcttgaactt gttggactta aaaaacgaat ggataacttt ccttcgcaac
     4621 tttctggtgg tgaacaacaa cgtgtctcaa tagcacgcgc tttagctaaa aatccaaaac
     4681 ttttactttg tgatgagcca acaggtgctc ttgattataa aacgggtaag gaagttttaa
     4741 aggttttgca agacactgcc ttaaagcatg ggaaaactgt tgtaattatt actcacaatt
     4801 cagctttttg cgatattgct aatagagtga tccgcattcg tggtggtaaa gtcatcgaag
     4861 aaaaacttaa taaaaaacca gtttctgccg aaactttaga gtgataagtt tttacttttt
     4921 gatataattt ctccctgtta aaaacgtgct tttatcacgc aaaattaaat agtgaaagga
     4981 agacattatg agtttagtta catctactga aatgtttaag aaagcttatg atggcggata
     5041 tgctattggt gctttcaacg taaacaacat ggagattatt cagggtatta ccgaggctgc
     5101 tggcgagctt aatgcccctc taatcctaca ggttagtaag ggtgctcgtg cttatgcaaa
     5161 tcatacttat cttttgaagt tggttgaggc tgcagttgag gaaaatccta acatcccaat
     5221 ttgtttgcac ttagatcatg gtgatacttt tgagctttgt aaagattgca ttgatggcgg
     5281 tttcacttcg gtcatgattg atgcttcttc gaagtcattt gaagacaaca ttaaacttac
     5341 aaagcaagtt gttgaatatg ctcacgataa aggcgttgtt gttgaagctg agcttggaac
     5401 tttagctggt gtcgaagacg aagttaaggt tgctgctgga gacgctagct acacaaagcc
     5461 tgaagaagtt gaggaatttg ttgagaaaac aggttgcgat tcattggcaa ttgctattgg
     5521 aacaagccat ggtgctttta agttcactcc agaacagtgc actcgtgacg agaatggaat
     5581 tttggttcca cctccacttc gttttgatgt tttggaagaa tgcattaaac gtcttcctgg
     5641 ttttcctatt gttttgcatg gttcttcttc agttccacaa gaatttgtag ctgaagttaa
     5701 taaatatggt ggaaatatgc ctgatgctat tggtattcct gaagatcagc ttcgcgaagc
     5761 tgcgaagttg gctgtttgca agattaatat tgatagtgat attcgtcttg ctattactgc
     5821 ttcggttcgt aagcactttg cggaacatcc tgagcatttt gacccgcgcg aatatttaaa
     5881 gccagcgcgt caagctgtta aggatatggt tgctcgcaag atgattaatg ttttgggttg
     5941 cgacggtaag gcataagtct tctataacta aaattaaggg ctcgaaaatc gggccctttt
     6001 ttatttgaga ttaggggttt attatgaaag ataaaataat gcaaggagca caacgttttg
     6061 gtggagcaat gtttactcca gttatgctgc tcgctttttc cggaatgatt attggtattg
     6121 caaccttgtt tacaagttct caaatttggg gttcgtgagc atcggatgag cagagtttcg
     6181 cttataagat gtggaatttg cttgccgaag gtggttggac aatttttaat caaatgcctt
     6241 tgatttttgt tgttggtcta cctattggtt tggcaagaaa gcagcatgct cgttgttgtc
     6301 ttgaagcgct acttttatat ttaacatttc attattttat tagtacaatt ttgcttcaat
     6361 gaggcgattt tttgggtttt gatttagaaa ctatggatgt atcaaacgac ccacgccttg
     6421 ctgatgttgc aggtttaaca actcttaata tgggtattgc aggatcgctt ttaatatcag
     6481 gaattgtagt ttgagttcat aatagatttt ttgatacaaa agttccaatt tggctaagcg
     6541 cttttaaggg ctccccactt atttgtatta ttggattctt cctactaatt ccagttgcag
     6601 tattaactgt ttttatttgg ccaattattc aggaaggcat ttctgctgtt tcgttatgaa
     6661 ttacaagcat aaaagaatac ggagtatttg tgtttggttt cttggagaag gcgttgctac
     6721 catttggttt gcatcatcta atttatttcc cattccaata cgataacttg gtagtnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacttt gatagctata ggtttggtat
     6901 atacatttat ttattttata gtcttccgtt tcttaatttt gaagtttgac tacaagacac
     6961 caggacgcga ttctgatgat gcaatcaaat tctattctaa agagcaatat aaagaaaaga
     7021 aggaaatgga gaaggaagtt aaggacgcag atgagcgagc aggaatcgaa tctaaagaag
     7081 aatctttaga agagaagata ttgttcttgg ttggtggtcg tgaaaatatt attgatgtaa
     7141 cgaattgtgc gacgcgtctt cgcttgaatg ttaagagtgg agcgaaggtt gcagatgata
     7201 aagaatttaa agcaattgga actcacggtg caaagattac cggcaacagc gttcaagtaa
     7261 ttattggtct tgatgttcca aatgttcgtg aaaagtttga ggctttgttg tagttgatgg
     7321 aggtttttat gagttctgag attaagccac aatctattgt tatagcaggt ggaggaagta
     7381 cttacacgcc tggaattgtt atgatgctgc ttgaaaattt ggacaggttt tcaattcgca
     7441 agttaacttt ttatgataat tttgccgagc gacaagaaat tgttgcgaag gcatgtgata
     7501 tttgtattaa agaaaatgct ccagagatag atttttcatg gacaactgat ccgaagactg
     7561 cttttacgga ttgcgatttt gtaatgtgcc acattcgtgt aggtcttttt gcaatgcgcg
     7621 agcaggatga aaaaattcct cttaagtatg gcgtaattgg tcaagaaact tgcggtccag
     7681 gaggcattgc atatggtatg cgaagcattg gtccggtcat tgagatttta gattacatgg
     7741 agaagtattc gcctaatgct tggatgctta actattcaaa tccagctgca atttgcgcgg
     7801 aggcttgcag gcgtcttcgt cccgattcaa aaattataaa tatttgtgat atgccaattg
     7861 atttgggaga taggcttgct gatatttgtg ggctggagtc tcgaaagcaa cttgatattg
     7921 gttactatgg attgaatcat tttggttggt ggcactcaat taaagattta gatggcaatg
     7981 atttgatgcc tcagattcgc gagcacatgg cgaagttcgg gttttcgcaa gctgataaac
     8041 caaaagatga tgaaagttgg aacgatactt atagaaaggc tgcagatacc ttcgctcttg
     8101 accctacaac aattccaaat acatatttgc aatattattt gtatccggat gacatggttg
     8161 ctcatatgga tccaaattat actcgtgcaa attattgtat tgaccacaga gagaaagatg
     8221 tttttgaagc ttgtcaaaaa attattgata gtgggactgc taaagattgc ggttttgaag
     8281 ctgatgaaca tgctatgtat attgttgatt tatcaatggc aattgcaact gattcaaaag
     8341 aaaagatgtt gttaattgtt caaaacgatg gtgcacttga taattttgaa ccagaaagca
     8401 tggttgaagt tccttgcatc gttggcagta atggttatga aattgttcca caaggaaaaa
     8461 ttggacaatt tcaaaaaggc cttatggaac aacaagttgc tgttgagaaa ttagttgtgg
     8521 atgcgtggat taatcatagc tatcaagaat tatggcaagc gttgactttg tctcgaacag
     8581 tcccaagcgc aaacatcgct aagaaaattc ttgatgactt aattgaagca aataaagatt
     8641 actggcctga acttaaatag gaggtagtat gaaaaattta atagttggac aaagtggtgg
     8701 acctactgca gttattaatt caagtcttta tggtgttgtt aaaactgcgt tagattcaaa
     8761 cgagattgat aaagtctatg gaatgcaaaa cggcatcgaa ggatttttga aaggaacaat
     8821 ccttgatttt gaagaggcct taccaggaga taaacttgaa cttataaaaa ctactccagg
     8881 tgcttattta ggttcatgta gatatatgtt gccagatgac ttagatgatg aaacttaccc
     8941 taagcttttt gaaaaacttg aagagcttga aattgcttac tttttatata acggtggaaa
     9001 tgacagtatg gatactgttt cgaaaatttc tcgttatgct gaaagggttg gaagcgacat
     9061 caaagttttg ggagtgccta aaacaatcga taatgatctt gttatgacag atcacactcc
     9121 tggttttcca agtgctgcaa gatatgttgc aaacactgtt cgtgaaattg tgacagatgc
     9181 aagcgtttat gaaactccag cagttacaat tatagaaatc atgggacgtc atgctggttg
     9241 gcttacagct gcaagtgtgt tggcgcgtcg cttcgaaggg gatgctcctt acttgatcta
     9301 tcttccagaa gttgattttg atttagatga ttttgtgaag cgtgttgagg ctttgttgaa
     9361 agagcacaac gctgttgttg tttgtgtttc tgaaggaatc aatgatgcgg acggcaagtt
     9421 tatctgtgaa tatgatagtg caaccggcgt agatagcttc ggacataaaa tgcttgctgg
     9481 agctggtaaa ttcttggaga atttgcttcg tgataagtta ggttgtaagg ctcgcagtgt
     9541 tgagtttaac gtttcacagc gttgttcttc ggcgcttatg agcaagaccg atcaagaaga
     9601 agcaattatg gctggccgtt ttggcgttga aagtgcactt gcgggcgaga ccggttacat
     9661 gattgcgttt gatagagtta gtgattataa aattgaatgc agtttgcaag acgttaataa
     9721 aatttgcaac gaagaaaaga aagttccggc ttcaatgatt tg
//
LOCUS       DBNCDC010000036         9486 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_3153_length_9486_cov_6.727388, whole genome shotgun sequence.
ACCESSION   DBNCDC010000036 DBNCDC010000000
VERSION     DBNCDC010000036.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 9486)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9486)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9486
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3153_length_9486_cov_6.727388"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="AC04VQ_03830"
     CDS             <1..660
                     /locus_tag="AC04VQ_03830"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIO9553306.1"
                     /translation="SGVDIGAYDDNGFDLEDLCKSLLEIIGDRIARIRISSIEPNNVS
                     DDLIDIIAGADGKICRHLHMPLQSGSSKVLKEMARHYNADEYLEVVDRLKKKIPNIAL
                     STDIIVGFPGESDADFKDSVEVSKKCGFMKIHVFPYSKRQGTPAADRPDQISDEVKNE
                     RAKVLRELSDELAVKDFKSRAGSIEDVLVENEVQGRSESYHLLDLPEGFTPGDLIKVQ
                     L"
     gene            complement(644..1165)
                     /locus_tag="AC04VQ_03835"
     CDS             complement(644..1165)
                     /locus_tag="AC04VQ_03835"
                     /inference="COORDINATES: protein motif:HMM:NF014707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HIO9553307.1"
                     /translation="MYSNSNIFPKQEFERRFATLEKWASENSIPLIKDDYDETEWSSR
                     VVKYGEAEAKTPERLARCGECYQLRLEHAAKFASANKIKYLSSTLAVSPYQYYEELKK
                     RLAVVCEEYNLTPVLMDFRPFYSEATRESKELNMYRQKYCGCAFSYNESMEQFKRTNK
                     DKYIEELKSIIEP"
     gene            1320..2417
                     /gene="tgt"
                     /locus_tag="AC04VQ_03840"
     CDS             1320..2417
                     /gene="tgt"
                     /locus_tag="AC04VQ_03840"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009608811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIO9553308.1"
                     /translation="MKNFECKIDGKKGKARALTFKTPHGEFQTPMFMPVGTRASVKGI
                     TVPQLHDLGSQVVLANTYHLAMRPGVDIVKKAGGVHKFSNYDGPMLTDSGGFQVFSLA
                     DTVKLEADGVHFKSVYDGADVFWTPEDNMKIQADIGADIIMQLDQCTPYPASREFVND
                     AVELSGAWAKRCLDEHSKNGREDQTLFGIVQGGMELDLRKKSIDLLMKAEEESLANGG
                     RAFGGFGIGGYSVGESHEVMFETLPEVAQSLPEDRPRYLMGVGDEEAIVRAVHEGVDM
                     FDCVLPTRTARMGTAMTSEGSLKMKNAKFSEDFGPLDPKCDCPVCKNYTRAYIRHLFN
                     TNEILGSTLLSYHNLYYLINLCKSLRKEILS"
     gene            2484..3029
                     /locus_tag="AC04VQ_03845"
     CDS             2484..3029
                     /locus_tag="AC04VQ_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIO9553309.1"
                     /translation="MVGKIYRNIIIIQLSISILSLLGSNIDNIIVGKFMSAEALAACG
                     LVLPLIQMTGVLSGVITTGIKTVCSRSIGAGNRQKANDEISTAAVFSIIVFTSLCILG
                     YVFVDPISAALAQSPSGELFDYVKNYMVGYLLVIPSLGILSLFIYILQMNNQAAYCTG
                     AAVAFIICNVGFDLISVFLLD"
     gene            3075..4232
                     /locus_tag="AC04VQ_03850"
     CDS             3075..4232
                     /locus_tag="AC04VQ_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIO9553310.1"
                     /translation="MIGILLTGYFRVNTTLKISFKVFHWKHLGNIVVNGLTNAIATGT
                     SMVIKMIVNMVVLQSAGTDALAAVTVIVSFAGILMAVAKAIAYCTDMASGMFYGERNV
                     KELKNTVYTFVKYSVIFNACIGLVVAATSEWCCLLFINANEVAYPMAVSGLRLFTLSM
                     VFFSISDCFVYFLLGIKKPGYSYVVAAAINISLGVYSALLIPTMMVDGAALAYVCGYA
                     TVFVLIVIFFTIRNRTSPSSPSTYMILPKGFEIPRDHIYEGQPRNKEDVIIMSKFFTE
                     EFFPKFSNDVKKSMAIALAIEELGINVFDHGMKKKRPYLFEIRVIFDEEKGSWIVRTR
                     DDCKEFNPVKYLEMQDSTNPEDQLGLALVLKMAKNAQYYNTMGLNNLVVEF"
     gene            4249..7038
                     /gene="ileS"
                     /locus_tag="AC04VQ_03855"
     CDS             4249..7038
                     /gene="ileS"
                     /locus_tag="AC04VQ_03855"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIO9553311.1"
                     /translation="MSQSFKETMNLPQTEFPMRGNLPENEPKRLDAWDKFDIYHKVLD
                     KNKDGQPFVLHDGPPYANGPIHLGHAFNKILKDFVVKSKAQQGYYTPYIPGWDCHGQP
                     IEHMVEEKLGPEKMAKIDKPTLRRLCREWAEKFVDIQREGFKRLGVNGDWENPYLTFT
                     PNYESGNVEVFKKMYLDGAVYRGRKPIHWCKHCHTALAEAEIEYGDETSPSIFVNFLM
                     DEVPEAFDVDELYILIWTTTPWTLPANTAVSLAPEAEYGVVNANGKNMLFALDMVKDV
                     AEAAGWDKYEVLPGKVLGKELEGYKYTCPIRKDLKGTIIYGDHVTLDSGTGAVHTAPG
                     HGQEDYLVGQQFGIPTLMPVDDNGVLTEEAGQFAGLDVDDSNPKIIEYLKNEGVLVAK
                     QDISHSYPHCWRCHEPVIFRATDQWFVSMDKTGLREKAGKAIVEDVEFIPDWAKNRIG
                     SMVAERPDWCISRQRSWGVPIPVFKCAKCGETVANEATFDAVIKLFEEEGADAWFTKD
                     PSEYLPKGIKCDNCGCEELEPESDILDVWWESGCSHTSVCKHREADGLHFPAEMYLEG
                     SDQHRGWFQSSLLTSIGAYDAPPYKQVMHCGFIVDGEGKKQSKSKGNVIDPADVMKKY
                     GADVLRLWVASVDYSQDVGIDDEILKRVTDSYRTIRNTFKNILGNLYDFDDKTDLVQN
                     WDDLFPADKWIIARIYQVLQDTTEDFNKYRFHSISQRIFNFINNELSPIYMNIAKDRL
                     YSDAKTSHERRSAQTALCNVLEVMVRILSPILSFTCEEVWSYYKADEVESVQLAGWPK
                     LEDFVPHVDEAKLETALADYEKLFEIRDAYTRALEEKVVAGEFKKSQETGAKISLPDS
                     YKDVVESLGANTLEELFVCSDSEIDYGADAVSVEITPAKGEKCPRCWNYRELNAEGVC
                     QRCSEVLSDLDFKFE"
     gene            7103..7558
                     /gene="lspA"
                     /locus_tag="AC04VQ_03860"
     CDS             7103..7558
                     /gene="lspA"
                     /locus_tag="AC04VQ_03860"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIO9553312.1"
                     /translation="MLDRITKIYFDSGQFALGQNISDSILGIFHLTLVHNTGAAFGIF
                     GNSTIVLGIISVIVSIFLLALPFALMNYKGKNNFSYTPTFIQLFAIGIVVSGGIGNAI
                     DRFVTGYVVDFICFDFIDFPVFNIADIGVTCGVIILVTLMMYYSFSKTK"
     gene            complement(7534..8466)
                     /locus_tag="AC04VQ_03865"
     CDS             complement(7534..8466)
                     /locus_tag="AC04VQ_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012798228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIO9553313.1"
                     /translation="MIVVEEYNGMRLDAFLAQVDAYPSRSIAAKFCEANKVLVNGNPK
                     SKNFKLSEGDEVEWEPAVEDVEVEGEDIPLDVRYEDDDIIVISKQPGLVCHPAHGHAN
                     GTLVNALINHCGKKHLCDIQDDHRRLGIVHRLDADTSGLMVCAKNNASGRELTKAMKS
                     HETLRCYKALVYGNIKQDSGKIDVPLLRTLNKRPKMIASNDAKAKTATTTFKVVERIE
                     SEFGNFTLLECKLETGRTHQIRAHMEYIRHPVVGDPLYTGGAPKDFNAEAELGLRRQF
                     LHSYKIGFKHPRSGEYLEFEDKLPKDLYLVLEKL"
     gene            8522..>9486
                     /gene="metG"
                     /locus_tag="AC04VQ_03870"
     CDS             8522..>9486
                     /gene="metG"
                     /locus_tag="AC04VQ_03870"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015761332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIO9553314.1"
                     /translation="MAKETYAFTTPIYYVNAKPHLGTAYTTIASDCVARYQRMNGREV
                     AFVTGMDEHGQKVADTAAANNMSPQEWCDSMEPAFRDEWDNLKITYTDFVRTTEKRQE
                     RSVQKFWHDLYDKGYLYKGAYEGWYCVHEETYYAESDLEKNEDGVYVCPECGRPVQES
                     TGEENWFFKLSEFQDKLLDYYEKHLDFIRPEIRYNEVVSFVKGGLKDLSISRSTFDWG
                     VPLPFDEGHVAYVWADALLAYLTGIGYADPDREEEFNSCWPMQYHFVGKDIIRFHCVI
                     WPAMLMAAGLPQPKCIFGHGFLLTKGEKMSKSKGNAVMPQDIINV"
BASE COUNT         2560 a         1830 c         2238 g         2858 t
ORIGIN      
        1 agcggtgtgg atattggcgc ctatgatgat aatggttttg atttggaaga tttgtgtaaa
       61 agccttcttg aaattatagg ggacagaatc gcgcgaattc gaatttcaag catagagcca
      121 aataatgtta gcgacgatct cattgatatt attgcaggag cggacgggaa aatttgcaga
      181 catttacaca tgccacttca gtcaggatct tcgaaggtgc taaaagaaat ggctcgccat
      241 tataatgcgg atgaatattt ggaagtggtt gaccgcctta aaaagaaaat tccaaacatt
      301 gcgctatcaa ccgatattat tgttggtttt ccaggagaga gcgatgccga cttcaaagat
      361 agcgttgagg tgtccaaaaa atgtggcttt atgaagattc acgtttttcc ttattctaag
      421 aggcagggca ctccagcggc ggatagacct gatcaaatta gtgacgaagt taaaaatgaa
      481 cgcgctaaag ttcttcgtga acttagtgat gaacttgctg ttaaagattt caagagtaga
      541 gcagggagca ttgaagatgt tttggttgaa aatgaagtac aaggccgaag cgaaagttac
      601 cacttgcttg atttgcccga agggtttact cctggagatt tgattaaggt tcaattatag
      661 attttagctc ttcaatgtat ttatctttgt tcgtgcgctt gaactgttcc atactttcat
      721 tataagaaaa agcgcaacca caatatttct gacgatacat atttagctct ttagattctc
      781 gcgtggcttc ggaataaaaa ggacgaaaat ccatcaatac cggcgtcaaa ttgtattctt
      841 cgcaaacaac cgcaagccgc tttttcaatt cttcatagta ttgataaggg ctaacggcca
      901 gcgtagagct caaatattta attttgtttg cacttgcgaa ttttgctgcg tgctcaagac
      961 gaagttgata gcattcgcca cacctggcaa ggcgctcggg agttttggcc tcggcttcgc
     1021 catatttcac tactcgcgaa gaccattcgg tttcatcata atcatcttta attagaggga
     1081 tgctattttc gcttgcccat ttttccaaag tagcgaagcg acgttcgaac tcttgctttg
     1141 ggaaaatgtt ggaatttgag tacatgattg tgatgtctca gcccgcatct ttcaagattt
     1201 tgaaaggctc taaagtgcat gggccgcaac aagcatgaag gacaaggcgt tcgccgggtt
     1261 tcggcgcgtc aatttttgta tctgtttggg ctgtaatcat atattatatg gtatcacata
     1321 tgaaaaattt tgaatgcaaa atagacggaa aaaaaggtaa ggccagggcg ctgactttca
     1381 agactcctca tggcgaattt caaacgccga tgtttatgcc tgttgggact cgtgcgagcg
     1441 tcaaaggcat tacggttccg cagctacacg atttaggcag tcaagttgtg cttgcaaata
     1501 cctatcactt ggcaatgcgt ccaggtgttg atattgttaa aaaagctggt ggtgtccata
     1561 aattttcaaa ttacgatggt cctatgctta ccgattctgg tggctttcaa gtttttagtt
     1621 tggctgacac tgttaagctc gaagctgatg gcgtgcattt taaaagcgtt tatgacggcg
     1681 ccgatgtttt ttggacgcct gaagataata tgaaaattca agcagacatt ggagctgaca
     1741 ttattatgca actggatcag tgcactccat atcctgcaag tcgcgaattt gtaaacgatg
     1801 cggttgagct ttcgggtgct tgggcaaaac gttgcttgga tgagcattca aagaatggtc
     1861 gcgaagacca aacgctattt ggaattgtcc aaggaggcat ggagctggat cttcgtaaga
     1921 agagtattga tctgctcatg aaagccgaag aagagtcgct cgcgaatggc ggtcgcgcct
     1981 tcggaggttt tgggattggc ggttattcag taggcgagag ccacgaagtt atgtttgaga
     2041 cattgccaga agttgcgcaa agtttgccgg aagacaggcc tcgttacctt atgggcgtag
     2101 gggatgaaga ggcgattgtt cgcgcagttc acgagggtgt agacatgttt gattgtgtgc
     2161 ttccaaccag gactgcacgt atgggaacgg ccatgacttc ggagggatct ttgaagatga
     2221 aaaatgcaaa atttagcgaa gacttcggac cgcttgaccc taaatgtgat tgccctgttt
     2281 gcaaaaatta cactcgagcg tatattcgcc atctttttaa tactaatgaa attctcggct
     2341 caacattact aagctatcac aacctatatt atttaattaa cctttgcaaa tcgctaagaa
     2401 aagagattct ttcttagtgt tttctgctaa aataccactt cgcaacataa ggagactata
     2461 taaaaggggg tatagttaag aatatggttg gtaagatata cagaaatatc ataatcattc
     2521 agttgagtat ctcgatttta agtctgcttg gatcaaatat tgacaatatc attgtaggta
     2581 agtttatgag tgccgaagct ctggcagcct gcggtttagt acttccgcta attcaaatga
     2641 caggcgtact ttctggcgtt attacaactg gtattaaaac tgtttgctca agatcgattg
     2701 gtgctggtaa caggcaaaaa gctaacgacg aaatatctac ggcagctgta ttttctatta
     2761 ttgtgtttac atccctttgt atcttaggtt atgtttttgt tgatccaatt tctgctgcac
     2821 ttgctcaaag cccaagtgga gaacttttcg actatgttaa aaactacatg gttggttatt
     2881 tattggtaat tccatcgctt ggaattttgt ctctgttcat ctatatttta caaatgaaca
     2941 atcaggctgc ttattgtacg ggagctgctg tagcgtttat tatttgcaat gttggttttg
     3001 acttaataag tgtttttctt cttgattgag gtctctacgg aattggcctt tcaacttcac
     3061 tttcttattt tgtcatgatt ggaattttgc tcactggtta cttccgtgtc aatacaactc
     3121 ttaaaatttc tttcaaggtt tttcattgga agcatcttgg aaacatcgtc gtcaacggtc
     3181 tgaccaatgc aattgctact ggcacaagca tggttatcaa gatgatagta aacatggttg
     3241 tgcttcaatc cgcaggcacc gatgctctgg ctgccgtaac cgttatcgtt agctttgctg
     3301 gaattttgat ggctgtagct aaggcaattg cttattgtac ggatatggca tcaggcatgt
     3361 tttatggcga gcgcaacgtc aaagaactta aaaacacggt ttataccttc gttaagtatt
     3421 ctgttatttt taatgcttgc ataggcttgg ttgttgcggc tacttcggag tggtgctgtt
     3481 tgttatttat aaatgctaac gaagttgctt atccaatggc agtttctgga cttcgtctat
     3541 ttacgctctc aatggtattt ttctcaattt cagattgctt tgtttacttt ttgcttggca
     3601 ttaaaaagcc aggttactca tatgttgttg cagctgctat taatattagt ttgggtgttt
     3661 attcggcctt gctaattcca acaatgatgg tcgatggtgc agcgcttgct tatgtgtgcg
     3721 gttacgcgac tgtatttgtt ttgattgtca ttttctttac aatccgaaac aggacaagtc
     3781 cttcgagccc ttcaacttat atgattttgc caaagggttt tgaaattcct cgagaccata
     3841 tttacgaagg tcagcctcgc aataaggaag acgttattat tatgtcgaag tttttcaccg
     3901 aagaattctt cccgaagttt agcaatgatg tcaaaaagag catggcaatt gcgctggcta
     3961 tcgaggagct tggcataaat gtttttgatc atggcatgaa aaagaaaaga ccgtatttgt
     4021 ttgaaattcg tgtaattttt gacgaagaaa aaggctcctg gattgttcgt accagggatg
     4081 actgcaaaga attcaaccct gttaaatatc ttgagatgca agacagcact aaccccgaag
     4141 accaacttgg tttagccctt gtattaaaaa tggctaaaaa tgcgcaatat tataatacaa
     4201 tggggcttaa caacttggta gtcgagtttt aagaaggggt tacatatcat gtcgcagtcg
     4261 tttaaagaaa caatgaactt gccacagact gaatttccga tgcgagggaa tttgcctgaa
     4321 aacgagccta aacgcttaga tgcttgggat aagtttgata tttaccataa agttttagac
     4381 aagaataagg atggccagcc atttgttttg catgatggtc cgccttatgc aaatggtcca
     4441 attcacttgg gtcatgcctt caataaaatt ttgaaggact tcgttgtaaa gagcaaggct
     4501 cagcaaggat attacacccc ttatattcca ggttgggact gtcacggtca gccaattgag
     4561 catatggtcg aagaaaagct tggacctgag aaaatggcga agatcgacaa gccgacgctt
     4621 cgtcgtttgt gcagggaatg ggccgagaaa tttgttgata ttcaacgtga gggctttaag
     4681 cgccttggcg ttaatggtga ttgggaaaat ccatatttga ctttcactcc aaattacgaa
     4741 tctggaaatg ttgaagtttt caaaaagatg tatttggatg gggcggtcta tcgtggtcgc
     4801 aagccaattc attggtgtaa gcattgccac acggccttgg ccgaggcaga aattgaatat
     4861 ggcgatgaga cctctccttc gatatttgta aactttttga tggatgaagt tccggaagca
     4921 tttgatgttg acgagctcta cattttaatt tggacaacaa ctccttggac gcttccagct
     4981 aacactgctg tgtctttggc tcccgaagct gaatacggag ttgtaaacgc taacggtaaa
     5041 aacatgcttt ttgctcttga tatggtcaag gacgttgccg aagctgcagg ctgggataaa
     5101 tatgaagttt tgcctggcaa ggtgctcggc aaagagctgg aaggatataa atacacttgt
     5161 ccaattcgca aggatttaaa aggcacaata atttatggtg atcacgtaac gcttgatagt
     5221 ggtactggtg ctgttcatac cgctcctgga catggtcaag aggattatct ggtcgggcag
     5281 cagtttggta ttccaacgct tatgccggtt gatgataatg gtgtgcttac cgaagaggcc
     5341 gggcagtttg cgggtttgga tgttgatgat tcgaatccaa agattattga atatttaaag
     5401 aacgaaggcg tgcttgttgc taagcaagac attagccaca gttatcctca ttgttggcgt
     5461 tgtcatgagc ctgtaatttt ccgtgcaaca gaccagtggt ttgtaagcat ggacaagact
     5521 ggacttcgcg aaaaagctgg aaaagcaatt gttgaagatg ttgagtttat tccagattgg
     5581 gcaaagaaca gaattggtag catggttgcc gaacgtcctg attggtgtat ttctcgccag
     5641 cgttcatggg gtgttccaat ccctgttttc aagtgcgcga agtgcggcga gaccgttgca
     5701 aatgaagcca ccttcgatgc tgttattaag ctcttcgaag aagagggcgc ggatgcttgg
     5761 tttactaaag atccaagtga atatttgcct aagggtatta aatgtgataa ttgcggttgc
     5821 gaagaactcg aacccgaaag tgacatttta gatgtttggt gggagagtgg ttgttcgcac
     5881 acaagtgttt gcaagcaccg cgaagctgac ggtcttcact tcccagcaga gatgtatttg
     5941 gaaggatcag accagcacag aggatggttc caatcttcgc ttctaacttc aattggcgct
     6001 tatgatgcgc ctccatataa gcaagttatg cattgcggat ttattgtgga tggcgaaggt
     6061 aagaagcaat ctaagtctaa gggcaacgtt attgacccag ctgacgtcat gaaaaagtat
     6121 ggcgcggatg tgcttcgtct ttgggttgca agcgttgatt acagccagga tgttggaatt
     6181 gatgatgaaa ttttgaagcg cgttactgat tcgtatcgaa caattcgaaa cactttcaaa
     6241 aacattttgg gcaaccttta tgattttgac gacaagactg atctagtcca aaactgggat
     6301 gatctctttc cggcggataa gtggataatt gctcgtattt accaagtttt gcaagatacc
     6361 accgaggact ttaacaagta ccgcttccat agtatttcgc agcgaatttt caacttcatc
     6421 aataatgaac tttcgccaat ttatatgaat attgccaagg accgacttta ttccgatgca
     6481 aaaacttcgc acgaacgccg aagtgctcaa actgcgcttt gcaatgtgct tgaggttatg
     6541 gttcgcattt taagtccaat ccttagcttt acttgcgagg aagtttggtc ttactataaa
     6601 gctgacgaag tcgagagcgt acagcttgcg ggctggccaa agcttgagga ctttgttccg
     6661 cacgtggacg aagcaaagct cgaaactgct ttagctgatt acgaaaaact ttttgaaatt
     6721 cgtgatgcct atactcgtgc gcttgaagaa aaagttgttg cgggtgagtt taaaaagagt
     6781 caagagaccg gcgcgaagat ttcgcttccg gattcttaca aagatgttgt tgaatctctt
     6841 ggcgctaaca cacttgaaga gctcttcgtt tgttcagaca gcgaaatcga ttatggtgcc
     6901 gatgctgttt cggttgaaat cactccggcg aagggcgaga agtgccctcg ttgctggaat
     6961 tatcgcgaat taaacgccga aggcgtttgc cagcgttgct ctgaggtttt aagcgatctt
     7021 gattttaaat ttgaatagaa ctcaaacaaa tagttttaag aaaactgcag taatactttt
     7081 tgttgttgct gcagtttgat ttattcttga cagaatcaca aaaatttatt tcgatagcgg
     7141 acaattcgct ttgggtcaaa atatcagtga ttcaatattg ggaatctttc atctcacgtt
     7201 agttcacaat accggagcgg cattcgggat cttcggtaat tcgaccatcg tgcttggaat
     7261 aatttcggtt attgtttcaa tctttttact ggcgcttcct tttgcgctta tgaattacaa
     7321 aggcaagaat aattttagct acacgccaac cttcattcaa cttttcgcaa taggcattgt
     7381 tgtttcgggt ggcataggca acgcgatcga tcgcttcgtc acgggctacg tggttgactt
     7441 tatctgcttt gattttatcg actttccagt ctttaacatc gcagacatcg gcgtcacctg
     7501 cggcgtcatc atcctcgtca cattaatgat gtattatagt ttttctaaaa ctaaataaag
     7561 gtctttgggg agtttgtctt cgaattcgag gtattcgccc gagcgaggat gcttaaatcc
     7621 aattttatat gaatgcagaa attgacggcg aagtcccagt tcagcttcgg cgttaaaatc
     7681 ttttggagct ccacctgtgt agagcgggtc gccaaccacg gggtgacgaa tatattccat
     7741 atgtgcacga atttggtgcg ttcgaccagt ttcaagctta cattctaaaa gagtaaagtt
     7801 tccaaattcg gattcaatac gttcaacgac cttaaaggtt gtggttgccg tcttcgcctt
     7861 cgcatcgtta gaggcaatca tttttggcct cttattcagg gtccgaagca gcggcacatc
     7921 aatcttgcca ctatcttgct taatgtttcc ataaacgagc gctttataac aacgaagcgt
     7981 ctcgtgggat ttcatcgctt ttgtaagctc tcgaccagaa gcattgtttt tcgcgcaaac
     8041 catcaatccc gaagtgtcag catccaagcg gtgaacaatt ccaagtcgcc tgtggtcatc
     8101 ctgaatatca caaaggtgct ttttaccgca atgatttatt agcgcattta caagtgttcc
     8161 gtttgcgtga ccatgtgcag gatggcaaac caaaccaggc tgtttagaaa tcacgatgat
     8221 gtcatcgtct tcatagcgaa cgtcaagcgg gatgtcttcg ccctccactt cgacatcttc
     8281 aacggccggc tcccactcga cttcgtcacc ttcactcaat ttgaaatttt tcgacttagg
     8341 gttgccgttg acaaggactt tattagcctc gcaaaatttc gcagcaatac ttcgtgaagg
     8401 gtaagcgtca acctgcgcta aaaacgcatc caaacgcata ccattatatt cttcaacaac
     8461 tatcatcccg cgtaattata gcaaaaagtg gtaaaatacg aggttgcgaa aaggagagat
     8521 tatggctaaa gagacatatg cgtttaccac gccaatatat tatgtaaatg cgaagccgca
     8581 tttgggaacg gcttacacaa cgattgcttc ggactgcgtt gcgcgttatc agcgaatgaa
     8641 tggtcgcgaa gttgcgtttg taactggcat ggatgagcac ggccaaaagg ttgctgatac
     8701 tgcggctgcc aacaatatgt ctccacagga atggtgcgat agcatggagc ctgcgtttcg
     8761 cgatgagtgg gataatctca aaatcacata tactgacttc gttcgcacga ccgaaaagcg
     8821 tcaggagcga agcgttcaaa agttttggca tgacctctac gacaagggtt acctctacaa
     8881 aggcgcttat gagggctggt attgcgttca cgaggaaacc tactacgccg aaagcgacct
     8941 tgagaaaaac gaagatgggg tctacgtttg tcctgagtgc ggccgtcctg ttcaggaaag
     9001 cacgggtgag gagaattggt ttttcaagct aagcgaattc caggacaagc tccttgatta
     9061 ttatgaaaag catctggatt ttattcgtcc ggaaattcga tataacgaag ttgtgtcttt
     9121 tgtgaagggc ggacttaagg atttatccat ttcacgaagt acttttgact ggggtgttcc
     9181 gcttccattt gacgaaggcc acgttgccta cgtttgggct gatgctctgc ttgcatacct
     9241 tacaggaatt ggatatgcag atccggatcg cgaggaagag ttcaacagtt gttggccgat
     9301 gcagtatcac ttcgttggta aagatattat tcgcttccat tgcgttattt ggccagcgat
     9361 gcttatggcg gctggacttc cgcagcctaa gtgcattttt ggtcatggat ttttgctaac
     9421 caagggcgag aaaatgtcga agagcaaggg caatgctgtt atgcctcaag acataattaa
     9481 tgtttt
//
LOCUS       DBNCDC010000037         9357 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_3244_length_9357_cov_8.196947, whole genome shotgun sequence.
ACCESSION   DBNCDC010000037 DBNCDC010000000
VERSION     DBNCDC010000037.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9357)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3244_length_9357_cov_8.196947"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            17..2551
                     /locus_tag="AC04VQ_03875"
     CDS             17..2551
                     /locus_tag="AC04VQ_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIO9553315.1"
                     /translation="MASFDKFTDKAKKALTLARDEAHALKQAYVGTEHVLLGLIDEND
                     GFAAKTLAKLNVTHDDVLETIREIIPIDPNADLSKHASFTPRMKQVMENAFREAMQSG
                     QSFIATEHLLLGIVRDEESNGVKVLKKLNVTTDQVREALNDVIGGATVYAGKAPLGND
                     NPDSILEAFGVDLNKRAKANKLDPVIGRAAEIERVMQILTRRQKNNPLLIGEPGVGKT
                     AIVEGLAELIVSKRVPDVLQGMRIVMLDISALVAGSKFRGEFEERLKKCIKEVREAGD
                     IILFIDEMHTIIGAGAAEGSIDAASILKPPLSRGEIQVIGATTSEEYRKHLEKDTALE
                     RRFQTVTIEEPTEEQTIRIVDGLRDKYEGHHNVKYSDEALKASVTLSARYIQDRFLPD
                     KAIDLLDEAGARMRIRNMQLPQELRDLDAKIRKVRATKDNYTAKQEFDKAGKMHEEEI
                     ELLKQREAEEKKLEEKSKSKVHEVSVEDIANVVSMVTGVPVHNLTEAEAEKLLRMEDV
                     LHKRVIGQNEAVTALSKAIRRSRSGLKDPNRPSGSFIFLGPSGVGKTELSKALAEFLF
                     NSEDALISFDMSEYMEQHSVSKLVGSPPGYVGYDEGGQLTKAVRKKPYSVVLFDEIEK
                     AHPDIFNILLQILDEGRLTDGQGHNVDFRNTIIIMTSNVGARQISQTKTLGFDSGDGK
                     GMSDEEIKSAATSELKKLFRPEFLNRVDETIVFKSLGEKEIIQIVDLMVDDLRERLIA
                     QNMSINLTKAACKLIAKEGTNVTYGARPLRRAIQRLIEDPVSEEILAGKYKDGSVIKV
                     DAKDDKLTFTAAKGKIPQPKKKDTIARDTELILKDFGLSNTGVQKD"
     gene            2561..3439
                     /gene="ispD"
                     /locus_tag="AC04VQ_03880"
     CDS             2561..3439
                     /gene="ispD"
                     /locus_tag="AC04VQ_03880"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015778862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HIO9553316.1"
                     /translation="MTDEQKHTEEIDAFEDDSPVNLFDRIRLSQPENFSMKKIPGEVS
                     PKADCAAIILAGGLGRRLNDGSKNHKKISKQLLKMSGKPVLTWSMDSFDAVPEIGAII
                     VVCPEEQFQEYIETVVDPFAFQTPIVMAPAGDTRQESAFHGLDMVPEKFDYVMIHDGA
                     RPLVPPTVIKHIYSVLKGNPDIDGAVCAHPCIDTLKVVEDGKIVGTPDRSAFWLAQTP
                     QIFKRETYTEAHRAALSDGFVGSDDASLIERIGGNVKVVEGKRNNIKLTVPEDYLILA
                     SVLQALNLEHGSEYDL"
     gene            3488..3961
                     /gene="ispF"
                     /locus_tag="AC04VQ_03885"
     CDS             3488..3961
                     /gene="ispF"
                     /locus_tag="AC04VQ_03885"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015778861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIO9553317.1"
                     /translation="MKIGIGYDVHKLVSGRKLILGGVEIAYEKGLDGHSDADVLCHAI
                     GDAILGAAKLGDIGQHFPDTDPKYKDANSLKLLNEITKIISDKGFTVINVDSVVIAQA
                     PKLAPLRDKMRENIASVLGIAVENVGVKATTTEGLGFEGRGEGISAQAVALLSEE"
     gene            3961..5223
                     /gene="cysS"
                     /locus_tag="AC04VQ_03890"
                     /pseudo
     CDS             3961..5223
                     /gene="cysS"
                     /locus_tag="AC04VQ_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013565.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
     gene            5212..5445
                     /locus_tag="AC04VQ_03895"
     CDS             5212..5445
                     /locus_tag="AC04VQ_03895"
                     /inference="COORDINATES: protein motif:HMM:NF049034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysS/YqeB C-terminal domain-containing protein"
                     /protein_id="HIO9553318.1"
                     /translation="MRVFGIEFSADEKDSGEFEALAEKLGIEIKGDAEKSILDERSEA
                     RANKNFELADKIRDTLAEAGYTIEDTPQGSRIV"
     gene            complement(5442..6185)
                     /locus_tag="AC04VQ_03900"
                     /pseudo
     CDS             complement(5442..6185)
                     /locus_tag="AC04VQ_03900"
                     /inference="COORDINATES: protein motif:HMM:NF046761.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Kae1-like domain-containing protein"
     gene            complement(6198..6407)
                     /locus_tag="AC04VQ_03905"
                     /pseudo
     CDS             complement(6198..6407)
                     /locus_tag="AC04VQ_03905"
                     /inference="COORDINATES: protein motif:HMM:NF036810.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6423..7412)
                     /locus_tag="AC04VQ_03910"
     CDS             complement(6423..7412)
                     /locus_tag="AC04VQ_03910"
                     /inference="COORDINATES: protein motif:HMM:NF013468.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HIO9553319.1"
                     /translation="MCTFKMCGTCESEYLDPADRRFHAQPNACFNCGPAVSWITAGKL
                     KRSKCTDSQGAGVGTLGPLFIGDTREKSDEIFSNCVKFLRDGKIVAIKGLGGFHLACD
                     ATNNEAVARLRSKKMRSNKAFAIMASDLQSIKKICHVNAAEEKLLSGSARPIVLLKSK
                     RTNTLAENVCMGLPEIGVMLPYTPVQHILMHDFSLAGGKFLVMTSGNLYDNPIVTDDA
                     EAYEVLGDVADAFLGNNREILARYDDSVVRVIDAAGSDAVQMIRRARGYAPAPIKIKL
                     NKPAEVFATGAEQKNTFAFSRPIPETEPSAKVNTEVFVSQHIGDIENADIFKT"
     gene            complement(7458..7886)
                     /locus_tag="AC04VQ_03915"
                     /pseudo
     CDS             complement(7458..7886)
                     /locus_tag="AC04VQ_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012911.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
     gene            complement(8050..8556)
                     /gene="tmk"
                     /locus_tag="AC04VQ_03920"
     CDS             complement(8050..8556)
                     /gene="tmk"
                     /locus_tag="AC04VQ_03920"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIO9553320.1"
                     /translation="MADGIFITLEGGDGAGKTTHLMKLADKLREMGKEVVIVREPGGT
                     RIGEKIRNILLDNENDEMTARTELFLYEAARSQIVAEVIKPALHEGKVVLCDRFFDST
                     VCYQGYGRGLDVDEIKKANLFTTDGLKPDRTVIIFTDTVENSIYRATKNDGADRIEAE
                     GEAFQKKV"
     gene            complement(8620..>9357)
                     /locus_tag="AC04VQ_03925"
     CDS             complement(8620..>9357)
                     /locus_tag="AC04VQ_03925"
                     /inference="COORDINATES: protein motif:HMM:NF017494.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HIO9553321.1"
                     /translation="DIPCRELTEDGGCSGELTAEIIEEALNKGLAAEPGTAERAAASV
                     KEVMDKAAAKREADGKSMNAGEGQDEAIKLQLPLSRDDVKNLKAGDKVLLSGPIYTLR
                     DAGHIRLLDEMEEGKPLPYGLDGQTIYYAGPSPAAAGRPFGAIGPTTATRMDFAAPTL
                     YENGIVATIGKGHRNEAVHESCKKTGSVFFCTIGGCAAYLAKCIKSAELIAYEDLGTE
                     ALRKCEVEDFPVFVGIDTNGIDVYDHD"
BASE COUNT         2755 a         2007 c         2046 g         2549 t
ORIGIN      
        1 ttttgaaagg tgagaaatgg catcttttga caaatttaca gataaggcga aaaaagcgct
       61 aactttggcg cgcgacgaag cacatgccct caagcaggca tatgttggca ctgagcacgt
      121 gcttttaggc ttgattgacg aaaacgatgg cttcgctgca aagaccctcg ctaaattaaa
      181 tgttactcac gatgacgttt tggaaacaat tcgtgaaatt atacctattg atcctaacgc
      241 tgaccttagc aaacacgcaa gtttcacgcc tcgcatgaag caagttatgg aaaatgcttt
      301 ccgcgaagca atgcaaagtg gccaaagctt tattgcaact gagcacctgc ttttaggaat
      361 tgttcgcgac gaagaaagca acggcgttaa ggttttaaag aaacttaatg ttacaaccga
      421 tcaggtccgc gaggctctaa atgacgttat tggtggcgcc actgtttatg ctggtaaagc
      481 tccacttgga aatgacaatc ccgacagcat tcttgaggct tttggcgttg acctaaataa
      541 gcgcgcaaaa gccaacaagc ttgaccctgt tattggccga gctgctgaaa ttgagcgcgt
      601 tatgcaaatt ttgactcgcc gccaaaagaa caatcctctg cttattggtg agcctggtgt
      661 tggtaaaact gcaattgtcg aaggcctggc agagctcatc gttagcaagc gtgtccctga
      721 tgttttgcaa ggcatgcgaa ttgttatgct tgacatttct gcccttgttg caggttcgaa
      781 attccgtggt gaattcgaag agcgtcttaa aaaatgcatt aaggaagtcc gcgaagctgg
      841 agacatcatc ctcttcattg atgaaatgca tacaataatt ggcgctggtg cagccgaagg
      901 ttccatcgac gctgcaagta tcctaaagcc tccactttct cgtggggaaa ttcaagtcat
      961 tggtgccacg acttcggagg aatatagaaa acacttggag aaggacacgg cgttggagcg
     1021 caggttccaa actgtcacta ttgaagagcc aaccgaagag caaacaatcc gcattgtgga
     1081 tgggcttcgt gataaatatg agggccatca caatgttaaa tattctgacg aggcgctaaa
     1141 agcttctgta actttgtcgg caagatatat tcaagaccgc tttttacctg ataaggccat
     1201 tgatttgctc gacgaagccg gagctcgcat gcgcattcga aatatgcaac ttccgcaaga
     1261 acttcgtgat ttggatgcaa aaattcgcaa agttcgtgcg acaaaagaca actacacggc
     1321 caaacaagaa tttgacaaag ccggaaaaat gcacgaggaa gaaattgaac ttcttaagca
     1381 acgcgaagcg gaagagaaaa agctcgaaga aaaatcgaag tctaaggttc acgaagttag
     1441 tgttgaagac attgcaaatg ttgtttcaat ggtgactggt gttcctgttc ataatttgac
     1501 cgaagccgag gccgaaaagt tattgcgcat ggaagatgtt ttgcacaagc gtgttatcgg
     1561 acaaaacgaa gctgtaactg cgctttcaaa ggcgattcgt aggtctcgaa gcggacttaa
     1621 agatccaaac agaccttcgg gttcgtttat tttcctcgga ccttctggcg ttggtaaaac
     1681 cgaactttca aaagcgctcg ctgaattttt gttcaatagc gaagacgcgc ttatctcgtt
     1741 tgacatgagt gaatacatgg aacagcattc tgtttcgaag ttggttggaa gccctccggg
     1801 ctatgttgga tatgacgaag gtggccagct taccaaagct gttcgcaaaa aaccttacag
     1861 cgttgtgctc ttcgacgaaa ttgagaaggc acatcctgac atcttcaaca ttttgcttca
     1921 aattcttgac gaaggcagac tcaccgatgg ccaaggtcat aatgttgatt ttagaaatac
     1981 aattatcatt atgacttcaa atgttggtgc tcgtcaaatt tcgcaaacca aaactttggg
     2041 ttttgacagc ggagatggca aaggcatgag cgacgaagaa atcaagagcg ctgcaacttc
     2101 ggagctgaaa aaacttttcc gccctgaatt tttgaaccgc gttgatgaaa ccatcgtttt
     2161 caagagtctt ggcgaaaaag aaattattca aattgttgat ttgatggttg atgaccttcg
     2221 cgagcgtttg atcgcgcaaa atatgtcgat caatttgaca aaggctgctt gcaaacttat
     2281 tgcgaaggaa gggacaaacg tcacatatgg tgcgcgtccg cttcgtcgtg caattcagcg
     2341 actcatcgaa gacccagttt cggaagaaat tttagctgga aaatataaag atgggtctgt
     2401 tattaaagtt gatgcgaagg atgacaagct cactttcacc gctgctaagg gtaagattcc
     2461 gcagcctaag aaaaaggata caatcgctcg agacactgaa ctaattttaa aggacttcgg
     2521 gctaagtaat acaggcgttc aaaaagatta aggggcaaat atgactgacg aacaaaaaca
     2581 cactgaggaa attgatgcct ttgaagatga ttccccagtc aatttgtttg acaggatacg
     2641 cctaagtcaa cctgaaaatt tttcaatgaa aaaaattcct ggcgaagtgt ctccgaaggc
     2701 cgattgcgct gcaataattt tggctggcgg tcttggaaga agacttaacg atggaagcaa
     2761 aaaccacaaa aagatttcaa agcaacttct aaagatgtct ggcaagccgg ttctaacctg
     2821 gtcgatggac tcttttgatg ctgttcctga aattggagca attattgttg tttgtccgga
     2881 agaacaattt caagaatata ttgaaactgt tgttgaccca tttgcttttc aaacgccaat
     2941 tgttatggct cccgctggag acactcgtca agaaagtgct ttccatggac ttgatatggt
     3001 tcctgaaaaa tttgactacg ttatgattca cgatggcgcg cgtccgcttg ttccgccaac
     3061 cgtcatcaag catatatata gtgtgcttaa gggcaatcca gatatcgatg gcgcggtttg
     3121 tgcacatcca tgcattgaca cactaaaagt tgtcgaagat ggaaaaattg ttggaacgcc
     3181 tgatagaagt gcgttttggc ttgctcaaac accgcaaatt tttaagcgcg aaacctatac
     3241 cgaagctcat agggctgcac tctccgatgg atttgtggga agcgatgatg cttcgttgat
     3301 tgagagaatt ggtggaaacg tgaaagttgt cgaaggtaag cgcaacaaca ttaagctaac
     3361 ggtgcctgaa gattatttga ttttggcaag tgttttgcaa gcgcttaacc ttgaacacgg
     3421 ctcggaatac gacctatagg tgtaattctt gcctggcaat aattacacct tttaaggaaa
     3481 gaggaaaatg aaaattggaa ttggatatga tgttcataaa ttggtttctg gtcggaagct
     3541 gattttaggt ggcgttgaaa ttgcttatga aaaagggctt gatggtcatt ccgatgccga
     3601 tgtcttgtgc catgcaattg gtgatgcaat tttaggcgcg gcaaaacttg gagacattgg
     3661 tcagcacttc ccggacacag atccaaaata taaagatgca aattcgttga agcttttaaa
     3721 tgaaattaca aaaattatta gcgacaaagg cttcactgtc ataaacgttg atagcgtagt
     3781 tattgctcaa gcgccgaagc ttgctccgct tcgtgacaaa atgcgcgaaa acattgcaag
     3841 cgttttgggg attgcagttg aaaatgttgg agtaaaagct acaacgaccg aaggccttgg
     3901 cttcgaaggg cggggtgaag gaatttcggc acaggcagtt gcactacttt cggaggaata
     3961 atgcttaaga tttatagcac acagcataga aaaaaagttg actttaagcc agttcacgaa
     4021 ggcaaaattg gaatgtatgt ttgcgggcca acggtttata acagaattca cattggcaac
     4081 gcgcgcactt ttcttagctt cgatattatt cgtcgctatc ttgaatgaag cggttatgat
     4141 gtgaccttcg ttcaaaatgt aactgacgtt gatgacaaaa ttattaatcg cgctgccgaa
     4201 gaaaacacga ccgcggccga gattgctaaa aaatacaccg accttttcat cgaagacatg
     4261 cacgccgccg gtgttaaaga tcctgacatt cgtcctaagg ctaccgaaga aattgacgcc
     4321 atgattgaac ttgttcaaag tttggttgat aagggtcatg cttacgttga agacggcgac
     4381 gtctactttg acgttcgtag ttttaaggga tatgggtccc tgagcgggcg tgatgtcgaa
     4441 gatgccgaaa gcggtcaccg cgaacttcaa gcggatggtc aaggtttgga agaacgcaaa
     4501 cacgccgaag ctgacttcgc gctttgaaag gcagccaagc ctggcgagcc ttcgtgagat
     4561 agtccgtggg gtccggggcg tccgggttgg cacattgaat gctcagcaat gtctcacaaa
     4621 tatttaggat tgccattcga tattcacggc ggcggatcag atttggtctt ccctcatcat
     4681 gaaaatgagc gcgcacaaag tatgtgtggt tacgaaaatg attttgcaaa ttattggatg
     4741 catgccggaa tgcttcaagt tgcaaatgat gaatctggca aaaatgaaaa gatgagcaag
     4801 agcttaaata acttcgtttt gctatatgag gctcttgaac aggtcaaacc taacgttctt
     4861 cgcatgcttg ttttgcaaac tcattaccgc agtcctcttg tttttggcga gaagagactt
     4921 accgaagccg agtccgcttt gactcgaatt gaaactgcga ttaaaaatat tgattggaag
     4981 cttcaaaacc aaagtgataa atctgaaatt gatacaaaag caattgacga tgcttgcaca
     5041 aaagcaaaag aaaaattcac cgaggcgatg gatgatgact tcaacacaag tttagcgctt
     5101 gctgaaattt ttgaacttgt tggtgttatc aatacagaaa ttggtaagtc agaaatcaac
     5161 aaagatactg ctccagctat tgaaaaagct aaggcaatga tcacagaatt gatgagagtt
     5221 tttggaattg aattcagtgc cgatgaaaaa gactctggcg aatttgaagc cttggctgaa
     5281 aaacttggca tcgaaattaa aggcgacgct gaaaaatcta ttcttgatga acgcagtgag
     5341 gctcgtgcaa acaaaaattt cgaacttgcc gacaagattc gagacacttt agccgaagcg
     5401 ggctacacca tcgaagacac accgcaaggt tcacgtatag tttagatttg accgagagaa
     5461 attccaccat cgttgggtgg aacgtctacg ttaagagcaa cagaaaaacc ctgttcaaca
     5521 agcattttga ttgttttttc aattaggtac ctgttcataa aaacgccacc cgataaagca
     5581 agatcattaa taccataaac ttgttttgat aattgagcgc aagttaagat tgcatttgca
     5641 aaagcatcgt gaaagttctt cgcaattttt ccaacttcaa ttccatcttt taaatcatcc
     5701 aacattgctt tgaaagtgtt tgcgcaatca aataaaacaa cggatgtatc ttgtgcggtt
     5761 gatttttctg ttccaacatt ttttgtaatt tgaatttcat aacatttgtc aaacttcgaa
     5821 ttttttgccc gtgagttttg acttaaataa tctcaaagtt tggcttcaag catgattgct
     5881 gcttcgcctt cgtaggttgg agctgggcaa atgcctaaaa tcgccgaagc cgcatcgaaa
     5941 agtcttccaa cagatgaagt catcggcgtg ttaatgccat cttcaatcat cgtgttcaaa
     6001 ttttctaaat cagacttttt ataatcctta aaaacttttt tcgtttgcgg atgatccaac
     6061 aaatcaaaag ttcaaagcgc gccataagcc aaacgaagcg gatccttaat tgctgccgcg
     6121 cctccgggca ttggaatgta agtgaaattt gcgaagcgct caaagtcttg tgtgttgcac
     6181 agcaaggctt cgccgcctca aatattttta tcaacgccat aacccgttcc gtcaaatgca
     6241 aaacctaaaa ccggaccagt caatccattt tcatacatta cgctgcgaat atgcgcataa
     6301 tgatgctgaa ccttatccgc ttccttcaca tattttgaag ttaaatattc agggtgtaaa
     6361 tccgaaacaa ttttttttgg tttgaattta aagatttttt caaattgatt ttttgtttct
     6421 tctcaagttt taaaaatgtc agcgttttca atatctccaa tgtgttgcga cacaaaaact
     6481 tccgtattaa cttttgcgct cggctccgtt tctggaattg gtcgtgaaaa tgcaaaagta
     6541 tttttttgct cggcaccggt tgcaaaaact tcggcgggct tattcaattt aattttgata
     6601 ggagcaggcg cataacctcg cgctcgacga atcatctgaa cagcatcact acctgcggcg
     6661 tcaatgactc gtacaacact gtcatcatag cgcgccaaaa tttcacgatt gtttccaaga
     6721 aatgcgtcag ccacatcacc caaaacttcg taggcctctg catcgtcagt cacgattgga
     6781 ttgtcatata aatttccaga agtcatgacc aaaaattttc cgccggccaa cgaaaaatca
     6841 tgcatcaaaa tatgttggac cggagtatat ggaagcatca ctccaatctc cggcaaaccc
     6901 atgcaaacat tttccgctaa agtatttgtg cgtttcgact tcagcaaaac aattggccga
     6961 gcagatccac tcaagagctt ttcttcggca gcatttacat gacaaatctt cttaatagat
     7021 tgcaggtcag atgccataat tgcaaaagcc ttattgcttc gcattttttt cgaacgaaga
     7081 cgcgcaaccg cttcgttgtt agtcgcgtcg catgcaagat gaaaaccacc aagccctttg
     7141 atggctacaa tttttccatc gcgcaaaaac ttaacgcagt ttgaaaatat ttcatcagat
     7201 ttttctcgag tgtccccaat aaaaagcggc ccaagagtac cgacccccgc cccctgggaa
     7261 tcggtacact tggaccgctt aagtttaccg gccgtaatcc agctgactgc agggccgcaa
     7321 ttaaaacacg cattaggttg cgcatgaaat cgtcggtcag ctggatcaag gtactcgctt
     7381 tcgcaagtcc cacacatttt aaaagtgcac attgaagtat agcatctatc ataaggtaat
     7441 tgaccaataa ttgtaaatct tggcccgcaa tttgtacaat ttataaaagg atacctgtac
     7501 ctgcggtctt tagggtcaaa aagttcacga acgcaatctt cgcaagttgc taaatccgga
     7561 gaaattaaag tttgtcccgc cgaagccccc gccttcgagg tttttttgtc ttccgaagtt
     7621 ttaatctcaa aagtagtaaa gccttcaagc ggaacctcgc gcatttcaat ttcttcaaca
     7681 cgagcagcag ccggcgcctc gtttgaaatt gcgaggcaaa attcatcgac gagtttttct
     7741 tcgccttcgg cgtgaatgtg aacaccatcc gcagaattat aaactcaacc agaaatcaaa
     7801 tgccgcttgg caagtctata aataaaagga cggaaaccga ctccttgaac gattcccgtc
     7861 acatgtattt ttagcgctgt ctgcataatt tctaaaaggt gtaattattg ccaggcatga
     7921 aatacacctt ttttaaaaga ggtcttttac tgcgtcaaaa acgagcttga aagtttcatc
     7981 ttttgttggc tgcaaattaa cgaggcgaat tcgaccgggt tcctgtttag ccagctcaag
     8041 aaagccattt caaactttct tttggaaggc ctcgccttca gcttcgatac ggtcagcccc
     8101 atcatttttt gttgcgcgat aaattgaatt ttcaaccgtg tctgtaaaaa tgattaccgt
     8161 acggtctggt ttgagaccat cggtcgtaaa aagattcgcc tttttgattt catcaacgtc
     8221 caaaccgcgt ccataacctt gataacaaac agtgctgtcg aaaaagcgat cgcacaaaac
     8281 aaccttgcct tcgtgaagcg caggtttaat aacctcggca acaatttgcg aacgtgcagc
     8341 ttcgtataag aaaagttcgg tgcgggcagt catttcatcg ttttcgttat ccaacaaaat
     8401 attacgaatc ttttcgccaa tcctggtacc accaggctca cgaacaataa caacttcctt
     8461 acccatttcg cgaagtttgt cagcaagctt cataagatgc gttgtcttgc cggcgccatc
     8521 gccgccttcc aaagttataa aaattccatc agccataaat tctcctaaaa tcgagtaaat
     8581 gccacgagta aatcgagtac tgaccccggg gaaatcgagt tagtcatgat cgtagacatc
     8641 gattccgttt gtgtctattc ctacaaagac ggggaaatcc tcaacttcgc atttgcgaag
     8701 agcttcggtg cccaaatctt cataagcgat aagttcagca ctcttaatgc attttgcaag
     8761 ataagccgcg caaccaccaa tggtgcagaa gaaaacgctg ccagtctttt tgcaagattc
     8821 atgaacagct tcgtttctgt gacccttgcc aatagttgca acaattccgt tttcatataa
     8881 tgttggagct gcaaaatcca tacgagttgc agttgttggt ccaatagctc cgaagggacg
     8941 tcccgccgca gccgggcttg gacctgcata ataaatagtt tgaccatcta acccgtaagg
     9001 aagtggtttt ccttcttcca tttcatcgag caaacgaatg tgtccagcat cacgaagtgt
     9061 ataaattgga ccagagagca aaactttgtc cccagctttt aaattcttga catcatcacg
     9121 gctaagtgga agttgcaatt tgattgcttc gtcctgacct tcgccagcat tcatgctctt
     9181 accatcagcc tcgcgctttg cagcagcctt atccataact tctttcaccg aagccgcagc
     9241 acgctcagcc gttccaggct cagcagcaag acctttgttc aaagcctctt caataatttc
     9301 agcagtaagc tcgccagagc aaccgccatc ttcagtaagt tcacgacaag gaatgtc
//
LOCUS       DBNCDC010000038         9185 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_3371_length_9185_cov_8.574589, whole genome shotgun sequence.
ACCESSION   DBNCDC010000038 DBNCDC010000000
VERSION     DBNCDC010000038.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9185)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9185
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3371_length_9185_cov_8.574589"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            185..826
                     /gene="infC"
                     /locus_tag="AC04VQ_03930"
     CDS             185..826
                     /gene="infC"
                     /locus_tag="AC04VQ_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIO9553322.1"
                     /translation="MAKDDLRVNRAIRAQECVLVGYDGQQLGNYAVSDALEMAQREGL
                     DLVEVTPKQAVPVCRIMDYGKYKYERSRKEKANRKKAGKIETKEMKFRPKIDVGDYDT
                     KKKHVLRFLGQGNRVKITIMFRGREMAHPEQGLSILEKLSSELEDIAVVEAAPKMEGR
                     NMFMTIAPLPEFVKKSQAEIEEKQTQGKNSRRNREAAARAAEKAKESEGGQDA"
     gene            819..1016
                     /gene="rpmI"
                     /locus_tag="AC04VQ_03935"
     CDS             819..1016
                     /gene="rpmI"
                     /locus_tag="AC04VQ_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013979589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIO9553323.1"
                     /translation="MPKMKTHKGTAKRFRVTGSGKIMRGKAYKSHILAKKSQKRKRNF
                     RKETEVSTADNKTIARNLGRR"
     gene            1044..1397
                     /gene="rplT"
                     /locus_tag="AC04VQ_03940"
     CDS             1044..1397
                     /gene="rplT"
                     /locus_tag="AC04VQ_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIO9553324.1"
                     /translation="MARVKRSTHARKKRREVLGEAKGYYGQKSRSYRNAKQQVMKSMQ
                     YQYRDRRNKKRVIRSLWIVRINAAARENGMSYSTFINGLKKAGVELDRKVLADMAVNE
                     KKAFAKLAEIAKKAS"
     gene            1470..2573
                     /gene="alr"
                     /locus_tag="AC04VQ_03945"
     CDS             1470..2573
                     /gene="alr"
                     /locus_tag="AC04VQ_03945"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIO9553325.1"
                     /translation="MDAIRHNINLTKSKIGPNCKLLVIVKADAYGHGAVQIAKTAINC
                     GAAYLGVATVDEAMELRDEKIKSNILVLSQPPVESIPLLVKHDIMPAIHTTEFAIQYA
                     EAADQAGKSAPYHLAVNTGMNRVGVRHNDVLDFLKSVSFHRALKLEGTFTHFATADVA
                     DEMTFKKQMSLFTQLIDNMKINDYDPGIVHAANSAAIFRYPESHFNMVRLGVCMYGYY
                     PSDLIKQYVELEPSMSVHARIVDERILGIGEGVSYGHKYRARSSAKICTIPIGYADGL
                     HREATGKIGVIYKGKIFPQVGSICMDMCMFEIDMNSAIAQTNFNAQIGDEVILVGCDG
                     EAEITVDDLAKASGTINYEIICSFSRRMPVLYV"
     gene            2582..3193
                     /locus_tag="AC04VQ_03950"
     CDS             2582..3193
                     /locus_tag="AC04VQ_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIO9553326.1"
                     /translation="MNKDDYLNKEIILDSVFRESEFERTEKILLVGKNGKKEGPFIRK
                     IFKENKGLGEIYKVLLNERNVGLNCDGIPNVYYAGREGDKLIIVEECFIGISLTDFIK
                     KHNFDINLFNTIYLQICEAVDYLHTGFSKPIIHRGINPENILVNEDSHKIQLIDFGIS
                     QHYNEDEDQPTLKSGSRRAFKADVRNDIYSLGKVLEFMMDANN"
     gene            3413..3634
                     /locus_tag="AC04VQ_03955"
     CDS             3413..3634
                     /locus_tag="AC04VQ_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553327.1"
                     /translation="MASAFVIYLAATPEIDLIGPLTVAEKIIYSAMTILFIMYPIYFV
                     LLFKPSLRKMVKSLPRLNAIHYIAFVISC"
     gene            complement(3673..4746)
                     /gene="hypD"
                     /locus_tag="AC04VQ_03960"
                     /pseudo
     CDS             complement(3673..4746)
                     /gene="hypD"
                     /locus_tag="AC04VQ_03960"
                     /inference="COORDINATES: protein motif:HMM:NF014034.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
     gene            complement(4837..4998)
                     /locus_tag="AC04VQ_03965"
     CDS             complement(4837..4998)
                     /locus_tag="AC04VQ_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553328.1"
                     /translation="MKAKVYDGKTYVDFNFVKELQKLENANNYTKVGSNDISPSRSYE
                     NIGPLKMPA"
     gene            complement(5140..5316)
                     /locus_tag="AC04VQ_03970"
     CDS             complement(5140..5316)
                     /locus_tag="AC04VQ_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553329.1"
                     /translation="MNTENSYSVKTVDGIHKLKEIAPFSPYVDVVLAAKMLGWNIDED
                     KSQQHNSNEYDKAN"
     gene            complement(5374..5538)
                     /locus_tag="AC04VQ_03975"
     CDS             complement(5374..5538)
                     /locus_tag="AC04VQ_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553330.1"
                     /translation="MNAIAAQKRDKTKSQLLDYWHFYEHYIVPMANEVLHEAANSSDA
                     QGAVNDFCTT"
     gene            complement(5668..6489)
                     /locus_tag="AC04VQ_03980"
                     /pseudo
     CDS             complement(5668..6489)
                     /locus_tag="AC04VQ_03980"
                     /inference="COORDINATES: protein motif:HMM:NF015536.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
     gene            complement(6505..6771)
                     /locus_tag="AC04VQ_03985"
     CDS             complement(6505..6771)
                     /locus_tag="AC04VQ_03985"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HIO9553331.1"
                     /translation="MKNLFQTHPILSKLFICLIASLCAILVCTTPAFACTGIYVGSDA
                     CAEGTTIIARSDDTSPYDKAIFAKVYGGQDGEQIKHLKAKNGFE"
     gene            complement(6771..6983)
                     /locus_tag="AC04VQ_03990"
     CDS             complement(6771..6983)
                     /locus_tag="AC04VQ_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006362376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HIO9553332.1"
                     /translation="MCLAIPGKIKSINEDKVAEVDFDGVMRTASMDMLPKAKIGDYVL
                     VHAGFAIEIVDKEEAKKTLDLLKELS"
     gene            7061..8428
                     /locus_tag="AC04VQ_03995"
                     /pseudo
     CDS             7061..8428
                     /locus_tag="AC04VQ_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139449.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            8482..8703
                     /locus_tag="AC04VQ_04000"
     CDS             8482..8703
                     /locus_tag="AC04VQ_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553333.1"
                     /translation="MSKLGKKTILIAYASTGSGHKIAAEAIYEEFLNKDEDINIVVAD
                     ILDYFPQNESGSKFVNFTTGLLSPVFDLT"
     gene            8785..>9185
                     /locus_tag="AC04VQ_04005"
     CDS             8785..>9185
                     /locus_tag="AC04VQ_04005"
                     /inference="COORDINATES: protein motif:HMM:NF018613.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009247 - glycolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGDG synthase family glycosyltransferase"
                     /protein_id="HIO9553334.1"
                     /translation="MAGLNPDVVVCTHFVCANVAAKVRAIKNLNFRIVSVPTDYETEG
                     LWPHKETDLFCVGSEEMASTLQSRRVDRSKILVSGIPISSEFSNKYSKLEVKKLFSIK
                     QNKKVVLIICGANDSGPYKNMRKTLNACLSF"
BASE COUNT         2777 a         1583 c         1797 g         3028 t
ORIGIN      
        1 taatatacgt ggtccgacag aagcgaaggc aaatagcctt ccacctactg gttgaaaaat
       61 actgatgggt aatacaaata ggaagccgaa attcggcaac ttgagcagca aaagctgtag
      121 atgtattacc cgccaatgag cgggtttttt gttgggaaag tgttgtttaa cagaaaggtt
      181 gtaagtggca aaagacgatc tacgtgttaa ccgtgcaatt cgtgcacaag aatgtgtgct
      241 tgttggttat gatggccagc aacttggaaa ttatgcggta tccgatgctt tggaaatggc
      301 tcaacgcgaa ggtcttgacc tcgttgaagt aactccgaag caagctgtgc ctgtatgcag
      361 aattatggat tatggaaagt ataagtatga gcgcagccga aaagaaaagg ccaaccgtaa
      421 aaaagctggc aaaattgaaa ccaaggaaat gaagtttcgt ccaaaaattg atgttggtga
      481 ttatgacacc aagaaaaagc atgtgcttcg tttcttaggt caaggaaaca gagtaaaaat
      541 tactatcatg tttcgaggtc gcgagatggc tcatccagaa caaggtttga gcattttgga
      601 aaaactttca agtgagctcg aagacatcgc cgttgttgaa gcggccccaa aaatggaagg
      661 cagaaacatg tttatgacaa ttgctccgct tccagaattt gtaaaaaaat ctcaagcaga
      721 gattgaagaa aaacaaacac aaggaaaaaa cagcagacgt aatcgcgaag ctgcggctcg
      781 tgctgctgag aaagcaaaag agtctgaagg aggacaagat gcctaagatg aaaacacaca
      841 aaggaactgc aaagcgcttc cgcgttactg gttctggaaa aattatgcgc ggtaaggcat
      901 acaaaagtca cattttggca aagaaaagcc agaagcgcaa aagaaacttt cgtaaggaaa
      961 ctgaagtttc tactgcagac aacaaaacaa ttgcaaggaa tttaggccgc cgataaggca
     1021 gcgtttataa aggagtgatt taaatggcta gagtaaaaag atctacacat gcacgcaaaa
     1081 agcgtcgcga agtcctgggt gaagctaagg gttattatgg ccaaaaatcg cgttcgtata
     1141 gaaacgctaa acaacaggtc atgaaatcca tgcaatatca gtatcgtgat agacgcaata
     1201 aaaagcgtgt tattcgcagc ttatggattg ttcgtattaa tgcagctgct cgcgaaaacg
     1261 gaatgagcta ttcaactttt atcaacggtt tgaaaaaggc cggagttgaa cttgaccgta
     1321 aagttttagc agatatggct gttaatgaga agaaagcgtt tgccaaactt gctgaaattg
     1381 caaaaaaggc aagctaagtg aaatttcgca atacaaaatt agaaggaatt gagactaaaa
     1441 ataggcgttg gtcttgatta caagttgact tggatgctat tcgtcataat attaatttga
     1501 cgaagtcaaa gattggtcca aattgtaaat tgcttgtaat tgtcaaggcc gatgcttatg
     1561 gtcatggtgc tgtccaaatt gcaaagacag caataaattg tggtgctgct tatcttggag
     1621 ttgcaactgt tgacgaagca atggagcttc gtgatgaaaa gattaaaagc aatattttag
     1681 ttttgtccca gcctccagtt gaatcaattc cacttttggt taagcatgac attatgccag
     1741 caattcacac cactgaattt gctattcaat atgccgaagc ggcagatcaa gcgggtaaga
     1801 gcgctcccta tcatttagct gtaaacacag gaatgaatag ggttggcgtt cgacataatg
     1861 atgtacttga cttcttaaaa agtgttagct ttcatcgcgc acttaagttg gaaggcacat
     1921 ttacacattt tgcaactgca gatgttgctg atgaaatgac tttcaaaaag caaatgtctt
     1981 tgttcacaca attgattgat aatatgaaga tcaacgatta cgatccggga attgttcacg
     2041 ccgcaaactc ggcagcaatt ttccgctatc cagaaagtca ttttaatatg gttcgtttgg
     2101 gtgtttgcat gtatggttat tacccaagcg atttaatcaa acaatatgta gaacttgaac
     2161 cttcaatgag tgttcatgca agaattgttg atgagcgaat cttgggaatt ggtgaaggcg
     2221 tgtcttacgg tcacaagtac agggccaggt cttcggcaaa aatttgcaca attccaattg
     2281 gatatgccga tggacttcat cgcgaagcaa caggaaaaat tggtgtaatt tataagggaa
     2341 aaattttccc acaggttggt tcaatttgca tggatatgtg tatgtttgaa attgatatga
     2401 attctgcaat tgctcaaaca aatttcaacg ctcaaattgg tgacgaagtt atacttgttg
     2461 gatgtgatgg cgaagctgaa attactgttg atgatttagc aaaagcatca ggtacaatta
     2521 actacgaaat tatttgttct tttagtcgac gtatgcctgt tttatatgta taggttaaac
     2581 gatgaataaa gacgattatt taaataaaga aataattctt gattcggttt ttagagaaag
     2641 tgaattcgag aggactgaaa agattcttct tgttggaaaa aatggcaaga aagaaggtcc
     2701 ttttattcgt aagattttta aagaaaacaa aggccttgga gaaatttata aagtacttct
     2761 taacgaaaga aatgttggcc ttaattgtga tggaattcct aacgtttatt atgcaggcag
     2821 agaaggcgac aaactaatta ttgttgaaga atgcttcatt ggaatttctc tgactgactt
     2881 cataaaaaag cataattttg acatcaattt atttaataca atttatttgc aaatttgcga
     2941 agctgttgat tatttgcaca ctggttttag taagccaatt attcatcgcg gtattaatcc
     3001 tgaaaatatt ttggtgaacg aagactcaca caaaattcaa cttatagact ttggtatttc
     3061 tcagcattac aacgaagacg aagaccaacc aaccttaaag tctggttcca ggcgtgcgtt
     3121 taaagcagat gttagaaatg acatttattc gcttggaaaa gttcttgagt tcatgatgga
     3181 tgccaacaat tgagcaggtg gcaatgccga ttttcctaat gatatttata aggaaattgt
     3241 tcaaaaggct acagattctg aacctgcaaa cagatacaac agtgtagcta caatgattat
     3301 tgaatttcaa agtgcaacaa agcgtagaaa gtggattcaa gacatttatg aggataagcg
     3361 aattattgtg gtcggtcata tttgaaatgc tttagtcaca gttgcttaca ttttggcttc
     3421 agcctttgtt atttatttgg cagcgactcc tgaaattgat ttaataggtc ctttgactgt
     3481 agctgaaaaa ataatttata gcgctatgac tattttgttt attatgtatc caatttattt
     3541 cgttctttta tttaagccat ctcttcgaaa gatggttaaa tctcttccgc gattaaatgc
     3601 cattcattat attgcatttg taatttcttg ttgatcagtt gtgcttgtcc tctgcttgat
     3661 cgcacaactt ttctaatctt gatatttgaa atgcgcggag cacgaacctt cgttcgacac
     3721 catacaaggt ccaattggat tttctgggtt gcaagttttt ccgaataacg ggcagtcagt
     3781 cggttggata attcctttta ggacgcttcc acatttgcat gctttgtttt cttttgtagg
     3841 ttcaggtttg atattaaatt tgtgttctgc atcaaaagcg gaatacttcc ttcggatttt
     3901 atatcctgac gaagcaatag acccaaggcc ccttcaattt gaatcgcaag tttcaaaaac
     3961 ttcgttgatt aaatcaagag cggtttggtt accttcgcca cgcacacctc gcgggtaagc
     4021 attttcaatt tttgcctcac catttttaat ttgagtcact aacatatcaa ttgcttgcat
     4081 tatgtcagct gcctcaaatc cagaaacaac tcctggcaac ttataattct ttgctaaaaa
     4141 ttcataaatc gaaagcccag ttatagttga tacatgacca ggtaaaataa gacctgaaac
     4201 ttgaactttg tcatcgtttg caattaaatc taatgctccc ggcatatttt tatgagcgca
     4261 aagaatgctg aagtttgcaa gtgccatttc ttcggcacga attattgttg ctgcggtagt
     4321 tggtgttgtt gtttcaaaac caattcccaa aaacacaact tcttttttag atttttttgc
     4381 aatttcaatt gcatcaagcg gcgaataaac aatttctata tcagcgccgt ttgctttttc
     4441 ttttataaga gacgaagttg agcccggaac ccgcatcatg tctccaaatg tagcaatagt
     4501 taagtttgga atttttgtaa gcgctatcat tttatcgatt gtttcgtttg gagtaacgca
     4561 aacagggcaa ccaggaccag atattagctt tatatttttt ggaagaagct tgcgaatgcc
     4621 gaagcgcgca atcgacattg tgtgcgtgcc gcaaacttcc atcaaattta tttgtggtag
     4681 accttcagct tttttgctaa tgctagactg tagtttctcg actaagtttt gtgctttatc
     4741 agccaagctt tatttacctt ttttccttga ccatacaata atataaacta atgcaatgat
     4801 aattatcgca acgccaacaa tagctaaaac ctggactcaa gcaggcattt ttaatggacc
     4861 aatattctcg taggaacgag aaggagaaat atcgttagaa ccaacctttg tataattatt
     4921 tgcgttttca agtttttgta attcttttac gaaattaaaa tcaacataag tttttccatc
     4981 ataaactttc gctttcatat catagctttg cccattaatt gttatttgac ttttcgaaag
     5041 tctatgtcca ttgcctgtgt gaatttcagc ggagtttttt ccattagaaa tttttacata
     5101 gccttctgtg cctccattgt cggcttgctc ttcgcttcct caatttgctt tatcatattc
     5161 attagaatta tgttgctgac ttttatcttc gtcaatattc cagccaagca tttttgctgc
     5221 aagcacaaca tcaacatatg gtgaaaatgg agcaatttct ttaagtttat gaattccatc
     5281 tacagtttta acgctataag aattttcagt attcatgtag tatcattcaa cttcgtcact
     5341 tagacgtttt gcatcaaaat aattttgagc ttgtcatgtt gtacaaaaat catttactgc
     5401 gccttgagca tccgaagaat tggcagcctc gtgtagaact tcgtttgcca ttggaacaat
     5461 atagtgctca taaaaatgcc aatagtctaa tagttgagat ttagttttgt ctcttttttg
     5521 agcagcaatt gcattcaatt ttttaaacgc cgaaaatgca ttctcgtctt caagattaaa
     5581 gtagttttta ttttcatacg tataagccgg atcaaaagaa ctttctaaat tcgaatttgg
     5641 gattcaagta ttaaattcag aacttgatca tgttacccag tttagtaaac acatatttgc
     5701 aggtaaatcc ttaaaaattt gtgtcagaag aatgctttgc gaagtttcgg tgttgataga
     5761 tctataagtg ttgttgccat ttacatctaa actaaattct gttccttcaa acctatcgcg
     5821 ataaatatcc attatgtcat taacgctaac ttttttgtct ggcttataaa ataacggaat
     5881 ttttgtatga acattataag gagtagaagt gcttgggctt aaaatgtttt gaactcttca
     5941 agttctttga cgacacacat ccatttctac ttcagttccc gcatatgttt tgtaaatatt
     6001 catttttcca tcgctatcat aaacagcgaa gcccttttcc gccggaagtt taaacaaatc
     6061 tttagaacaa attacatcag gatttgtctc gtcaatagtt tcaattaaaa agtcattgcc
     6121 tgttccagtt acgcaatcgc ttggagcttt tagcgcacaa tattgatgtc caccataaat
     6181 ttccatatac caaacttcat tttgatcggc aatcattata atgttgtctt cagcagaacc
     6241 tttttcatcg atgatttttg cagtaagttc aacaccttct cttgctgttg cagcttgagc
     6301 acaaattaca agaggaaata catcttcgag tatgccatct ttaacataag ggttatgttc
     6361 ttgcgctttt ttatttgttt cgccagttac tgttgaatta atgtagactc caagatcatt
     6421 cattccaact gaataatagc aagcgccatc ggcaacagca acagtaggat agcaaaccgt
     6481 tctatgaatt ttttccggga agtctcattc aaaaccgttc tttgctttta agtgtttaat
     6541 ttgttctcca tcttgacctc cataaacttt tgcaaaaata gctttgtcat atgggcttgt
     6601 gtcgtctgat cttgcaataa ttgttgtgcc ttctgcgcat gcgtcgcttc caacataaat
     6661 tccagtacat gcaaacgcag gcgttgtgca aactaatatt gcacacagtg atgctatcaa
     6721 gcaaataaat agttttgaaa gaattggatg agtttgaaat aaatttttca ttagcttaac
     6781 tcctttaata gatctaaggt tttttttgct tcttctttat ctacaatttc aattgcgaat
     6841 ccagcatgaa ctaatacata atcgccaatt tttgctttag gaagcatgtc catcgaagca
     6901 gtgcgcatca ctccatcgaa gtcaacttcg gcgactttat cttcgtttat gctttttatt
     6961 tttcctggaa tcgctaaaca catattgatt aatactcttt ctacaaaata tattctacca
     7021 tattttatgt tataataaat cccctgtcga aaggttaatc atggaagcat ttatagctat
     7081 agttacacag attgatactt ggatttgggg tattccaatg attgcaatct taacaatttc
     7141 acacatattt acaacttgaa gaactggttt tattcaactc aaaattccga gggctattaa
     7201 actttcggtt accaatgatc caaatgcaga aggagatatt agccagtttg gcgctttgtg
     7261 cactgcactt tcgggtacat taggtgttgg atgtattgtt ggtgttgcca ctgctattat
     7321 ttatggtggc ccaggtgctg ttttttgaat gtgaatcatg ggtattttag gcattgcaac
     7381 taagtattcc gaaacattta ttgcaattaa atatcgtgtt aaagatagaa aaggaaaaat
     7441 gtgcggcgga gctatgtatg tttgagaacg cgcatttaaa aagaagaata aagacggaag
     7501 cgaaggcaag actccttggt gggctaaaat tggagcaatt tgtttcgctg tttttacagc
     7561 cattgcaact cttggtacag gttctgcaat tcaagcatct gcaatttcag aatcaatcgc
     7621 attttatttt ccagattttc ctcagtggat tattgcaata ataatttgtg tagtaacttt
     7681 ttttgttatt tatggaggcg ttaaaacaat ttcaaatgta tgtgaaaagc ttgttccttt
     7741 catgactgtt gcttatttac ttggaactct tgcgctttta attatgaatt gggaatattt
     7801 tattcctgct gttgttgcaa ttgtacaaag tgcttttagt gatcaagcaa tttttggagg
     7861 cgctattggt tccggcattt ctgttgcatt gcaatatgga atggctcgtg ggcttttctc
     7921 aaacgaatct ggtttgggaa ctgctccaat tgttgcttct gctgctgcta ctaaaaaccc
     7981 agcgcgtcaa tctttggttg caatgaccgg tgctttttgg tctactgttg taatttgtgc
     8041 aattacggga atcgcacttg ttggaagcat gatggcaaat ccagaattgg ttggtgccga
     8101 agctcagaca ctcacgcttc aagctttcca acaaattcct tttattggcg gaccaatgtt
     8161 gtgcttctct cttgcgatgt ttgcattttc aacaattatt ggatggtctt attatgctaa
     8221 tcgttcaatt tgttatctat tcggacatag ggctgttcaa gtttttatgg ttttatattt
     8281 gattgctgca tttattggag gagctggctt tggagatgta atttgaggaa tttcagatat
     8341 taccaatgct ttaatggcta ttcctaatgt agttgttgtt gtattgcttt caaaagttat
     8401 tggtaaagaa actcacgatt acgtttgaga aaacaatctt gatgcccctt ctaacgatgt
     8461 ggttgttgag gttgatcatt aatgtccaaa cttggcaaaa aaacgattct tatagcctat
     8521 gcttctactg gttctggtca taaaattgca gctgaagcaa tatatgaaga gtttttaaac
     8581 aaagatgagg acattaatat agttgttgcc gatattttag attactttcc tcaaaatgaa
     8641 agtggttcaa agtttgttaa ctttactaca ggtttgcttt ctccagtttt tgatttaacc
     8701 tgaagaacaa atttcactgg acgaatttta tgaggcggtg gtcaactttg gccaagtctt
     8761 ttatataaaa atttcgaaca ttatattgct ggtcttaatc cggatgttgt tgtttgcaca
     8821 cattttgttt gtgcaaatgt agcagcaaaa gttcgagcca tcaaaaattt gaattttaga
     8881 attgtatctg tgccaaccga ttatgaaact gaaggtttat ggcctcataa agaaaccgat
     8941 ttgttttgtg taggaagcga agagatggct tcgacgcttc aaagtagaag agtagaccgt
     9001 agcaaaattt tagtttcagg cattccaata tcttcggaat tttcaaataa atattcaaag
     9061 cttgaagtta aaaaattatt ttctattaag caaaacaaga aagttgtgtt gataatttgt
     9121 ggtgctaatg attctggacc ttataaaaat atgagaaaga ctttaaatgc atgtttaagt
     9181 ttttt
//
LOCUS       DBNCDC010000039         9052 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_3463_length_9052_cov_7.293876, whole genome shotgun sequence.
ACCESSION   DBNCDC010000039 DBNCDC010000000
VERSION     DBNCDC010000039.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 9052)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9052)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9052
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3463_length_9052_cov_7.293876"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="AC04VQ_04010"
     CDS             <1..732
                     /locus_tag="AC04VQ_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIO9553335.1"
                     /translation="VDLEVDALRRARITRNHTATHILHWALREVLGDHVNQAGSHVAP
                     DRLRFDFTHFEALNADQIAEVEKLANDQIMRAFDVETYNTSLKEARAKGVTALFGEKY
                     GEEVRVVNVGNFSEELCGGCHVKNTGEIGFVKIINESSVGANARRIEACTSYDAYNYT
                     SGLIAEMDKAASLLKGNKTQLTQNVSKMLANVKELKKELEFSHSKQSTMIRKEVFDDL
                     AQSSMGFDCLVLDCGDIRNYDFKEY"
     gene            739..987
                     /locus_tag="AC04VQ_04015"
     CDS             739..987
                     /locus_tag="AC04VQ_04015"
                     /inference="COORDINATES: protein motif:HMM:NF014342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HIO9553336.1"
                     /translation="MVDHCGDNCAAVLAGTKGEKVLLLAAGTSKAVKAGFNAVDIIKK
                     ISPCINGGGGGQDRMAQAGGTKAEGVAEALKIAKDLLK"
     gene            991..1440
                     /gene="ruvX"
                     /locus_tag="AC04VQ_04020"
     CDS             991..1440
                     /gene="ruvX"
                     /locus_tag="AC04VQ_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012797941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIO9553337.1"
                     /translation="MNVLALDIGDARVGIASGNDSSQIATPLKVLSLDDIMNKSKDFQ
                     LILQDYLPEKFIIGLPKSMDGEENVQAAHVKDIADEISLLYGLPVEFVDERLSSVEAK
                     AILREQGLSEKEMRGKVDSVAASLFLETWLDQQNRVNATSKSSKRTE"
     gene            1463..4228
                     /gene="polA"
                     /locus_tag="AC04VQ_04025"
     CDS             1463..4228
                     /gene="polA"
                     /locus_tag="AC04VQ_04025"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIO9553338.1"
                     /translation="MKKIAVIDGNSWTHRAYHAVPPVMKLADGTPTNAVHGFMNMFLS
                     FYEKAQPDAVICAFDAGIPQFRLEAVEQYKAQRKPMDNELRVQFPIIEEMLESMGIPV
                     VKVEGWEGDDVLGTIADKCEKLGFETLLVTGDKDANQLATDLTKIVNMKKGVSDVAIL
                     GPAEVKEKYGVSVDQFIDYLALMGDSSDNIPGVPGIGPKSASALLQKYESLEGLYEHI
                     DELKGKQQDNIRDNKDAAYVSRTAATISKDVPIDIDLENIAFPNFDQDTVSETFLKFR
                     MRSALSGVLKLIGVVPEESSPEDSKAEASQPRPAIKKEEKVVLKEPKLVFENYVEGED
                     ALALSKKLIKGDFAIAISHKQEAPVQASLFDEEIASKYPEYKNLLLGISNNKETAILE
                     GDSAIETLVEITKYNHEVIAENAKAIFEILFDEFIGADDFYKLNFYDVSLAAYLLNSS
                     KSEYSAVDAWQQVFGSLLPTLDIEPEEGEKKTEKSFIDKKNKILQLRLARRAAASFAL
                     REFLDKRLSEDNADKIYKDIEIPLTKVLVQMQVTGTKIDRAMLDKLSVKTAKLLEHLK
                     SEILELAGEEFNIDSPKQLSHILFEVLGLPAKKKTKTGYSTDAEVLADLSAIHPLPAK
                     IIEYREYAKLKSTYIDALPALADKNDIVHTTYNQKVTTTGRLSSSDPNIQNIPTRSEF
                     GNQIRECFLPLQKDEVFLTADYSQIELRVLAHLSQDKGLIEAFTSGNDFHAHTAANVF
                     GVSVDKVTPDMRRKAKAVNFGIVYGQQAFGLSQSLKISMGEAKQMIDSYFSSYPQVRK
                     YLDRVVEDATLKGYAETMFGRRRYIPELTATNKRFRGFGERTAMNHPMQGSAADIIKI
                     AMIRVSDSIRKQSLKSKLMLQVHDELDLSVDKSEIDTVTELVKTEMENAVKLEVPLVV
                     DIATGEN"
     gene            4225..5337
                     /locus_tag="AC04VQ_04030"
     CDS             4225..5337
                     /locus_tag="AC04VQ_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIO9553339.1"
                     /translation="MSEGRITGLSSVEVEERVKAGKVNTDAGLKTRSIKRICYDNIFT
                     LFNAVNVILAIIVALTGSFKNMLFMGVIVCNTAIGIIQEIRSKKTTDKLTIVAKAKVK
                     ALRDGKTVEINSDEIVEDDIIELGRGDQVPADCIVVEGFCDCNESLLTGESDLIAKQK
                     DSKLMSGSFINSGKVYAKVTHVGAASYANKITAEAKVHKMVNSQIMETMNGLIRIISI
                     IIFPLGALLFAKAYFFNGIDLIQSILTTVSAMIGMIPEGLILLTSTVLAVAVVRLAKH
                     KVLVQQLYCIETLARVDVFCADKTGTITSGKMLLDKIVPIKKDSEDELGRAIKNINDA
                     DDDKNETALAIENYFKGRKAEKLSLKKVIPFSSDKK"
     gene            5434..6537
                     /locus_tag="AC04VQ_04035"
     CDS             5434..6537
                     /locus_tag="AC04VQ_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIO9553340.1"
                     /translation="MAVDSRVLLVAKVDGFDDKGNIKGKPKPLGFICIKDEIRDTAAD
                     TIKFFQDEGVTVKVISGDDPRTVSGIAKTVGVANAEKYVDATTLKTDEEIAEAVQNFA
                     VFGRVKPEQKKAFVIALQKFGHTVAMTGDGVNDVLSLKQADCSIAMASGSAAARNVAH
                     LVLLENDFATMTNVVAEGRRSINNLQRSASLFLVKTLLSMSLALIFLFMPWGYPFQPI
                     QMTLISAFTIGVPSFVLALEPNHDRIKGHFLTNVIVRSIPSAAVIVIAILFINIIGYN
                     FFNFDYGQISTMCVLTMSIIGFMLVIRLSIPFTPIRIGLIIVIVGGTILGCVFFGNLF
                     NIAEYTTEMALFHAITCAVGCGLFFVFYKIVDI"
     gene            6682..7500
                     /locus_tag="AC04VQ_04040"
     CDS             6682..7500
                     /locus_tag="AC04VQ_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HIO9553341.1"
                     /translation="MKVAIACVVVLTLALSSLFISRNHKTANADPSAVSNYIEAKAYL
                     SSVRGEYNAIMSEATSIYNQIEATTKKAFEAQRAMTESQLQLNSLLKYQYENSTIFSI
                     FIMVACSTSLDDFSKNIDYANSAMDYLFNVSKDNKEKKDAFDSTLQNLNSQNAAQNAC
                     LASASEKLAEAETVLQGIRAKLTPEELAELEGEISDIGGGGGGGGGGDTPTPGPTPPG
                     PQPGPSPDPPTPGWSSGLASAYGGSSDPYTPNPGTTATGEVCDDWSTGVAVPMA"
     gene            7782..8042
                     /locus_tag="AC04VQ_04045"
     CDS             7782..8042
                     /locus_tag="AC04VQ_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553342.1"
                     /translation="MIVIGILLYCVYYFKYRATSLACQYALKGYITADEMDEIRRSGD
                     IVLGEEIEDREGNIVVLQLNDKGEYVAYVVRDANGDEPHTHV"
     gene            8064..8198
                     /locus_tag="AC04VQ_04050"
     CDS             8064..8198
                     /locus_tag="AC04VQ_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553343.1"
                     /translation="MVEVGSKFVADGVNNGEEYGHYEPIYEEQPYTDYYYCSECGVHK
                     "
     gene            8268..8837
                     /locus_tag="AC04VQ_04055"
     CDS             8268..8837
                     /locus_tag="AC04VQ_04055"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIO9553344.1"
                     /translation="MAKFVDGESHTFDEYLLIPRHTSEKCTPDRVSLKTPLVKFKKGT
                     KPSLSMNIPMVSAIMQAVSGDRLAVALAKEGGVSFIYGSQPIEKQAEMVYRAKRVKAG
                     FVDSDSNLKPDDTLADVLALKEKTGHSTVAITEDGTSAGKLVGIVTGRDYRISRMKPD
                     TKVKSFMTPKEKLIYAKDGATLSECNDII"
     gene            8892..>9052
                     /locus_tag="AC04VQ_04060"
     CDS             8892..>9052
                     /locus_tag="AC04VQ_04060"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIO9553345.1"
                     /translation="MVFRKDYESHKEHPDELLDNTKRYIVGAGINSRDYKDRIPALVE
                     AGADVLCIDS"
BASE COUNT         2627 a         1628 c         2170 g         2627 t
ORIGIN      
        1 gttgatcttg aggttgatgc gcttcgtcgt gcaagaatta ctagaaacca cacggcaact
       61 catattttgc attgggcgct tcgcgaggtt ttgggcgatc atgttaatca ggctggctca
      121 catgttgcac cggatcgtct tcgttttgac tttacgcatt ttgaagcact aaacgctgat
      181 caaattgccg aggttgagaa gctcgcgaat gatcaaatta tgcgtgcctt tgatgttgaa
      241 acttataata caagcctaaa ggaagcaagg gcgaagggag ttactgctct tttcggtgag
      301 aaatacggcg aagaagttcg cgttgttaac gttggaaatt tcagcgaaga gctttgcggt
      361 ggttgtcacg ttaaaaacac aggcgagatt ggcttcgtta agattattaa tgaaagttct
      421 gttggtgcga atgctcgcag aattgaggct tgcaccagtt acgatgctta taattatact
      481 tctggtttaa ttgcggaaat ggataaggct gcttcgcttt tgaagggtaa caagactcag
      541 cttacacaaa atgtttcaaa gatgcttgcc aatgttaaag agctgaaaaa ggagcttgag
      601 ttttctcaca gcaagcaatc gactatgatt cgcaaggaag ttttcgatga tttggcacag
      661 tcgagtatgg gttttgactg tcttgttctt gattgcggag atattcgaaa ttatgacttt
      721 aaagaatatt gagatgcaat tgttgatcac tgcggtgata attgtgctgc tgttttggca
      781 ggaacgaagg gtgaaaaagt gttgctgctt gcggccggaa cttcgaaggc tgttaaggca
      841 ggatttaacg ctgttgatat tatcaagaaa atttcgcctt gcattaatgg tggcggcggt
      901 ggccaagata gaatggctca agctggaggc acaaaagccg aaggtgtcgc cgaagccttg
      961 aaaatcgcga aagatttgct caaataaatc atgaatgttt tagcgcttga cataggagat
     1021 gctcgagttg gtatcgcctc gggtaacgat agtagtcaaa ttgctacgcc cttaaaagtt
     1081 ctaagtttgg acgacataat gaataagtcc aaagattttc aacttatttt gcaagactac
     1141 ttgcctgaga agtttattat tggattgccg aagtcgatgg acggcgaaga gaacgttcag
     1201 gctgctcatg tgaaagatat tgcggatgag atttcgcttc tttatggctt gccggttgag
     1261 tttgttgatg agcgcctaag ttctgtcgag gcgaaggcga ttcttcgcga gcaaggtttg
     1321 agtgagaaag aaatgcgagg caaggttgac agtgttgcgg cttcgctgtt tttagaaact
     1381 tggcttgacc agcaaaatcg agtaaatgcc acgagtaaat cgagtaaaag gacggagtaa
     1441 atcgagcgtg ggccccgggg aaatgaagaa aatagcggtg attgatggga attcgtggac
     1501 gcatcgtgca tatcatgcgg tgcctcctgt gatgaagttg gctgatggca cgccaacgaa
     1561 tgcggttcac ggatttatga atatgtttct gagcttttat gaaaaagctc agccagatgc
     1621 tgtaatttgc gcctttgatg ctggaattcc gcaatttcgc ttagaagcgg ttgagcagta
     1681 taaggctcag cgaaagccga tggacaatga acttcgtgtg caatttccaa tcattgaaga
     1741 gatgcttgag agcatgggga ttccagttgt taaggttgaa ggttgggaag gcgacgatgt
     1801 attaggcacc attgctgata agtgcgaaaa gctcgggttt gaaactttgc ttgtgacagg
     1861 tgataaggat gctaatcaac ttgcgactga tttaactaaa attgtaaata tgaaaaaggg
     1921 cgttagcgat gttgccattc ttggtcccgc cgaagtcaaa gaaaaatacg gagttagcgt
     1981 tgatcaattt atagattacc ttgcactgat gggggattct tcagataata ttcctggcgt
     2041 tcctggaatt ggacccaaga gtgcgtcagc gcttttacaa aaatatgaaa gccttgaagg
     2101 gctttatgaa catattgacg agcttaaagg aaagcagcaa gataacattc gtgacaataa
     2161 agacgctgct tatgttagta ggactgctgc gacaatttcg aaagatgtgc caattgatat
     2221 tgatttggag aacattgcgt ttcccaactt cgatcaagac acagtaagtg aaacgttttt
     2281 aaagttccgc atgcgttctg ccttgagcgg agttttaaaa ttgattggag tagttccgga
     2341 agaatcttcg cctgaagatt cgaaggcgga agcttcgcaa ccaagacctg caatcaaaaa
     2401 agaagaaaaa gttgtactta aagaaccgaa actcgtcttt gaaaattatg ttgaaggcga
     2461 agatgcactt gccctgtcca agaaattaat taaaggtgat tttgccattg cgatttctca
     2521 taagcaggaa gcacctgttc aggcttcgct ttttgacgag gaaattgcct caaaatatcc
     2581 tgagtataag aatttattat tgggaatctc aaacaacaaa gagacagcaa tcctcgaagg
     2641 cgactccgct attgaaactt tagttgagat tacaaaatac aatcatgaag tgattgctga
     2701 aaatgctaaa gcaatttttg agatactctt cgacgagttt attggcgcag atgattttta
     2761 taaattaaat ttttatgatg ttagcctcgc tgcgtatttg ctaaattctt ctaagagtga
     2821 atatagtgct gttgatgcat ggcaacaagt cttcggaagt ttgcttccga cattagatat
     2881 tgaacctgag gaaggcgaaa agaaaactga aaaatctttt atagataaaa agaataagat
     2941 tcttcagctt cgtcttgctc gccgtgcggc cgcttcgttt gcgttgagag agtttttaga
     3001 taagagatta agtgaagata atgcagataa aatttataag gacattgaaa ttccgctcac
     3061 aaaagttctt gttcaaatgc aggtaactgg cacaaagatt gaccgagcta tgctcgataa
     3121 gttaagtgtc aagacagcta aacttttgga acatttgaaa tctgaaattc ttgaacttgc
     3181 gggcgaagaa ttcaacatcg attctccaaa acagttaagt catattttgt ttgaagtttt
     3241 aggtttgcca gcgaagaaga aaaccaaaac tggctattcg actgatgctg aagttttggc
     3301 tgatttatcg gccatccatc cattgccggc taagattatt gaataccgcg aatacgcaaa
     3361 attaaaatca acctatatag atgcgcttcc agcattggca gacaagaatg atatagttca
     3421 tacaacttac aaccagaagg ttacaacaac cggtcgcttg tcttcaagtg atcctaatat
     3481 tcaaaacatt ccaactcgaa gtgagtttgg aaatcaaatc cgcgaatgct tcctgcctct
     3541 tcaaaaagac gaagtttttc taacggcaga ttatagccag atagaattac gtgttcttgc
     3601 tcatttatct caagacaaag gattaattga ggcttttaca agtggtaacg attttcatgc
     3661 tcacactgct gcaaacgttt ttggtgttag tgtagataaa gttacaccag acatgcgccg
     3721 taaagcgaag gcggtaaact tcggaattgt ttatggacag caggcgtttg ggctttcgca
     3781 aagtttaaag attagtatgg gcgaagcgaa gcagatgatc gacagttact ttagtagtta
     3841 tcctcaagtt cgtaagtatt tagatagggt tgtcgaagac gcaacgctca aaggttatgc
     3901 cgaaacgatg ttcggccgac gtcgttacat tccagagttg actgcaacga acaagcgctt
     3961 ccgtggcttc ggagagagaa ctgcaatgaa tcatccaatg cagggcagcg ccgccgacat
     4021 tattaaaatc gccatgattc gcgttagtga ttctatacgc aagcaaagtt tgaaatcgaa
     4081 acttatgttg caggttcacg acgaattgga tttgtcggta gataaatctg aaattgatac
     4141 tgtaaccgag ttggtaaaga ctgaaatgga aaacgcagtc aagttagaag ttccgctagt
     4201 tgtggatatt gcgacaggag aaaattgagc gaaggcagaa taacaggttt aagttccgtt
     4261 gaggttgagg agagagttaa ggctggcaaa gtcaataccg atgctggctt gaagactcgt
     4321 tcgattaagc gaatttgcta cgacaacatt ttcactttgt tcaatgctgt caacgtgatc
     4381 cttgcgatta ttgtcgcact tacaggcagc ttcaaaaaca tgcttttcat gggcgttatt
     4441 gtttgcaata cagcaattgg aatcatccag gaaattcgaa gcaagaagac aactgacaag
     4501 ttaactattg ttgcaaaagc gaaggttaag gcgcttcgtg atgggaagac tgtcgagatt
     4561 aatagtgacg aaattgttga ggacgatatt attgagcttg ggcgcgggga tcaagttccg
     4621 gcggattgta ttgttgttga aggtttttgt gattgcaacg aaagcttgct tactggagag
     4681 agcgatctta tcgcaaagca aaaagacagt aagctcatga gtggatcttt catcaacagc
     4741 ggcaaagttt atgcaaaggt aacgcacgtt ggagctgcaa gttatgctaa taaaattacc
     4801 gccgaagcca aggtccataa gatggttaat tctcaaatta tggaaaccat gaatggactt
     4861 attcgcatta tttcaattat cattttcccg cttggtgcgc tgcttttcgc gaaagcttat
     4921 ttcttcaacg gaatcgattt aatccaatct attcttacaa cggtttcggc aatgataggt
     4981 atgatccctg agggacttat tttgctgacg tcaacagttt tggcggttgc ggttgtgcgg
     5041 ttggcgaagc acaaggtttt ggttcaacag ctttattgca ttgaaacatt ggctagagtt
     5101 gacgtttttt gtgcggataa aactgggact attacttcgg ggaaaatgct gttagataaa
     5161 attgttccta taaagaagga tagcgaagac gagcttggcc gtgctatcaa aaatatcaac
     5221 gatgccgatg acgacaaaaa cgaaaccgcg cttgcgattg aaaattattt caagggacgc
     5281 aaggctgaaa agttgagctt gaagaaggtc attccttttt cctcggataa aaaatgaagc
     5341 ggtgctgaat ttagtgaagg ttgctatgtt caaggtgcga cgcagtttat tttaaatgcg
     5401 agcgaaggcg cggttgttaa gcaagctcaa gatttggctg ttgattcgcg agttttgctt
     5461 gttgcaaaag ttgatggctt tgatgataaa ggcaacatta agggcaagcc taaaccgctt
     5521 ggatttattt gcattaagga tgaaattcgt gacaccgctg ctgataccat taagtttttc
     5581 caggacgaag gcgtaaccgt caaggtaatt tctggcgatg accctcgcac ggtttctgga
     5641 attgcgaaaa ctgttggtgt tgcgaatgct gaaaaatatg tggacgcaac cactctaaaa
     5701 accgacgaag aaattgccga agcggtccag aacttcgctg tttttggaag ggtaaagcct
     5761 gaacagaaga aagcttttgt cattgcgctt caaaaatttg gccacacagt tgccatgact
     5821 ggcgatggcg ttaacgatgt tttgagtttg aagcaagcgg attgcagcat cgcaatggca
     5881 tcggggagtg cggctgccag aaatgttgca cacttggttt tgctcgagaa tgattttgca
     5941 acaatgacaa atgttgttgc cgaaggcagg cgaagcataa acaacctgca gcgctcagct
     6001 tcgttatttt tagttaaaac attactttca atgtctcttg ctttgatttt cttattcatg
     6061 ccttggggtt acccattcca gccaattcaa atgacgctga tttctgcgtt tactattggt
     6121 gtgccatcat tcgtgctggc cttggagcca aatcacgaca ggattaaagg gcattttttg
     6181 accaatgtta ttgtgaggtc tataccgtca gccgctgtaa tagtgattgc aattttgttt
     6241 ataaatatta ttggctacaa ctttttcaat tttgattacg gccaaatttc aacaatgtgc
     6301 gtgcttacaa tgtcaattat tggctttatg ttggtaattc gtttatcgat tccgtttacg
     6361 ccaattcgca tcggactgat tattgtgatt gttggtggca caattttggg ttgcgtcttc
     6421 ttcggcaatt tgtttaacat cgcagaatac acaacagaaa tggctttgtt ccatgcgata
     6481 acttgcgcag tggggtgtgg gcttttcttc gttttttata agatcgtaga catttgatac
     6541 aataaatatc aatcaaaatt cgtataaaag atgcatttca tgcctggcaa taattacacc
     6601 ttttggtgca tttcatgcct ggcaataatt acaccttttg tggtataata cgtgtgtatg
     6661 aaaatgaagc ttaaacgtag catgaaagta gcaattgcat gcgtggttgt tctcacgctt
     6721 gcattgtctt ctttatttat ttccagaaat cataaaaccg ctaatgctga cccttcggct
     6781 gttagcaatt atatagaagc gaaggcttat ctttccagcg ttcgtggcga atacaatgca
     6841 attatgtcgg aagctacttc aatatacaac cagattgaag caacaaccaa aaaagctttt
     6901 gaagctcaaa gggcaatgac cgaatctcag cttcagctaa attcgttgct aaaatatcaa
     6961 tacgaaaact caacgatctt ttctattttt attatggttg catgttctac aagccttgat
     7021 gacttttcga aaaacattga ttatgcaaat tctgcaatgg attacctttt caacgtttca
     7081 aaagacaaca aagaaaagaa ggatgctttt gacagcacgc ttcaaaattt aaactctcaa
     7141 aatgctgctc aaaacgcatg cttggcttct gcttcggaga agttagccga agctgaaact
     7201 gtcttgcaag gtattcgcgc taagttaacc cccgaagaac ttgccgaact cgaaggagag
     7261 atatccgaca tcggaggcgg cggcggaggc ggcggcggag gtgacactcc aacgcctgga
     7321 ccaacacctc cgggaccaca accaggccca tctccagacc ctccaacacc aggctggtcc
     7381 agtgggcttg caagtgcgta tggtggatct tcggaccctt atacgccaaa ccctggaaca
     7441 actgcaactg gcgaagtttg cgatgactgg tcaactggag ttgcggtccc tatggcttga
     7501 ccaagttact ggaattattt tcatcacact gttcaaattt catgaaatgg catggtggtc
     7561 ctcgcaaaaa ttaacgattg cggaggtatg ggcggaggaa gtcgaagctt agacttgcaa
     7621 cctggcgttt ttaaagcctt cggatttcaa acatgtcagg cttgaggagt tcgccaggtt
     7681 gactatttga taatgtagta atttgttata atggaggata gatgaggaat ttatttcaaa
     7741 aacataaatg actctatgcg ttaactgcaa tcctaatttt tgtgattgta attggcatct
     7801 tgctctattg cgtttactat tttaagtatc gagcaacatc tttggcctgt cagtatgcgc
     7861 ttaaaggtta tataactgct gatgagatgg atgaaatacg acgttcgggc gacattgttt
     7921 taggcgaaga aattgaagat cgagaaggaa atattgttgt ccttcagttg aatgacaagg
     7981 gtgaatatgt tgcttatgta gttcgtgatg ctaacggaga tgagcctcac actcatgttt
     8041 gagaggcgca tcgaactaca catttggttg aggttggctc aaaatttgtt gctgacggag
     8101 taaataacgg cgaagaatat gggcactatg aaccaatcta cgaagaacag ccctacacag
     8161 attactacta ttgctctgaa tgtggagttc ataagtaatt tttggtttaa tcttggttta
     8221 atgtgttaga ctaacggcta ataattagct gagatggagg aggaataatg gccaaatttg
     8281 ttgacggcga atctcatact tttgatgagt atcttttaat cccgcgtcat acgagcgaga
     8341 aatgcacacc tgacagagtt agtttaaaga ctccgcttgt aaaatttaag aaggggacta
     8401 agccttcgct gtcaatgaat attcctatgg tttcggccat tatgcaggcg gtttctggtg
     8461 accgcttagc tgttgctctt gcgaaggagg gtggcgtttc ctttatttat ggatctcaac
     8521 caatcgagaa gcaagctgaa atggtttatc gcgcgaagcg agttaaggcg ggcttcgttg
     8581 atagcgattc aaacttaaaa ccagatgaca ctttggctga tgttttagcc ttgaaggaaa
     8641 aaacaggtca ttcgactgtt gcgatcaccg aagacggaac ttcggcgggt aaacttgttg
     8701 gcattgtcac tggtcgcgat tatagaatta gtcgtatgaa gccagatact aaggttaaat
     8761 cctttatgac gcctaaagag aaacttattt atgcgaagga tggtgctact cttagcgaat
     8821 gcaatgacat aatttgagat cataagctaa attcgcttcc aattcttgac aaaaagggtc
     8881 gtcttactgg catggttttc cgcaaagatt atgagagcca taaggaacat cctgatgagc
     8941 ttttggataa tacgaagcgc tatattgttg gtgcaggaat taattctcgc gattataagg
     9001 acagaatccc tgctcttgtc gaagctggag ctgatgtttt gtgtattgat tc
//
LOCUS       DBNCDC010000040         9021 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_3484_length_9021_cov_6.839059, whole genome shotgun sequence.
ACCESSION   DBNCDC010000040 DBNCDC010000000
VERSION     DBNCDC010000040.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 9021)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9021)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9021
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3484_length_9021_cov_6.839059"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="AC04VQ_04065"
     CDS             <1..356
                     /locus_tag="AC04VQ_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HIO9553346.1"
                     /translation="RGGVASALNEFAAQSNVDITINEVEVPVRREVKSACEMLGYDVF
                     QVANEGKIIAIVPPDQADAALSAMRANKYGKESAIIGYVDEAASTTPKVYVQTEFKTR
                     RILDKLVGEQLPRIC"
     gene            430..777
                     /gene="rplS"
                     /locus_tag="AC04VQ_04070"
     CDS             430..777
                     /gene="rplS"
                     /locus_tag="AC04VQ_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIO9553347.1"
                     /translation="MDIINALELQQKKEGLPEFKVGDNVAVHYRIVEGNRERIQVFQG
                     DVIARKGSGSRETFTVRKISFAVGVERTFPLHSPKIEKIEVTRHGDVHRAKLYYLRDK
                     VGKAAKVKEKSLV"
     gene            1039..1605
                     /locus_tag="AC04VQ_04075"
     CDS             1039..1605
                     /locus_tag="AC04VQ_04075"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIO9553348.1"
                     /translation="MLGLDEVGRGPLAGPLTVGGVILKSKPLVLGLNDSKKLSPKKRE
                     EIAEDIKKNSICAKTFSVPPKYIDDLGMTECLKRAFRNVVKQIEDEGIKIDVILLDGN
                     PLSFDPREVNVVKGDAKCASISAASIIAKVERDSYMEKISPDYPQYNFAQNKGYGTAE
                     HIEAIKQNGLSAVHRKSFCGSFMQMSFF"
     gene            1759..1884
                     /locus_tag="AC04VQ_04080"
     CDS             1759..1884
                     /locus_tag="AC04VQ_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553349.1"
                     /translation="MSAEETEVQLETSHNKSLGAKGEEAAARYLEGKAYEIIDRN"
     gene            1897..2157
                     /locus_tag="AC04VQ_04085"
                     /pseudo
     CDS             1897..2157
                     /locus_tag="AC04VQ_04085"
                     /inference="COORDINATES: protein motif:HMM:NF014119.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
     gene            2157..3416
                     /locus_tag="AC04VQ_04090"
     CDS             2157..3416
                     /locus_tag="AC04VQ_04090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIO9553350.1"
                     /translation="MQEISYGRYSLLSATLRGVEAVPVEVEVSVFRGLPSFSIVGMPD
                     SSIQESKERIFSAIKSSKFIMPNARIVVNLAPGHIKKSGSGFDLPIAVAILVATGQIP
                     YDEIYKYMLIGELSLDGAVREIPGLLAFALCAKDKGLNLLGADSKEQSVEVEGVNQFY
                     VESLSSFRENDFKPVEFVNNNDQDYECYDFKDISGHDVAKRALQIAACGNHGVLMIGP
                     PGSGKTMLASRLPSILPPLTKDEQMESAVIHSIAGESVTSILDGVRPFRSPHHSATLP
                     GLIGGGNPTRPGEVSLAHNGVLYLDELAEFGTHVLQGVRQPMESGDVTIIRADGAVTF
                     PARFSLIAASNPCPCGYFGDKEHECKCTAKQISNYQRKIGGPVMDRIDMHLDVSRIDA
                     KSVLNTGNGTSSDELLQGVLKGRDFAH"
     gene            3447..3677
                     /locus_tag="AC04VQ_04095"
     CDS             3447..3677
                     /locus_tag="AC04VQ_04095"
                     /inference="COORDINATES: protein motif:HMM:NF024731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase subunit ChlI family protein"
                     /protein_id="HIO9553351.1"
                     /translation="MEALVESCKLSSDDFEFFEEVATDNNMSGRGIMRVLSVARTIAD
                     IDTSENVTHEHLCEALTFRVRDKNTSMIEQLC"
     gene            3671..4531
                     /gene="dprA"
                     /locus_tag="AC04VQ_04100"
                     /pseudo
     CDS             3671..4531
                     /gene="dprA"
                     /locus_tag="AC04VQ_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014533.8"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
     gene            4636..5979
                     /gene="trmFO"
                     /locus_tag="AC04VQ_04105"
                     /pseudo
     CDS             4636..5979
                     /gene="trmFO"
                     /locus_tag="AC04VQ_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013314.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
     gene            5964..6935
                     /locus_tag="AC04VQ_04110"
     CDS             5964..6935
                     /locus_tag="AC04VQ_04110"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HIO9553352.1"
                     /translation="MEENLEKIEKFEFSTDECKAFSILVDKFCESLTVEKNSSEHTVR
                     NYKNDLLSFAIWCERMQLDPCMANYRELRGYLAEMDGAKYARTTINRRLSSLRSFFKW
                     LDVIGEETNNFADVMKGPKILKTLPHVVRHNDIENLMKVCMDEIEASESDVDRAKAKR
                     DYAIMELFYATGARISEVANLKLENINFESGQIKFFGKGMKERIVPIYNIAASALADY
                     IENSRGMLLRGNSSDICFIGNSGRPMSADSIRKMFYKKCEAAGVSSEISPHAMRHTFA
                     TDVLDGGADLRSVQEMLGHASLSTTQIYTHLSAKKLKDVHAQAHPRG"
     gene            6994..7338
                     /locus_tag="AC04VQ_04115"
     CDS             6994..7338
                     /locus_tag="AC04VQ_04115"
                     /inference="COORDINATES: protein motif:HMM:NF015721.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIO9553353.1"
                     /translation="MQNENVNIVSPNEIASVVRQACSDCYGIVGMASPNGITLLTNIL
                     PPILNRQGVEVIYTSEGYKINIYIVAEYGTSFEAIAKSLIDTVKYYLFDSLGVKTNGI
                     RVHIKGVRKSEI"
     gene            7338..>9021
                     /locus_tag="AC04VQ_04120"
     CDS             7338..>9021
                     /locus_tag="AC04VQ_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009608371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HIO9553354.1"
                     /translation="MVSKNSDMQNMTKNLNDEGMHYSRKPFITALIHASEMLSEYKEE
                     INKLNVFPVPDGDTGTNMSLTLESVVEHVKKLGDNPSLADLRRAVTTGALMGARGNSG
                     VITSQILRGLCEGYEQHELFDTQGLSIAFSRAEEVAFAAVRKPVDGTILTVLHDSAKA
                     ARYAQRKKMNIAEAISYVSEKAHESVENTPNLLPVLKENNVVDAGGYGLAIFIEGIAS
                     GLTGHDGAISEELKHNVGDISQPKVEIEQINDWAGSKYRYCNEFLVDSKTLDTEDALK
                     FLSSIGDCELCVGSTPKFKVHVHSDHPDKVLEYFIQRGQVSEVFIHNMQLQSEERVEK
                     LAEEAPHPQKDLAVVAVAVGSGNEEILKSLGVDVIVSGGQTMNPSTKDLADAAKKTNA
                     KNVIFLPNNKNIIMAAKSACDVLDINASVVETRNVLSAFSAMMVFDPQDSMENNISAM
                     NDSFSMLKVAEVTFAIKDSKDAHNNPIKEGDCIGIVDGNIEIVGKKIESVVMDLLKFL
                     DAKNMGMLTILAGEGIDDKLYSKLVLRIENKYDNLEIDHQEGGQPLYPILFSL"
BASE COUNT         2799 a         1422 c         1998 g         2802 t
ORIGIN      
        1 cgcgaggtgg tgtggcttcg gccctcaatg aatttgcagc tcaaagcaat gttgacatta
       61 caatcaacga agtcgaggtt cctgttcgtc gtgaagtcaa aagtgcgtgc gaaatgcttg
      121 gttatgatgt cttccaggtc gcaaacgagg gcaaaataat tgcaattgtc ccgcctgatc
      181 aggccgatgc agcgctttcg gctatgcggg ctaacaaata cggcaaggaa tctgcaatta
      241 ttggttatgt tgacgaagct gcttcgacaa cgcctaaagt ttacgttcaa acagagttta
      301 aaactcgccg aattcttgat aaattggtcg gcgaacaact cccacgtatt tgttaatttt
      361 ttgggtcaga ttcctgcttt ttggctataa tactcgcttg cacacacttt ttgggaagtg
      421 agggaaaaaa tggatattat aaatgcatta gaattgcaac agaaaaaaga gggcttacct
      481 gaatttaagg ttggcgataa cgttgctgta cattaccgta ttgttgaagg taatcgtgag
      541 cgtatccagg tatttcaagg cgacgtaatt gctcgcaagg gttctggttc tcgcgagact
      601 tttaccgttc gtaagatcag ctttgctgtt ggcgttgagc gtactttccc acttcattct
      661 ccaaaaattg agaaaattga agttacccgt catggtgatg ttcatcgtgc taagttatat
      721 tatcttcgtg ataaggtagg taaggctgct aaagttaaag aaaagtcttt ggtttaattg
      781 gtagttttgc caaataaaga aagcttaaag tccatgtctg tttctcaggc acggactttt
      841 tttatggata atgctaagga gtttagttca actgacattt caatgctaac tgaacttgtt
      901 aaaagcgatg gcagatcagg tgttcttgat gcttgaaata agtttttaaa aactaaaagc
      961 gatgaaaagg ccgaagtcga gcgcgtttct aaactttatg attttgaaaa gacaattgct
     1021 gctcttcctg gcagtgtaat tttaggactt gacgaagtcg gacgtggccc gttggcaggt
     1081 ccattaactg ttggtggagt aattctaaag tctaaacctt tagttttggg tttaaatgac
     1141 tcaaaaaagc taagccctaa aaaacgcgaa gaaattgctg aagacattaa gaagaattcc
     1201 atatgtgcaa aaacattttc tgttccacca aaatacattg atgatctggg catgactgaa
     1261 tgtttaaagc gagcttttcg aaatgttgtt aaacaaattg aagacgaagg aattaagatt
     1321 gatgtaattt tgctggatgg aaaccctctt tcttttgatc cccgtgaagt taatgttgtt
     1381 aagggagatg cgaagtgcgc atctatctct gcggcatcaa taattgctaa agttgaacgt
     1441 gattcataca tggaaaaaat tagtccagat tatccacaat acaattttgc acagaacaaa
     1501 ggatatggaa cagccgagca tatagaggct atcaagcaaa atggtttatc ggcagtacat
     1561 cggaaatcgt tttgcggttc atttatgcaa atgtcttttt tctaaattat tatttatatt
     1621 taatattttt tattcaaatt tatttttatt taaaaatttt tagcgagcgt taattattgt
     1681 tcactctcgt ttcagccact cttctgcgcc agtttatcgt ttgtgagtaa aatgcacagc
     1741 tatattgaaa ggagagaaat gagtgctgaa gaaactgaag tacaacttga gacaagtcac
     1801 aataaatctt tgggtgcaaa aggcgaagaa gctgctgcgc gttacctcga aggaaaagct
     1861 tatgaaatta ttgatagaaa ttgaaaatgt aagcttggcg aaattgacat tgtagcaatg
     1921 gataatggaa ccatagtctt tattgaggtt aagacacgtg caaatttgga aaaaggtctt
     1981 cctgaagatg ctgtaggtcc aaaaaaacgc aagaaatatg agtgtttggc tgctatgtat
     2041 ttacaagatc atgattatgt tgacatgcca gtgcgtttcg atgttgttgg attgcttgtt
     2101 atgtcaaaaa acagggcatt tattcgccat cattacaatg cttttggagt tggctaatgc
     2161 aagagatatc ttatggaaga tattcattgt taagtgcaac tttgcgtggc gttgaggctg
     2221 ttcctgtcga ggtggaagtt tcggtgtttc gtggattgcc aagtttttca attgttggaa
     2281 tgcctgattc ttctattcaa gaatcgaagg aacgcatatt ttcggctatt aagtcttcga
     2341 agtttattat gccgaacgca cgaattgttg taaatcttgc tccaggtcat attaaaaaga
     2401 gtgggagcgg ttttgatttg ccgatagcgg tggcaatatt ggtggcaact ggacaaattc
     2461 cttatgatga gatttataag tatatgctta ttggtgaact ttctcttgac ggagcagttc
     2521 gagaaattcc cggactactt gcttttgctt tgtgtgccaa ggataaaggt ttgaacttac
     2581 ttggtgctga ttcaaaagaa caaagtgttg aggtagaagg tgtgaatcaa ttttatgttg
     2641 agtccttatc tagctttcgt gaaaatgatt ttaagcctgt tgagttcgtc aataacaatg
     2701 atcaagatta tgaatgttat gatttcaaag atatatctgg tcatgatgtt gccaagcgag
     2761 cattgcaaat tgcagcttgt gggaaccatg gtgttttaat gataggaccc ccaggatcag
     2821 gaaagaccat gttggcatca cggttgccat caatcttgcc accacttaca aaagatgaac
     2881 aaatggaaag tgctgtcatt cactcaattg caggggaaag cgttacaagc attttagatg
     2941 gggtgagacc tttcagatcc cctcatcata gcgccacttt gccaggttta attggaggag
     3001 gaaaccctac aaggccaggc gaggtttcac tcgctcataa cggagttcta tatttagatg
     3061 aattggctga atttggcact cacgttttgc aaggcgttcg tcaaccaatg gaatctggag
     3121 acgttacaat aattagagct gatggtgcag tcacttttcc tgcgaggttt tcgctaattg
     3181 cggcttccaa tccttgtcct tgtggatatt ttggagataa agaacatgaa tgcaagtgca
     3241 cggcaaagca aatttctaac taccaaagaa aaattggtgg tcctgttatg gataggatag
     3301 atatgcattt ggacgtttct cgaattgatg caaaatctgt tttaaataca ggtaatggga
     3361 cgagctcaga tgaactttta caaggagtgt taaaaggtcg tgattttgca cattgaaggc
     3421 taaaaggtga aaaaggccca aaaaatatgg aagctttagt tgagtcttgc aaattaagtt
     3481 ctgatgattt tgaatttttt gaggaagttg ccaccgataa taatatgagt ggacgaggaa
     3541 ttatgagggt gctttctgtc gcaagaacaa tcgcagatat tgacacttcg gaaaatgtaa
     3601 cgcatgagca tttatgcgaa gcgcttacat ttagagttcg tgataaaaac acttcgatga
     3661 ttgagcagct atgttaaagg ttaataacaa taaatttcta aggggcgatg cttttgagat
     3721 ttatccagaa gatgatgaat ttcctaatgc gttaaagatt ttaccggatg ctccaaaaaa
     3781 gttatatttg attggtaatc caaatgcgct taaggaaggc attggaattg taggggcaag
     3841 gcgtgcaacc ccttatggaa tatcttgcac gcgacatttt gctcgcctcg ccgctcgtca
     3901 aggaattgtt attatttctg gaggagctta cggttgcgat agcgcttcgc ataaagctgc
     3961 gttaaaagaa aataagcaaa ccgttgtatt tttaggcggt ggatgtaacg aaatttatcc
     4021 tgttgggaat gagcggatgt atcaagaagt aattgataaa aatggtgcga ttgtttctga
     4081 aaaagattga gattaccctc ccgtgccaca ttcttttctt gccagaaata ggcttatagc
     4141 aggtctttcg aaagctttgt tgattgttga agctgggctt ccatccggga cattttctac
     4201 agctgactat gcaataaaat ataataaaga agttttgtct gtgccagggc caattacaaa
     4261 cgacaattct tctggatgta acaagttaat ttatgatggc gcaaaaccaa ttatcaacga
     4321 tgaagttttt ttgaatgctt tgtttgaaat ttatggttgt cctctaaatc aagattataa
     4381 agatgccgtt aaagagatag aagaaaaccc acataagatt caagattcag aaaatcctat
     4441 tttatatgca ttgtgttcgc aagccatgtc tatggatgat ttatataaaa ttgcaaaaaa
     4501 ttattctaaa ggtaaaaatc caagtgtttg actttcggaa aaattagttg ctgctgaaaa
     4561 aactgggaga attgctaaat atccaaatgg aatgtatggt cctgtcctta aatcttaata
     4621 tataatttag ccttgatggc aaagtttaaa gttacaattg ttggcgcagg tcttgcaggt
     4681 agcgaggcag cgcttgtctt gtcaaaattc aacgatgttc aaattaaatt aattgagatg
     4741 cgaccaaagc aaatgaccga ggttcacaaa accggagatt gcgctgaact tgtttgctca
     4801 aattcgttta aatcgctaaa gccagatagc gctgcaggaa tgttgaagtt cgaactcgag
     4861 gccttggctt cgccggttta tgattgtgcg ataaaaaata agattgaagc cggaaatgct
     4921 ttggcagttg atagagataa gttttctaaa agtgttagtg agaaaattgc gtctcaaaaa
     4981 aatattgaac ttgttgttga tgaatttgtt gacattaaaa gtgaatctaa aaattgcgat
     5041 gctcttgtcc tttgcactgg tcctcttaca agcgacaagt tagcgaataa tttgcaaggt
     5101 ttaactggtg atgatgaact tgcattttat gatgcagctg ctcctattat tatggatgac
     5161 tcaattgata agtctattgt gttttcgcaa gataggtatg acagtgaaaa tgttggcgct
     5221 tatttaaatg ctccaatgac aaaagatgaa tatgaaaatt ttatcaatga actgcttgat
     5281 gctgaatgtg taattaaaaa ggattttgaa tcgaaggatt tgtttcaagc gtgtcaacct
     5341 gtcgaagaag ttgcacgaag cggatttgac gctccacgct tcggtgcgat gaagcctatt
     5401 ggtttgagcg atccacggac cggaaaaaga ccttacgctg ttgttcaact tcgcgccgaa
     5461 aacgcaaaca agacagcatg aaatttagtt ggatttcaaa ctaacctaac atttcctgaa
     5521 caagagagag tttttcgaat gattccagga ttggaaaatg caaacttcgc tcgctttgga
     5581 gttatgcatc gaaacacttt tattaattcg ccaaaattgt tagacaaaga tttaaaactt
     5641 aaagataata tctatgttgc tggacaattg agtggtaccg aaggttattt ggaggctgtc
     5701 cgaagtggcc atcatgctgc aatttctatc ctcgctaatt tattaggaaa acaagctcct
     5761 gaagttccaa atgattctgt ttttggtgca ctcattgatt atgcaacaaa cgaagaaacc
     5821 gtaaactatc aaccaatgca tgtaaatttt ggaattttgc ctcctcttga aaagcaaata
     5881 aaaaataaaa ggcaacgcta cgaagaattt gcgaagcgtg gtagaattgc aattgaaagt
     5941 tatgctaaag aagtaaatga actatggaag aaaatttaga gaaaatagaa aagtttgaat
     6001 ttagcacaga tgagtgtaaa gctttttcaa ttttggtcga taagttttgt gaatctttaa
     6061 ctgtcgaaaa aaattcttcg gagcacacag ttcggaatta taaaaatgat ttattaagtt
     6121 ttgcaatttg gtgcgaacgc atgcagcttg acccttgcat ggcaaattat cgtgagcttc
     6181 gtgggtattt agcagagatg gatggtgcta aatatgctcg gacaacaatt aatagaaggt
     6241 tgagttcgct tcggagcttt tttaagtggc ttgatgtaat tggcgaagaa actaataatt
     6301 ttgctgatgt tatgaagggt ccaaaaattt taaagacttt acctcatgtg gttcgccaca
     6361 atgacattga aaatttaatg aaggtttgta tggatgagat tgaggcgagc gaaagcgacg
     6421 ttgatagagc aaaagctaaa cgtgattatg cgatcatgga acttttctac gcaactggtg
     6481 cacgtattag cgaagttgct aatttaaaac ttgaaaacat taattttgaa tccggccaaa
     6541 tcaaattttt tggaaaaggt atgaaggagc gtattgttcc aatctataac attgctgcaa
     6601 gtgctttggc tgactatatt gaaaactctc gtggaatgct tttgcgaggc aacagttctg
     6661 atatttgttt tataggaaac agcggtcgtc caatgtctgc tgatagcatt cgaaaaatgt
     6721 tttataaaaa atgcgaagct gcgggtgtgt cttcggagat ttctccacat gcaatgaggc
     6781 atacttttgc gactgatgtt ttggacggcg gcgctgatct tcgtagcgtt caagaaatgt
     6841 tgggtcatgc aagtttgtct accactcaaa tttacacgca tttgtctgca aaaaaattaa
     6901 aagatgtgca cgctcaagct catcctcgcg gataaaaatt atcgcgtttg agcactaaat
     6961 ttaaggtaaa atatttaagg ttttgaggta ataatgcaaa acgaaaatgt aaatattgtt
     7021 agccctaatg aaatagcgag cgttgttcgt caagcttgct ctgattgcta tggaattgtt
     7081 ggtatggcaa gtcctaacgg cattacttta ttaacaaata ttttgcctcc aattttgaac
     7141 agacagggtg ttgaagtaat ctatacaagt gaaggttata aaattaatat ttatattgtt
     7201 gctgaatacg gcacaagttt tgaggcaata gcaaaatcgt taattgatac tgtcaagtat
     7261 tatctttttg attccttagg agtaaaaact aacggaattc gagttcatat taaaggtgtt
     7321 cgcaaaagtg agatttaatg gtttctaaaa attctgatat gcaaaatatg actaagaatt
     7381 taaatgatga aggcatgcat tactcaagaa agccttttat cactgcgttg attcatgctt
     7441 ctgaaatgct ttcggaatat aaagaagaaa tcaacaagct aaatgttttt cctgttcctg
     7501 atggagatac gggaacaaat atgtctttga ctcttgaaag tgttgttgag catgttaaaa
     7561 agttgggtga caatccttcg cttgctgact taagacgagc tgttacaacg ggagcgctca
     7621 tgggtgctcg tggaaattct ggagttatta cttcgcaaat tttgcgagga ctttgcgaag
     7681 gctatgagca acatgaactt tttgacactc aaggattgtc aattgcattt tcgcgcgccg
     7741 aagaagtagc attcgctgct gttagaaaac ctgtcgatgg aacaatattg actgttcttc
     7801 atgattcggc aaaagctgct cgttatgctc aacgtaaaaa aatgaatatt gcagaagcta
     7861 tttcttatgt ttctgaaaaa gctcatgaat ctgtcgagaa tacaccaaat ttgttgccag
     7921 ttttaaaaga aaataatgtt gtggatgccg ggggctatgg cctcgcaatt tttattgagg
     7981 gaattgcaag cggtttgact ggtcatgatg gtgcaataag cgaagagctt aaacacaatg
     8041 ttggtgacat atctcagcca aaagttgaaa ttgaacaaat taatgattgg gcaggttcca
     8101 aatatagata ttgtaatgag tttttagttg attcaaaaac tctagacacc gaggatgctt
     8161 taaagttttt gtcaagcatt ggagattgtg aactttgtgt tggaagtact ccaaagttta
     8221 aagttcatgt tcattccgat catcccgata aggttttgga atatttcatt cagcgtggtc
     8281 aagtttctga agtttttatt cataacatgc aacttcaaag tgaagagcgt gttgaaaaat
     8341 tagcggaaga agctccacat ccgcaaaagg atttagcggt tgttgctgtg gctgtaggtt
     8401 ctggcaacga agaaattcta aaaagtcttg gtgttgatgt aattgttagt ggcggacaaa
     8461 ctatgaatcc ttcaactaaa gatttggccg atgctgcaaa aaagactaac gctaaaaatg
     8521 taattttctt gcctaacaat aaaaatatta tcatggcagc aaaaagtgct tgtgatgttt
     8581 tagacattaa cgcaagtgtt gttgagacta gaaatgttct tagtgctttt agcgcgatga
     8641 tggtatttga cccgcaagat tcaatggaaa ataatatctc agcaatgaat gattcttttt
     8701 caatgttgaa ggttgcagaa gttaccttcg ctattaaaga ttctaaagat gctcacaaca
     8761 atcctatcaa agaaggcgac tgcattggaa ttgttgatgg aaacattgaa attgttggca
     8821 agaaaattga atcagttgtt atggatttgt tgaaattctt agatgcgaaa aatatgggta
     8881 tgctcacaat tttagccggt gaaggtattg atgataagtt atattcaaaa ttagttctgc
     8941 gcattgaaaa taaatatgac aatttagaaa ttgatcatca agaaggtggg caacctctct
     9001 atccaattct cttttcattg g
//
LOCUS       DBNCDC010000041         8625 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_3795_length_8625_cov_7.964294, whole genome shotgun sequence.
ACCESSION   DBNCDC010000041 DBNCDC010000000
VERSION     DBNCDC010000041.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 8625)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8625)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8625
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3795_length_8625_cov_7.964294"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(38..304)
                     /locus_tag="AC04VQ_04125"
     CDS             complement(38..304)
                     /locus_tag="AC04VQ_04125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014838.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta/epsilon"
                     /protein_id="HIO9553355.1"
                     /translation="MAKLNVEIVTPSSKVFTGEAYMVTVPGLAGSLGFLPGHVGLVTP
                     LGKGKVSISNSDKSDDASAKHFNIEGGYVQLFDDNVVVLAEHVE"
     gene            complement(308..1753)
                     /gene="atpD"
                     /locus_tag="AC04VQ_04130"
     CDS             complement(308..1753)
                     /gene="atpD"
                     /locus_tag="AC04VQ_04130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015778753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIO9553356.1"
                     /translation="MASKTKKKTASVGAKGTIVRIVGAVVDVEFDPDKMPAIYNALHV
                     EADTAAGHIDAILEVQTHLRGDMVRCVAMTSTDGLVRGLEVVDTGAPIQMPVGDETLG
                     RIWNVLGEPVDGKPMPKISDRYPIHHEPPAYDTLTTETEIFETGIKVIDLVEPYVKGG
                     KTGLFGGAGVGKTVCIQELINNLAQEHGGTSVFTGIGERTREGTDLFLEMSESGVIDK
                     TCLVYGQMNEPPGARLRVGLAGLTQAEYFRDKGQDVLLFIDNIFRFTQAGSEVSALLG
                     RMPSAVGYQPTLATEMGDIQERITSTDVGSITSVQAVYVPADDLTDPAPATTFTHLDA
                     KTVLSRSIAELGIYPAVDPLESSSTALDPEIVGEEHYRVATGVQEILQSYKNLQDIIA
                     ILGMDELSEEQKLTVNRARKIQKFLGQPFHVAEVFTGNPGKYVKVEDTVRSFGEILDG
                     KCDHIPEQCFVYKGAIEDVYEAYEKMKAEEK"
     gene            complement(1765..2667)
                     /gene="atpG"
                     /locus_tag="AC04VQ_04135"
     CDS             complement(1765..2667)
                     /gene="atpG"
                     /locus_tag="AC04VQ_04135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015778754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIO9553357.1"
                     /translation="MANLKDIERRISSVKSTRQITSTMQMIAAAKVAKSASRLEESMP
                     YTSAISNILVSVSKGTSDVSHPLLSEHENKKTTLVIVVVSDKGLAGGFNSNILREAQH
                     ILNKNKKEGKDTKVIACGKKAKGYFEFRNIPLQMEFSGSSDNPQFEQAEEIGNYCIDG
                     YTNGEIDEVILVFNKCKNSMEQTVENKKILPCTAQDLVEAAEETAKQEDGEYLKEIVY
                     EPDTQSVLESLIPTYIRNMIFNALLDSAAGEQVARRVAMQAATDNADEMTETLTRLYN
                     SVRQGAITTELNEIVGGAEALAAD"
     gene            complement(2671..4284)
                     /gene="atpA"
                     /locus_tag="AC04VQ_04140"
     CDS             complement(2671..4284)
                     /gene="atpA"
                     /locus_tag="AC04VQ_04140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012799252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIO9553358.1"
                     /translation="MAKKEADVKNLTAESIANNLKAQIEEISTVVMSKEQGTVIQVGD
                     GIARVYGLAGAMMGELLEFVSSDGESVYGLVQNLEDEEVGAVLLANVTKIKENDLVKT
                     TGRVLEVPVGDGMLGRVVDPLGNPIDGKGPIKISGHRPIEFKAPGVIDRKPVHEPVQT
                     GLLSVDSMIPIGRGQRELIIGDRQTGKSAIAIDTIINQKDEDMICVYVAIGQKASSVA
                     RTADILAKHGALDHTIIVSANASDSAPLQYIAPMAGAAIGEYFMYNGADGKPANAKNP
                     GRHVLCVYDDLSKQAVAYRQMSLTLRRPPGREAYPGDIFYLHSRLLERAVKLSDEHGA
                     GSLTALPIIETQAGDVSAYIPTNVISITDGQIYLQTDLFFQGQRPAVDVGISVSRVGG
                     NAQIKAMKQVAGNLRLDLASYRELQAFTQFGSDLDKATQQQLNRGSHMTQLLVQGRYM
                     PIPVELQACVIYAANKGHIDDIEIEEVLAYRDELMKYLETSGKKILDTLKKEKKFDEK
                     LEAELDKQLDAFDKKFKKNMPKKEEGAEE"
     gene            complement(4313..4906)
                     /locus_tag="AC04VQ_04145"
     CDS             complement(4313..4906)
                     /locus_tag="AC04VQ_04145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HIO9553359.1"
                     /translation="MKDDRSTRRKKQQAYAESILLNAVLIDGIDEALRVLDDLNNILK
                     ATMGNVQLADVFRDQVDEYERESRKKIDSGKLVREIFSGASEAVLDTLSVMAENRDLY
                     LCMSMYETYNKLLEEHFDVIVVNVVSAVELDDHLRELIKTKAQNELDANIVLNEHVDK
                     NILGGVILSARNRIVDCSLLTMMERAKSELTSAHHYG"
     gene            complement(4910..5422)
                     /gene="atpF"
                     /locus_tag="AC04VQ_04150"
     CDS             complement(4910..5422)
                     /gene="atpF"
                     /locus_tag="AC04VQ_04150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIO9553360.1"
                     /translation="MESSGIQVILPNMIEFIPMLIAFIIVAIVLWKFGWPKFEGMLET
                     RKEKIEGALKDSEEKKLEAEKLLEENKKMLEEAESQADEILKEAKLSSSEIGKNIEEA
                     AKRQAESTTEKAKLAINQQKKAAEQDIKIQAVDIAMSAVKKFVSADLDDKEHRKLIEK
                     YVKEAGSLKA"
     gene            complement(5454..5666)
                     /gene="atpE"
                     /locus_tag="AC04VQ_04155"
     CDS             complement(5454..5666)
                     /gene="atpE"
                     /locus_tag="AC04VQ_04155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIO9553361.1"
                     /translation="MEIAAGLKVIGYGLAVIGPALGIGLACYGACMATARQPELQGRL
                     FTNFIIGAALVEALALIGFVLALIAS"
     gene            complement(5698..6486)
                     /gene="atpB"
                     /locus_tag="AC04VQ_04160"
                     /pseudo
     CDS             complement(5698..6486)
                     /gene="atpB"
                     /locus_tag="AC04VQ_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012347.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
     gene            complement(6486..6812)
                     /locus_tag="AC04VQ_04165"
     CDS             complement(6486..6812)
                     /locus_tag="AC04VQ_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553362.1"
                     /translation="MFVFLGAVVGVLSFVPLLLSIKASKNVTKTSNFGYGALLILGVF
                     ASLIILVVAVLICYFVAKDGLLAFALAAAITLILFAIVYGIYTVIKRSKAAKERANRS
                     KKSKGK"
     gene            complement(6984..7181)
                     /locus_tag="AC04VQ_04170"
     CDS             complement(6984..7181)
                     /locus_tag="AC04VQ_04170"
                     /inference="COORDINATES: protein motif:HMM:NF016283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HIO9553363.1"
                     /translation="MCSEALGVGAAPDLLKLISLCNVCGINIKTFDSPEIYLHKDLAI
                     DFASWASPAFRTFCLLLMQKD"
     gene            complement(7507..8121)
                     /locus_tag="AC04VQ_04175"
     CDS             complement(7507..8121)
                     /locus_tag="AC04VQ_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HIO9553364.1"
                     /translation="MQNRKFKLRVHFKKVGLAAWTSQLELARALEHAIRRSGLPYAVS
                     EGFSPHMRISFAQALSVGIESEDNVFDILLTDYIAPEKCLAALKDASQSCLEPLSCAY
                     VDNKQAFEIEEVSKFSVNVACEIKDIKVPDKIKVVKKRKEKEFDVSKFLDGEVEHKSA
                     KGCTELSFSLKNFSDGSLNPEIFVRELLACSGLRDYQIINFKKL"
     gene            complement(8133..8483)
                     /locus_tag="AC04VQ_04180"
     CDS             complement(8133..8483)
                     /locus_tag="AC04VQ_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553365.1"
                     /translation="MLEAVISRGSRDVCNLIYEAWKRGAKFDAWDEFFNFDAWISAAD
                     KLGMNLQVLASQDLDGNLPWSHIDFGVDRKFFESEREKANAAKTTPDCTFDKCQNCGI
                     CQNLKVKNEIAGKR"
BASE COUNT         2662 a         2034 c         1394 g         2535 t
ORIGIN      
        1 aattgctcat tcgtttcatt tttcttttgt ttgaatctta ttcgacatgc tctgctaaaa
       61 caactacgtt gtcgtcaaac aattgaacgt agcctccctc aatgttgaaa tgttttgcag
      121 atgcatcgtc tgacttatca ctattcgaaa tactaacctt acccttaccc aaaggagtta
      181 caagtccaac gtgtcctggt aaaaatccaa gcgaaccggc gagtcctggc acggtaacca
      241 tataggcttc gccagtgaaa acttttgaac ttggagtaac tatttcaaca ttaagcttag
      301 ccatagacta tttttcctca gccttcatct tctcataagc ctcataaaca tcttcaatag
      361 cacccttata aacaaagcac tgctctggga tatgatcaca tttgccatcc aaaatctcac
      421 cgaaggaacg gaccgtgtct tctaccttta catacttgcc agggttacca gtaaatacct
      481 cagcaacatg gaaaggctgg cccaagaatt tttgaatctt ccttgcgcga ttaaccgtca
      541 acttctgttc ttcggagagc tcatccatac ccaaaattgc gataatgtct tgcaagtttt
      601 tgtatgattg caaaatttct tgaacaccag tagcaacacg atagtgctcc tcgccaacaa
      661 tctcaggatc aagagcagtc gatgaactct caagaggatc aacagcagga taaattccaa
      721 gctcagcaat agaacgagac aaaactgtct ttgcatccaa gtgagtaaat gttgttgccg
      781 gagcaggatc agtcaaatcg tctgccggaa cataaacagc ttgaaccgaa gtaatagaac
      841 caacatcagt tgaagtaatt cgctcttgaa tgtcacccat ctcagtagcc aaagttggct
      901 gataacctac agctgaaggc atacgaccaa ggagcgcaga tacttcggag ccagcttgag
      961 taaatctaaa aatgttatca ataaacaaaa gtacgtcttg acctttatca cggaaatatt
     1021 ccgcttgggt aagtcctgct aaaccaacgc gaagacgagc tccaggtggt tcgttcatct
     1081 gaccataaac aagacatgtc ttatcaataa caccgctctc gctcatctcg aggaacaaat
     1141 ctgtaccttc gcgagtacgc tctccaattc ctgtaaaaac ggaagttcca ccatgctctt
     1201 gtgcgaggtt attaattagc tcttgaatac aaactgtttt accaacacca gcaccgccga
     1261 aaagacctgt cttaccacct tttacgtatg gctcaaccaa atcgataacc ttaatgccgg
     1321 tctcaaaaat ttcagtttca gtagttagag tgtcataagc tggaggctca tgatgaattg
     1381 gatacctatc agaaatctta ggcattggct tgccatctac aggctcaccc aaaacattcc
     1441 aaatgcgacc taaagtttca tcgcctacag gcatctgaat tggagcgcca gtgtcaacaa
     1501 cttctaagcc gcggaccaaa ccatcggtcg aagtcatagc aacacaccta accatatcgc
     1561 cgcgaagatg cgtttgaact tccaaaatcg catcgatgtg accggctgca gtgtctgctt
     1621 cgacatgcaa agcattataa attgctggca tcttgtctgg atcaaattca acatccacaa
     1681 cagcgccaac aatacggacg atcgtcccct ttgcaccaac actagctgtc tttttcttcg
     1741 tcttacttgc cataataacc tccgttaatc agctgcgagg gcttcggccc ctccaactat
     1801 ttcattaagc tctgttgtaa tagcaccctg gcgaacgctg ttatacaatc gagttaaggt
     1861 ctcggtcatt tcatcagcgt tatcggttgc tgcctgcatg gcaacacgac gagcaacctg
     1921 ttcacctgct gcactatcca acaaagcatt aaaaatcata tttctaatat atgtaggtat
     1981 taaactttcc aaaacacttt gagtgtctgg ttcataaaca atttctttta agtattcgcc
     2041 atcttcttgc ttggccgttt cttcggcagc ctcaacaaga tcttgcgcag tgcatggcaa
     2101 aattttctta ttctcaacgg tttgttccat gctgttttta catttattga acaccaaaat
     2161 tacttcatcg atttcaccat ttgtatatcc atcaatacaa taatttccaa tttcttcagc
     2221 ttgctcaaac tgagggttgt ccgaagatcc agaaaattcc atctgaagcg gaatatttct
     2281 gaactcaaaa taacccttag cctttttacc gcaagcaata acttttgtat ccttgccttc
     2341 ctttttattt ttattaagga tgtgctgagc ctcgcgaaga atattcgaat tgaagccgcc
     2401 agccaaacct ttatcagaaa caacaacaat aactaaagtt gttttcttgt tttcatgttc
     2461 gctaagcaat ggatgcgaaa catcgctggt gcctttagat acgctaacta aaatattcga
     2521 tatcgcagaa gtgtaaggca tggactcttc taaacgcgaa gcactcttcg ccaccttcgc
     2581 agcggcaatc atttgcatag tgctcgtaat ttgcctggta gacttaacag agcttattcg
     2641 tcgttctatg tcttttaagt tagccatcta ttattcttcc gcgccttctt ctttcttagg
     2701 catatttttc ttaaacttct tatcgaaagc atctaattgt ttgtccaatt cagcttccaa
     2761 cttctcatca aacttctttt cctttttcaa agtatccaaa attttcttgc cgcttgtctc
     2821 taagtatttc attaactcat cacgatatgc aagaacctct tcaatttcaa tatcatcgat
     2881 atgccccttg ttagcagcat aaataacaca tgcttgaagt tcaacaggaa ttggcatata
     2941 ccttccttga accaataatt gagtcatatg agaaccgcgg ttaagctgtt gctgagttgc
     3001 cttatccaag tcagaaccaa attgggtaaa tgcttgcagc tctcgatacg atgccaaatc
     3061 aagacgaaga ttaccagcaa cctgtttcat cgccttaatt tgtgcgttac caccaactcg
     3121 cgataccgaa ataccaacat ctacagcagg gcgttgtcct tggaagaaca aatcagtctg
     3181 caaataaatt tggccatcag taattgaaat aacatttgta ggaatataag ccgaaacgtc
     3241 tcccgcctga gtttcaataa ttggaagtgc ggttaacgat cctgcgccat gttcgtcgga
     3301 gagcttaaca gcacgttcca acaaacgact atgcaaatag aaaatgtcgc caggataagc
     3361 ttcgcgtcca ggaggacgac gcaaagttaa cgacatttga cggtaagcaa cagcctgctt
     3421 tgacaaatca tcataaacgc aaaggacatg cctaccagga tttttagcat tcgccggctt
     3481 accatcggca ccgttataca taaagtattc accaatagca gcacctgcca taggagcaat
     3541 gtattgcaaa ggagctgaat cagatgcatt agcagaaaca ataattgtat gatcaagagc
     3601 accgtgcttt gccaagatat cagcagttct tgcaaccgaa gacgccttct ggccaattgc
     3661 aacataaaca caaatcatgt cttcgtcttt ttgattaatg attgtatcaa ttgcgatagc
     3721 actcttacca gtttggcggt caccaataat taactcacgc tgaccacgac caattggaat
     3781 catcgaatct acagataaaa gaccggtttg aacaggctca tgaactggct ttctatcaat
     3841 aacgccagga gccttaaact caattggacg atgcccagaa attttaattg gtcccttgcc
     3901 gtcaattgga ttgccaagtg ggtcaacaac acgaccaagc atgccgtctc ctactggaac
     3961 ttccaaaaca cgaccggtag ttttaactaa atcgttctcc ttaatttttg taacattagc
     4021 aagaagaaca gcacctacct cttcgtcctc taagttttga accaaaccgt aaacactttc
     4081 accatcagaa gaaacaaatt ccaaaagctc acccatcata gctccggcaa gtccgtaaac
     4141 tcgagcaata ccatcgccaa cctgaataac cgtgccttgt tctttagaca taaccacggt
     4201 tgagatctct tcaatctgag cctttaggtt atttgcaata ctctctgcgg ttaaattttt
     4261 tacatctgct tctttttttg ccataacttc tctccatttc taaaaacgga ttttatccat
     4321 aatgatgcgc cgaagtaagt tcggacttcg ctcgctccat cattgtaagc aagctacaat
     4381 caacaatgcg attcctcgca cttaaaatta cgccacccaa aatatttttg tcaacgtgct
     4441 catttaagac gatgttcgca tcaagttcat tttgagcctt cgttttaata agctcacgca
     4501 aatgatcgtc aagttctaca gcagaaacaa catttacaac aataacatcg aagtgctctt
     4561 ctaagagctt gttgtaagtt tcatacattg acatgcacaa atacaagtct ctgttttctg
     4621 ccataacact caaggtatcc aaaacagctt cgctagctcc tgagaaaatt tctcgtacaa
     4681 gcttgcctga atcaatcttc ttccttgatt ccctttcgta ttcatcgact tgatcacgga
     4741 agacatctgc caattgaaca tttcccattg ttgccttcaa aatattgttc aagtcatcaa
     4801 gaacacgcaa agcttcatca atgccatcaa ttaaaacggc attaagcaaa atgctttctg
     4861 cataagcttg ctgtttcttc cttcttgttg aacgatcatc tttcatacgt taagccttta
     4921 aactcccagc ttctttaaca tatttttcga tcaatttacg atgctctttg tcatcaagat
     4981 cagctgaaac aaacttttta acagccgaca ttgcaatgtc aactgcttga attttaatgt
     5041 cttgctcagc cgcttttttt tgttgattaa ttgccaactt tgccttttcg gtagttgatt
     5101 cggcttgacg ttttgcagcc tcctcaatat tcttaccaat ttcagagctt gaaagtttag
     5161 cttccttcaa aatttcatcg gcttggcttt cagcttcttc gagcatcttt ttattttcct
     5221 ctaagagctt ttctgcctca agcttttttt cttcagaatc ctttagagct ccttcgattt
     5281 tctctttacg agtctccagc attccttcga acttaggcca gccgaacttc catagcacaa
     5341 tagcaacgat gataaaagca atgagcatcg gaataaattc gatcatatta ggtaaaatta
     5401 cctgtatgcc tgaactttcc attacttact cctaaataat caatcttttg cctttagctt
     5461 gcaatcaatg ccaaaacaaa gccgatgagg gccaatgctt cgaccaaagc tgcaccaata
     5521 ataaagttgg taaataatct accttgtaat tctggttgac gagcagttgc catacatgca
     5581 ccataacatg caagaccaat acctaaagca ggtccaataa ctgcaagacc ataaccaatt
     5641 acctttaaac cagctgcaat ctccataata attacctcct cttatttctt acctttttta
     5701 atgttcgctt gtcgcaagtg atatatacac cgcagacaag gttgtaaata cgtaagcttg
     5761 caagaacgca accaagcatt ctaagcaata catcaatata ataaatacca gccaagcaaa
     5821 cgatggaagc accagcaaat actcagcgtt ttgaattgca taagttgcaa atacgctcgt
     5881 cattaaagcg aagatcccca aaaccatgtg accagcaaac atatttccat aaagtcgaaa
     5941 tgccagtgtc atagcacgca agcacaatga aagcatttcg aagaaccaaa taaccggaac
     6001 cataacaatt ggcaaaccac taggcgcgat acttttgatg tagcctcaac caccttttgc
     6061 ttttacaccc caataattaa agtacacaaa agagataata gccaaggccc atgtaatact
     6121 tattgatcca gttgctgctt tagctcctgg aataaggcca acaaagttag caacaaggat
     6181 aaagaaaaac agcgttgcta aaaatggaac atgtctttta taaccatgac caacgctatt
     6241 cttgcccata tcatttcgaa caaattcata gccatattca accatgttca aaaacttgtt
     6301 tttaggaact aaagaaagct tacgaccaac aaccaaaata gcaacaacaa caattatcat
     6361 tacaataatc atccaaaaga tgtagtttgt aataccgaag ttttcgttac caaaaactat
     6421 cgaaggattc atcaaatcat tctgaagctc ggttaagtgt tctggcaatt cctgtagtgg
     6481 acccattatt tacctttact cttcttggac ctattggccc tttcctttgc tgctttgcta
     6541 cgtttaatta cagtgtaaat tccgtaaaca atcgcaaaca aaatcaacgt tattgctgca
     6601 gctaatgcga aggctaaaag cccatctttt gctacaaaat agcaaattaa aactgcaaca
     6661 actaaaataa ttagcgaagc aaaaaccccc aggataagta gtgccccata accaaaattg
     6721 gaagttttag ttacgttttt tgaagctttt atacttaaaa gcaatggtac aaacgataaa
     6781 acaccaacaa cagcacctag aaatacaaac atttaaatga attctcctat tagttttacc
     6841 cgctaaacac attggtaatt atacacaaac ttacccacca aggacttaaa attttgtaaa
     6901 gcattggctt gggtatattg tgcaagagtt ttagagctaa taacaaaatc ctcttcgctt
     6961 ttaaatttgt tttgtgattc agttcaatcc ttttgcatca aaagcaagca aaatgtccta
     7021 aacgcaggcg atgcccaaga agcaaagtca atcgcaagat ctttatgcaa atatatttct
     7081 ggggaatcaa aagttttgat attgattccg caaacattgc acaaagaaat taactttaga
     7141 agatctggcg ccgcacctac tcctaaagct tcgctacaca acttaatata ttcgtcctta
     7201 ttaaaatcaa tgttgtgtag agtttctcat aattggataa attcaagagt tgttggttca
     7261 ctaagccact tttttaatgc tcaagttgga tcgatatcgt acccataaca aattttacta
     7321 aacgacataa acactttttc attttttggt gatttcgcta cattattcat gagtcctcct
     7381 ttcaacaaaa acttagagga tcttgcattg ttatgccagc tcatgaatga caaatggacg
     7441 cttccgcgca atgcattggc tctttaagtg cattctacac caaaatagaa caaatgtttc
     7501 ataaaattat aactttttaa aattaattat ttgataatca cgaagcccac tacaagcaag
     7561 caattcgcga acaaaaattt caggattcag cgacccatca ctaaaatttt tcaaagaaaa
     7621 actcaattca gtgcatcctt tggcgctctt atgttcaact tcgccgtcta aaaattttga
     7681 cacatcaaat tctttttctt tgcgcttttt aacaacctta attttatcgg gcactttaat
     7741 atctttaatc tcgcaagcta cattcacact aaatttacta acttcctcaa tttcaaaagc
     7801 ttgcttgtta tcaacataag cacaacttag cggttcaagg caactttggc ttgcgtcttt
     7861 caaagcagcc aggcacttct ccggagcaat atagtcagtc aacaaaatat caaaaacgtt
     7921 atcttcgctt tcaatgccaa cacttagagc ttgcgcaaac gaaattctca tatgtggcga
     7981 aaatccttcg ctaactgcat atggaagtcc acttcggcga attgcatgtt ctaaagcgcg
     8041 agctaattca agttgcgaag tccaggccgc aagtccaacc ttcttgaaat gaactctcaa
     8101 cttaaacttt ctattttgca ttgcaccctg cattatcgct tccccgcaat ttcattttta
     8161 actttaaggt tttgacaaat gccacaattt tgacacttat caaaagtgca atctggagtt
     8221 gtttttgcgg cgtttgcttt ttcgcgttcg ctttcaaaga attttctatc aacaccaaaa
     8281 tcgatatgag accaaggcaa attgccatct aaatcctgcg aagccaaaac ttgtaagttc
     8341 atccctaact tatcagcagc agaaatccat gcatcaaaat taaaaaattc atcccaggca
     8401 tcaaacttcg caccgcgttt ccaagcctcg taaattaagt tacaaacatc acggctacct
     8461 cggcttataa cagcctcaag catacttgtt tttggatcgt gtcaagaaaa actaatcgcg
     8521 cgacttttga tatttttgcg aattaaatta atacggcgca tagcctcgtc catagaaatt
     8581 tgcccataaa cctcaaacgg agtttgtgcc ttcggaacaa aaatc
//
LOCUS       DBNCDC010000042         8623 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_3797_length_8623_cov_7.629902, whole genome shotgun sequence.
ACCESSION   DBNCDC010000042 DBNCDC010000000
VERSION     DBNCDC010000042.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 8623)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8623)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8623
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3797_length_8623_cov_7.629902"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            157..219
                     /locus_tag="AC04VQ_04185"
                     /pseudo
     CDS             157..219
                     /locus_tag="AC04VQ_04185"
                     /inference="COORDINATES: protein motif:HMM:NF015922.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            223..579
                     /locus_tag="AC04VQ_04190"
     CDS             223..579
                     /locus_tag="AC04VQ_04190"
                     /inference="COORDINATES: protein motif:HMM:NF015922.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HIO9553366.1"
                     /translation="MNVAQEGAELLVITENGYGKRTPLTEYPDQNRGGKGVFTIKMTE
                     KKGRLAVMKVVKPEDEVMLISQNGILVRTQIAGISQLGRATQGVRIMNVGQSDKVCAV
                     SKIASEEGEEEPETLV"
     gene            585..773
                     /locus_tag="AC04VQ_04195"
     CDS             585..773
                     /locus_tag="AC04VQ_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553367.1"
                     /translation="MAKDFKQFIDEKQGSLRASLANVWSVFKRDLKRIFTRPLLILVF
                     IGALLLPCLYAWLTIYAN"
     gene            918..1856
                     /locus_tag="AC04VQ_04200"
     CDS             918..1856
                     /locus_tag="AC04VQ_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HIO9553368.1"
                     /translation="MDKDSAFEKLEHGEVYAIIVLPQDFSNSIAKIFIDSNNRDFARA
                     QYFVNERENGAAVKITDTGASTIEKQINEKFVGKVTEVVAGKVVELSGALVVDVANSG
                     NTMTDRINRVAANVSGLNNVIKKGQGTISQAQQTISSAKSVLTDVKNESDNLQNIVNN
                     AKQKAQDLKDSLLQYIIEAELPYSTIAVIINMIDDLSSSLSDLSSSLFIVSHSCDEII
                     SQLHEASYLLDGTNSIAADFSAKMSKVHDLLLEVSTTIQANLTNAPDVVKALTNANTQ
                     TVGAFMSQPVKLETEVLNPIANNGTGISPFFTNLAL"
     gene            1953..2291
                     /locus_tag="AC04VQ_04205"
     CDS             1953..2291
                     /locus_tag="AC04VQ_04205"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HIO9553369.1"
                     /translation="MLFSVGAIISSLFVSLGNLVIGIQCENPVVYVLTCVIIAIVFVN
                     IIYTLAACFKHVGRAIACILVIMQIPGSSGMFPIQLMPQVFQNIFPFLPFTYGINALR
                     ECIISIDWLY"
     gene            2337..2801
                     /locus_tag="AC04VQ_04210"
     CDS             2337..2801
                     /locus_tag="AC04VQ_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553370.1"
                     /translation="MIGIYARNGLRGINRLFDEELYKNTIMVCEEQDHLKAKASTVET
                     VVESLSDQEEALTMTDAIDAIRKKYKKKSRYGLIVLATAPTLLLIIMSIYMSFVDVDI
                     NTKLIWMFAWMLSIIIIATYVIVNEYKYNQFKKQELNSLMKASDLKAGNANA"
     gene            2938..4008
                     /locus_tag="AC04VQ_04215"
                     /pseudo
     CDS             2938..4008
                     /locus_tag="AC04VQ_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03061.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
     gene            4216..4599
                     /locus_tag="AC04VQ_04220"
     CDS             4216..4599
                     /locus_tag="AC04VQ_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HIO9553371.1"
                     /translation="MLTMWVVSICFSLFIYTMLASFGSIGKALCVILLVLQICATGGT
                     FPIQLVMEPFQAISPFLPGTYALKAINMCVAGFSGVDLYMCLGELILTIVPLSLLLGM
                     VLRNPIIKVNEKFKEKVSEANLLAI"
     gene            4610..4942
                     /locus_tag="AC04VQ_04225"
     CDS             4610..4942
                     /locus_tag="AC04VQ_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009307148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIO9553372.1"
                     /translation="MSNEDCIFCKIVAGEIPSTKIYEDEKCYAFEDLEPIAPVHILLI
                     PKEHYDNIGDNVSTELLGYLLTKAHEIAEMKGIKDSGYRVITNTGNDGGQSVKHLHLH
                     IVGGKQLR"
     gene            complement(4948..5928)
                     /locus_tag="AC04VQ_04230"
     CDS             complement(4948..5928)
                     /locus_tag="AC04VQ_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553373.1"
                     /translation="MAAFIIAAILGNFIHTGLNSFDAKVSQGEGGRVKVEASVTDLRS
                     KNIDYGDSVKFNFSSYYVAEDVPILNGEFLNTGQHIATAADEASPLIFYIQGSSNFWT
                     IANLDDSATVKITLTDKGKYKNLFNAFNTPISGDVKNLRSLRGGNLKLLDIFRGANPK
                     TNYDVESTLYYYYQNISIQNRINLDENTYNVHSIDIKNKDCNTMIIDRLRQICQLSGR
                     TYIYSNTPDMTAYFCAIIEAIGGASYDEIVNDYMQTYANIFGITRETNPEEYEAIKTY
                     HIDWFLHKFTKTPSNYDMHGCDFAYDAEMYLRANGASSNDINLIRTRLTA"
     gene            complement(5984..6991)
                     /gene="pta"
                     /locus_tag="AC04VQ_04235"
     CDS             complement(5984..6991)
                     /gene="pta"
                     /locus_tag="AC04VQ_04235"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HIO9553374.1"
                     /translation="MKGDKMSAFLDKIIDKAKSDKKTIVMAEGNDPRTLDAAEKVLER
                     GIANIVIVGNEDEIKGSGKKLDGATIMDPKNSELHDEFANELYELRKEKGMTPEKASA
                     AVEDDLFFGTMLIKTGHADGMVAGACHATSDVLRPALQILKTAPGVKYVSSYFVMIVP
                     NCEYGEDGAFVFSDCGLEPMPDVDKLANIAVNSAKSFENVIGAEPRVAMLSHSTKGSA
                     NGNDDQIKMEEATEAAKALAPDVKIDGTLQLDAAIIPEVAASKAPDSDVAGKANVLIF
                     PDLDAGNIGYKLVQRLAKAEAYGPVTQGIAAPVNDLSRGCSADDIVGVVAITAVQAQF
                     V"
     gene            7122..8369
                     /gene="glf"
                     /locus_tag="AC04VQ_04240"
     CDS             7122..8369
                     /gene="glf"
                     /locus_tag="AC04VQ_04240"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009307145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HIO9553375.1"
                     /translation="MKFDRIAHAMSGVNYKKFHDVEVSEFDAVVVGCGFAGAVCASEL
                     ARNGKKRVLIIEKRDHIGGNMFDEYDKAGILVHKYGPHIFHTSSKLAADYIKKYTKTI
                     PYEHRVKADIHGTYMPVPFNKTSMEIAFGPERANELTHKLIKKFGNEVKVTINELRQE
                     EDKDLSEIADFVYENVFLHYTEKQWGLTPEEVDPSVTARVPIFISRDDRYFQDRYQFM
                     PKEGYTKLFENMLDHKNITVALNTEAESVFGLEFEGNAEDDKLTGITIQYKKYDKPII
                     FTGPLDELFLGRFGRLPYRSLDFKFETLDKEYEFPCGTINYTVSEDFTRITEYKHLTG
                     QKCDKTTIMKEYSRAYEDPKTQIPYYAIINDENNAHYEKYKNLVDSMPNFYAIGRLAE
                     YKYYNMDQIIEKAILLAQEILDK"
     gene            8370..8549
                     /locus_tag="AC04VQ_04245"
     CDS             8370..8549
                     /locus_tag="AC04VQ_04245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIO9553376.1"
                     /translation="MNKPLNKVITFAIPCYNSAEYMDNCIEHLLKLNSKDDDIEILIV
                     DDGSAKDNTLEIAKS"
BASE COUNT         2700 a         1440 c         1650 g         2833 t
ORIGIN      
        1 gcgctttatg atacaaacag agctaatggt ttgattgcga ttaagctcaa agaacatgat
       61 cgtttagttg acgttcaacg tgttaagaaa aacgatcgcg taattatggt atcttccgaa
      121 ggcaagtcta ttatgtgaga cgaagagcag gctcgcccga ctggtcgtgg atcgatgggt
      181 gttaagggca tgaatttgcc aggcgatgcc aaacttttag gcatgaacgt tgctcaagaa
      241 ggcgcagaac ttctcgtcat aactgaaaat ggctatggaa aacgtactcc gctaactgaa
      301 tatcctgatc aaaaccgtgg aggtaaaggc gtcttcacta ttaagatgac cgaaaagaag
      361 ggccgtcttg cagttatgaa agttgtaaaa cccgaagatg aagttatgtt gatttcgcaa
      421 aatggaattt tggttcgcac tcaaattgct ggaatttcgc aacttggtcg agcaactcaa
      481 ggtgttcgca ttatgaacgt tggccaatca gataaggttt gtgcagtttc aaaaattgca
      541 agtgaagaag gcgaagaaga gccagaaact ttagtttagg ctaaatggca aaagatttta
      601 aacaatttat tgatgaaaag cagggaagcc ttcgagcttc ccttgctaat gtttggtctg
      661 tttttaaacg tgatcttaaa agaatattta ctcgtccatt attaattctt gtttttattg
      721 gagctctttt acttccatgc ctatacgcgt ggttaacaat ttatgcgaat tgagaccctt
      781 atggcaacac tggaaacctg aaagttgcac ttgttaatga agatttaggt gttgatagtt
      841 ctgttggtgg acatttggca attggcgatg agcttgagaa tcaattgtct tcaaataatc
      901 aacttggatg aacttttatg gacaaagaca gcgcttttga aaagctcgaa catggcgaag
      961 tgtatgcgat aatcgtactt cctcaagact ttagtaattc gattgctaaa atatttattg
     1021 attcaaataa tagagatttt gctagggcgc aatattttgt taatgaacgc gagaatggtg
     1081 ctgcagtaaa gattactgat actggggctt cgacgattga aaaacaaatt aatgaaaagt
     1141 ttgttggaaa agtcaccgaa gtagttgcag gcaaggttgt tgaactgagc ggtgctttag
     1201 ttgttgatgt tgctaatagc ggaaatacaa tgactgaccg aattaaccga gttgctgcga
     1261 acgtctctgg tttaaacaat gttataaaaa agggccaggg aacaatttct caagcgcaac
     1321 aaaccatttc ttcggcaaaa agtgttttaa ccgacgtcaa aaatgaaagc gataacctac
     1381 aaaatattgt taataatgcg aagcaaaaag ctcaggatct aaaagatagt ctacttcaat
     1441 atattattga agcagagctt ccatattcca ccatagctgt aattattaat atgattgatg
     1501 atttgagttc atctttaagc gatctttcat cttcgttatt tattgttagt cactcttgcg
     1561 atgagataat tagtcaatta catgaagcct cgtatttgct tgatggtact aattctatag
     1621 cagctgattt ttctgctaag atgtcaaaag ttcatgattt gctcttggaa gtctcaacaa
     1681 ctatacaagc taacttgact aacgctccag atgttgtaaa agcgttaact aatgcaaata
     1741 ctcaaactgt gggtgctttc atgagtcagc cagttaagct tgaaactgag gttttaaatc
     1801 ccattgctaa taacggaacg gggatatctc cattttttac taaccttgct ctttgagtat
     1861 tgggaattgt tctaattgct ttatttagaa ccgaagttga gcccccaaag cgtaaaggtt
     1921 gaaatgcgaa gcaagcatat gttgccagat ctatgttgtt tagcgttggc gcaataattt
     1981 caagtttgtt tgtttcattg ggcaacttag ttattggtat tcaatgtgaa aatcctgtag
     2041 tgtatgtttt aacttgcgta attattgcta ttgttttcgt taatattatt tatactctag
     2101 ctgcttgctt taagcatgtt ggacgtgcta ttgcatgcat tttggtcatt atgcaaattc
     2161 ctggaagttc gggcatgttc ccaattcaac ttatgccaca agtatttcaa aacatatttc
     2221 cgtttttacc atttacttat ggcatcaacg cgcttcgtga atgtatcata agtattgatt
     2281 ggctttattg aggtttagac gtttttgcat tgcttgtttt ctttgccatc ttttttgtga
     2341 ttggaattta tgcaaggaat ggacttcgag gaattaatcg actttttgat gaagagcttt
     2401 ataagaatac aatcatggtt tgcgaagaac aagatcatct taaagcaaaa gcttcaacgg
     2461 tagaaacagt tgtcgaatcc ctgtcagatc aagaagaagc cctaacgatg acagatgcta
     2521 tagacgctat tcgaaaaaaa tacaagaaaa aatcaagata tggccttatt gttttagcta
     2581 ctgcgccaac attgcttctt attattatgt ccatatatat gagttttgtc gatgttgata
     2641 ttaatacgaa gttaatttgg atgtttgctt ggatgctctc aattattatt attgctacgt
     2701 atgtaattgt taacgaatat aaatacaatc aatttaaaaa acaagagtta aattctttga
     2761 tgaaagcttc agacttgaaa gcaggtaatg ctaatgcgta atgtttgaaa aataatcaaa
     2821 gatgatttta aacttttgtt ttcaaatgtt attagcattg tggttgttat tggattaatt
     2881 gtagttcctg gaatgtatgt atgatttaat ttggcaggag tttgagatcc ttataataat
     2941 gttgacaact tagaagttgc tattgttaat caggatgaag gttataagag cgaagttttg
     3001 ccaatggata taaaagttgg taatacttta gtaacatctc ttcatgagaa taaaggtttt
     3061 aaatgggctt ttacagattt cgatgatgct atgaccaaat taaaagatgg aactttttat
     3121 gctgtaatag ttatccctaa ggattttagt acacagttac tttcaattat tggtggcgaa
     3181 gtcgagtctg caaacttgga atattattcc aacgaaaaaa gtaatccaat tgcaccaaaa
     3241 attacgggga agggcgcttc gacaattcaa caaaaaataa ataatgaatt ttcgacggct
     3301 atatattcaa tcattttaaa aactgcttcg aatttattaa actcaagtac tgttgatgat
     3361 gcatctagct taggaaaaac tttgataggt gttttatctg gcgcgcgcga ctcaatggac
     3421 aacgttattt ctgaaatatc tttaattaag cttgaactaa atacgctgaa agaaactgtt
     3481 gatcaaattc aaaaagaact tccaagttct ggttcgactt tgtttgaaaa tattaaaaat
     3541 tatttagaca attctaagca agaactatta agtagtaagg aattgattga gcaattaaaa
     3601 tttattattc cggatcaact ttacaatgat tacattactt taataaataa tcttttgtct
     3661 tctatagaca attgttataa cattgttaac aatgcagcaa gaacctcgga tgatttatcg
     3721 gctaccttgg tttcgttaag tgaagttctt aatgatctta atgagcaatt atcctcaatg
     3781 caagatacct tcacattgat ttctggtgat tttaattctg caagagagag gttaagcctt
     3841 ataacaactt ccacatctgt tgatgattta agaaaaatta ttggtgaaga ttcaaataag
     3901 tttgcaactc taattactaa gccagtttta atggagagaa atgttgtttt cccaatggta
     3961 aataatgcgg cttcgatgag tggattttat attgcaattt gtatttgagt tgggtcctta
     4021 cttttggttg cacttcttaa atctgaatta acaaaaaggc gtgcaaaaga atttaagaaa
     4081 caaaatctta aaaattgaca aatatattta ggcagatatg ttatttttgg agtaatctca
     4141 ctttgccaag ttacctttat tgcactgggt tgtctattct tcttgcaaat tccttgcgca
     4201 catccagttt tatatttgct tactatgtgg gtagtttcta tttgctttag tttgtttatt
     4261 tatacaatgc ttgcttcttt tggaagtatt ggaaaagcgt tatgtgtaat tttgcttgta
     4321 cttcaaattt gcgctactgg cggaacattc ccaattcaat tagttatgga accttttcaa
     4381 gcaatttctc cattcttgcc tggtacttat gcgctaaagg caattaatat gtgtgtagct
     4441 ggcttcagtg gagttgatct gtatatgtgt ttgggcgaat taattctaac aattgttccg
     4501 ctatcattat tgttgggaat ggttcttcgc aaccctataa ttaaagtcaa cgaaaaattt
     4561 aaagaaaaag tttctgaagc aaatctatta gcaatttaag gaggataaaa tgagtaatga
     4621 agattgtatt ttttgcaaga ttgtagcggg tgagattccg agtacaaaaa tttatgaaga
     4681 tgaaaaatgt tatgcttttg aagatttaga acctattgct cctgtgcata ttttgcttat
     4741 tccaaaagag cattatgaca atattggaga caatgtttct acagaattgc taggctattt
     4801 attaacaaag gcacatgaga ttgctgagat gaagggcatt aaagattctg gctatagagt
     4861 tattaccaac acaggaaacg atggcggaca aagcgtcaaa catcttcatt tacatatagt
     4921 tggtggaaaa caacttcgtt gagaagctta agcggttaaa cgagttctta ttaaattaat
     4981 atcattgctt gaagcgccat ttgcgcgaag atacatttct gcgtcatagg caaaatcgca
     5041 tccgtgcata tcataattgc ttggagtttt tgtaaacttg tgcaagaacc aatcaatatg
     5101 ataagttttt atagcttcgt attcttctgg atttgtttcg cgagtaatac caaaaatgtt
     5161 tgcatatgtt tgcatgtaat cgttaacaat ttcatcatag cttgcacctc caattgcctc
     5221 aataattgcg caaaaatatg cagtcatatc aggtgtgttg ctataaatat atgtcctgcc
     5281 gcttagctga caaatttgac gtagtcgatc aattatcatg gtgttgcaat ctttgttttt
     5341 gatatcaatt gaatgaacgt tataagtatt ttcatccaaa ttaattctgt tttgaattga
     5401 gatattttga tagtaataat atagagtgct ttcaacatca taattagttt taggattcgc
     5461 gcctctaaat atatcaagaa gttttaggtt tcctccgcgt agagatctta aatttttgac
     5521 atctccacta attggcgtat taaaagcatt gaataaattt ttatatttcc ctttatcagt
     5581 aagtgtaatt ttcaccgttg ctgaatcatc aaggtttgca attgtccaaa agttagatga
     5641 cccttgaata taaaaaatta ggggcgaagc ttcgtcggct gccgttgcaa tatgctgacc
     5701 tgtgttcaaa aattcgccat ttaggattgg aacatcttcg gcaacataat aacttgaaaa
     5761 attaaactta acagaatctc cataatcgat gtttttgctc cgaagatctg taacgctggc
     5821 ttcaacttta acacggccgc cttcgccctg gcttactttt gcatcaaatg aattaagacc
     5881 cgtatgaata aaatttccaa gaattgcagc aattataaag gctgccaaaa ttccgacagc
     5941 caatataatt cgtttgttat ttgtccaaaa ctttttcaat tacttataca aattgagctt
     6001 gaacagctgt aattgctaca acgcctacga tgtcatcggc actgcacccg cgagataaat
     6061 cattaactgg tgcagcaata ccttgagtta caggaccata agcctctgct tttgcaaggc
     6121 gctgaacaag tttatatcca atgtttccag catctaagtc tgggaaaatt aaaacgtttg
     6181 ctttgccagc aacatcacta tcaggtgcct tagaagcagc aacttctgga ataattgcag
     6241 cgtctagttg caaagttccg tcaatcttaa catcaggagc aagtgccttc gcagcttcgg
     6301 tggcttcttc cattttaatt tgatcatcgt tgccgtttgc agaacccttt gttgaatgtg
     6361 aaagcatagc aacacgaggc tcagcaccaa taacgttctc aaaagatttc gcagaattaa
     6421 ctgcaatatt tgcaagcttg tctacatctg gcattggctc aagaccgcaa tcgctaaata
     6481 caaaagcgcc atcttcgcca tattcacaat ttggaacaat cataacgaag taactgctta
     6541 catatttaac tccaggtgca gtctttaaaa tttgaagagc cggacgcaaa acatcgcttg
     6601 ttgcgtggca tgcacctgca accataccat cggcatgacc tgtttttata agcatagtgc
     6661 cgaagaatag gtcatcttca acagcagcac ttgctttttc tggagtcatt cctttttctt
     6721 tacgaagttc ataaagctcg tttgcaaatt catcatgaag ttcagaattt ttaggatcca
     6781 taattgtggc accatctaac tttttgccac ttcctttgat ctcatcttca tttccaacaa
     6841 taacaatgtt agcaataccg cgttccaaaa ctttttcagc ggcatcaaga gtgcgagggt
     6901 cattaccttc agccataaca attgttttct tgtcagattt tgctttatca atgattttat
     6961 ctaaaaatgc agacatctta tctcctttca taattcctgc tatttaatca aatctcaacg
     7021 attcgctatt atacattttc ttggggccgg gggcagtggg atttcattat tttttatttt
     7081 tatttatttt tagttgaata aaatccactg cgccattagt tgtgaaattt gatagaatag
     7141 cccacgcaat gagtggagtt aattacaaaa aatttcatga tgtagaagtc agcgaatttg
     7201 atgctgttgt tgttggatgc ggtttcgcgg gtgcagtttg cgcaagcgaa cttgctcgca
     7261 atggtaaaaa gcgcgtctta attattgaaa agcgcgacca cattggtgga aatatgtttg
     7321 atgaatatga taaggctggc atattagtcc ataagtatgg tccacacatt tttcacactt
     7381 caagcaaact tgcggctgac tatattaaaa aatacacaaa aacaattcct tacgaacacc
     7441 gcgttaaggc tgatattcat ggcacttata tgcctgttcc tttcaataaa acttctatgg
     7501 aaattgcttt tgggccagag cgcgccaatg aacttacaca taagttaatt aagaaatttg
     7561 gaaacgaagt taaagttacc attaatgaac ttcgtcaaga agaggataag gatctttccg
     7621 aaatcgctga ttttgtttat gaaaatgttt tcttgcacta cactgaaaag cagtggggac
     7681 tgactcccga agaggttgat ccttctgtta ctgctcgtgt gcctattttt atttctcgtg
     7741 atgacagata ttttcaagac aggtatcaat ttatgccgaa ggaaggatat acaaagctct
     7801 tcgaaaatat gctggaccat aaaaatatta ccgttgctct taacaccgag gcagaaagtg
     7861 tcttcgggct tgaatttgaa ggaaatgccg aagatgacaa gttaactggc attacaattc
     7921 aatataaaaa gtatgataaa ccaataattt ttacaggtcc gcttgatgag cttttcttgg
     7981 ggcgcttcgg tcgtcttccg tatagatcgc ttgactttaa gtttgaaacg cttgataagg
     8041 aatacgaatt tccttgtgga acaattaatt atactgtgtc tgaagacttc actcgcatta
     8101 ctgaatacaa gcatttaaca gggcagaagt gtgataaaac aacaattatg aaggaatata
     8161 gtcgagccta cgaagatcct aaaactcaaa tcccatatta cgcaatcatt aacgatgaga
     8221 ataatgcgca ttatgagaaa tataagaact tggttgattc catgcctaac ttttatgcaa
     8281 ttggtcgttt ggccgaatat aaatattaca acatggatca aatcatagaa aaagcaattc
     8341 tattggcgca agaaattttg gataaataga tgaacaagcc tttaaataaa gttattactt
     8401 ttgcaattcc atgctacaac tctgctgaat atatggataa ttgcatcgag catcttctta
     8461 aattaaattc aaaagatgac gatattgaaa ttttaatagt cgatgatggc tcggctaagg
     8521 ataatactct tgaaattgca aagtcttgag agaaaaagca tccaaaagtg attcgtgcca
     8581 ttcatcaaga aaatggtgga cacggacaag ctgtaaatac tgg
//
LOCUS       DBNCDC010000043         8602 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_3816_length_8602_cov_7.095121, whole genome shotgun sequence.
ACCESSION   DBNCDC010000043 DBNCDC010000000
VERSION     DBNCDC010000043.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 8602)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8602)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8602
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3816_length_8602_cov_7.095121"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(1..1021)
                     /locus_tag="AC04VQ_04250"
                     /pseudo
     CDS             complement(1..1021)
                     /locus_tag="AC04VQ_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
     gene            complement(1190..2140)
                     /locus_tag="AC04VQ_04255"
     CDS             complement(1190..2140)
                     /locus_tag="AC04VQ_04255"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIO9553377.1"
                     /translation="MPKSKIDQDIRYGCRTDIGQVREHNEDSLLAESPLFVVADGMGG
                     HAAGEVASEIAIESMRKNAPREADCEGLAKAVEGANQEIIKAALEGKGKEGMGTTMTA
                     AVIEGNRLCIAQVGDSRAYLLHNGRLQQITRDHSLMADLIEQGQITPAEARVHPNRSL
                     ITRALGNDPEMFADQYEIDIEEGDRLLLCSDGLCGMLTDDIIEGWMNKVQDPQECANK
                     LIEQANSFGGLDNITVIVADLNSFSAKKKKGIKKSRRNAIIVVVAAIAILVGGVVGAG
                     AIISNSAYLCEEEGYVSIYKGLPGTFAGFDLSQKEYGSDV"
     gene            complement(2133..2546)
                     /locus_tag="AC04VQ_04260"
     CDS             complement(2133..2546)
                     /locus_tag="AC04VQ_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIO9553378.1"
                     /translation="MIDIILLICKLALIALLYLFLFAIMKTGIGLVKGQRKADKTWNI
                     AVERGPKELRGVSIQVNGPVIVGRSAGCDIVIGAGYVSGRHARFSLMGNDLFIEDLGS
                     TNGTAVNGKIIEGPYNLRDKDTVSVGDVDIRVRYA"
     gene            complement(2562..3659)
                     /locus_tag="AC04VQ_04265"
     CDS             complement(2562..3659)
                     /locus_tag="AC04VQ_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009608449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HIO9553379.1"
                     /translation="MGIFSKFENKMEDAIDDLGDKFFDSPISPVQIAKKAEKQMRRNK
                     MLGAGKQFAPTLYTVLVNANDDARLFGYYPTLAGETETYLKAKANESGLAMDGDPLVR
                     FIVDNDLKAGKFDVIAEPVAADIITQLRAEEMERYGINNQNQPVVSQTPQQAAAEVAA
                     QAQVNPTPQVSTPQNVSSPFAPFEEVPAQGVASPAPAVQNTPVPQPTPAPQPTPVPQA
                     TPAPVEVPVQEPNQAEAVGGNANPVPVSSAKTAVFAGNFDTPNANAAPQQNAAIPPAH
                     LMEQTTGRVYTLNAATVVIGRENTCNIVLPDANASRQHAQITYNQGLWLCNDLGSTNG
                     TFVNNQEITTREIHPGDVITIGVTKLTFNVG"
     gene            3893..4036
                     /locus_tag="AC04VQ_04270"
     CDS             3893..4036
                     /locus_tag="AC04VQ_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553380.1"
                     /translation="MKKREVLMILVSCVLICGQVYFDLKIPDFIDEMTILINTAGASA
                     NRT"
     gene            4052..4735
                     /locus_tag="AC04VQ_04275"
                     /pseudo
     CDS             4052..4735
                     /locus_tag="AC04VQ_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            4931..5653
                     /locus_tag="AC04VQ_04280"
     CDS             4931..5653
                     /locus_tag="AC04VQ_04280"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIO9553381.1"
                     /translation="MLKRISFSIKKGETVAIIGSTGSGKTTLVNLMMRFYDATTGHVL
                     LNGQDIKDYSFDTLYNTICMATQKPVIFYGSIDDNIKFGEHNKELTDKEIEEAIEIAQ
                     AQEFIAKLKDKQKTIVSQGGKDISGGQKQRLSIARAVARKPEMLILDDSFSALDYKTD
                     ASLRRILKEKMAGTTIVMVAQRIGTIKNADKILVLENGELAGIGTHDELLESCGTYQE
                     IAISQLRKEELAKITKLKKNGK"
     gene            5643..7529
                     /locus_tag="AC04VQ_04285"
     CDS             5643..7529
                     /locus_tag="AC04VQ_04285"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIO9553382.1"
                     /translation="MANKKKEDTSFDYAQKKDNFQGNKGMKGAPTDGGVNMVSSKDKQ
                     SFKDIISTLGKVFKYVGRHKKVLYAGFILAAASSILLMLGPNLIGQMADAIQNSLDKE
                     IDILTVSALGFTLIVIYGLSNAFSFIQQYIMAGMTAKVCMRLRSDFIQKLNKLPLSYF
                     NTHLQGDILSCITNDIQTLRQGISRCLPGLIKALAQFFTCLIMMIITEWHLTLCVYGV
                     VLLGLIAIMTIMKMSQKFFDQRQSNLGALNGLIEEMYSGYQVIKMSLAKKKVTEQFDE
                     GNEKLYKTDYMSQFISGIMAPMMVIIGNVAILVVIVVGANLAISNVITFGAVAAFLLF
                     CNYCTQPLTRITQYLTDLQGVCAAAVRLFAFLEAEEMPNELLKETEITEPKGEVEFDH
                     VKFSYPSNPDKIIINDFSAKVKPGQKVAIVGQTGAGKTTLVNLLMRFYDVNSGEIKID
                     GIPINDLRRDNIHDMFGMVLQETWLFEGTVRENLVYNLEKVPEERIIEVCKTCGVYDF
                     IVTMPEGFDTKMSESLAISAGQKQLITIARAMLQNAPMLILDEATSSVDTRTELDIQL
                     AMDMLTRGRTSFVIAHRLSTIQNANLILVMEDGDVVESGTHDELIELGGKYFELYNSQ
                     FQES"
     gene            7529..8560
                     /gene="tilS"
                     /locus_tag="AC04VQ_04290"
     CDS             7529..8560
                     /gene="tilS"
                     /locus_tag="AC04VQ_04290"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIO9553383.1"
                     /translation="MAAGPKNTLEVVEATIKKHHLFKKTDYLILMVSGGSDSVALSYI
                     ISELFPKKFAIMHLNHNLRIEANEDAQFVESLAKHLGAPYFGFSENVSGVASQANENI
                     EACGRELRYKYANIVASKFSGNVKICTAHTADDRIENFYMRSIIGTGPGGFASINYTT
                     NNVIRPLLDLPKEALMDFIKSYQNAFKDSSGNLWREDKTNEDTDRFRAYVRHEIIPLA
                     KRQNPQILTNLTNSMNLIADESDYIEEKVSELTIEHFQFSDNSFLIRPGFKNENIALK
                     RRAIYNALLKVFPKGTRLETKSIASILEASEKSNYTDNIQENYSVHSNKNGVLVQPME
                     EYRKSRNRI"
BASE COUNT         2689 a         1744 c         1694 g         2475 t
ORIGIN      
        1 acaaaaagag aagcaaaacg gccgctccgc ctaaaagtcc aagctcttca caaattgcaa
       61 cgaagataaa atcgctctct acaacaggaa tttgagtgca caaaccttga ccaattcctg
      121 ttccaaaaag acctccatcg gccagcgaat atagcgattg aaccagctga taaccagttc
      181 cagttgggtc ttggaaagga tcaagccaaa tactaacgcg gtcttgaaca tgtccgaaaa
      241 aagaatagag aataaatccc gcaacaacgg ccaagccacc agcagctgca aggtagaact
      301 tcttaccgct cgcaacatat aacatagtta taaagaccga gaagaaaaca agagcgcttc
      361 ccaaatcctt ttcaaaaatt acaattaaaa tagaaattgc tcacataatg agcaatggca
      421 taattgttcg aaggtcaggc aacttaaaaa tgccaatttg atgagtgaaa actgaaagca
      481 tttctcggtt atgagctaaa taacccgcca agaaaagagc gatagcaatt tttgcaagct
      541 cgccaggttg aaatgaaaat ggaccaatgc caatccaaat tcgcgaacca taaatttctt
      601 gaccaatgaa cggaatcaat ggagagagca acaaaataaa accagcaatc gccaaggtgt
      661 atttataatt tgcaactttt gcaacagcct ttactaaaat caaagtcaaa atcatgcaaa
      721 ctacaccagc aaaaagccac attacctggc gaagcgccag atcttcgttg atgcgagtaa
      781 taaatgtaat tccaataccc gtaagaacta aaactatagg caaaattaca gggtcggccg
      841 aaggggctaa ttttctaacg gcaaaatgtg caattaaaaa cgaaacaatt attccaattg
      901 gaattaaaac attgccaagg gttaagtcaa gaccacttat aacaacaagc atgccataca
      961 ttatcataac aatgactgct gcaatgccaa gcaataaaag ttctgtcgac ctacgtgtca
     1021 tttactactt cttccccgaa ttcaaacttt gctttttagc gttttgctct ttttgctctt
     1081 gcgcttcttg cttgtaatta tcaagcgcac gccaagcagc atctaaatta tcaacgcgaa
     1141 tcacaccgtc cttaaaatgt tcttgagtgc tttcgcgaag gtctttaact caaacatcgc
     1201 ttccatattc tttttgcgaa aggtcgaagc cggcgaaagt tcctggcaga cccttataaa
     1261 ttgagacata gccttcctct tcgcaaaggt atgccgaatt actaatgata gcccctgcgc
     1321 caacaactcc accaacaagg atggcgatag cggccacaac aacaattatt gcgtttcggc
     1381 gcgacttctt aattcccttc ttcttttttg cactaaagga attaaggtcg gccacaatta
     1441 cagtgatgtt gtccaaaccg ccgaaggaat tcgcctgctc aatcaactta ttagcgcact
     1501 cttgagggtc ttgaaccttg ttcatccagc cttcgataat gtcatctgta agcattccac
     1561 aaagaccatc agaacaaaga agcaagcggt cgccttcttc gatatcaatc tcatattggt
     1621 cggcaaacat ttcagggtca ttgccaagtg ccctggtaat aagtgatctg tttggatgaa
     1681 cgcgagcctc ggcaggagta atttgccctt gctcaataag atcggccatc aaagagtggt
     1741 cgcgagtaat ttgttgcaat cttccgttgt gaagcagata agcacgcgaa tctccaactt
     1801 gagcaatgca aagtcggttg ccttcaataa cggccgcggt catggttgtg cccatgcctt
     1861 ccttgccctt gccttcaaga gccgccttaa taatttcttg gttggcgccc tcaacagcct
     1921 tggccaggcc ttcgcaatct gcctctcgag gtgcattttt acgcatgctt tcaattgcaa
     1981 tctccgaagc cacctcgcca gctgcatgtc ctcccatgcc atcggccaca acaaaaagtg
     2041 ggctttcagc aagaaggcta tcttcgttgt gctcgcgaac ttgacctata tcggttctgc
     2101 aaccatatct tatgtcttga tcaattttag atttaggcat atctaaccct aatgtcaacg
     2161 tctcctacac ttacagtatc tttatcacga aggttgtatg ggccttcgat aatttttcca
     2221 tttacagcgg tgccatttgt agatccaagg tcttcgatga acaaatcatt tcccatgagc
     2281 gaaaaccttg catgtcgacc acttacatat cccgcaccaa taacaatatc gcaaccggca
     2341 cttcgtccaa caataacagg accgtttact tgaatggaca caccacgaag ctccttcggg
     2401 cctcgttcaa ctgcgatatt ccaagtttta tctgccttac gctggccttt tacaagacca
     2461 atgccagttt tcataattgc aaataagaaa agatataaaa gcgcgattag cgcaagctta
     2521 caaattagaa ggataatatc aatcacgtca acctcgcttt tttagccaac gttaaaggtc
     2581 aacttcgtaa caccaattgt aattacatcc ccgggatgaa tttcgcgagt tgtaatttct
     2641 tggttgttaa caaaagttcc atttgtagat ccaaggtcat tgcaaagcca tagaccctgg
     2701 ttgtatgtaa tttgcgcatg ctgcctactt gcattagcat caggaagaac aatgttgcat
     2761 gtattttcgc ggccaataac aacagttgca gcatttagag tataaactct accagtagtt
     2821 tgctccatta aatgagctgg tggaatagct gcgttttgtt gaggcgcggc gtttgcattt
     2881 ggagtatcaa agtttcctgc aaatactgca gttttagcac ttgatacagg aacaggatta
     2941 gcgtttcctc ctaccgcttc tgcttgattc ggctcttgaa caggaacttc aacaggagct
     3001 ggcgtagcct gtggaaccgg agtaggttga ggagcaggtg taggctgcgg tactggagta
     3061 ttttgaactg ctggagctgg cgaagcaacg ccctgggctg gaacttcctc gaaaggcgca
     3121 aaaggactgc taacattttg aggagttgaa acctgaggag ttggattaac ttgggcttga
     3181 gcagcaactt ctgcagcagc ttgttgcgga gtttgagaca caacaggttg attttgattg
     3241 ttaatcccgt agcgctccat ctcttcggcg cgaagctgag taatgatatc tgcagctaca
     3301 ggctctgcaa ttacgtcaaa cttcccagcc ttaaggtcat tatcaacaat gaatcgaacc
     3361 aaagggtcac catccatagc aagcccagat tcgtttgcct ttgcttttag gtaagtttct
     3421 gtctcaccag ccaaagttgg ataatagcca aaaagtcgag catcatcatt tgcgttaacc
     3481 aaaacagtgt aaagcgttgg agcaaactgc ttcccagcgc ccaacatctt attacgacgc
     3541 atttgctttt ctgctttttt agcaatttga actggtgaaa ttggagaatc gaagaattta
     3601 tcgccaagat cgtcgatagc atcttccatc ttgttttcga actttgagaa aatacccata
     3661 attgctcctt ttaatttacg tttgtactaa ttcgcagttc tctcattctc gttaatatta
     3721 tctcatattt tcagcataaa ttacccatta taatatgatg aaacttgtct aagattttta
     3781 gattttatgt ataatttagg cgttaaaatt gacatgctaa attacaaaat taagtttata
     3841 atattgctcg gccccgtcaa gttggtaaat attgattaaa ttattcagac atatgaaaaa
     3901 acgcgaagta ctgatgattt tagtttcgtg cgtactaatt tgtggccaag tatatttcga
     3961 tttaaagatt ccagacttca tagacgagat gacaattctt atcaatactg ctggagctag
     4021 cgctaacaga acttgaatta ttggtgcgca aatgcttggg tgcttggctg caagtttggc
     4081 attaagtgtg ttgactggat acttagcggc gaaggctgcg gccgggttta cttttagtct
     4141 tcgtaaagaa ttgtttgata aggttatgag ttttggtgac gaagaaatta tgaagtttac
     4201 catcccaagt ttgatcacca gaactacaaa tgatatatcg caaattcaaa ctttgatatc
     4261 tatgggtttg caaattgctg ttaaggcacc aattactgct atatgagcta tatgcaaaat
     4321 tattggaagg aattgacaac tcactgccct taccggagct tttgttgtag ttgttgtagt
     4381 aagcgttttg attgtaatga gcatcgtgct tcctcgcttc cgcagaattc agaagttaac
     4441 tgaccgaatt aatttgcttg ttcgtgaaaa cctaagtggt attaatgttg ttcaagcttt
     4501 taacgctcaa gattatcaaa acgataaatt ccaagacgtc aacattgaaa tgacatttac
     4561 tcaattgttt aaccaaagag tatttgctgg acttcaacct attattgaac ttgcaacaac
     4621 tggtttatgg ttctctattt attggttggg tgccgcactt ctgattccaa tggctcttga
     4681 aaaccgcgtt gcattctttg gtgacattgt tgcttttgca atgtatgcaa actacatctt
     4741 gtccgcattt atgatgatgg ttatgatttt catgatgatt ccgcctgctc ttgcttcggc
     4801 agaacgtatt atggcagttc ttaacaccga agctcaaatt gccgaaggca agctcaacga
     4861 aaaagatgac aatgaaaaca tcatagaatt tagagatgtt tactttaaat atccaaacac
     4921 aagaaacgat atgctcaaaa gaattagctt ctcaattaag aaaggcgaaa ctgttgcgat
     4981 tatcggttca actggtagcg gtaaaactac tcttgttaat ttgatgatgc gcttctacga
     5041 tgcgacgacc ggtcatgttc ttcttaatgg ccaagacata aaagattata gctttgatac
     5101 tttatataac acaatttgta tggcaactca aaagccagtt attttctatg gaagcattga
     5161 tgacaatatt aaatttggcg aacacaataa agaacttacg gataaagaaa tcgaagaagc
     5221 cattgagatt gctcaagcac aagagtttat tgcaaagtta aaggataagc aaaaaacaat
     5281 tgttagccag ggtggtaaag atatttctgg tggacaaaaa caaagacttt caattgcaag
     5341 agcagttgca agaaaaccag aaatgctcat tttagatgat tcgtttagcg cacttgatta
     5401 caagactgac gcttcgcttc gtcgaatatt aaaagaaaaa atggctggca ctacaattgt
     5461 tatggttgct cagcgaattg gaacaattaa aaatgctgac aaaattttag ttttagaaaa
     5521 cggagaactt gctggaattg gcacacatga cgaattatta gaaagctgtg gcacatatca
     5581 agagattgca atttctcagt tgaggaaaga agaattggca aagataacaa agttaaagaa
     5641 aaatggcaaa taagaaaaaa gaagatacaa gtttcgacta cgcccaaaag aaagataact
     5701 ttcagggtaa taagggcatg aaaggcgctc ctactgatgg cggcgttaac atggtgtctt
     5761 cgaaagataa gcaaagcttt aaagatatca tctctactct tggaaaagtt ttcaaatatg
     5821 ttggtagaca caaaaaagtt ttgtacgccg gctttatttt agctgcagca agttcaattc
     5881 ttctaatgct tggtccaaac ttaattggac aaatggcaga cgcgattcaa aattcgctag
     5941 acaaagaaat tgacatactt acagtttcgg cgcttggctt tacacttatt gtcatatatg
     6001 gtttaagtaa tgctttcagt tttattcagc aatatattat ggctggaatg actgcgaagg
     6061 tttgcatgcg tcttcgttct gactttattc aaaagctaaa caagcttccg cttagctatt
     6121 tcaatactca cttgcaaggt gatattttat cttgcattac aaacgatatt caaactctac
     6181 gccaaggaat ttcaagatgt ttgcctggtc taattaaagc gcttgctcag tttttcacat
     6241 gcctcattat gatgattatc acagaatggc atctaacact ttgcgtttat ggagttgtgc
     6301 tacttggact tattgcaata atgactatca tgaagatgtc gcaaaagttc ttcgatcaaa
     6361 ggcaaagcaa tcttggtgct cttaacggtt tgattgaaga aatgtattca ggctatcaag
     6421 taattaaaat gtcattggca aagaaaaaag ttactgaaca atttgacgaa ggaaacgaaa
     6481 aactttacaa aacagattat atgtctcaat ttatttctgg aatcatggct ccaatgatgg
     6541 ttattattgg caacgttgca attttagtag taattgttgt tggcgcaaat ttggctatta
     6601 gtaatgtaat tacctttggt gctgttgctg catttctact cttttgtaat tattgcactc
     6661 aaccacttac aagaatcact caatacctaa ccgaccttca aggtgtttgt gctgctgctg
     6721 ttcgtctctt cgctttcttg gaagccgaag aaatgccaaa cgagcttttg aaggaaactg
     6781 aaattactga gcctaaaggc gaagttgaat ttgaccacgt taagtttagc tatccttcaa
     6841 atccagacaa aattattatc aacgatttct ctgcaaaagt taagcctgga caaaaagttg
     6901 cgattgttgg tcaaaccggc gctggaaaaa caacgctcgt taatttgctt atgagattct
     6961 atgacgttaa cagtggcgaa attaagattg atggaatacc tattaatgat ttgcgacgcg
     7021 acaatattca cgacatgttt ggtatggttt tgcaagaaac atggctcttc gaaggcaccg
     7081 ttcgtgaaaa cttagtttat aacttagaaa aagttccgga agagcgaatt attgaagttt
     7141 gcaaaacttg cggcgtttac gatttcatag tcaccatgcc agaaggtttt gacactaaaa
     7201 tgagcgaaag tcttgcaatt tcagctggtc aaaaacagct aattaccata gcgcgtgcaa
     7261 tgcttcaaaa tgcgccgatg ttaattttgg acgaagcaac atcttcggta gatacgagaa
     7321 cagaacttga catccaactt gcaatggata tgttgacacg aggaagaaca agcttcgtta
     7381 ttgcacacag actttcaaca attcaaaacg ctaacctaat cttggttatg gaagatggcg
     7441 acgttgttga atctggaact cacgatgaat taattgaact tggcggaaag tatttcgaac
     7501 tttacaatag tcaattccag gaatcataat ggcggctggt ccaaaaaata ctctagaagt
     7561 ggttgaggcc actatcaaaa agcatcacct ttttaagaaa acggactatt taattttgat
     7621 ggtttctggc ggaagcgact ctgttgctct ttcgtatatt atcagtgaac tcttccccaa
     7681 aaaatttgca ataatgcatc ttaatcataa tcttcgcatt gaggcaaacg aagatgcgca
     7741 atttgttgaa tctcttgcga agcatttggg cgctccatat tttggtttta gcgaaaacgt
     7801 ttcaggtgtg gcatcgcagg caaatgaaaa tatcgaagct tgcggacgag agcttcgtta
     7861 taaatatgca aacattgttg cttcaaagtt cagcggaaat gtaaaaattt gcacggcgca
     7921 taccgccgat gacaggattg aaaacttcta catgcgctca attattggca ctggtccagg
     7981 aggcttcgca agtatcaact acacaactaa caatgtgatt cgcccgcttc ttgaccttcc
     8041 aaaagaagca ctaatggact ttattaagtc atatcaaaat gcgtttaaag acagcagtgg
     8101 aaatttatgg cgcgaggata aaactaacga agacacagac cgctttcggg cttatgttcg
     8161 acatgaaatt attccgcttg caaaacgtca aaacccacaa atacttacaa acctcacaaa
     8221 ttccatgaac ttaattgcag atgaaagtga ctatattgaa gagaaggttt ccgagctgac
     8281 aattgagcac tttcaattta gcgacaattc atttttgatt cgtccaggat ttaaaaatga
     8341 aaacattgcg cttaaacgcc gtgcaattta taatgcgctt ctaaaagttt ttccaaaagg
     8401 aaccaggctt gaaaccaaaa gcattgcttc aatccttgag gcttcagaaa aatccaacta
     8461 cacggacaac atccaagaaa attattccgt tcactcaaat aaaaatggag ttctagtcca
     8521 gccaatggaa gaatatcgca aatcccgcaa ccgaatctaa aaaggtgtat ttcatgccag
     8581 gcaataatta caccttttgg tg
//
LOCUS       DBNCDC010000044         8522 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_3869_length_8522_cov_5.634581, whole genome shotgun sequence.
ACCESSION   DBNCDC010000044 DBNCDC010000000
VERSION     DBNCDC010000044.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8522)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8522
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3869_length_8522_cov_5.634581"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="AC04VQ_04295"
     CDS             <1..340
                     /locus_tag="AC04VQ_04295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIO9553384.1"
                     /translation="DIFVVTGSVNEQNKDVIKQIYDQMVEPKVVAACGACACSGGIFK
                     ECYNVQGGVDTVIPVDVYVPGCAVRPESIIDAVVLAIDALDAKAEKMKAERKAAKKGA
                     SKVEKEVNNG"
     gene            333..575
                     /locus_tag="AC04VQ_04300"
     CDS             333..575
                     /locus_tag="AC04VQ_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553385.1"
                     /translation="MAKFTQEFINIDLKKLHDEAASRCKNGWRYVQTLAVKVDGGTDL
                     IYSFMKDGKLENLKVQAVTEKDTVQSITDSYIEAFV"
     gene            669..815
                     /locus_tag="AC04VQ_04305"
     CDS             669..815
                     /locus_tag="AC04VQ_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553386.1"
                     /translation="MTIISPEVLERREKQAKIEAAMAAKKAKDAAEKKDDKPAEKKDE
                     GKGE"
     gene            817..1905
                     /locus_tag="AC04VQ_04310"
     CDS             817..1905
                     /locus_tag="AC04VQ_04310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HIO9553387.1"
                     /translation="MAKSSVIPFGPQHPVLPEPLHLDLVVEDEKVLQAIPQIGFVHRG
                     LEKLVDKKDYNQFVYVVERICGICSFGHSMGYSETIERLAKVEAPKRAQYLRAILLEL
                     SRVHSHILWMGLAADAFGYESLFMHCWRLRERVLDLFEKTCGGRVIMSVCAPGGMLRD
                     IDNSVLVEIKALLDSIKGEYTQLCKAFLKDESVKNRTVGVGVISKEEAEAFSMVGPFT
                     RASGVTYDVRSLGNGAYGDLDNFEPIIDDQGDCYARMKVRAVEVFQSIEIITELISKI
                     PDGEVFEPLKVNLEKDFEATNVLEQPRGECFYYGKGNGTKFLERFRIRTPTSQNLAGM
                     VTSLEGCDLADVNMIVLTIDPCISCTER"
     gene            1916..2305
                     /locus_tag="AC04VQ_04315"
     CDS             1916..2305
                     /locus_tag="AC04VQ_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIO9553388.1"
                     /translation="MGFFKLGTMTVGSVFKKPETLKYPFETKEPYAKQKGVIVNAKPT
                     ECTLCGICEKRCPANAIKVDREKRTWAINHLWCIQCDYCEQGCPKKCLVMKGERPGIT
                     ATKEDEVIKIPEKEKKVESEKPEDKKE"
     gene            2309..2593
                     /locus_tag="AC04VQ_04320"
     CDS             2309..2593
                     /locus_tag="AC04VQ_04320"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIO9553389.1"
                     /translation="MTKNAEDRILTVPNLISVIRILLIPVFCVLIFQQQNISAFIIFV
                     IACASDGVDGFIARHFNQTSELGKILDPIADRGLLLVGSVVLCIVGRLPI"
     gene            2687..2935
                     /locus_tag="AC04VQ_04325"
     CDS             2687..2935
                     /locus_tag="AC04VQ_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553390.1"
                     /translation="MGKIATTFFYIGFAILVLGDISWLTFPGLGIINASWLPGFSSEP
                     YSVGIWLIYIGILLNLYTSTHYIKVAISKLREAKNARN"
     gene            2922..3443
                     /locus_tag="AC04VQ_04330"
     CDS             2922..3443
                     /locus_tag="AC04VQ_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553391.1"
                     /translation="MPEINIQSTLNSNQTDYMKGLKKTWAFSQKLGDFTIPMKILILL
                     ARLLTFVFYSVYFLIKAIVLFIVGLCKTNKIVKWTVIILLVAGAIGLIDYVAFYGKIY
                     PGVYVGEVDLGGKTVEEAETLINDTYSERLNRNDVVIYVNDEAQAAGVQDEGASGIAE
                     ELSVEQAKQTVQY"
     gene            3465..3908
                     /locus_tag="AC04VQ_04335"
                     /pseudo
     CDS             3465..3908
                     /locus_tag="AC04VQ_04335"
                     /inference="COORDINATES: protein motif:HMM:NF023650.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
     gene            4164..5105
                     /locus_tag="AC04VQ_04340"
                     /pseudo
     CDS             4164..5105
                     /locus_tag="AC04VQ_04340"
                     /inference="COORDINATES: protein motif:HMM:NF016202.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
     gene            5108..5482
                     /locus_tag="AC04VQ_04345"
     CDS             5108..5482
                     /locus_tag="AC04VQ_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553392.1"
                     /translation="MTKRFKKAHLAALGVAVAIASALLAIALSVQAFAFTSSEDKLYG
                     ETLSSRGMSVKNCPNITAHYAVMIHQNGEVIYERNPYDHAKIASLTKIVTAIVALENS
                     EMTDEVVVTQKAYDIGESSAGV"
     gene            5501..6133
                     /locus_tag="AC04VQ_04350"
     CDS             5501..6133
                     /locus_tag="AC04VQ_04350"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIO9553393.1"
                     /translation="MLSALYALLVPSGNDAAEAICQSIGQKLVDCKDDRIRNKTKEEV
                     ASEAKEKGIAEEDVEDIFIDDNEQAFVRLMNLKAEELGCENTSFTNPHGLADEEFASD
                     DQYCCAMDIGVITRYAMQNETFRKIVGGGDTTITVDRKGQKVTLELHSTDILLGNFEG
                     CIGVKTGVTDIGGACFSGAMKDSDGDEIYTIVLKSDDETQRFVDTQNLFN"
     gene            6188..6589
                     /locus_tag="AC04VQ_04355"
     CDS             6188..6589
                     /locus_tag="AC04VQ_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553394.1"
                     /translation="MKDGEDEKEVGIVANLAHSEWVDCTFPVTLRDEDRGVPVLACAG
                     NVHLKIEPSEIKGSFKAGDKVAEIVLTQHNREAARLDLIAVKDQPGPNIFQMIGVAFD
                     RLGRNISGESCVADSEIFANCDKINELNAKS"
     gene            6602..7054
                     /locus_tag="AC04VQ_04360"
     CDS             6602..7054
                     /locus_tag="AC04VQ_04360"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIO9553395.1"
                     /translation="MSTKCPICLQEFEDGIEECPACGFKFSGNTEKFKPIKADNNEDS
                     KNTQGIDKKSNKTPALRVISGPQGELVLPLTKNNMTIGRSPNSDIFLNDRTVSREHAV
                     ISKNGDVYQIADQNSYNGLWINNNNVSVSNVKDGDLIQVGVFFLRFEA"
     gene            complement(7475..7921)
                     /gene="dut"
                     /locus_tag="AC04VQ_04365"
     CDS             complement(7475..7921)
                     /gene="dut"
                     /locus_tag="AC04VQ_04365"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006361471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIO9553396.1"
                     /translation="MGENSIKIPAKPLENCPGMPKYAYSGDAGFDLKSAEEAIIAPGE
                     RKVIHCGFAMAIPDGYAGLVIPRSGLAAKHGISVVNAPGLVDSGYRGEISVILLNTDK
                     NESFEISIGDRIAQMMIVPFANAELIESETLDSSERSEKGFGSSGR"
     gene            complement(7921..8367)
                     /gene="nrdR"
                     /locus_tag="AC04VQ_04370"
     CDS             complement(7921..8367)
                     /gene="nrdR"
                     /locus_tag="AC04VQ_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009307018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIO9553397.1"
                     /translation="MRCPKCGFEDSSVVDSRPTEDTSSIRRRRECLKCKHRFTTYERM
                     EEKPIVITKRGGSSEAFDRDKLFRGILISCAKRPVDPKDIESLIDEIVGELRNNGITE
                     IKSDDIGEMVLERLAKLDQVAYVRFASVYKEFGNVDEFKKVLKDLK"
BASE COUNT         2698 a         1553 c         1815 g         2456 t
ORIGIN      
        1 agacatcttc gttgttactg gaagtgttaa tgaacaaaac aaagatgtca tcaagcaaat
       61 ctacgaccaa atggtagagc caaaagttgt tgctgcgtgt ggcgcttgtg cttgcagcgg
      121 tggtattttt aaggaatgtt ataacgtcca aggtggggta gatacagtaa ttccagttga
      181 tgtttatgtt ccaggatgcg ctgttcggcc tgaatcaatc atcgatgctg tagtccttgc
      241 aattgatgct ttggatgcta aagcagaaaa gatgaaggca gaaagaaagg ccgctaaaaa
      301 gggcgcttct aaagtagaaa aggaggttaa taatggctaa gtttactcaa gaatttatta
      361 atattgacct caaaaaattg cacgacgaag ctgcttcgag gtgcaaaaat ggatggcgtt
      421 atgttcaaac actagctgtt aaagttgatg gcggaactga tctaatttat agctttatga
      481 aagatggcaa acttgaaaac ctaaaagttc aagctgtcac cgaaaaagac acagttcaat
      541 ctattacaga tagctacatc gaagcttttg tttgagaaaa cgaaattcat gatttgtgaa
      601 atatcaactt taaagatatt gcaattgact ttaaaggaac attttacaaa gtagcaacag
      661 acgctccaat gacaattatc agtcctgaag tgcttgaaag aagagaaaag caagctaaga
      721 tcgaagctgc aatggctgct aaaaaggcta aagatgctgc tgagaagaag gatgataaac
      781 cagcggagaa aaaagacgaa gggaaaggtg agtaatatgg ctaaatcaag tgttatacct
      841 tttggacctc aacatccagt tttgccagaa cctcttcatc ttgaccttgt tgtcgaagac
      901 gaaaaagttc tacaggcaat tcctcaaatt ggttttgttc acagaggcct tgaaaagctt
      961 gttgataaaa aagattacaa tcaatttgtt tacgttgttg agcgtatttg cggaatttgt
     1021 tcttttggtc attctatggg ctatagcgaa accattgagc gcctagctaa ggtcgaagct
     1081 ccaaaacgtg cgcaatacct tcgcgcaatt ttacttgagc tttctcgtgt ccattctcat
     1141 attttatgga tgggtcttgc tgcagatgcc ttcggttacg aaagtttgtt tatgcattgt
     1201 tggcgtcttc gcgaacgtgt acttgacctc ttcgaaaaga catgtggagg ccgtgtaata
     1261 atgagcgttt gtgctccagg tggtatgctt cgtgacattg acaattctgt tcttgtagaa
     1321 attaaagctc tgcttgacag cattaaaggc gaatatacgc aactttgcaa ggctttctta
     1381 aaggatgaaa gtgttaagaa cagaactgtt ggcgttggcg ttattagcaa agaagaagcc
     1441 gaggccttca gcatggttgg tccatttaca cgtgcttctg gtgttactta tgatgttcgt
     1501 agcctcggaa acggtgctta tggcgatctt gacaattttg aacctattat tgatgaccag
     1561 ggcgattgct atgctcgtat gaaggttcgt gcagttgagg tattccagtc catcgaaatt
     1621 atcacagagt taatttcaaa gattccagac ggcgaagttt ttgagcctct caaagttaat
     1681 cttgaaaaag atttcgaagc aacaaacgtt cttgaacaac caagaggaga gtgcttctat
     1741 tacggaaaag gcaacggaac gaagttcttg gagcgcttta gaattagaac acccacgtca
     1801 caaaacctcg ctggtatggt tacttctctt gagggctgtg atctggcaga cgttaacatg
     1861 atagttctaa caattgaccc gtgcatttct tgcacagaaa gataggaggc tggctatggg
     1921 atttttcaaa cttggaacaa tgaccgttgg ctcggtcttc aaaaaacctg aaacactaaa
     1981 gtatcctttt gaaacaaagg agccttacgc gaaacaaaaa ggcgtgattg ttaatgcgaa
     2041 gccaaccgaa tgcactcttt gcggtatttg cgagaagcgt tgtcctgcaa atgcaattaa
     2101 ggttgataga gaaaaaagaa cctgggcaat taatcattta tggtgcattc aatgtgatta
     2161 ttgcgaacaa ggttgtccta aaaaatgcct tgttatgaaa ggcgaacgtc ctggaattac
     2221 agctactaaa gaagacgaag taattaaaat tccagaaaaa gaaaagaaag tagaaagcga
     2281 aaaaccagaa gacaaaaagg aataactaat gactaaaaat gcggaagaca gaattttaac
     2341 ggttccaaac cttatttctg ttatccgcat tttacttatt cctgtttttt gcgttctaat
     2401 atttcaacaa caaaacattt ccgcttttat aatttttgta attgcttgcg caagcgacgg
     2461 cgtagacgga tttatagcaa gacactttaa tcaaacaagt gagcttggaa agattcttga
     2521 ccctattgca gataggggac tattgcttgt cggatctgta gttttgtgta ttgttggcag
     2581 gctgccaatt tgaatcgttg ttttagtcat cgccagagat tttacctttt tattcggcgg
     2641 atgatatgta atgaaagttg cggattatag gccaaaagta atctgaatgg ggaagatcgc
     2701 aactactttt ttctatatag gttttgcgat tttagttttg ggtgatattt catggctaac
     2761 ttttccgggg cttggaatta taaatgcttc gtggctgccg ggcttttctt cggagcctta
     2821 ttctgtagga atatggttaa tttatatagg cattttgctt aatttgtaca catcaactca
     2881 ttatattaag gtggcaattt ctaaacttcg cgaggccaaa aatgccagaa attaatatac
     2941 aaagcacact taatagtaac caaacggact atatgaaggg ccttaaaaaa acatgggctt
     3001 tttctcaaaa acttggcgac ttcacaatcc cgatgaaaat tttaattctg ttggcaaggc
     3061 ttttgacgtt tgtgttttat agcgtttact ttttgattaa agctattgtg ctattcatag
     3121 ttggtttatg caaaacaaat aaaattgtta aatggaccgt aataattctt ttggtagctg
     3181 gggcaatagg gcttattgac tatgttgcat tttatggaaa aatctaccct ggagtttatg
     3241 tgggcgaagt cgacttaggt ggcaaaaccg tcgaagaagc tgaaactcta attaacgaca
     3301 cttattcgga gcgtcttaac agaaatgatg ttgtaattta tgttaacgac gaagctcaag
     3361 ccgctggcgt tcaagacgaa ggtgcttcgg ggattgccga agaactaagc gttgaacaag
     3421 caaaacaaac cgttcaatat tgaaagactg atgcagttga gcttgaagca agtcttccaa
     3481 ctgctgacct tgttgataag gcagcaaatt caacaagagg ccttctaaat attttttcaa
     3541 ggctgggcgc tttgttattc aagcaggtaa ttgagcctta cgctgacttt aacgattctg
     3601 ctatccaatc acaaatttca agggtaaatg acacgatagg aaaagaggtc gttaatccaa
     3661 cttgcgttat cgaagaaggt caagccgttg ccaaggaagg aaacgatggt ctgcttgtca
     3721 acaacgatga atttatcaaa caaataaaca aagccttctt tgaggcagat aaagaaaata
     3781 gccattttgt tccaaagctc tacgttgcaa agcagcaagt ttcttttgcg gcggctaata
     3841 ccttggccca aaaaatcaat acttcgcttg aaaaagaagt tagttttgac tataaaggca
     3901 acgaatgaaa ggttgacaga ctcaatttat gcaattgaat tactgtcgaa gttcaagata
     3961 gaagcaacga agctaatgca ggttgctcct gcgcttcgat gtttggaccg gtatattgta
     4021 tttgtgctaa ggttgacagc aatcaagctg gagcagatat tttagttcac actcgcgaac
     4081 aaaacatgga agctacggac tattcaatac agatggctaa ggatactaag ggcattacgg
     4141 taaagacaca aggcaacttt gaaatgccag caatttatga tgctgttgat tctctcaacg
     4201 aagaatggct taacaacgct gctgtgttcg accgagattt agctggtgct tcggggagca
     4261 atgaaactga tcctgtccat ataaagattg gcgcaacaaa ggtccctgaa accttaactc
     4321 ttgatgaagc cattagaatt ggcgtcgttt cggagatttc gacatataca acaacttatt
     4381 caagtggtgc cggaaccgag aacagaaaca agaatattgc tttgggggca gctgctctaa
     4441 acaatacaat atgcagcgcg aatggcggcg tttggtcttt taacgatgtt gttggaaata
     4501 ctaccgaaga taagggctac cttgaagctg gtgcgatatg aggaaacaga tatacaaaat
     4561 cagttggtgg aggaatttgc caggttgcta caactgtttt taatgcagtt tacgattctg
     4621 gccttgcaat tccagaacgc tacaatcatg accttcacat cgcaagttat cctgtaggaa
     4681 gagatgccgc gatcaactat cctgacttag accttgtttg gcgaaatgat gaaaagtcag
     4741 atattttgtt gacatcttct actacaggct attcaattac tgtaagtttg cttggtgttc
     4801 atccaggata cacagttaaa accgaagtag gcgagtggac tccgggtgag ccttataagc
     4861 aagaatttgt tgttgatcct gctaaagaac ttaattcaaa gaaagttcaa acaagtgggt
     4921 ccgacggcaa aagcattgta attcatcgat ttgtctacaa cagaaatgga caacttgttc
     4981 gtcaagatga atttaaatca aactacaagc caaaaagcga aatagttgtt cttggtccag
     5041 gtgacgaagc caacaaattg cttgctcaac acttggcaag aatgaaagat gaaacggata
     5101 attgataatg actaagcgat tcaaaaaagc tcatttagct gcacttggag tagctgttgc
     5161 catagcttcg gcacttttgg caattgcact ttctgtccaa gcttttgctt ttacaagcag
     5221 cgaagataag ctatacgggg aaactttaag ctctcgagga atgtctgtta aaaactgtcc
     5281 taatataacg gctcattatg cggttatgat ccatcaaaat ggagaagtaa tttatgagag
     5341 aaacccttat gatcatgcaa aaattgcctc gttaaccaaa attgttacag cgatagttgc
     5401 gctagagaat tccgagatga ctgacgaagt tgttgtaact caaaaagcct atgacatagg
     5461 ggagagttct gctggtgttt gaccagatga caaacttgat atgctaagcg ctctttatgc
     5521 cttacttgtt ccttcgggaa acgatgctgc agaagcaatt tgtcaaagca tcggacaaaa
     5581 gttggttgac tgtaaagatg atcgcattcg aaataaaaca aaagaagaag ttgcaagcga
     5641 agctaaagag aagggcattg ccgaagaaga tgttgaggat atctttatag atgataacga
     5701 gcaagccttc gttcgtctta tgaacctaaa agccgaagag cttggctgtg aaaatacgag
     5761 ttttactaat cctcatggct tggctgacga agagttcgcg agcgatgatc aatattgttg
     5821 tgctatggac attggtgtaa ttaccaggta cgcaatgcaa aatgagacct ttagaaaaat
     5881 tgttggcgga ggagatacaa caattacggt tgatagaaaa gggcaaaaag taacgctcga
     5941 gcttcactcc acagatattt tgctgggtaa ttttgaagga tgtattggcg ttaaaactgg
     6001 agttacagat attggtggcg cttgctttag cggggcaatg aaagattctg acggggatga
     6061 aatctataca attgttttga agtccgatga cgagactcag cgttttgttg atactcaaaa
     6121 tcttttcaat tgatatttac aaaataagat caagcttgac ctaaccgata ccggaataaa
     6181 agacacaatg aaggacgggg aagatgaaaa agaagttgga attgttgcaa atcttgctca
     6241 ctctgaatgg gtcgattgca cctttccggt tactcttcgt gacgaagata gaggtgttcc
     6301 tgttcttgca tgtgccggaa acgttcatct taagatagag ccatcagaga taaaaggaag
     6361 ctttaaggca ggcgacaaag ttgccgaaat agttttgacg cagcacaaca gggaagctgc
     6421 aaggcttgat ttaatagccg ttaaagacca accaggccct aatatctttc agatgattgg
     6481 cgtagctttt gataggcttg ggcgaaacat ttctggagaa agttgcgtag ctgacagcga
     6541 aatcttcgca aattgtgata agataaacga acttaacgca aagagttaaa ggggagttgt
     6601 catgagcaca aaatgtccaa tttgtttaca agaattcgaa gacggaatcg aagaatgtcc
     6661 tgcttgtggc tttaaattca gtggaaatac tgagaaattt aagccaatta aagcagataa
     6721 taacgaagat agcaaaaaca cccagggtat agacaaaaaa tccaacaaaa cccctgctct
     6781 acgcgttatt tcgggacctc aaggtgaact tgttttacca ctaacaaaga acaatatgac
     6841 tatcggacgc agcccaaaca gcgatatttt ccttaatgac aggactgtat cacgcgaaca
     6901 tgctgttata tccaagaatg gggacgtata ccaaattgcc gatcaaaact cttataacgg
     6961 cctctggatc aataacaata atgtttctgt atctaatgtt aaagacggag accttattca
     7021 agtaggcgta ttcttcttac gctttgaagc ctaaaagatg taaatattgc caggcaaaaa
     7081 tttcaccttt ttaaataatg tatgattagc agggtatata cacctatatt aaaactttct
     7141 tctactttac ataacatata ttatcatact tttgccataa atagtatagt aatgtttgag
     7201 caaaagtatt gataatataa gtaccacttt aaagcttgat cagacggctg ataatataat
     7261 acaactgttt cattaatact cgatttcccc ggggcccaca ctcgatttgc tcgtggcatt
     7321 tactcgattt actcgtggca tttaattgat ttttgaaaat tatagaaaat taagaattta
     7381 tgaaaaatta aatgattttc caaggtataa atactcaagg ttgaaaataa aacggcctta
     7441 aaacgcaaat aaaagcctcg ttttacctgg ggttttagcg acctgaagat ccgaagcctt
     7501 tttcgcttct ttcgcttgaa tctaaggttt cagattcaat taattcggca tttgcgaatg
     7561 gaactatcat catttgagcg attcggtcgc ctatagatat ttcaaaactt tcatttttat
     7621 ctgtatttaa caaaattaca gaaatttcgc cgcgatatcc gctatcaacc aatcctggag
     7681 catttaccac agaaattcca tgttttgcag ctaatccgct tcgtggtatt acaagacctg
     7741 cataaccatc aggaatagcc atagcaaagc cacaatggat tacttttcgc tctccaggag
     7801 caattatcgc ctcttcggca cttttgagat caaatccagc atcaccagaa tacgcatatt
     7861 taggcattcc aggacaattt tctagtggtt tagctggtat tttaatggaa ttctcgccca
     7921 ttattttaga tcttttagga cctttttgaa ctcgtcaacg ttaccaaatt ccttgtatac
     7981 gctggcaaaa cgcacatatg caacctgatc gagcttcgca agacgctcaa gaaccatctc
     8041 gcctatgtca tcagacttaa tttcagttat tccgttgttt cgaagctcac ctacaatctc
     8101 atcaataagc gattcgatgt cttttggatc caccggacgc ttcgcacatg aaattaaaat
     8161 tccgcggaaa agtttatctc tgtcaaaagc ctccgaagat ccaccgcgct tcgtaataac
     8221 aattggcttt tcttccatac gctcataagt agtaaaacga tgcttgcatt ttaagcactc
     8281 tcgcctacga cgaatcgacg aagtgtcttc ggtcggacgc gaatcgacaa cgcttgaatc
     8341 ttcgaaacca catttaggac aacgcatttt taccccttta aaaactacat attgtgtaat
     8401 ttgcaataat gttgggctat tctaccataa tatttatact aaatatagat aatttgttca
     8461 gaaaataaat ttttaattta ttgcacaaat gtttgataaa gtgctaacat aagcccccca
     8521 tg
//
LOCUS       DBNCDC010000045         8436 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_3922_length_8436_cov_7.569980, whole genome shotgun sequence.
ACCESSION   DBNCDC010000045 DBNCDC010000000
VERSION     DBNCDC010000045.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 8436)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8436)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8436
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3922_length_8436_cov_7.569980"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(1..2445)
                     /locus_tag="AC04VQ_04375"
                     /pseudo
     CDS             complement(1..2445)
                     /locus_tag="AC04VQ_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(2464..3549)
                     /locus_tag="AC04VQ_04380"
     CDS             complement(2464..3549)
                     /locus_tag="AC04VQ_04380"
                     /inference="COORDINATES: protein motif:HMM:NF022779.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HIO9553398.1"
                     /translation="MIFLFVTLVASCTIWPVIGPFYETIRRNILVKNQDFSLNAILKT
                     IGKIILSALPITFAFFGGILINNEIIMKIIFGGGFVGSSVIVMLFLAKRTRSINAGIR
                     LGCKANPNIEAVWHNKITAHDIMDCVSCDSSSLTYNFVIKHDIADVSFSTANFRTATS
                     TNMGSADHAEVVHYKISKKCKSPIICTFDKELTDSPLPIVETENPAFNKEFTTFCEKD
                     DDAFYILTPQVMEKMIDAAKTYKRVIYNFDEDDLFIIIYHDKFLDALKKNDVSGAFDG
                     MKQLINDLVYKNIAKPGAHVKATPLSKDDPDKFTTEAEELASQGIKITQKPLQVNSKK
                     AVAFFVGIFAVVIISNIVIFGIVFYFI"
     gene            complement(3819..4103)
                     /locus_tag="AC04VQ_04385"
     CDS             complement(3819..4103)
                     /locus_tag="AC04VQ_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553399.1"
                     /translation="MAINSNIDHLYFASGPFTDKFNSIEDFLNKKTDLYISIYLKESF
                     GEINADNSLELSKQIVATTKIKDAYFNYYNENDRNKQCAYRYKNGEYYIS"
     gene            complement(4182..5507)
                     /locus_tag="AC04VQ_04390"
     CDS             complement(4182..5507)
                     /locus_tag="AC04VQ_04390"
                     /inference="COORDINATES: protein motif:HMM:NF022779.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HIO9553400.1"
                     /translation="MYAESGEWIIQHFASTIGSTIFPTVVFAFMFGPMFERVKKNVLV
                     RNQDRSIYSLLRTFGKIFICIAPFLFCFIMSKLGISSRIADPILMLGVAVSLIISLIF
                     FKIRNGNIEKGIREGCKKFPNFESVAQNRINEREIIDCITCDSVETEHNFVIKNDITD
                     VGFSCVNFKTIVGKRGSSVTDPAEAIHYKLSKKCKSPIICTFDEEITDSPMPIVETEN
                     PAFNNEFTTYCEKASDAFYILTPQIMEQMIEAQKIYKRLVFNFDGDNLYIIIYHDKFK
                     RALAKKDFDAAFNGVNEMLGYLIFDSIAQAGAHKEKARKAKDLISEETKVKNEAVTRA
                     SSSRFKKGLKIGALVLGAIIVLNLFFLTLSFCAFNESTVRDTSSGTNSAPTTEEISKI
                     QTYLKQKYGQDFAAPSTSYYSKYESNYYTRVSGQFSDANGYSFYAQSQL"
     gene            complement(5727..6275)
                     /locus_tag="AC04VQ_04395"
     CDS             complement(5727..6275)
                     /locus_tag="AC04VQ_04395"
                     /inference="COORDINATES: protein motif:HMM:NF015944.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIO9553401.1"
                     /translation="MSTGMIILIVVVAVLLIIAIWAISVNNAVKRAGLKCDEAMSGID
                     VALEKRHDMLTKLLDTAKKYAEHEKSTILKSVELRKDAPIAQKVENANKLDSAQNYLF
                     GLMENYPDLKASVNYEELQQGIMDAEEHLQASRRAYNAAATQYNNYHVTFPNSLIANR
                     IKPEKREYFEASAEKKEDVKIK"
     gene            complement(6468..7226)
                     /locus_tag="AC04VQ_04400"
                     /pseudo
     CDS             complement(6468..7226)
                     /locus_tag="AC04VQ_04400"
                     /inference="COORDINATES: protein motif:HMM:NF020892.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
     gene            complement(7386..8306)
                     /locus_tag="AC04VQ_04405"
                     /pseudo
     CDS             complement(7386..8306)
                     /locus_tag="AC04VQ_04405"
                     /inference="COORDINATES: protein motif:HMM:NF020892.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
BASE COUNT         2561 a         1725 c         1470 g         2680 t
ORIGIN      
        1 aattattatc gaaactggat tttcaagatc ggcataaccg aaatctgcga gattgccttc
       61 gtaagatctg ctgccagaac cactcataag tttggcaagt tgcatcattt gatctgatga
      121 aatgttgaaa tccatatcga tgctgttcat agcagacatc attttttcag cgattgcggt
      181 tgtaacacca tgaactacct gaccaacaat gccttgaatg ccttcaacaa ttgcaggtgt
      241 cgaagctata cttgccgccg cacaaatcat tgcaatctga ttttgacctt cctctgttgc
      301 gaaataaatt tcaggggttg cttctgaagg tgttggttgt gcggctgccc aaactaaata
      361 caaatctgtt gtgagaatta tggcattctt tacttctgga ttttttaaat caagaattgt
      421 gcaattctca ataattttgc cgatggcttc gctagacatt gcggttttaa taattccaac
      481 aatgtcaatg tctttcatac ttgatgcgtc aaaggtaaca gcaggcataa aatcggaagt
      541 gtcaaaaccc atgtctttct tttcttcgac aaatgtttta ccagtaagaa cgtcaacttc
      601 gggcttagac atttgatctt taacaatttg acttgtgcca gcagcgttga tgcattcttc
      661 gtttaagtca taagaaaaat taattccagg actaagcgaa agagcaacgt tgcttccttc
      721 tggtgggtta acaattccaa caatctttaa atccttgccg gcggacacaa cttgttgcgc
      781 aaaagcttcg tttgaagact tgtcaatcca aacgcccttc gcactgttgt attcatacat
      841 atcataggcg ttgacaattt tgaaggtgag gccgagcaag tcatcgtagc ttaggtcatc
      901 ataattttca ggcaagttaa ccttttcatc tttcatgaaa ttgtcgagcg catcttccat
      961 ttctttctta tcgcgaaaac ccaaaatgta gagcatcaaa tcataaattg ttccatcctg
     1021 gtttgtgacc aaaacgcatt catgagaatt ttcaggccaa tgacctgcag caacggtgta
     1081 gacattctta taaaggctct cactttttgg aagacttcca aaaccactaa gcgaactcat
     1141 tccaccactg ctgtaatttt gcgaagccga agtatagccc gaacctgttg tgccaattgt
     1201 tgtggatgtt aaagtccccg aaggaaagac ttcgcggact ccatatttat cattggcata
     1261 gatgattggc tgagcacgat aggtgtattc gattgaatca acattttcca tcatgtcggt
     1321 cgaattcgaa tctatgaatt ttttgagaga cttaagatca ttgtcgccac ttgttgcaag
     1381 catctttgat agaaaagttc taacgccaat ttttccggct tcttgattat tatggcgcat
     1441 ctcttgaatc atgtttgaaa ggcctccact gcccttcgcc atgtcagtaa attcactaac
     1501 aaaagatttt gaacgagtaa tgtagagcgg atattgcgaa agcgtattct tttgaacgtg
     1561 atcaatgtat tcattagtgc ctgttgagag tgctaaaatt gctgaaattc caatgatgcc
     1621 aatgcttcct gcaaaagccg tcataaaggt gcggcctttc ttacccatca aatttgcgaa
     1681 ggaaagcgag agcgcagttt taaaactcat cgcagttttt cctttttcaa gaagcttacg
     1741 gaatttattc gcgccatctt tttttggctt ataaggtttt gaatcaccaa caattttggc
     1801 atccttcaaa ttaacaattc gcgtagcgta tttctcagca atttcagggt tgtgcgtaac
     1861 cataacaaca agacgatcct tcgcaatttt tttaaggatg tccataattt ggatgcttgt
     1921 ttttgtatcg agcgcaccgg tcggctcatc cgcaaaaaca atgttagggt cgtttacaat
     1981 cgcacgagca attgcaacac gttgttgctg accgccggaa agttgattcg gcttcttttc
     2041 gatgtgctcg cttaaaccaa cttcatccag ggctttcttc gcacgttctt tgcgctccga
     2101 agtcgaaatc cccgaaagtg taagcgcaag ctcaacatta gaaagcacac tttgatgcgg
     2161 aattaaattg taattttgaa aaacgaagcc aacttgattg ttgcggtaat ggtctcaatc
     2221 ttgatcctta aattctttgg tggacttacc gttgataagc aaatcgccac tatcataatg
     2281 atcaagaccg ccgatgacat ttaacatcgt tgttttacca ctaccacttg gaccaagcac
     2341 cgccacaaat tcgctatcac gaaacgtgac cgaaacttta tcaagcgcct tttgcgtaaa
     2401 gtcgccagtg gtataagact tcgatatttc ttttaactct aacattaggg tgttaattat
     2461 agattatata aagtagaaaa caataccaaa aatgacgatg ttggagatta tcacaacggc
     2521 aaaaattcct acgaagaacg ccactgcctt ctttgagtta acttgaagtg gtttttgagt
     2581 aatcttaatt ccttggcttg ctagttcttc ggcttctgta gtgaatttat cagggtcgtc
     2641 ttttgagaga ggtgtggctt taacatgagc gccaggcttc gcaatgtttt tataaacgag
     2701 gtcattaatc aactgcttca taccatcaaa agcaccgctt acatcatttt tcttaagagc
     2761 atctaaaaac ttatcgtgat aaataatgat aaaaagatca tcttcgtcaa aattataaat
     2821 tacacgctta tacgtttttg cagcatcgat catcttttcc ataacttgag gagtcaaaat
     2881 atagaaagca tcatcgtctt tttcgcaaaa agttgtgaac tccttattaa aggcagggtt
     2941 ttcggtttca acaattggaa gcggcgaatc tgtaagctct ttatcgaatg tgcaaattat
     3001 cggactttta catttcttcg aaattttata atggacaact tcagcgtgat cggccgagcc
     3061 catatttgta gaagttgctg ttctaaaatt cgcggttgaa aaactaacat cggcaatgtc
     3121 atgcttgata acaaaattat aagttaacga agagctatca caacttacac aatccataat
     3181 gtcgtgagcg gtaattttat tgtgccaaac tgcttcgata tttggattgg ctttacatcc
     3241 caaacgaatg ccggcattga tgcttcgagt gcgcttcgcc aaaaaaagca taacaatcac
     3301 tgacgaacca acaaaaccac caccaaatat aatcttcata ataatttcgt tgtttattaa
     3361 aattccacca aaaaatgcaa acgtaattgg aagagccgaa agtataattt taccaatggt
     3421 ttttaaaata gcattaagtg aaaaatcttg atttttgacc aaaatatttc gtcgaattgt
     3481 ttcataaaat ggaccaataa ctggccaaat tgtacatgac gctacaagag taacaaaaag
     3541 gaaaatcaaa aaactttcag gtcgtcaaaa accaagcatg ttgtactcag taataacaat
     3601 atattctgca acagccgtac caattattaa tccaaccatt gcggagtaaa caattcttcg
     3661 tttaagtcta aatctcgaat tatttgttaa cctgtcgttt gcccttttcc tcgtgtatcg
     3721 ttttttgtaa gttgctaaaa cataaacggc aacaataatt attacaacgc ctccaataag
     3781 tggtattcaa agattgttaa acacctaagc tccaatcatt aagaaatata gtattcgcca
     3841 tttttatagc gataggcgca ttgcttgttc cggtcatttt cattgtaata attaaaatat
     3901 gcatctttaa ttttagttgt tgcaacaatt tgcttcgaca attccagaga attatcagca
     3961 ttaatttcac caaacgattc cttcaaataa attgaaatat acaagtcagt ctttttattc
     4021 aaaaaatctt cgatgctatt aaatttgtca gtaaaaggac cacttgcaaa atataggtga
     4081 tcaatatttg aatttattgc caatatttgt tgctcaataa ttttgtcata ctgcgaagat
     4141 tgataatcgt cactaaactc aattccttgg ctatcatcat ttcataattg tgactgcgca
     4201 taaaatgaat aaccatttgc atcactaaat tgacctgaca ctcttgtgta ataattactt
     4261 tcatacttgg aataatacga agttgaagga gcagcgaaat cctgcccata cttttgcttt
     4321 aaataagttt ggatttttga aatttcttca gttgtaggtg ccgaatttgt tccgcttgac
     4381 gtatctcgga cagtactttc attaaaagcg caaaatgaaa gagtcaaaaa gaaaagattc
     4441 aaaacaatta ttgcgccaag aacaagggca ccaattttca gtcccttttt aaacctcgaa
     4501 gaactggcac gcgtaacggc ttcgttttta actttagttt cttcgctaat taaatcctta
     4561 gcttttctgg ctttttcttt atgggcgcca gcctgcgcaa tactatcgaa gataaggtaa
     4621 ccaagcattt cgttgacgcc gttaaatgca gcgtcgaaat cttttttagc taaagcgcgt
     4681 ttaaatttat cgtgataaat aattatgtaa agattatccc catcgaagtt gaaaacaaga
     4741 cgcttgtaaa ttttttgagc ctcaatcatt tgctccataa tttgtggtgt caaaatataa
     4801 aatgcatccg aagctttttc acaataagtt gtgaactcat tattgaaagc aggattttca
     4861 gtttcaacaa ttggcattgg agaatcagta atctcttcgt caaatgtaca aataataggg
     4921 cttttgcact ttttcgaaag tttgtaatga attgcttcgg caggatcagt caccgaagaa
     4981 ccgcgcttgc ctactatcgt tttaaaattt acacaagaaa aaccaacatc agttatatca
     5041 tttttgatga cgaaattgtg ctcagtttcg acggaatcgc aggtgataca atcaataatt
     5101 tcacgttcat taattctgtt ttgtgcaaca ctttcaaaat ttggaaactt tttgcatcct
     5161 tccctaattc ccttttcaat atttccgttt ctgattttaa aaaatataag tgaaattatc
     5221 agcgataccg ccactcccaa catcaaaatt ggatcagcaa tcctcgaact tatacccaac
     5281 ttagacataa taaagcaaaa caaaaacggc gcaatgcaaa taaaaatttt gccaaatgtg
     5341 cgaagaagcg aataaattga gcgatcttga ttacgcacca aaacattttt tttgacgcgt
     5401 tcaaacatag ggccaaacat aaatgcaaaa actacagtag gaaaaattgt gctaccaata
     5461 gtcgaagcaa aatgctgaat tatccattct ccagattcag catacataac agatgtgcta
     5521 aattctgcaa taactgttcc aataataatt ccaactaaaa cagaatttat taacctacgt
     5581 ttaattttgt atcatgcatt attttgttct cgtgattttg ctctcttttt tgtacgagtg
     5641 tttcgatact taaaaagagc aaaaacacaa agaacaatta tgactgttcc aataaggaac
     5701 gctattaaaa gattttcaga catttattat ttaatcttaa cgtcttcttt tttctcagca
     5761 gaagcttcaa aatattcacg cttttctggc ttaatgcggt ttgcaatcaa gctatttggg
     5821 aaagtaacgt gataattatt gtattgtgta gcagctgcgt tgtaagcacg acgtgaagct
     5881 tgaagatgtt cttcggcatc cataatgccc tgttgaagtt cttcgtagtt cacacttgcc
     5941 tttaggtctg gataattttc catcaaaccg aagaggtagt tttgcgcact atcaagtttg
     6001 tttgcatttt caactttttg tgcgattggt gcatccttac gaagctcaac agactttaag
     6061 attgttgact tttcatgctc agcatacttt ttagctgtat ccaaaagttt agtgagcatg
     6121 tcatgcctct tttcgagggc gacgtcgatg ccggacattg cttcgtcaca ttttaagcct
     6181 gcacgcttaa cagcattgtt aaccgagatt gcccaaattg cgatgatgag caaaacagca
     6241 actacaacaa ttaaaataat cattcctgta ctcataattt ttcctttcta cttatattga
     6301 ctttcgtgct tgcgaaaaaa atcaaatttt ggtggcaact cttttacccg aagaattctt
     6361 gtttcgttca tcgaataagt tggtgttgcc gaagttgtgc tcaagctttc tctaacttga
     6421 acattgtaaa gtgcaacctc atttagttcc gtctcataga agctttttca actgaaaaat
     6481 ttagcgagtg gaatattcat gcattctgca attttttcac aaccgcgtgg aacaatagga
     6541 tgcatcatca aagctgcagc atatagcatg aaaaacgaat tgctaagaaa attgcgcaag
     6601 aacaattcgt cgttctcttc gatttttgaa gcgttgtctg aaaagtattt attagcataa
     6661 cgaataaatt catccatcaa ttgcatgact gaataaagct cagtcttttg catgtatcgc
     6721 tcatactttt cggcagtctc taaaactgtt gttgcaactt ccttatcaac cggcatgccc
     6781 ttaacttggc aatccgaata tttttgagca gtatagaaac acgaacgagc aaacctgtta
     6841 aacacatttg taattaaagt gccttctttt aaaactgggt ctgccgcacg tggatcaagg
     6901 cgcttctcct tgtctttatc gaatggcttt ggcgaaaatg aaacagattt ttgcgcaaga
     6961 ccaagagcca accaatgagc tctaagctga tcagaggtgt aataatccaa tagctcatct
     7021 gcacttggag gcttaacttc gccagaagac gaagccttag tatttccaaa caacaaatga
     7081 tgattagcaa ccaaagtagt ttgacgaagc ccagaaaaac tgttcttcgc gtttctaccc
     7141 ttgctattag tttgaaatgc atcaaacaat gcgggttgcg caacaccata aaaataaata
     7201 ttgtcttgac caataaattg atagacttgt gattcatcgc ttgctcaaaa atcttttcaa
     7261 tcaaattttg atttcaattt ttctgtagcg gccatcgtga aagaaattgg agcccataaa
     7321 ctttccggcc aacaccaaac agtaaggcct tcgctttcgc catctaagtt tggtgcttta
     7381 acgcctcatt cagcattgcc tgtaagtcta aatgggacaa gcgtcttacc tgttcggaag
     7441 cgaattccag cctcggccag aacttcgcga gccgcatctc tgtcttcgat agttttaaat
     7501 tcgatctgaa aactttgttt attttcttcc gcctcacgaa acttatgctt aggcaaattt
     7561 gattcaagtt ttttgtaatc atcgaagcat tcaactttta caaaaattac aggaggcgcc
     7621 aaaaattcgg caatatcatt tgaaacaaca tcgcgaacat tttcatcctt gcgcataaat
     7681 tcaacttgcc tttgcaaaaa ccttctatag gccggcaaat cgaaatacca atttctaaca
     7741 gttttcattt ctggctcaac gccagtcacg cttgactttg gagcaatcaa atcttgaggc
     7801 gcataactgt gtccaagact gcattcatca gcataggctt gctcactctt acaacctgct
     7861 acagggcatc ttccttgaac ttgacgaccg ttcaaaaaca tttttgcctg tgggtcataa
     7921 aattgttttg agtcaatagg cttaagtcat ccatttgcat aaaggctctc caaaacttcg
     7981 ttagtaagct tttgatgatc ttcgccacaa agtccaatgc cgcttccttc aaaaattgaa
     8041 aggtcaacat catatttttt tagagttgcg gcttgtgctt cgtgattttt ctgaacgaat
     8101 tcttcgatgc tgccatcaaa ttctccggct tctttcaact ttcgatagcc ttcatcaatc
     8161 ggagaaccat aacaatcagt tccggaaatg aacaaaacgt tgtctggccc aatcctgtct
     8221 tttagaaatc ttgcgaagca atctgcggga acaaaaacac ctccaacatg cccaaaatgc
     8281 aaggccttat ttccgtaagg cattcctgct gtaacaactg ctcgctttgg tcatttgatt
     8341 ttgtttttat taatcataac ctctattata ctataacttt attttaactc taccccgggg
     8401 ccactactaa cgattttctt cgaaaatcga gtattg
//
LOCUS       DBNCDC010000046         8318 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_4013_length_8318_cov_7.204284, whole genome shotgun sequence.
ACCESSION   DBNCDC010000046 DBNCDC010000000
VERSION     DBNCDC010000046.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 8318)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8318)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8318
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4013_length_8318_cov_7.204284"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            183..2237
                     /locus_tag="AC04VQ_04410"
     CDS             183..2237
                     /locus_tag="AC04VQ_04410"
                     /inference="COORDINATES: protein motif:HMM:NF021028.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIO9553402.1"
                     /translation="MSEFKTAVEFVLGENPTPGGGTAASQTGDVIGIIAILLFALVAI
                     AALGFFVYSNKKSGIKYGNYFSNAETGVMTKSRKIAIGAFLISILALCGTLLIPNITK
                     AMASNDDADETIKVYVDPETQTLSSDQTTIVNKTDKTAYVAAVKTALTPEAEEILKDS
                     TFNLKIESQTLGTTLYDKAVPGVDDTVKSLPFASGQSAAAQLTISDLDFATAKLLIGI
                     KVFSVSFVEAQCYTVTYDGNEATAGTPPAAQAKIPGEDLTLAATSTLEKEGYTLEGWN
                     TAPDGTGDSYNLGAKYTTDADVKLYAFWKAVPVPPPSDEYQVTFTHGEGGTVDPISFN
                     AKIGTVYTYDAPTKVFTFTNQDDASDTHTITASETQGYHFKEWQLNGSAVTTGGTVSE
                     TTEFKAIFEVNTPIADYTITYKVLNTFDPHGSIALNSSNEDPVPSVSETVTATHPAVG
                     ATASNLTDQPEHDYNYFFDGWTIEDPKYSGWASNEFCLTAEAVAALGLTENTVFIAHF
                     IAPVAKYTEENSTMIFSCDEVIADGNYTFPVYNTNWDEEYIKDGDLSHLTYPAWCNYD
                     GTQKVYPRNVHFLTSFKKYKGLKSTSFWFNTSTLLRRVTPSNDNENNSGLYLDNQEVV
                     PILASSIQVSVLTNVSGLENIFTENLTQTAFMFATDNGRLNSIKHRLINETYLQSLDF
                     SS"
     gene            2292..3278
                     /locus_tag="AC04VQ_04415"
     CDS             2292..3278
                     /locus_tag="AC04VQ_04415"
                     /inference="COORDINATES: protein motif:HMM:NF024702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HIO9553403.1"
                     /translation="MFGGCSDLTKIDLPTEEDGHYFGTYANNLVETFVYCTSLQSISF
                     KGNFGKYVTDLTGIFAECTNLNNIEFGSAFGENTDVANSMFFDCYSLQTINFNSAFGN
                     EITVFTGMFNGCYNLTDINFGTGTKIGQKTSVCANMFAECENLRTIDLSSFVVGEDTQ
                     ASFMFYGCNSLEEFKISDKWNKNMGTTSLPNAREGWIQNATDTSEGTSFNPRQIPDPS
                     GPGTFSMRSAKAIYDPNNSTFTFYYDTKPHMSEIEAGCTIYPVSSNCGFVTGQYGNNR
                     GRVVPGREELSLLSDNNSAFDVLLESTPLNSISAQGKSRINSYILTDLRLPS"
     gene            3411..3545
                     /locus_tag="AC04VQ_04420"
     CDS             3411..3545
                     /locus_tag="AC04VQ_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553404.1"
                     /translation="MADSDGYAPFSFSQGFGNIPTANLESSACMFGGFSFEMFKEFDY
                     "
     gene            3609..4001
                     /locus_tag="AC04VQ_04425"
     CDS             3609..4001
                     /locus_tag="AC04VQ_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553405.1"
                     /translation="MDTFNNTLTTLDLSSFNISALNSRVDYGALCANCKALTNVKLPS
                     MLCPQQETNEDEDQVEVQSVSALSSGSSILSFAGLFYGCESLTSIENIEFFNPFNLLS
                     TNFSYYSLGKMFYGCRNLSKIDFSKFNT"
     gene            4764..5516
                     /locus_tag="AC04VQ_04430"
     CDS             4764..5516
                     /locus_tag="AC04VQ_04430"
                     /inference="COORDINATES: protein motif:HMM:NF015347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HIO9553406.1"
                     /translation="MTLTDAVNYANSSNYDPTILHPVKKLVTQYSNLWKIQTLIEPKD
                     VIFAENFKNYKELSSISMWFMADQFGTQTVQNFYSPVSGNDKKPGFANLYTKNLNNVQ
                     YAFGGLFEEDNNGAIIGKLVSPLDLALSFAADEATEWSKNTETYGTSLESLDLTDFDL
                     SHVPYLNAMFANCVGLKEIKFSDNLNCDNFYDCSALFANCENLGPKFSDSVINWSKFK
                     TDKVTNFDYMFYGCSGIEDLDLSNLNTTNAYV"
     gene            5532..6332
                     /locus_tag="AC04VQ_04435"
     CDS             5532..6332
                     /locus_tag="AC04VQ_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553407.1"
                     /translation="MLLPNQSNSHDANYAAGYKIFSECTRLSKITINSKYDTTKPINV
                     DGWNLNLGKSGLADCTDGVWEHTDSVNPWRPTEQSTMIPYSYSIVVDNPGVSPSDVFS
                     GTFTRSNKLVEKVVTVNSNDDNLGTVSDTKYGYINSVQIKADNVNEKAIYLDNRKITA
                     TPNEENARFAYWSIKINDDKEFAVPSNYDSTFYAENDDVISVTAVFVENEYNAYVAFS
                     ADGSGEGTFTFYYGEGTNEAASEVVPSGEELIKVFDVKPNTSDYDLPS"
     gene            6426..7541
                     /locus_tag="AC04VQ_04440"
     CDS             6426..7541
                     /locus_tag="AC04VQ_04440"
                     /inference="COORDINATES: protein motif:HMM:NF015347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HIO9553408.1"
                     /translation="MYVNFNESFAQYKELRSMSCWFMGALSHLDESVLFNPFCLDSAE
                     HFSGLNNIDTSNISNVAYMFGGHEYFNELHGSKNTKITLVGFDTEGKISNLSGLFANL
                     PLLTELNVDSACFCTMKTVTSTSKMFYNTPKLDKSTLTWLNTYGVTEMDYMFGNTVDS
                     IPQEGIVDKTSVVFPKGFTISENVKSLEGFFSGYANCKELELDFSKCEQKPSTAAVTN
                     MSYLFQNCCWVSKFTFVQADGWQTSKVTNMSHMYENCTKIKNATTGPDSSLPKCLQQI
                     MLVTDFSYMFAGCTGLEEYSINKDSYFTQVVIQSASHMFANCTSLKTVVFPGSYYWYP
                     NELSVFETVNYPAIDMFTNIDGTRNTTLEHFAFDSET"
     gene            7563..8180
                     /locus_tag="AC04VQ_04445"
     CDS             7563..8180
                     /locus_tag="AC04VQ_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553409.1"
                     /translation="MAGLADTKNNIPIGWINSDTLSYENTRRLPSVDTPRVSNYELTN
                     YDRFTTTYYAQTSDLQAWFDTAIWEEKTPISFYSNPFIGTADGYSISVELSPVSKDPD
                     FYQEIYFTITNNNSGAFKTYALIGTAPSEMTSVAWYYKAISSGATGQINPTGTTLPNE
                     DTIIYFPYLPGSEPTYSALEVANAALRIFALPTPLLIVDLLFAMF"
BASE COUNT         2644 a         1418 c         1566 g         2690 t
ORIGIN      
        1 acgagtaaat caagtactgg ccccggggaa atcgagtaaa tgccacgagt aaatcaagta
       61 ctggccccgg ggaaatcgag ttggttgact ggtgtggggt tttaggttat aatgtttcac
      121 aaataagggg gttccataat ggggccctta atttgtcgtt gtcttttgat aaggagagtg
      181 ttatgagtga atttaagacg gccgtagaat ttgttttagg cgaaaaccca acacctggtg
      241 gaggcactgc tgcttcgcaa acaggagacg taattggcat cattgcaatt ttgttgtttg
      301 ccttggtagc cattgcagcg ctgggatttt tcgtttattc aaacaagaaa tctggaatta
      361 agtacggcaa ttatttttcg aacgccgaaa caggcgttat gacgaagtca agaaagattg
      421 ccatcggtgc atttttaatt tcgatacttg cattatgtgg cacacttttg attccaaaca
      481 ttacaaaagc aatggcttca aatgacgatg cggacgaaac aatcaaggtt tatgttgatc
      541 ccgaaacgca aacattgtct tcggatcaaa ccacgattgt caacaagact gacaaaactg
      601 cttatgtagc agcagttaag accgcactca cgccagaagc cgaagaaatc ttgaaagatt
      661 caaccttcaa cctaaagatt gaatcacaaa ctcttggcac tactctatat gataaggctg
      721 tgcccggagt cgacgacact gtaaaaagtc ttccttttgc atcaggtcaa agcgctgcag
      781 ctcagctaac aatttcagat ttggattttg caacagcaaa gcttttaatt ggcataaagg
      841 ttttcagcgt ttcgtttgtc gaagctcaat gctacacagt aacttacgat ggcaacgaag
      901 ctacagccgg aactcctcct gctgcacaag caaaaattcc aggcgaagac ttgacccttg
      961 ctgcaacttc aaccctcgaa aaagaaggct acactcttga gggatggaat accgcacctg
     1021 acggaactgg cgattcgtat aatttaggtg ctaaatacac aaccgatgca gatgtaaaat
     1081 tgtatgcatt ctggaaagcg gtacctgtac ctcctccatc tgatgaatat caagttacat
     1141 ttactcatgg cgaaggcgga actgtagacc ctatttcgtt taatgcaaaa attggaacgg
     1201 tttacaccta tgatgctcca acaaaagtat ttacatttac aaatcaagac gacgcaagtg
     1261 acactcacac aataactgct tcagagactc aaggttatca ttttaaagaa tggcagctta
     1321 acggttctgc tgtaactaca ggtggaacag ttagtgaaac aactgaattt aaggctattt
     1381 ttgaagtaaa cacaccaata gctgattaca caattactta taaagtatta aatacatttg
     1441 atcctcatgg ttctattgca ctaaattcat ctaacgaaga tccagtacct tctgtatctg
     1501 aaactgttac cgcaactcat ccagctgtgg gcgccacggc ttcgaacctt acagaccagc
     1561 ctgaacatga ttacaattat ttcttcgatg gctggacaat cgaagatcca aaatacagtg
     1621 gttgggcaag taacgaattt tgcttaactg ccgaagcagt tgccgcactt ggccttacag
     1681 aaaataccgt atttattgct cactttattg caccagttgc aaagtacact gaagagaatt
     1741 ctacaatgat tttctcatgt gacgaagtta ttgcagacgg caactacaca ttccccgttt
     1801 ataatacaaa ttgggatgaa gagtatatta aggatggaga cttaagtcat ttaacttacc
     1861 cagcgtggtg taattatgat ggcacacaaa aggtataccc aagaaatgta catttcttaa
     1921 cttcatttaa aaagtataaa ggtctaaaat caacttcgtt ttggtttaat acttcaaccc
     1981 tattgcgcag agtaactcct tcaaatgata atgaaaacaa ttcaggttta tatttagata
     2041 accaagaagt tgtgccaatt ttagcaagct ccatccaagt aagtgtatta acaaatgtaa
     2101 gtggtttaga aaatatattt acagaaaatt taactcaaac agcttttatg tttgcaacag
     2161 ataatggcag acttaactca attaaacatc gcttaattaa tgaaacgtat ttgcagtcct
     2221 tagacttttc gtcatgaaat attaatccta aagacgaaaa tggtaatcaa gcttatgtaa
     2281 acatgaggtc tatgtttggt ggttgtagcg atttaacaaa gattgacctt ccaacggaag
     2341 aagacggaca ttattttgga acatatgcaa ataatttggt cgaaacattt gtttattgca
     2401 cgtctcttca gtctatttca tttaagggta attttggaaa gtatgttact gatttaactg
     2461 gcatatttgc tgaatgtact aacttaaaca atattgaatt tggatcagca tttggtgaaa
     2521 ataccgatgt agctaattca atgttcttcg attgttattc gttacaaact attaatttta
     2581 attctgcatt tggtaatgag ataacagttt ttacaggtat gtttaatggt tgttataacc
     2641 ttaccgatat taacttcggt acaggaacaa aaattggaca aaaaacttca gtttgtgcca
     2701 atatgtttgc tgaatgtgaa aatcttagaa caatagattt aagtagtttt gttgttggtg
     2761 aagatactca agcttcattc atgttttatg gctgtaattc attagaagaa tttaagattt
     2821 cagacaaatg gaataaaaac atgggtacta cttcgttgcc taatgcaaga gaaggatgga
     2881 tccaaaatgc aactgacact tccgaaggta catcatttaa tccacgtcaa attcctgatc
     2941 catctggtcc gggaaccttt tctatgagaa gtgcaaaagc aatttatgat cctaacaatt
     3001 caacatttac attttattat gacaccaagc ctcatatgtc agaaatcgag gctggttgca
     3061 caatttatcc tgtatcttca aattgcggat ttgtaactgg acaatatggt aataacagag
     3121 gaagagtagt tcctggaaga gaagagttgt ctttactctc tgataataat tctgcttttg
     3181 atgttttgtt agagtcaact ccgcttaatt caattagtgc gcaaggtaaa agtagaatta
     3241 attcatatat ccttaccgat cttcgcttgc cttcttgata cagaattgac tatgcaaatt
     3301 acagaataga gaatttgctt aagacgccaa gccaaaacag ttctattgat gttatatttg
     3361 acccaacttt tgggagattt acatcgcttt attctacttc ttgctggttt atggccgatt
     3421 cggatggcta tgctccattt agttttagcc aaggttttgg aaatattcca acagctaatt
     3481 tagaatcttc tgcctgcatg tttggtggtt ttagttttga gatgttcaaa gagtttgatt
     3541 attgaacatc tggagtagtc aataatgtaa attatgattt aggagtagga ccttatgtta
     3601 ctccgtattt ggatacattt aataacacat tgaccactct tgatttgtca tcgtttaata
     3661 tatcagcatt aaactctcgt gttgattatg gtgcactatg cgctaattgt aaggcattaa
     3721 caaatgtgaa acttccttct atgctttgtc cgcagcaaga aacaaacgaa gatgaagacc
     3781 aagttgaagt acagtcagtt tctgcattaa gttcaggatc atcgattttg tctttcgcag
     3841 gattatttta tggttgtgaa agtttgacaa gtattgaaaa cattgaattc tttaatccat
     3901 ttaatctttt aagtacaaat ttttcatatt attcgttggg caaaatgttt tatggttgcc
     3961 gaaatttatc aaaaatagat ttcagcaaat tcaatacttg acctatttgt aattcaagtt
     4021 catcggacta ttcatatata tttaaagaat gtgataagct taaagaaata acaattagtg
     4081 aacaatgata tattccaatg aattcatatt gcgcctctct gccgagcctt tcgtattatg
     4141 acataaattg aactaaagac ggcattggat acgatgttga ccaaattcca tataatgagg
     4201 ttgattgacc tgctgaacca gacaatactg gaaaatttac aagagataaa gaagatatag
     4261 aaaggcttaa acaaactgtt tctattaaat ctgaagattc agataaaggt tatgtcacct
     4321 ctaaaactta tactttctat ccaagcgatg gagttacaat aagtgtatct tctaatacta
     4381 ttagaattaa cgatagcgat atatttgcaa ttcctaaccc aggttataga tttgcatatt
     4441 gagaagttga tggttggctt tttgaagaaa atataaggac atactgaaat tctatagaaa
     4501 ttgttgctca ttttgaacct gttgattatt cggcaaaagc tgtttatacc aaaaataaca
     4561 ccaagtatga tggcgataac actctaactt tctattatga caacgttgac cacagtcttg
     4621 aaggcgaagt atacgaagtt tcgccaaata cttttgctga tactggtaat aattatgctt
     4681 tgttgccagc ttgatttaaa actacatatg ttgcaaaagc cgattctgga cagggctgaa
     4741 aagaacctga aattgcgaaa agattgactt taacggatgc agtaaattat gctaattcaa
     4801 gtaactatga tccaacaatt ttacatccag ttaaaaaatt agtaacacaa tacagtaatt
     4861 tatggaaaat acaaacgctt attgaaccaa aagatgtaat ttttgctgaa aactttaaaa
     4921 actacaaaga gctttcgagt atttcgatgt ggtttatggc ggatcagttt ggcacacaaa
     4981 ctgttcaaaa tttctatagt cctgtttctg gaaacgataa aaaacctggt tttgctaatt
     5041 tgtataccaa gaatttgaat aatgtccaat atgcttttgg tggtttattt gaagaagata
     5101 ataacggtgc aattatagga aaattagtct cgccgcttga ccttgcatta tcttttgctg
     5161 ctgatgaagc tactgaatgg tctaagaaca ctgaaactta tggtacatct ttagaaagct
     5221 tagatttgac agatttcgat ctttctcatg ttccttattt aaatgctatg tttgcaaatt
     5281 gtgttggact taaagaaatt aagtttagcg ataatttaaa ttgtgataat ttctatgatt
     5341 gcagcgcgtt gtttgcaaat tgcgaaaacc ttggaccaaa attcagcgat tctgttatta
     5401 actggtctaa gtttaagact gataaagtta cgaattttga ttatatgttc tatggatgta
     5461 gcggtatcga agatcttgac ctatctaatt taaacactac taatgcttat gtttgaaatt
     5521 ggagatcagc tgtgctatta ccgaatcaat caaactctca tgatgcgaat tatgctgctg
     5581 gatataagat atttagtgaa tgtacaagac tttcaaaaat cacgattaat agtaaatatg
     5641 acacaactaa accaatcaat gttgatggtt ggaatttaaa tcttggaaag tctggccttg
     5701 cagattgcac agatggtgta tgggaacata cagattcggt aaatccatgg cgaccaactg
     5761 aacaatctac aatgattcct tattcttatt cgattgttgt tgacaatcct ggcgtgtctc
     5821 cgtctgatgt attttctggc actttcacaa ggtctaataa acttgtagaa aaagttgtaa
     5881 ccgttaattc taacgatgat aatttaggaa ctgttagcga tacaaaatat ggatatatca
     5941 atagtgttca aattaaggca gataatgtaa atgaaaaagc aatttatttg gacaatagaa
     6001 aaataactgc tactcctaat gaagaaaatg caaggtttgc ttattggagc atcaagatta
     6061 atgatgacaa agaatttgca gtgccttcga attatgactc aactttctac gcagaaaatg
     6121 atgatgtaat tagtgtaact gccgtatttg ttgaaaatga atataatgct tatgttgcgt
     6181 tttctgcgga tggttctggc gaaggaacct ttacattcta ttatggcgaa ggcacaaacg
     6241 aagccgcttc ggaagttgtt ccaagtggcg aagagctaat taaggtcttc gatgttaagc
     6301 caaatacaag tgattacgac ttgccttcgt gatatagaaa atatcaagag tcctggtatc
     6361 agggtgataa tcatcctgtt ccaaagtttg ttcgcgaagt tgaagataat gtagacaaca
     6421 acaaattgta tgttaatttt aatgaatctt ttgctcagta taaagagctt cgaagcatgt
     6481 cttgttggtt tatgggtgcg ctttcgcatt tagacgagtc tgttctgttt aatccattct
     6541 gtttggattc ggctgaacac tttagcggcc ttaacaacat tgatacttca aacatttcaa
     6601 atgttgctta tatgtttggt ggtcatgaat atttcaatga gcttcacggt tctaagaata
     6661 caaagattac attggtcggc tttgatactg aaggtaaaat tagtaatctt agtggattgt
     6721 ttgctaactt gcctttgtta acagaactta atgttgatag tgcttgcttc tgcactatga
     6781 agacggttac ctcaacttca aagatgttct ataatacgcc aaaacttgat aagtctacac
     6841 ttacatggct taatacttat ggtgtaactg aaatggatta catgtttggc aatactgtag
     6901 actctatacc tcaagaggga attgttgata aaacaagtgt tgtattccct aaaggattca
     6961 caatatcaga aaatgttaag agcttggaag gtttctttag tggttatgca aattgcaaag
     7021 aactagagct tgatttttct aagtgtgaac agaagccatc aactgctgct gtaaccaata
     7081 tgtcgtactt attccaaaat tgttgctggg tttcaaagtt cacatttgtt caagctgatg
     7141 gttggcaaac ttcaaaggtt accaatatgt cacacatgta tgaaaattgc accaaaatca
     7201 aaaatgcaac gactggtcct gattcttcgc tgccaaaatg cttacagcaa attatgttag
     7261 ttactgattt tagttatatg tttgctggtt gtactggttt agaggaatat tcgattaata
     7321 aagattcata ttttacacag gttgttattc aatctgcaag tcatatgttt gctaattgca
     7381 caagcttaaa gaccgttgta tttcctggta gttattattg gtatccgaat gagctgtcag
     7441 tatttgaaac agttaattat cctgctatag atatgtttac aaatattgat ggaactcgca
     7501 atactacatt agagcatttt gcatttgata gtgagacttg agattattat gtttcattct
     7561 atatggctgg tcttgcagat actaagaata atattcctat aggctggatt aacagtgata
     7621 ctttatccta tgaaaataca cgaaggctcc catctgttga cactccaaga gtttctaatt
     7681 acgaattaac caattatgat cgctttacta ccacatatta tgctcaaact agcgatttgc
     7741 aagcgtggtt tgatactgct atttgggaag agaaaactcc aataagtttc tatagtaatc
     7801 cttttattgg aactgcagat ggatatagta tttctgtaga gctatctcct gtttcaaaag
     7861 atcctgattt ttatcaggaa atttatttta ctatcactaa taacaattca ggcgcattca
     7921 aaacttatgc acttattggt actgctcctt ctgagatgac ttcagttgct tggtattaca
     7981 aggcaattag ttctggagct actggtcaaa taaatcctac tggtaccact ttgcctaatg
     8041 aagatactat aatttatttc ccatatcttc caggttcaga acctacatat agcgcgttgg
     8101 aggttgcaaa tgcggcatta agaatcttcg cattgccaac tccattatta atagtagact
     8161 tgctcttcgc tatgttctaa gtttaagtgg cataaggtaa acaactgtta aggcctcacg
     8221 aaagtggggc cttttcctta ctcgatttcc ccggggccag tactcgattt actcgtggca
     8281 tttactcgat ttccccgggg tcagtactcg atttactc
//
LOCUS       DBNCDC010000047         8117 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_4201_length_8117_cov_7.145125, whole genome shotgun sequence.
ACCESSION   DBNCDC010000047 DBNCDC010000000
VERSION     DBNCDC010000047.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 8117)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8117)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8117
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4201_length_8117_cov_7.145125"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(143..1471)
                     /locus_tag="AC04VQ_04450"
     CDS             complement(143..1471)
                     /locus_tag="AC04VQ_04450"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIO9553410.1"
                     /translation="MVKVKGDVMEENKSAFAGGTKVTFNTGGTYSSGPKKKPNIVEKA
                     TQKVTKGDPNKKMFLYGFLGALLACIIVLLIFSLANCGKKVTLGATDSVTIENADESE
                     NLAEAVANKCLPSVVAINVYTTTRGTESLTSLGSGVILSADGYVITNQHVIEGATKIK
                     VTANGQEYEADKVGEDSSSDIAVIKLKNANDLQAIELADSDQVKVGAWVMSIGSPFGL
                     EQSVATGIVSATSRSQVVSSQTDGSNKVYPNLIQTDAAINPGNSGGALVDKNGRLLGI
                     NALIESDSGNYSGVGFAIPVNYAVGLAKDIIDGKTPSHAQLGISMQNVTASNSKQYGW
                     AVSEGAYVSGVTSGGSAEKAGIKVGDIITKIENDKISTTSDALLAVRKHNPGDTISIT
                     FNRAGEEMTVQATLGSDEGQTQQKQQQQQQQNNNKNYNDMYDLWKYFFGF"
     gene            1642..2367
                     /locus_tag="AC04VQ_04455"
     CDS             1642..2367
                     /locus_tag="AC04VQ_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HIO9553411.1"
                     /translation="MTVMIAALIALGIIGFGYLEGCMMYKDISDDAFDDGNLNLADMK
                     VDWDALLKINPETVAWVYIPGTPVNYPVVHTTDNEKYLHTNFRGYTSYVSYGAIFMDC
                     NNKHDLSDQNIVTYGHNMNDGSMYAVFRQMKDSNVFNQNRNIYFLTPKGNYRLKSFSL
                     CHVNAEEKIIQTNFGTEANKVKYIMDKINRCIVDVDGQIPDPAAMTKIFTFSTCDNTD
                     RNYRDMCFAYVAESTVDGVPGLE"
     gene            2375..2839
                     /locus_tag="AC04VQ_04460"
     CDS             2375..2839
                     /locus_tag="AC04VQ_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553412.1"
                     /translation="MKFLKNNIQKIFIGVIIIIVLGVVLLRGDSLKELIDTMQGATPI
                     PIILAVLTQLAKYFAQSFAYSFSFKAVGEKMRARETLPLVFGTFFMNTIAPSMNLAGT
                     TLVVDDARRRGIDPGKSTSAALLMQITIDGAFTTVMIGAFILLLCTVGLNPL"
     gene            2846..3064
                     /locus_tag="AC04VQ_04465"
     CDS             2846..3064
                     /locus_tag="AC04VQ_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553413.1"
                     /translation="MGSVVVVLVLVMILLLALGKINPKLLHFMLGKVEALINRLSLKV
                     RKKPVKPWAERIVDNFSDASHLVSQNMR"
     gene            3068..3538
                     /locus_tag="AC04VQ_04470"
     CDS             3068..3538
                     /locus_tag="AC04VQ_04470"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIO9553414.1"
                     /translation="MAAYGCSLFASVCELSCFALCGIGFEILAPEPLICGYVVATLFA
                     MISITPQGVGVVEAMVVVAFTAYDQNAAAATATALVYRGIVFWMPFIIGAILMTQIKA
                     FKDSSKKSVEGVGKIAEEYNEKGELNLREKVQQAHEEFEKEKEQNEVHLENSDT"
     gene            3540..5501
                     /gene="abc-f"
                     /locus_tag="AC04VQ_04475"
     CDS             3540..5501
                     /gene="abc-f"
                     /locus_tag="AC04VQ_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HIO9553415.1"
                     /translation="MLLQLENIEKTYPGKTLFSNVNLFLEAGDRMALVGANGAGKTTL
                     LNIICGEEIADKGSVTIAKDVNVGYLTQEAIEMNDVEIFDEVISAQKDILELEKKLEN
                     LEAQIAEDPDSADMDLYGKLQDKFESLNGYALKAKVQSVMFGLGFKESDLRRKTSEFS
                     GGWQMRIALAKLLVQSPEILLLDEPTNHLDLDSVKWLEQFLNDYPGTIVLVSHDREFM
                     DNMVTKVVEFANGEIKQYRGNYSKYEKEREAYIEILKAKRSKQLDEMHRLEVFVERFR
                     YKATKARQAQERLKRLEKLRQELVEIPEATKKVHFHFEQPPRTGDLVVHANSVSKAYG
                     DNIVYDKADFKMYRGDKIALVGPNGAGKSTLLKMVAGDLQPDSGRVKYGVHVDLMYYA
                     QHQLDKLNTENTVYTEVEKIAPGWSMGQIRNLLGAFLFKGDDVEKLVSVLSGGEKSRL
                     ALAKMLVEPAPLLCLDEPTNHLDISSCDVLEQALRTFEGTLLFITHDRHLINSVANKI
                     VEIMPGKITMFDGNYDYYLYKKQILQESKDEDRMLFDKTTLKSPEYVGATPRVRTVKD
                     TKPQSGPKTKEQKRVEAERRNAIYAATHQLKDRLGEVNQELAENNAKMKELLEVLADP
                     EFYTNRDDAQDVIMEHAELKQKIKELEDE"
     gene            5543..5938
                     /locus_tag="AC04VQ_04480"
     CDS             5543..5938
                     /locus_tag="AC04VQ_04480"
                     /inference="COORDINATES: protein motif:HMM:NF018249.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553416.1"
                     /translation="MKTIILVPYVASGESNEVPAAEAKFLHEEHFKDASYKQILGTGL
                     EGRLYIKGDVGLYIIGEGKTNASMFTTALLNDKEIVEPDTKFILFGCCGCTKEVGVVG
                     DIYFVAETVDGELGHHADAREDGSSSDHS"
     gene            6011..6145
                     /locus_tag="AC04VQ_04485"
     CDS             6011..6145
                     /locus_tag="AC04VQ_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553417.1"
                     /translation="MVKDEKAESSEKAKSFMAKSFNNEAWATRDPKIMKATSVTADSY
                     "
     gene            6224..6439
                     /locus_tag="AC04VQ_04490"
     CDS             6224..6439
                     /locus_tag="AC04VQ_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553418.1"
                     /translation="MEDIAVAQVFKNYNLLDQLLVMRFAVNTDVFMADQNPENLWGEG
                     KGSFDSSAFDNFDDAFPVVTKRATEIL"
     gene            complement(6570..7121)
                     /locus_tag="AC04VQ_04495"
     CDS             complement(6570..7121)
                     /locus_tag="AC04VQ_04495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9553419.1"
                     /translation="MVLLTSSLMMVVITKSAGNENLNKRLKENKNSKGTYASKTFHNF
                     ALFLISFIAISIVQAGILDYTPTILQSRFDMSGSLSGILTSLTMVISIGSSLLFGAFC
                     DKFKTYKYVYVIGCALMGPGACLMFTCSGPLFVLGQILLGIGLGAPAVSANAVFEILE
                     PKYHSLGIGIGMAAFSLGQVLGS"
     gene            complement(7137..7502)
                     /locus_tag="AC04VQ_04500"
     CDS             complement(7137..7502)
                     /locus_tag="AC04VQ_04500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9553420.1"
                     /translation="MTSYSMVATILAIPTGWFSRKLGYKSLLIIGIALSIIGSLIGSV
                     AFNLPTGNIQVLIVGRILEGTCYIFSSVCIPIIIQRIIKDHHIGKVMGIWAIRVPIAC
                     FIGESLTPIFEESFGLPTT"
     gene            7960..>8117
                     /locus_tag="AC04VQ_04505"
     CDS             7960..>8117
                     /locus_tag="AC04VQ_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIO9553421.1"
                     /translation="MAKILGIDLGTTNSAMAVMEGTEPEILVNSEGDRTTPSVEGFRK
                     DGERVVGKA"
BASE COUNT         2497 a         1415 c         1685 g         2520 t
ORIGIN      
        1 ttcgaacccc agataccctt ttggggcata cacgatttcc aatcgtgctc cttcgaccag
       61 ctcggacatc tctccaaaaa ataagcctgc gccaaaattc gacgcaggct cgtattatag
      121 ccgaatctaa attatttact aattaaaatc cgaaaaagta tttccataaa tcatacatgt
      181 cattgtagtt tttgttgttg ttttgttgtt gttgctgttg ttgtttttgt tgagtttgac
      241 cttcgtcaga tccaagagtt gcttgaacag tcatttcttc gcctgctctg ttgaaagtta
      301 tgctaatagt gtctcctgga ttatgtttac gaacagctaa aagagcatcg cttgttgttg
      361 aaattttgtc attttcaatt tttgtaataa tgtctccaac tttaattcca gctttttcag
      421 ctgagccacc gcttgtgact ccagaaacat aagcgccttc gctaactgcc catccatatt
      481 gttttgaatt tgaagcagta acattttgca tagaaattcc aagttgagca tgtgatggag
      541 tttttccatc gatgatgtct ttcgctaaac caacagcgta atttacagga atagcgaagc
      601 caacgccaga ataattacca gaatcacttt cgattagagc attaatacct aacaaccttc
      661 catttttatc tactaatgca ccaccactgt ttccagggtt gattgcagca tctgtttgaa
      721 tgaggttagg gtagaccttg ttcgatccat cagtttgaga tgatacaact tggcttcttg
      781 atgttgccga aacaattcca gttgctacgg attgctcaag accaaatgga cttcctatac
      841 tcattaccca tgcgccaact ttaacttggt cgctgtctgc aagttcgatt gcttgaagat
      901 catttgcgtt ctttagcttt attacagcaa tgtcagaact tgagtcttcg ccaactttgt
      961 cagcttcgta ttcttggcca tttgcagtta ctttaatttt agttgctcct tcaattacgt
     1021 gttgattagt tataacgtat ccatcagcac ttaaaattac gccgctaccc agcgaagtta
     1081 aggactccgt tcctcgggtt gttgtgtaaa cattaattgc aacaacactt ggcaagcact
     1141 tgttggcaac tgcttcggca aggttttctg attcatcagc attttcaatt gttacactat
     1201 cagttgctcc caatgtaact ttctttccac aatttgccaa tgaaaaaata agtagtacta
     1261 ttatgcacgc aagtagggcg cccaaaaaac catataaaaa catctttttg tttgggtctc
     1321 cttttgtaac tttctgggtg gctttttcaa caatattagg tttctttttt ggaccactgc
     1381 tataagtgcc tccagtgtta aaagttacct ttgttccacc tgcaaaagct gatttatttt
     1441 cttccataac atctccttta acttttacca ttttagcatt ttaatcttat agattaaata
     1501 aaagttaaac aaacattagg taggttgatg ttgttctgtg tggtataatg gctggctatg
     1561 gttaataacg aagaaataac agccagtcat gcaagctcag caaaagttaa gaaaaaacgc
     1621 ggatttttgt tttgagcttc gttgactgta atgattgcag ctttaattgc tctcggaatc
     1681 attggatttg gatatcttga gggctgcatg atgtataaag acatttctga tgatgctttt
     1741 gatgatggaa atttgaacct cgcagatatg aaagttgatt gggatgctct tttaaagatc
     1801 aatcctgaaa ctgttgcttg ggtctatatt cctggcactc cagttaatta tcctgttgtt
     1861 cacacgactg acaatgaaaa atacttgcac acgaatttta gaggatatac ttcatatgta
     1921 tcttatggag caatatttat ggattgcaat aacaagcatg atttaagcga tcaaaacatt
     1981 gtcacttatg gacacaacat gaacgatggt tcaatgtatg cagtttttag gcagatgaaa
     2041 gattcaaatg ttttcaacca aaacaggaat atttattttc taactcctaa aggaaattat
     2101 agattgaagt ctttttctct ttgtcatgtt aatgcagaag aaaagattat tcaaacaaat
     2161 tttggcacag aagctaacaa ggtaaaatat attatggata aaattaatcg atgcatagtt
     2221 gatgttgacg gacaaattcc agatccagca gctatgacaa agatttttac tttttcaaca
     2281 tgcgacaaca cagatagaaa ttaccgtgat atgtgttttg cctatgttgc cgaatcgacg
     2341 gtagatggag ttcctggctt agaataaatt tgttatgaaa ttcctaaaga acaatattca
     2401 aaagatattt attggggtaa ttattattat tgttttgggc gttgttcttc ttcgtggaga
     2461 ttctctaaaa gaattaatcg acacaatgca aggcgcaaca ccaatcccaa ttattttggc
     2521 ggtcttaact cagctggcga agtacttcgc tcaaagtttt gcctattctt ttagctttaa
     2581 agctgttggt gaaaagatgc gagcaagaga aacgcttcca cttgtttttg gaaccttctt
     2641 catgaatacc attgcacctt cgatgaattt agctggcaca acattggttg ttgacgatgc
     2701 aagacgaaga ggtatcgacc ctggaaagtc tacgtcggct gcattgctta tgcaaattac
     2761 aatagatggc gcatttacga ctgttatgat tggcgctttc attttgcttc tttgcacggt
     2821 tggtcttaat ccgctttgaa ttttaatggg aagcgttgtt gttgttttgg ttttggttat
     2881 gattttgctt cttgctcttg gcaaaattaa tccaaagctt ttgcatttta tgcttggaaa
     2941 ggtagaggcc ttaatcaata ggctttcgct aaaagttcgc aaaaaacctg ttaagccttg
     3001 ggctgagcgc attgttgata atttttctga tgcttcacat ttagtaagtc aaaatatgag
     3061 atgagttttg gctgcttatg gatgcagttt gtttgcaagt gtttgcgaac ttagctgctt
     3121 cgctttatgc ggaattggtt ttgaaatcct tgcgcccgag ccattaattt gtggttatgt
     3181 tgttgccacg ctctttgcca tgatttcaat tacgcctcaa ggtgttggtg ttgttgaggc
     3241 tatggttgtt gttgccttta ctgcttatga tcaaaacgct gcagccgcaa ctgctactgc
     3301 tcttgtttat agaggtattg ttttctggat gccatttatt attggtgcaa ttcttatgac
     3361 tcaaattaaa gcttttaagg attcatcaaa gaaatctgtc gaaggggttg gtaaaattgc
     3421 tgaagaatat aacgaaaaag gcgagctaaa tcttcgtgag aaagttcaac aagcccatga
     3481 agaattcgaa aaagaaaaag agcaaaacga agttcatcta gaaaattccg acacctaaaa
     3541 tgcttctaca gctagagaat attgaaaaaa cttaccctgg taaaacttta ttttcaaatg
     3601 tcaatttatt tttagaagca ggagaccgta tggcgcttgt tggtgctaac ggtgcaggta
     3661 aaaccacgct tttaaatata atttgtggcg aagaaatagc cgataaggga agtgtgacaa
     3721 ttgctaaaga tgtcaacgtt ggctatttaa cgcaagaagc aatagagatg aacgatgttg
     3781 aaattttcga cgaagtaatc tccgctcaaa aagacatttt agaacttgaa aagaagttgg
     3841 agaatcttga agctcaaatt gccgaagacc cagactctgc cgacatggat ttgtatggca
     3901 aacttcaaga taagttcgaa tcgctaaacg gatacgctct taaagcaaaa gttcaaagcg
     3961 ttatgtttgg tcttggtttt aaagagagtg atttgcgccg gaagacttcg gagttttctg
     4021 gcggatggca aatgagaatt gcgcttgcga agttgcttgt tcaaagtcca gaaattttac
     4081 ttttggatga gccaaccaat catcttgatt tggatagcgt taaatggctc gagcaatttt
     4141 taaatgatta cccaggcact attgtgcttg tatctcatga cagagaattt atggataata
     4201 tggttacaaa agttgttgag tttgctaatg gtgagattaa gcaatacaga ggcaactatt
     4261 ctaaatacga aaaagagcgc gaggcctata ttgaaatctt gaaagcaaag aggtccaagc
     4321 agcttgatga gatgcatcgt ttggaagttt ttgttgagcg ttttagatat aaagcaacga
     4381 aggcaagaca ggcgcaagaa cgtttgaagc gacttgagaa gcttcgtcaa gaattggttg
     4441 aaatccccga agccacaaag aaggtccact ttcacttcga gcaacctccg cgcactggag
     4501 atttggttgt gcatgcaaat tcggtttcga aagcctatgg cgataacatc gtttacgata
     4561 aggccgattt taaaatgtat aggggagata agattgctct tgttggccct aatggtgcag
     4621 gaaaatcgac acttctaaag atggttgctg gagatttgca gccagacagt ggacgcgtta
     4681 aatatggtgt tcatgttgat cttatgtatt acgctcagca ccaattggat aaattgaata
     4741 cagaaaacac ggtttataca gaggtcgaaa aaattgctcc tggatggagc atggggcaga
     4801 ttcggaattt gttgggagct tttcttttca agggcgacga tgttgaaaag cttgtgtcag
     4861 tattgtctgg tggtgaaaaa tctcgtttgg ctttggcgaa gatgttggtt gagccagcac
     4921 cacttctgtg tttggatgag cctacaaacc atcttgatat ttcaagttgc gatgtgctgg
     4981 agcaggcgct tcgcaccttc gaaggaacgc ttttgtttat cactcatgat aggcatttga
     5041 taaattctgt tgctaataaa attgtggaga ttatgcccgg caagattacg atgttcgatg
     5101 gcaactacga ttactattta tataagaagc aaattttgca agaatcaaaa gatgaagacc
     5161 gaatgctttt tgacaagaca acgttaaagt ctccagaata tgttggggcg acgcctcgcg
     5221 ttcggactgt taaagatacg aagcctcaaa gtggtcctaa aactaaggag caaaagcgtg
     5281 tcgaagctga gcgccgaaat gcaatttatg cggccactca tcagctaaaa gacaggcttg
     5341 gtgaggttaa tcaggaactt gctgaaaata atgctaaaat gaaggagctc ttagaggttt
     5401 tggcagatcc tgaattttat actaatcgtg acgatgcgca agatgtgatt atggagcacg
     5461 ctgaattaaa acaaaaaatt aaagagcttg aagatgaatg attcgagatt aatgaaaaga
     5521 tagagaaggc aggaaagctt taatgaaaac aataatttta gttccatatg ttgcctcagg
     5581 ggaatcaaac gaagttccag cggcagaagc aaaattctta catgaagagc actttaagga
     5641 cgcaagttat aagcaaattt tagggactgg tcttgagggt agactttata tcaaaggtga
     5701 tgttggactt tatataatcg gcgaaggcaa gaccaacgct tcgatgttta ctacagctct
     5761 tttaaacgat aaagaaattg tagagccaga cactaagttt attttgttcg gctgttgcgg
     5821 atgcacaaaa gaagttggag ttgttggtga catttacttc gttgctgaaa ctgttgatgg
     5881 cgaattgggt catcacgcag atgcccgcga agatggaagc agcagtgacc attcatgata
     5941 tccaaatgaa tcgtttgcgc gttttggtta tgttaatctt gaaaatgatt gttttaataa
     6001 agcttttgac ttggttaaag atgaaaaggc tgaatctagt gaaaaagcta aatcttttat
     6061 ggcaaagtct tttaacaacg aagcatgggc tacacgcgat ccaaaaatta tgaaggctac
     6121 ttcggtgact gcagactcgt attgaaaagg aaactacgat catcaaaatg ctaaatatgt
     6181 tgttgacact tataagtgca agcatccttt tgccgcaaca gacatggaag atattgctgt
     6241 tgctcaagtc tttaaaaatt acaacttact tgatcaatta ttagttatgc gttttgctgt
     6301 aaatactgat gtatttatgg ctgatcaaaa tccggaaaac ttatggggcg aaggcaaggg
     6361 aagcttcgat tcgtcagcat ttgataattt cgatgacgca tttcctgtcg tgacaaaacg
     6421 tgccactgaa attctatgaa aaattattaa cgattagctc ttgttttgct aaatagtgca
     6481 attattgtgc cgatgattcc agttaaaaga aggcaaatcg cacaaataga tacattttca
     6541 aatgagttat taagaataat ttgagtggtt caagatccta aaacttgtcc gaggctaaaa
     6601 gctgccattc caattccaat accaagggag tggtactttg gttctaaaat ttcgaataca
     6661 gcatttgccg aaacagctgg agctccaagt ccaataccaa gcaaaatttg tcctaaaaca
     6721 aataatggac cggagcatgt aaacattaag catgcaccag gacccattag agcacatcct
     6781 ataacgtata catatttata tgttttgaat ttatcgcaga aggctccaaa taaaagggag
     6841 gagccaattg aaataaccat ggtaagtgaa gttaaaatcc cagacaatga accgctcatg
     6901 tcaaaacgag attgtaaaat tgttggtgta tagtccaaaa tacctgcttg aactattgaa
     6961 atagctataa atgaaattaa gaacaatgca aaattatgaa atgttttgga cgcgtaagta
     7021 cccttgctat ttttattttc tttcaatcgt ttatttagat tttcattacc agcgctttta
     7081 gtaattacta ccatcattaa agatgatgtt aacaacacca ttatgctaaa gataattcat
     7141 gttgttggaa gaccgaaact ttcttcgaag atgggtgtta agctttcgcc tataaagcaa
     7201 gcaattggaa ctcgaattgc ccaaataccc atgacttttc ctatatgatg atctttgatt
     7261 attctttgaa ttatgattgg aatacaaaca ctagaaaaga tatagcaggt accttctaaa
     7321 attctaccaa caattagaac ttgaatattt ccagtaggca agttaaaggc aacagaacca
     7381 atcaaggagc ctattattga caatgcgatt ccaataatta acaagctttt gtagccaagt
     7441 ttcctgctga accatcctgt gggaatcgca agtattgttg ctaccataga atagcttgtc
     7501 ataagtcaag aggcgagtat aggttctatt tctaatcgtg tatagatcgt ttcatagata
     7561 gaagggattt tatacatatt aatgcaatcg caaacgccaa agagaagtat ggctgcggca
     7621 attatcaaac attttagtgc attacttttc gtcgtattct cgaacatgag tgcatatgat
     7681 aacaaattgt gagaaatttt agaatacttt tctcaacggc ttcgcggtat gccctcgtgc
     7741 gttcgccagc aatcgcatgg cgctgcaggg gtttcgaaaa tgtattccaa aatctctcac
     7801 aacctggagc ttctctaaaa acgtaaaaaa tctcaaaaaa gtttgtaaaa aaacttgaca
     7861 aagacacact aaggttttat aatacgcaca acgcagagat aaagctgcgt tatatttttg
     7921 gtaacaataa aagtgattat ataaaaggag atgattaaaa tggcgaaaat tttaggaatc
     7981 gatttgggaa ccacaaattc ggcaatggct gttatggaag gcactgagcc tgaaatttta
     8041 gttaattccg aaggcgacag aacaacacca agtgttgaag gtttccgcaa ggatggcgaa
     8101 cgtgttgtag gaaaggc
//
LOCUS       DBNCDC010000048         8081 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_4240_length_8081_cov_5.762896, whole genome shotgun sequence.
ACCESSION   DBNCDC010000048 DBNCDC010000000
VERSION     DBNCDC010000048.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8081)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8081
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4240_length_8081_cov_5.762896"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="AC04VQ_04510"
     CDS             <1..740
                     /locus_tag="AC04VQ_04510"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: protein motif:HMM:NF004817.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase subunit"
                     /protein_id="HIO9553422.1"
                     /translation="GKACANALKDAGAKVVIVDVSVETGDVIDGMNCIKCDITSRENV
                     YAMVDKVVENFDKVDILVNNAGVNFPRLLVDVKGEAPEFEVNEKDLDIMFNINVKGLF
                     WCAQACAKDMLKRKEGVIINMSSESGKEGSQGASIYAATKGAVDSATRSWAKELGKHN
                     IRVVAVAPGIIEATGLRNDAFKKALAYTRGAKDYSEVGTDYSKVIPLGRDGKLTEIAD
                     LVAYLASDRSSYVSGTTFNISGAKSRG"
     gene            733..1530
                     /locus_tag="AC04VQ_04515"
     CDS             733..1530
                     /locus_tag="AC04VQ_04515"
                     /inference="COORDINATES: protein motif:HMM:NF024230.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIO9553423.1"
                     /translation="MVNKVKHTSVAKKSDTGSKIVDLALEIAYLQLISGAEMERVDRT
                     IQKICDAYDATNIEYFTYPRFIILSLDYEGKTYTKSKKIRDKAPNFHRLEELSKVFDD
                     ILDSKPDIDIVIQRVRMLYTTKQFGVLAQMFCYFFGPAGFTIFFGGNFYEAAISGVIG
                     LLILLITFGVKKLTDNFFFPTVIYSFVMSLSVRILGALAIVQNVESMQIGLLMMVVPG
                     LIITNSMREFISGEYLTGYSKLIEALLVALAVAMGVIFAAALMRIFV"
     gene            1530..1883
                     /locus_tag="AC04VQ_04520"
     CDS             1530..1883
                     /locus_tag="AC04VQ_04520"
                     /inference="COORDINATES: protein motif:HMM:NF018448.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIO9553424.1"
                     /translation="MEEMLTTYIIPVAAIFVASIAFSIFYNVKRSSILLCAIGGALGW
                     AVYLAFVNNIGHKYVSFFIAAAAITIFAEVVGRVKNVPVTIFLTISLIPLVPGRAIFN
                     TIESLLKIDKQGFMD"
     gene            complement(2005..2631)
                     /gene="pyrE"
                     /locus_tag="AC04VQ_04525"
     CDS             complement(2005..2631)
                     /gene="pyrE"
                     /locus_tag="AC04VQ_04525"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIO9553425.1"
                     /translation="MSREETIAKDLLKIKAVFLRPDEPFTWASGIKSPIYCDNRLTLS
                     DIAVRNDIENALADIVREQYPECEMVMGTSTAGIAHAALVGNILKLPMGYVRGSSKDH
                     GRNNKIEGKCEPGTKVVVIEDLISTGGSCIDVVDSLREVGAEVLGIVSIFTYGMQKSV
                     DRLAEAKVKNVSASNVDVLVEVAAEEGYIKPEDKDRIIQFRNNPSDES"
     gene            complement(2633..3328)
                     /gene="pyrF"
                     /locus_tag="AC04VQ_04530"
     CDS             complement(2633..3328)
                     /gene="pyrF"
                     /locus_tag="AC04VQ_04530"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIO9553426.1"
                     /translation="MDKDIIIACDFSSAQETYEFLDQFAEEKPYVKIGMELYYAEGPS
                     IVREIKAREHKIFLDLKLHDIPNTVSKAASQLAKLGVDMTNVHCAGGTKMMAAAKKAL
                     DGSSTKLIAVTQLTSTSEEVMHEELLLDNSKTIKEVVINYAQNAKDAGLDGVVCSPLE
                     AGEIKKSIGDEFLTVTPGIRFADDAKGDQVRVTTPADAKVSSDFIVVGRSITAASDPV
                     AAYNRCVQDFLGE"
     gene            complement(3328..4239)
                     /locus_tag="AC04VQ_04535"
     CDS             complement(3328..4239)
                     /locus_tag="AC04VQ_04535"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HIO9553427.1"
                     /translation="MVDTSVSLSGLKLDNPIIPASGTFGFGYEFAEHYDINILGSIAI
                     KGTTKEARYGNELPRIAETPSGMLNSVGLQNPGVDEVLRTELPKLQEVFSKKILANVS
                     GFSKEEYVDVASKLDAHDSVGIVELNISCPNVHGGGAAFGTDAKLAAEITSAVKKVCK
                     KPVYVKLTPNVTDIVDIATSVADAGADGLVLINTVLGARFDIKTGRPILDNVTGGLSG
                     PAVLPIALRAVYQVAQAVKIPIVGCGGIQKAEDIIEMMSAGATAVEVGAANCADPLAC
                     KKIIEDLPKVCEKLGINSLSEIVGRSL"
     gene            complement(4233..4742)
                     /locus_tag="AC04VQ_04540"
     CDS             complement(4233..4742)
                     /locus_tag="AC04VQ_04540"
                     /inference="COORDINATES: protein motif:HMM:NF021903.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HIO9553428.1"
                     /translation="MSQQKKGANLDVLMPLGNGFNLTGEEQLVVGGGIGCPPLLGACK
                     KLNELGVRPKIILGALSKDEVILEDEFKEYGEVYISTDDGSYGEPGLVTDIIKKEGFT
                     NLPYIACGPLPMEKAIVNTMNSKGKLSLEARMGCGFGACMGCSIETKGGPKRICKDGP
                     VFESEELLW"
     gene            complement(4954..>8081)
                     /gene="carB"
                     /locus_tag="AC04VQ_04545"
     CDS             complement(4954..>8081)
                     /gene="carB"
                     /locus_tag="AC04VQ_04545"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIO9553429.1"
                     /translation="IVIGQAAEFDYAGTQACLALKEEGYSVVLVNSNPATIMTDTQIA
                     DKVYMEPLKLEYIARIIRKERPDALLPSLGGQTGLNMAMKLAEKGILDECKCEILGTS
                     FDSINNAEDRLRFKELCERIGQPCLQSEIAHTVEEAREIANEIGYPIVLRPAFTLGGT
                     GGGFVYNDQELEEIAKNALKISPVTQVLVEKSIKGFKEIEFEVMRDSNGTAIVICSME
                     NVDPVGVHTGDSMVVAPVQTLSDKEYHMLSNAALELITELDIEGGCNVQFALDPFSFE
                     YYVIEVNPRVSRSSALASKATAYPIARVSSKIAVGMHLEEIQLASTCAAFEPSVDYVV
                     CKVARFPFDKFESADHELNTQMKATGEVMSIGRNFEESLLKAIRSLEIGVNHLYLEKF
                     DKFSNKELLDFIKTPSDDRFFAIAECFRRKIDLMAINHATQIDPFFLDKIHHIVSLEK
                     KVKDAPNVKNLRKCKEFGFSNEYLAPIYKMKDKQFYDYTKRNHILPVYKQIDTCGAEF
                     IADVPYLYSTYEDENESVVSKRKKIIVLGSGPIRIGQGVEFDYSTVHAIWAIQQKGYE
                     AIVINNNPETVSTDYAVSDKLYFEPLCVEDVMHIIDLEKPDGVIVSLGGQTAVNLSTK
                     LKEYKVPIIGTDVDAIEKAENRDMFLSVVKDCNIPVPTGKAVTSIEAGKKAAKKIGYP
                     VLVRPSFVLGGRAMQIVNSDKDLLEYLTSAVKIDKDQPVLIDKYIVGKEVEIDAICDG
                     KDVYIPGIMQLVEATGVHSGDSMSVYPPYSLNDKVKKTIEEYTVKLGLAIGIKGLFNI
                     QFIVDKNNNVSVIEVNPRSSRSVPFLSKSTGVPMANIATKVMLGDSLKKQGYKTGVMP
                     EKNRFYVKSPMFSFSKLHGLDTYLSPEMKSTGEAIGYDDTLNRALYKSLHAANMRVVN
                     YGTVFVTIGDDDKTKAVELVRRFYNLGFNIEATKGTAKKLKSAGIKTRTKKKISEGSE
                     EIIESIQKGHITYIINTQSNRAGNKKKDGFLIRRAAVENGVTVFTSLDTVNVLLNILE
                     EITMQVSLIDD"
BASE COUNT         2231 a         1730 c         1634 g         2486 t
ORIGIN      
        1 ttggaaaagc gtgcgctaat gcgcttaagg atgctggagc aaaagttgtt attgtggatg
       61 ttagtgttga gactggcgat gtgattgatg gcatgaattg cattaagtgc gacatcacaa
      121 gccgtgaaaa cgtctatgca atggttgata aggttgttga gaacttcgat aaagttgaca
      181 ttcttgttaa taatgcaggg gttaatttcc ctcgtcttct tgttgatgtt aagggcgaag
      241 ctcctgaatt cgaggttaac gagaaagacc tcgacattat gtttaatatc aacgtaaaag
      301 gtctcttttg gtgtgctcaa gcttgcgcaa aggatatgct taagcgcaaa gaaggcgtca
      361 tcattaacat gtcttcagaa agtggcaaag agggaagtca aggcgcctcg atttacgcag
      421 ctacaaaagg tgcagttgat agtgctactc gctcttgggc taaagagctt ggcaagcata
      481 acattcgcgt agttgctgtg gctcctggaa tcatcgaagc cactgggctt cgcaacgatg
      541 cgtttaaaaa ggctttggct tatactcgcg gtgctaaaga ttacagcgag gttggcactg
      601 attattcgaa ggttataccg cttggacgcg atggaaaact tactgaaatt gcagaccttg
      661 tagcctattt agcaagcgac agatcaagct acgtctctgg taccactttc aacatttcag
      721 gtgcaaaatc acgtggttaa taaggttaag catacaagcg tagcgaagaa gtctgatacc
      781 ggcagcaaga ttgtcgactt ggctttagaa atagcttatt tgcaacttat ttcaggtgct
      841 gaaatggaac gtgttgatcg caccattcaa aaaatttgtg atgcctacga cgcgacaaat
      901 attgaatatt ttacctatcc tcgtttcatt attttgtctc tcgactacga aggtaaaact
      961 tacacaaagt caaaaaagat aagggacaaa gctccaaact ttcaccgttt ggaagagctc
     1021 tcaaaagttt tcgatgatat tttagattca aaaccagata tcgacattgt aattcagcga
     1081 gttaggatgc tttacacaac gaagcaattt ggcgtgctag cacaaatgtt ttgctatttc
     1141 ttcggacctg ctggctttac aattttcttc ggtggcaatt tttacgaagc ggcaatttct
     1201 ggtgttatag ggctattgat tttattgatt acctttggag ttaaaaagct gactgataac
     1261 tttttctttc caacggtaat ttatagtttt gttatgtctt tgagtgtcag aatattagga
     1321 gcgcttgcga tagttcaaaa tgttgaaagt atgcaaattg gtcttttgat gatggttgtg
     1381 ccaggtctga ttattactaa tagtatgcga gaatttatct ctggcgaata tttaactggt
     1441 tacagcaagc ttatcgaagc attgctcgtg gctttggcgg ttgcaatggg agtaatcttt
     1501 gctgctgcat tgatgaggat ttttgtataa tggaagagat gctgactaca tacataattc
     1561 cagttgctgc aatctttgtt gcctcaattg ctttttcaat tttttataac gttaagcgat
     1621 caagcatttt gctttgcgcc attggaggtg cacttggttg ggcagtttat cttgcttttg
     1681 taaacaacat cggtcataaa tacgtttcat tttttattgc ggctgcagcc atcacaattt
     1741 ttgccgaagt tgtggggcgc gtaaaaaatg ttcccgtcac cattttctta acaatttctc
     1801 ttattcctct tgtcccaggt agagctatat ttaataccat tgaatctctc ttaaagattg
     1861 ataagcaagg ctttatggat tgaggccttg agaccttctt gattgtgctt gcaatagcct
     1921 tcggagtgat ttttatatcc tcgtttatta gattgttctt taagcttcgc gagaaaaacc
     1981 tcgcaaaaaa acacgctaat taattcaact ttcgtcagaa ggattgtttc taaactggat
     2041 gattctgtct ttgtcttcgg gtttgatata gccttcttcg gcagctactt cgactaaaac
     2101 atccacattc gaagctgaga cattcttgac tttggcttct gccaggcgat caaccgactt
     2161 ttgcattcca taagtaaaga ttgaaactat acctaatact tcagcgccta cttcgcgaag
     2221 tgaatctaca acgtcaatgc agcttccgcc ggtagaaatt aaatcttcaa taacaacaac
     2281 cttggtacca ggctcacatt ttccttcgat tttattattg cggccgtggt cttttgagct
     2341 tccgcgaaca taacccatag gcaacttcaa aatatttcca acaagcgcag cgtgtgcaat
     2401 tccggcagtt gaggtgccca ttaccatttc gcattccgga tattgttctc gaacaatatc
     2461 tgctagtgca ttttcgatat cgtttcttac agcaatatca gaaagagtta atctgttgtc
     2521 gcagtatatc ggactcttaa ttccagatgc ccacgtaaaa ggctcatcag ggcgaaggaa
     2581 aacagccttg atttttagta agtcttttgc aattgtttct tctctgctca tattattctc
     2641 ctaagaaatc ttgaacacat ctgttatatg ctgcaacggg gtcggaggca gctgtgatgc
     2701 ttcgtccaac aactataaaa tccgaagaca ccttggcgtc agccggggta gtaactcgaa
     2761 cttggtcacc tttggcatca tcagcaaagc gaatgcctgg tgttaccgtt agaaattcat
     2821 ccccgatgga tttctttatt tctccagctt ctaaaggaga gcaaacgaca ccatcaaggc
     2881 cggcatcttt cgcattttgt gcgtaattta taacaacctc ttttatggtt ttggaattgt
     2941 ctaacaataa ctcctcatgc atcacttcct ctgaagtgct tgtaagctga gtaacagcaa
     3001 ttagcttagt agatgatcca tcaagagctt tttttgcagc ggccatcatt ttagtgccac
     3061 cagcgcaatg cacattagtc atatctacac caagctttgc cagctgcgaa gcagcctttg
     3121 agactgtatt agggatatcg tgaagtttta aatctaaaaa tattttatgt tcacgtgctt
     3181 taatttccct aacaatactt ggaccttcgg cataataaag ctccatccct atcttaacgt
     3241 aaggtttttc ttcggcaaat tggtcaagaa attcgtatgt ttcttgtgcc gaagaaaagt
     3301 cgcaagcaat aattatatct ttatccatta taaggacctc ccaacaatct ctgataggga
     3361 attaattcct aacttttcac atacttttgg taagtcttca attatttttt tgcatgcaag
     3421 aggatccgca caattcgcag cgccaacttc gacagcagta gcgcctgcgc tcatcatctc
     3481 tataatatct tcagcttttt ggatgcctcc gcaaccaact attggaatct tgactgcttg
     3541 agcaacctga tatacagcac gaagtgcaat aggcaggacc gcaggtcctg ataatccacc
     3601 tgtaacattg tccaagatag gccttccagt ttttatgtca aaccttgcac caagtactgt
     3661 gtttataagc acaaggccgt cagctcctgc gtctgcgacg ctagtcgcaa tatctacaat
     3721 atcagttaca ttaggtgtta atttgacata cacaggcttc ttgcaaactt tcttaacggc
     3781 tgatgtaatc tcggctgcaa gtttagcatc ggttccaaag gcagctccgc ctccatgaac
     3841 attagggcag gagatattaa gttccacgat tccaacggaa tcgtgagcat caagtttaga
     3901 agcaacatca acgtattctt cctttgagaa tccggagacg tttgctagta ttttcttcga
     3961 aaatacttcc tgcaattttg gtaattcggt gcgaagcacc tcgtctacac cggggttttg
     4021 taatcccact gagttcagca tccccgaagg tgtttctgca attcttggca actcgttgcc
     4081 atatcttgct tctttagttg ttcctttgat cgcaatagaa cctagaatgt taatgtcata
     4141 atgctcagca aattcgtatc caaaaccgaa ggttcccgag gcggggataa tagggttgtc
     4201 aagttttaaa ccagataagc taacacttgt atctaccata atagttcctc gctttcaaat
     4261 actggaccgt ccttgcaaat tcgtttcgga ccacctttag tttctatcga gcatcccata
     4321 caagctccga agccacagcc catgcgtgct tcaagtgata acttgccttt agaattcatt
     4381 gtatttacga tggccttttc cataggaagt ggaccacatg caatgtatgg aagatttgtg
     4441 aaaccttctt tcttaataat gtcagttacc aatccaggtt caccatagct tccatcatct
     4501 gttgaaatgt aaacctcgcc atattcttta aattcgtctt caagaattac ttcgtctttg
     4561 ctaagtgcgc ctaagataat ttttggtcga acgccaagtt catttaactt tttgcatgca
     4621 ccaagtagag gaggacagcc aattccgcca ccaactacaa gttgctcttc gcctgtaaga
     4681 ttaaatccgt ttccaagagg catcagcaca tccaagtttg cgcctttctt ttgttgagac
     4741 aatcaatctg taccaacgcc aacaacttta taaattagtg aaattgcatc atcgtctcag
     4801 ttgcaaattg atattggcct tcgtaagaat ttatcttcaa taagaatatt tacaaactga
     4861 ccaggctcag aaattgcatt agtgttacat gaagctacaa tcctaaatat atccttagcg
     4921 attttcgtgt tgcatagtat gtttaaattc tcattagtca tctatcaatg atacctgcat
     4981 cgtaatttct tctaatatat tcaaaagtac atttactgtg tcaagcgaag taaataccgt
     5041 aactccattc tcaacagctg cacgacgaat aaggaacccg tctttcttct tattgccagc
     5101 tctgttcgat tgcgtgttga taatgtaagt gatatgtccc ttttgaattg actcaataat
     5161 ctcttcggag ccttccgaaa tcttcttctt agtgcgagtt ttaattccgg ctgatttcaa
     5221 cttcttggca gtgccttttg tggcttcgat gttaaagcca agattataaa agcgtctaac
     5281 aagttcaaca gctttggtct tgtcgtcatc gccaatcgtg acgaagaccg ttccgtaatt
     5341 tacaacgcgc atgtttgcag cgtgaagaga tttgtaaagc gcacggttaa gcgtgtcatc
     5401 gtagccgata gcctctccag tagatttcat ctcaggcgag aggtaagtat caagaccatg
     5461 aagcttactg aagctaaaca tcggactctt aacatagaag cggttcttct caggcataac
     5521 gccagtctta taaccttgct tcttaagtga gtctccaagc ataacttttg ttgcaatgtt
     5581 cgccatcggt acaccagttg actttgaaag gaatggaacc gaacggctcg agcgagggtt
     5641 aacttcgata accgaaacat tgttgttctt gtcaacaata aattgaatgt taaacaagcc
     5701 cttaatgcca atcgcaagac caagtttgac cgtgtattct tcgatagttt tcttaacttt
     5761 atcatttagc gaataaggag ggtagacgct catgctgtct ccagagtgaa caccggttgc
     5821 ctcaacaagc tgcataatgc ctggaatata gacgtctttg ccatcacaaa tagcgtcaat
     5881 ctcaacttct ttaccaacga tgtatttatc aataagtact ggctgatctt tgtcaatctt
     5941 aaccgccgaa gttaagtact caagcaagtc cttgtctgaa tttacaattt gcattgctcg
     6001 gccaccaaga acgaagctcg ggcgaaccaa aacagggtag ccaatcttct tcgcagcttt
     6061 tttgccggct tcgattgaag taacagcctt gccagttgga accggaatgt tgcaatcctt
     6121 tacaacgctt aggaacatgt cgcggttttc ggccttctca atcgcatcaa catccgtacc
     6181 aataattggg actttatatt cctttaactt ggttgagagg ttaacagcag tttgaccgcc
     6241 aagagaaaca atgactccat caggtttttc aaggtcgata atgtgcatga cgtcttcaac
     6301 gcaaagcggc tcgaagtaga gcttatcgct cactgcataa tcggtcgata cagtttcagg
     6361 attgttgttg ataacgatag cttcgtagcc tttttgctga atagcccaaa ttgcgtgaac
     6421 tgtcgagtag tcaaattcaa caccttggcc gatcctgata gggccagaac caagaacgat
     6481 aatcttttta cgcttgctga caaccgattc gttttcgtct tcgtaggttg aatataggta
     6541 agggacatca gcgataaatt cagcgccaca ggtatcaatt tgcttataaa ccggcaatat
     6601 gtggttgcgc ttcgtatagt cgtagaactg cttgtccttc atcttataaa ttggagcgag
     6661 atattcattc gagaagccga actccttaca cttgcgaaga ttcttaacat tcggcgcatc
     6721 tttaaccttc ttctcaaggg atacaatgtg atgtatttta tccaggaaga atgggtcaat
     6781 ttgcgttgcg tgattgattg ccatgaggtc aatcttgcga cggaaacatt cagcaattgc
     6841 gaagaagcgg tcgtcgcttg gcgttttaat aaagtcaaga agttccttat tgctaaactt
     6901 gtcaaacttc tcaagatata ggtggttaac gccaatctca agcgaacgaa tcgcttttag
     6961 taaactttct tcgaagttac ggccaatgct catgacttcg ccagttgcct tcatttgagt
     7021 gttgagctca tgatcggcgc tctcaaactt atcgaagggg aagcgcgcaa ctttgcaaac
     7081 aacataatct acgcttggtt caaaagcagc acaagtgctt gcaagttgaa tttcttccaa
     7141 atgcataccc acagcaatct tgctgctaac gcgagcaatt ggataagctg ttgccttcga
     7201 agcaagtgcc gaagatctgg acacgcgcgg gttaacttcg ataacataat attcaaaact
     7261 aaatgggtcg agggcgaact gaacgttaca tccgccttcg atatcaagct ctgtaattag
     7321 ctctagggct gcatttgaga gcatgtgata ttctttgtca gacagagtct gaaccggagc
     7381 aacaaccatg ctgtctccag tgtgaactcc aacaggatca acattttcca tgctacaaat
     7441 tacaattgca gtcccgttag aatcgcgcat aacttcaaat tcaatttcct taaagccctt
     7501 aatggacttt tctacaagaa cttgagttac aggagaaatc ttgagtgcat tcttcgcaat
     7561 ttcttcaagt tcctgatcgt tataaacaaa tccaccacct gttccaccaa gtgtaaatgc
     7621 aggacgaaga acaatagggt agccaatttc gttggcaatc tcgcgagctt cttcaacggt
     7681 gtgagcaatt tcagactgaa gacaaggctg gccaattctt tcgcaaagtt ccttaaagcg
     7741 aagtctgtct tcggcgttat ttatagagtc aaaacttgtg ccgagaattt cgcatttgca
     7801 ttcatccaaa ataccttttt ccgcaagctt cattgccatg ttgaggcctg tttgacctcc
     7861 aagcgaagga agcagcgcat caggacgttc tttacgaatg attcgagcga tgtattcaag
     7921 ctttagaggc tccatgtaaa ctttgtctgc aatttgtgta tctgtcataa ttgttgcagg
     7981 gtttgaattt acaagaacaa cgctatagcc ttcttcttta agggcaaggc aagcttgagt
     8041 tccagcataa tcaaattctg cagcttgacc aataacaata g
//
LOCUS       DBNCDC010000049         8047 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_4281_length_8047_cov_5.069820, whole genome shotgun sequence.
ACCESSION   DBNCDC010000049 DBNCDC010000000
VERSION     DBNCDC010000049.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 8047)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8047)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8047
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4281_length_8047_cov_5.069820"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1451
                     /locus_tag="AC04VQ_04550"
     CDS             <1..1451
                     /locus_tag="AC04VQ_04550"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006538 - L-glutamate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019665 - amino acid fermentation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HIO9553430.1"
                     /translation="IPEGDFEKERQEVLATWHTGKEVDLKEAFEYQKQITGDRRFSNK
                     LAEAKRVKKTLTQPRAGVPVLEEHVKLMQYLEKYGKVDLLPSTIDSYTRLNRYDAAEK
                     GIKSSIIEGRALMNGLPVVNHGVRQCRTIIESVNTPIQVRHGTPDARLLTEVAYAAGF
                     TSYEGGGVSYNLPYCKNIPMEETIKTWQYTDRLTGLYAENGIIINREPYGPLTGTLVP
                     PCISHAVAVIEAILAAEQGVKDITVGYGQCGNITQDVAAIQSLEQVTNEYLEKYGYGD
                     VEVTTVLHEWMGGFPEDEAQAFAVISLGAACAALSKATKVIVKSPHEAVGIPTMEANA
                     EGCRATKQVINMLYEQNYTASNLEEEREMILRETRQILEKTYELGEGDWAVGCCRAVE
                     AGVIDVPFAPCAANAGLVLPARDNDGAVRIMDMGNLPFDDDIKTFHKDKMEKRAKFEK
                     RDVHFNMVVDDIYAIGRGHIVGRPEYSQKDKK"
     gene            1477..2727
                     /locus_tag="AC04VQ_04555"
     CDS             1477..2727
                     /locus_tag="AC04VQ_04555"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006538 - L-glutamate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019665 - amino acid fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HIO9553431.1"
                     /translation="MKIVDVVCSPGRTGFYFDDQRAIKQGAGHDGIFYTGTPVTEGFT
                     SIRMAGESISVQIVLEDGQVAYGDCAAVQYSGAGGRDPLFLAKDFIPIIEEHIKPTLI
                     GKKADNFRKLAEDMEKIEVNGKRLHTAIRYGVSQALIDAVARANHKQICEVVADEYEG
                     CDLKPELVDIFTQSGDDRYDGADKMIIKGAQVLPHALINNVEDKLGKQGEKLLEYVGW
                     LRDRILNNRTDDSYNPIFHIDCYGTIGAVFGNNNYKEMADYIEKLGEQAKPFHLRIEG
                     PMDCDTDRETQIEALAGLTKEIDDRGIDVELVADEWCNTLDDVKAFTDAHAGHMAQIK
                     TPDLGGINNTIEAVLYCKANGMGAYQGGTCNETDRSARILTQCALATRPKQILAKPGM
                     GVDEGYMLVYNEMQRAVALLKSKK"
     gene            2821..3090
                     /locus_tag="AC04VQ_04560"
     CDS             2821..3090
                     /locus_tag="AC04VQ_04560"
                     /inference="COORDINATES: protein motif:HMM:NF015997.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HIO9553432.1"
                     /translation="MENSERFFQNKDCKFFPCHKTDDPENFNCLFCYCPLYALGDKCG
                     GNFKYIGGVCKDCSDCTRPHDPANYDYICSRYDEIMDLAAKNHKD"
     gene            complement(3120..3362)
                     /locus_tag="AC04VQ_04565"
     CDS             complement(3120..3362)
                     /locus_tag="AC04VQ_04565"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIO9553433.1"
                     /translation="MNLIYEKDYPPINCIPSGHTMACTAMIFGTVLCKKMKPSTKSII
                     IVINVLTICSTVFTKQHALIDLVFAFVYTTVIFVVV"
     gene            complement(3859..4350)
                     /locus_tag="AC04VQ_04570"
                     /pseudo
     CDS             complement(3859..4350)
                     /locus_tag="AC04VQ_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013155.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            complement(4657..5007)
                     /locus_tag="AC04VQ_04575"
     CDS             complement(4657..5007)
                     /locus_tag="AC04VQ_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553434.1"
                     /translation="MKKKFEQLKQDVWQNFSENDLYITSILKSPKLYDTELYKFCEAM
                     PKGADLHSHGGAMTPVRHLIDFVISKEELLIDTDPEHKGYLQLANKNPGASYMPLRQA
                     LDEGLLTKDELFKS"
     gene            5091..5648
                     /locus_tag="AC04VQ_04580"
     CDS             5091..5648
                     /locus_tag="AC04VQ_04580"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HIO9553435.1"
                     /translation="MAGINDPVFRAICIDYGAMLTYTEMVSSKALSYKNEKTKNLLQL
                     ASNEDKVVVQLFGHEPKTIAQEAHNIELELGKRLAYIDINMGCPARKITKKGDGSALL
                     KDQKLATDIVSTCVDACDIPITVKIRKGFEEDEDISLEFAKAMECAGASAVCVHGRTA
                     KQFYKGKADLSCGENLVRELEIPVI"
     gene            5981..6757
                     /gene="hemW"
                     /locus_tag="AC04VQ_04585"
                     /pseudo
     CDS             5981..6757
                     /gene="hemW"
                     /locus_tag="AC04VQ_04585"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
     gene            6800..7090
                     /locus_tag="AC04VQ_04590"
     CDS             6800..7090
                     /locus_tag="AC04VQ_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553436.1"
                     /translation="MTQNSERRMRVTDEHVEDDLNANQMLAEDCMLAMRTRFGISAEL
                     ANKARGQLKNFNTTIEDLIKAGLVKESSGAYVPTEKGWLCGNELYIKLMDLC"
     gene            7106..>8047
                     /locus_tag="AC04VQ_04595"
     CDS             7106..>8047
                     /locus_tag="AC04VQ_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009608625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HIO9553437.1"
                     /translation="MAKDLYEILGVDKNANEQEIKKAYRRRARELHPDVSEDPNAEEK
                     FKELNEAYDVLSDPKKKAQYDRFGTIPGAASGPGGSPFGGGYVDFEDLFGGGFGINDI
                     FSSFFGGMGGMQNNIEGRDMSVGLRLTLEEVATGTSKEIVYDRLAPCEDCDSTGMGKD
                     GKKVTCPTCHGEGKVVSVQQTFLGTMQTQTVCPECRGAGESIEGACPECEGQGRVPDR
                     QRVSVEVPAGIRDGQQLRVNGFGEAGVRGRRSGDLIVTCRIMPHEFFERDGNNLLARV
                     NVSFIQAILGAEISVDGIFKDEKVPLKVPKGTQNGDVL"
BASE COUNT         2460 a         1410 c         1756 g         2421 t
ORIGIN      
        1 aaattcctga gggcgatttt gaaaaagagc gccaagaggt cttagctacc tggcatacag
       61 gtaaggaagt tgacctcaaa gaagcttttg aatatcaaaa gcaaattact ggtgataggc
      121 gtttttctaa caaacttgct gaagcaaagc gtgttaagaa gactcttact cagcctcgtg
      181 ctggtgtacc agttttagaa gagcatgtta aacttatgca gtacttagaa aagtatggca
      241 aggtagactt gcttccttct acaattgaca gttatacacg tttgaacagg tatgatgccg
      301 ctgaaaaagg tattaaatct tctattattg aaggtcgtgc tttaatgaat ggtttgcctg
      361 tagttaacca tggtgttcgt caatgtagaa ctattatcga gtctgttaat acaccaattc
      421 aggttcgtca cgggacgcct gatgcaagat tgctgacaga agttgcttat gctgctggtt
      481 ttacaagtta tgaaggtggt ggagtttcct acaacctgcc atattgtaaa aatattccta
      541 tggaagaaac aattaaaaca tggcagtata cagatcgctt aactggtctt tatgcagaaa
      601 atggcatcat tattaatcgt gagccatacg gtccattaac tggtactttg gttcctcctt
      661 gtatttctca tgccgttgct gttattgagg caattttggc tgctgaacag ggcgtaaagg
      721 atattacagt tggttatggt caatgcggaa acattactca ggatgttgct gctatccaaa
      781 gtttggaaca ggttaccaat gaatatcttg aaaaatatgg ttatggcgat gttgaagtta
      841 caactgtatt gcatgagtgg atgggtggtt tccctgaaga cgaagctcag gcttttgccg
      901 ttatttcttt gggtgctgct tgtgctgcat tgtcaaaggc aacaaaagtt attgttaaaa
      961 gcccacatga agctgttggt attcctacta tggaggcaaa cgctgagggt tgtcgtgcta
     1021 caaagcaggt tattaatatg ctttatgaac aaaattacac tgcttctaac ttggaagaag
     1081 agcgcgagat gattcttcgt gagactcgtc aaatcttaga aaagacttat gagcttggcg
     1141 aaggtgattg ggctgttggt tgctgcagag ctgttgaagc tggtgttatc gatgttcctt
     1201 ttgcaccatg tgcagcaaat gcaggtcttg tacttcctgc tcgtgacaat gatggcgctg
     1261 ttcgtattat ggacatgggc aaccttccat ttgatgacga tattaaaacc ttccataaag
     1321 ataagatgga aaaacgtgct aagtttgaaa agcgtgacgt tcacttcaat atggttgttg
     1381 atgacattta cgctattggt cgtggtcaca ttgttggccg tccagaatat agccaaaaag
     1441 ataagaaata atataagata ttaaggagga gaaaaaatga aaattgttga tgttgtatgt
     1501 agtccaggac gtacgggatt ttacttcgat gatcagcgtg ctattaagca aggtgctggc
     1561 catgatggta ttttctacac tggcacacct gtaacagaag gctttacttc cattcgtatg
     1621 gctggtgaat cgatttctgt tcagatcgtt ttagaagacg gtcaagttgc ttatggcgat
     1681 tgcgctgctg ttcagtattc aggtgctggt ggacgtgatc cattgttctt ggctaaggat
     1741 ttcattccaa ttattgaaga gcacattaag cctactttaa ttggtaaaaa agcagacaac
     1801 ttccgtaagc ttgcagaaga tatggaaaaa atcgaagtta acggaaaacg tcttcacact
     1861 gcaattcgtt atggtgtaag ccaagcattg attgatgctg ttgctcgtgc taaccacaag
     1921 caaatttgcg aagttgtagc tgacgaatac gaaggctgtg atcttaagcc tgaacttgtt
     1981 gacatcttca ctcaatctgg cgatgaccgc tatgatggtg ctgataagat gatcatcaag
     2041 ggtgctcaag ttcttcctca tgcattaatt aataatgtag aggacaagct tggaaagcaa
     2101 ggcgagaagt tgttagaata tgttggttgg ttacgcgaca gaattcttaa caacagaact
     2161 gatgatagct ataatccaat tttccatatt gactgctatg gaacaattgg tgcagttttt
     2221 ggaaacaata actacaaaga aatggctgac tacattgaga agttaggcga gcaagcaaag
     2281 ccgttccatc ttcgtattga aggcccaatg gattgcgata ctgatcgtga aactcagatc
     2341 gaagctttgg ctggtcttac aaaagagatt gatgaccgcg gcattgacgt tgagcttgtt
     2401 gctgatgaat ggtgcaatac tcttgacgat gttaaggcat ttactgatgc tcatgctggt
     2461 catatggctc aaatcaaaac acctgatctt ggtggtatta acaatactat cgaggctgtt
     2521 ttgtattgca aggcaaacgg aatgggtgct tatcaaggtg gcacatgtaa cgaaactgat
     2581 cgttctgctc gaattcttac acagtgcgct ttagctactc gtccaaaaca aatccttgca
     2641 aaaccaggta tgggtgtaga cgaaggctac atgttagttt ataacgaaat gcaacgtgca
     2701 gttgcacttc tcaaatccaa aaaataatta atatatttgc cccggggtca acactctatt
     2761 tccccggggc cagcactcga tttactcgtg gcatttactc gattttagaa agataccatt
     2821 atggagaaca gcgagagatt ttttcaaaat aaggattgta agttttttcc gtgtcacaaa
     2881 actgatgatc ctgaaaattt caattgcctt ttttgctact gtccgcttta cgctttaggg
     2941 gataagtgtg gcggcaattt caaatacatt ggtggagttt gcaaagattg cagcgattgc
     3001 actcgacctc atgaccccgc gaattatgac tacatttgtt cgcgttatga tgaaattatg
     3061 gaccttgctg ctaaaaatca caaagattaa gatttcttat acactttata atagatcaat
     3121 caaacaacaa caaatatcac agttgtataa acgaaggcaa acacaagatc gataagcgcg
     3181 tgttgtttcg taaataccgt tgagcatatt gttaaaacat taataactat aattattgat
     3241 tttgtcgaag gtttcatttt cttacaaaga acagttccaa aaatcattgc tgtgcatgcc
     3301 attgtatgtc cgcttgggat gcagttgata ggagggtagt ctttttcata aattaagttc
     3361 atcaatcagg taaagaaatc agttgtttta agctccggaa tttctacaat tgtcggatat
     3421 gctataaatg ttattaaagc tgcaatgaac atcaaattgt aagttattat ataaacatgg
     3481 aacaaacccc ggtctcttcg tgaaatcata aaaggcacaa ttcaaatcat cgcatatcaa
     3541 ataacataaa atacgacgaa gtatttgata aacggaattt ggttgtcgat attactatta
     3601 accacatgca agtcagtaag agcatattta ctaataaagt atgtgccaaa ttgccaaatt
     3661 aatgtaaggg caattagcaa aagagcaact caatcagcct ttttaaaggt gaaagttttt
     3721 ttaatctttt caatcattga caaacttctc tcaatctttt tgtcatagtt taagcttatt
     3781 aactttattt tttcggttca aaaatgaata ctttattgaa ttagaagaaa gttgcacaac
     3841 ttcgtcaagc tttaaatctc aagcaagaat agtcataaaa aaatcatctg ttaggctgtt
     3901 gtattcttga taggtagggt catctgaaca aagagcaata gggatgccct cacgaagata
     3961 atctttcccc ggatgattgc gaaggtcatc acaaaatctc aaactttggt tgcttatcgg
     4021 acaaacctca agacaaactc ccttattttt aattgtgttt tttagttcag gatgcgctca
     4081 caaatttagg ccgtggccaa ttctttttgc gtctaggttc aacgcttttt ttatctcgtt
     4141 gttgtttggc gaagtactct caccagcgtg taaggcaaca ttaagccctg gagcattttt
     4201 aagtgttttg tggattaggt tttcaaactc cgaaattgaa cgactggaat cttcttcgtt
     4261 aaccaggtca atcccaacaa aaaagtcatc gccgttatcc ttaatttgat gatgcatttt
     4321 tacagtattt tgatagattt cttcagtcac atctaaatca aaaatattgt ttttaagccc
     4381 aacaggaata gcgcgaacgg tcaagtttgg atattttttc ttcgtttcaa tataagcatc
     4441 gaagacgcag cgagcctttt tcgaagcgtc actttcacta ccttttaaaa acaaacgaat
     4501 ttcaatatgc gaaatatttt gcgagcagta atattcaaat gcattttgaa agtaatcttt
     4561 gcaagttgga ccaatttcgt ttaagtcatt atgctttacg aagaggtctt gaaatcagtc
     4621 tcatatatcc atgttgtttg ggcaaccaat tagtgttcaa cttttgaata gctcgtcttt
     4681 ggtaagcaaa ccttcgtcta acgcttgtcg aagaggcatg taagatgctc ctggattttt
     4741 attggcaagt tgaagatatc ccttatgctc aggatcagta tcaattaaaa gctcttcctt
     4801 gcttataacg aagtcgatca agtggcgaac aggtgtcata gcaccaccat gagaatgcaa
     4861 gtctgcacct tttggcatgg cttcgcaaaa cttgtaaagc tcggtatcgt aaagcttggg
     4921 actttttaaa attgaagtaa tgtagagatc gttttccgaa aaattttgcc aaacatcctg
     4981 ttttaattgt tcaaactttt tcttcatatt tcgtattata ataaaacgct aatattatgt
     5041 gcaaagattt aacaaaattt tttagcaatc gctggcttat gcttgctcca atggcaggga
     5101 ttaacgaccc tgtttttcgc gcaatttgta ttgattatgg agccatgctg acctacaccg
     5161 aaatggttag ttctaaggct ttgtcttaca aaaacgaaaa gaccaaaaat ttacttcaac
     5221 ttgcaagtaa cgaagataag gttgtcgttc aactttttgg ccacgaacct aagacaattg
     5281 ctcaagaagc ccataacata gaacttgaac tgggcaaacg acttgcttat atcgatatta
     5341 acatgggttg tcctgcgaga aaaattacga agaagggtga cggctctgct ttgctcaaag
     5401 atcaaaaatt agcaacagac atagtttcga cttgtgttga tgcttgcgac attccaatta
     5461 cggttaagat tagaaaaggt tttgaagagg acgaagacat cagcttggag tttgccaaag
     5521 caatggagtg cgcgggagct tcggcggttt gtgtccacgg aagaacggca aagcaatttt
     5581 ataaaggaaa agcagacctt agttgtggag agaatttagt tagggaatta gagattcctg
     5641 ttatttgaag tggagatgtg gattcttaca ataaggcgaa agagcttaaa tccaaaggtt
     5701 ttacagcaat tatgatagga cgcgccgcaa gaggaaaccc ttgatgcttt tcgcaaaatg
     5761 ctacttatag tatgcaagat aaaattagcg ccgcaaagaa gcaagtcatt aactacgaaa
     5821 aacttttccc taaaaattta gcccacatga gaaaacattg caattgatat gtccacggaa
     5881 tgcctggagc aacatttgca agagacaaat tctcccattg tgatacagta gatgattata
     5941 tgaaggtttt tgatgaacta ttagagaggt tggaaaacta atggcgtttg atccttataa
     6001 agccctatat ttacacattc cattttgtgt tcaaaagtgc aattattgcg atttttgtag
     6061 tcgtgctgct gatgttgatg gcgcagaagt tcgcgaatat gttgagcact tggttcaaga
     6121 tattcgcaaa gcttcgaagg aagataagct tggaagtgtt gaaacaattt atattggagg
     6181 aggaactcca agctatatag gaaccaaaaa tctcagtagt attttatacg ccctttctat
     6241 gtctatgaga cttgaaaagg ttagtgagtt tagcatggaa gcaaacccag aaagtatcaa
     6301 cgaggcttta attaaagata tttgagcaat gggagttaat cgtttatcgc ttggtgtcca
     6361 gtccttcgat gatagtatgc tgcaaatgct tggtcgtgct cacgattcta agactgcaat
     6421 tgaagcaatt gaaaccgtcc gttcaaggtt taaaaatttt agcatcgatt taatgtgtgg
     6481 cattccaggt cttagcgaag acgtttttag ggaaagcgtt caaaaagcta ttgacttaaa
     6541 agtccctcac gtaagcattt atccgctaac aattgaatac aacacaatgt tttataaatg
     6601 gaaggcccaa ggcaaaattg acgatattga cgaagacgtt caggccgacc atatgttgct
     6661 tgctgcagag atgcttgaaa atgctggata taaacattac gaagttgcaa gttatgctct
     6721 tgatggccat gaatgcaaac acaatttagc ctactgacaa ggcaagcctt atttaggtct
     6781 tggtgagagt gctacaacaa tgactcaaaa ttccgagcgc cgaatgagag tgactgatga
     6841 acatgtagaa gatgatttaa acgcaaatca aatgcttgct gaagattgca tgctagcaat
     6901 gagaacgcgc ttcggtattt ctgcagagct tgcgaataaa gccaggggtc aacttaaaaa
     6961 ctttaacaca acaattgaag atctgataaa agctggcctt gttaaggaaa gtagtggtgc
     7021 atacgttcct acagaaaaag gatggctttg tggcaatgaa ctttatatta aattaatgga
     7081 tttgtgttag aatacccact tgcttatggc aaaagattta tatgaaatat taggtgttga
     7141 taaaaacgcc aacgaacaag agatcaagaa ggcttacagg cggcgtgctc gtgagcttca
     7201 tcctgatgtc agcgaagacc ctaacgctga agaaaagttt aaagaattaa acgaagctta
     7261 cgacgtttta tctgatccga agaaaaaagc acaatacgac agattcggaa ctattccagg
     7321 tgccgcaagt ggtcctggag gcagtccttt tggcggcgga tatgttgact tcgaagattt
     7381 atttggtggc ggttttggaa tcaatgacat ttttagtagc ttcttcggtg gcatgggagg
     7441 catgcaaaac aacatcgaag gcagagacat gtctgtggga cttcgtctca ccttagaaga
     7501 agttgctact ggaacttcga aagagattgt ttatgacagg cttgctcctt gcgaagactg
     7561 cgattcaacc ggaatgggta aggatggtaa aaaggtaact tgtccaactt gtcacggcga
     7621 aggtaaagta gtttctgtcc aacaaacatt tttagggaca atgcaaactc aaactgtatg
     7681 ccctgaatgt aggggtgctg gcgaatctat cgaaggtgct tgtccagaat gcgaaggcca
     7741 gggtcgcgtt cctgatagac aaagagtttc tgttgaagtt cctgctggca ttcgtgatgg
     7801 tcagcaactt cgcgttaatg gatttggcga agctggagtt cgtggcagac gaagcggtga
     7861 tttaattgtc acttgcagaa taatgcctca tgaatttttt gaacgtgatg gaaacaatct
     7921 tcttgctcgt gttaatgtat cttttatcca agctattctt ggtgctgaaa taagcgttga
     7981 tggaatattt aaagacgaaa aggttccact caaggttcct aaaggcacac aaaatggtga
     8041 cgttttg
//
LOCUS       DBNCDC010000050         8037 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_4291_length_8037_cov_6.553495, whole genome shotgun sequence.
ACCESSION   DBNCDC010000050 DBNCDC010000000
VERSION     DBNCDC010000050.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8037)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8037
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4291_length_8037_cov_6.553495"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="AC04VQ_04600"
     CDS             complement(<1..279)
                     /locus_tag="AC04VQ_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553438.1"
                     /translation="MGIAALFVGIYIIGNDKFEIINLKKEYESLEKTLLFYKLTGELQ
                     ERYISLMIKHTNDVISKIRLYTNIIFVIGISLGCSLFSYYISEILNPNN"
     gene            738..1361
                     /locus_tag="AC04VQ_04605"
     CDS             738..1361
                     /locus_tag="AC04VQ_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553439.1"
                     /translation="MRSSNYRFNKKGAHAGKMSWQRMPLSKCVISIVLTITLVLGFGT
                     NLSYAAEFLPEGPQTVTMSNAETKTLVAPASNYDQANYTWEMFSNELGDFAQISGETG
                     STLKVDPGYFGEEATSVQIKCTVSDANSSASTSQVYNVNLIRENNSVDEALAAATANE
                     EGATTVDQTNNDSEVNEKIPVTVKYIDETTKKEIAKPTTSEFETAFT"
     gene            2442..3743
                     /locus_tag="AC04VQ_04610"
     CDS             2442..3743
                     /locus_tag="AC04VQ_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553440.1"
                     /translation="MSTTNHTTKSESSDNAAFRKVDSRSSYRVNLGYMLQKIDAETSH
                     NTIKRVATNPSDEANYEVFHSVSVCYYSSYYGTMVFSNDEYIPIEGFTTPTAGTSDGP
                     ALQTNIAWTDGQGNSHGYNLYYKRNKHDISLNRIGGAYNYKNIEGVYYDKVINTVLVP
                     ESWVNTEKIGYWANPSAQPINTLDNFIPYNPGGKVADQNFIFGGWTMDQTGQGDAVDL
                     SQINMPNAGLNLYAKWVAPEVKVTFVETDDPLQPETEFKTIDFDNVTYNHKLEEYATA
                     EKPIPQIKNFYDESEVMTYEGQARYYYENDAARKAGLKTYWNPYEMSIPGHNIKVYTE
                     YANVKTVTYTVQYVLNVDPTTEVAAERTGVGYEGSSVVLYPKVNNELYFDYRATYVPT
                     QDKMTVTVTDQAVFTFKYEVAKDVNLRYNAYYLNKDKLPGS"
     gene            3804..3995
                     /locus_tag="AC04VQ_04615"
     CDS             3804..3995
                     /locus_tag="AC04VQ_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553441.1"
                     /translation="MVNTVDVEALEFDNLTRDADTPDIQTVVLNVNNDNNVFFYYNEN
                     VVDINYAVKIDGEVVPKAD"
     gene            4380..4709
                     /locus_tag="AC04VQ_04620"
     CDS             4380..4709
                     /locus_tag="AC04VQ_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553442.1"
                     /translation="MQAIGDQYEMQWFTFESWSINGSGKYVSASGTLKEDTIFKASFN
                     NTTLKCEFKVKDNRGGEVLTSQIYVPYNAKCKLVNDSSLQFYDSSDNPIGGLISGKEN
                     DHFDFKK"
     gene            5061..5285
                     /locus_tag="AC04VQ_04625"
     CDS             5061..5285
                     /locus_tag="AC04VQ_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553443.1"
                     /translation="MLFNSTCTANFSQLLYDVSFNVSPEGSGSVEVDTPLLSQVPYGT
                     TFAPDATLPSKLMFNVDGQQCAASALPSEY"
     gene            5592..6071
                     /locus_tag="AC04VQ_04630"
     CDS             5592..6071
                     /locus_tag="AC04VQ_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553444.1"
                     /translation="MVAEETTTIGKDATLVVNLSYDEITVHFGSSDAHKGIASREEGS
                     SDGVDVKVPYYTTFVINSQNGYVDVKDALKQFVKVFPQSYDTGEQYLFRNWTLNSTAL
                     ATDTPITIDTPNAQYLCNFDLVEYPVSFVNVYGTTEVPRGTIEVKKGGETVDDLTLP"
     gene            6414..6965
                     /locus_tag="AC04VQ_04635"
     CDS             6414..6965
                     /locus_tag="AC04VQ_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553445.1"
                     /translation="MQKVTLDTHEDAVKKTNEDPDFEYYLVDNDHVPVQQFYIPIEDY
                     ENIPEVNYYYPRQEFKLTINYVINDESADAPDAPDPYEGNIAWGVKYGIASPTLEGYV
                     ADITVAEGTMPTEDREVTVTYTKYVPPTPPEPTPESEDINAQTGDSSLGLVGILVAIV
                     AIAGVGLFANRRRYDVRGKHSIR"
     gene            7026..7583
                     /locus_tag="AC04VQ_04640"
     CDS             7026..7583
                     /locus_tag="AC04VQ_04640"
                     /inference="COORDINATES: protein motif:HMM:NF028039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HIO9553446.1"
                     /translation="MKHGKKNINKKVIIPLAVVVAVLVFGLIYASCVKLINLTPYAAQ
                     PSNFTVADDFDISIKGTSITYEEPASSDNILINANVKNNSEEGWDLVSCFYINAKQDS
                     SKMLVEPEIERTDGLANNTTALGTYIGAGESAEIQLAFRLSDYSNVTLTFQGFPYNEK
                     TFEPVDVVLPVHKTVEVWWNEICNW"
     gene            7677..7835
                     /locus_tag="AC04VQ_04645"
     CDS             7677..7835
                     /locus_tag="AC04VQ_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553447.1"
                     /translation="MASVFKNPKFPSAQITMGAASSMEAKALAEQTAQMSNIKDPVQS
                     FEIKGKIY"
BASE COUNT         2574 a         1386 c         1577 g         2500 t
ORIGIN      
        1 attgttaggg tttaatattt ctgagatgta gtaactaaat aaagaacatc caagcgatat
       61 ccctattaca aaaataatat ttgtgtacaa tcgaatttta ctgatcacat cattagtatg
      121 ttttatcatc aatgatatat atcgttcttg taattctcct gtcaatttat aaaataataa
      181 tgtcttttca agagattcat attctttttt aaggtttata atttcaaatt tatcgttgcc
      241 aataatatat atgcctacaa acaaagcggc aattcccaaa ataattaata atataatagg
      301 tatagaataa ttgttaattt tttgaaatat aatcgctaga gtaataaatg aaaggcttat
      361 agaacaaaaa actaaagcta aaatacataa ttcataaata aaatgtaaaa ttctaaagaa
      421 gtatcttctt cgaaagtaat gattaaccaa atatcgatca tttaatttaa taactgatgg
      481 atcaattaac cttatttcat taaatgtata catatatacc tttattatta tacaaataac
      541 tctaattaat acaacaatta agtctatcct ttatattatt cagtagaatt tttattttct
      601 cggaatccac actcgatttc cccgtggcaa aaactcgatt ttcagctgat tttatgccga
      661 ttttcttgca tttatgataa aatatatatg ttttgggaat tcccaaaatc tttgataatg
      721 taaagaagga gaaaaaagtg agaagttcta attatcgatt caacaaaaag ggggctcacg
      781 caggcaaaat gtcatggcaa cgcatgccgc ttagcaagtg tgtgatcagc atcgttttga
      841 ctatcacgct ggtccttggc ttcggcacta acctaagtta tgcggccgaa ttcttaccgg
      901 aggggccgca gaccgtcacg atgtcaaatg ccgaaacaaa aaccctggta gcgcctgcaa
      961 gcaattatga tcaggccaat tacacatggg agatgttttc gaatgagctt ggcgatttcg
     1021 cgcaaatcag cggcgaaact ggctcgacgc tcaaggttga cccaggttat tttggcgaag
     1081 aagcaacttc ggttcaaatc aaatgtaccg ttagcgatgc aaacagcagt gcttcaacct
     1141 cgcaggtcta caacgtaaat ctcatccgcg aaaacaatag cgtcgacgaa gccctagctg
     1201 cagcaacagc taacgaagaa ggcgccacca ctgttgatca gacaaataat gattctgaag
     1261 tgaatgaaaa aattcctgtt actgtcaaat atatcgacga aacaacaaaa aaggaaattg
     1321 caaaaccaac aacaagtgaa tttgaaacag cgtttacatg aaaacttcaa attaagaact
     1381 ttcttggtga acctaagtct tgctcagtaa ccactcctaa cccacaaatt actgcacaaa
     1441 tttctggctc aataggtgca tgaaccattg atgtagaagc aacatcttct cttacggccg
     1501 ctcaagaaat tgttgtagga tttgataaat cacaggctca atatcaagta tattgttatg
     1561 accaaaatgt tgatggctcg ttcccttcaa cacctaatga cgggcgaatt tttacaaata
     1621 ctggccttgt aggaaccgac gttccggcat cagctgaaga agctgctgaa tatatgatta
     1681 aaggtggtgg ctgagggttt tctacatata ttcctacaac tatcactttt gacagcagtg
     1741 caaagtgtgc gatttatttt tatagaacat ttagtcttgt aagttttgat tttgcagaag
     1801 gcattaatgg cccaagggca atttttgcac gccaaggttc tttattgacg gttgcagaat
     1861 ctgataatcc aattagaccg ggttatactt tccaaggttg aactgacaaa gcaggtaaac
     1921 caatgccaaa tactattcct gttgctaata ctacttacgt agcaaattga aaagcaaatg
     1981 gaagatatgc taacgtgtat ttggtttatt gaggtgaaaa tgctgatgac gacggatatg
     2041 gttatcttcg tgaaaatatt gatgcatatg gtgttgttgg tgaaaaatat gtactaagtg
     2101 attattatgc taaccctggt gtttctgacg ttgatcgcaa aaattacgcg caacgctatc
     2161 attgtgctag cgctgaagaa gtaacttaca atcaagaagt cgttaagcaa agtggttata
     2221 caattgtaaa tatttatttg aagcgtacaa cttttaaatg gatgtttaca acaagtgatt
     2281 tttctagaac atataaaact attgaagcaa agtgagatgc caacattcac aatcagttta
     2341 tggaagcgaa tgaagctgct tatggtagca aaatggaatc tgctccatga atttcgaagg
     2401 aaataggaaa ccaaactttc tatgtaaact tacttactaa tatgagtact acaaatcaca
     2461 cgacaaagtc cgaaagctct gataatgccg cttttagaaa ggttgatagt cgctcttcat
     2521 atcgagtaaa tcttggatat atgcttcaaa aaattgatgc cgagacttcg cacaatacaa
     2581 ttaagcgtgt agctaccaat ccttcggatg aggcaaatta tgaggttttt cattcagtaa
     2641 gtgtttgtta ctacagttct tattacggca ctatggtatt ttctaatgac gagtacattc
     2701 caattgaagg ttttacaact ccaacggcag gtacatctga tgggcctgca cttcaaacaa
     2761 atattgcatg gacagacggt cagggcaatt cacacggtta caatttatat tacaaacgaa
     2821 ataaacacga catcagctta aaccgtattg gtggtgctta taattataag aatattgaag
     2881 gtgtttatta tgataaggtt attaatacgg ttttagttcc agaaagttgg gtcaatactg
     2941 aaaaaattgg ttattgggca aatccatctg ctcaaccaat taatacttta gataatttta
     3001 ttccttacaa tccaggcgga aaagttgcag atcagaattt tatttttggt ggctggacta
     3061 tggatcaaac aggccaaggt gatgctgtcg atttaagtca aattaacatg cctaatgctg
     3121 gattaaacct ttatgctaag tgggttgctc cagaagttaa agttacattt gttgaaacag
     3181 atgatccact tcagcctgaa acagaattca aaacaattga ttttgataat gtaacctata
     3241 atcataagct tgaagaatat gctacagctg aaaagcctat tccgcagatc aagaattttt
     3301 atgatgaatc cgaggtcatg acttatgaag gacaagcccg ttattattat gaaaatgatg
     3361 cggcgagaaa agctggctta aagacatatt ggaaccctta tgagatgagt attcctgggc
     3421 ataatattaa ggtttatacc gaatatgcta atgtgaaaac tgttacttat acagtacaat
     3481 acgtacttaa cgtcgatcca actactgaag tagctgctga gcgaactggt gtaggttatg
     3541 agggttcatc tgtagttctc tatccaaaag ttaacaatga gctttatttt gattatcgtg
     3601 ctacatatgt tccaacacaa gataaaatga ctgtaacagt tactgatcag gcagttttca
     3661 cttttaaata tgaagttgct aaagacgtaa acttacgata taacgcttat tacttaaaca
     3721 aggataaact tcctggatca tgagaggaag gaaaacccat tcctccttcg gctatattag
     3781 agcatattac tgaagataaa ctaatggtaa atactgttga tgttgaggca ttagaatttg
     3841 ataatcttac acgtgatgct gatactccag atatacaaac tgttgtttta aatgtcaaca
     3901 atgataataa tgtatttttc tattacaatg aaaatgttgt tgatataaat tacgctgtta
     3961 aaattgatgg tgaagttgtt ccaaaagctg attgaccaac tggtttaaca cttagtaagg
     4021 atattgataa aaatgttaaa gtatttagtg gaatcgcgac ttgtacaatt acgttagatt
     4081 cttcacttac acaatatgag ttctatacat gaacaaagga caacgatgct cgttcattag
     4141 ggaaaaataa tattataaca ttacagtcag gcaaggatcc ttggggaaat gcccctcttt
     4201 cttcgtgcac catttatgca aatttgtcta caaagaatgt aaacctaaag attcaggctg
     4261 gttcaaacgg gcatttagac cctgaacaac ccgaagcagg atgagatgtc ccttttggga
     4321 cctcctatat aattgaaggt gacacaatta catttagtta tggagaagaa agcgataagg
     4381 tgcaagcaat tggtgatcag tatgaaatgc aatggtttac ttttgagtct tggtctatta
     4441 acggttctgg aaaatatgtt tcagccagtg gtacattaaa agaagataca atttttaaag
     4501 cttcctttaa caacactaca ttaaagtgtg aatttaaggt taaagataat cgtggtggag
     4561 aagttctaac ctcacagata tatgttccat ataatgcaaa atgtaagcta gttaatgaca
     4621 gttcattaca attttatgac agtagtgata atccaattgg tgggctaatt tctggaaaag
     4681 aaaatgacca ttttgatttc aagaagtgaa cattagacaa tgaaacaaat ccaatttctt
     4741 ctgaaagctt taccccattg ggtgtctcaa atcatattat ctatgtctga tttgattatg
     4801 aaaaagttaa cttaaaagct gttgttaatg gaagtggtaa tattgatgtt gatggctctt
     4861 ccacgattga ttatactatt gcagttccat acagcacatc ttatagcatg gatgatttat
     4921 ctggaaaaat ctctaatttt gttttacctg aaggttattg aagccaggat gtagacttac
     4981 caaatgcaat tactcctttg cctaatgatg gttggaagtt tgataattga actacaagtc
     5041 ctaagattga aacctataca ttgttattta attcaacgtg cactgcaaat ttctctcaac
     5101 tattatatga tgtttcattt aatgttagtc ctgaagggtc aggttctgtt gaggttgata
     5161 ctccactttt aagtcaagtc ccttatggaa ccacatttgc tcctgatgca actttacctt
     5221 caaagttgat gtttaatgtt gatggtcagc aatgtgctgc ctctgctctt ccttcagagt
     5281 attgatcttt tgttaattgg actgtaaatg gtcaggtagt aacagaaaca aaaacagtca
     5341 ctgacgatac cgtaattgtt gctaattttg ttccagttca gcttacgatt accactgctg
     5401 ttaataatcc ttctcttggc acaattgcac agtttactca taagttttat gctggtacac
     5461 agttatcaat gtcgcaagat ttatctaaag tttcgttgta tgatcaatga actgagcaaa
     5521 ttgaaactac tcagccttca agtattactg agcacgctca cattgacggc tgaactttaa
     5581 atggctttga tatggttgct gaggaaacaa caacaatagg taaggatgcc acacttgttg
     5641 ttaatctttc atatgatgag attacagttc attttggttc atctgatgct cataaaggta
     5701 ttgcatcacg cgaggagggc tcttcggatg gagtggatgt aaaagttcca tattacacaa
     5761 ctttcgttat aaattcacaa aatggatatg ttgatgttaa ggacgctctt aagcaatttg
     5821 taaaagtttt cccacagagt tatgatacag gtgaacaata tttgtttaga aattggacac
     5881 ttaatagcac ggctcttgca acagatacac caattacaat tgatacgcca aatgcacaat
     5941 atttgtgtaa ttttgatctt gtggaatatc cagttagctt tgtaaatgtt tatggcacga
     6001 ccgaggtacc tcgcggaact attgaagtaa aaaagggtgg tgagactgtt gatgatttaa
     6061 ctttaccttg aaacactcct tatcatatta ctcaagatca tagttctgat ccaactcaag
     6121 atagagaatc aaccattact tttggtgatg caagttctaa tgatgttttg gcaacggcta
     6181 aagcaactgc tgttggtcat tatggaggtg aaatatattt ctgagatgac tggtatacct
     6241 cattagttga tgttgaacag cctgatggta ctattactaa agagcatgtt ttttatgctg
     6301 tattttggag agctggtgat cagcgaggta aggttaatca ctatgttcaa cctgtagatg
     6361 aagatggaca attagttact gatcctgcaa agtttaccct tcatgatgct gttatgcaaa
     6421 aggtcactct agatactcat gaagatgcag ttaaaaagac gaatgaagat cctgattttg
     6481 aatattattt agttgacaac gaccatgtac ctgtacagca gttttatatt ccaattgagg
     6541 attacgagaa tattccagaa gtaaattatt attatcctcg tcaggaattc aagcttacaa
     6601 ttaattatgt tattaatgat gaatctgctg atgctcctga tgctcccgat ccatatgagg
     6661 gtaatattgc ttggggtgtt aagtatggta ttgcatctcc aacattggag gggtatgttg
     6721 cagacattac agttgcagaa ggtactatgc caactgaaga tcgtgaagtc actgtaacat
     6781 atacaaaata tgttccgcct actcctcctg agcctactcc agaaagcgag gatattaatg
     6841 ctcaaactgg agatagttca cttgggttag ttggaattct cgttgctatt gttgcaattg
     6901 ctggtgttgg tttgtttgca aatcgcagac gttatgatgt tcgtggtaag cacagcattc
     6961 gatagcagat aactaaaaac ttgaaaaagg cttgtctttt ggcaagcctt ttttgtggag
     7021 gaaatgtgaa gcacggaaag aaaaatatca acaaaaaggt gataattccg ctagcggttg
     7081 ttgttgcggt gctcgtcttc ggccttattt atgccagttg tgtaaagctt atcaatttga
     7141 caccttatgc tgctcagcca agcaatttta cggtagccga tgacttcgac atttcaatta
     7201 aagggacatc tattacttac gaagagcctg ccagttctga caacattttg attaacgcaa
     7261 atgtaaaaaa taacagtgaa gaaggttggg atcttgtatc ttgtttttat attaatgcaa
     7321 aacaggatag ttctaaaatg cttgttgagc cagaaattga gcggactgat ggcctcgcga
     7381 acaatacaac tgctcttgga acgtatattg gtgcaggaga aagtgctgaa attcaacttg
     7441 cgtttagact ttcagattac agcaatgtaa cattaacatt ccagggtttt ccttataacg
     7501 aaaaaacttt cgagccggtt gatgttgttt tgcctgttca caagactgtt gaagtttggt
     7561 ggaatgagat ttgcaattgg tgagatgttt tgggcaagca aaatgttgac agcgttaagg
     7621 ttgcgaattt cgacatcccg cttgccgatg gctgatatgc tgaaactcag gggactgtgg
     7681 cttcggtgtt taagaatccg aagttcccga gcgcgcaaat tacgatgggg gctgcctctt
     7741 cgatggaagc gaaggccctg gctgagcaaa ccgcgcaaat gagcaatatc aaagatccag
     7801 ttcaaagctt tgaaatcaag ggaaaaattt attgaggcta cacaaccgat agtggttttg
     7861 ttctttgctt agatggcgaa gacggcctgg tcctgcgtat caacgcacaa aacatcaccc
     7921 tcgacgccgc ccaacccctc ctctaactcg atttccccgg ggccagtact cgatttcccc
     7981 gtggcccaca ctcgatttac tcgtggcatt tactcgattt tttggggtag atggtga
//
LOCUS       DBNCDC010000051         8003 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_4325_length_8003_cov_6.550453, whole genome shotgun sequence.
ACCESSION   DBNCDC010000051 DBNCDC010000000
VERSION     DBNCDC010000051.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 8003)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8003)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8003
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4325_length_8003_cov_6.550453"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            26..187
                     /locus_tag="AC04VQ_04650"
                     /pseudo
     CDS             26..187
                     /locus_tag="AC04VQ_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012797.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0141098 - tRNA
                     (cytidine(34)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
     gene            548..832
                     /locus_tag="AC04VQ_04655"
     CDS             548..832
                     /locus_tag="AC04VQ_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553448.1"
                     /translation="MLVSEIIQLCENPKLVVFDDFKNLDDDRSKMFSEVIDTFLDLNC
                     EVIVNASPFNDGFIKYQNDSLILKSKDLLLSDKEINGEVKTEIERIPIVA"
     gene            857..1669
                     /locus_tag="AC04VQ_04660"
     CDS             857..1669
                     /locus_tag="AC04VQ_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553449.1"
                     /translation="MIKCAISENFTDAILYSLFIIYLFEEESLDVLSGFLSKSDFSLL
                     NDINDEYIYFGINENKDSFSVPHIFTSKLISLFNSDLQTLISIAKCTDRSDFAEKLCS
                     LLEESNKNEKSLEVAKCLGNRVLQIETLTKFNEDSVRNSYLNTACSLFDKINLPKNND
                     GSLITFNALRLAILGNVGDAFRYSYSVLDNKNSTIDNMLLAALIFIRFGNTNEINQAL
                     KIIRHLNNDFKIDTFLNNENLSDEERFKCKVINRLIEVCFAIHSNSKNPFAV"
     gene            2279..2464
                     /locus_tag="AC04VQ_04665"
     CDS             2279..2464
                     /locus_tag="AC04VQ_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553450.1"
                     /translation="MNINSHWSIFRNLFVLENGDCPYLIKSQNSYKLAKSYFSSDLQD
                     LESLCSDLTLGTLDSQA"
     gene            2492..2845
                     /locus_tag="AC04VQ_04670"
     CDS             2492..2845
                     /locus_tag="AC04VQ_04670"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HIO9553451.1"
                     /translation="MVDGTLLPSETENAYIKQKRNEYKNKVVDALVAAAERLIDAGEI
                     HQALWFAHKALSRDDSREDVFITLMKAQLKAGQRTPAIETYFACKKFLDEELGIEPSR
                     QITDLYSRVVKETPA"
     gene            2876..3124
                     /locus_tag="AC04VQ_04675"
     CDS             2876..3124
                     /locus_tag="AC04VQ_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553452.1"
                     /translation="MYKFSKRSTQMIHFNEVFESQLNAKTISIGLASLFGAILIILSI
                     FFSFLHIENSEAQQSGNTITIANSNGQLVTGPANDNCY"
     gene            3749..3937
                     /locus_tag="AC04VQ_04680"
     CDS             3749..3937
                     /locus_tag="AC04VQ_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553453.1"
                     /translation="MWGSGYNDYSSEPIVSNTVLNIQKTDAQTNSGGVTEGSVESFED
                     AYYDIEYRCDEYKNYVGH"
     gene            3941..4345
                     /locus_tag="AC04VQ_04685"
     CDS             3941..4345
                     /locus_tag="AC04VQ_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553454.1"
                     /translation="MKTNEQGVVGISEKFRDNFIEGDEFLEHDGQIMAPLGTYFVHEV
                     KPSPGYLLSEKEYRINVFPDPENEFKCKVTCVDVHNGTYQPQTIDSQTGEVMIHVDEQ
                     VIRGDYKFRKVNTKGQALSGIPFKIISKTTGE"
     gene            4547..4678
                     /locus_tag="AC04VQ_04690"
     CDS             4547..4678
                     /locus_tag="AC04VQ_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553455.1"
                     /translation="MGAFPFDEYIVEEIPVLANSRYDTSDKRTFKIEENDTVVEGAD"
     gene            4828..>5074
                     /locus_tag="AC04VQ_04695"
     CDS             4828..>5074
                     /locus_tag="AC04VQ_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012798049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553456.1"
                     /translation="MSILSKILTAGEGRQMKAYQDMAAYIGTLEADMKKKTDKQLADL
                     TNKFKERLENGEELKAILPEAFAAVREASVRTTGLRHF"
     assembly_gap    5075..5084
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5085..7615
                     /gene="secA"
                     /locus_tag="AC04VQ_04700"
     CDS             <5085..7615
                     /gene="secA"
                     /locus_tag="AC04VQ_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIO9553457.1"
                     /translation="LIGGMALNDGQIAEMKTGEGKTLVSTLAGYLNALPGNNVHIVTV
                     NDYLAERDSVWMGQIYKFLGMKVGLILNGMMPAAKIPAYQADITYGTNSEFGFDYLRD
                     NMVTKADMRVQRGHTFAIVDEVDSILIDEARTPLIISGAGTKAADTYKSFARAVVGLT
                     PGADFEMDEAKHTIFTTEVGLAKVEAATGIEDIYSDPSGQLANHLQQALRAQFLFHRD
                     DEYVVVDGEVKIVDEFTGRIMEGRRYSEGLHQAIEAKEGVAIKEENQTLATITLQNYF
                     RLYEKLSGMTGTAMTEDAEFREIYKLPVVAIPPNKPVIRDDKNDLVYRNVDAKFYAVA
                     ADIKERHENGQPCLVGTVSIENSEKLSRLLDKQGIKHNTLNAKNHAFEAEIVAQAGRA
                     GAVTIATNMAGRGTDILLGGNPEGMALSFLREDGFDLNADPEDEEAKIPTDKDREDAL
                     ARAKEITESEREIVLNAGGLAVIGTERHESRRIDNQLRGRSGRQGDPGVTQFYLSLED
                     DLMRLFGGPRMESIANMMAKNDIPEDTPIQAGMVSKAIESAQRQVESMHFGSRKHVLE
                     YDDVMNLQRKAIYDERNDILDGKDLGERVTEMFEDAVERIMSEYCPDKSTADEWDIKN
                     VNLWVANLTGRNDFDLASISHDGDVEIAAQALLAYLQTVYDEKCEELSEPIVKVLQPQ
                     IMLRILDTRWMAHLQEMDYLKQGIGLRAYGQLDPLVEYKQEAYSAFKNLTESMYEDFL
                     RTFLRLQMAVEQPLPEQKSALSNNVNYSNPEENLDAKERNAAPAPARAASNAKGMPKQ
                     APKEEIKARTYVKDKNDPYANVGRNDPCPCGSGKKFKKCHGANL"
     gene            7810..>8003
                     /gene="mihF"
                     /locus_tag="AC04VQ_04705"
     CDS             7810..>8003
                     /gene="mihF"
                     /locus_tag="AC04VQ_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HIO9553458.1"
                     /translation="MATPPQLSPEDRAKALAKAKEARTKRAQLRKEIASGKKDLKAVL
                     NSANDPIVGRMKVKTLIEALP"
BASE COUNT         2545 a         1275 c         1706 g         2467 t
ORIGIN      
        1 cgaaaattca tgattagttt ttggaagaga aactgcaggt cttcctccaa agcttattga
       61 agataacaag gacaattgcc ttcgaatccc gatgaagcct gggcttcgga gtttgaacct
      121 ttcaaatgct gttgcagttg ctgcttacga ggctttagga caaacctctg acctggattt
      181 actataatat attaagactt ttacggtcat ttctcaattc tgagtaatgc ttaattaaat
      241 aatttgtata ttagccatcc aaaattttta ggctattttg aaaggttttt ggatggcaag
      301 ttttttatcc aaatgtgcat taagaggctc aagaccaaag aaggtcgagt taaaaaataa
      361 gtgtgtcagg gaagaattga tatcaaaatt gatgtctcaa cgctctgttc ttcgttttat
      421 aagcgcgcca aatggttttg gaaaatctac tttgtgtgct caatatgccg aattagtttt
      481 ttctttctat aaagtatttt gaatagattg ttcatccccg tgttttatta gagatttaga
      541 ttctggcgtg cttgtttctg aaattattca attatgtgaa aatcctaagt tggttgtttt
      601 tgatgacttt aaaaatcttg atgatgatag atctaagatg ttttccgaag ttatagacac
      661 ttttcttgac ctcaattgcg aggttatagt taatgcgtct ccctttaatg atggctttat
      721 taaatatcaa aacgattctt taattttaaa atcaaaagat ttattgctta gtgataaaga
      781 gattaatggg gaagtcaaaa ctgaaattga aaggatcccc attgttgctt gaaaagataa
      841 ttattcggtc agctctttga tcaaatgcgc gatttcagaa aattttactg atgctatttt
      901 atattcgcta tttatcattt atttattcga agaagaaagc ttagatgtct tatccggctt
      961 tttatctaaa agtgatttca gtttgttaaa cgacatcaat gatgaatata tttattttgg
     1021 aattaacgaa aacaaggaca gcttttcggt acctcatatt tttacctcaa aactaatttc
     1081 attgtttaac tccgatttgc aaactttaat tagtattgca aaatgcacag atcgttcaga
     1141 ttttgccgaa aagctgtgtt ctctgttgga agaatctaat aaaaatgaaa aatcattaga
     1201 agttgcaaaa tgtttaggga atagggtttt gcaaatagaa actcttacta agtttaacga
     1261 agacagtgtt cgtaattcat atttaaacac tgcttgtagc ttgtttgata agattaactt
     1321 gccgaaaaat aatgacgggt cgctaatcac tttcaatgct cttcgtttgg caattcttgg
     1381 taatgttggc gatgcgtttc gttattcata tagtgtgtta gacaacaaaa attcaactat
     1441 agataatatg ttgttggcgg ctttaatttt tattcgtttt ggaaatacaa acgagattaa
     1501 tcaagcttta aaaattatca gacacttaaa taacgatttt aaaattgaca cttttctaaa
     1561 taacgaaaat ttaagtgacg aggaaagatt taaatgcaag gtaattaata gacttataga
     1621 agtctgtttt gcaattcatt cgaattctaa gaatcctttt gctgtttgaa attctaatat
     1681 tgaagacgaa ctgtgtttag aggatattat ttgtgctgaa cttattttat attcccatag
     1741 gcacattaaa agaacctttt ttgcttcgga tgatttttca acctttatga ctacactctc
     1801 caggcaattt aatcttgatt caaaattcga ctttaatttt tatacgttcg cattgatcaa
     1861 cacttatcta aatgaatatg aggaattgat aaattgttca actgcaccct tgtgaaaagt
     1921 aaatgaatat ctcaaaagtg aatatcttga ttataaaaaa cagttttcta acgaaaataa
     1981 agaatcagga aaatattctt cgctaagtag tgttggatat tccaacaggc tgcacgcaaa
     2041 tattgataat agtttagttg atgcgatgtt tccttggaat aaagtcccaa agctaaggat
     2101 caatatcttc ggaggcctaa gcgcttttat tggtgataga gaaattatta gcaccaattt
     2161 tggaaggcaa aacataaaac ttatatgttg tattttggct cttgaaaatg ggaatgaaat
     2221 tggcaaaatt gaattggcta agctaatttg accagacagt gatgaagaat gttgcagggt
     2281 gaatattaat tcgcactggt ctatatttag aaatttgttt gttcttgaaa atggtgattg
     2341 tccatatctt ataaaaagtc aaaatagtta taaattagct aagtcttatt ttagtagcga
     2401 cttgcaagat cttgaaagtt tgtgttcaga tttaacttta ggaactcttg attcgcaagc
     2461 atgatgagag gcaattgaga aaaatagaaa tattgtcgat ggaacactcc ttccaagcga
     2521 aactgaaaat gcttatataa aacaaaaaag aaacgaatat aaaaataagg ttgttgatgc
     2581 cttggttgct gcagcagaaa ggttgattga tgcaggagaa attcatcaag ctttgtggtt
     2641 tgctcacaag gcattgtcaa gagatgattc aagagaagac gtttttataa ctttaatgaa
     2701 ggctcagtta aaggcgggtc aacgcacgcc tgcaatagaa acctattttg cttgtaaaaa
     2761 attcttagac gaagaattgg ggattgagcc ttcaagacaa attacagatt tgtattcaag
     2821 ggtggtgaaa gaaacacccg cttaaaaata taaatgtaca acgaaaggag taaaaatgta
     2881 caaatttagt aaacgcagca cacaaatgat tcactttaac gaagtttttg aaagtcagct
     2941 taatgctaaa actatcagta ttggattagc cagccttttt ggggcgattt taattattct
     3001 ttcaatattc ttttcatttt tacatattga aaatagcgaa gcgcaacaaa gcggaaacac
     3061 aataactatt gcaaactcaa atgggcaact ggttacaggt ccagcaaatg ataattgtta
     3121 ttgacattgt tcgcccattg acagtgaggc ttgttataag tcatggtcag gttcaactcc
     3181 ttatgaatac acaatttgtg ttgagacttt tgtaggatct gttgagggga cagttaaaag
     3241 tagtgacttt actaaatttg aacctgggca tcatgaaata aggggttttg atgagggtgt
     3301 cgaatatgat aattgtggat tttgttctgc aaattatgaa ggatgttgag ttgaagatgg
     3361 aacagcatat caccattgaa ctgtaacaag caccaattat tatgatacgt atcaagattg
     3421 aagcggaagc attaaaacaa tagcttatgg tggaattgtt gctgtaaatt attctctttg
     3481 aagttggaca gaaagatgca gagaatattc atttgatggt tttacttttt atgtttataa
     3541 aacttatgag gatgcagaat atagacgaaa tcctgtaggg aatattgtct taggagttaa
     3601 tggtttagga gtttcagagt ttgtatatga agctgaaaaa acatattatg ttcgaccaag
     3661 ctcaatacca ggagaacatg gacatgggta tttatgcaag gacgatatag gacaagttga
     3721 tgttattgct tctgaatacg caaatgtttt gtggggtagt ggttataatg attattcgag
     3781 tgaaccaatt gtatctaaca ctgtgctaaa tattcaaaaa accgatgccc aaacaaatag
     3841 tggcggagtt acagaaggtt ctgttgaatc ttttgaagat gcatattacg acatagagta
     3901 tagatgtgat gaatataaga attatgtcgg ccactgagtt atgaaaacta acgaacaggg
     3961 cgttgttgga ataagtgaaa agtttagaga taattttata gaaggggatg aattccttga
     4021 acatgacgga caaataatgg cccctttggg aacgtatttt gttcatgaag ttaagccttc
     4081 tcctggttat ttacttagcg aaaaagaata tagaattaat gtttttcctg accctgaaaa
     4141 tgagtttaaa tgtaaagtta catgtgttga tgttcataac ggaacttatc agcctcaaac
     4201 tatagattct caaactggcg aagttatgat ccatgttgat gagcaagtta taaggggcga
     4261 ttataaattc cgaaaagtaa atacaaaagg tcaagcatta tctgggattc cttttaaaat
     4321 tatttctaaa acaacaggcg aatgacatat ttgtgtaagt gacgatgacg gaataatcga
     4381 cacaagcact tcgttcgatt caagaacttc attaacaaat tgcaatgatt atctttatga
     4441 cgaagaaaca aattcaatta ccaacgaaga tttgttaaca agtaattgtg gagtttgatt
     4501 tgcctacgat aagaaaaata atcgtgttgc atctgccaag gacaacatgg gtgcctttcc
     4561 ctttgatgaa tatatagttg aggaaatacc tgttcttgct aacagcagat atgacacatc
     4621 ggataagcga acttttaaaa tcgaagaaaa tgacacggtt gttgaaggag ctgactgaat
     4681 taataaagaa cgcaaaaatc caattaatcc tgttaatcca gtcagtccaa caagtcctgc
     4741 aagcaacgca actcccaaaa ctggcgatac tcctttatac atatttggaa tatctattct
     4801 tgctgtttta tgttatatta tgcgccgatg agtatactgt cgaaaatatt aactgctggt
     4861 gaaggtaggc agatgaaggc ctaccaggat atggccgcat atattggcac attagaagcc
     4921 gatatgaaaa aaaagaccga taagcagctt gccgacctaa ccaataagtt taaggaaagg
     4981 cttgaaaacg gtgaagagtt aaaggcaatt ttgcctgaag ctttcgcagc ggttagggaa
     5041 gcgagtgttc gcacgactgg gcttcggcac tttgnnnnnn nnnnagctaa ttggtgggat
     5101 ggctctaaat gatgggcaaa ttgctgagat gaaaactggt gaaggtaaga cactcgtttc
     5161 aactttggca ggctacttaa atgcccttcc aggaaataac gttcatattg ttacagttaa
     5221 tgattactta gccgagcgcg atagtgtttg gatgggtcaa atttacaagt ttttgggaat
     5281 gaaggttggc cttatcctaa atggtatgat gccggctgct aaaattcctg catatcaagc
     5341 tgatattaca tatggaacaa acagtgaatt tggctttgat tatcttcgtg acaacatggt
     5401 tacaaaagcg gatatgagag ttcagcgtgg acacactttt gctatcgtcg atgaggttga
     5461 ctcaatcttg atcgacgaag caagaacacc actcattatt tctggtgctg gaactaaggc
     5521 agcagataca tataagagtt ttgcacgtgc agttgtcggg cttactcctg gtgctgattt
     5581 tgaaatggac gaagccaagc acactatatt tactaccgaa gttggtcttg ctaaggttga
     5641 ggctgcaact ggaattgaag atatctattc ggatccgtca ggccagttgg cgaaccactt
     5701 gcagcaagct cttcgtgctc aatttttatt ccacagagat gatgaatacg ttgttgtgga
     5761 tggagaagtc aaaattgttg atgagtttac tggtcgtatt atggaaggtc gccgttattc
     5821 cgaaggtttg catcaagcta tagaggctaa ggaaggcgtt gctattaaag aggagaatca
     5881 gactcttgca acaatcacac ttcaaaatta cttccgcctt tatgagaagc tttctggcat
     5941 gacaggtact gctatgacag aggatgctga gtttcgtgag atttataaat tgccagttgt
     6001 tgcaattcct ccaaacaagc ctgttatccg tgatgacaaa aatgatttag tttatagaaa
     6061 tgtagatgcg aagttttatg cagttgctgc cgatattaaa gagcgccatg aaaatgggca
     6121 accttgcttg gttggtactg tttcgattga aaattctgag aagttatctc gtttgctgga
     6181 caagcaaggt atcaagcaca atacattaaa tgctaaaaac catgcttttg aagctgagat
     6241 tgttgctcag gcaggtcgtg caggtgctgt aacaattgct actaacatgg ctggtcgtgg
     6301 tactgatatt ttgctcggtg gtaatcctga aggaatggcg ctatctttcc ttcgtgagga
     6361 tggctttgac ttaaatgccg accccgaaga cgaagaagcc aagattccaa ctgataaaga
     6421 tagggaagat gctcttgctc gtgcgaagga aattacggaa agcgagcgtg agattgtgct
     6481 taatgctggt ggccttgcag taattggtac agagcgtcat gaatctcgtc gtattgacaa
     6541 ccagcttcgt ggtcgttctg gtaggcaggg ggatcctggt gtcactcagt tctacctttc
     6601 attagaggat gacttgatgc gtctcttcgg tgggcctcgt atggaaagca tcgctaatat
     6661 gatggcgaaa aatgatatcc ccgaagacac tccaatccag gcgggcatgg tttccaaagc
     6721 tatcgagagc gcgcaacgtc aagttgaaag catgcacttc ggttccagaa agcacgtttt
     6781 ggaatacgac gatgttatga atcttcaacg taaagcaatc tatgatgagc gtaatgatat
     6841 tttggatggc aaggatttag gcgagcgcgt aactgaaatg tttgaggatg cggttgaacg
     6901 cattatgagc gaatattgcc ctgataaatc aactgctgat gaatgggata ttaagaatgt
     6961 taatttgtgg gtcgcaaacc tgaccggccg aaatgacttt gacctggctt cgatttctca
     7021 cgatggtgat gtagaaattg ccgctcaagc attgcttgct tacttgcaaa ctgtttatga
     7081 tgaaaaatgc gaagagcttt ctgagccaat tgtaaaagtt ttacagccgc aaattatgct
     7141 tcgtattttg gatactcgat ggatggcgca tcttcaagaa atggattact tgaagcaagg
     7201 tattggactt cgtgcttatg gtcagctgga tcctcttgtt gaatacaagc aagaggcata
     7261 tagcgctttt aagaatttga cggaaagtat gtacgaagat ttcttgcgaa ccttcctgcg
     7321 tttgcaaatg gctgttgagc aaccgctgcc agaacaaaaa tctgctttat ctaataatgt
     7381 taattattca aacccagaag agaatttgga tgcgaaggaa cgcaatgcgg ctccggctcc
     7441 tgcgagggca gcatcaaatg cgaagggtat gccaaagcaa gctccgaagg aagagattaa
     7501 agctcgcaca tatgttaagg ataaaaatga tccttatgca aacgttggtc gaaacgatcc
     7561 atgcccatgc ggctctggca aaaaattcaa aaaatgccac ggcgccaatt tgtaagaaat
     7621 tatgttcgga attgtattat ctaccaggat ttaagcttcg tttaatatta aaaccccagg
     7681 tcagaggctt ggtataaaaa aattacattt ttcctgcaaa taggctattt tttgtgtata
     7741 atacgagcgt acacgatatg tgtacaaatg ttaggcggga cttctgtccc aagagaaaag
     7801 gagattagta tggctacacc accacaacta tcaccagagg atcgcgctaa ggctcttgct
     7861 aaggctaaag aagctcgtac aaagagagct caacttcgca aggaaattgc ttctggcaaa
     7921 aaagacctta aggctgttct aaacagtgct aacgatccta ttgttggtcg tatgaaggtt
     7981 aagactctta tcgaagctct tcc
//
LOCUS       DBNCDC010000052         7927 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_4407_length_7927_cov_6.755716, whole genome shotgun sequence.
ACCESSION   DBNCDC010000052 DBNCDC010000000
VERSION     DBNCDC010000052.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 7927)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7927)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7927
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4407_length_7927_cov_6.755716"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="AC04VQ_04710"
     CDS             <1..1063
                     /locus_tag="AC04VQ_04710"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIO9553459.1"
                     /translation="KMFTLIIGWITFLALDAPALSVMSLAIVNYLQFFFKVDPFLLKF
                     VAIVIIFGFACMHIRSVKVGSTVQALITLIKIVPFVLIIGVGAFFINPDLLLSPEPIA
                     HASNMVPQGFSLMPLFSAIALSFFSCDGVFAGCYVSGEIKNPKRTLPLGLVLSVLIIM
                     LLYVGLTTTATGLMSVDEIASSTAPIADMAGKIPVIGIYAGPIIAVVAIIVIMGTISS
                     CMLYMPRFEFAMARDGLFFPIFAKVHRKFKTPHRAITIFCAYAIVLVFFGDLSSLLGA
                     LTIIILLKNGLTFATIFILRRKEGYNPTYKAPGNWVMPVISVLGTGCLLIFAIATATP
                     FQLLFNGGIILLGIAAYFI"
     gene            complement(1082..2719)
                     /gene="priA"
                     /locus_tag="AC04VQ_04715"
                     /pseudo
     CDS             complement(1082..2719)
                     /gene="priA"
                     /locus_tag="AC04VQ_04715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication restart helicase PriA"
     gene            complement(2822..3211)
                     /locus_tag="AC04VQ_04720"
     CDS             complement(2822..3211)
                     /locus_tag="AC04VQ_04720"
                     /inference="COORDINATES: protein motif:HMM:NF036997.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N' family DNA-binding
                     protein"
                     /protein_id="HIO9553460.1"
                     /translation="MQVAKVILDIQSNQFSKPLDFAILPGKSPQVEFDSKENYEAEVG
                     CVVVVPFGKQYKLGFIINIVNESDISRPANTLKAIACVASDSFFDSTQVEFAKFVSEK
                     YLCSFATAMRLFIPSGSMPKLKHVGDK"
     gene            complement(3326..3730)
                     /locus_tag="AC04VQ_04725"
     CDS             complement(3326..3730)
                     /locus_tag="AC04VQ_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIO9553461.1"
                     /translation="MFNSFYLGFSIATAPLMSYQFGAKNKTEMRNLLKCCLTIIVVAS
                     VICFALSEVLAPAFAGIFVSYDEQLAQYSVYAFRIYSILFLFAGISIYGSSLFTSLGD
                     GLTSALIAFLRTLVFEVAALLILPEIIGSDGI"
     gene            complement(3734..4528)
                     /locus_tag="AC04VQ_04730"
     CDS             complement(3734..4528)
                     /locus_tag="AC04VQ_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIO9553462.1"
                     /translation="MMFTISVYVIIDGFFISNFVNSTAFAAVNLIFPVIMIFTSFGMM
                     LATGSSAIIAKKLGENHPNTANRYFSMFVYFIIIGGIVLSIIGIFIMRPVALLLGAEG
                     ELLEIAVYYGLMSLISLPAYILQTAFTTFFNTAGKPRMGLITAIISGVAIFLLDLILV
                     AFLKFGINGILIATITSEYISAIIPLSYFARKNNKSLLRLVGFREAFRNQDPSTLSLI
                     IKSSLNGSSEAVQETAASLVILLCMFQINRFIGEDGLVAYGIADYA"
     gene            5056..6372
                     /gene="rpoD"
                     /locus_tag="AC04VQ_04735"
     CDS             5056..6372
                     /gene="rpoD"
                     /locus_tag="AC04VQ_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIO9553463.1"
                     /translation="MEFDGKKTAKKSTKKSSKKVEAYDADALDKELSAVAAEPIEEPE
                     EVDITETLKDSDIDKQVAAEEKKLAEDAEDGEEAIDSEDILEGIPESELNAVPEPGAL
                     PRVSNKNTRSARVRARKHVFDQSTVMLMGDPVRMYLKEIGRVPLLDAEQEKALAMKIE
                     NGVKASKELDALYEKEKAGKKVDRKEKRRLSRKEQVGIDAKSDLTEANLRLVVSIAKR
                     YIGRGMTFLDLIQEGNLGLIRAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIR
                     IPVHMVETINKMTRIQRQLLQEKGREPTAEEIGELMDLPADRIREIQRISQEPVSLET
                     PIGEEEDSQLSDFIEDEKAVMPIDAASFSLLQDQLMKVLGSLGERERKVITLRFGLQD
                     GLPHTLEEVGNEFNVTRERIRQIESKTLAKLRHPSRSSKLRNYLTD"
     gene            complement(6373..6738)
                     /locus_tag="AC04VQ_04740"
     CDS             complement(6373..6738)
                     /locus_tag="AC04VQ_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553464.1"
                     /translation="MSCVFYCESERKHSAEFLFLAYPKKHAEAYENFIETVSSKLTEG
                     NRPDINLDEFECKEIEKSNGNFKITKFLAKPKLEKGTVILKNKQGLMEKIIEAGRQKK
                     IGCYIGSILFLLVIIGLIV"
     gene            complement(6769..6975)
                     /locus_tag="AC04VQ_04745"
     CDS             complement(6769..6975)
                     /locus_tag="AC04VQ_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553465.1"
                     /translation="MMQKNNAIYTIANPKDKRQAAQLTSSNSLCGNRFSLKVNSKNPV
                     VTIKNKFDYAVAELSEEDSKLIKL"
     gene            7008..7271
                     /locus_tag="AC04VQ_04750"
     CDS             7008..7271
                     /locus_tag="AC04VQ_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553466.1"
                     /translation="MIQEISNILRKTKELKPYYKKNEAGEDASLAVVSSARPLVVATD
                     FIANKKSMLVVVAGDKNAKVFANTINSYIPGSGVRLAGNMEAL"
     gene            7275..>7927
                     /locus_tag="AC04VQ_04755"
     CDS             7275..>7927
                     /locus_tag="AC04VQ_04755"
                     /inference="COORDINATES: protein motif:HMM:NF037626.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553467.1"
                     /translation="MHSGKAKIVVASASEILRKIPDTDIAKLKPIRVVKDKECDYKSL
                     MDKLKSFGYTRLSVLDGQGTFSVKGGTVDIFPSQLNYPVRIDFFGDEVEDIRRIVQST
                     GQTINSLKEVEIFSAKQSEDFEKTVPLQDLISIKTVVYMDEPRGVLDDMRNYYASLGK
                     SIKMSRADRKTFIMDPLKVSFSGNQQISLVSIMQRGINPDAKLTLKRPPKHKTIEELR
                     "
BASE COUNT         2399 a         1436 c         1589 g         2503 t
ORIGIN      
        1 taagatgttt actctcatta ttggttggat tacgtttctt gctcttgatg ctccagcatt
       61 atcagtaatg tctttagcaa ttgtcaatta tctgcaattc ttttttaagg ttgatccatt
      121 cttattaaaa tttgtggcaa ttgtaattat ttttggattt gcatgtatgc atatacgatc
      181 tgttaaggtt gggagcactg ttcaggcatt aatcactctt attaaaattg ttccatttgt
      241 tttaattatt ggtgtaggtg cattttttat taatccagat ttattattgt ctccagagcc
      301 tatagcgcat gcaagtaaca tggtgcctca aggatttagt ctgatgcctt tatttagcgc
      361 cattgcgctt agttttttct cttgcgatgg tgtatttgct ggctgctatg tttcaggaga
      421 aattaaaaac cctaaaagaa ctcttccttt agggcttgtt ttgtcagtgc tgataattat
      481 gcttttgtat gttggattaa ctaccaccgc taccggactt atgtcagttg acgaaattgc
      541 ttcaagtaca gcccctattg ctgatatggc tggtaaaatt cctgtaattg gtatttatgc
      601 tgggccaatt attgcagttg ttgctattat tgttattatg ggaacaattt caagttgtat
      661 gctttatatg ccacgctttg aatttgcaat ggctcgagac ggactcttct tcccaatatt
      721 tgcgaaggtg cataggaagt ttaagacacc tcacagagca ataactattt tttgtgctta
      781 cgctattgtg ctagtgttct ttggagattt gagcagtctc cttggtgcgt taacaattat
      841 tattctctta aaaaatggtc ttacatttgc aacaattttt attctccgaa gaaaagaagg
      901 ctacaatcct acttataagg ctccaggcaa ttgggtaatg cctgtaatat cagttttagg
      961 tacaggatgt ttgcttatat ttgctattgc tacagctact ccgtttcagc ttcttttcaa
     1021 tggaggaatc atcctgcttg gcatagcggc ctatttcatc tgaaaacatt tcaacaagta
     1081 attacaataa gttgataggg tcgatgtcaa ttgtgacttt gacttttgca gttttcttta
     1141 aaggctttaa gcattcttct atgtattttg acaagtcaaa attttgcggc gcctttaata
     1201 ctatatggaa tcgatactgg tttcttatct tttcaagaac acagcttgta gaaggcatta
     1261 tgttaacgtc agaaggtaag tctttttcaa gaagctcttg tatattctta ctatgttgca
     1321 aagcagcatc ttcgtttgtt gatgataaga caatgtttgc aagttgataa aatggaggga
     1381 agtgtaagag ttgacgcttt tgcgcttcta ttgacataaa tgtgtctttg tcatatatgc
     1441 aagcaagctc aatagcttcg ctatctggcg tatatgtctg gacaatcact ttacccggct
     1501 tttcacctcg tccagcacgc cctgcaactt gcaaaatcat attaaatgtt ctctccgaag
     1561 atctaaaatc cggaagagtt aaatttgtat caacaaggac aattcctacc aaggttacat
     1621 cattgaagtc aaggcccttc gcaatcattt gagtgccaag aagcacagca gggccaggct
     1681 ttgaaaattc gtccaaacac ttttgatgac ctccaacatt ttttgttgtg tctgcatcca
     1741 ttcgaataat tcggacgttt ccaagattgt tttcatcaag aagggcactc aacgcatctt
     1801 cgacattttg tgttcccgca ccgtatcttg ctatgtaagg gctgccgcat tctggacatt
     1861 ttacaggcac tgcttctttg tgaccgcaat gatgacattt aagttcttgc gtagttgatg
     1921 ttgtgttgtg gcttcggtgg taagtcattg tggtagaaca attagtgcaa gttggggtat
     1981 agccgcaatt tctgcaaaac atgtagtttg aaaaacctcg tctgttatga aagagcagaa
     2041 ccttgtttcc atcattgagt tcttgaataa tgttttttgt tagcgcaaat gaaaaaagac
     2101 tctttcgacc gctcttaaac tcttttccca tatcaacaat ttcaatatta ggaagaggct
     2161 tgccgtttgt ccgctctgga agttttatta aatgccaatc agcgaagttc tggcagttgt
     2221 gcaatgcctc catcgaaggt gttgcagagc ccaaaacaag tacagagttg catttctttg
     2281 ctaaccaaga agcgcaatca cgtgtgtgat accttggatt gttatcttgt ttatatgaat
     2341 tctcgtgttc ttcatccatg acaattagac cgatattttg caagggacaa aatagtgcac
     2401 tacgggcacc aataacaagt ttaacctgcc cgttttttat tcgataaaac tgattgaatc
     2461 gctcagctgg tgtcattttt gaatgcaaaa ctgcaatttt attgccaaag cgagcgctaa
     2521 atctagaaac ggtttgaggc gtaagtgaaa tttctggaac cagtacaatc gccctttttc
     2581 ctttttcaaa aactttatta attgcacgaa ggtaaacctc tgttttgcca ctgcctgtaa
     2641 ctccatcaag cacaaatgtg ttatttgatt ctgaatcaat cgccgaagta atagcatcaa
     2701 atgcttcgcg ttgtcctttt gttagttcga agttttctca gttgctctct tcgaaatttt
     2761 ttgaaatata tttttccgtg taatttttat cactaattct gaaattgtga gtagtcgcaa
     2821 ttcatttgtc gccaacatgc tttagcttag gcattgatcc gcttggaata aatagacgca
     2881 tagcagttgc gaaggaacat aaatattttt cggacacaaa ttttgcaaat tcaacttgtg
     2941 tcgagtcaaa gaacgaatcg cttgcaacac acgctattgc ttttaatgtg tttgccggac
     3001 gagaaatgtc tgattcattg actatattta tgataaagcc aagtttatat tgctttccaa
     3061 aggggactac aacaacgcag cctacctcgg cttcgtaatt ttctttagaa tcgaattcaa
     3121 cttggggact tttcccaggt aaaatagcga aatcaagagg tttcgagaac tggttgcttt
     3181 gtatgtctaa aattactttt gcaacttgca tggtagcgct attctaatac attttattta
     3241 attagtgaaa gaactttttg tattttgtgc aaataaaaat aatagttagt attgctgcga
     3301 ggaattctgc aattccgact gaatatcaaa ttccatcaga accgattatt tctggcaaga
     3361 ttaacaatgc ggctacctca aaaactaatg tacgtaagaa cgctattaac gccgaagtca
     3421 agccgtcgcc caaacttgta aatagggaag atccgtaaat ggaaatacca gcaaacaaga
     3481 ataagatcga atatattcta aatgcataca cagaatattg tgccagttgt tcgtcgtagc
     3541 taacaaagat accggcaaaa gcaggagcta gtacttcgga taaggcgaag caaattactg
     3601 aagcaacaac aataattgta aggcaacatt taagtaagtt gcgcatttca gttttgtttt
     3661 ttgctccgaa ttgatagctc attagtggag cagttgctat tgaaaatcct aaatagaacg
     3721 aattgaaaat aactcatgcg tagtctgcga tgccgtatgc aactaatcca tcttcgccaa
     3781 taaatctatt aatttgaaac atgcacagca atattactaa cgatgcagca gtttcttgaa
     3841 cagcttcgct agatccattt aatgaagatt ttatgattag ggatagcgtg cttggatctt
     3901 ggtttctaaa agcttctcta aaacctacta agcgaagtag gcttttattg ttcttgcgtg
     3961 caaagtaaga cagtggaata attgcactta tatattccga agtgatagtc gcaattaata
     4021 ttccgttgat tccaaatttt aaaaatgcca ccaatattaa atctaataaa aatattgcaa
     4081 cgccagatat aatcgcagtt attagtccca ttcttggttt gcctgcagta ttaaaaaagg
     4141 ttgtaaatgc agtttgaaga atgtaagctg gaagcgaaat taaagacatt aacccataat
     4201 aaaccgcaat ttctagaagt tcgccttcgg cccctaaaag aagtgctact ggtctcatta
     4261 taaatattcc gataatcgac aaaacaattc cgccaataat tatgaaataa acaaacatag
     4321 agaaatacct gtttgctgta tttggatggt tttctcctaa cttctttgcg ataattgcac
     4381 tagagccagt tgccaacatc atcccaaaag aagtaaatat cataattaca ggaaagataa
     4441 gatttactgc tgcaaaggct gtagaattca cgaaattact aataaagaat ccgtcgataa
     4501 ttacataaac agatattgta aacatcatta caatagaagg caacgtatac tttatcagct
     4561 gaaattttgt tcaatgctta tttaatgttg tctgtttttg actcatacat ttatcagttt
     4621 aacataatat tttttaaatt gaaataagta taaagttttg gttagtgcaa ttcgtggttt
     4681 ataaaattta attttgtggt ataaataata gcttggtaat tcaatagaaa ggattaaatg
     4741 gctactaaag ctaagacatc taaaactgtg aaaaaatcac caaaaaagac cactacaaaa
     4801 gcttctgcta agaaaactgc ggcaagtgcg ccgaaagcca aaaaggcaac tacaaaaaag
     4861 caagctaaac cagtggcaaa gaaaacagta gcgaagaaaa caacggcaaa aaaaccagca
     4921 aagaaaacaa cggcaaaaaa gactacagct aaaaaagcga ctccaaaaaa ggctagcgcg
     4981 aagaaaactt cggcagctgc tcaagcaaaa agaaatgctc aacttgcaaa agaaatgcaa
     5041 gagctttttg atgctatgga gtttgatggc aagaagactg ctaagaagtc aacaaaaaaa
     5101 agttctaaaa aggttgaagc atatgatgcg gatgctcttg ataaggaact ttcagcggtt
     5161 gcggctgaac ctattgaaga acctgaagag gttgacatta ctgagacctt gaaggattca
     5221 gatatcgaca agcaagttgc ggccgaagaa aagaaacttg ctgaagatgc tgaagatggc
     5281 gaagaggcaa ttgattcaga agatatttta gaaggtattc cagaaagtga attaaatgct
     5341 gttcctgagc caggcgcgct tcctcgtgtt tccaacaaaa atacacgttc tgctcgtgtt
     5401 cgtgcacgta agcatgtctt tgatcaaagc actgttatgc ttatgggcga cccagttcgt
     5461 atgtatctta aagaaatcgg tcgtgttccg cttcttgatg ctgagcaaga aaaggccttg
     5521 gctatgaaga ttgaaaatgg cgtaaaggct tctaaggaac ttgatgctct ttatgaaaag
     5581 gagaaggctg gcaagaaagt tgatcgtaaa gaaaagcgtc gccttagccg taaagagcaa
     5641 gttggtattg atgccaagag tgaccttaca gaagctaacc ttcgtcttgt agtttcaatt
     5701 gcgaagcgct atattggacg tggtatgact ttcttggatt tgattcagga aggaaacctt
     5761 ggacttatcc gtgcggttga aaagtttgat taccgcaagg gtttcaaatt ttcgacttat
     5821 gcaacttggt ggattcgcca ggcaattact cgtgctattg ctgatcaggc tcgtacgatt
     5881 cgtattcctg ttcatatggt tgagacaatt aataagatga ctcgcattca gcgtcaactt
     5941 ttgcaggaaa aaggtcgtga gcccacggca gaagaaattg gtgagctgat ggatcttccg
     6001 gccgaccgca ttcgtgaaat tcaacgaatt tcgcaagagc cagtttcttt ggaaactcca
     6061 atcggcgaag aagaggacag ccagctttca gactttatcg aagatgaaaa agccgtcatg
     6121 ccaattgatg cggcaagctt ttcgcttttg caagaccaac ttatgaaagt tttgggttct
     6181 ttgggcgagc gcgaacgcaa ggttattacg cttcgttttg gacttcaaga tggacttcca
     6241 cacacgctcg aagaagttgg aaacgaattt aatgtaactc gtgagcgaat taggcaaatt
     6301 gaaagcaaga ccttggcgaa gcttcgtcac ccgtcacgct cttcaaagtt aagaaattat
     6361 ttgactgact aatcaaacga taagtccaat aattactaaa aggaatagga tgctgccaat
     6421 atagcatcct atttttttct ggcgcccggc ctcgatgatt ttttccatta agccttgttt
     6481 atttttaagg atgaccgttc ctttttcaag ttttggtttt gctaaaaact ttgtaatttt
     6541 aaagttcccg ttagatttct caatttcttt acactcaaat tcatccaggt taatatctgg
     6601 tctgttgccc tcagtaagtt ttgatgaaac cgtttcgatg aagttctcat aagcttctgc
     6661 atgttttttt gggtaagcca ggaataaaaa ttcagctgaa tgttttcttt ctgattcaca
     6721 gtaaaataca caactcaaca acgcaacaac ttctaagtct ttgttaattc atagttttat
     6781 aagtttggaa tcttcttcgg aaagttctgc tactgcatag tcaaatttat tttttattgt
     6841 tacaactgga ttttttgagt tgacttttaa agaaaagcga ttaccacata gcgaattaga
     6901 gcttgtcagc tgagctgcct gccgtttgtc ttttggattt gctattgtgt atattgcatt
     6961 atttttttgc atcatagatg caattttatc ataaaatgta aatcgcgatg attcaagaga
     7021 ttagtaacat attaagaaaa actaaagagc taaagcctta ttacaaaaag aacgaggcag
     7081 gcgaagatgc gtcacttgcg gttgtaagtt ctgcgcgacc acttgtagtt gcaacagatt
     7141 ttatcgctaa taaaaaatca atgcttgttg ttgtagcggg tgataaaaac gctaaagttt
     7201 ttgccaatac aatcaactct tatattccgg ggtcgggggt gcgcttagct gggaatatgg
     7261 aagcgttgtg atgcttgcat tctggtaaag ctaaaattgt ggttgcctcg gcttcggaga
     7321 ttttaagaaa aattccagac actgatattg ctaaactaaa gccaatacgt gttgttaagg
     7381 acaaagagtg tgattataaa agcttgatgg ataagctaaa aagttttggc tacacacggt
     7441 taagcgttct tgacgggcag ggaacattta gcgttaaggg cggcacggtt gatatctttc
     7501 cttcgcagct aaattaccct gttagaattg atttttttgg tgacgaagtt gaagacatcc
     7561 gtcgcatcgt tcaaagcacc gggcaaacta ttaattcgct gaaagaagtt gaaatttttt
     7621 ctgcaaagca aagcgaagat tttgaaaaga ctgtcccgct tcaagacctt atttcaataa
     7681 agactgttgt ttatatggat gagcctcggg gcgttctgga cgacatgcga aattattacg
     7741 cgtctcttgg taagtctatt aagatgtcgc gcgcggacag aaaaactttt atcatggacc
     7801 cacttaaagt tagtttttct ggcaatcagc aaatttccct tgtctctatt atgcagcgag
     7861 gaattaatcc ggatgcgaag ttgacgttga agcgtcctcc aaagcacaag accatcgaag
     7921 aacttcg
//
LOCUS       DBNCDC010000053         7905 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_4434_length_7905_cov_6.826369, whole genome shotgun sequence.
ACCESSION   DBNCDC010000053 DBNCDC010000000
VERSION     DBNCDC010000053.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7905)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4434_length_7905_cov_6.826369"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="AC04VQ_04760"
     CDS             <1..245
                     /locus_tag="AC04VQ_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIO9553468.1"
                     /translation="IICRCESISEGEIMDAINRPVGAKSLDGVKRRVRAGMGRCQGGF
                     CSPRVMEILARELKKDITEITKRGGKSKVLLGKNKE"
     gene            242..1507
                     /locus_tag="AC04VQ_04765"
     CDS             242..1507
                     /locus_tag="AC04VQ_04765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006361340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIO9553469.1"
                     /translation="MKIEEFDIVIIGGGPAGLAAAASACEAGCESIVILERDHELGGI
                     LNQCIHNGFGLHTFGEELTGPEYADRFIKKVKDLGIEHRLNTFVLNISEDKQITCMNR
                     TDGLYTINAKAVILAMGCRERSRGALNIPGYRPAGIFSAGTAQRFVNMEGYMPGREVV
                     ILGSGDIGLIMARRMMLEGAKVKVVAEIMPFSGGLKRNIVQCLDDYGIPLKLSHTVVD
                     IEGRERVEGVTIAEVDEHMKPIPGTEEHYSCDTLLLSVGLIPENELSLSAGVELHPVT
                     KGAFVNESLETSVPGIFACGNVLHVHDLVDFVSEEAATAGRNAASFVESTPKKGQNIE
                     VVTQGGVTYCVPSFIDPTKIEDKLTLRMRVNNVYKGKYLSLYFDDERIKHKKRPVMAP
                     GEMESIELTKADFDAASSLKKITIKIEDK"
     gene            1507..1869
                     /locus_tag="AC04VQ_04770"
     CDS             1507..1869
                     /locus_tag="AC04VQ_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006361341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HIO9553470.1"
                     /translation="METKNLTCINCPLGCQVEVKLDGGKVVEVTGNTCKKGEEYAIKE
                     VTNPTRIITSSVIVEGGTGTKSQVSVKTERDIPKDKIFDVMAALAGVSVKAPVKIGDV
                     VLENVAGTGVNIVATRNI"
     gene            complement(2005..2541)
                     /locus_tag="AC04VQ_04775"
     CDS             complement(2005..2541)
                     /locus_tag="AC04VQ_04775"
                     /inference="COORDINATES: protein motif:HMM:NF040368.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6273 domain-containing protein"
                     /protein_id="HIO9553471.1"
                     /translation="MDNLLETYKPIGGTFIIQDLDDENETLAKTKRINLEGIGNYKVR
                     VIAENHDVCENGKTAALTFEFTKVISNVPFAKNNVISNEWESSELRNYLNNKLFEMFP
                     ADLKSSIKTVRKDTCVGGGSDEVKTFNEKVFPLSVTEMNITGINPIVEGKPYKLYATH
                     PYKPGDDDDPRNKDDKSY"
     gene            complement(2572..2700)
                     /locus_tag="AC04VQ_04780"
     CDS             complement(2572..2700)
                     /locus_tag="AC04VQ_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553472.1"
                     /translation="MTNKIGRSTFVITICAIAVAVVFAVSSCGKSTAEPKSFAFDS"
     gene            complement(2711..3352)
                     /locus_tag="AC04VQ_04785"
     CDS             complement(2711..3352)
                     /locus_tag="AC04VQ_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIO9553473.1"
                     /translation="MDKLTSFLNERSASPTSYLKFKNNIGDNIHGDANFAINLVQNNT
                     VKEQLIIFDSNRYRDSTSDKGYSDYDYIYPDQIEWYKQLVTKTAEANSGAVVPSLAFF
                     HIPLQEYLFAYEAVQRGEAKFLDDGRDTEGICCPRENTGLFDVMKELQSTKGVFCGHD
                     HVNHSCIEYQGIKLAYGLKSSDFIYHDEGWLGGRLISLTPNGEFSSKLIHNDY"
     gene            complement(3554..3748)
                     /locus_tag="AC04VQ_04790"
     CDS             complement(3554..3748)
                     /locus_tag="AC04VQ_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553474.1"
                     /translation="MKKKMFAKISILGCLCLCLACGMLACNKNNKPNVNINDYTIDVP
                     YKDNFQVLQLTDTHWLGGTD"
     gene            3855..5003
                     /locus_tag="AC04VQ_04795"
                     /pseudo
     CDS             3855..5003
                     /locus_tag="AC04VQ_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
     gene            4993..5766
                     /locus_tag="AC04VQ_04800"
     CDS             4993..5766
                     /locus_tag="AC04VQ_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013909.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HIO9553475.1"
                     /translation="MKIRELEKLMLEKFPLETAEEWDTTGVTSGDEDKEITRVAVALD
                     PSISAVKEAKKRGCNLLVTHHPAYIGEIAGQPLRNIIFHAPNYGVALMNFHTCLDVSD
                     EGAATLPNLIGLNAKQTLILTCGKLGFGKICTPANGKSTTLSALAKKCESVFGRKPRV
                     WGKASSSLKKIVTTTGSVGRLDDEVSVLQAAIDKRVDCIICGEVKYHDAIEMLERGIC
                     IIELGHDVSELPLCDVLKNSLVDCGIAKIDIIMIDQNNY"
     gene            5785..6030
                     /locus_tag="AC04VQ_04805"
     CDS             5785..6030
                     /locus_tag="AC04VQ_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553476.1"
                     /translation="MKKIKYISGGIFIAICICVLCVLTSCVSSQAPKVSADAQGLHHH
                     DGYTLEQMTVVSRHQIRSALSEHGSDLAKINSHEWLP"
     gene            6061..7110
                     /locus_tag="AC04VQ_04810"
                     /pseudo
     CDS             6061..7110
                     /locus_tag="AC04VQ_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012548.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine-type phosphatase"
     gene            7123..7836
                     /locus_tag="AC04VQ_04815"
     CDS             7123..7836
                     /locus_tag="AC04VQ_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIO9553477.1"
                     /translation="MAGKVEDIEKLLRLQEVDNTIEDGAEAFNNSPIATEIGKVRLKK
                     SEIKTKRDQVDKVFVKARDEVEAVSAKDSELAAAQDKAQKEIEATQGDYRKVEDLTKK
                     LNELTDERKKVDAKLEELEANFNKIKELKAKIDGNIEKLSMQEDDLNKQLESTNVSLK
                     LEMDKATKEKAELEAVISPDALEAYKKARGIVGQTVIAKNEENTCSVCRANFTGANLS
                     KIESESPISTCPHCLRLLL"
BASE COUNT         2166 a         1627 c         1979 g         2133 t
ORIGIN      
        1 aaataatttg tcgatgcgaa agcatcagcg aaggtgagat tatggacgcg attaatcgtc
       61 cagtcggcgc gaagagcttg gacggcgtca agcgtcgtgt tcgcgcaggc atgggacgct
      121 gccaaggcgg cttctgcagc cctcgcgtaa tggaaatttt agcaagagaa ttaaaaaaag
      181 atattactga aattactaaa agaggcggaa agtcaaaagt acttttaggt aagaataaag
      241 aatgaaaata gaagaatttg acatagtaat aattggtgga gggcctgcgg gtttggcggc
      301 tgcggcttcg gcatgtgaag ccggctgcga aagcattgtc attttggagc gcgaccatga
      361 gcttggtggc atcttgaacc aatgcattca caacggcttt ggcttgcata cttttggcga
      421 agagctgacc ggccccgaat acgctgatcg cttcattaaa aaggtcaagg acttgggcat
      481 cgagcacagg ctgaacacct tcgttttaaa tattagcgaa gacaagcaaa tcacctgcat
      541 gaacaggacc gatggcctct acacaatcaa cgcaaaagct gttattttgg caatgggttg
      601 ccgcgagcga agccgaggcg ctcttaacat cccgggttac cgtccggctg gcatttttag
      661 tgcgggcacg gcgcagcgct tcgtcaatat ggagggctac atgcccggcc gtgaagttgt
      721 cattttgggc agcggcgaca tcggccttat tatggcgcgt cgcatgatgt tggaaggcgc
      781 gaaggtcaag gtcgttgctg agattatgcc ttttagcggg ggcctcaagc gtaacatcgt
      841 tcaatgcttg gatgattacg gcattccgct caagctcagt catacggttg ttgacatcga
      901 aggtcgcgag cgcgttgagg gcgtcacgat cgccgaagtt gatgagcaca tgaaaccaat
      961 tccaggcacc gaagagcact acagttgcga cactcttttg ctctcggtcg ggcttattcc
     1021 agaaaatgag cttagcctct ctgctggtgt cgagcttcac ccggtcacaa aaggtgcatt
     1081 cgtaaacgaa agtttggaga caagtgtccc tggaattttc gcttgcggaa atgtgcttca
     1141 cgttcatgac ctggttgatt ttgtctccga agaggcagca accgcgggtc gcaacgcggc
     1201 ttcgtttgta gagagcactc cgaagaaggg gcaaaacatc gaagttgtaa cgcaaggcgg
     1261 tgtgacctat tgcgtcccga gttttattga cccgacgaaa atcgaagaca agctcacctt
     1321 gcgtatgcgc gtgaacaatg tgtataaggg caagtactta agcctttatt ttgacgacga
     1381 gcgcatcaaa cacaagaagc gtcctgttat ggcgccgggc gagatggaga gcatcgagct
     1441 tacaaaagcg gatttcgacg cggcttcgtc gcttaaaaag ataacaataa aaatcgagga
     1501 taagtaatgg aaacgaagaa tttgacatgc ataaattgtc cgcttggttg ccaggttgag
     1561 gtcaagcttg acggcggtaa ggtcgtcgag gtcaccggta acacttgcaa aaagggcgaa
     1621 gaatatgcaa tcaaggaagt gaccaaccca acccgaatta ttacatcaag cgttattgtc
     1681 gaaggaggca ctggcacgaa atcgcaagtt tctgtcaaga ccgagcgcga cattcctaag
     1741 gataaaattt tcgatgttat ggctgcactc gctggcgtta gtgttaaagc ccctgtcaaa
     1801 attggcgatg tggttctcga aaatgtagct ggaacggggg ttaacattgt cgcaacaaga
     1861 aatatctaat taaatgcaaa aagccggagc aacagcaatg ctctcgctac aatcatcaaa
     1921 tacaagctgg ctgaaaaagc cttcgtcttt accaactcta atcacataag cattaattgc
     1981 attttgcgac atcggagatc ttagtcaata acttttgtca tctttgtttc ttgggtcatc
     2041 atcatcgcca ggcttataag ggtgagtggc ataaagtttg taaggcttgc cttcgacaat
     2101 tggattaatg ccagttatgt tcatctctgt cacgctaagt ggaaaaactt tttcgttaaa
     2161 tgttttaact tcgtcacttc cgccgccaac acaagtgtct ttgcgaacag tcttgatgga
     2221 cgatttcaaa tcggccggaa acatctcaaa taatttattg ttaagatagt tacgaagttc
     2281 tgaactctcc cattcgtttg agatcacgtt attctttgca aatggaacat tgctaataac
     2341 ttttgtaaat tcgaaggtta gagcagcggt tttaccattt tcgcaaacat cgtgattctc
     2401 cgcaataact cgaactttgt aatttccaat tccttcaaga ttgattcgtt tagttttggc
     2461 aagcgtttcg ttttcgtcat caaggtcttg aattatgaat gtgcctccaa ttggcttgta
     2521 agtttcaagc aggttgtcca agccgccttc ggcaactttt gcgattgtat ctcaagaatc
     2581 aaaagcaaag gacttgggtt cggcagtgct cttcccacac gaactaacgg cgaagaccac
     2641 cgcaaccgca atcgcgcaaa ttgttataac aaatgttgaa cgtccaattt tgtttgtcat
     2701 aattatctcc ttagtaatcg ttatggatta gtttcgaact aaattcgcca tttggcgtaa
     2761 gcgaaattaa acggccgcca agccagcctt cgtcatgata gataaagtcg cttgacttca
     2821 atccgtatgc taatttgatt ccttggtatt ctatgcacga atgatttacg tggtcatggc
     2881 cgcaaaaaac acccttggtt gattgaagtt ctttcatgac gtcaaagagt ccagtgtttt
     2941 ctcgagggca acaaatccct tcagtgtcgc gaccgtcgtc gagaaacttc gcttcgccac
     3001 gttgcactgc ttcgtaagca aataaatatt cttgcaatgg aatgtgaaaa aatgcaagcg
     3061 aaggcacaac cgcacctgaa ttcgcctcgg cagtttttgt gaccaattgc ttataccatt
     3121 caatttgatc cgggtagatg tagtcataat ccgaatagcc tttatcgctg gtagagtctc
     3181 tgtatctgtt gctgtcaaaa attatgagct gctctttgac cgtgttattt tggacaagat
     3241 ttatagcgaa gtttgcatcg ccatgaatat tatcgccaat gttgttttta aatttcaagt
     3301 aagaagttgg cgaagcagag cgctcgttga gaaaacttgt cagtttgtcc attgaaagtt
     3361 cagcgcgcgc atcgtgattg ccaaaagtaa gggttcaagg aattttaaag ctgtcgaaaa
     3421 aatcgcaata attttttcag tcatccagcg cagaatcgtt aacacaatcg ccggttatta
     3481 caataaagtc cggtcgagcg tttggaattt cagcgctcgt tattactttt gtaataaaat
     3541 ttttgtgagc ggttcaatcg gtgccaccaa gccagtgcgt gtcagtaagt tgcaaaactt
     3601 gaaaattgtc tttgtatggc acgtcaattg tgtagtcgtt gatgtttaca tttggcttgt
     3661 tattcttatt gcacgcaagc attccgcacg cgaggcacag gcacaaacaa cctaaaatac
     3721 taatttttgc aaacattttt ttcttcatag cacgctcctt ttccgcatat attttacgat
     3781 aaacgctcga aatcgctctt gtatattttt caaatttttg tcatcaaagt atttcggttt
     3841 tatataatac atgtatggct acttccaaaa caatttatct tgatgcggcg gcgagcgcga
     3901 gctcttcgga gtttgcgatc accgacaact ttgcaaatcc aaattcaatt cacgaagccg
     3961 gccgcgatgc cttcgctgtt atggaggccg cgcgcgagga cttcgcgaag ttgattgggg
     4021 cgaagcgtcc aagcgaaatt atttggacgt ccggcgcgac cgaggcgaac aacatcgcaa
     4081 ttttgggaat tccgcaagcc attacgcctg atccaaagcg tgggcagcgc aacaagatta
     4141 ttgtttttga gatcgagcac gaaagcgctt tgcttccggc taagcatctt gctcacgaag
     4201 gttatgatgt tcaaattttg ccgactgaca gaaatggttt cgcaaagctt atgaagtttg
     4261 ttgaaatttg cgatgaccgg accgcgcttg ttattatgca gcacgccaat actgaaattg
     4321 gaagcatcca aaacgttgcc gagttttgtg aaattgctca cgaaaatggc gcgctttttc
     4381 attgcgattg cgttggaagt tttggtcagg tgccaatcaa tgttgagaaa tatgggtttg
     4441 attcggcgag tttttctggt cacaagtttg gcgctccgaa gggaattggc gccctttact
     4501 tgaaggctaa caccccatgt aaacccatta tttttggtgg aagtcaagag cgtgagcttc
     4561 ggcctgggac tcaaagtgtt gcgttaactt cggcgtttgc aaaggcagct gaaatcacaa
     4621 agagtaatta cgatgccgtt ggaaaaaatt atgaagaatt cacgaatttg atcgcgtcgg
     4681 aaattgggaa gcttgatggc gttcggctga gcgttccttt caaggataat aaaatgggtt
     4741 atttgtgaaa catgatgagt ttggtttttg cgggccgtga tagcaagaat ttaattttgg
     4801 aatacggcaa acgaggcatt attgttgctg gtgggccggc ctgctcttcg gagtccgaag
     4861 agccaagcca cgtgcttcag gcaattggcg tgcccgaaaa tgaaatcaac ggcgccattc
     4921 gaatttcgtt taatccgcaa actactcgcg aagatattga ggcgttcatt aatgccacga
     4981 aggagattct aggtgaaaat tagagagctt gaaaaattga tgttggaaaa atttccgctt
     5041 gagaccgccg aagaatggga cacaacgggt gtgacttcgg gtgacgaaga caaggaaatc
     5101 actcgggttg cagttgcgct tgaccccagc atttccgcag ttaaagaggc aaaaaaacga
     5161 ggttgcaact tgcttgtcac gcatcatccg gcctacattg gagaaattgc gggccagcct
     5221 ttgcgcaaca taattttcca cgcgccaaat tacggagtgg cgcttatgaa ttttcacact
     5281 tgtttagatg ttagtgacga aggcgcggca actcttccaa acttgattgg acttaacgcg
     5341 aagcagacgc taattctaac ttgtggcaag cttggcttcg gaaagatttg cactccagca
     5401 aatgggaaaa gcacaacttt gagtgcactt gcaaaaaagt gcgagagcgt cttcggacgt
     5461 aagcctcgcg tgtggggaaa ggccagctca tcgcttaaaa aaattgtgac aacaaccggc
     5521 agtgttggtc gcttggatga cgaagttagc gtgcttcagg ccgccattga caaacgcgtt
     5581 gattgcataa tttgtggtga ggttaaatat catgatgcca tcgagatgct tgagcgtggc
     5641 atttgcatca ttgagcttgg gcacgatgtc agcgaacttc cgctttgcga tgttctgaaa
     5701 aattcacttg tggattgtgg catcgcgaaa atagatatta ttatgatcga ccaaaacaat
     5761 tattgaaagt aggtaaaaat gtttatgaaa aaaatcaagt acattagtgg aggcattttt
     5821 attgcaattt gcatttgcgt tttgtgtgtc ctgacctcgt gtgttagcag tcaggctcca
     5881 aaggtttcag ctgatgcgca aggcttgcat catcatgatg gctacacgct tgagcaaatg
     5941 acagttgtgt cgcgccatca aattcggtcg gcgctttcgg agcacgggtc tgaccttgca
     6001 aagatcaact ctcacgaatg gttgccttga tcttcgagcc caagctattt aaccgacctt
     6061 ggttgcgtgc aagaaacttt gatgggaatg tatttccgca gttttttgga atacgaaaaa
     6121 tttattccgg aaaattggtt tcctgccgaa gacgagacct acttttattg taattcgcaa
     6181 cagcgcacta tcgtgtcgac tcagtcattt tcgagtgcgt ttttgccagt agctaaccct
     6241 cgcattgttt ataagtgcga ttttgatacc gatgacccgg tcttttggcg agacattacg
     6301 aagagcagcg aagcttggcg caaccaggtt atgaaggaaa ttgaagagca agggggcgaa
     6361 gctggcctca acggcatcct tgagcaaaat gcgcaaaact tcaaaacact cgagaaggtt
     6421 ttggatttca agaattctga atatgcgaag gagcatggca ttacatcgct gcctttggat
     6481 gacatgaaaa taactttcaa ttatggcgaa ggtccttcga tgaagggaag tttgaagctg
     6541 ggtaactcgg cttcggatgc gctgaagttg caatattatg aatacccgga tgggcacgat
     6601 gcttcgtttg gtgttccgct ttcttgggat gaatggacac aaattggtgc gcttggcgat
     6661 gcctatcaaa agacgctttt tgggtctcac actgcagcaa tcgacaaggc caatccaatg
     6721 cttcaggaaa ttcaagcgga catgaaaaat cctaagcgaa agtttacctt ccttggcggg
     6781 catgatgaca acattatgtc gctaattgcg gcgcttcgaa tcaaggatta taaattggaa
     6841 agctcgctta attcgcgttc gccagttggt tcaaacttca ttatttcgaa gttcaaagat
     6901 gaggctggtc aggaattttg cacaatcagt ttgctgtatt gcacggcttc gcagcttcgg
     6961 agcttagatg ttttggattt ggaaaaccct ctaatcgaaa agcagttgga gtttgagggc
     7021 ctggagaaaa atgcggatgg catgtataag ttggcggatg tggaaaagcg catcgccgat
     7081 acaattaacg agtataattt gtggcagtag ttaggggttg ttttggctgg taaggttgaa
     7141 gatattgaga agttgcttcg gttgcaggag gtggataaca ccatcgaaga cggcgccgag
     7201 gcttttaata attcgccgat tgcgactgaa attggcaagg tgcgtttgaa gaagtccgag
     7261 attaagacga agcgtgacca ggttgataag gtttttgtga aggcgcgtga cgaagttgag
     7321 gccgtaagtg cgaaggattc ggagctggcg gctgcgcagg ataaggcgca aaaagagatc
     7381 gaggcaacgc agggcgacta ccgcaaggtt gaggatttaa cgaagaagct taacgagctt
     7441 actgatgagc gcaagaaggt tgatgcgaag ctcgaggaac ttgaggccaa cttcaataag
     7501 atcaaggaat tgaaggcaaa gattgatggc aacattgaaa agcttagcat gcaagaggat
     7561 gatttaaaca agcaacttga gtcaacgaac gtgtctctca agcttgaaat ggataaggcg
     7621 acgaaggaaa aagctgagct tgaggcagtg atttcgccgg atgctttgga ggcttataag
     7681 aaggctcgcg ggattgttgg gcagactgtt atcgccaaaa acgaagagaa cacttgttcg
     7741 gtttgtaggg cgaatttcac cggcgccaac ctctccaaga tcgaaagcga atccccaatt
     7801 tccacttgcc cccactgcct ccgcctcctc ctttaaaaag atgtaattct tgccaggcaa
     7861 taattacacc ttttggtgca tttcatgcca ggcaataatt acacc
//
LOCUS       DBNCDC010000054         7448 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_4935_length_7448_cov_8.524415, whole genome shotgun sequence.
ACCESSION   DBNCDC010000054 DBNCDC010000000
VERSION     DBNCDC010000054.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 7448)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7448)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7448
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4935_length_7448_cov_8.524415"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /locus_tag="AC04VQ_04820"
     CDS             complement(<1..1353)
                     /locus_tag="AC04VQ_04820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HIO9553478.1"
                     /translation="MQELIQKIKPLLRDIKHPSRYIGGEFGAPEIDAAGDQFNFCMVY
                     PDVYDIGQSNQAVRILCNKANEVEGIAAQRSFLPEPETIEKFKTNGIPMFSLETFSPL
                     SEFDAIGITVSHDLVATNICEVLDFAGIPIFAKDRNIPANIAPVPLIFGGGPMCCNPE
                     PYHAFFDVITIGEGEVATIEALKLVKKLKAEGKQRDEILVELSKLEGIYVPSLNDGKT
                     TVKRAVYKDFAKSDAFDNCIVPFAQIVHNRLNVEILRGCARGCRFCQAGIMYRPVRER
                     SAKNICDGVTIGLEQTGHDEVSLTSLSSTDHSQIEEILSTLNKNFDGSDKRVSIPSQR
                     LDAFGVDMARLVAGNKKGGLTFAIEAGSQRLRDVINKNVTEADIESATKFAFENGWNR
                     AKLYFMIGLPTETDEDLLAIAQTCEHVLEVARASAGEKRRHGINISASCAIFVPKAQT
                     P"
     gene            complement(1329..1805)
                     /locus_tag="AC04VQ_04825"
     CDS             complement(1329..1805)
                     /locus_tag="AC04VQ_04825"
                     /inference="COORDINATES: protein motif:HMM:NF016983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HIO9553479.1"
                     /translation="MDLSIKRKLTDWLYIYSEAVQISYAAHFPFLRSAVSIRVINACA
                     IENAGVGALKLLPGADMPIMTLNQGRMILELAAIYGYNITLDRLVEIVVLVLSAFGFR
                     ALSNYINNEVPIPDFIVDAVFGFGGTQLLGYAAREYFARGLAPEGLVEKCKNLFKK"
     gene            complement(1860..2264)
                     /locus_tag="AC04VQ_04830"
     CDS             complement(1860..2264)
                     /locus_tag="AC04VQ_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553480.1"
                     /translation="MALPVDPNKMKKVADEVLEEIESNISVNIYVDSTANKKMVDSIS
                     AHFNLASDAVTLNFIGLEQKKLHMDLEPDFAIVLAGQDKYSVLVYEALQVKDVPTLIV
                     SLDPHYIIDAANSNKIAVRKADVICPTYSTYT"
     gene            complement(2242..3414)
                     /gene="rodA"
                     /locus_tag="AC04VQ_04835"
                     /pseudo
     CDS             complement(2242..3414)
                     /gene="rodA"
                     /locus_tag="AC04VQ_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
     gene            complement(3414..5549)
                     /gene="mrdA"
                     /locus_tag="AC04VQ_04840"
                     /pseudo
     CDS             complement(3414..5549)
                     /gene="mrdA"
                     /locus_tag="AC04VQ_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
     gene            complement(5556..6098)
                     /gene="mreD"
                     /locus_tag="AC04VQ_04845"
     CDS             complement(5556..6098)
                     /gene="mreD"
                     /locus_tag="AC04VQ_04845"
                     /inference="COORDINATES: protein motif:HMM:NF016019.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIO9553481.1"
                     /translation="MARDSVNTKIYIGGLIVIALQLFIGPIISINAVVPNLLLAYTIV
                     CIVIRPDKMHLVFAFVMGLIYDLCFSGPVGAMAFVMVVFSFIVAKVLLRLPEINLGIS
                     LLTIGVSVFFVELFYGVFQVSIMISASFMDILAYRILPCTIYDVILAFVMFAIMARLI
                     APPVTANTIHSSVNEVSNSR"
     gene            complement(6098..6937)
                     /gene="mreC"
                     /locus_tag="AC04VQ_04850"
     CDS             complement(6098..6937)
                     /gene="mreC"
                     /locus_tag="AC04VQ_04850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIO9553482.1"
                     /translation="MNTLWDDIFAILKRPVVKLAILILLSLGLCGIYAAEHEGGPIHV
                     VQNACRTVCTPLSSAGIGVKAGMSVLADKIFDNFADGETLSELKAENARLKTQIIANE
                     KYKQEAERLQALLNLKDQYQAQGIGAHVIATSGNAWNQTITIDVGSIDGVNAGQTVLG
                     TNGVLGQVDSVNLNSASVRLLTDPNSGVAVKILTNGADCILKGSLDGVLYLEGLDVEE
                     AANVGDIIVTSGLGGSYVEGLVVGSISQIISGDSGVNRKIVVNPIERYSTITEVFVIQ
                     ETN"
     gene            complement(6946..>7448)
                     /locus_tag="AC04VQ_04855"
     CDS             complement(6946..>7448)
                     /locus_tag="AC04VQ_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013979630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIO9553483.1"
                     /translation="TSSSLRLAGNKMDEAIAKYLRELLGIKIGERTAEIIKIKIGSIL
                     PFEDGHERDMVISGQDIVTQQPREVTIQSEDVREALIEPINEMVRNIKDTFKSTNPDL
                     ASDIIQNGILLTGGGGLLSGLDKYLTQELEIPVWVSDTALTNVVFGCLKVLETPAALQ
                     QTMSHK"
BASE COUNT         2335 a         1802 c         1369 g         1942 t
ORIGIN      
        1 cggagtttgt gccttcggaa caaaaatcgc acaagaagca gaaatattta tgccgtgtcg
       61 tctcttctca cccgccgaag ctcgagccac ttccaacaca tgttcgcaag tttgcgcaat
      121 ggcaagcaaa tcttcgtcag tctcggttgg aagtccaatc ataaagtaaa gcttggctct
      181 gttccatcca ttttcaaagg caaatttagt cgcactttca atgtcagcct ccgttacatt
      241 tttattaata acatcacgaa gccgctggct gccagcctca attgcaaagg tcaagccacc
      301 ttttttgttt cccgcaacaa gacgagccat atccacacca aaagcatcca agcgctgtga
      361 aggaattgaa acacgcttat cgcttccatc aaaattctta tttaaggtag ataaaatctc
      421 ttcgatttga gaatgatcgg tggaagatag cgaagtcaag gaaacttcgt cgtgtccagt
      481 ttgctcgagc ccgatagtaa ctccatcgca aatatttttt gcacttcgct cgcgcactgg
      541 cctatacata attccagctt ggcaaaacct gcacccacga gcacaacctc gcaaaatctc
      601 aacatttagg cggttatgta caatttgcgc gaacggaaca atgcaattat caaatgcgtc
      661 gcttttagca aaatccttat aaactgcccg cttaacggtt gtttttccat catttaacga
      721 aggcacataa atgccttcca actttgaaag ctcaactaaa atttcatcgc gttgtttgcc
      781 ttcggctttt aattttttga caagtttaag agcttcgatt gtggcaactt ccccttcgcc
      841 aattgtaatc acatcaaaaa acgcatgata aggctccggg ttacaacaca tcggcccgcc
      901 tccaaaaatt agagggacgg gggcgatgtt agctgggatg ttgcggtcct tcgcaaaaat
      961 tggaatgcca gcgaagtcca aaacctcaca aatattagtt gcaactaaat catgactaac
     1021 ggtaattcca atagcatcaa attcagaaag agggctaaaa gtctcaagcg agaacatagg
     1081 aatgccgttg gttttaaact tctcaatagt ctcaggctct ggtaaaaacg aacgttgagc
     1141 agcaatgcct tcgacttcat ttgccttatt gcacaaaatt cgaacagctt gattgctttg
     1201 cccaatgtca taaacatccg ggtaaaccat gcaaaaatta aactggtcgc ccgccgcatc
     1261 aatttcgggc gcgccaaact cccctccaat atagcgtgaa ggatgtttaa tatcacgaag
     1321 taagggtttt attttttgaa taagttcttg catttttcaa caagaccctc aggagcaaga
     1381 ccacgagcga agtactcgcg cgcagcgtaa cccaaaagct gagttccgcc aaatccaaaa
     1441 acagcgtcaa cgataaagtc cggaattggg acttcgttgt ttatataatt agaaagagcg
     1501 cgaaaaccaa aggccgaaag cacaagaacc acaatttcaa caagcctatc aagcgtgatg
     1561 ttataaccat aaattgcagc gagctctaaa atcattcgtc cctgattaag cgtcataatt
     1621 ggcatatctg caccaggcaa aagcttgagt gcgccaactc ccgcattctc aatcgcacaa
     1681 gcattaatca cacgaataga aactgcagaa cgcaaaaatg gaaaatgcgc agcgtaagaa
     1741 atttgaacgg cttcagaata aatatagagc caatcagtaa gtttacgctt tatgcttaaa
     1801 tccatcgaag aattgtaatc cgaaaaatca atagcatcct tgccttcgct attcttacct
     1861 caggtgtaag tactataagt tggacaaatt acatcggcct ttcgaacagc aatcttatta
     1921 gaatttgcgg catcaataat atagtgagga tccaggctaa caatcaaagt tggaacatct
     1981 tttacctgaa gcgcttcgta aacaagaacc gaatatttat cttgaccagc aagaacaatt
     2041 gcgaagtccg gctcaagatc catgtgcaac tttttttgct caaggccaat gaagtttagc
     2101 gtaacagcat cgcttgccag gttaaagtgc gcgcttatcg aatcaaccat ttttttatta
     2161 gcggttgaat ccacataaat gttaaccgag atgttagact caatctcttc tagcacctca
     2221 tcggcaactt ttttcatttt attaggatcg actggaagcg ccataattaa atttcctcct
     2281 cttcgcaaca atcccgttaa ctgcgaccga cccaataaag ccaagcatta cgcagtttac
     2341 aatcataaac gatgacccat aactaataaa cggaagcgga attccggtga tgggcatcaa
     2401 tccgcaacac atgccaacgt tttcaaaaat ctggaatacc cacattccaa cgcagcatac
     2461 taccagtagt ttaccaaata aatcatcggt tgctttcgcg attttaagcg acacaacaat
     2521 caaggccgca tatagaccga gcaaaattac aactccaata aagccaagtt cttcggccaa
     2581 aacgcaaaag ataaagtccg ttggagcttc aggcaaaaat ttaagcgctg cttgtgtgcc
     2641 gtttagcagc ccctttccaa aaaatccgcc agagccaatt gcaatttgag cctgacgaag
     2701 attataaccg ctgtcagacg tgtcattttc cggatcaata aacacaaaaa gacgctgcct
     2761 ttgataattt ttcaagaagt ggtattcaac ttcgccgttt tcgttgattg ttttaaacgc
     2821 ctcgtccaag acaagcaaaa agacgaagag aagcacaaag gccccaagcg ttcacaacaa
     2881 atatttaaga cgcgcgccac ccgcaacaag agcaaccgca gcaataaaca tataaacaag
     2941 accagttcca aggtcgggtt gagtcatgat gcacaaaaat gggataagca taataccaag
     3001 acttttcatg tattcgcgag aatcatccaa tcggccaccg tatttcgcaa ccacagcagc
     3061 atccaacatg ataaccgtaa tctttgcaaa ctcacctggc tgtatttgaa taccaagctt
     3121 aatccacgaa gccgcgccct taaccgtaac tccaagacca ggaatatgag ggcttaaaat
     3181 cagcacgaca ttaataatga taagcggcac aattgcttcg ctgaaatatt tgtagtcgaa
     3241 gcgccaaaaa atcagcatca aaattattcc aataattaca ccaataagct ggcgcgtgta
     3301 attgtagtct tcgttatttt gaactgcaga aaatacaatg atcaagccaa acaccagcat
     3361 agcaagtgta ataatgaaaa gcaatgttac gtatacgttt ttaagcttat ccattaatca
     3421 gtcctttgac tttgtccagc ggaaattttt accgaacgcc cggtcgatcc tggaacgtat
     3481 ccaatttcct ctttaagttt tccttctaac gaatccaagg ccgcacgcaa aacttttgtc
     3541 gcaaccggag tagcagcatc ggcaccgccg ccaccttctt cgataacgca agtgcaaaca
     3601 tattttggct tatcataagg cgcataacat gcagtccaag cgaagtccgc cttgccggca
     3661 acttcggcag tacctgtttt gatggcgcaa tctaaattgt tgcttttgac cgtaggagca
     3721 actccagtgt tttcttgccc aacaccatga agtgcatctt gaattgccgc taagttttct
     3781 gcgtgaacat caggagtgct aatttcttcc ggctcaaatt ttgcaacaac atttccaacg
     3841 ccatttctaa cttccttaag aagatgcggc ttcataattt ttccagttgc aacaccagca
     3901 taaccaagag caagttgaag gggtgttacc aaaacataac cctggccaat tgccatattt
     3961 gcgttgtcac cacctaccca agtagcctcg gtcggaatgt ccttaaaata ttctttcttc
     4021 cattcaggag ttggaacacg accaagctcc tcgccttcca aatcaatata tgtcgattgg
     4081 ccaaaattaa acttcttgat aaaatcttgc atagcggtct cgcccaaagt atctttgtgg
     4141 tcgaagaaat atttcccaat ttcataaaaa acaacatcgc aagattgaac aatacctgtg
     4201 cgaaatccaa tgccgccatg gcctgaatga agccaacact tttgttgaag tccagtgttt
     4261 catccatccc aaacaccttt gcaatctcaa gattgtccgc caccagcagc tccgcattca
     4321 agacccgcaa ggccggtgaa tgctttaaag gtcgaagccg caggataggt tcccgcaatt
     4381 gcacgattta gaagcggata gtgagagctt tcggtgttat ataaattcca cgtgtcttgc
     4441 gaaattccat ttgtaaaaat tccaggcttg taggttggac aatttgccat tgccaaaatt
     4501 gagccatctt cgacatccat aacaacaacg ctacctgcac ttcccttgcc agttcctaaa
     4561 attccgtctg gcgcaattgt ttgttgtagc gttgtgtcag ccaaatattg aagcgttgca
     4621 ttaattgtca aataaacatc cgaacctttc gaaggcgcaa tctcgctttt gacttcaaca
     4681 atatttccat cagcatccga aacaacaatt cgctcaccat gatccccact aagcatgctg
     4741 tcataaaaac tttcaactcc gtttttacca acaatgtcgc cggattgaac atcgcgacct
     4801 tcttcaattt caagaagttc ttggggtgac acctcggctg tataacctaa aacatgagca
     4861 cccagcgcac cataaggata ttcacgaact gtcctacttt caatggtaac accgtcaaaa
     4921 gcaggtccat gctccgaaat ataagccgct tgtttttttg taacatcact tgcaacaact
     4981 cttcggcttt gcgcacctga gctctgatcc aaaattctat ttctaacaac gttatatggc
     5041 aggcccaaaa caacagaaag tcgcgaaaca acatcgtggt ttgccccaac atcgccttcg
     5101 gccaaaacag tcaaacttga tctgttttta accaaaacgt gaccatttgt atcaaagatg
     5161 ttcccgcgcg gtgcaggagt ttttatcgaa gttgtggcgt ttgcgctgga tagcttcgta
     5221 aatttatcac cgctaagcag ttgcatcgaa aaaatcttcg cacccaaaac actaaaggcg
     5281 gcagcaacaa aaattccaac cgctctaaaa cgggttcgaa gatgcttgta agggtcaact
     5341 tcggcatcaa cctgacctcc accaacacga gcgtcaggat ttaagtggtc ttcgggcgca
     5401 acgccaacag tttcaagact ttcaacttta tcaatcttgg gagtttttgg cttatcgttt
     5461 tctcgatttt taacagcaat atataaaacg atggcagcaa ttgccagcac aacaacagca
     5521 gcaataccaa ctaaaattcc taaaagcatt taatttcacc tgctattgct aacttcgtta
     5581 acagaagaat gaattgtgtt tgcggttaca ggaggagcga tcaaccttgc cataatcgca
     5641 aacataacaa aagccaaaat tacatcgtaa attgtgcaag gtaaaattct gtaggccaaa
     5701 atgtccataa aagaagcact tatcataatt gaaacttgga aaacgccata aaaaagctca
     5761 acgaaaaaca ccgaaactcc aattgttaga agcgaaattc caagattaat ttcaggaaga
     5821 cgaagcaaaa ccttcgccac tataaagcta aacacaacca taacaaaagc catcgcgcca
     5881 accggcccac tgaaacacaa atcgtaaatt aagcccataa cgaaggcaaa taccaggtgc
     5941 atcttatccg gacgaataac aatgcaaaca attgtgtaag ccaaaagaag gtttggaaca
     6001 acagcattaa tgctaataat tggcccaatg aaaagttgaa gcgcaataac aatcaaaccg
     6061 ccaatgtaaa tttttgtatt tacagaatct ctggccatta gttagtctcc tgaattacaa
     6121 aaacttcggt gattgttgaa tatctctcga tagggttaac aacaattttt cggttaacgc
     6181 cactatcgcc tgaaattatt tgcgaaatgc tgccaacaac caggccttcg acataactgc
     6241 ctccaagacc cgaagtaacg atgatatctc caacgtttgc agcctcttct acatcaagac
     6301 cttcaagata aaggacgcca tcaagcgagc ctttcaaaat acaatccgcc ccgttggtta
     6361 aaatcttaac tgcaacaccg gaattagggt cagtcaaaag tctaacactt gcgctattta
     6421 ggttaacgct atcaacctga cccagaacgc cattggtccc caaaacggtt tgaccagcgt
     6481 taacgccatc aatactacca acatcaattg tgatggtttg attccaagca ttaccacttg
     6541 ttgcaataac gtgcgcacca atgccttgcg cctgatattg gtctttaaga ttaagcagcg
     6601 cctgaagacg ctcggcctct tgcttgtatt tttcatttgc aataatttga gttttgaggc
     6661 gagcattttc agccttaagc tcacttaggg tctcaccatc tgcgaagttg tcgaagatct
     6721 tgtcggccaa aacgctcatt ccagctttaa ctccaattcc cgcacttgat agcggagtgc
     6781 aaacagtcct gcaagcattt tgaactacat ggattggacc gccttcgtgt tctgcagcat
     6841 aaattccgca aagccccaaa gacagcaaaa ttaaaattgc aagcttaaca acaggacgct
     6901 ttaaaatagc aaatatgtca tcccataaag tattcaagac ctaaattatt tgtgtgacat
     6961 tgtctgctga agagcagcag gagtttcaag caccttcaaa catccaaaaa ctacatttgt
     7021 aagagcagtg tcgcttaccc aaactggaat ttcaagctct tgagtaagat atttatccaa
     7081 accgctaagc aaaccgccac caccagttag caaaatacca ttttgaatga tgtcagatgc
     7141 aaggtctgga tttgtgctct taaatgtgtc tttaatattt cgaaccattt cattaattgg
     7201 ctcaatcaaa gcttcgcgaa catcttcgga ctgaattgtg acttcacgag gttgctgtgt
     7261 gacaatatcc tgaccagaaa ttaccatgtc gcgctcgtgc ccatcttcga aaggcaaaat
     7321 tgaaccaatt ttaatcttaa tgatttcggc tgtgcgttcg ccaatcttaa ttccaaggag
     7381 ctcacgcaaa tattttgcaa tggcttcgtc catcttattc cctgcgaggc gaagcgaact
     7441 cgaagtta
//
LOCUS       DBNCDC010000055         7440 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_4948_length_7440_cov_6.024238, whole genome shotgun sequence.
ACCESSION   DBNCDC010000055 DBNCDC010000000
VERSION     DBNCDC010000055.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7440)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7440
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4948_length_7440_cov_6.024238"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /gene="recO"
                     /locus_tag="AC04VQ_04860"
     CDS             complement(<1..134)
                     /gene="recO"
                     /locus_tag="AC04VQ_04860"
                     /inference="COORDINATES: protein motif:HMM:NF023393.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIO9553484.1"
                     /translation="MAGKDLHTNAIVLHKTKLSETDLIITFLCEDGMQVRAVAKGARK
                     P"
     gene            complement(134..1042)
                     /gene="era"
                     /locus_tag="AC04VQ_04865"
                     /pseudo
     CDS             complement(134..1042)
                     /gene="era"
                     /locus_tag="AC04VQ_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
     gene            complement(1035..1382)
                     /gene="ybeY"
                     /locus_tag="AC04VQ_04870"
     CDS             complement(1035..1382)
                     /gene="ybeY"
                     /locus_tag="AC04VQ_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIO9553485.1"
                     /translation="MNSLAKFVLEAEGKDAVASVNFVDKAEIHKLNKQYRNIDAPTDV
                     LSFESDIEGDLGDIFICKEVAAENAKKFGTSLDSELNLLVVHGCLHLCGYDHMNDEEA
                     KIMESREEEILDE"
     gene            complement(1366..3090)
                     /locus_tag="AC04VQ_04875"
     CDS             complement(1366..3090)
                     /locus_tag="AC04VQ_04875"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HIO9553486.1"
                     /translation="MSVDIEKAYQHWLDNANDSLQEELKNMSEHDKLEAFSTSLAFGT
                     AGLRGFLRAGTNGMNIYTVGQATQGLANYLKANFKNPSVAIARDSRNMGWEFVKVCVC
                     VLAANGVTAYYSEDIESTPGLSFSVRKLHCSAGICITASHNPAEYNGYKAYGPDGCQI
                     TTKAANAISDEIAKTDVFEDVKFLNFRDAKDTEFAKLIGPEVREAFIDAACECSVVDD
                     EETARSQAANLNVTYTALHGTGYPEVMEALERNGFTNLHPVEEQAEPNGNFPTASYPN
                     PETDEALALGLEQAKANNSDILIATDPDADRVGVEVKHGSDYIHLNGNEIGILLFNYI
                     CEARAKRGDALSNLVMCTTIVSSDIVDEIAKHYGVEERRVLTGFKYIGEQILHLEEDD
                     EKYRFMFGFEESCGYLAGTHVRDKDGISACLLIAQMAAMYKAQGKDLVDVLEDIYKQF
                     GYYKNGVISVEYPGVEGQDKMRDVLANLRSNPIKEYCGLKVVASKDYKEDTKMLVLNG
                     DEKSPDEKLPKSDVLEYSLEGGSKIIVRPSGTEPKIKVYCFSNADTESKSQKLLDKFK
                     ESANELLS"
     gene            complement(3099..3785)
                     /gene="secF"
                     /locus_tag="AC04VQ_04880"
     CDS             complement(3099..3785)
                     /gene="secF"
                     /locus_tag="AC04VQ_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014416.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIO9553487.1"
                     /translation="MAVVALIHDLILVIGVYAIFGREISPNTIAALLTILGYSLYDTV
                     VVFHRINDNMKQGTERCSFITMANHSINQVFVRSLNTTMTSLIPVVAMLFFGGETLKD
                     FAFAMSIGLVCGAYSSIAIACPLYSWWKTREPKNYRLMRKFGPDPDLFCFVAPETAHE
                     LGLSEFVAPTTNKAESSDAPKTTAVSAAEKAAQKAKVERELDFNAPKTKTPKPKKQKK
                     IKRPVKKNKK"
     gene            complement(3813..5765)
                     /gene="secD"
                     /locus_tag="AC04VQ_04885"
     CDS             complement(3813..5765)
                     /gene="secD"
                     /locus_tag="AC04VQ_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009608893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIO9553488.1"
                     /translation="MAKIKDARKKFGKLKKSTRRNVWLLILCTVLIIGSIFAFTPPQE
                     KINQGLDIQGGLSVVLSAKSTGEDATVSQEDMEKSRSIIEQRVNALGASEAVVQLQGN
                     DQILVQIPGMSDTQQALETIGKTGKLVFARLDSFTDEAVKEKIENNNYGENSTVSDDF
                     GVSFSTGDTQKLKVKEGTYTPLITGENLQNVTVGKASETSSDYAVNLTLDGEGTSAFA
                     AATKELAADKGKIVIILDDEVQSAPAVQSEITGGQVQITGGYDLDGANQMKTVLDSGS
                     LPVNFEYLQSQVVGPTLGQDALNSGVLVALIGLLIVMLYLLFFYKGLGTITAAAMVVF
                     ATMYLGILGLLSQLGFFSLSLSGIAGIVLTIGMAADSSVLTLERFREEIRMGRTVKQA
                     SKTGVHHAIMTSIDADSVTLVSALALFFLATATVKGFGLTLALGIVCDIVMMLIFKAP
                     LIRLLAPRVMARHPKFWDIDDSLKYSGGVEKLLNGEEKEVSHIVNAAKKIKGRFIKKD
                     INFLKWRKVFLCVAAGAMVVCFAFFGFKGLNYSIEFVGGTSVTFHGTGDTQIEDIRAA
                     FDEAGEPDAVIQTTVQDGEPGFLVRTTTTSSEDATASAKEVADKFGWTTENFEVTTIG
                     PDWGTSVIQSSLIAFLVSIILIIIYI"
     gene            complement(6109..7293)
                     /locus_tag="AC04VQ_04890"
     CDS             complement(6109..7293)
                     /locus_tag="AC04VQ_04890"
                     /inference="COORDINATES: protein motif:HMM:NF037085.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HIO9553489.1"
                     /translation="MIVITPKTDASASFVGAPYSKMYGDKDPDFEINFQGFLPVDTPK
                     LYEDYTIFRESGEDVNADGTTYRVIFNWLNPNYTILSMETTLTINKTNKYRLVCDNVS
                     KVYYEPDPASFTAHMEGLKFDDELTSTDYEIEKTPTNANPTTYKLKPKFSSSVSDRIK
                     NNYVIDDGSCFVEGTYKVYENPASFEFQFMVNTYDSYYDGQDHEFSINVKNPTSGAKV
                     QYKKEGEADWHDEPCSFKDVGTYNIYYRLTHDSCVPLEDSATLNIQKAPATVVPNACS
                     KNEGEADPALTAKVYGLVDGESLQPGEDYILQRQPGETPGMYDIYCTPADSEVANNYD
                     LTGADSKFFIASAGGNTKNVDGSGASGAKTGDANAAIFIAIAIIALASAGYIFSRKLR
                     KI"
BASE COUNT         2156 a         1770 c         1338 g         2176 t
ORIGIN      
        1 ggttttcgcg cgcctttcgc aacagctctc acctgcattc catcttcgca taaaaatgta
       61 atgatcaaat cggtctcaga aagcttcgtc ttatgaagaa caattgcgtt tgtatgtaaa
      121 tctttaccag ccattattca gccgtatatc caaattttct aacttgatta taatcgcggc
      181 gccaatcttt tttcagctta acttccaaat caagaaaaac cttcgtcccc aaaagttttt
      241 cgaggtcctc gcgcgcctcg gtgctaattt tcttaatagt ttgaccgtct tttccaatta
      301 agattccctt ttgactttca tgctctacaa ctgcatgagc ataaatgttg ttgattttct
      361 ttttctcatc gaagctaagc tccacaactt caagacccac ggaatgtgga acttcgtcaa
      421 aaacatttcg aataattttc tcacgaatat attcagcaat cataacctca attggttgat
      481 cagtttttgt gtcttgcgga aaccacttcg gaccttccgg caaaaaatgc tcaacctctt
      541 cgacgaaggc atcgatgttg tagtctttca ccgcactcaa acaaattagc gcatctcaat
      601 tttcaagctc agcagccttt gcaacttgag ccatcttcgt gtctttatca accttatcag
      661 tctttgtcaa aatgcaaaac tttttgcact tcaatctatt aaccttttca gcaacccacg
      721 catcgccttt gccaattttc tcgctcgcat caatgagcat gcacacaata tcgcaatcgg
      781 cgatagcttg aaaaacacct tcgtttagct cttgtcccaa aacatccacc ggcttatgaa
      841 ggcccggcgt gtcaacaaat atagcttgca tgctgggcgt atttaggatg ccacgaatcc
      901 tgtgtcgcgt ggtttgaaca acatttgaag tgatagcaac cttcgtgcca attaccgcgt
      961 taagaagagt cgacttcccc gcattcgggc gcccgaccaa agtgacaaaa ccactcttaa
     1021 atttattttg cgtctcactc atctaaaatt tcttcctcgc gcgattccat aattttcgct
     1081 tcttcatcat tcatatgatc atagccacac aaatgaagac agccatgaac aaccaaaaga
     1141 tttaactcac tgtcaagcga agtgccaaac ttcttcgcat tttcagcggc aacctcttta
     1201 caaataaaaa tatcgccaag gtcaccttcg atatcagact caaaagacaa aacatcggtc
     1261 ggcgcatcga tatttctgta ttgcttattt agtttatgaa tttcagcctt gtcaacaaag
     1321 ttcaccgaag ccacagcatc cttgccttcg gcttctaaaa caaacttagc taaggagttc
     1381 atttgctgat tccttgaatt tgtcgaggag cttttgcgac ttactttctg tatctgcatt
     1441 gctaaagcaa tagactttaa tttttggctc ggtgcctgaa ggacgaacaa taatcttcga
     1501 accgccttct aaagaatatt ccaaaacatc agactttggc aatttttcat ccggcgattt
     1561 ttcatcgcca tttaaaacca acattttagt gtcttccttg tagtccttag aagccacaac
     1621 cttgagccca caatattcct tgattggatt agagcgaagg tttgccaaga cgtcacgcat
     1681 cttatcttgg ccttctacac caggatattc aacactaata acgccatttt tgtaatatcc
     1741 gaattgctta taaatatctt caagaacatc gaccaagtct tttccttgag ccttgtacat
     1801 tgcagccatt tgagcaatca acaagcaagc actaattcca tccttgtcgc gaacatgagt
     1861 accggccaaa tatccgcaac tctcttcgaa gccaaacata aagcgatact tttcatcatc
     1921 ttcttccaag tgcaaaattt gctcaccaat gtatttaaag cctgtcaaaa cacgacgttc
     1981 ttcaacgcca taatgttttg caatttcatc aacaatatcc gaagacacaa tcgtggtgca
     2041 catcaccaaa ttgcttagcg catctcccct ctttgccctt gcttcgcaaa tgtagttgaa
     2101 cagcaaaatg ccaatttcat ttccgttgag gtgaatatag tcgcttccgt gcttaacttc
     2161 aacaccaact ctgtcagcat cagggtctgt tgcaattaaa atatcagaat tattagcctt
     2221 cgcttgctca agcccaagag ccaaagcttc gtcagtctct ggattaggat aacttgcagt
     2281 tgggaaattt ccattaggct cagcctgctc ttccactgga tgaagatttg taaaaccgtt
     2341 gcgctcgaga gcttccataa cttccggata gcctgttccg tgaagagcgg tgtaggttac
     2401 atttaagttt gcagcttgac ttctggcagt ttcttcgtca tcaacaacgc tgcactcaca
     2461 agcagcatca ataaaagctt cgcgaacctc tgggccaatc agctttgcaa attctgtatc
     2521 tttggcatca cggaagttca aaaatttcac atcttcaaaa acatcagtct ttgcaatctc
     2581 atcagaaatt gcattggcag cctttgttgt aatttggcaa ccatcagggc cataagcttt
     2641 atagccgttg tattctgcag gattatggct ggcagtaata caaattccag cactgcaatg
     2701 aagcttgcga actgaaaaac tcaaacccgg agtgctttcg atgtcttcgc tgtaatatgc
     2761 agtaacgcca ttcgcagcca aaacacaaac gcaaacctta acaaattccc atcccatatt
     2821 gcgcgaatca cgagcaattg caacacttgg gtttttgaaa ttagccttca aataattagc
     2881 gaggccttgg gtcgcctggc caactgtgta gatgttcata ccatttgttc cagcacgcaa
     2941 aaatccacga agaccagcag tcccaaatgc aagagacgtg ctaaaagctt cgagcttatc
     3001 gtgctcggac atattcttta attcttcttg aaggctatca ttagcattat caagccaatg
     3061 ctggtaggct ttctcaatat caacagacat tttatcctct atttcttatt cttttttaca
     3121 ggacgcttaa ttttcttttg cttcttaggc tttggcgtct ttgtttttgg agcattaaaa
     3181 tcaagttcac gctcaacctt cgccttttga gcagcttttt ccgcagcgct tacagcagtt
     3241 gtttttggag catcactgct ttcagccttg ttagttgtag gagcaacaaa ttctgaaagt
     3301 ccaagctcat gagcagtttc aggagcaacg aagcagaaca aatctgggtc tggaccgaac
     3361 ttgcgcatga gtcgataatt ctttggctcg cgagtcttcc accaagaata aagtggacag
     3421 gcaatcgcaa tcgaagaata agcaccgcaa acaagaccaa tgctcattgc aaacgcaaag
     3481 tcttttaagg tctcgccacc aaagaacaac atcgcaacaa ctggaataag cgatgtcatc
     3541 gtagtgttga gcgaacgaac aaatacctgg ttgattgagt gatttgccat agtaatgaag
     3601 ctgcatcgct cggtaccctg tttcatgttg tcgttaattc tgtgaaatac aacaaccgta
     3661 tcatagagcg aataacccaa aatggtgagc aatgcggcaa ttgtgtttgg cgaaatctcg
     3721 cgcccgaaaa tcgcataaac accaataacc aaaatcaaat cgtggataag ggcaaccaca
     3781 gccataacac ccatcttaga ttcaaatcta attcaaatat aaataataat caaaataatc
     3841 gaaaccaaaa atgcgatcaa cgaagactga ataacgcttg tcccccagtc aggaccgatt
     3901 gttgtaactt cgaagttctc ggtcgtccat ccaaatttat ctgcaacttc cttagcagat
     3961 gcggtcgcat cttcggagct tgtggttgta gtacgaacca aaaagccagg ttcgccatct
     4021 tgaactgttg tctgaattac agcgtcgggt tcgccggctt cgtcaaacgc agcacggata
     4081 tcttcaattt gagtatcgcc tgttccgtgg aaagtaaccg aagtaccacc aacaaactca
     4141 attgaatagt ttaggccttt aaaaccgaag aaggcaaagc agacaaccat cgctccagca
     4201 gcaacgcaca agaaaacttt gcgccacttc aaaaagttga tgtccttctt aataaaacgg
     4261 cctttaatct ttttggcagc attaacgatg tgcgaaactt ccttctcctc tccgtttaga
     4321 agcttttcaa caccgccaga atatttcaag gaatcgtcaa tgtcccaaaa cttgggatga
     4381 cgcgccataa cacgaggtgc aagaagacga atcagcggag ccttaaaaat gagcatcata
     4441 acaatgtcgc aaacaatacc taaagccaag gtcaaaccga agcccttaac tgtcgcggtt
     4501 gccaagaaaa ataacgcaag cgcggaaaca agcgttaccg aatcagcatc aatagaagtc
     4561 ataattgcgt gatgaacacc agttttgctt gcctgcttaa cagtacgccc catacgaatt
     4621 tcttcgcgga aacgctctaa cgtcagaacc gaagagtccg ccgccatacc aatggtcagt
     4681 acgatacccg caatgccaga aagagacaga ctgaaaaaac caagctgaga taacagacct
     4741 aaaattccaa gatacatagt tgcgaagaca accattgccg ctgctgtaat tgtgcccaaa
     4801 cccttgtaga aaaagagcag gtaaagcata acaataagaa gaccaatcaa agcaaccaaa
     4861 acaccagaat tcaaagcgtc ctggccaagt gttggtccta caacttgact ttgcaaatat
     4921 tcgaagttta ctggaagcga accagaatca agaactgtct tcatttggtt tgcgccatcc
     4981 aggtcataac ctccagtaat ctgcacctga ccaccagtga tctcactttg aacagccggt
     5041 gcgctttgaa cttcgtcatc caaaatgata acaatcttac ccttatcggc agcaagttct
     5101 tttgtggcag cagcaaacgc cgaagttcct tcgccatcaa gtgttaggtt aactgcataa
     5161 tccgaagacg tctcgcttgc cttaccaact gtaacgtttt gtaaattttc accagtaata
     5221 agaggtgtat atgttccctc tttaacttta agtttttgag tgtcgccagt agaaaaactt
     5281 acgccgaaat cgtccgaaac cgttgagttt tcgccgtaat tgttgttttc aatcttttcc
     5341 ttaacagcct catcagtaaa agaatccaag cgagcaaaaa ccaacttacc agttttacca
     5401 attgtttcaa gtgcttgctg agtatcagac ataccaggta tttggaccaa aatttgatca
     5461 ttgccttgaa gctggacaac tgcttcgcta gcacccaagg cattaacacg ttgctcgata
     5521 atgcttcgcg atttttccat gtcttcctgc gaaacggttg catcttcgcc agtgctcttc
     5581 gcgctcaaaa ccaccgaaag accaccttga atgtccaggc cttggttaat tttttcttga
     5641 ggcggagtaa atgcgaaaat tgatcctatg atcaaaaccg tacataaaat aagcaaccaa
     5701 acgtttcttc tggtgctctt ttttaactta ccaaatttct ttcttgcatc cttaatttta
     5761 gccatttaaa tcatcctttt tccacgtcta aaatctctat atacaacctg tatatttaac
     5821 cacaaaaacg ccttaataca accaattatt tattcgaaat ttaagtcatg ggcatttggt
     5881 ttcgtgggtc gctcaggaat atactttttg ctcaccgccc gctcgtcgag ctaacttcgc
     5941 aaataattgg tcaagttacg ttttttgaat tcctaatcca aaaaactact tgaccatatt
     6001 atttactcag tggatctctc ctcgctctct tgcgagttcg caaaaaatac attcctgcgc
     6061 tcctcctcag agacgacaac tcaaatcttc accgactatt taataaagtt agattttacg
     6121 aagttttcta gagaaaatat atcctgcaga agcgagggcg attatcgcga ttgcgataaa
     6181 tattgcagcg tttgcatcgc cggttttggc gcccgaagca ccactgccat cgacgttttt
     6241 ggtatttcca cctgccgaag caatgaagaa cttcgagtct gcacctgtaa gatcgtaatt
     6301 atttgcgact tcgctgtctg ctggtgtaca ataaatatca tacattcctg gtgtctcacc
     6361 tggctgacgt tgcaagatat aatcctcacc tggctgcaaa ctctcgccat caacaagacc
     6421 gtaaacctta gccgttaatg caggatcggc ctcgccttcg tttttactgc atgcattcgg
     6481 gacaacagtt gcaggtgctt tttgtatatt taatgttgca gaatcttcta gcgggacaca
     6541 tgaatcatgt gtgagtctat aatatatatt gtaagttccc acgtctttaa agctacatgg
     6601 ttcatcatgc caatcggctt caccctcttt tttatattga acttttgcac ccgaagttgg
     6661 gtttttaacg ttaattgaaa attcgtgatc ttgaccatca taatagctgt catatgtatt
     6721 taccataaat tggaattcaa aagatgctgg attttcataa actttatagg taccctcaac
     6781 aaaacaagag ccatcatcaa tgacataatt gtttttaatt ctgtcgctaa cacttgaaga
     6841 aaatttaggc tttaatttat atgtcgtggg gttagcgttg gttggagttt tttcaatttc
     6901 ataatcggtt gatgtaagct cgtcgtcaaa ctttaagcct tccatgtgtg cagtaaatga
     6961 ggccggatca ggctcgtaat aaaccttgct tacgttatcg caaactaacc tatatttgtt
     7021 tgtcttgttg atggtcaacg ttgtttccat tgataaaatg gtgtagtttg gattaagcca
     7081 attaaatata actctgtaag ttgttccatc agcgtttacg tcctctcctg attctctgaa
     7141 tatcgtgtaa tcctcataaa gcttaggtgt atcaactgga agaaagcctt ggaagttaat
     7201 ttcgaaatcg ggatccttgt caccatacat ctttgaatac ggtgctccaa caaaactagc
     7261 actggcatct gtttttggag taatgactat catggctgag ccttcaaccg atacataatt
     7321 ttcagcagat agacgataat aaacttcgta ctcgccaaca ttcttttcga taacaggagt
     7381 aggacctcat gaacttccat ctctactata ttctatcgaa atttcttctg gatctattgg
//
LOCUS       DBNCDC010000056         7311 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_5139_length_7311_cov_6.895397, whole genome shotgun sequence.
ACCESSION   DBNCDC010000056 DBNCDC010000000
VERSION     DBNCDC010000056.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 7311)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7311)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7311
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5139_length_7311_cov_6.895397"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="AC04VQ_04895"
     CDS             complement(<1..315)
                     /locus_tag="AC04VQ_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553490.1"
                     /translation="MSEENKNQNQAPQQKKRAKIIAIVAASDFNEKNFMKSLQGNMFA
                     SIIAADAGYRHLDSRGIVPNIVIGDFDSLGYVPKEDKQASVQLKEDVDAGTPKNIRVM
                     QYP"
     gene            complement(322..693)
                     /locus_tag="AC04VQ_04900"
     CDS             complement(322..693)
                     /locus_tag="AC04VQ_04900"
                     /inference="COORDINATES: protein motif:HMM:NF026064.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553491.1"
                     /translation="MEDRMEFVGYANIVQAVALTGKKVQHIWESIDSAFANYCKKIST
                     NQLNNWLMKIKETGFTVTKGKQVLRLKYITQTGTKPPHFTAFVNHASIVNDNFERFFE
                     NRMRENFDLSGTPITVRYKTK"
     gene            complement(739..1626)
                     /gene="der"
                     /locus_tag="AC04VQ_04905"
                     /pseudo
     CDS             complement(739..1626)
                     /gene="der"
                     /locus_tag="AC04VQ_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014036.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
     gene            complement(1630..2520)
                     /locus_tag="AC04VQ_04910"
     CDS             complement(1630..2520)
                     /locus_tag="AC04VQ_04910"
                     /inference="COORDINATES: protein motif:HMM:NF019869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIO9553492.1"
                     /translation="MMNMEGVSPHEIVPNTEKDVDQWIMARLAVLVERVETAYKTYDF
                     GEVTKALYTFFWNEFCDWYIEFSKSRLNGTDEADKRVCQDNLLFVLEMSLRLLHPIMP
                     FVTEEIYQQLPMEHETKHLIMAAWPDASAFASFRDEASEVSIGLVCDIVGSARSIRAR
                     YGISPKQELEVVVKPKDNKLADLISSQVDLIKRLANTSELSVDAKAAKPNQSSVVVAN
                     GVEIYIVLEGLVDFDAERKRLTKELDALKKDEAMFSKKLSNPGFLQNAKPEVVEKDKS
                     KLAKIQENISLLETQIKELG"
     gene            complement(2548..4284)
                     /locus_tag="AC04VQ_04915"
                     /pseudo
     CDS             complement(2548..4284)
                     /locus_tag="AC04VQ_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012361.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
     gene            complement(4287..5768)
                     /gene="clpX"
                     /locus_tag="AC04VQ_04920"
     CDS             complement(4287..5768)
                     /gene="clpX"
                     /locus_tag="AC04VQ_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIO9553493.1"
                     /translation="MAKKNENKDNKVSELTDEMVCSFCGKKGSMVAGMIENGNGSYIC
                     DECVETCVDTFKRELNLSLGDKGLGNGTLTEAELRNIAMMDAMRNFPADYKNGYVAEE
                     VVYSTEGEKKKPFVLENLPTPSEMFDILSEYVIGQDEAKKALSVAVYNHYKRLQIAGI
                     AKGDEKANSKAEILAKEDTEISKSNILLLGPTGSGKTLLAQTLANILQVPFAIADATA
                     LTEAGYVGEDVESILLKLIIDADFDVDAAELGIVYIDEIDKIARKGENVSITRDVSGE
                     GVQQALLKIVEGCLANVPPKGGRKHPEQELIPIKTDNILFIIGGAFVGLTDIIAKRVG
                     DSAIGFNTKTTKKKNEQEAELLRQVLPQDLTKYGMIPEFVGRIPVVTSLSALEKDDLI
                     QILTEPKNSLLKQYTKMFAYENAELIFDRGALEAIADEAIKQKTGARGLRNICERVLL
                     DVMYQLPDVSGAKTVRVTKDDILGKSKPEIKKKNSTKKASTKK"
     gene            complement(5770..6378)
                     /locus_tag="AC04VQ_04925"
     CDS             complement(5770..6378)
                     /locus_tag="AC04VQ_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HIO9553494.1"
                     /translation="MKIESVTKNALVPYVIEQSDRGERSYDIYSRLLNDRIIFLGEQV
                     DDAVANTVIAQLLHLENSDPDKDISLYINSPGGSVTAGLAIMDTMNFIKPDVSTVCVG
                     LCASMAAVLLAAGAKGKRFALPNSTILIHQPMGQAQGQETEIAIVAEQIKKTREQLNK
                     ILADATGQDLKTIQKDTDRDNYMTAEEAKKYGIIDKIPTVHK"
     gene            complement(6451..>7311)
                     /locus_tag="AC04VQ_04930"
     CDS             complement(6451..>7311)
                     /locus_tag="AC04VQ_04930"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF017509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIO9553495.1"
                     /translation="KDSTVSLKIEAKDDAGKAIDTLTNDKLSYHLGSQFLPEDFEKKI
                     VGAKKGDKLDFTIDMPKPATVYTQALEGKTKKIKFKVEVNGIQVKKLPKIDDEFAKKN
                     MGFDTVKELREQIKAQVAQEKARMLPLLQENRSLDELAKRLKEEVPKQVCEKKEAQLL
                     QDFFGQLQQAGMTFDQYLASQNLDADKFKADLKKQAADVAKQDAALDAYAEKKGLLAT
                     DKEVEAEFKNGDPQNWEKLYEEWRSRGELHIVRQAITRMKAAKDLVDNAEVKVEGEKK
                     PAAKKTQTKK"
BASE COUNT         2015 a         1710 c         1298 g         2288 t
ORIGIN      
        1 ggggtattgc ataacgcgaa tattcttcgg agtgcctgca tcaacatctt ctttaagttg
       61 aacgcttgct tgcttgtctt ccttaggcac ataaccgaga ctgtcaaagt caccaattac
      121 aatatttgga acaatgcctc gcgaatcaag gtgtctataa cctgcatcgg ccgcaatgat
      181 gcttgcaaac atattacctt gcaaactttt catgaagttt ttctcattaa aatcggatgc
      241 ggcaacaatt gcaataatct ttgctctttt cttttgttga ggggcttgat tttgattttt
      301 gttttcttca ctcatgtcta cctactttgt cttataccta acagttattg gagttccgct
      361 caaatcgaaa ttctcgcgca tcctattttc gaagaagcgc tcaaaattat cattgacaat
      421 actagcatga tttacaaatg ccgtaaagtg tggaggcttc gtcccagttt gcgtaatata
      481 tttaagacga agaacttgct tacctttcgt aacggtaaaa cctgtttctt taattttcat
      541 caaccaatta ttaagttgat tcgtagaaat tttcttacaa taatttgcaa acgccgaatc
      601 tatagattcc caaatatgtt gaactttctt tccagtaagc gcaacagcct gaacaatgtt
      661 tgcataacct acgaattcca ttctgtcttc caagtgctgc cgaacttcgg ccttgcgctt
      721 cgcatctcca acaatatctc acttattcaa aacgatgacg agcgcgcatc ccctatcctt
      781 cgccattcct gcaactcgtt gatcttggtc tgtcaagcca agagttgaat ccacgacaag
      841 cacagcaaca tcggctttat caattgcgcg cattgctcga acaaaagaat aatattcaac
      901 atcttcgtca atcgcacttt tcttacgaag accagctgta tcgatgattg tatatttttg
      961 accggcacgc tcaacaatgg tgtcgatgct atctcgggta gttcctgcaa cgtcagaaac
     1021 aatcgatcgg ttctctcccg tcaaacaatt tgtgagagaa cttttgccag cattcggacg
     1081 tccaataatt gcaattttta tagaatcatc ttcttcttca ataacatttt gcggaaattt
     1141 tgtgatcatg tcgtccaaaa aatcgccagt gccatgacca tgaagagctg aaattgcata
     1201 aggatcacca ataccaagct tataaaattc ataaatgtca tttgtgttgt tgggctcatc
     1261 aattttattg acaaccaaca tgattggctt tttggagcgc ttcaaaattt gggcgacttc
     1321 ttcgtcttcg gactgaatgc cagttttgcc atcaacaata aaaataatta aatcgctctc
     1381 ttcggtgctt gcaagagctt gtgccttaat tgaattttga aattgatcac tgctcgaaac
     1441 ttcaattccg ccagtatcaa tcatgcggaa gcgaacgcca tttcaatcgg catcatgata
     1501 ggaacggtcg cgcgtaacgc ctcgcatctc gtgaacgata gcttcgtcac gaccagaaat
     1561 tcgattaacg aaggtggact tgccgacatt tggcctgcca actattgcaa ctaaaggcaa
     1621 actcatggct taccccaatt ctttaatttg agtttcgaga agagaaatgt tttcttgaat
     1681 cttggctagc ttgcttttat ctttttcgac aacttcaggt tttgcgtttt gcaaaaatcc
     1741 tggattgctt aactttttgg agaacatggc ttcgtctttc tttaaagcat caagttcttt
     1801 tgtaagtctc ttgcgttcag catcaaaatc aaccaaacct tctaaaacga tgtaaatttc
     1861 tactccgttt gcaacaacaa cgcttgattg atttggcttc gccgcttttg catcaacgct
     1921 tagctccgaa gtattagcaa gcctcttgat taagtcaact tgcgaagaaa ttaagtcagc
     1981 caatttatta tcttttggtt taacaacaac ttcgagttct tgcttaggtg aaattccgta
     2041 acgcgcacgt attgatcttg cgctaccaac aatatcacaa acaagtccaa ttgaaacttc
     2101 gcttgcttcg tctctaaagc ttgcgaaggc cgaagcgtcc ggccaagcgg ccatgatcaa
     2161 atgcttagtt tcatgttcca ttggaagttg ttggtaaatt tcttcagtaa caaaaggcat
     2221 aattggatgg agcagacgca gagacatttc aagaacaaac aacaaattgt cttggcaaac
     2281 gcgcttgtct gcttcgtcag ttccatttag gcggcttttg ctaaattcaa tataccaatc
     2341 gcaaaattca ttccagaaaa atgtataaag agcttttgtg acctcgccga aatcatatgt
     2401 cttatatgca gtttcaacgc gctcaaccag cacagcaaga cgagccataa tccattggtc
     2461 aacatccttt tcggtgtttg gcacaatttc gtgagggctg acaccttcca tattcatcat
     2521 aacgaagcgc gccgcattgc gaatcttatt tgcaaaattt cgagcatttt caatcttttg
     2581 cttgtcaaaa cgaattgctt gaccacccgt aacctgcgtt aaaagtccga agcgaagcgc
     2641 atctgcacca tattcgttga ttaaatcaat cgggtcgaca ccgttgcctc ggctctttga
     2701 cataggcttt ccgtctgcgc ccataactgt tgggtggatc aaaacatgtt caaacggaat
     2761 ttgcttcatg caatattcag acgccataac catgcgtgca acccaaagtc ccataatgtc
     2821 tctagcagtt gaaaggacct gagttggata gtatttatca agatcgccat ccggatcacc
     2881 atctgctcaa ccttgagttg caaaaggcca aagttgcgaa gagaaccaag tatcgagcac
     2941 gtcttcgtct tgtcggacct tggcaccaca ctttggacac ttatcaattt tatcaacgct
     3001 tgcatcttgc catccgcatt cgtcgcaata gaacattgga atttgatggc cccaccaaag
     3061 ctggcgagaa atgcatcaat cttttaagtt ttccaaccag tcaagataaa cctggcttca
     3121 gcgctcaggg aaaaattcga tatccccatc ttgaacaact ttaagtgcag gcttcttgag
     3181 ctcttcgaca tttacaaacc attgctcaga tagccaaggc tcaagcgttg tgtgacatct
     3241 ataacaatgc ataacgctgt gatcatgatc ttcgatgtgg tccaaaaggc caagtttgtc
     3301 aaattcttca acaatagctt cgcgagcctc aagcgcactc ataccagaaa acttgccata
     3361 accttcaaca acgtgagcag tctcatcgaa aatattgatt cgctcaaggt cgtgcctatt
     3421 acccatgccg taatcgttag ggtcgtgcgc aggagtaact ttaacagcac ctgtaccaaa
     3481 ttccggatca acataaaaat catcgaagat tggaatcttg cgatcaacaa ttggcaaaat
     3541 caaattcttg ccaatgagat ttttgtaacg ctcatcttta ggacttaccg caacacccgt
     3601 gtcacccagc atagtttctg gacgtgttgt tgcaatcaaa acgtgatcaa tgccatcaac
     3661 cggttcttct agcgggtatt ttaaatacca caagtggccg tgctcgtctt cgtattctgc
     3721 ttcgtcatcg gcaatcgcgg tcgtgcaatg agggcaccaa tttacaattc gcttgcctcg
     3781 ataaattaaa tcattgtgat atcaatcaac gaagaccttg cgaactgctc gcgaaaattc
     3841 atcgctcatc gtaaagtttt cgcggtcata atcaacagag caacccatct ctttaatttg
     3901 atttacgatg gttgttccat attcgcggcg ccactcttga cacgcttcga taaatttctc
     3961 gcgaccaatt tcgcgccgat taattccctg atcagcaagg tgtttatcaa cctttgtttg
     4021 cgttgcaata ccagcatgat cggtgcctaa aactcaacga gtttgatatc cttgcatacg
     4081 agcgcgacga acgatggcat cttgaattgc atcattgagc gcatgtccca tatgaagctt
     4141 tccagtaata tttggaggcg gaattacaac cgtgtaagat tcgcttgcat gcttaccaaa
     4201 acctgcggtg cgagcgaaat acccagcatc cttccactcg ttaaaaatct tttgatcatt
     4261 ttctttttga ttgttttgag ccatggttat ttcttcgtac ttgccttctt tgtagaattt
     4321 ttctttttaa tttctggctt ggacttaccc aaaatgtcat ccttcgtaac gcgaacagtc
     4381 ttggcccccg atacatctgg cagctgatac ataacatcca aaagcactct ctcacaaatg
     4441 ttacgaagcc cacgagctcc cgtcttttgt ttaatagcct catcggcaat agcttcaaga
     4501 gctcctctgt caaaaattaa ttcagcattt tcataagcaa acatttttgt gtattgctta
     4561 agaagtgaat ttttaggttc agtcaaaatt tgaattaggt catctttttc aagtgcagat
     4621 aacgaagtaa caactggaat ccttccaaca aattctggaa tcattccata tttagttaaa
     4681 tcctgtggca aaacttgacg taaaagttct gcttcttgtt cgtttttctt ttttgtagtt
     4741 tttgtattaa aaccaattgc actatctcca acgcgctttg caataatgtc agtgagtcct
     4801 acaaaagcac cgccaatgat gaacaaaata ttatctgtct tgattggaat gagctcctgt
     4861 tcagggtgtt tacgtccacc ttttggagga acattagcca agcaaccctc aacaatttta
     4921 aggagcgctt gctgcacgcc ttcgccagaa acatcacgag taatacttac gttttcacct
     4981 ttgcgtgcaa ttttatcgat ctcatcgatg tagacgatgc caagctcggc cgcatcgaca
     5041 tcaaaatcgg catcgatgat tagtttaagt aaaatgctct cgacgtcttc gccaacatat
     5101 cctgcttcgg ttaaagcagt tgcatcggca atggcaaatg ggacttgcaa aatgttcgca
     5161 agcgtttgag caagaagcgt cttgccggaa ccagttggtc ccaaaagcaa aatattgctc
     5221 ttcgaaattt cggtatcttc cttggccaaa atttcagctt tagagtttgc cttttcgtcg
     5281 ccctttgcaa taccagcaat ttgaagacgc ttataatgat tataaacggc cacactgaga
     5341 gccttttttg cttcatcttg accaataaca tattcagaca aaatatcaaa catctcactt
     5401 ggtgttggaa gattttccaa aacgaatggc tttttctttt cgccttcggt gctataaaca
     5461 acttcttcag caacataacc atttttatag tcggctggaa aattcctcat cgcatccatc
     5521 attgcaatgt tacgaagttc agcttccgtg agtgttccat ttccaagacc tttatcgccc
     5581 aggcttaaat ttagctcacg cttaaaagta tcaacgcagg tttcaacgca ttcgtcacaa
     5641 atgtaagaac cgttgccgtt ttcaatcata ccagcaacca tgctgccctt tttaccacaa
     5701 aaagagcaaa ccatttcgtc ggtaagttca gaaaccttgt tgtctttatt ttcgtttttc
     5761 ttcgccatat tatttatgaa ctgttgggat cttatcaatg atgccgtatt tcttagcttc
     5821 ttcggcagtc atgtaattat ccctgtctgt atccttttga attgtcttta aatcttgacc
     5881 agttgcatcg gccaaaatct tatttaattg ttcgcgagtc tttttaattt gctcagcaac
     5941 aattgcaatt tcagtctctt gaccttgagc ttgacccatt ggttgatgaa tcaaaattgt
     6001 tgaatttgga agagcgaatc tcttgccctt cgcgccagcc gctaacaaaa cagcagccat
     6061 gcttgcacaa agtccaacac aaactgttga aacatctggc ttaataaaat tcattgtatc
     6121 cataattgca agtccagcag tgaccgatcc accaggactg ttaatgtata acgaaatatc
     6181 tttatcagga tcagaatttt ccaaatgcaa aagctgagca attactgtgt ttgcaacagc
     6241 atcgtcaact tgctcgccca aaaagataat tctgtcattc aagagacgag aataaatatc
     6301 ataactacgc tcacctctgt cgctttgttc aataacataa ggtactaatg cgttttttgt
     6361 tacactctca attttcattg tccctcctat ttcttccttc ttcttaacaa caacaaaata
     6421 attattatta aaagtacggt attcattgtt ctacttcttt gtttgagtct tctttgcagc
     6481 aggctttttc tcgccttcga ccttaacttc tgcattatca actaaatctt tagcagcctt
     6541 catgcgagta attgcctgac gaacaatatg aagctcacca cggcttcgcc attcttcgta
     6601 tagcttctcc caattttgag gatcaccatt cttaaattca gcttcaactt ccttgtcagt
     6661 tgctaaaaga cctttcttct cagcataggc atccaaggcg gcatcttgtt ttgcaacatc
     6721 agcagcttgc ttctttaggt ctgccttaaa cttatcggca tccaaatttt gcgatgccaa
     6781 atattgatca aaggtcattc cagcctgttg caattgacca aaaaagtcct gcaaaagctg
     6841 agcttccttt ttctcacaaa cttgctttgg aacttcttcc ttcaaacgtt ttgcaagctc
     6901 atccaaactt ctattctctt gaagaagagg caacatcctg gccttttcct gggcaacttg
     6961 agccttaatt tgctcacgaa gttctttgac tgtatcaaaa cccatgttct tcttcgcaaa
     7021 ttcatcatcg atcttaggaa gtttcttaac ttgaatgcca ttaacctcaa ccttgaattt
     7081 aatcttttta gttttgcctt caagagcttg agtataaaca gtagcaggtt taggcatatc
     7141 aattgtgaag tcaagcttgt cgcccttctt agcaccaaca atcttcttct caaaatcttc
     7201 cggcaaaaat tgcgatccca aatgatagct gagcttgtca tttgtcaaag tatcgattgc
     7261 cttgccagca tcgtctttgg cttcaatctt tagagaaacg gtgctgtcct t
//
LOCUS       DBNCDC010000057         7054 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_5521_length_7054_cov_7.246750, whole genome shotgun sequence.
ACCESSION   DBNCDC010000057 DBNCDC010000000
VERSION     DBNCDC010000057.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7054)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5521_length_7054_cov_7.246750"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            62..601
                     /locus_tag="AC04VQ_04935"
     CDS             62..601
                     /locus_tag="AC04VQ_04935"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIO9553496.1"
                     /translation="MERLKKEIQNCSKCPLKDGRTNVVFGSGNQNADVMIIGEAPGKN
                     EDEQGLPFVGKAGQYLDELLALANLKREDVYIANVLKCRPPSNRNPRAEEIQACAPYL
                     REQTAKVNPKVIVTLGNFATRFILKNEEGITSLHGKPVQAGKFTVFPVFHPASTMYDP
                     KKKSALEKDFSKLKSIINK"
     gene            698..1159
                     /locus_tag="AC04VQ_04940"
     CDS             698..1159
                     /locus_tag="AC04VQ_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553497.1"
                     /translation="MKKIQHKANTRFGIGMILALLIVPALIIGICTVANTQTSYAKEE
                     AIQNGLNDFLATPLTEEEQALVASNADNATEIKLYGTATEQDVNGQIVAVKNLTIKFT
                     GVAPNGDSLDVSTTTNENGEYSITIPQFSKGVLFYVDEYASTENANYSSAR"
     gene            1376..1969
                     /locus_tag="AC04VQ_04945"
     CDS             1376..1969
                     /locus_tag="AC04VQ_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553498.1"
                     /translation="MNEYQSAALESNAIDSEYNTQIHVSGKTITYTFTINDIVYKETL
                     EAEETNSDYVFDGWYKKSLLGTRDMAANEVNKQQQQYKNYFGYCKVNDGDYLWDFAPD
                     SFGFEQTEESSADNAASASTNGAFCLYIARFVPKTNAHKVTFKVTDEAKFNHGYITGS
                     NGNTIDSADDALKEFSFNVNTTNMVAYGYDASIQKPA"
     gene            2171..3763
                     /locus_tag="AC04VQ_04950"
     CDS             2171..3763
                     /locus_tag="AC04VQ_04950"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIO9553499.1"
                     /translation="MPIDQDLTFVASFVKTRTLTGIVKDNAGNPLAHASVSFRDSNGY
                     RTEVQADAEGKFTMNVSALAGYLSAGKNGYTSETIEITEGTTTVDISLKTPENPVYLV
                     GNLFRFAAGGGGNAQRLNAEASNSYRSISDVTINYSINDYENYTYYVAGLQDEENADP
                     HYVSDFKMPIPEGSAGQLTFTHQGYQTMSFRLTSEMTQKTDPETLINVAKLSIPEADV
                     DPGKLDPSYEPPAIVLASGSDLNVFISVDDTNSGNKISFTNEQEGTPELLDYTAGMHY
                     RAGDKISVDFLSGTLTITTEGETEADTAKTIVTPVPNKEANYEFFGWVGYSSFGERIE
                     FMSEDTLQTDLNIYAQFKKAAQLTPLALSEGHESYTEEFYSNELKHVGEDKPIVVTKF
                     QPDPLRQYAGTKYSVVDNQIKFEYDESWENPDIGDVYKEQTTLTANAKDGWTFDYWLV
                     NGQRVDDTVTPIQISTDNLKLDVLAVYTSPDVPPTPVVDPTAGGTAQTGDGIPAAVLA
                     LCVLFLAGAAIAARRQGIKFTK"
     gene            3763..4092
                     /locus_tag="AC04VQ_04955"
     CDS             3763..4092
                     /locus_tag="AC04VQ_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553500.1"
                     /translation="MSYDSAFGQMMHHVFGGFDMSIFAAFGFVQNDIFTWLAHAFTAF
                     GQPEFCIMLFIMGLVMCAFKRTRKIGLLIICTIGLFFITNNLILKNLFFRLRPYNALQ
                     GNAEYFQ"
     gene            4111..4338
                     /locus_tag="AC04VQ_04960"
     CDS             4111..4338
                     /locus_tag="AC04VQ_04960"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042577 - lipid phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046839 - phospholipid dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIO9553501.1"
                     /translation="MAETETCFPSGHSCFAFSMATALFLWIRNDLKKSEGWMLFIPAL
                     FVACSRIYLMVHYPTDVIAGILEGILLGTIV"
     gene            4657..5472
                     /gene="murI"
                     /locus_tag="AC04VQ_04965"
     CDS             4657..5472
                     /gene="murI"
                     /locus_tag="AC04VQ_04965"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIO9553502.1"
                     /translation="MPNTTDIKNAPIGVIDSGIGGITVLRELLRTCPTEHFVYIGDDA
                     NSPYGTRTAEDIQQLSLALIKHPKLVHTKALVVACNTITAAALDDIAQNYEGPVFGVI
                     EPGIDEVLATTKNNHVGILATTATVESGVFERALKSANPDIEVTSVGAPKLHEFAENN
                     LDLVLGAPTQEIKECVVEHIAPFIDAGCDTILLACTHFPPYASLIRDIVGSDVNIIAP
                     SVNTAKALRTTLQDLNLTNQSSANQTHAHLNYEIISTAGNDEKFAKFSELLLK"
     gene            5472..6071
                     /gene="rdgB"
                     /locus_tag="AC04VQ_04970"
     CDS             5472..6071
                     /gene="rdgB"
                     /locus_tag="AC04VQ_04970"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIO9553503.1"
                     /translation="MKVLLATNNQNKAREIRNIVAGRDIEIYTLSDLNLASDPEETGQ
                     TFAENAAIKAEAALAEMKEKGFAGYAVVADDSGLCVDALDGGPGVYSARYAEVNGKPC
                     TYKDNNKKLLKALKKIPMAERTAHFETVAVLINEDGTRTEAVGQLFGTIGKHERGHNG
                     FGYDPIFIPEGTAITLAELSAKEKDEISHRGQAFRKLFQ"
     gene            6105..6180
                     /locus_tag="AC04VQ_04975"
     tRNA            6105..6180
                     /locus_tag="AC04VQ_04975"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6138..6140,aa:Arg,seq:tct)
     gene            6304..6360
                     /locus_tag="AC04VQ_04980"
                     /pseudo
     CDS             6304..6360
                     /locus_tag="AC04VQ_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013915.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6478..6639
                     /locus_tag="AC04VQ_04985"
     CDS             6478..6639
                     /locus_tag="AC04VQ_04985"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HIO9553504.1"
                     /translation="MSFHGGFIGGIIGGFFGAKIMGIKYLRLLDLAFIGVPIGLFFGR
                     CANFINGEL"
     gene            6694..6900
                     /locus_tag="AC04VQ_04990"
     CDS             6694..6900
                     /locus_tag="AC04VQ_04990"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HIO9553505.1"
                     /translation="MPRHPSQLYEAILEGLVLLAIMLILARKLPPYKEGTYFAVFCIF
                     YAVFRFAVEFVREPDAQLGYLAFD"
BASE COUNT         2044 a         1612 c         1526 g         1872 t
ORIGIN      
        1 ggcaataatt acaccttttg gtgcatttca tgccaggcaa taattacacc tttttgtgta
       61 tatggaaaga ttgaagaaag aaatacaaaa ttgttcgaaa tgtccgctga aggatgggcg
      121 gactaatgtt gtgtttggtt ctggcaacca gaacgctgat gttatgatca ttggtgaagc
      181 ccctggaaaa aacgaagatg agcagggcct tcccttcgta ggtaaagctg gtcaatatct
      241 tgatgaacta cttgctctcg caaatctcaa gcgagaagat gtctacatcg ctaatgtgct
      301 gaagtgcagg ccgccttcta accgcaatcc tcgtgccgaa gaaattcagg catgtgcgcc
      361 ttaccttcgc gaacaaaccg caaaggtaaa tccaaaagta attgtcacgc tgggcaactt
      421 cgccactcgt tttattctta aaaacgaaga aggcataacc tcgcttcacg gcaaacctgt
      481 ccaagcaggt aaattcactg ttttcccggt cttccaccca gcatctacaa tgtatgaccc
      541 caagaaaaaa tccgccctcg aaaaagattt ttctaaactt aaatcaataa taaataaata
      601 aaaataacaa ataatgaatt ctcgctgccc ccggccccaa aattttaggt gggatttgtg
      661 ttaaaatatt aaagttgtga cgtttatagg aggaaaaatg aaaaaaattc aacacaaagc
      721 taacacaagg tttggaatcg gaatgattct tgcgctgttg atagtgccag cgctaattat
      781 tggaatttgc actgttgcga acactcaaac gagttacgct aaagaagaag caattcaaaa
      841 tgggctaaat gattttttgg caactccgct caccgaagaa gagcaagccc ttgttgcatc
      901 taatgcagat aacgcaacag aaatcaagct ttatggaact gctacggagc aagatgtaaa
      961 tggtcaaata gttgcagtta aaaatttaac cataaaattc acgggcgtag cgccaaatgg
     1021 agattcgctt gacgtatcaa ctactacaaa cgaaaatggt gaatattcaa taacaattcc
     1081 tcaattttct aagggtgtat tgttttatgt tgatgaatac gcatcaactg agaatgctaa
     1141 ttacagtagt gcaagatgat gcatatatgt tgatattaaa gatactatta gtgatttcta
     1201 ttatgacttt tacgttgatt actcactaca agactgaaga aatttttatg ctgtagttaa
     1261 tcacatcgat ggtcttgatc aaccacaaac ttctcctgct ggaatattaa aggccactgc
     1321 tgaagttact ggtagaggaa ctcagactgt tgaagatact aaagttccat tacttatgaa
     1381 tgagtatcag tcggcggcgc ttgaatctaa cgctatagat agtgaatata acactcaaat
     1441 tcatgtatct ggcaagacaa ttacatatac atttactatc aacgatattg tatataaaga
     1501 aacgcttgag gcagaagaaa ctaattctga ttatgttttt gatggatggt ataagaagtc
     1561 cttgttgggc acaagagaca tggctgctaa cgaagtaaat aaacaacagc agcaatacaa
     1621 aaattatttt ggttattgta aagtaaatga cggagattat ttatgggatt ttgcacctga
     1681 ttcttttggt tttgaacaaa cggaagaaag ttcagctgat aatgcagcaa gtgccagtac
     1741 aaatggtgct ttttgtcttt atatagcgag gtttgtccca aagaccaacg ctcataaagt
     1801 tactttcaaa gtcacagacg aagcaaaatt caaccatggc tatattactg gctcaaatgg
     1861 aaacactata gattctgccg acgatgcatt aaaagaattc agttttaatg tcaacacaac
     1921 taatatggtc gcatacggat atgatgcttc gattcaaaaa cctgcatgat atatttcaga
     1981 tgacaagaga gaagaggcgc aactatcaaa atttgagtta aattgagttg gccataatac
     2041 ttcacagtca gatggttttt ataccgatgg ttttgtattt catccaactc cggaagctga
     2101 atacactttt agttcgtgaa aagttcttga taaagacggt aatgaaatta ctgttcctga
     2161 aagtggcatt ttgcctattg accaggactt gacttttgta gcttcgtttg ttaagacaag
     2221 aactttaaca ggaatagtta aagataatgc tggcaatcct ttggcacatg caagtgtttc
     2281 atttagagat tccaatggtt atagaactga agtccaagca gacgctgaag gtaaattcac
     2341 aatgaatgtt tccgctcttg ctggatattt gtctgcgggc aaaaacggtt acacaagcga
     2401 aaccattgaa attactgagg gaacaacaac ggtagatatt tcattaaaga ctcctgaaaa
     2461 tccagtttac ttggttggta atttattccg cttcgctgcc ggaggcggcg gaaatgctca
     2521 aaggcttaat gctgaggcaa gtaatagcta ccgatctatc tctgacgtaa caattaatta
     2581 ttctattaat gattatgaaa actacacata ttatgttgct ggacttcagg atgaagaaaa
     2641 tgccgaccca cattatgtaa gcgatttcaa aatgcctatt ccagaaggct cggcaggaca
     2701 gctaacattc actcatcaag gttatcaaac aatgagcttt cgtctaactt cagaaatgac
     2761 tcaaaaaact gaccctgaaa ctctaattaa tgttgccaag ctttcgattc ctgaagctga
     2821 tgtagaccct ggaaagcttg acccaagtta tgaaccacca gctattgttc ttgcatctgg
     2881 ttctgatcta aatgttttca tttcggttga cgacacaaat tcaggaaaca aaatctcatt
     2941 tactaatgaa caagaaggca ctcctgaact tttagactat acagccggaa tgcattatcg
     3001 cgcaggcgat aaaatttctg tagactttct ttctggcact ttaacaatta ctacagaagg
     3061 tgaaaccgaa gcagacacgg ctaagacaat cgtcacgcct gttcctaata aggaagcaaa
     3121 ttacgaattc ttcggatggg ttggctattc aagctttggc gagcgcatcg aattcatgag
     3181 cgaagacact ttgcaaaccg accttaacat ttatgcgcag ttcaaaaagg cggctcagct
     3241 aactccgctt gcattaagcg aaggccatga atcctatacg gaagagttct acagcaatga
     3301 gcttaagcat gtcggcgaag acaagccaat tgttgttacc aagttccagc ctgatccgct
     3361 tcgtcaatat gcgggcacta agtattctgt agttgataat cagattaagt ttgaatatga
     3421 cgaatcttgg gaaaatccag atatcggaga tgtttataag gagcaaacca ctttgacggc
     3481 aaatgccaag gatggttgga ccttcgatta ttggcttgtt aatggccagc gtgtagatga
     3541 tacagttact ccaattcaaa tttctacgga caatttaaag ttggatgttt tggctgtata
     3601 tacttcgcct gatgttcctc caactcctgt tgttgaccca acagcgggtg gaactgcaca
     3661 aactggcgat ggaattccag ctgctgtttt ggcgctttgc gtgttgttcc tcgctggcgc
     3721 tgcaattgcc gcaagacgtc aagggataaa gtttactaaa taatgagtta cgattctgca
     3781 tttggacaaa tgatgcacca cgtcttcggc ggcttcgaca tgtcgatttt cgccgcattt
     3841 ggatttgtcc aaaatgacat ttttacctgg ctggcacatg ccttcaccgc atttggccag
     3901 ccagagtttt gcatcatgct gtttataatg gggctggtca tgtgcgcatt caagcgcacg
     3961 cgcaaaatcg gcctcttgat aatatgcaca atcggactct ttttcatcac gaacaaccta
     4021 atcttgaaga acttgttttt caggcttcgt ccatataatg cgcttcaagg aaacgccgaa
     4081 tatttccagt gatacctcaa cgttggtgca atcgccgaaa cagaaacatg cttcccatct
     4141 gggcattcgt gctttgcatt ttcaatggcc acggcgctgt tcctctggat tcgaaacgac
     4201 ctcaaaaaat ccgaaggctg gatgctgttt atcccggcgc ttttcgtcgc gtgctcgcgc
     4261 atctacctaa tggttcatta tccgaccgac gtcatcgccg gcatcctcga aggcatcctc
     4321 ctcggcacca tagtttgata cgcgctcaag ctgatctttt tcgcgggcga aaagttcgcg
     4381 ccaagcttca ctgaaaagtg caccgaagct gacctggagc ctcgcctcga aaagaagctc
     4441 aatcgcaagc cggataccaa caaggttatt ggcatcattg tgctgcttgt catttgcttt
     4501 tgggccgcga gcttttcaag gctcatgatt gacatgaaca aggccgagca ctgttcgcat
     4561 cagggccctg actacatttg catgaacgag ggctatattg cgtctcaaga cgaagcgacc
     4621 ggccagtgaa aatattattg tgacctgcac gattaaatgc ctaacacgac cgacataaaa
     4681 aatgccccaa tcggagtcat cgattctggc atcggcggca tcaccgtttt gcgcgaactt
     4741 ttgcgcacct gtccgaccga gcacttcgtc tatattggcg acgatgcgaa ttcgccctac
     4801 ggcactcgga ccgccgaaga catccagcaa ctcagcctcg cgcttatcaa gcaccctaag
     4861 cttgtgcaca caaaagcgct ggtagttgca tgtaatacaa tcacggcagc cgcgcttgat
     4921 gatatcgctc aaaattacga aggccccgtc ttcggtgtga ttgagcctgg aatcgatgaa
     4981 gtcctcgcca ccacaaaaaa caatcacgtg ggcattcttg caacaacggc aactgtagaa
     5041 tcgggcgtct tcgaacgcgc gcttaagagc gctaacccgg acatcgaagt cacttcggtc
     5101 ggtgcgccaa agctccacga attcgccgaa aacaaccttg atcttgtcct cggcgcgcca
     5161 actcaagaaa ttaaggagtg tgtggttgag cacattgcgc ctttcatcga cgcaggttgc
     5221 gacactattt tgctcgcgtg cacgcacttt ccaccatacg cgtctctcat tcgagacatt
     5281 gtcggctcgg acgtaaacat catcgcgcca agcgttaata ccgccaaagc acttcgcacc
     5341 actttgcaag atttgaacct gaccaaccaa agttcagcaa accaaaccca cgcacacctc
     5401 aattatgaga taatttcaac cgcgggcaac gatgaaaaat tcgcaaagtt ttcggagcta
     5461 ttactaaaat aatgaaagtt ttattggcaa caaataatca aaacaaggcg cgcgaaattc
     5521 gcaatatcgt cgcaggtaga gatatcgaaa tctacacgct ttcggacctc aatcttgcct
     5581 ccgaccccga agaaaccggc cagaccttcg ctgaaaacgc agccatcaag gccgaagctg
     5641 ccctcgccga aatgaaggag aagggcttcg cgggttacgc ggtcgtggct gatgacagtg
     5701 gactttgcgt cgatgccctc gacggtggcc cgggcgttta ctccgctcga tatgccgaag
     5761 tcaacggcaa gccttgcact tataaagata acaacaagaa gcttctcaag gctctcaaaa
     5821 aaattccaat ggcggagcgc accgcccact tcgaaaccgt ggcggtcctt atcaacgaag
     5881 atggcactcg caccgaagcg gtcggccagc tcttcggcac aattggcaag cacgagcgcg
     5941 gccacaacgg cttcggctat gatccaattt tcatccccga aggcactgca atcacgctgg
     6001 ctgaactcag cgccaaagaa aaagacgaaa tttcacaccg cggccaagcc ttccgcaaac
     6061 tcttccaata aaattttcat aaattgtgct aatatactgc gcacgcgccc gtggctcaat
     6121 ggatagagca acagacttct aatctgtagg ttgcaggttc gactcctgcc gggcgcgcca
     6181 ttaattatgt taaacgaaat ttttcaaaat atagatccag tggcttttca agttggaggc
     6241 ttcagcgtta ggtgatacgg cattgcttac atcttgggtt ttgcctgcgc cagcattgtt
     6301 ttggtgcttg ttgccaggcg atggaaaatt cacgtcgagg ttgacaaagt cctcgcatgc
     6361 atcttatgcg gaattgccgg catcataatt ggtgcgaggc tgggctacgt gcttttctac
     6421 ggcaacggat attattgaac taacccactg aaaattcttg caatctacga aggcggcatg
     6481 agcttccacg gaggttttat cggcggcatt attggcggtt tctttggcgc taaaattatg
     6541 ggaatcaaat acctgcgctt gttggacctt gcttttatcg gtgttccgat cggccttttc
     6601 ttcggccgtt gcgcgaactt catcaacggc gaactttgag gcgcgccctg cgatttgccg
     6661 tgagcggtta acttcggagg ccatgcagga aatatgccaa ggcatccatc ccagctctac
     6721 gaagcaatcc tcgaaggtct ggtcctcctt gcaattatgc ttatcttggc tcgcaaactt
     6781 ccaccttaca aagaaggaac ttacttcgcg gttttctgca tcttctatgc agtctttcgc
     6841 ttcgcggttg agttcgtccg cgaacccgac gcccagctgg gctatctcgc cttcgactga
     6901 ctcacaatgg gccaactcct cagcatcccc cttttccttt taggaatcgg aatcctaatt
     6961 tgaaataaca aaaaataatc aaaaataata aataataaac tcccgctgcc cccggcccca
     7021 aaagtttctt tagaaggagg gaaaagggga ggtc
//
LOCUS       DBNCDC010000058         7052 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_5523_length_7052_cov_7.714878, whole genome shotgun sequence.
ACCESSION   DBNCDC010000058 DBNCDC010000000
VERSION     DBNCDC010000058.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7052)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7052
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5523_length_7052_cov_7.714878"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            104..1339
                     /gene="gltA"
                     /locus_tag="AC04VQ_04995"
     CDS             104..1339
                     /gene="gltA"
                     /locus_tag="AC04VQ_04995"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HIO9553506.1"
                     /translation="MAYNKSLEKNPMPSQEADVRNKNFNEVALGYTEEVALDEADRCL
                     GCKKPTCVEGCPVNINIPEFISHIKEKDYEGAYQEISKDSSLPAVCGRVCPQETQCEQ
                     LCVRGKKGDPVGIGRLERFVADWHNENVSDKPAKPNTNGKKVAIIGSGPAGLTCAGDL
                     ARKGYEVTVFEALHLAGGVLVYGIPEFRLPKAIVQKEIDTLKDLGVKVETDVVVGRTI
                     TIDELMKEKGFSAIFIGSGAGLPKFMRISGENFKGVYSANEFLTRINLMKAYKDDSST
                     PIMHPKRVAVVGGGNVAMDAARCSKRLGADVTIVYRRTDAELPARREEIEHAKEEGID
                     FHMLTSPIEIKGDDNFMVNSIVCQKMELGEPDDSGRRRPVPIEGKTETLDVDCVIMAL
                     GTNPNPLIKDTTDGLEVQK"
     gene            1625..2017
                     /locus_tag="AC04VQ_05000"
     CDS             1625..2017
                     /locus_tag="AC04VQ_05000"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIO9553507.1"
                     /translation="MRYKAHLPYKEKTARELIFKAFENENAEEVDKFIDKFYEENLEE
                     TVREPLLRIAEELRQGGMELVILSGAFTSTLGSFAKRHDFMHIVGTEMEVDNQGNYTG
                     KVDGECVAGEEKIKRLYNYADEHFGKDN"
     gene            2173..2880
                     /gene="pyrH"
                     /locus_tag="AC04VQ_05005"
                     /pseudo
     CDS             2173..2880
                     /gene="pyrH"
                     /locus_tag="AC04VQ_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012899.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
     gene            2880..3107
                     /locus_tag="AC04VQ_05010"
     CDS             2880..3107
                     /locus_tag="AC04VQ_05010"
                     /inference="COORDINATES: protein motif:HMM:NF014069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIO9553508.1"
                     /translation="MLDIFDFESRKISKNAEERNPYTDEFFESLLADLKERVDEERYT
                     HTLGVIEMADILCDVYGIDKKKARLAAAIHD"
     gene            3132..3503
                     /locus_tag="AC04VQ_05015"
     CDS             3132..3503
                     /locus_tag="AC04VQ_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553509.1"
                     /translation="MRKRIFAVGANSYASEDIIDKMPKVMHGATAAFALGIEYPNIPS
                     DVLRAVINHTTANVRMSRLDMVIYIADALDPSRDYPEYDELVELIGKICLEDLFCRVF
                     AESTIAVIKKGKPLHPKTVEV"
     gene            3551..3829
                     /locus_tag="AC04VQ_05020"
     CDS             3551..3829
                     /locus_tag="AC04VQ_05020"
                     /inference="COORDINATES: protein motif:HMM:NF013980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjP-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HIO9553510.1"
                     /translation="MEKIVIENIPISFTRKKIRNINIRISDPNGEVKVSAPYSISMRR
                     VEKFISERKDWILAAQQKVIERSKNKSKFHTQLFDSCTNEKEIKEKKK"
     gene            3950..4102
                     /locus_tag="AC04VQ_05025"
                     /pseudo
     CDS             3950..4102
                     /locus_tag="AC04VQ_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013980.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M48 metallopeptidase family protein"
     gene            4125..5030
                     /gene="xerD"
                     /locus_tag="AC04VQ_05030"
     CDS             4125..5030
                     /gene="xerD"
                     /locus_tag="AC04VQ_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIO9553511.1"
                     /translation="MDNDLSGFSRNYINYLRIEQGVSNNTIDAYSRDLAHFEKFLKAC
                     NPPKMINTAKREDIVNYEKELNPYMKATSIKRKVSAIKGMYKFLMREGDVKNNPAASI
                     PLPKPEQKLPDVLSIDKICKLLDLMPDTTSAALRNKAIMEVLYGCGLRVSELCGLNLQ
                     DCALSDGFLMVHGKGGKDRISPISGTAAQVLLDYIENARPEFDRRAKQSSSAVFLNAH
                     GGRLSRQSVHTIVKESGEAIHVKNLHPHTLRHSYATHMLEGGADLRVIQDILGHSDIS
                     TTQIYTHVSNSHIKEEYLAAHPRAK"
     gene            complement(5023..5700)
                     /locus_tag="AC04VQ_05035"
     CDS             complement(5023..5700)
                     /locus_tag="AC04VQ_05035"
                     /EC_number="6.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013094.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_function: GO:0061503 - tRNA threonylcarbamoyladenosine
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HIO9553512.1"
                     /translation="MSNVNEKYSRLEAIYSEEQLKKFKNSHIAVIGLGGVGSFCASAL
                     ARSGVGNLTIIDFDNIEASNINRQLFATENTIGKLKTDVAENYLHEINSSLKIITIPE
                     KVNSTNLNKIKSVDYIVDAIDDVPAKIEIVKFAQENSVRLISCMGTAMRKDATLLRFD
                     DIYKTSVCPLCKNVRKLAKENGIEKLEVLFSTEPAIKSQNGELGSTTYLPPIAGLMLA
                     GKVLESI"
     gene            complement(5701..5880)
                     /locus_tag="AC04VQ_05040"
     CDS             complement(5701..5880)
                     /locus_tag="AC04VQ_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553513.1"
                     /translation="MRVVPWTINSVEDMEKALAMECDGFVTDYGYLARELLIEKGASL
                     PKAYSFNSPYHVDLD"
     gene            complement(6139..6783)
                     /locus_tag="AC04VQ_05045"
     CDS             complement(6139..6783)
                     /locus_tag="AC04VQ_05045"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HIO9553514.1"
                     /translation="MSKVSKITISSLIAIVTAVLIIIPITSCTTTTPTYEKYFDLQFE
                     RGGRAAMPENTLYAFTYAIEQGATTIETQLQMTKDGVVVTSHSPLLNPDITMDKKGNY
                     VTGNEDIRTMNYAEVEQYNVGHIKKDSEYYKEFGKTQYEVDAKIPTLEELLKLVQDSG
                     NKEIMLNLETELYPDPALGALYQNNVDKNVFVNNILNLVKKYNMENRIIIQSFD"
     gene            complement(6804..6878)
                     /locus_tag="AC04VQ_05050"
     tRNA            complement(6804..6878)
                     /locus_tag="AC04VQ_05050"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6844..6846),aa:Thr,seq:tgt)
BASE COUNT         2284 a         1103 c         1449 g         2216 t
ORIGIN      
        1 ttaattgatt ttgacgaagt tatggcccgc gctacaacat acggtgaatg agaaaagaaa
       61 cgtcacgaag agacgtgtaa cttatataag aaggaggcat aaaatggctt acaacaaaag
      121 tttagagaaa aatccgatgc cttctcaaga ggctgacgtt cgtaataaaa acttcaacga
      181 ggttgctctt ggatataccg aagaggttgc tttggacgaa gctgaccgct gtcttggttg
      241 caagaaacca acttgtgttg aaggatgtcc tgtaaacatc aatattcctg aatttatttc
      301 tcatattaaa gagaaagatt acgaaggagc ttatcaagaa atttctaagg atagttcttt
      361 gcctgctgtt tgtggtcgtg tttgtccaca ggaaactcaa tgtgagcaac tttgcgttcg
      421 tggcaagaaa ggtgatcctg taggaattgg tagactggag cgcttcgttg ctgattggca
      481 taatgaaaat gttagcgata agcctgctaa gccaaacact aatggcaaga aagttgcaat
      541 tattggttct ggtcctgctg gtcttacatg tgcgggtgat cttgctcgta aaggttacga
      601 agtaacagtc ttcgaagctt tacatttggc tggcggtgtt ttggtttatg gtattccgga
      661 atttcgtctt ccaaaagcta tcgttcaaaa agaaattgat actctaaaag atttaggtgt
      721 taaagttgag accgatgttg ttgttggtcg tacgataaca attgatgaac ttatgaaaga
      781 aaaaggtttt agcgctatat ttattggaag tggcgctggt cttccgaagt tcatgagaat
      841 ttctggagaa aactttaaag gtgtttattc tgcaaacgaa tttttgacac gtatcaattt
      901 gatgaaagcc tacaaggacg attcttctac tccaattatg catccaaaac gtgttgctgt
      961 tgttggtggt ggaaatgttg ctatggatgc ggcgcgttgt tctaagcgtt taggtgctga
     1021 cgttactatt gtataccgtc gtactgatgc tgaacttcct gctcgtcgtg aagaaattga
     1081 gcatgcaaaa gaagagggta ttgacttcca tatgttaaca agcccaattg aaattaaggg
     1141 cgatgacaac ttcatggtta atagtattgt ttgtcaaaag atggaacttg gtgagccaga
     1201 tgattctggc cgtcgccgtc ctgttccaat tgaaggcaag acagaaactt tagatgttga
     1261 ttgcgtaatt atggctttgg gtacaaatcc aaatccatta attaaagaca caacagatgg
     1321 tttagaggtt caaaaatgag gtggaattat tgttgcggag gaaactggag caacttcgcg
     1381 cgaaggtgtt tatgccggag gcgatgctgt aactggtgct gcaactgtaa ttttagctat
     1441 gggagctgga aagactgccg cacaggctat tgacgaatac ttaaattaaa tgaagactaa
     1501 attagcagtt tttgattttg atggcacttt gattccggag caatctggac atcagctgtc
     1561 attagatttg gttcataata aacaattaag taagtggaca gccactaagc ttattttgtg
     1621 aggtattcgt tataaggctc atcttcctta taaagaaaaa actgccagag aattaatttt
     1681 taaagcattt gaaaatgaaa atgctgaaga agtcgataag ttcattgata aattttatga
     1741 ggaaaattta gaagagaccg ttcgtgaacc attgcttcgt attgccgaag aacttcgtca
     1801 aggcggaatg gaattggtta ttctatctgg ggcttttact tcgactttag gaagttttgc
     1861 caagcgtcat gattttatgc atattgttgg aactgaaatg gaagttgaca accaaggcaa
     1921 ttacactgga aaagttgacg gcgaatgcgt tgctggtgaa gaaaaaatta aaaggttgta
     1981 taattatgcc gacgagcatt ttggaaaaga taattgagaa attgaatatg ctcttgccga
     2041 tcattatacc gatagagatt tattagattt agcaaataaa gtttatgctg ttgatccaga
     2101 ttctactttg agacgttatg caaaaaagaa cggttgagaa attattgaat ttgaaaagcc
     2161 taaggagaaa taatggctga ttttaaatac aaaagagttg ttttaaaact ttctggcgaa
     2221 gcacttgctg ggaaaaatgg cgagagcttc gatattgctc ttatggaaga aatttcaaat
     2281 gagattagca aacttatatc tggcggagtt gaagttgctg ttgttattgg tggcggaaat
     2341 attattcgtg gagcaagtca gacgtgaatg aatcgcgaaa atgctgatta tatgggaatg
     2401 acagcaacag ttataaattc aatggcgttg gcagaagtac ttcgtaagaa taatttgaaa
     2461 gcgaaagtat tttctgcaat tccaatggag gttgtttgcg aaacatataa tccatcagtt
     2521 gctgacgaat atttaaaacg tggcgaagtt gtagttttag gtggtggaac aggtaaacct
     2581 tatgttacta ctgacactac ttcaactctt cgtgctaaag aacttaactg tgattgtgtt
     2641 ttgaaagcta caaaagttga tggtgtttat gatgatgatc cagtgaaaaa tcctgacgct
     2701 aagcgatttg ataaaatttc ttttgatgaa tgcctggagc gtggtcttaa agttatggac
     2761 gaaaaagcat ttaagatggc taaggacgaa aatattcata taattgtttt taatatgttt
     2821 gagaaagata atattgtaaa agtattgcaa ggcgaagatg taggaacggt agtttcgtaa
     2881 tgttagatat ttttgatttt gaaagtagaa aaattagtaa gaacgcagaa gagcgtaatc
     2941 cttacacgga tgaatttttt gaaagtcttc ttgcggattt aaaagaacgt gtcgatgaag
     3001 agcgctacac tcatactctt ggcgtaattg aaatggcaga tattttatgt gatgtttatg
     3061 gcatcgataa aaagaaagct cgtcttgcgg ctgcaattca tgattgagat aaatgttata
     3121 acgacgaaga aatgcgtaag cgaatttttg cagtaggtgc aaattcttat gcatcagaag
     3181 atattataga taaaatgccg aaggtcatgc atggtgcaac cgctgctttt gctcttggaa
     3241 ttgaatatcc taatattcca tccgatgttt tgcgtgctgt tattaatcac acaactgcaa
     3301 atgttcgcat gtcaagattg gatatggtta tctacattgc tgatgcgctt gatccaagta
     3361 gagattaccc agaatatgat gaattggttg agcttatagg taagatatgt cttgaagatt
     3421 tgttttgtcg agtcttcgcc gagtcaacca ttgcagttat aaagaagggt aaaccacttc
     3481 atccaaaaac tgttgaagta tgaaatgcaa atctagaaat gtacaatgca agacatgcta
     3541 acgattaggc atggaaaaaa ttgttattga aaatatccca atttcattta caagaaaaaa
     3601 aattcgaaac attaatatcc gcattagtga tccaaatggc gaagtaaagg tttctgcgcc
     3661 atattcaatt agtatgagaa gagttgaaaa gtttattagt gaaagaaaag attggatttt
     3721 ggctgcgcag caaaaagtta ttgagagatc aaagaataaa agtaaatttc atacgcaatt
     3781 atttgattca tgcactaacg aaaaagaaat taaagaaaag aaaaaatgat ttgagcaaga
     3841 attattgcaa caagctcaac cttattttga taaatgagaa aacttaacag gattatctcc
     3901 agaaaatatt gtgattcgat ggcctaaaac tttatgagga agttgcgata aacaaaaaaa
     3961 gaagatcatg cttaatggac aacttgttgc catggataaa aaatatttag attatgttgt
     4021 tttgcacgaa ttaactcatt taaaatattc taatcatgga gaagatttca aaaattttat
     4081 gacacaatac atgccaaatt gaaaagatgt gaggcgagag ttaaatggat aatgacttaa
     4141 gtggtttctc tcgaaattat attaactacc tgcgaataga gcaaggtgta tctaataaca
     4201 caattgatgc atattctcgc gatttagctc attttgaaaa gtttttaaaa gcttgcaatc
     4261 cgccaaagat gatcaatacc gcaaaacgtg aagatattgt taattatgaa aaagaactca
     4321 atccttatat gaaggcaaca agcattaaga gaaaagtttc tgcaattaag ggcatgtata
     4381 aattcttgat gcgagaaggt gatgtaaaga ataatcctgc ggcaagtatt ccattgccaa
     4441 aaccggagca aaagcttcct gatgttttaa gtatagataa aatttgtaag ttgcttgatt
     4501 tgatgccgga tacaacttcg gcggcgcttc gtaataaagc tattatggaa gttttgtatg
     4561 gttgcggctt gcgtgttagt gaactttgtg gtttaaattt gcaagattgc gcgcttagtg
     4621 atggattttt aatggttcat ggtaaaggcg gaaaagatag aatttcccca atttcaggaa
     4681 ctgccgcaca agttttgtta gattacattg aaaatgctcg accagaattt gatagaaggg
     4741 cgaagcaaag ttcttcggct gtgtttttga atgcacatgg tggtaggctt tcgcgtcaaa
     4801 gtgttcatac tatagttaaa gaaagcggcg aagccattca tgtaaaaaat ttgcacccac
     4861 acacacttcg tcattcatat gcaactcata tgttagaggg cggagcggat cttcgtgtca
     4921 ttcaagatat tttgggacat tcagatatat ccactacgca aatttatacg catgtttcaa
     4981 attcacatat caaggaagaa tatttggcgg ctcatccgcg cgctaaatag attcaagaac
     5041 ttttccagca agcataagac cagctattgg tggtaaatac gtagttgaac caagctcgcc
     5101 gttttgagat ttaattgccg gttcagtaga aaataaaact tctaactttt caattccatt
     5161 ttcttttgca agttttctca cattcttaca caaggggcac acgcttgttt tataaatatc
     5221 atcaaaacga agcaaagtag catcctttcg cattgccgtt cccatgcaag aaattagtcg
     5281 gacagaattt tcttgtgcga attttacaat ttcaattttt gcggggacat catcaattgc
     5341 atcaacaata taatctacag atttaatctt gtttagattt gtggagttta ctttttctgg
     5401 aattgtaatt atttttaaag aagaatttat ttcatgtaaa taattttctg caacatcagt
     5461 tttcaattta ccgatggtgt tttcagttgc aaaaagctgt ctattaatat tcgacgcttc
     5521 aatattatcg aagtcaatta tagttagatt tccaacaccg cttcttgcta atgccgaagc
     5581 acagaacgac ccaacaccac caagcccaat tacagcaata tgagaatttt tgaatttttt
     5641 taattgttct tcactatata tagcttctaa gcgagaatat ttttcattaa cattactcaa
     5701 ttaatccaaa tcaacatgat atggtgaatt aaatgaatag gcttttggta atgaagcacc
     5761 tttttcaatc aaaagttcgc gagctaaata tccatagtca gtaacaaagc catcgcattc
     5821 catagctaaa gctttttcca tatcttcaac tgagttaatt gtccatggaa caactcgcat
     5881 tcctcaatcg tgtgcttgta taatttgtgt tgttgtaatc tcatcaagtt gaggagaaac
     5941 tatatcgcag ccaagagcaa atgctatatc aatagcattg agttcaaaat cgtgaatatt
     6001 atatccagaa agccatggcg aaggttcttg tttttcaagt cagagattac caacacccag
     6061 ttcaggacct ccaacttctt cccataaagc agcagttaaa atatttggat tgaattcatc
     6121 aacaattgga aatacggatc aatcaaaaga ttgaataata atacgatttt ccatgttgta
     6181 tttctttaca agattaagaa tattattaac aaatacgttc ttatcaacat tgttttgata
     6241 aagagcacct aaagcaggat caggatataa ttcggtttca aggtttaaca taatttcttt
     6301 gtttccagaa tcttggacaa gctttaataa ctcttccaat gttggaattt tagcgtcaac
     6361 ttcgtattgt gttttgccaa attctttata gtattcagaa tcttttttaa tatgcccaac
     6421 attgtattgt tcaacttcag cgtaattcat agtgcggatg tcttcgttgc ctgttacata
     6481 attgcctttt ttgtccatag tgatatccgg atttagaagt ggactatgac ttgttacaac
     6541 aactccatct tttgtcattt gtagttgtgt ttcgattgtg gttgcacctt gttcaattgc
     6601 atatgtaaaa gcatatagtg tattttctgg cattgcagca cgtccacctc gctcaaattg
     6661 aagatcaaaa tatttctcat aagttggggt tgttgttgtg catgaagtaa taggaataat
     6721 aatcaataca gcagtaacaa ttgcaattag agaactgatt gtaatcttac ttactttaga
     6781 catggaatac tcctttctaa aagtggagcc agcgaaggga atcgaacccc caacccactg
     6841 attacaaatc agttgctcta ccattgagcc acgctggcat ccgttcgtgg tattttacag
     6901 gaaatagaaa aatctgcaac tatttttgta aaaattcgta aatattaacg cagcaaattt
     6961 tttgtttcgt gatatgataa gcgcatggca tttgttcacc tacataatca tacagaatat
     7021 agccttttag atgggcaaac acacgtcgaa ga
//
LOCUS       DBNCDC010000059         6990 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_5601_length_6990_cov_5.472675, whole genome shotgun sequence.
ACCESSION   DBNCDC010000059 DBNCDC010000000
VERSION     DBNCDC010000059.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6990)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5601_length_6990_cov_5.472675"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="AC04VQ_05055"
     CDS             complement(<1..268)
                     /locus_tag="AC04VQ_05055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013979775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIO9553515.1"
                     /translation="MHYDGSSCNGCDIEVLAALCPGFDAERFGVINTGNPKHADIFVV
                     TGSVNEQNKDVIKQIYDQMVEPKVVAACGTCACSGGVFSECYNVK"
     gene            complement(534..1265)
                     /locus_tag="AC04VQ_05060"
     CDS             complement(534..1265)
                     /locus_tag="AC04VQ_05060"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HIO9553516.1"
                     /translation="MVDYLSYFQYFGMELLINVGIVLACSVVFAILGTFFGCLFAGID
                     RKISAKMQGRVGPPILQPYYDVRKLFDKDRVSVNGSEKVYVTFALIFVFIAGGIFLSS
                     FVYYYLGSLTDGSNWLLCIFVLTLATMLFIMAAYSSRSPYAEIGGAREILQVVAYEPT
                     TMLMAVGLCLVTNSFSVQSVLMLDVPLITFLFPTFFCFLFILIIKFRKSPFDISMSHH
                     AHQEIVRGMTTEMSGPTLGKVEIMH"
     gene            complement(1278..1742)
                     /locus_tag="AC04VQ_05065"
     CDS             complement(1278..1742)
                     /locus_tag="AC04VQ_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553517.1"
                     /translation="MVVLVILCVATLPFISDFVVSSYLYDAFGVLGYQALYFMYSYGL
                     LGSPLSMDCVHVCILSLIVIALILFLRVGKSNKQKQANIYLAGASLDNDKRIYQGSMG
                     AKVEATSKNMYLNDIFGERSLAPFGAVLNIILFALAGIASVVLMTGIIPGVV"
     gene            complement(1827..3236)
                     /locus_tag="AC04VQ_05070"
                     /pseudo
     CDS             complement(1827..3236)
                     /locus_tag="AC04VQ_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit 5 family
                     protein"
     assembly_gap    3321..3420
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3573..3920)
                     /locus_tag="AC04VQ_05075"
                     /pseudo
     CDS             complement(3573..3920)
                     /locus_tag="AC04VQ_05075"
                     /inference="COORDINATES: protein motif:HMM:NF013334.8"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
     gene            complement(3920..4501)
                     /locus_tag="AC04VQ_05080"
     CDS             complement(3920..4501)
                     /locus_tag="AC04VQ_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012267.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIO9553518.1"
                     /translation="MLDGEAVELRPNGGILANKLTMQTAQPDIFTAGDVYTGPKFAID
                     AIAGGREAAISLHRFVHENCTLTIGRNRRDFIALDKQNVDFGEHSDVLRQKNELVKET
                     KPSFKKTVKTFTEEQAKIESARCLACGASHVDENKCIGCGVCTTKCVFDAITLSRKNP
                     ENSTMITSEEKLKVILPNMAKLKIKTAFKPKHF"
     gene            complement(4679..5563)
                     /locus_tag="AC04VQ_05085"
     CDS             complement(4679..5563)
                     /locus_tag="AC04VQ_05085"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIO9553519.1"
                     /translation="MNCYDTGTSPCKTACPAHIAIQGYLQLVKEGKYDEALELIKKDN
                     PLPAICGSICNKYCEAECSRGEVDEAVAIDDVKRFVAERDLNAKTQYIPKPIVPSLKG
                     KFDEKIAIVGAGPAGLSCAYFLALKGYKPTVFDKNEKPGGMLMYGIPPFKLEKDLVQA
                     EIEVLKKMGIEFKMGVEVGKDVTLDQLRKEGYKGFFLGVGCQLGRKPGVPGEDAEGVT
                     TAVDFLRECATKQKANIKGNVVVIGGGNVAIDTARSARRFTDGEITMVSLESAEEMPA
                     KPEEIEDAKADNIKIENS"
     gene            complement(5597..5983)
                     /locus_tag="AC04VQ_05090"
     CDS             complement(5597..5983)
                     /locus_tag="AC04VQ_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012267.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIO9553520.1"
                     /translation="MADYVVETNKGGEYITREEALEIFQKAEDNGFVHQITNIDGENK
                     IFAICNCNVNVCYALRTSQLFNTPYMSASAYKATVDKEKCVACGKCVESCPAGAVKLG
                     QKLCDKDGCTVEYPKIPMPYERKWGP"
     gene            complement(6002..6712)
                     /locus_tag="AC04VQ_05095"
     CDS             complement(6002..6712)
                     /locus_tag="AC04VQ_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553521.1"
                     /translation="MRTTKQKLDPELYPEYKYPEMDIKPDEVFRENVAALGKKITDRV
                     PQKLGLSKITQDDPEYYGLTAVLSDEEIEVAMKMNVRDPKTFEELVEITGKKPEELQP
                     LLDSMSKTGILEYNWENDKREKQYVLPMYVPGSAEFMNMNADVLKEHPQMGLFFERMT
                     RLPLEKITPYVKPGGSGIGMHVIPVEKAIEMENESIDLEHISHWLDKYDGKYAKSPCS
                     CRKSRLTHGEGCADDPEG"
BASE COUNT         2134 a         1526 c         1215 g         2015 t
ORIGIN      
        1 ctttgacgtt gtagcattca ctaaatacac caccagaaca agcgcaagtt ccgcatgcgg
       61 caactacttt tggctctacc atctgatcat aaatttgctt aataacatct ttgttttgct
      121 cgtttacact gcctgttaca acgaagatgt cagcatgctt tgggttacca gtattaataa
      181 caccaaaacg ctcagcatca aaaccagggc aaagtgcagc caagacttcg atgtcgcaac
      241 cattacagct tgagccgtcg taatgtatta ctcatggaga tttttttgca taagacataa
      301 actacctcct aaaccataaa catcaagaca acaagattta agccgcacaa aattaagcaa
      361 agtagccaag accatttaag catggcttgc cactttacac gtgcaaaatt gttgtcaatc
      421 caaatcatta acaaataaac taagataaca gctactacag cgcctaaaac tcacattgga
      481 tttgagcaaa cgaagaaaac tgctattcag cccatgaaca agaagttctc gtttcagtgc
      541 atgatttcaa ctttaccaag tgttggtccg ctcatttctg tagtcattcc tctaacgatt
      601 tcttgatgag catgatgaga catagaaatg tcaaatgggc tctttctgaa tttgataatc
      661 aaaataaaca agaaacagaa aaatgttggg aacaaaaatg taattagcgg aacatctaac
      721 atcaaaacag attgaacact aaatgaattt gtaaccaagc aaagaccaac ggccataagc
      781 attgtggttg gttcatatgc aacaacttgt aagatttctc ttgcgccacc aatttcagca
      841 taaggtgatc ttgagctata agctgccata atgaacagca tagttgcaag agttaaaaca
      901 aaaatgcaaa ggagccagtt gcttccgtct gttaaagagc caagataata atatacaaaa
      961 cttgataaga aaattcctcc agcaataaaa acaaagatca atgcgaaggt aacgtatact
     1021 ttttctgacc catttacaga aactctgtct ttgtcaaata atttacgaac atcataatat
     1081 ggctgcagga ttggaggtcc aactcttccc tgcatcttcg cgctaatttt tctgtcgatt
     1141 ccagcaaaaa gacatccaaa aaaagtgcct aaaatagcga aaacaacaga acaagcaaga
     1201 acaataccta cgttaattag gagttccatt ccgaaatatt ggaaatatga taaataatca
     1261 accatctcaa acctccttta aactacacca ggtattatgc cggtcattaa aacaaccgat
     1321 gcaattcctg ctaatgcgaa caatataata tttagaacgg cgccaaatgg agctaagctt
     1381 cgttcaccaa agatatcatt tagatacatg tttttagatg tagcttcgac ttttgcaccc
     1441 atagagcctt gataaattct tttgtcgttg tcaagcgaag caccagctaa atagatatta
     1501 gcttgctttt gcttgttgct ctttccaact cttaggaata ggattagggc aattactatc
     1561 aaagacaaaa tgcaaacatg aacacagtcc atcgaaagtg gtgaacctaa aagtccatat
     1621 gaatacatga aatataaggc ctggtaacca aggacgccaa atgcgtcata taaataagag
     1681 ctaacaacaa aatcagaaat gaatggaagt gttgcaacac atagtataac aagaacaacc
     1741 attgtaattt gcgaaattca ttcgcttctg tgaacagttt tttcaacatt gtccttattg
     1801 gcgataattc ctgttaactt ccccattcat tttgcccaga acataaatgt agctgcactt
     1861 ccaaatgcaa gaatcaatat caaagcaatt tgatttgcat ttacgaaaga aacaagagca
     1921 gcccacttag aaataagcat accgaatgga gcgataaaca tcgccatcat gccaaccaac
     1981 attagtcttg caaggcgtgg catgcgaacg aaaagagcgt ccatgtcttc gatatttctg
     2041 cttccgatgt gatgctctgc agttccaaca caaaggaaca acaaactttt tgcacatgca
     2101 tggaacaaaa tcaaaaacat cgcagctcat acagcatatg gagacccaac gcctgcgcaa
     2161 gctgtgatca agcccaagtt agcgattgta gaatacgcca acactctctt agcatttgtt
     2221 tgtgaaattg ccaagaatga gcaaatcagg aacgtgacac caccaacaag catgaccata
     2281 atagcaggtg cagctgtgat tgggctttga gttaagctta cactgtaaaa tactggagaa
     2341 agctttacta acaaaaatac accagcctta accatggttg atgagtgcag caatgccgaa
     2401 gttggagttg gcgcaaccat agcaccaagc aaccaagttt ggaaaggcat ttgagccgcc
     2461 ttcgtaattc ctgagaatgc taacaagcat aaaggcaatg cgactaaagc agtgtttgct
     2521 ataccagctg aaatgaactc gtttaatgcg agagtgttaa attgagttgc acttaaatat
     2581 aaggcagctg caaatgtgat tccgccaagc aagttcatgt taatttgtct gaaagcattg
     2641 cgaattgctt cgttagtttt ggtgtagcca attaacaaga acgaacacaa agttgttact
     2701 tcccaaccgg taaacatcca gatcatgttg ttcgagaaca caatcataaa cattgccgac
     2761 agaaacaaga acatcaatgc gaagaacctg ttagagcggt ctttttcggc aggtcttaag
     2821 tgcttattgt gatcgctcat ataaccaaga gcataaacac aaatgccagt gccaattaca
     2881 ccaataataa atgtcattag caatgtgaat gaatcgatgt agagattctc ttgaacttcg
     2941 ataccaggag caacagtaaa ctcgacgaat agcgagcctg ctacctgaat aactgcaagc
     3001 acaattgcta atgtgttttt gtacttaatg ccaaaagcta aaattgttgc agcaatcact
     3061 acacttgcgg ccatacatat ataatgcatg gcatgaacgg cttcgtcggc cattgaaatc
     3121 acagtgtaag acgctccaat gtttagcgca caaagtacaa tggacccgat tgcaataatt
     3181 cctccaaaaa aataaactgc gatttttcta acaatgccat cctttaaaaa cagcatgaga
     3241 atcgcagcta aaattggaaa gaataagagc ccatttacca aatactgaga aatgttttcc
     3301 acgttgttag acctcctata nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 agagcccatt taccaaatac tgagaaatgt tttccacgtt gttagacctc ctataattcc
     3481 tcgattttta caacactcga attataacaa aagtgcaaat aaaacaaaca attatttatt
     3541 aagatttcag ggaagaattt tgggcaacgc atttactcgt caatttcgat ttctttaatg
     3601 ttgaattcgt tgccgcaata taaataggta ttttcagatc cgcaatttgg acagattttt
     3661 gcatgtttta cggtgtcata tttttgctta cagtcttcac aatagcttag agcttttagt
     3721 ttctcaattt ccaaaacaca ttccttgaat aatgggcgct tcgcgcaagc tcagcttcag
     3781 caatcgtaca tgtattctgg aataactgtt gatacttccc caagctcgag gactactttt
     3841 gataccctat taatattgcg gtccttaacc acctcttcga ggcgatcaag gaccgtgaat
     3901 gtaacaccaa gttcgtgcat tagaaatgtt taggcttgaa agcagttttg attttaagtt
     3961 tagccatgtt tggcaatata accttaagct tctcttcgga ggtaatcatt gttgaatttt
     4021 cagggttctt cctgcttaaa gtaatagcat cgaagacaca cttcgttgtg caaacgccac
     4081 aaccaatgca tttattctcg tctacgtgac tagctccgca agctaaacaa cgcgcacttt
     4141 caatcttggc ttgttcctca gtaaaagttt ttacagtttt cttgaatgac ggctttgttt
     4201 ctttaacaag ctcgtttttc tgacgcaaaa catcgctatg ctcaccaaaa tcgacatttt
     4261 gcttgtctag tgcaataaag tcacgtctgt tacgtccaat tgtaagagta cagttttcgt
     4321 ggacgaatct atgcaatgag attgcagctt cgcgaccacc tgcaatagca tcaattgcaa
     4381 attttggacc agtgtagaca tctccagcag tgaatatgtc aggctgagct gtttgcatcg
     4441 ttaacttatt agctaaaatt ccgccgttag gacgaagttc aacagcttcg ccatctagca
     4501 tcttacctca atcgataccc tgtccaatac taaagattag tctattgcaa gcaattgcca
     4561 tggtgtcgtt ttcgtcatat tgaggcgaga atctatgatt ttcgtcgaag actcgagtac
     4621 atttcttgag tacaattcct attactttgc catctttaac ctggacctct ttaggacctc
     4681 aactgttctc aatcttgatg ttatctgctt tagcatcttc tatttcttca ggtttggcag
     4741 gcatttcttc agcactttca agagaaacca tcgtaatttc tccatcagta aatcggcgag
     4801 cgcttcgagc tgtgtcaatt gcgacatttc ctccgccaat aactacaaca tttcctttaa
     4861 tattagcttt ttgttttgtt gcacactcgc gcaagaaatc aacagctgta gttacacctt
     4921 cggcgtcctc gccaggaaca ccaggtttac ggcccaattg acatccaaca cccaagaaga
     4981 aacctttata gccttcttta cgaagttggt ccaaagttac gtccttacct acttcaacac
     5041 ccatcttaaa ttcaatgccc attttcttga gtacttcaat ttcagcttgc acaagatctt
     5101 tttcaagctt aaatggtgga atgccataca ttaacattcc gccaggtttt tcattcttgt
     5161 caaagacagt tggcttatag ccctttaggg ctaaaaagta agcacatgac agtcccgcag
     5221 gaccagcacc aacaatagca atcttctcgt caaacttacc ttttaaactt ggaacaatag
     5281 gcttaggtat atactgagtt tttgcgttaa ggtcgcgttc cgcaacaaat ctcttaacat
     5341 catcaatggc aactgcttca tcaacttcgc ctcttgagca ttctgcttcg caatatttat
     5401 tacaaatgct cccgcaaatc gctggaagtg gattgtcttt cttaatcagc tcgagagctt
     5461 cgtcgtactt accttctttt acaagttgca aataaccctg tattgcgata tgtgcaggac
     5521 aagcagtttt gcaagggctt gttcctgtgt catagcaatt aattctatta gtatcgcgat
     5581 aattatgctc tcacattcaa ggaccccact tacgttcgta aggcatcgga atcttcgggt
     5641 attcgacggt acagccatct ttgtcgcaaa gcttttgtcc taatttaacc gctcctgcag
     5701 gacaagactc tacacattta ccacaagcaa cgcatttttc cttatcaaca gttgctttat
     5761 aagcacttgc tgacatatat ggcgtattaa ataattgcga agtgcgaagc gcataacaca
     5821 cgtttacatt gcaattgcaa atcgcgaaaa tcttgttttc gccatcaatg ttagtaattt
     5881 gatgaacaaa gccattatct tcagctttct ggaagatttc aagcgcttct tcacgagtga
     5941 tgtattctcc acctttgttt gtctctacaa cgtagtccgc catatcgcca atggcaatgc
     6001 atcatccttc aggatcatct gcacaacctt cgccatgagt caatctactc ttacgacaag
     6061 aacatggaga ctttgcatat ttgccatcat atttatctag ccaatgagaa atatgctcta
     6121 aatcaatgga ctcattctcc atctcaatag ccttctcaac tggaattaca tgcattccaa
     6181 taccagaccc accaggtttt acataaggag taattttttc aagcggaaga cgagtcatcc
     6241 tctcgaaaaa cagacccatc tgaggatgtt cctttaagac atccgcattc atattcataa
     6301 actcagcaga acctggaaca tacattggaa gcacatattg cttctctcgt ttgtcattct
     6361 cccagttgta ttcaagaatg cctgtttttg acatactatc gagtaatggc tgcaactcct
     6421 caggcttttt ccctgtaatc tcaacaagtt cttcaaaggt cttagggtcg cgaacattca
     6481 tcttcatcgc aacttcgatt tcctcatcgc taagaacagc agtcaaacca taatactctg
     6541 gatcgtcttg agtaatcttt gatagcccca gcttttgcgg aaccctatca gtaatcttct
     6601 tacctaaagc agctacattt tcacgaaaaa cctcatctgg ctttatatcc atttccgggt
     6661 atttatactc tgggtataat tccggatcaa gtttttgctt ggttgtacgc atgtaagtcc
     6721 tttctaataa ttagtgaata attttgagtg gtatttattt gagttgtcgt atggtatggg
     6781 aaggtttccg cgggttttgc ccgcgcgcag ttccgcagcg aagcgaggac tgtttgagca
     6841 cgggcgatgc cccggaaacc aaatgcccat acatttaact actcaaaatt attcttcact
     6901 tttattcctt gcgagccaaa gcctctttgt aatccttagt atcagtaaag atttcatgtg
     6961 tgtaaggatt tagcttttgc ttcttcgcac
//
LOCUS       DBNCDC010000060         6979 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_5625_length_6979_cov_9.287984, whole genome shotgun sequence.
ACCESSION   DBNCDC010000060 DBNCDC010000000
VERSION     DBNCDC010000060.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 6979)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6979)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6979
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5625_length_6979_cov_9.287984"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            202..522
                     /locus_tag="AC04VQ_05100"
     CDS             202..522
                     /locus_tag="AC04VQ_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553522.1"
                     /translation="MNNTLTKKGINLTLIGVLFYAINVVATNSSFIVAMGDFSDFEDA
                     LLIIDSITDIIGVAAIVLIILGTAKLIKVCSTFKLVIPLVIVGILCTIAVDLVRDTIA
                     YQLT"
     gene            860..1192
                     /locus_tag="AC04VQ_05105"
     CDS             860..1192
                     /locus_tag="AC04VQ_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553523.1"
                     /translation="MYKEKKKFGIVLLSIFVAIFVISISMIGVNKALAVDEEEVDEEE
                     ITPEQFYDMDDPDEPNLGGSNSNGSPKPVWMIGHRANNRGNVDKALKQGANGVEIDIR
                     RKDGDQSK"
     gene            1229..1390
                     /locus_tag="AC04VQ_05110"
     CDS             1229..1390
                     /locus_tag="AC04VQ_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553524.1"
                     /translation="MSLEEYFSSEIRDQKKRYDASVKNKVKDGFCVLFLDIKTPDKDL
                     PDLQRQVRG"
     gene            complement(1886..2092)
                     /locus_tag="AC04VQ_05115"
     CDS             complement(1886..2092)
                     /locus_tag="AC04VQ_05115"
                     /inference="COORDINATES: protein motif:HMM:NF014731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HIO9553525.1"
                     /translation="MVLVAIGVVYGDIATSPMYVMKSIVAGNGGIDSIIKKEVEHFAQ
                     PLFAFIKLLLFAWCFSSIFKRIII"
     gene            2281..2514
                     /locus_tag="AC04VQ_05120"
     CDS             2281..2514
                     /locus_tag="AC04VQ_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553526.1"
                     /translation="MFKAIKENSKVVRVALITSLSAIALIFMMTLASCGIFQDLSKFV
                     NVFAGNATVGHTSPCATTPFGMIKVGPESGNMD"
     gene            2530..2832
                     /locus_tag="AC04VQ_05125"
                     /pseudo
     CDS             2530..2832
                     /locus_tag="AC04VQ_05125"
                     /inference="COORDINATES: protein motif:HMM:NF037611.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3181..4434
                     /locus_tag="AC04VQ_05130"
                     /pseudo
     CDS             3181..4434
                     /locus_tag="AC04VQ_05130"
                     /inference="COORDINATES: protein motif:HMM:NF019583.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
     gene            complement(4529..5548)
                     /locus_tag="AC04VQ_05135"
                     /pseudo
     CDS             complement(4529..5548)
                     /locus_tag="AC04VQ_05135"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            complement(5567..5737)
                     /locus_tag="AC04VQ_05140"
     CDS             complement(5567..5737)
                     /locus_tag="AC04VQ_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553527.1"
                     /translation="MGITDDTPDMVEGLSYYVRDDEGHITGNCFEGPHFCMVMRHDDE
                     IPDDAIESEFER"
     gene            complement(5762..6196)
                     /locus_tag="AC04VQ_05145"
     CDS             complement(5762..6196)
                     /locus_tag="AC04VQ_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553528.1"
                     /translation="MQVDKIIKNAKVFTSDKDQLDASAFAVKDGKFVYVGDEAGLDDF
                     TGEEIDLAGKFVMPGIIDAHVHLPVCVGALSLGDMHFVPGSSKDECIQFMKDVVNSSD
                     EVRYTFLITLASLAGEMLTQEDLNTVSSEKEIFVVESEMHSS"
     gene            complement(6213..6512)
                     /locus_tag="AC04VQ_05150"
     CDS             complement(6213..6512)
                     /locus_tag="AC04VQ_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIO9553529.1"
                     /translation="MEGGIRRIFDEHSNLSEVEKLYEMVPTEAEAMSIDEMLLGYTIN
                     GAKQLKIEDKKGSIEVGKDADFLIFDEDLTTRDAVGLSYVRPQEVWFAGELIHKK"
     gene            complement(6693..>6979)
                     /locus_tag="AC04VQ_05155"
     CDS             complement(6693..>6979)
                     /locus_tag="AC04VQ_05155"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIO9553530.1"
                     /translation="VEPYADTGTVGGRLLDKEQYAYLLKKLNKEGLDLHVHCVGEAAS
                     KAVLDGVELARKELGDDYHVQVTCAHLEIQCDEDLPRFAELSVIANYTPW"
BASE COUNT         2328 a         1263 c         1210 g         2178 t
ORIGIN      
        1 cctaaaaatg taattttatt catctttcct cctgcatgga tttatcctta ttttgacata
       61 acacattgta ccagactata gcagattcaa ttcccgtatc acgttaaatt attaattcaa
      121 aattttctgt gtatactttt aaacatatag gtaaaagaat gtatagtata attaacttat
      181 caattcatag aaaggggtct tatgaataat acactaacaa aaaaaggaat caatttaaca
      241 ttaataggcg tgctttttta tgcaattaac gtagttgcaa ctaatagctc gtttattgta
      301 gctatgggcg atttttcgga ctttgaagat gcgttattaa ttatcgattc cataacagac
      361 attattggag ttgcagctat tgtgctaatt attttgggta cggctaaatt aataaaggtt
      421 tgctcaactt tcaaattagt aattccttta gttatcgtag gcatcctttg tacaattgcc
      481 gttgatcttg ttagagatac aatcgcatac caattaactt gagttatgtg actagacatt
      541 gcttctacaa ttattagctg cttgtttatt actatgcttt gtgttggctt atcaaagctc
      601 tataaagaaa acaaaaatga aaagcgagca aagaaagcaa ttattgtaat agttttttac
      661 attattcttt ctattatctc tgtattgcta atgcaatttg taactatatt cagcggtcat
      721 atatgagata gcgaattatt agacatttta attgttggtt ggtttgctgt tttgttgaat
      781 attgttgcta acctgatagt aattctatat ttaaacaaag ccaaaaaaga attaggaaaa
      841 taagttagga ggtatgatta tgtataaaga aaaaaagaaa ttcggaattg tattactaag
      901 catttttgtt gcaatttttg taattagcat ctcaatgata ggcgtaaata aagctttggc
      961 agttgacgaa gaagaagtcg acgaagagga aatcactcca gaacaatttt acgacatgga
     1021 tgatccagat gagccaaatc ttggtggatc aaattcgaat ggtagtccta agcctgtttg
     1081 gatgattggg cacagggcta ataatagagg aaatgttgat aaggccttaa agcaaggtgc
     1141 aaatggtgtt gaaattgata ttagacgtaa agatggagac caaagtaaat gaattattaa
     1201 ccatgatgca tatagaccta atttaagtat gtcattagaa gaatacttct cttctgaaat
     1261 ccgagaccaa aagaaaagat atgatgcgag cgtaaaaaat aaagtgaaag atgggttttg
     1321 tgtcttattt ttagatataa aaactccaga caaagacttg cctgaccttc aacgacaagt
     1381 tcgtggttga atgcaagagt atcaaatgga attttatgtt gtttatcaag tagaaacctt
     1441 tgaccaagct atgttgcctg gaggattatg ccaatgctgg tgtaatcatt gagagggagt
     1501 ttgcatggca tttgattgcg gaagaagagc taaaaatgta attaaatgag atgattggtg
     1561 taaacaacat tgacaaaatg gaatggcaca cagttggtat ggtaatggct ttggatttga
     1621 accattctga tggataaaag gaaggataaa tacaggttta agaaaggcta aagtatttcg
     1681 tgacagtaaa gatggggcca ctcaaaagat tgggacttga tccctaaaca ctaaaaaagc
     1741 cgttgttaat tcatttatta aatatgattg cgattgagct ttacttgaat ttggtggtgg
     1801 agatgtagct cctaacggtt catatggtct tataaatgct caaacagtgc ttaaggatta
     1861 tagtaacaag attcgtttag ctaatctaaa tgattatcct tttgaaaatg cttgaaaaac
     1921 accacgcaaa taaaagtaat tttataaatg caaaaagagg ttgagcaaaa tgttcaacct
     1981 ctttttttat aatagaatca ataccaccat tccctgccac tatagacttc attacataca
     2041 taggcgaagt tgcgatgtct ccatacacaa caccaatggc aactaaaacc atcgcccccg
     2101 aaattttttt gttttttaat taaagatgac attttttatg ggttatgcca taactataat
     2161 aatattttaa taaaattttg aaacaattaa tcaacaaacg ttattagaaa tatttaaaag
     2221 tttcttgtat aatatgcgaa tcaaaataaa tcaatagaca aagtattttt ggaggtcaaa
     2281 atgtttaaag ctatcaagga aaattcaaaa gttgttcgag ttgcactaat aacttcgtta
     2341 agtgctattg ctttaatttt tatgatgact ctggcaagtt gtggcatatt tcaggaccta
     2401 agcaagtttg taaatgtttt tgctggaaat gcaacagttg ggcatacttc gccatgtgca
     2461 acaactccat tcggtatgat taaagttggc ccagaaagtg gaaatatgga ttgaaagtat
     2521 tgctctggat atcaatatca cgataaggaa ctttatggct tttctcaaac aagactaaat
     2581 ggttgtggat gcgctgattt aggagatttg cacgtatttc catttagcgg cgagtgagca
     2641 aaaacagatt ataaaagttt ctacgataaa gaatctgaag ttggtgaacc cggatattac
     2701 tctgtccatc ttgacgatgc gcaagtaaaa gcggagatga gttgtactcc tcatgtttct
     2761 atagaaaagt ttacttatga tgatccaaca aacgcaaact taatgattga ctttcaaagt
     2821 ggaattgttt gaagcaaaaa aatgtttcaa aacagagttg ttgaaggcta tcaaaatttc
     2881 gaagacccat atcatattac aggctacaca tcagctgaag catgagtaga aagagattat
     2941 tactacgaca tagaatttga cactccatat acagttaaag tagaacttcc gcttcgtgat
     3001 gctaaagaaa aagcgcatcg ttatgttctg tcttttaatc ttgaaaacgg aaaacctcta
     3061 acggttaaaa tgtcattttc taaaacaagc atcgaaaacg ccaaaaacaa tattaagacc
     3121 gaacttccaa attgggattt aaatcaggtt aaaaatgatg cgaagacaag ctgaaacaat
     3181 atcttaaatc gtgtaagcat tattggtgat gatagccaaa aacagttatt ttattcatca
     3241 atgtatagat tatttgaaca gccagaaaat gttggtgatg caggaaaaag ccctgaatat
     3301 tccactcttt ctttgtgaga tacatacaga gcagcacatc ctttatatac aatcattggc
     3361 caagaaaatg ttaacgattt tgttaattca atgctagatg aatttgatag agaaaagcat
     3421 ttgccaatat ggtccctttg aggaaatgac aattattgta tgattgcaaa ccatgcagtt
     3481 cctgtaattg ttgatgcata tttaaaagga tatgatggtt tcgatgcaga gcgagcatac
     3541 caagcaatta aaacttctct cactgtaagc catagaaatt caaattgaga agtttatgac
     3601 aaattaggat acttcccaat cgatcaagtg gcaaataaag aagacgatgt gaaggatcaa
     3661 agcgtttcac gcacattaga aagtgcctat gatgattatt gtgcagctca aatggcacaa
     3721 aaacttggct acacagaaga ttacaatttc tttatgaagc gttcaaacta ctataaaaac
     3781 ttatttgacc caacaaataa attaatgcga ggcaaagata gtgtgggtaa ttggcgtaca
     3841 cctttcagtg gcttcgatta ctatatttct aaaaatgacg acgacacagt gagagacttt
     3901 accgaagcta attcttggca atacacatgg catgttcaac aagatgtaca aggtctaatt
     3961 gatttatttg gaggcaacga agaattttgc gcagctttag acaaattctt tacgctggat
     4021 gatgacctcg attcaaacat gattccatct gacgtaacag gattaattgg acaatattgc
     4081 caaggtgatg agcctagcca tcatgctgca tatctttata caattgcagg acagccatga
     4141 aagactcaag cgcttatcaa taaaattatt agaacacaat acagcaatgg tgttgatggc
     4201 ctttgtggaa atgaagattg cggtcaaatg tcagcatgat atatattctc tgtgctaggt
     4261 ttctaccctg ttaacccttg cggtggcgaa tacatcattg gcgcgccaca aattcaaggc
     4321 gcaagtattg accttggcaa cggcaagaca tttacggtaa caacaaacaa ttatggcaag
     4381 aataatatct atgttaaatc tgttactttc aacggaaaag aaattacaga ttgaaaaatt
     4441 acgcacgaag atattatgaa cggtggcgaa cttgtcttca caatgacaaa cacacctcaa
     4501 atgcattaaa atttcagctt gattaaagct attttacttg ttggccagta aaaaatactt
     4561 gctcaggttc aagatgactt aacctgtccg gcttgacgct ggttaaatct tcttcaaaga
     4621 taagaaaatc agcgtctttg ccaacttcaa ttgaaccttt tttgtcctct aggcaaagtt
     4681 gcgttgcatt attaattgtg tagcctaata acatttcttc aatagacatt gattcttctg
     4741 cagctggcca aggtgtgtcg ctaaaacctt catccatagt aatattcgta tttctgtcaa
     4801 tttttctagt aataccaact tcaattccaa gcatcggatt tcatgtgcta aagtctccaa
     4861 agcttgtagt atcacaagat caacaaactg ttgctccaga tttccataag cttttactgc
     4921 gatacatttt atctatgcga tctcccaaaa acttttttcc gccttcatat ccgccagcca
     4981 tacagcatcc gctatgccac caaggcgtaa agtttgccat aacaccaagc tcggcatatc
     5041 taggaatatc ttcatcgtta attaattcat tatgagcagt tgttactcta acacgaaagt
     5101 catcgcctag ctcatttttt accatttcta cagcattaag aattgttcta ttggaaccat
     5161 ctgcaacgca atgagcatga agattaaagc caagtttatt aagttttctt aacaagtctg
     5221 ctacttgatc ctcattaaaa agtctaccac cagaagtttt ggtatcctca tatggctcaa
     5281 tcatacaagc agtacgaata ttcatagttc catcaaacat aagctttaaa gtatttactt
     5341 taacatgctc ggtattgtat gtttcatgtt gacgaactaa ctcatcaatt gcaccttcgg
     5401 cttgagcagg atcgtaaatc atgtagctac cttcaatggt aattggcaac atcccgcgcc
     5461 tatccatatc gcatagaatt tttaagccac gttctgttag cgctgcagaa cctggagttc
     5521 cagcttcaaa aacagaagta acgccaattt ttttactatt ttctactcag cgttcgaatt
     5581 cactttcaat tgcatcatca ggtatttcat catcatgcct cataaccata caaaaatgag
     5641 gaccttcaaa acaatttcct gtaatatggc cttcatcatc acggacataa tagctaagcc
     5701 cttcaaccat atcaggagta tcgtcagtta ttcccatatt atttaatatt acagaatttg
     5761 ctcaactgct atgcatttct gattcaacaa caaatatctc tttttccgaa gacacagtgt
     5821 ttaaatcttc ttgtgttagc atctctcctg caagagaagc aagcgtaatt aaaaatgtat
     5881 acctaacttc atcagaagaa ttaacaacat ctttcatgaa ttgaatacat tcatctttgc
     5941 tagatccagg aacaaaatgc atatctccta aagataaagc accaacgcaa acaggaagat
     6001 gaacatgggc gtcaataatt ccaggcatta caaatttgcc agctaaatcg atttcttcgc
     6061 ctgtaaaatc atctagtccg gcttcatcac ctacataaac aaactttccg tccttaacag
     6121 caaaagcgct agcgtcgagt tgatctttat ctgacgtaaa aacctttgca ttttttatta
     6181 ttttatcaac ctgcattttg tcctccttat atctattttt tatgaattaa ttcgccagcg
     6241 aaccatacct cttgtggtct aacgtaactt agtccaactg catctcttgt agttaaatct
     6301 tcatcgaata ttaagaaatc agcatctttg ccaacttcaa ttgaaccttt tttatcttca
     6361 attttaagct gttttgctcc gtttatagta taaccaagaa gcatttcgtc gatcgacatt
     6421 gcttcagcct cagttggaac catctcatat aatttttcaa cttcgctcaa atttgaatgc
     6481 tcgtcgaaaa tgcgtctaat gccaccttct atacctaaat atggacttca gccatcaaac
     6541 tctccaaaaa aaacgctatc atttgaccag gtaaccaaag caccagaatc tcaaacagtt
     6601 tttgaacgat acattttatg agcacgctca gagccaataa gttctgtcat atctttaacg
     6661 gttccgcttc cgccagcacc accgccagca tgtcaccaag gtgtatagtt cgcaattaca
     6721 ctaagttcag caaaacgagg tagatcttcg tcacactgaa tttctaagtg agcacatgta
     6781 acttggacat gataatcatc tccaagctct tttcttgcaa gctcgacacc atcaagaaca
     6841 gcttttgaag ctgcttcgcc aacacaatga acgtgcaagt ctaaaccttc tttattaagc
     6901 ttttttagaa gatatgcata ttgctcttta tcaagaagcc taccaccaac tgtaccagta
     6961 tctgcatagg gctcaacta
//
LOCUS       DBNCDC010000061         6845 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_5857_length_6845_cov_7.759499, whole genome shotgun sequence.
ACCESSION   DBNCDC010000061 DBNCDC010000000
VERSION     DBNCDC010000061.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6845)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6845
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5857_length_6845_cov_7.759499"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="AC04VQ_05160"
     CDS             <1..834
                     /locus_tag="AC04VQ_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIO9553531.1"
                     /translation="EAILDYSVYDAIQAGFTKVVFIIRHDFESLFKEKVVSKYKDAIK
                     VELVFQDLNDLPDGYSLPEGREKPWGTAHALLTTNDVVHEPFAVINADDFYSRDAFVK
                     ANEFLTSENIGKSAETQALQFAMVGFQLSNTLSDNGSVARGVCTTDENDNLVGVEELL
                     KVFKTPDGAEDRPEGEAPRTLTGNEPVSMNMWCFTPEVFQYFGDNFIVWLDKNIEVPK
                     SEFLIPTEVDKLIKDGIAKVKLLHTDAKWFGVTYKEDKPIVVDSIQKLVDGGLYPEKL
                     F"
     gene            834..1022
                     /locus_tag="AC04VQ_05165"
     CDS             834..1022
                     /locus_tag="AC04VQ_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553532.1"
                     /translation="MNILDYTFDKYCNGECDILDAHCHIKQAQNFKLICFGLLPSGFP
                     IDKDFENEDIKVGLGFHP"
     gene            1050..1442
                     /locus_tag="AC04VQ_05170"
     CDS             1050..1442
                     /locus_tag="AC04VQ_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIO9553533.1"
                     /translation="MDIFKQFLDEAKFVGEIGLDFGEKYIEHKDKQIDVFTKICDMLK
                     NSKGKTISIHAVKSATEVMDIIEKSGIAKDNIIIMHWFSGSSDELKRAIDSGYYFSVG
                     PRMLATNKGHEYIRQIPEERMFMETDLP"
     gene            1544..2542
                     /locus_tag="AC04VQ_05175"
                     /pseudo
     CDS             1544..2542
                     /locus_tag="AC04VQ_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013300.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
     gene            2657..2851
                     /locus_tag="AC04VQ_05180"
     CDS             2657..2851
                     /locus_tag="AC04VQ_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553534.1"
                     /translation="MVRSVLLDGVVHKTKVKINTGKHITNAHYLDNDQEQIIENNTFM
                     AEPYDYGKSYHTRILQFDLD"
     gene            3006..5336
                     /locus_tag="AC04VQ_05185"
     CDS             3006..5336
                     /locus_tag="AC04VQ_05185"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF005497.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HIO9553535.1"
                     /translation="MIAQIVKRDGRIVDFNEEKIADAVEKAFKACGAMQDRKIADDIA
                     HKVTVKIEEIDSDATPTVEGVQDIVEETLMEEGFIQTSKAYILYRAERERARDISSRL
                     IQTLKDITFSKSSDLDLKRENANIDADTAMGTMLKYGSESSKRFYTMCVIDPKFAEAH
                     ENGDIHIHDMDFYTLTTTCCQLQLSKIFKDGFSTGHGVLREPNGITSYSALACIAIQS
                     NQNDQHGGQSISDFDYGLADGVRKTYRKIYRKHLAEGIELLAEIPDAKTIADDIVTNA
                     EEKHKCWASIDMKSDFRKTVLNGLMANHIDDETAQKIIDYTGRQSFDDTDRQTFQAME
                     ALVHNLNTMHSRAGAQTPFSSVNYGMDTSPEGRMVIKNMLLATEDGLGGGETPIFPVQ
                     IFKVKEGVNFNPEDPNYDLYKLAIHCSAKRLFPNFSFIDAPFNADYYKGTPETEVAYM
                     GCRTRVIGNVADPDRQITPGRGNLSFTSINLPRLALRAKGDVELFFDLLDQKLDLCVG
                     QLDERFEIQCQRKVYNAPFLMGQGIWLDSEKLGPLDEQREVLKHGTLSVGFIGLAETL
                     VALTGQHHGQSQASQNLGLEIVGHMRAYLDKISQERKLNYTLLATPAEGLSGRFVKMD
                     RERFGNIEGITDRDYYTNSFHIPVYFDIDAFTKIDLEAPYHALTNAGHISYIELDGDP
                     TQNLDAFESVIRHMKEAGVGYGSVNHPVDRDPVCGFNGIIGDECPKCGRHEEEGKEFE
                     RIRRITGYLVGTMDRWNDAKTSEEADRVKHGIPKQK"
     gene            5337..5888
                     /locus_tag="AC04VQ_05190"
     CDS             5337..5888
                     /locus_tag="AC04VQ_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015539535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S single cluster domain-containing protein"
                     /protein_id="HIO9553536.1"
                     /translation="MANLEAKINLYGTAPGSIVDGPGIRFGVFVQGCTHHCPKCHNPK
                     SQPHKGGVPTSVADIIKEFEEADSCAGVTLSGGEPFEQAESLAELACALKEKGINVWC
                     YSGYLFEDLIAIANGEDAAGASDYCNKNYAQGVNKLLHNLDVLVDGEFQNDLKSYDAL
                     YRGSKNQRLIDVPKSLENGKVIE"
     gene            6068..6721
                     /locus_tag="AC04VQ_05195"
     CDS             6068..6721
                     /locus_tag="AC04VQ_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIO9553537.1"
                     /translation="MTRDSVPIPSMSIYVNLGRIAVWAIGLTILCSTCFGFDVTGFVA
                     ALGIGGIAISLGFKDTISNLISGVQVSSLKIMVPGDHVSIGNHTGIVIDTTWRHTTIQ
                     DMYGETIIVPNAIINSTAVIKKAPYSTVRVHIQLHSKSAALTAVSDRIIQEVRMAVQN
                     YGTLTEDVKIKFKSIVDGGAKGTVIVKFAEDLDAATTIDIKDVIIKVIAPYVEAAKQ"
BASE COUNT         2054 a         1168 c         1469 g         2154 t
ORIGIN      
        1 gaagcaattc tagactattc tgtttacgat gcaattcagg ctggatttac aaaagttgtt
       61 tttattattc gccatgattt tgaaagtttg tttaaagaaa aagttgtatc taaatataag
      121 gacgctatta aagttgagct tgtttttcaa gatttaaatg atttgccgga tggctattct
      181 ttgccagagg gaagggagaa gccttgggga acggctcacg cattgcttac aacaaatgat
      241 gttgtgcatg aaccttttgc tgttattaat gctgatgatt tttattcgcg tgatgctttt
      301 gtcaaggcaa acgaattttt gacttctgaa aatattggca agagtgcaga gactcaagca
      361 cttcaatttg cgatggttgg ttttcaactt tcaaacactt tgtcagataa tggttcagtt
      421 gcaagaggtg tttgcacaac tgatgaaaat gacaatcttg ttggcgtcga agaacttcta
      481 aaagttttta aaactcccga tggtgccgaa gataggcccg aaggcgaagc gcctcgtact
      541 ttaactggaa acgaacctgt ttcaatgaat atgtggtgtt ttacgccaga ggttttccaa
      601 tacttcggag ataattttat tgtttggttg gataaaaaca tcgaagttcc aaagtctgaa
      661 tttttgattc caactgaagt tgataaatta atcaaagatg gaattgcaaa agttaagctt
      721 ttgcatacag acgctaaatg gtttggcgtt acatacaaag aagacaagcc aattgttgtt
      781 gactctattc aaaaattggt tgacggcggt ttatatccag agaagttgtt ctaatgaata
      841 ttttagatta tacatttgat aaatattgta atggcgaatg cgatattctt gatgcgcatt
      901 gtcatattaa acaagcgcaa aattttaaac taatttgttt tggtcttttg ccatctggtt
      961 ttccaattga taaagatttt gaaaacgaag acattaaagt tggtcttggt tttcaccctt
     1021 gagcctgcgc aaacgcagaa gatattgaat tggatatctt caagcaattt ttagatgaag
     1081 cgaagtttgt tggtgaaatt ggcctggact tcggagagaa atatattgaa cataaagata
     1141 agcaaataga tgtgttcacg aaaatttgcg atatgctaaa aaattcaaaa ggcaaaacaa
     1201 tttcgattca tgcggtgaaa tcggccactg aagttatgga cattattgaa aaatctggaa
     1261 tcgctaaaga caacattatt attatgcact ggtttagtgg aagcagtgat gaacttaagc
     1321 gcgcaattga ttctggatat tattttagtg tagggccacg tatgcttgcc actaataaag
     1381 ggcatgaata tattcgtcaa attccagagg aacgaatgtt tatggagact gatcttccgt
     1441 gagacggaca agagtttaat tcggaggacc actataattt gttaaacgat tttgcgatgc
     1501 agcttcgtga aaccaaatct tactaatata gaaaggaatt attatgaaga ctcaattaaa
     1561 aaagatttta acattatgcc ttggacttgt gttgatttgc ggattggccg gatgtgcttc
     1621 agttagtaag ccggatgcag aaaaagtaga tccaaaatat caatatgtta aggactttga
     1681 gaaacttgga tttggtatgt ttgttcattt tggtctctat agtcaagttg gaaaaggtga
     1741 gtggtattta tatgagaatc ctgaagctaa catgacagat tacgaaaact tgacgaagac
     1801 ttttgatgtt gatcctaact gggctaatga cattgtttct actgcgaagg ctgctggagc
     1861 taaatacgtt actatcacaa caaggcatca tgatggcttt tctctttatg atactaatgg
     1921 gctaaacact tatgatgcgc ctcattcaaa gtgccaccgt gatttaatta aggaatttgt
     1981 tgatacttgt aatgcaaatg gtattaagcc gtttttctat cacacacttc ttgattggca
     2041 cgaagcaaat tacgaagcgg acttcgacac ctatcttgat tacttatata atagtgtaga
     2101 attgctttgc aaaaattatg gcgagattgg tggaatttga tttgatggtt cttgggataa
     2161 gccagatgca gattgaaagt atgatcgcct ttattccatg ataaagtcct atcaaccaaa
     2221 tgctatgatt attaataata ctggtttgga aaagcaaggt gttgtttcgc atcctttaat
     2281 tgatagtgtt acttatgagc gtggaaagcc cgctcgtgta aataatgctg atcgtcctat
     2341 tgctggtgag atgtgtgaga gcttaaccga ccattgggga tacaccgaag aagaccttac
     2401 ctttaagtct gtttctgatc taattgatat tcttactgaa tgtagagcaa ataattgtaa
     2461 tcttttgtta aatgttggtc ctcttgcaaa tggaactatt tcaatatatg aaaaatataa
     2521 aatggaattt ttgggtaagt gaattgcgga caacaagaac tttatctaca acattaaaaa
     2581 gacaaacatt agtgccgaaa atgccgtaat tgttaaggat gataaccatt attatgcaat
     2641 tattggtgat actcctatgg ttcgttctgt tttattagac ggtgttgttc ataaaacaaa
     2701 agtaaaaatt aatacgggaa aacatattac taatgctcat tatcttgaca acgatcaaga
     2761 acagataatt gaaaacaata catttatggc tgagccgtat gattatggta agagttatca
     2821 tactcgtatc cttcaatttg atttagacta aaatcttaaa aaaaatagaa attctcggct
     2881 aattttgcaa taaatatagt atattaggta tacttttcag ctataaaaac cacaatatgt
     2941 tgtggttttt gcgaaaagcc aggtagaatc aataacagtt gattaaatga aattaggagg
     3001 acaaaatgat tgctcaaatt gttaagaggg atggacgcat tgttgatttt aacgaagaaa
     3061 aaattgccga tgctgtcgag aaggctttta aagcttgcgg tgcaatgcaa gaccgaaaga
     3121 ttgcggatga tattgcacac aaagtaactg tcaaaattga agaaatcgat agcgatgcaa
     3181 caccaactgt tgaaggcgtt caagacatcg ttgaagaaac tttgatggag gaaggtttca
     3241 tccaaacatc aaaagcttac attctttatc gtgctgaacg tgaacgtgct cgtgatatca
     3301 gttctcgatt aattcaaaca ttaaaagata ttactttttc gaagtcttcg gacctcgatt
     3361 tgaagcgtga aaatgcaaac atcgatgctg atactgctat gggaacaatg cttaagtatg
     3421 gtagcgagtc ttcgaaacgt ttctatacta tgtgtgttat tgatccaaaa tttgccgaag
     3481 ctcatgagaa cggtgacatc cacattcatg atatggattt ttatactcta acaacaactt
     3541 gttgtcagct tcagcttagc aaaattttca aggatggttt cagcacgggt catggtgtcc
     3601 ttcgtgagcc taatggaatt acatcttatt ctgctcttgc atgtattgca attcagagca
     3661 atcaaaatga tcaacacggt ggtcagtcaa tttctgattt cgattacggc ttagctgatg
     3721 gtgttcgtaa aacttataga aaaatctacc gtaaacattt ggcagaaggc atcgaacttc
     3781 ttgctgaaat tccagatgca aaaacaattg cagatgacat tgtaactaac gccgaagaaa
     3841 agcataaatg ttgggcatct attgatatga agtctgattt tagaaaaact gttttgaatg
     3901 gattgatggc taatcatatt gatgatgaaa ctgctcaaaa gattatcgat tacactgggc
     3961 gccagagctt cgatgatact gataggcaaa cattccaggc aatggaagcg cttgtacata
     4021 atttaaatac gatgcacagt cgtgctggtg cgcaaactcc ttttagttct gttaactatg
     4081 gtatggatac ttctcctgaa ggccgtatgg ttattaagaa catgcttctt gcaaccgaag
     4141 atggtttggg cggcggcgaa actccaattt tccctgttca aattttcaag gttaaggaag
     4201 gtgttaactt taatcctgaa gaccctaact atgatttgta taagcttgca attcattgtt
     4261 cagcgaagcg acttttccct aacttcagct ttattgatgc tccttttaat gctgactatt
     4321 ataagggcac tccagaaact gaggttgctt acatgggttg ccgtactcgt gtaattggta
     4381 atgttgctga tccagaccgt caaattacac ctggtcgtgg taacttgagc tttacgtcaa
     4441 ttaacttgcc acgtttggct cttcgtgcga agggcgatgt tgagctcttc ttcgatttgc
     4501 ttgatcaaaa acttgatctt tgtgttggtc agttagatga gcgctttgaa attcaatgtc
     4561 agcgcaaagt ttataatgca cctttcttga tgggtcaggg tatttggctt gattctgaga
     4621 agttgggtcc gctcgatgaa caacgcgaag ttctaaagca cggaacactt tctgttggat
     4681 ttattggttt ggctgaaact ttagttgcct tgactggtca gcatcatggt caaagccagg
     4741 cgtcacagaa cctcggactt gaaattgttg gtcatatgcg cgcttatctt gacaagataa
     4801 gtcaggagcg caagcttaac tacacgcttt tggcaactcc tgccgaaggc ttgtctggcc
     4861 gcttcgttaa gatggatcgt gagcgcttcg gaaacatcga aggcattacc gatcgtgatt
     4921 actacaccaa cagttttcac attccagtat attttgatat tgatgctttt actaagattg
     4981 acttggaggc tccttatcat gcacttacaa atgctggcca catctcttat attgagcttg
     5041 atggtgatcc aactcaaaac ttggatgcgt ttgaaagtgt aattcgtcac atgaaggaag
     5101 ctggcgttgg ttatggttct gtaaatcatc cagttgaccg tgatcctgtt tgtggattca
     5161 atggaattat tggcgatgaa tgtccaaagt gtggtcgtca cgaagaagag ggcaaggaat
     5221 ttgagagaat tcgtcgtatt acaggttatt tggtaggaac tatggacaga tggaacgatg
     5281 cgaagacttc cgaagaagct gaccgcgtta aacacggaat tcctaagcag aagtaattgg
     5341 caaatttaga agctaaaata aatctttatg gtacagcacc agggtcaatc gttgatggcc
     5401 ctggtattcg ttttggcgtt tttgttcaag gttgcactca tcattgtcca aaatgtcaca
     5461 acccaaaaag tcaaccgcac aaaggcggag ttccaacttc tgtagccgat atcattaagg
     5521 aattcgaaga ggcagacagc tgtgccggtg tgactctaag tggaggtgag ccttttgagc
     5581 aagccgaaag tctagccgaa cttgcatgtg cgcttaaaga aaagggaatt aatgtttggt
     5641 gctattcagg ctaccttttt gaagatttaa tcgcgatagc aaatggcgaa gatgcggcgg
     5701 gagcttcgga ttattgcaac aaaaattatg ctcaaggcgt taacaaactc ctacataatt
     5761 tagatgtatt ggttgatggg gaatttcaaa atgatttgaa gtcttacgat gcgctttatc
     5821 gcgggtcgaa gaaccagcgc ttaattgacg ttccaaaaag cttggaaaat ggtaaagtta
     5881 tagagtgaac gcaggaattt aatgtaccag aaaggccaac atcatggtaa atccgtttga
     5941 tttattttta aattgagtta attcgctaac ttctaatatt tgagttgctg ttgttattac
     6001 tgctatattt atcatagcgc ttactttctt ttttgctgca gttgttgcgg taatcatcaa
     6061 gaggattttg acccgcgact ctgttccaat tccttcgatg agcatttatg tcaaccttgg
     6121 gcgcatcgca gtttgggcaa ttggacttac aattttgtgc tccacttgct tcggcttcga
     6181 tgtgactggt tttgttgccg ctcttggtat tggtggtatc gcaatctctc ttggttttaa
     6241 ggatacaatt tctaacctta ttagtggtgt tcaagtttca tctctaaaaa ttatggttcc
     6301 gggagaccat gttagcattg gaaaccatac cggcatcgta attgatacaa cttggcgaca
     6361 cactactatt caagatatgt atggcgagac cattatcgtg ccaaacgcaa ttattaattc
     6421 aaccgccgtt attaagaaag cgccttatag taccgttcgt gttcatattc aacttcattc
     6481 taaaagtgct gctctaactg ctgtttctga ccgcattatt caagaggttc gcatggctgt
     6541 tcaaaattat ggcaccctga ccgaagatgt taaaatcaaa tttaagagca ttgttgatgg
     6601 tggtgcaaag ggaaccgtta ttgtaaaatt cgccgaagat cttgacgctg caacaacaat
     6661 cgacattaaa gatgtcatca tcaaagtcat cgctccatac gtcgaagccg caaaacaata
     6721 attcactcga tttccccggg gcccacactc gatttactcg tggcatttac tcgattttga
     6781 taatttacat tagaaaaccc cattttcttt cgaggaccca gtttttaaac taggtgggtg
     6841 tccgc
//
LOCUS       DBNCDC010000062         6797 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_5943_length_6797_cov_5.355829, whole genome shotgun sequence.
ACCESSION   DBNCDC010000062 DBNCDC010000000
VERSION     DBNCDC010000062.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6797)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6797
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5943_length_6797_cov_5.355829"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            35..580
                     /locus_tag="AC04VQ_05200"
                     /pseudo
     CDS             35..580
                     /locus_tag="AC04VQ_05200"
                     /inference="COORDINATES: protein motif:HMM:NF037202.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
     gene            595..780
                     /locus_tag="AC04VQ_05205"
     CDS             595..780
                     /locus_tag="AC04VQ_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553538.1"
                     /translation="MNTKGEEKNKKLKTSINRDLSYEEIGNLIKDDPIGRNEELKEVI
                     KFIDSIEGNASIFLDAD"
     gene            892..1062
                     /locus_tag="AC04VQ_05210"
     CDS             892..1062
                     /locus_tag="AC04VQ_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553539.1"
                     /translation="MSEFSIESEQNYLPVYYSAWANDCYDDPLATIVSEIAIKSTKKF
                     NEGLDVDISGIV"
     gene            1087..1977
                     /locus_tag="AC04VQ_05215"
     CDS             1087..1977
                     /locus_tag="AC04VQ_05215"
                     /inference="COORDINATES: protein motif:HMM:NF019313.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HIO9553540.1"
                     /translation="MLGLPAKITDLKNNINKNDFFSSFKKKKELSEKVQKYINNVSKT
                     TNSKILLIIDELDRCKPTFALELLESVKTLFNNENIATLYSVNTSELSKTVSTFYGQG
                     LDGNRYLQRFYDFTFPLKYVPPTIYVKQFPIGERSSHFISAVSQDFANFYKLALRDQN
                     LFYSSMGELMDIKTPNFADETSFGLIKCVAVPLIAGIKFYKFEDYYKIVKNNDYEIFK
                     SYLVNCEQAINIIVSKIFARTNLDKVNDDIREQFIDKFCKEAIGLLFGTLSYDSIDAS
                     KDDVLFGLNCDCKKIFNKYL"
     gene            complement(2011..2427)
                     /locus_tag="AC04VQ_05220"
     CDS             complement(2011..2427)
                     /locus_tag="AC04VQ_05220"
                     /inference="COORDINATES: protein motif:HMM:NF016362.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HIO9553541.1"
                     /translation="MSPKKFEQFSRRLISRMGVEFTEEGTQVSNDGGIDGLGYHRDSN
                     DFRTTRVVIQCKRYNTGDVGSPDIDKFIGAMVKKKADYGIFITNSHFSKAARRAAKEG
                     VPITLINGDELVNLIVEFKLGVTEIKTYELTEYYED"
     gene            complement(2740..2934)
                     /locus_tag="AC04VQ_05225"
     CDS             complement(2740..2934)
                     /locus_tag="AC04VQ_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553542.1"
                     /translation="MSVDYEKLGIIEVEKLLIPAIIKYLQENGGEASRREIKDALIAN
                     DDDIARFSQITKRSKKTGSE"
     gene            complement(3154..3564)
                     /locus_tag="AC04VQ_05230"
     CDS             complement(3154..3564)
                     /locus_tag="AC04VQ_05230"
                     /inference="COORDINATES: protein motif:HMM:NF015444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HIO9553543.1"
                     /translation="MEYSINNNDVLLRLDPGDEIIDCILKVAREANITNAEVSGIGAT
                     DNFEIGVFNLETKQYDLHQFTGNHEITNLCGSITTMNAEAYQHLHITCANSQGQCVGG
                     HLLKAKISLTCEIYIRKAEGTITRKKNESLGINQ"
     gene            3645..4649
                     /locus_tag="AC04VQ_05235"
     CDS             3645..4649
                     /locus_tag="AC04VQ_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HIO9553544.1"
                     /translation="MKDIEKIEPTQIVLYRAGDQDVAVEVYLKDETFWLTQKAMAKLF
                     DCSIDNVSLHLKNIFNDGELDRKATVEDFSIVQNEGNRQVERKMTFYNLEAIISVGYR
                     VNSKKATKFRQWATKVLKEYTIKGFVLDDERLKQGERVFGADYFKELLEKVRSIRASE
                     RRIWQQITDIFAECSINYDKNSEITKEFYSMVQNKFHYAITGKTAPEIIKTSADKSKT
                     NMGLTTWKNSPDGRILKSDVTIAKNYLSMTEIKRLERSVSGYFDYIEDLIERGNTFTM
                     EEFAASVDEFLSFRKYDILKDKGSVSKKNADNFAKAEYDEFNKVRKFISDFDESIDNS
                     "
     gene            4659..5435
                     /gene="proC"
                     /locus_tag="AC04VQ_05240"
     CDS             4659..5435
                     /gene="proC"
                     /locus_tag="AC04VQ_05240"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIO9553545.1"
                     /translation="MDKMIEKKIGFIGCGHMASAIIEGLVESGACSVENITASAAHEK
                     NVFGVSCGTDNVAVAAQSEILIIAVRPADFEKVALEIKDAVCDDAIVLSVAAFITLND
                     MRELFDRDLKFVRCMPNGPVSVGQGMSAVCGDVNDDVLAIFRACGKCEIVDEALLDAV
                     IAVSGSSPVYVYMLIKTMAEKAVELGMSEEFALEFAAQATLGSAQMVQSLDISPDELI
                     EQICTPNGTTIEAVEALRDHGYEEAVRDAMQAAFDKSKGA"
     gene            5439..6587
                     /locus_tag="AC04VQ_05245"
     CDS             5439..6587
                     /locus_tag="AC04VQ_05245"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIO9553546.1"
                     /translation="MGKSKVKNSGRVVAVDVLRGLTICLMILVDDNVGEEFEFLHHPV
                     WTGLSLADFVFPCFITLMGVSMFFSLGKFNFEPNGKSIFRIIRRFVLLFLAGWIISRI
                     CVGIDSVFDGGGFFAAALDLSGLRIMGVLQRIAVCYLFAGLIVLFVHNRKALIGIIVA
                     LLVGYSIILGFGNGYDLSDSNVLIVFDKAVLTPEHMYNKMTTYDGSRMLFDPEGLLSS
                     IPCIAQTLMGFLIGGLLKDKDKDGKTKVLYMFIIGFALLVAGYLISLFIPAFKKAWTM
                     PFVLIMVGVDVGFLALLTWIIDVKKKNKWCKFFQVYGSNAIVSFAAMDILWVLLDDLG
                     LTIIPYNLLLPICFGCEGVASLITTILFILLLYVLLYFLDKKKIYIRL"
     gene            complement(6589..>6797)
                     /locus_tag="AC04VQ_05250"
     CDS             complement(6589..>6797)
                     /locus_tag="AC04VQ_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553547.1"
                     /translation="KNKNFGKFIGAWCFAITAAACLMKMFSAPDTFQLIMNCGMPFIL
                     CGIGLLMPLFARRSKNKFNKRAGK"
BASE COUNT         2003 a         1020 c         1443 g         2331 t
ORIGIN      
        1 caaagatgat attttttatt atatttatgg aatattgcac tcgccagaat ataaaaaaag
       61 atatgagtta aatttacaaa aagaaatacc aagaattcca ttagctgagg agtttgttgt
      121 gttttcaaaa gctggacgca aattggcaga tttacatttg aattatgaaa atgttgagcc
      181 ttgagcagcc atttcagaag aaggagacag taaaaatcct ggaaaaacta ttaaaatgcg
      241 ttttgggaag tgttctgtat caaaagacaa tccaaaaggt attgacatgt caacaattca
      301 tgtttctgaa aacttaacgc taaaaaatat tcctttgcag gcttatgact atattgttaa
      361 tggtaagagc gccattggtt ggattataga taggtatcaa atacggacag ataaagcttc
      421 aggcattgtc aacgacccaa acgaatacag tgacaatcct cgttatatcg tagacctact
      481 taaaagtata gtcacggttt caatccgcac aaatgaaatt atttcaagcc ttccgccact
      541 aaaagaactt ccgcaacctg cagactggcc ttttgaatga aaggcgcaag aataatgaat
      601 acaaaaggag aagaaaaaaa taaaaaacta aagacatcaa ttaatagaga tttgtcgtac
      661 gaagaaatag ggaatcttat aaaagacgac ccaattggaa gaaacgaaga acttaaagaa
      721 gttattaaat ttatagatag tattgaggga aatgcttcaa tatttttaga tgctgactga
      781 ggttctggaa aaactgtatt tgttaaacaa ttggaaatga ttttatgaca attaaacagc
      841 gaaaaagatg atattgatct aaaaaataaa gatgagatta ttcagaaaat aatgagtgaa
      901 ttttctattg agtcagaaca aaattacttg ccggtttact atagtgcttg ggcaaatgat
      961 tgttatgacg atcccttagc tactatagtt tcggaaattg caataaaatc aacaaaaaaa
     1021 tttaatgaag gattagatgt tgatatatct ggcattgtat gaacgtctct agatgccgtg
     1081 atctcattgc tcggcctacc tgctaaaatt actgacctta aaaacaacat caataaaaat
     1141 gactttttct cttcatttaa aaagaaaaaa gaattaagcg aaaaggtcca aaaatatata
     1201 aataatgtat caaaaacaac caattcaaaa atattattga taattgatga gttagataga
     1261 tgtaaaccga cctttgcttt agaactgctt gagtctgtta aaacgttatt taataatgag
     1321 aatattgcaa ctctttactc tgtaaatact tcagagttgt caaaaaccgt ttctactttt
     1381 tatggacaag gtttagatgg caacagatac cttcaaagat tttatgattt tacatttcct
     1441 cttaaatatg taccacccac tatatatgtt aagcaatttc caataggtga aagaagttca
     1501 cattttattt ctgcagtttc gcaagatttt gcaaattttt ataaactagc attgcgcgac
     1561 caaaaccttt tttattcaag catgggagaa ctaatggata ttaaaacacc taattttgct
     1621 gatgaaactt cttttggttt aattaaatgt gttgctgtac cactcattgc gggaataaaa
     1681 ttttataaat ttgaagatta ttataaaata gttaaaaata atgactatga aatatttaaa
     1741 agttatttag taaattgtga acaagctatc aatattatcg tctctaaaat ttttgccaga
     1801 accaacttag ataaagtgaa tgatgatatt agggagcaat ttatcgataa attttgtaaa
     1861 gaggcaatag gcttgttgtt cggtactttg agctatgaca gtattgacgc atcaaaagat
     1921 gatgttttat tcgggcttaa ttgcgattgt aaaaaaatat ttaataaata tctataaata
     1981 gcatattaaa ttaaaattat atttttgagc ttaatcttcg taatattctg taagctcgta
     2041 tgtctttatt tctgtgaccc caagcttaaa ttcaacgata agatttacta actcatctcc
     2101 gtttattaat gttataggaa caccttcctt ggcagctctt ctggccgctt tggagaagtg
     2161 gctattagta atgaatatgc cataatctgc ttttttcttt accatagctc ctataaattt
     2221 atcaatgtct ggagacccta catcgccagt gttatatctt ttgcattgaa ttacgactct
     2281 agttgtgcgg aaatcgttag agtctctgtg ataaccgagc ccatcaattc caccatcgtt
     2341 tgaaacttga gttccttctt ctgtaaactc tacacccatt cttgaaatta gccttcttga
     2401 aaactgttca aacttctttg ggctcatttt tgatattgct tcgagtagct tttgtttaaa
     2461 ttctgcaaag tatttgtctc tcagattttc ttcagttgaa agttcttctt ctttggtagg
     2521 agaggaaggg tcgtttttgt gtttttcttt attttcttca gaacgttttt gtcaatatgg
     2581 atttgatagg agatacactt ctttttcaat gtcaaaattc tttaaattta tttcagagcc
     2641 tttatctgta agagttatat ttcctctgcg cggggcatca acaattcctg caattgcaag
     2701 atttttaatg gaaaaatttg ctctatattc aaacggagct cattcacttc cggttttttt
     2761 ggagcgtttg gttatctgag aaaaacgtgc aatgtcatca tcgttcgcaa ttaatgcatc
     2821 tttgatttct cttcttgaag cttcgcctcc gttttcttgt aagtatttaa taattgccgg
     2881 aattagtagt ttttcaactt ctattattcc tagtttttca tagtcaacac tcatttttag
     2941 ctccttttat aacttaaaga ttattttatc acattgttgg agatagtaag attacgtctc
     3001 ataagtgaga gattttgggt atggagtttc gaaacccctg cagcgcctat ttatagcggg
     3061 cgaacgcacg agggcatacc gcgaagccgt tgagaaaccc atacccaaaa tctctcttgg
     3121 gggactgtgg cttcgggggt taggttagaa ttgtcattgg ttgatgccga gagactcgtt
     3181 tttcttgcgg gtgatggtgc cttcggcttt gcggatataa atctcacagg taaggctaat
     3241 ttttgctttg agtaggtgac ctccaacgca ttgtccttgc gaattggcac aggtaatgtg
     3301 aaggtgttgg taggcttcgg cgttcatggt tgtaatggaa ccgcaaaggt ttgtgatttc
     3361 gtgattgcca gtgaattggt gtaggtcgta ttgtttggtt tctaggttga agacgccaat
     3421 ttcaaagttg tcggtggctc caatgccgct aacttcggca tttgtaatgt tagcttcgcg
     3481 ggcaactttt aaaatgcaat cgatgatttc atcgccggga tcaagtctta gtagtacatc
     3541 gttgttgttt attgagtatt ccatggtatc tcctttttgc gttattttat caaaattatt
     3601 taaattaaca taaaagaaca aatgttctgt gttataatga gcgcatgaaa gatattgaga
     3661 aaatagagcc aactcaaatt gttctttaca gagcagggga ccaggatgtt gcagttgagg
     3721 tttatttaaa ggatgaaacc ttttggctta cgcaaaaagc aatggcaaag ctttttgact
     3781 gcagcataga caatgtttct ctgcatttaa aaaacatttt caatgatggt gaattagaca
     3841 gaaaagcaac tgtcgaggat ttctcgatag ttcaaaatga agggaatcgc caggttgaac
     3901 gtaaaatgac attttataat ctagaagcaa taatttctgt tggttatcgt gttaattcta
     3961 agaaggctac aaaattcagg cagtgggcta caaaggtttt gaaagagtat acaattaagg
     4021 gatttgtttt agatgatgag aggcttaaac aaggcgagcg tgtcttcggt gctgattatt
     4081 ttaaagagct acttgagaaa gttcgctcga tacgcgcgag tgagcgtagg atttggcagc
     4141 agattactga tattttcgct gagtgcagta ttaattatga taaaaattct gagattacaa
     4201 aagagtttta ttccatggtt caaaacaaat ttcattatgc aattactggt aaaactgctc
     4261 cggagattat taagacttcg gctgataaaa gcaagacaaa tatgggtttg accacatgga
     4321 aaaattctcc tgatgggcgg attttaaaat ctgatgttac tatagccaaa aattatttga
     4381 gcatgacaga aattaaaaga ctcgagagat ctgtgtctgg ctattttgat tatattgaag
     4441 atttgattga gcgtggtaac accttcacca tggaagaatt tgcagctagt gttgatgagt
     4501 tcttgtcttt tcgaaaatac gatatattga aagacaaagg aagcgtttct aagaaaaatg
     4561 cggataactt cgctaaagcg gagtatgatg aattcaataa ggttagaaaa ttcatttctg
     4621 acttcgatga aagcatagac aatagttaaa ttaaatgtat ggataaaatg attgagaaaa
     4681 agattgggtt tattggttgc gggcatatgg ccagtgcgat tattgaggga ttggttgaaa
     4741 gtggtgcctg cagcgttgag aatattacgg cgagtgccgc gcatgagaag aatgtttttg
     4801 gtgttagttg tgggactgat aatgttgcgg tggccgcgca aagtgagatt ttgattatag
     4861 ctgtgcgacc ggccgatttt gaaaaggttg ctcttgagat taaagatgcc gtttgcgatg
     4921 atgctattgt gctttctgtt gcggcgttta ttaccttaaa cgatatgcgt gagctctttg
     4981 atagagattt gaaatttgtt cgctgcatgc caaatggtcc agtgagtgta ggacagggca
     5041 tgagtgctgt ttgcggagat gtcaatgatg atgttttggc gatctttcga gcttgtggca
     5101 aatgtgagat tgttgatgaa gccttgcttg atgctgtgat tgcggttagt gggtcttcgc
     5161 cggtttatgt ttatatgcta attaagacca tggctgaaaa ggcagttgaa cttggaatga
     5221 gtgaggagtt tgctttggag tttgcggcac aggcgacact tggatctgct caaatggtcc
     5281 agagtttgga catttcgcca gatgaattaa ttgagcaaat ttgcactcct aatggaacta
     5341 caattgaggc ggttgaggcg cttcgtgacc atggctacga ggaggcagtt agggatgcca
     5401 tgcaggctgc cttcgataaa tctaaaggag cttagtttat ggggaaaagc aaagttaaaa
     5461 atagtgggcg agttgttgcg gttgatgttt tgcgcggctt aactatttgc ttgatgattc
     5521 ttgttgatga taatgtgggt gaggagtttg agtttttgca tcatcctgtt tggactggac
     5581 tttcgcttgc tgattttgtt tttccttgtt ttattacgct tatgggtgta tctatgtttt
     5641 tttctttggg taaatttaat tttgaaccaa atggaaaatc gatttttaga attattcggc
     5701 gttttgtttt gttgtttttg gctggttgga ttatttcgcg aatttgtgtt ggcatagatt
     5761 cggtgtttga cggtggggga ttctttgctg cggcgcttga tttatcgggt cttagaataa
     5821 tgggtgtttt gcagcgtatt gctgtttgtt atttatttgc tggcttgatt gttttgtttg
     5881 ttcataatag gaaagcgctt attggaatta ttgttgcgtt gcttgttggc tattcgatta
     5941 ttttaggatt tggcaatggc tatgatttaa gtgattctaa tgtgcttatt gtttttgata
     6001 aggctgtgct tactcctgaa catatgtata acaaaatgac tacttatgat ggcagtagga
     6061 tgttgtttga tcctgagggt ttgctttcaa gtattccttg cattgctcaa actttgatgg
     6121 gctttttaat tggtggatta cttaaagaca aagataagga tggcaagaca aaagttttgt
     6181 atatgtttat tattggtttt gctcttcttg ttgcgggtta tttgatttcg ctatttattc
     6241 ctgcctttaa aaaggcttgg acaatgccat ttgtgctaat tatggttggt gttgatgttg
     6301 gtttcttggc cttgcttacg tggataattg acgttaaaaa gaaaaacaaa tggtgtaagt
     6361 tttttcaagt gtatggttca aacgcgatcg tcagcttcgc cgctatggat attctttggg
     6421 tgcttttaga cgaccttggt cttacgataa ttccgtataa tcttttactt ccaatttgtt
     6481 ttggatgtga gggagtggca tcgcttataa caacaatact ttttatattg ctgctctacg
     6541 tgcttctcta cttccttgac aagaagaaaa tctacatccg cctataattt atttgccggc
     6601 gcgcttgttg aatttgtttt tgcttcggcg agcgaatagt ggcattaata ggccaattcc
     6661 gcaaaggata aatggcattc cgcaattcat gattaattgg aatgtatccg gtgcgctaaa
     6721 catcttcatt aagcatgctg ctgctgtgat agcaaagcac caagcaccaa taaatttacc
     6781 aaagttcttg tttttga
//
LOCUS       DBNCDC010000063         6681 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_6138_length_6681_cov_6.079686, whole genome shotgun sequence.
ACCESSION   DBNCDC010000063 DBNCDC010000000
VERSION     DBNCDC010000063.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6681)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6681
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6138_length_6681_cov_6.079686"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            289..1656
                     /locus_tag="AC04VQ_05255"
     CDS             289..1656
                     /locus_tag="AC04VQ_05255"
                     /inference="COORDINATES: protein motif:HMM:NF021028.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIO9553548.1"
                     /translation="MVETFKLLLHFVAASTPVPPGPGPEPTPTPGGGGTSVASQTGDI
                     NLLLFFAVLFVAIAAVLMLFLAARAKAQGTNMKSVAVNYAFKFATNARLAVIGALVVA
                     AIALLCGITLANAFATSNEDPSNTVNVYVNEETGVITSIDQGTYTNETGHNIAVSDIA
                     TSIAEEAQSVTGLENATLTIMNGTQIFYQGKIGTSYTPTTTTYVADGQTLTFTFEFKD
                     LAADTALQLIDKDAVGLSFNEKGYHTITYDGNGNTGGTAPDPFIKIDDTTAKISGNTG
                     SLVKGEATFNGWNTNAEGTGTAYVEGQNYTDNEDLKLFAVYKEPTTDVTLTYKIIDEY
                     QDMSHGSVALTSGGSAAWQVTESFNPDSATPVGAKATPKDTEENDVFGNWEFSHWSIV
                     KDGEEVCVSESAQFVPTLETLGLTTWADTTITAHFGKAVALFDLNAETLTFKYTEDED
                     VLAES"
     gene            1816..2040
                     /locus_tag="AC04VQ_05260"
     CDS             1816..2040
                     /locus_tag="AC04VQ_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553549.1"
                     /translation="MDMWFASSKTLASLYNRSAQVANRKLDKIEGLENIDTSEVVSTV
                     AMFADNCGKMMVQSITQEERFSFSTIDLSS"
     gene            2092..2820
                     /locus_tag="AC04VQ_05265"
     CDS             2092..2820
                     /locus_tag="AC04VQ_05265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553550.1"
                     /translation="MQQVLLPTDQTSVEGSFGTTVENMDDMFLYCGRLTNLSISTNFG
                     AKAKTTNDMFNGCSSLKAIDAPVGNKFLSSCTECSDMFYGCQGLRKVDLSNLNVNNNI
                     DAEDIQGALYGCDELRYVTVGAGTTWTNSDLSLDTMGLQNVDYDENGIWYSTDEGTES
                     PNKAEDIPRAHIDQKTNITYRTLCSKAIYNDAANAKSLTFYYDHKVYSTVEFSLDTAL
                     NTKYMMQDTGGATYGMYDKPIFPE"
     gene            2872..3054
                     /locus_tag="AC04VQ_05270"
     CDS             2872..3054
                     /locus_tag="AC04VQ_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553551.1"
                     /translation="MGEYDYATLIPNLDLKITLTIDAVDVYFDTSFKNCPDLYSMAYF
                     FMADSTGKQTFKNFYN"
     gene            3094..3402
                     /locus_tag="AC04VQ_05275"
     CDS             3094..3402
                     /locus_tag="AC04VQ_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553552.1"
                     /translation="MFGGFSYKSIAQSSYNPYGLISLYSNVPELDKDPYINAYANDTY
                     GTALESIDISGLNIFPEYSNKGDTAPPLNFDFMFANCTKLQNVKFPTIPNNAKDTMER
                     "
     gene            3811..3981
                     /locus_tag="AC04VQ_05280"
     CDS             3811..3981
                     /locus_tag="AC04VQ_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553553.1"
                     /translation="MAQANISSVDTTQGTVSPGTVNFVGTVIVIPLADGNIKVGDTVV
                     KATPADGYEVAY"
     gene            4132..4320
                     /locus_tag="AC04VQ_05285"
     CDS             4132..4320
                     /locus_tag="AC04VQ_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553554.1"
                     /translation="MTMYYDDQTHAGEGRVFDINPNTYLQPSLDAERIPAFHPEVLLS
                     NSENGQSNADSNNIYTGY"
     gene            4483..4806
                     /locus_tag="AC04VQ_05290"
     CDS             4483..4806
                     /locus_tag="AC04VQ_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553555.1"
                     /translation="MADSDNDQTIKTFKSVDSNGNELAPFANLNTTSIQNVEYAFGGL
                     YLNRYFDLKGSAAAASAADLLFDTLLGDPASAVTYNSSYNTYGNSIKELDMSGFNFSK
                     LRSMD"
     gene            4807..5058
                     /locus_tag="AC04VQ_05295"
     CDS             4807..5058
                     /locus_tag="AC04VQ_05295"
                     /inference="COORDINATES: protein motif:HMM:NF015347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HIO9553556.1"
                     /translation="MFANCQGVEKIKFDDNFDTSYLNSMEGVFNNCENCKEILGLAGS
                     KVNTINVDNMDNLFQGCSNLTSLDLSKFDTRNVYKNPEF"
     gene            5332..5625
                     /locus_tag="AC04VQ_05300"
     CDS             5332..5625
                     /locus_tag="AC04VQ_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553557.1"
                     /translation="MQGTLKRTPPDIYKVDIKTGDSSKGQIAYLNKMQEQWQYDYINS
                     IRISTIQDAPAAEADVPADITNEETTVAALYVDDRTYFAVPENGYELQNWTAT"
     gene            5755..5931
                     /locus_tag="AC04VQ_05305"
     CDS             5755..5931
                     /locus_tag="AC04VQ_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553558.1"
                     /translation="MLSEDSNGNGYFSFRYDRGVYAPGTQLEITLGKDKLADTTVIKA
                     YKVSYNHDDYNLPL"
     gene            5959..>6681
                     /locus_tag="AC04VQ_05310"
     CDS             5959..>6681
                     /locus_tag="AC04VQ_05310"
                     /inference="COORDINATES: protein motif:HMM:NF015347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HIO9553559.1"
                     /translation="MTQDPHPYIPGFDEVFSKYSLDNEPLTIVFEDNFKDFNTLESLS
                     CFFMQAPRAGDGYGALVTPFAFDDTHFKGLSNICMDHVWNVKYMFGGHEYFNDINGSK
                     AFETIDLTGLDMSKLNNLDAMFANCLNLKSIVWPEGFDCSKITSMYKTFFNCDALYGN
                     DLLKVLENLDTSNVTNMSYMFAKRHFYNEEQQLQNEAIDIDYIFPSKFSVASVTNMTR
                     MFAGNEFAKSINIKTKTTLFELF"
BASE COUNT         2223 a         1190 c         1255 g         2013 t
ORIGIN      
        1 aatgccgtat cacctaacgc tgaagcctcc aacttgaaaa gccactggat ctatactatg
       61 aaacaattca ttcatacttt atattataaa agaattattt tgaagcgaaa ataaaaatat
      121 ttaacgtagg aaaatttgtt tttaacccct cgctgccccc ggccccaaaa gtttctttag
      181 aaggagggaa aaggggaggt caaacgtcgt ttattttgcc aggtcgattt gacaaaaaaa
      241 aaaaacgatt aaaataggat aattgtcaag aaagaagggg agatcatcat ggttgaaaca
      301 tttaagcttt tactacattt tgttgctgca agcactcctg tcccaccagg tccaggtccg
      361 gaaccaactc caactcctgg cggcggagga acttctgtag catcacaaac aggcgacatt
      421 aatttgcttc tgtttttcgc cgtgcttttt gtcgcgattg cagcagtttt aatgctgttt
      481 ttggcggcgc gagcaaaagc gcaaggcaca aacatgaagt cggtcgcggt aaattatgcg
      541 tttaagttcg caacaaacgc caggctcgcg gttatcggag ctctcgtagt tgcagcaatt
      601 gcgctgttgt gtggaatcac gctggctaac gctttcgcaa cttcgaacga agacccgtcc
      661 aacactgtca acgtttatgt caacgaagaa acgggcgtaa tcacgtcaat tgatcaaggc
      721 acctacacta atgaaacagg gcataacatt gcagtttcgg acatcgcaac ttcaatcgcc
      781 gaagaagccc aaagcgtcac tggccttgaa aacgcgacat tgacaattat gaacggcact
      841 caaattttct atcaaggaaa aattggaact tcttatacgc ctacaactac aacatatgtt
      901 gccgatggtc aaacgctgac attcacattt gaattcaagg acctcgccgc ggatactgca
      961 ttgcagttaa tcgataaaga cgcagttggt ctttcgttca atgagaaagg ctaccataca
     1021 atcacatatg atggaaatgg taatacaggt ggtaccgccc ctgatccatt cattaagatt
     1081 gatgacacaa ctgcaaaaat ttcaggaaac accggcagcc ttgttaaagg cgaagccaca
     1141 ttcaacggct ggaacacaaa cgccgaagga accggcacgg cttacgttga aggtcaaaat
     1201 tacaccgaca acgaagacct caaattgttt gcagtctaca aagaacctac aaccgatgtc
     1261 acccttacat ataaaattat tgatgaatat caagacatga gccatggctc agttgctcta
     1321 acttctggtg gaagtgctgc atggcaagtt acagaatcct ttaaccctga ttctgcaact
     1381 cctgttggtg ccaaagcaac acctaaagac accgaagaaa atgacgtttt tggcaattgg
     1441 gaattctccc attggtcaat tgttaaggac ggcgaagagg tttgcgtaag cgaatctgca
     1501 cagttcgttc caactctcga aacgcttggc ctaacaactt gggctgacac aacaatcact
     1561 gctcactttg gaaaggctgt agcgctcttc gaccttaatg cagaaaccct tacatttaaa
     1621 tacaccgaag acgaagacgt ccttgctgaa agttgagcat tacctgtcta caaagacgct
     1681 catattgaca tagcgaagca agagtttaag cttccttctt ggtttacaag tccagaaact
     1741 ggtggaactt taaaatgcta tccaagaaca gcaattattg aaaaggattt taagcacttc
     1801 aaaggcatta caagcatgga tatgtggttt gcaagttcaa agacacttgc atctttatac
     1861 aataggtctg ctcaagttgc aaatcgaaaa cttgataaaa ttgaaggact tgaaaatatt
     1921 gacacatccg aagttgtttc aactgtagct atgttcgcag acaattgtgg aaaaatgatg
     1981 gtgcagtcca tcactcaaga agaaagattt agttttagca ctattgatct ttcatcatga
     2041 gtaacaccta aactcaagga tacatcttat atgtttgccg attgttgaaa tttgcaacaa
     2101 gttttgttgc caactgatca aacttcggtt gaaggttctt ttggaaccac agttgaaaat
     2161 atggatgata tgttcttgta ttgtggtcgt cttacaaact taagcatttc tactaatttt
     2221 ggtgcaaaag ctaaaaccac aaatgatatg ttcaacggct gcagttcgtt aaaagcaatc
     2281 gatgcaccag ttggaaataa gtttctttct tcttgtacgg aatgttcaga tatgttttat
     2341 ggatgccaag gattgcgcaa agttgatctt agcaacttaa atgtaaacaa caatattgat
     2401 gcagaagata ttcaaggcgc actttatggt tgtgatgaac ttcgttatgt aactgttggt
     2461 gctggaacaa cttggacaaa ttctgacttg tctcttgaca caatgggtct tcaaaatgta
     2521 gattatgacg aaaatggtat atggtattca actgacgaag gtactgaatc tccaaataaa
     2581 gccgaagata tcccgagagc tcatatcgat caaaaaacaa atattactta cagaactctt
     2641 tgctcaaaag ctatttacaa tgatgcagca aacgcaaaaa gcctaacatt ttattatgac
     2701 cataaagttt atagcactgt agaattttct ctcgacactg ccctaaatac aaaatacatg
     2761 atgcaagaca caggtggtgc aacttatgga atgtatgata aaccaatctt tcctgaatga
     2821 tatagttata ctgcaactgc ggatggggca aatgttggca cctatcagat attgggtgaa
     2881 tatgattacg caacattaat ccctaatctt gatttgaaaa ttacacttac aatagatgct
     2941 gtagatgttt attttgatac ttcatttaag aactgtcctg acctttatag tatggcatat
     3001 ttctttatgg ccgactcgac cggcaagcaa acatttaaga acttctataa ttgagagaat
     3061 gtaaacacac aaaatatcta tagctatgct tgcatgtttg gaggcttttc ttataagtca
     3121 attgcgcaaa gctcttataa tccttatggg ttaatttctc tttattctaa cgtacccgaa
     3181 ttggataaag acccttatat taatgcctat gcaaacgaca catatggtac agctttagaa
     3241 agtattgata tttcaggctt aaatattttt cctgaatata gtaataaggg tgataccgca
     3301 cctcctctaa actttgactt tatgtttgca aattgcacga agctacaaaa tgtaaaattc
     3361 ccaactattc ctaataatgc aaaggacacc atggaaagat gaacaaggtt taaccataca
     3421 ttcgaaaatt gtcaatcttt aacaagtgtt cctgatacat taaaagaatc ttactatatc
     3481 tcatatgctc aatatatgtt ttggaattgc actaacctta aaacagcaga tttagaaggt
     3541 tgaagagtta attcatcaac acaatttagt gactatggtt ataatatgtt ttccggatgc
     3601 aataatctta ttactgttaa aactgatgaa aattgatgtt ggaggatgga tcaatgcgga
     3661 cttatcgaaa cttggcctta tggggaagaa gtagataact ataatccttg aatacttaat
     3721 ggcgataata agcatgtata tgaaatacca tttgtcgatt ctacgataac aaacttcggt
     3781 acattttcaa gaggtgaaaa tactaaaggt atggcacaag ctaatatttc aagtgtcgat
     3841 acaacacaag gtaccgtaag tcctgggaca gttaatttcg ttggaactgt tattgttatt
     3901 ccactcgcag atggaaatat caaagttgga gacactgttg taaaggcaac tcctgcggat
     3961 ggttacgaag ttgcttattg aacatgcaat ggtaagaaaa ttattgacgt tgatattaat
     4021 agtaaattac ctcaattaat tgcaaacgac gattcaccaa aggatttgca agttcacttt
     4081 agaaaagcaa attataggcc aaaagcagtt tataatgaaa cctcaaagac aatgaccatg
     4141 tattacgatg atcaaactca cgcaggcgaa ggtagagtat ttgacatcaa tccaaacaca
     4201 tatttacagc cttctttaga cgctgaaaga atccctgcat ttcatcctga agtcttactt
     4261 tcaaattctg aaaatggcca aagcaatgca gattcaaata atatatatac aggctattga
     4321 gattttagac aatttgaaat ttcacttcca agttggtatg aatttaggca agataacgaa
     4381 aatcaacgtt tctatattaa tagtttagta tcgccagaaa cagttgtatt tgattcaaca
     4441 tttgcagatt atgacgaact tcaaagtatt tcaatgtggt ttatggcgga ttccgataat
     4501 gaccaaacaa ttaaaacctt caagagtgtt gatagtaatg gtaatgaact tgctccgttc
     4561 gcaaacttaa atacaacaag cattcaaaat gttgaatacg cttttggtgg gctttattta
     4621 aataggtatt ttgacctcaa gggttcagct gccgctgctt ctgctgcaga cttgttgttc
     4681 gatactttat taggagatcc tgcaagtgct gtaacttaca attcgtcata taacacttac
     4741 ggcaattcaa ttaaagaact tgatatgagt ggctttaatt tcagcaagtt aagatcaatg
     4801 gattgaatgt ttgcaaattg tcaaggagta gaaaaaatta agtttgatga taatttcgat
     4861 acatcttatt taaattccat ggaaggtgtt ttcaataatt gtgaaaattg taaagaaatt
     4921 ttaggccttg caggttcaaa agttaatact ataaatgttg ataatatgga taatttgttc
     4981 caaggctgca gcaatctaac aagtcttgat cttagcaagt ttgatacaag aaatgtttat
     5041 aagaacccag aattttgagt tattatcaaa acatctaatg gaagatcacc tttcactcat
     5101 aataaaatgt tcttagattg tgttcgatta aacgagataa cgataacttc tacttatgat
     5161 tcaatcaatg atgaatgaac aggttgaact ttgtatatgg atatgtgcga tttgccaagt
     5221 aattcaaatc tttatgataa ttgaagagta aatgggcaag gagaatctta ttcgcctcat
     5281 tatattccat taaaaataga cgataatagc ccaacaagcc aagatgattt agtgcaggga
     5341 actttaaagc gtactccacc agatatatat aaagttgata taaagactgg agattcctca
     5401 aagggacaaa ttgcttatct aaataaaatg caagaacaat ggcaatatga ttatattaat
     5461 tctatacgta tttctactat tcaagatgct ccagcagctg aagcggatgt tcctgctgat
     5521 ataactaatg aggaaactac cgttgctgcc ttatatgttg atgatcgtac gtattttgcc
     5581 gttccagaaa atggttatga attgcaaaat tggacagcta cttgagaaga cgggtcaggt
     5641 acgacacata cttgggtttt gaatgatcaa ggatcgcttt acttggacgg cgataataaa
     5701 gaaaacatta ctgtcactgc ttcatttaag gaaaaaggtg ccgaagcttg agccttgctt
     5761 tcggaggatt ccaatggtaa cggctacttc agctttaggt atgacagagg tgtttacgct
     5821 cctggaacac aacttgaaat aactcttggg aaagataaac ttgctgacac aacagtaatt
     5881 aaagcttata aagttagtta taatcatgat gactataatc tcccattatg atatgacggt
     5941 tcgtcaattc aacatttttt gacacaagat ccacatcctt atattccggg ctttgacgag
     6001 gtatttagca aatattcatt agataatgaa cctcttacca ttgtttttga agataacttt
     6061 aaagacttta atacattaga aagcttatcg tgcttcttta tgcaagcacc aagagcaggt
     6121 gacggatatg gagctttggt tacaccgttt gcttttgatg atacgcattt taaaggctta
     6181 tctaacattt gtatggatca tgtttggaat gtaaagtata tgtttggtgg ccatgaatac
     6241 tttaatgata ttaatggttc taaggctttc gaaacaattg atttgactgg tcttgatatg
     6301 agcaaattaa ataatcttga cgctatgttt gcaaattgct tgaacttaaa atcaattgtt
     6361 tggccagaag gcttcgattg ctcaaaaatt acaagcatgt ataagacctt ctttaattgt
     6421 gatgctttat atggcaatga tttgttaaaa gtattagaaa atttagatac ttctaatgtt
     6481 acaaatatgt cttatatgtt tgcaaaaaga catttttaca atgaagaaca acaacttcaa
     6541 aatgaagcaa ttgatattga ttatattttc ccatcaaaat tctcagttgc atccgtaact
     6601 aatatgacca ggatgtttgc tggaaatgaa tttgcaaaat caattaacat caagactaaa
     6661 acaactttat ttgaattgtt t
//
LOCUS       DBNCDC010000064         6546 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_6366_length_6546_cov_5.654907, whole genome shotgun sequence.
ACCESSION   DBNCDC010000064 DBNCDC010000000
VERSION     DBNCDC010000064.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6366_length_6546_cov_5.654907"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            42..329
                     /gene="rpsO"
                     /locus_tag="AC04VQ_05315"
     CDS             42..329
                     /gene="rpsO"
                     /locus_tag="AC04VQ_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009307039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIO9553560.1"
                     /translation="MADKLSITKETSQKLIKDFGKSDKDSGSAEVQVAILSERIKNLT
                     EHLKEHPKDNHSRTGLMKLIGKRRGLLVYIKNKDIDQYRSLIKKLGIRDNV"
     gene            407..2572
                     /locus_tag="AC04VQ_05320"
     CDS             407..2572
                     /locus_tag="AC04VQ_05320"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIO9553561.1"
                     /translation="MEKIVYDFELYGKKYCLQTGELAKQTSGAVLVSQGDTNVLVTSV
                     VSTEEKNYDFFPLTVDFIEKMYAVGRIPGGYLKREAKPSDKATLIARMIDRPIRTGFP
                     DGFKQEVHVVATSLVCDGENPPDTISIMGASAALMVAGAPYEGPSACVRIGRDPETKE
                     FIVNPTYAEDEASDLELTIAGTKDYISMVEAAADEVSEDVMLEAMTFAQEAIGKFCDE
                     QQKFIDMVKPEKMEFSIHEPDHKVVELVTAHASDMDAAVNDADIDSRKEKIHELKESI
                     TAEFSEEEQEEWAADIAAELKTLEKHSMRDMVIKTKHRIDGRELTEIRPLHIVADYLP
                     RVHGSGLFQRGQTQALSICTLGMLTDAQRLDTIDPQTTKRYMHHYNFPPYCTGEVGRM
                     GSPKRREIGHGALAEKALVPVLPSEEEFPYAIRVVSEIMESNGSSSMASTCGSTLALM
                     DAGVPIKRPVSGIAMGLIKEGDDVVILSDIQGLEDFLGDMDFKVCGTEQGITALQMDN
                     KAKGLSVDILSAALTQAKEGRAFILDHMLEAIAVPRSEMKEHTPKIETIKIDKDKIRD
                     VIGKGGEMIRSIQEQTGAEIEILEDGTTRIFAAMKSVGDAAKEMIKNIVKEPEVGEEY
                     NGEVVAIKDFGAFVRLTPGKDGLLHISKMAQGRVRRVEDVLNIGDEVKVKITDIDRGG
                     KISLDRIDKPSVEGGDGVEYDVVDTESHGGYEGRAKRRR"
     gene            2597..4375
                     /locus_tag="AC04VQ_05325"
     CDS             2597..4375
                     /locus_tag="AC04VQ_05325"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIO9553562.1"
                     /translation="MQKENNKKPQKKNKQNRNRTHHYKHVQVNKVRPKLSRGNFDQDQ
                     NKKQAAKRGSVQKDKNAKLRVIPLGGLDAIGDNMTVFECENGMILDDAGMMFPSDAHP
                     GVDLILPDYTYVLEHADKLKAIVITHGHEDHIGCLPYLIQDLGRPVPIFATKLAIGFI
                     KAKFEEFDIKNISFNEIKPGDVINTGCFQISFFQVNHSIPGCVGMFIQTPAGNILHTG
                     DFKLDQTPIDGMTTDFDLISKFAKTGIDLLMSDSTNANVKEFTPSESTVGPELERIIR
                     DAKGKVIVATFASHIHRVQQVCDAAIKCGRKVVVAGRSMVQNVEIARKLGYLHVSDEH
                     IIDAYELDNIPSSDAVILCTGSQGEPLSALARVSTDSHKRITAEKGDTVIISATPVPG
                     NEKAVSAVVNNLAKLGVNVVDKTIAPVHVSGHAASEELKMMISLCNPYGFMPVHGEAV
                     HLLAHAKLAEQCGVDHDKIFLCENGDVIEMSTSGLRRLEPVQSGVVYVDGLSVGDTSQ
                     DVLKERNFLGEGGIAVISAAINYRQKQILGPVYVNLRGVSGGDDPDLQDELRNAVVRA
                     LNKELKKSTDNNHIRKAAKDALLSLL"
     gene            4445..4729
                     /locus_tag="AC04VQ_05330"
     CDS             4445..4729
                     /locus_tag="AC04VQ_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553563.1"
                     /translation="MAQKKQNKETKKTASFGKQKRTNNGKVKQVKHGEEIIDSRAKTD
                     IIAVVLIVVGVALFAVSLVPTDAPVTSFLVNILRFIFGVGVFIFPFALII"
     gene            4751..4927
                     /locus_tag="AC04VQ_05335"
     CDS             4751..4927
                     /locus_tag="AC04VQ_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553564.1"
                     /translation="MERQKISVRVAIGLSLIFVAILTIISLCSTAIAMNRPEQLFEYE
                     TIINYGGIIGSALS"
     gene            4940..>6546
                     /locus_tag="AC04VQ_05340"
     CDS             4940..>6546
                     /locus_tag="AC04VQ_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009307033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIO9553565.1"
                     /translation="MLGNVVSLILMIGVIIAGLIVIGLSITTIVTRINERREDIEREL
                     EERKRARLAAKAAREAEEAGDEQGEETVAIDAAVQKTMAIEDNKGKKPGLKTRIMGFK
                     DQKEKDKEEFDYDPNQNKTRQIGSPAGSESKKKTSETSKDQASTPSAPKTQVIGPKPM
                     EGFVLPSFDILKAPSEGGEVTQSEESLKAKAEKLKETLEEFGIKANVVGWVPGPTVTL
                     FKVDLPAGVRVSRITALNDDIALALAAPGVRVFAPIPGTTYVGIEIPNDDRQTVLLSE
                     VLGKAKKGPLQMAIGKDVEGNAITANLATMPHLLIGGTTGSGKSVAINAMITSILMRA
                     TPSEVKLILIDPKRVEFTPYNGIPHLYVPVVTEPKEAASALTWGVAEMESRLKLFSEH
                     KVRNIEAYNSMVQKMSKRDREAADLSELPYIVIVIDELADLMMAVGKEVEFSISRIAQ
                     LARAAGIHLIVATQRPSTNVVTGLIKANITTRIAFNVASGIDSRVILDTGGAESLIGL
                     GDLLFSKPEFSKPLRVQGCFVTEDEISA"
BASE COUNT         1982 a         1248 c         1534 g         1782 t
ORIGIN      
        1 gggccaagag cgcaggtaac tgcgtaaaaa ggagacatat tatggcagat aagttaagta
       61 ttacaaaaga aacaagccaa aagctaatta aagattttgg taaaagcgac aaagattcag
      121 gaagtgccga agtccaggta gctattctat ctgaacgtat caaaaatctt actgagcacc
      181 taaaagaaca tccaaaagac aatcactcaa gaacaggctt gatgaagctt attggtaaaa
      241 gacgtggtct tttggtttac atcaagaaca aagatatcga tcagtatcgt tctttgatta
      301 agaaacttgg cattcgtgat aacgtgtaat taaagcgggc tttgtcccgc tttttgacaa
      361 ttgaatagtt tgtaaataaa cgaactagaa acgaaacgag gagaaaatgg aaaaaattgt
      421 ttatgacttt gaactctacg gcaagaaata ttgccttcaa actggggaat tagcgaagca
      481 aacatctggt gctgttttag tttcccaagg cgacacaaat gttttggtta caagtgtcgt
      541 ttccaccgaa gaaaaaaatt atgatttctt cccactaaca gttgatttta tcgaaaaaat
      601 gtatgcggta ggtcgcattc ctggaggcta tttaaagcgc gaagctaagc cttcggataa
      661 ggcaactttg attgctcgca tgattgaccg tccaattaga acaggttttc ctgatggctt
      721 taagcaggaa gttcatgttg tagcaacatc tcttgtttgc gatggcgaaa acccaccgga
      781 tacaatttct atcatgggcg cctccgcagc gttgatggta gcaggtgctc catacgaagg
      841 tccaagtgca tgcgttcgca tcggtcgcga cccagaaact aaggaattta ttgttaatcc
      901 aacttatgca gaagacgaag caagcgacct tgagcttacc attgctggta ccaaggacta
      961 catctctatg gtcgaagctg ctgctgatga ggtcagcgaa gacgtgatgc tcgaggctat
     1021 gacatttgct caagaggcta ttggaaaatt ctgcgatgag cagcaaaaat tcatcgatat
     1081 ggtcaaacct gaaaagatgg aatttagcat ccacgagcca gatcataagg ttgttgaact
     1141 cgtaacagcc catgcatcag acatggatgc tgctgtaaac gatgccgaca tcgactctcg
     1201 caaagagaaa attcacgagc ttaaggaaag cattactgct gaatttagcg aagaagagca
     1261 ggaagaatgg gcagctgaca ttgcagctga actcaaaact cttgagaagc attctatgcg
     1321 cgatatggtc attaagacca agcacagaat tgacgggcga gaactaaccg aaattcgtcc
     1381 gcttcatatt gtggctgatt atttgccacg agtccatgga tcaggacttt tccagcgtgg
     1441 tcaaactcag gcgctaagta tttgcactct tggaatgctt acagatgccc agcgtttaga
     1501 tacaattgac cctcaaacta cgaagcgcta catgcaccac tacaacttcc cgccatattg
     1561 tactggcgaa gttggccgca tgggtagccc aaagcgcagg gaaattggtc atggtgcgct
     1621 agctgaaaaa gcgctcgtac cagttcttcc tagcgaagaa gaattcccat atgcaattcg
     1681 tgtagtaagt gaaattatgg agagcaacgg ctcgtcttcg atggcttcta cttgcggatc
     1741 aactttggct ctcatggatg ctggcgttcc aatcaaacgt cctgtatctg gtattgcaat
     1801 gggtcttatc aaggaaggcg acgatgttgt aattctctct gatatccaag gtcttgagga
     1861 ctttttgggt gatatggact ttaaagtttg tggtaccgag cagggtatta cagcacttca
     1921 aatggataat aaggccaaag gcttgagtgt tgatattttg agtgctgcgc taacccaagc
     1981 aaaggaaggc cgcgctttca tcctcgatca tatgctcgaa gcaattgctg tacctcgcag
     2041 cgagatgaaa gagcacactc caaagattga aactatcaag attgacaagg ataaaatccg
     2101 cgacgttatt ggcaagggtg gcgagatgat tcgcagcatc caagaacaaa ctggtgctga
     2161 gattgaaatt ctcgaagacg gaactactcg tatttttgcc gctatgaaat ctgttggcga
     2221 tgctgccaaa gagatgatta aaaacatcgt aaaggagcca gaagttggcg aagagtacaa
     2281 cggcgaagtt gttgcaatta aagacttcgg cgcttttgtt cgcttaactc ctggtaaaga
     2341 tggtcttttg cacattagca agatggctca aggaagagtt cgtcgtgttg aagatgtatt
     2401 gaatattggc gatgaggtta aagttaagat taccgacatc gatagaggcg gcaagatttc
     2461 gcttgataga attgataagc cgagcgtcga aggtggcgac ggcgttgaat atgacgttgt
     2521 tgacactgaa agccacggcg gatacgaggg aagagctaaa cgaagaagat aaacaaataa
     2581 aattaacgga gtttaaatgc agaaagaaaa taacaaaaaa ccgcaaaaga aaaataagca
     2641 aaatcgaaac cgaacacatc attataaaca tgttcaagta aataaggttc gtccgaagct
     2701 ttcgcgtggc aactttgatc aggaccaaaa caagaaacaa gctgctaagc gtggttctgt
     2761 ccaaaaagat aaaaacgcga agcttcgtgt tatcccgctt ggtggtcttg acgccattgg
     2821 cgataacatg actgttttcg agtgcgaaaa cggcatgatt ttagatgatg ccggaatgat
     2881 gtttccaagc gatgctcatc ctggcgttga ccttattttg cctgattaca catatgtttt
     2941 agaacatgct gacaaactaa aagctattgt tattactcac gggcacgaag atcatattgg
     3001 ctgcttgcca tacctcattc aagatttggg aaggcctgtg ccaatcttcg ctacgaagct
     3061 tgctattggt tttattaagg ccaagtttga ggaatttgat attaaaaata ttagctttaa
     3121 cgaaatcaaa cccggcgatg ttattaacac aggatgtttt caaatttcgt ttttccaggt
     3181 caatcactcg attcctggat gtgttggtat gtttattcaa actcccgctg gaaacatttt
     3241 gcatacggga gattttaagt tggatcaaac tccaatcgac ggcatgacaa ctgattttga
     3301 cttaatttca aaatttgcca agactggaat tgaccttctt atgagcgact ccactaacgc
     3361 aaatgttaaa gagttcactc catccgagag cacagttggc cctgaacttg aaagaattat
     3421 tcgcgatgca aagggcaaag ttattgttgc aaccttcgca agtcacattc acagagttca
     3481 gcaagtttgt gatgctgcaa ttaagtgtgg tagaaaagtt gttgtagctg ggcgttcgat
     3541 ggtccaaaac gtagagattg ccagaaagct tggctatttg catgtctctg atgaacacat
     3601 aattgatgct tatgaacttg acaatattcc ttcaagcgat gccgtaattt tgtgcacggg
     3661 aagccaaggc gagccgctta gcgctcttgc aagagtgtcg actgattccc ataaaagaat
     3721 taccgcagaa aagggtgaca ctgtaattat ttcagcgaca cctgttccag gaaacgaaaa
     3781 agcggtttcg gcagttgtaa ataatcttgc taagcttggt gtaaatgttg ttgataaaac
     3841 aattgcgcct gttcatgttt ctggtcatgc ggcatccgaa gagcttaaga tgatgatttc
     3901 gctttgcaat ccatatggct ttatgcctgt tcatggcgaa gctgttcact tgcttgctca
     3961 tgcaaagctt gccgaacaat gcggagttga tcatgacaag atcttcttgt gcgaaaacgg
     4021 ggatgttatt gagatgtcga cttctgggct tcgtcgatta gaaccagttc aaagtggtgt
     4081 tgtttatgtt gacggattaa gcgtagggga cacttcgcaa gatgttttga aggagcgtaa
     4141 cttccttggc gagggcggta ttgctgttat aagtgcggct attaattaca ggcaaaagca
     4201 aatccttggt cctgtttatg ttaatttaag aggtgtttca ggaggcgatg accctgattt
     4261 gcaagatgaa cttcgaaacg ctgtagttcg tgcacttaac aaagaactta aaaagagcac
     4321 tgataataat catattcgaa aagcggcgaa ggatgcactt ctttcgcttt tgtgagacag
     4381 gacaaatcaa agacctatgg tagttgtaaa tataattgag ctgtaaattt aaaaaggaac
     4441 taaaatggca caaaaaaagc agaacaaaga aaccaagaag acagcttcgt ttggaaaaca
     4501 aaaaagaaca aacaacggaa aagtaaaaca agttaaacat ggcgaggaaa ttatagactc
     4561 tcgtgcaaaa accgacatta tagcagttgt tttaattgtt gttggtgttg cgctttttgc
     4621 cgtttcgctt gtccctacag atgcgccggt tacttcgttt ttggttaaca tcttaagatt
     4681 tatcttcggt gttggtgtat ttattttccc gtttgcctta attatttgag gaggttcgtt
     4741 cttctttaga atggagaggc aaaagatttc tgtccgcgtt gcaattggtc ttagcttgat
     4801 cttcgttgcg attttgacaa ttatttcttt atgctcaact gctattgcaa tgaacaggcc
     4861 agagcaactt tttgaatacg aaactattat taactatggt ggcattatag gatcagcctt
     4921 gtcttgagtt ggttatacct tgcttggaaa tgttgtttcg ctgattctta tgattggcgt
     4981 gattattgca ggattgattg ttattgggct ttctatcaca acaattgtta cgcgcattaa
     5041 cgaacgccgc gaagatattg aacgcgagct cgaagaaaga aaacgtgcca gacttgctgc
     5101 taaagctgct cgcgaagccg aagaagcagg tgatgagcaa ggcgaagaga ccgttgcgat
     5161 cgatgctgct gttcaaaaaa ccatggcgat tgaagataat aaaggtaaaa agccgggtct
     5221 taagactcgc attatgggat ttaaggacca aaaagaaaaa gacaaagaag aatttgacta
     5281 tgatccaaat caaaacaaga caagacaaat tggttctccc gctggttcgg aaagcaagaa
     5341 aaaaacttct gaaacatcaa aagaccaggc atcgacacct tcggcaccaa aaacacaagt
     5401 gattgggcca aagcctatgg agggttttgt gcttccaagt tttgatatcc taaaagcacc
     5461 aagcgaaggc ggagaggtta ctcagtccga agagtcgctt aaggcaaaag ctgaaaaact
     5521 taaagagaca ttagaagaat ttgggatcaa ggcaaatgtt gttggatggg ttccaggacc
     5581 aactgttact ttgtttaagg ttgacttgcc ggctggtgtt cgtgttagca gaatcactgc
     5641 gctcaatgat gacattgctc ttgctcttgc tgctcctgga gttcgcgttt ttgcaccgat
     5701 accaggcaca acatatgtag gaattgagat tccaaacgat gacaggcaaa ctgttttact
     5761 cagtgaagtt ttgggcaaag cgaagaaggg tccacttcaa atggcaattg gtaaggacgt
     5821 tgagggcaat gctataacgg ccaatcttgc aaccatgcct cacttgctta ttggtggaac
     5881 caccggttca ggtaaatctg ttgctattaa cgccatgatt acttcaatac ttatgcgcgc
     5941 tacaccttcg gaagttaagc ttattttgat tgacccgaag cgagttgaat ttacccctta
     6001 taatggaatt cctcacttgt atgttccagt cgtaacagag cctaaagaag ccgcaagcgc
     6061 acttacatgg ggagtcgccg agatggaaag tcgacttaaa ttatttagcg aacacaaggt
     6121 tagaaacatc gaggcttata attccatggt tcaaaaaatg agtaaacgtg accgtgaagc
     6181 tgcggatttg tctgagcttc catatattgt aattgtgatt gacgagcttg cagacttgat
     6241 gatggctgtg ggtaaggaag ttgaattctc aatttcgcgt atagctcaac tcgcacgtgc
     6301 agctggaatt catttgattg tagcaactca gcgaccatct acaaatgttg taactggact
     6361 tatcaaggca aacataacta caagaatcgc atttaatgtt gcaagcggaa ttgattctcg
     6421 agtaatttta gacaccggag gtgccgagag ccttattggc ctgggcgatc ttcttttctc
     6481 taaaccagaa ttttcgaagc cacttcgtgt tcaaggttgt tttgttaccg aagacgagat
     6541 ctctgc
//
LOCUS       DBNCDC010000065         6387 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_6659_length_6387_cov_6.389924, whole genome shotgun sequence.
ACCESSION   DBNCDC010000065 DBNCDC010000000
VERSION     DBNCDC010000065.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6387)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6659_length_6387_cov_6.389924"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="AC04VQ_05345"
     CDS             <1..330
                     /locus_tag="AC04VQ_05345"
                     /inference="COORDINATES: protein motif:HMM:NF017555.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HIO9553566.1"
                     /translation="RICSIIKKAGSCPASADLSLLTDKFELDLCRLMDTFPEVVCKAA
                     KNRAPHNLVHYSQELASTFHQFYTNCRVVNDDADLQNARLALCDAARICLELTLSLLG
                     VSAPESM"
     gene            562..852
                     /locus_tag="AC04VQ_05350"
     CDS             562..852
                     /locus_tag="AC04VQ_05350"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIO9553567.1"
                     /translation="MVCCLLAACIVLANKLRKRKRLAKTSPFGMGDVKLLMVVCLFLG
                     PKLSFICLFTSCIAFLLFVFILNIVRETKITKFPFAPFILAGVCVASVIAIL"
     gene            899..1168
                     /locus_tag="AC04VQ_05355"
     CDS             899..1168
                     /locus_tag="AC04VQ_05355"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9553568.1"
                     /translation="MATSPEKRKTNLFFQRIAYSIGAFGNDVFYFALSTYLINFITSH
                     LIDTGDPELNNTLVAAITLIIMILRIVELLIDPFIGNAIDRTNTR"
     gene            1349..2344
                     /locus_tag="AC04VQ_05360"
     CDS             1349..2344
                     /locus_tag="AC04VQ_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HIO9553569.1"
                     /translation="MLPAMSFDSREREQTATFARVGSAIGGSLVGVVLMPIVLFFSLD
                     NSTGTGDNVGWFMFGFIVALTGIITALIVGIFTREKRDKLRENKEETKGIKAVFKVLL
                     RNDQLLWTAIGYIFYCTGIYLLNSLELYYFQYIMGYAEGFSILQTINMFVGIFAVSAF
                     PPLAKKFGRKKVLYLCFLSMFAGIALFAFAGSNLALVLVAAELFYIPQPIIFLVVLMT
                     ITDAVEYGQLVLGHRDESLALSVRPLCDKFGSAVSNGVVGQTAIIAGMTTGASAATIT
                     FEGALAFKIMMFAVPAVLLIIALVIFWRKVSLDEEMHNSLLKMLNLTWGLDESEK"
     gene            2317..3168
                     /gene="rsmI"
                     /locus_tag="AC04VQ_05365"
     CDS             2317..3168
                     /gene="rsmI"
                     /locus_tag="AC04VQ_05365"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIO9553570.1"
                     /translation="MGSGRIGKIIICSTPIGNLDDISKRLICSLNDADVIYAEDTRVT
                     SKLLNYLKVEKPIKRLDENIMDKHAAIVVDEAKFGEVVAYCTDAGTPGVSDPGLKLVE
                     AARDSGVEVEVVPGPSALISAYVASGFTNQSFFFGGFLPRKENAQINYLKELSNLNAV
                     LIFYESAKRLVSTLTNIAEVFPKTEISVCRELTKLHEEINVGNSLDVLEDYKNRESIK
                     GEIVICINNNPQNNSEDSDSGDTTFKLAQYLHQYGAKTGDISSALQEVFNISKNEAYE
                     IALNVKK"
     gene            3155..3910
                     /locus_tag="AC04VQ_05370"
     CDS             3155..3910
                     /locus_tag="AC04VQ_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIO9553571.1"
                     /translation="MSKNKKRLDQYLVDSGTFENAEDVKRAVIAHEVRVDTTYVDSAA
                     IMIELDDQDVAKSEIYIKNQKQFVSRGGHKLQGAIDEFNIDVSGKHCLDIGSSTGGFT
                     DCLLQAGAADVACVDVNYGQLAWRLRQDDRVKAFERLNIKEADPKTLGAPFDLVVTDV
                     SFISLASIAEVVASMCKAGSVFVGLIKPQFECRRGETVNGVVEDEAVRQRTIDEVVEA
                     YKNVGFSDPKIKQSPLRGPAGNIEYLIYTIKEA"
     gene            complement(3897..4550)
                     /locus_tag="AC04VQ_05375"
     CDS             complement(3897..4550)
                     /locus_tag="AC04VQ_05375"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIO9553572.1"
                     /translation="MENTFKKSLAVIGAVVVSALLAITICGCAKKSESKTSSSSDVKY
                     SADNFKQTDAVEIQVANYGTIKVGLDANAAPITVANFKQLVSDGFYNGLTFHRIISGF
                     MMQGGDPKGDGTGGSEKTIKGEFTENGVENLLAHTRGAISMARSSANDSASSQFFIVH
                     QTSSNNSKSLDGKYCCFGYVLEGMEVVDKIIADAKPTDSNGTIPAASQPKITSIKLL"
     gene            4624..4987
                     /gene="ssrA"
                     /locus_tag="AC04VQ_05380"
     tmRNA           4624..4987
                     /gene="ssrA"
                     /locus_tag="AC04VQ_05380"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            5363..5971
                     /locus_tag="AC04VQ_05385"
     CDS             5363..5971
                     /locus_tag="AC04VQ_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIO9553573.1"
                     /translation="MNQRDLTVTRGEYIYDSKLFSKSLLYLGIRKGDIIVCALPNLYQ
                     SLVIFKAANSIGAIVTFINQRSTSHQISKYVDLYKSKILFVFNKSSDYVKEINNCSIK
                     YIVNISEKKVDSRIYNKNLCDINSSRFIDFHSLPSLGGLWKSRAFDRFRHFNAKDDAL
                     ILYTSGSTGEPKNILFTNENIISSLVYLKASTHTKPYQKNNF"
     gene            6176..>6387
                     /locus_tag="AC04VQ_05390"
     CDS             6176..>6387
                     /locus_tag="AC04VQ_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553574.1"
                     /translation="MFSMKTYLSGGDFLSKNKSIKASNFFKSHGANEVMICNGSGNGE
                     LLGCCTNAMGYKYRPETVGKLVLGPR"
BASE COUNT         1823 a         1106 c         1276 g         2182 t
ORIGIN      
        1 agaatttgtt ctatcataaa gaaggcaggg agttgtccgg cttcggcgga tttgagtttg
       61 ctaactgata aattcgagct tgatttatgc aggttaatgg atactttccc tgaggttgtt
      121 tgcaaggctg caaagaacag agcccctcat aatttggttc actattcaca agaattggct
      181 tcgacattcc atcaatttta tacaaattgt agggttgtaa atgatgatgc cgaccttcaa
      241 aatgctcggc ttgctctttg tgatgcagca agaatttgtc ttgaacttac actaagtctt
      301 cttggcgttt ctgctccaga aagcatgtaa gctttgacta tagttgatca aatactcctt
      361 ttagtgctat ttgtcttttg cgttatagca tcactaaagg atgtcaaaac aacgaagatc
      421 ccaaactgaa ttcccgtaag cttatttact atttggcttt catcaatttt cgttaagtga
      481 attttaatta gcgctcacgt tctagacctg caaataattg aaatgttgtg aacatgcgga
      541 aagcaggttt taacttcggc tattgtttgt tgcttgcttg ccgcttgtat agtcctggct
      601 aacaaattaa gaaagaggaa acgcttggcc aaaacttcgc cgtttggaat gggggacgta
      661 aaacttttga tggtagtttg cttattcctt ggtccaaaac tttcatttat ttgcctattt
      721 acctcctgca ttgcattttt gctttttgtc tttatcctaa atattgtcag ggaaacaaaa
      781 attacgaagt tcccattcgc gccatttatt cttgcaggag tttgcgtggc ttcggttatt
      841 gctattcttt agattatttg ttatcatatc ttggtcaagt tttagagaga ggggccccat
      901 ggcaacaagt ccagaaaaga gaaaaaccaa tttgtttttt caacgcatcg catattctat
      961 cggtgctttt ggtaacgatg ttttttactt tgctctttca acttacttaa taaattttat
     1021 aacatcgcac ctgattgata caggcgatcc tgagcttaac aataccttgg ttgcagcaat
     1081 tactttgatt attatgattt tgcgtattgt tgagctttta attgatccat ttattggaaa
     1141 tgcaattgat agaacaaata cgcgttgagg tcactttaga ccttggattg ttattggtgg
     1201 tgttgttagt tcaattattt tgtgcgttat gtttacgaat ctgggtggct tggttcaaac
     1261 aaatccttga ctttacctcg taatttttgc tgttttatat atcaccatgg atatctttta
     1321 tagctttaag gatgttggat tttgaagcat gcttcctgcc atgagttttg attctcgcga
     1381 gcgtgagcaa actgctacat ttgctcgtgt tggctccgca attggcggtt cgcttgttgg
     1441 cgttgtctta atgcctattg ttttgttttt ctctcttgat aactctactg gtactggtga
     1501 caatgttgga tggtttatgt ttggttttat tgttgcctta actgggatta ttacagcttt
     1561 aattgttggt atttttactc gtgaaaagag agataaactt cgcgaaaaca aggaagaaac
     1621 gaagggtatt aaagctgtct ttaaagtttt acttcgaaat gatcaacttc tttggactgc
     1681 aattggatac attttttatt gcacaggaat ttatcttttg aattcacttg aactttatta
     1741 tttccaatac attatgggtt atgccgaagg cttttcaatc cttcaaacta taaatatgtt
     1801 tgttggaatt tttgcagtta gtgcgtttcc tccgcttgcc aaaaaattcg gtcgcaagaa
     1861 ggttttgtat ttgtgtttct tgtctatgtt tgcaggaatt gccttgtttg cttttgctgg
     1921 aagcaatctt gctcttgttc ttgtagccgc tgaacttttt tatattcctc aacctataat
     1981 tttcttagtt gttcttatga caattactga tgcagttgaa tatggtcaac ttgttttggg
     2041 gcacagagac gaatcgttag cgctctctgt tcgtccactt tgcgataaat ttggttctgc
     2101 tgtttcaaac ggtgttgttg gtcaaactgc tattattgct ggcatgacaa cgggtgcttc
     2161 agccgcaact attacatttg aaggagcatt ggcattcaag attatgatgt ttgctgttcc
     2221 tgctgtgttg ctaataattg cccttgttat tttctggcgt aaagtatcac ttgacgaaga
     2281 aatgcacaat agtctattaa agatgcttaa tttaacatgg ggtctggacg aatcggaaaa
     2341 ataataattt gctcaactcc cattgggaat cttgacgata ttagcaagcg cctaatttgc
     2401 tcgttaaatg atgccgatgt aatttacgcc gaagacacac gcgtgacttc caaactttta
     2461 aattatctta aagttgaaaa gccaatcaag cgcttggacg aaaacattat ggacaagcat
     2521 gctgcaattg ttgttgacga agccaagttt ggcgaagtgg tagcttactg caccgatgct
     2581 ggaactccgg gtgttagtga cccgggcctc aagcttgttg aagctgcacg cgattctgga
     2641 gttgaagttg aggttgttcc tgggccttcg gcactaattt cggcttatgt tgccagtgga
     2701 tttacaaacc agagcttttt cttcggaggt tttttgccaa gaaaagaaaa tgctcaaatt
     2761 aattatttaa aggaattgtc caacctaaat gcggtgctta ttttttacga aagcgctaaa
     2821 aggttggttt caacactcac aaatattgcc gaagtgtttc caaaaacgga aatttctgtt
     2881 tgcagagagc ttacaaaact tcacgaagaa attaatgttg gaaattcgct tgatgtgtta
     2941 gaggattaca aaaatcgcga atcaattaaa ggtgaaattg tcatttgcat aaacaacaat
     3001 ccgcaaaata actccgaaga ttcagattcc ggcgacacca catttaagct tgcgcaatat
     3061 cttcatcaat atggtgcaaa aacaggagac ataagctcag ccttgcaaga ggttttcaat
     3121 atttctaaaa acgaagccta cgagattgcc ttaaatgtca aaaaataaaa aaagactcga
     3181 tcaatattta gttgacagcg gaacgtttga aaacgccgaa gacgttaagc gcgctgtgat
     3241 tgctcacgaa gttagggtag acacaactta tgttgacagt gctgctatta tgattgagct
     3301 tgatgatcaa gacgttgcga aatctgaaat ctatatcaaa aatcaaaagc aattcgtctc
     3361 tcgcggaggt cataaacttc aaggcgcaat tgatgaattt aatattgatg tttctggcaa
     3421 gcattgttta gacattggct catctacagg tgggtttaca gattgtttgt tgcaagctgg
     3481 agccgctgat gttgcttgtg tcgatgtaaa ttatgggcaa cttgcatggc gtcttcgtca
     3541 agatgatagg gttaaagctt ttgagcgctt aaacattaag gaagctgatc caaaaacttt
     3601 aggagctcca tttgatttgg ttgtgactga cgtgagtttt ataagcctcg cttcgattgc
     3661 tgaagttgtt gcttcaatgt gcaaagcggg aagtgtcttc gttggtctta tcaagcctca
     3721 atttgaatgc agacgtggcg aaactgttaa tggtgttgtc gaagacgaag ctgtccgcca
     3781 acgcacgatt gatgaagttg ttgaagctta taagaatgtt ggtttttctg atccaaaaat
     3841 aaagcagagc cccttgcgag gccctgccgg taacatcgaa tatttaatct acactattaa
     3901 agaagcttaa tagatgtaat ctttggttgc gaagccgctg gaatagttcc attgctgtct
     3961 gttggtttag catctgcaat aatcttgtca acaacttcca tgccttctaa aacataacca
     4021 aagcaacaat attttccgtc aagtgacttt gaattattgc ttgaagtttg atgaacaatg
     4081 aaaaattgag agcttgcact atcatttgct gaagagcgcg ccattgaaat agctccacga
     4141 gtgtgagcca ataaattttc aacgccattt tcagtaaatt cacctttgat agttttttca
     4201 gatccgccag ttccgtcacc cttggggtct ccaccttgca tcataaaacc agaaatgatt
     4261 ctatggaagg ttagtccatt gtagaaaccg tcactaacaa gttgtttaaa attggctact
     4321 gtgataggag cagcatttgc atcaagacca actttaatag ttccataatt agcaacttga
     4381 atttcaaccg catctgtttg cttgaaatta tcagcgctat acttcacatc tgaagaagaa
     4441 cttgttttag attcgctctt ttttgcgcaa ccacaaattg taattgcaag aagagccgaa
     4501 acaactacag caccgattac agcaagtgat tttttaaatg tgttttccat gttatctcct
     4561 ttggttagtg cttgcaatta taacaaattt gcgctattat gcacgcttgc actttgaaaa
     4621 gatgggggcg accggtttcg acatgttacg tgactgttgc tctgcaggtc gtggtctcct
     4681 gatccacgtt aaataacagg tgcgtcaaaa ttaattgaca aatctaactc tggccgtttg
     4741 gccatggcag cataaacata agtctgcctg cctcgctgct tgactccttc taaggcagac
     4801 caggtatcat ttttagaagg atgctgcgct tggtgtagct ggtatacaag cgtttaagat
     4861 taaacccgca cttgtgcaat ttgtgtgtca gccacgtttt tgcactcgta ttaattgttg
     4921 actaagcttg tagaggagtt tcaggtatgt agtatggacc ggagttcgat tctccgcgcc
     4981 tccaccatct tgtatgctcg ggctgacgcc ctgcgcatag agctttcgct gcgaaataat
     5041 tatatccatc atatttaccc ttgttttttg actcgcgtac acagtacgcg tcgtcaaaaa
     5101 ggcgggcaaa tctgaaggat ataattattt ctcgctgtct ttatctgttt cttgaggtta
     5161 ttcaatttat ataatttatt attgtgttat catatataga aaatatatga ttaatatgcg
     5221 aaaaagtaca cgaatatatc cgtccaaaag taaactacac tataaaggtg ttacattcaa
     5281 ggataagcat ccttttgttc cgcatgtaaa tgtgtccaca gctatagatt ttattagtaa
     5341 aaatcataaa gattgacctt taattaatca aagagattta acagttacta gaggtgaata
     5401 tatttatgat tcaaagttgt tttcaaaatc tctattgtat ttaggtataa gaaagggaga
     5461 tattattgtt tgtgcattgc caaatttata tcaatcatta gtaatattta aagcagcaaa
     5521 tagtatagga gcaatagtta cattcattaa tcaacgttca acatctcatc aaattagcaa
     5581 atatgttgat ttatataaat ctaaaattct ttttgttttt aataagtctt ccgattatgt
     5641 taaagaaata aataattgta gtattaaata tattgttaat atttctgaaa aaaaagtaga
     5701 ttcaagaata tataacaaga atctgtgcga tattaatagt tcgaggttta tagactttca
     5761 tagtcttcca agtttaggag ggttatggaa gtcaagggct tttgatcgct ttcgtcattt
     5821 taatgcaaaa gatgatgccc taattttata tactagcgga tctacaggtg aaccaaaaaa
     5881 tatactattt acaaatgaga atattatttc ttctttagtt tatttaaaag cttctacaca
     5941 tacaaaacca tatcaaaaaa ataatttttg atgactaagt accatcccat ttatgtatcc
     6001 atatggtttt aattgctgtg ctatggttgc gttgcttggg ggctttggtt taagattagc
     6061 tccagacata tcaaaaacaa atttgaatta ctatttttcc aaagaatcgc atgttgtttg
     6121 tggatgtcca gctctattag atgtaatatt tgataatttg tcagatgacg tcagtatgtt
     6181 ctctatgaaa acatatttgt ctggtggaga ttttctttca aaaaataaat ctataaaagc
     6241 ttctaatttc tttaagtctc atggtgctaa tgaagtaatg atttgtaatg ggtctggtaa
     6301 tggagaactt ttgggttgct gtacaaatgc tatgggatat aaatatcgtc cagaaactgt
     6361 tggaaaactt gtacttggac ctagata
//
LOCUS       DBNCDC010000066         6297 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_6839_length_6297_cov_6.651874, whole genome shotgun sequence.
ACCESSION   DBNCDC010000066 DBNCDC010000000
VERSION     DBNCDC010000066.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6839_length_6297_cov_6.651874"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="AC04VQ_05395"
     CDS             complement(<1..387)
                     /locus_tag="AC04VQ_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase Cbb3"
                     /protein_id="HIO9553575.1"
                     /translation="MPSLITTIKYAYKKHIQPRRARIRHVLLTILVLLAVAAVLEVVV
                     FNFRTIVTAFNKPIDISQKMNLQQTADGRYVVSSSQNTIEIKDINEEVSSINLKMNKN
                     QGAQVFDVKINFTDSGHSTYFDTTEYT"
     gene            complement(397..1047)
                     /locus_tag="AC04VQ_05400"
     CDS             complement(397..1047)
                     /locus_tag="AC04VQ_05400"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006363092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIO9553576.1"
                     /translation="MNIVLLGGPGAGKGTQSEFLKTEFGMLHISTGDLLREAVANQTE
                     AGVKAKSFMDAGELVPDEVILGIMQEKFETSDLSNGLILDGFPRNTEQAVALDNMLDG
                     LSTQIDKAILIDTPNDIIIQRICSRRMCKSCGHIGSVLGMNAEDAAAYVCPDCGGEMY
                     QRDDDNEATVQNRIDVYMEKTAPLIDYYSKQNKLAKVDGSPSVPKEVFENLKAVLE"
     gene            complement(1184..2392)
                     /locus_tag="AC04VQ_05405"
     CDS             complement(1184..2392)
                     /locus_tag="AC04VQ_05405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIO9553577.1"
                     /translation="MNPLVIVPVIISSRNKKQGDSVLTTYDHATPMSQAGELPRLLDS
                     LRNVDYVSHVVILVVAENTITEPAAIKIERLTMQYPEFNTMVIGADELAVIHKRAEEL
                     GVPNVNKEIALTSYGAIRNVGLTIAQIFGFDSVIFLDDDEVVDDKDFMSKAIYGLGKF
                     TKSGVPIIVKTGYYLNRFGNYTSSHKATWYDRFWQKGRAFNSWINGAMKGPRLSRSNH
                     VCGGVMVIHKQAFTRLAFDPWVARGEDLDYMLNLRMYGSDIWFDNQLCVKHLPPKSPS
                     EGVRFRQDIYRWLYEYRKLEYSRTQIDLLQLRAQDLKPYPGIFLDHDIERRIKLTARL
                     RSFGRPDKKEYRAAAKAATGEANIFAQRNCKNYFEFQRIWPQFMDCIACDKNLQEQLE
                     GAGHNRRRAA"
     gene            complement(2447..3925)
                     /locus_tag="AC04VQ_05410"
     CDS             complement(2447..3925)
                     /locus_tag="AC04VQ_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013979385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HIO9553578.1"
                     /translation="MKNGFDNDKYIQLQAKSIRERIDKFGGKLYMEFGGKLFDDHHAS
                     RVLPGFQPDAKIKMLQELQEDVEILIVINALDIVSNKTRSDIDIPYSEDVMRLVDIFH
                     DMGFYVGSVVITRYAEQLEVDDYIGHLEQMGVKTYKHRTIEGYPYDIDHLVSDDGFGK
                     NEYIETKKPLIVVTAPGPGSGKMATCLSQLYHDYKKGIKSGYAKFETFPVWNLPLKHP
                     VNIAYEAATVDLEDTNAIDPFHLEAYGKTATNYNRDIESFPVLQEMMTRIMGSCPYKS
                     PTDMGVNMVGRAIVDNSVVCNASCGEIIRRYYNLAVKDMRKGNAKDQVAAVKILMKQV
                     GVDPEKFPLRQAALERERKTGHPAGAMMLPNGDIITGKTTDLLGCASSLLLNALKRVA
                     NVEDVEIIKDEAIEPISKLKTGVFHSKNPRLHSDETLLALSASSSSEPRAQEMINAVS
                     ELRGCDAFFSVILSNADEHLYRTLGINLCSEPKFERTNLYHR"
     gene            complement(3995..4945)
                     /locus_tag="AC04VQ_05415"
     CDS             complement(3995..4945)
                     /locus_tag="AC04VQ_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012548.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine-type phosphatase"
                     /protein_id="HIO9553579.1"
                     /translation="MFYSNSYQRTIATTQYFSTGMLPIANVDVVHKYALNQQDRTFLP
                     IQTFMNDTLKAQIMKEIEAMGGDGGLNGILQNLSEEFKTLEYALDFKDSEYAKENNIT
                     SFPLDDLKINIVADDELTCEGSLKLGNTASDAFKLQYYEEEDDAKTSFGKKLNYKQWQ
                     QIGKIADVYEKAMFGSHTAAVNAAHPMLLELQKDMNNPNRKFTFLCGHDSTILSVVTA
                     LGVDDYQLPNTISTLTPIGVKLVVEKWKNAAGEEFGTLSLVYASVDQLRHRSELNVDD
                     PPCEYQLSLDGLQKNADGLYKWSDIQNRFSERIAEYDKYK"
     gene            complement(5075..5356)
                     /locus_tag="AC04VQ_05420"
     CDS             complement(5075..5356)
                     /locus_tag="AC04VQ_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553580.1"
                     /translation="MSKFVKNSKLTKFTFVVLATFVVMIAITLCACSQNGGKQIDTSY
                     VTESQPTGMKTYDGYTLEHMDVLSRHNIRSPLTGSGSTLDKLTPHKWFD"
     gene            complement(5489..6214)
                     /locus_tag="AC04VQ_05425"
     CDS             complement(5489..6214)
                     /locus_tag="AC04VQ_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIO9553581.1"
                     /translation="MLAYICAKASIASTPAIVEGIQGIVGQVVHGVTTAIAEKMMQAM
                     SSIDMDFNISSDQMMQLAKLMSGSGSRSYEGNLADFGYADLENPVSIIIYPNDFYAKE
                     IISDKIDGYNDEQQESGHEEKVISYTDYAKLLMSSLTTVIDMITAVLVAFVAISLVVS
                     SIMIGIITYISVLERRREIGILRAIGARKRDIFNVFNAETFIIGLIAGFIGIGFTAIG
                     CIPANIIAHAAFNVQYDIAILPI"
BASE COUNT         1797 a         1381 c         1242 g         1877 t
ORIGIN      
        1 tgtgtattcg gtcgtgtcaa aatacgtcga atgcccacta tccgtgaaat taattttcac
       61 atcaaagacc tgcgcgccct gatttttatt catctttagg ttgatgcttg aaacttcttc
      121 gttgatatct ttaatctcga ttgtattttg agacgaagac acaacgtaac gtccgtcagc
      181 cgtttgttgc aagttcattt tttgactaat gtcaattggc ttgttaaaag cagtgacaat
      241 ggttcggaag ttaaacacaa caacttctaa aacagcagct acggcaagca aaaccaagat
      301 tgtcaaaagc acgtgacgaa tacgagcgcg ccgcggctgt atgtgttttt tataagcgta
      361 tttaattgtt gtgattaaac ttggcattta accttcttat tcaagaacag ctttcaagtt
      421 ttcgaacact tcttttggaa cggaaggcga accatcaacc ttcgcaagtt tattttgctt
      481 tgaataataa tcaatcagcg gagccgtctt ttccatataa acatcaatgc ggttttgaac
      541 ggtggcttcg ttatcatcat cgcgttgata catctctcct ccgcaatccg gacaaacata
      601 ggcggctgcg tcttcggcgt tcatgccaag aacagaacca atatgaccgc aagatttaca
      661 catgcgccta ctacaaattc gctggataat gatgtcattt ggagtatcaa ttaaaattgc
      721 tttatcaatt tgagtgctaa ggccatcaag catattatca agagcaaccg cttgttctgt
      781 gtttcgaggg aagccatcca agatcaaacc gttcgagaga tccgaagtct caaacttctc
      841 ttgcatgatt cctaaaataa cttcgtcagg aacaagttcg ccggcatcca taaaactttt
      901 agccttcacg ccagcttcgg tttgattagc aacagcttcg cgaagaagat cacccgttga
      961 aatatgaagc attccgaatt ctgtttttag aaactcgctt tgagttccct tgcctgcccc
     1021 tggtccacct aataaaacaa tgttcatgat aaatccttta atccgcaata tttaagtgaa
     1081 tttcactggt ctcgccagcg ctaacatttt gtgatttttt attttcagag cgagacatcg
     1141 cagccttcgc cgcttctaaa tcattttcgg cttgcatttc atttcatgca gcacgacgcc
     1201 tattgtgccc agcaccttcc aattgctctt gcaaattttt atcacaagca atgcaatcca
     1261 taaattgagg ccaaattcgt tggaactcaa aataattttt acaattgcgt tgcgcaaaaa
     1321 tatttgcttc gccggttgcc gccttcgcag ccgccctgta ttctttttta tccggacgac
     1381 caaaactacg aagccgagcc gtcagcttaa tcctgcgttc aatatcatga tcaaggaaaa
     1441 tgcctggata aggcttcaaa tcttgagcac gaagttgcaa caaatcaatt tgagtgcgcg
     1501 aatattcaag cttgcgatat tcataaagcc atctataaat atcttgacgg aagcgaacac
     1561 cttcgcttgg gctctttgga ggcaaatgct taacacaaag ctgattgtca aaccaaatat
     1621 cacttccata catacgaaga ttgagcatgt agtctaaatc ttcgccgcgc gcaacccacg
     1681 gatcaaaagc caaacgagta aatgcttgtt tgtgaatgac catgacaccg ccacaaacat
     1741 gatttgaccg tgaaagacga ggccccttca tcgcaccatt aatccacgaa ttaaatgcgc
     1801 gacccttttg ccaaaaacga tcataccaag ttgccttatg tgagctagta taattgccaa
     1861 aacggttcaa gtaataaccg gtctttacaa taattggaac gccggacttc gtaaattttc
     1921 caagaccata aattgccttg ctcataaaat ctttgtcgtc aacaacttcg tcatcgtcca
     1981 agaaaattac tgaatcgaag ccaaaaattt gcgcaatggt cagcccaaca tttcgaattg
     2041 ctccatagct tgtaagcgca atttctttat taacgtttgg tacgccaagt tcttcggcgc
     2101 gcttatgaat aacagccagc tcatcggcgc caataaccat tgtgttaaat tcaggatatt
     2161 gcatggtcaa gcgctcaatt ttgatggcag cgggttctgt aattgtgttt tcggcaacaa
     2221 ccaaaattac aacgtgacta acataatcaa cattacgaag cgaatcaagc aagcgtggaa
     2281 gttcaccagc ctggctcatc ggcgtcgcgt gatcataggt tgtcaagaca ctgtcgcctt
     2341 gtttcttatt tcgacttgag attattaccg gtacaataac taatgggttc atttttactt
     2401 ccttttttta gtatatttta tcgtaattta ggttctttgt aaaatgctac ctgtgataga
     2461 gattcgttcg ttcaaacttt ggctcactgc aaagattaat gcccaaagtt ctatatagat
     2521 gttcatccgc gtttgaaagg atgacgctga aaaatgcatc gcaacctcga agttcgctga
     2581 ctgcgtttat catttcttga gcgcgaggct cacttgaaga tgatgccgaa agcgcaagca
     2641 aagtttcgtc tgaatgaagt cgcgggtttt tgctgtgaaa aacgccggtt ttaagtttcg
     2701 aaattggctc gatagcttcg tctttaatga tttcaacgtc ctcaacattt gcaactcgtt
     2761 taagtgcatt tagcagcagc gaagacgcgc aaccaagcaa gtcagttgtt ttgccagtta
     2821 taatgtcccc gtttggaagc atcatagcac ctgctgggtg gccagttttt cgctcacgtt
     2881 ccaaagctgc ttgacgaagc ggaaacttct cggggtcaac accaacttgc ttcatcaaga
     2941 ttttgactgc agcaacttgg tcctttgcat ttcccttgcg catatcttta accgccaaat
     3001 tataatagcg gcgaattatt tcaccacacg aagcattgca aaccacgctg ttatcaacaa
     3061 tcgcacgacc gaccatgtta actcccatgt cagttggcga tttataaggg caacttccca
     3121 taattcgagt catcatttct tgaagaaccg ggaagctttc gatgtcacgg ttatagtttg
     3181 tagcagtttt tccataggct tccaagtgga aaggatcaat tgcattggta tcttccaaat
     3241 cgacggtcgc ggcttcgtaa gcaatattaa caggatgctt gagaggcaaa ttccaaaccg
     3301 ggaaggtctc gaacttcgca taaccagatt taataccctt tttgtaatca tgataaagct
     3361 ggcttaaaca tgtggccatc ttcccacttc cagggcctgg agcagttaca acaatgagcg
     3421 gcttcttcgt ttctatgtat tcatttttgc cgaaaccatc gtcactaacc aagtgatcaa
     3481 tgtcatacgg atagccttcg atagttctgt gcttgtaagt tttaacgccc atttgctcaa
     3541 gatgaccgat gtaatcgtca acctcgagct gctcggcata gcgcgtgata acaacacttc
     3601 caacataaaa acccatgtcg tggaaaatgt caaccaggcg cattacgtct tcggaatatg
     3661 gaatgtcgat gtcgcttcga gtcttgtttg aaacaatgtc taacgcgttg ataacaatta
     3721 aaatttcaac atcttcctgg agttcctgaa gcattttgat cttcgcatca ggttgaaaac
     3781 caggtaaaac gcgacttgca tgatggtcat cgaagagctt gccgccaaac tccatataaa
     3841 gcttaccgcc gaacttatcg atgcgttcac gaatgctttt agcttgtaat tgaatgtatt
     3901 tgtcgttatc gaacccgttc ttcataacgg gtctccatta tagcatactc aatggggccg
     3961 ggggcagtgg gagttaatta ttttttattt tagtttattt atatttatcg tattctgcga
     4021 tgcgctcgct aaacctattt tgaatgtctg accatttgta gaggccatcg gcattcttct
     4081 gtaaaccatc caagctaagt tgatattcac aaggtgggtc atcaacattt aattcgctac
     4141 ggtgacgaag ctgatcaacc gacgcataaa ctaaactcag agttccaaat tcttcgccag
     4201 ctgcattttt ccatttctcc acaacaagtt taactccaat tggagtaagc gtagaaattg
     4261 tgtttggaag ttggtaatca tcaacgccaa gagcagttac aactgacaaa attgttgaat
     4321 cgtggccaca aaggaaagtg aactttctgt ttggattgtt catatctttc tgaagttcta
     4381 gcaacattgg atgcgcagcg ttaactgcag ctgtgtgcga accaaacatg gccttttcat
     4441 aaacatcagc aatctttcca atttgttgcc actgtttata gtttaacttt ttgccgaagg
     4501 atgtcttcgc atcgtcctct tcttcataat attggagctt aaaagcatcg gaagcagtgt
     4561 tgccaagttt tagcgagcct tcgcaagtaa gctcatcatc tgccacaatg tttattttca
     4621 aatcatcaag cggaaagctt gttatattgt tttccttagc atattctgag tctttaaagt
     4681 cgagtgcata ttcgagagtc ttgaattctt cagaaaggtt ttgcaggata ccatttaggc
     4741 ccccgtcccc tcccatggct tcaatctctt tcataatttg cgcctttaac gtatcgttca
     4801 taaatgtttg tattggcaaa aaagttctgt cttgctggtt taaagcgtac ttgtgaacaa
     4861 catcaacatt tgcaataggt agcattcctg tcgaaaaata ttgagtggtt gcaattgttc
     4921 gttgataaga attgctgtag aacaaagctt cgtcaccttg aggaactcaa ttttcaggaa
     4981 tgtaatttaa cttctcaaga tacttgcgga agtactggcc catgattgtt tcgagttctc
     5041 caccttgaag cgaaagttca cttgactttc cagttcaatc gaaccacttg tgaggagtca
     5101 acttatcaag cgtagaacca gacccagtta gcggcgagcg aatattgtga cggcttaaaa
     5161 catccatatg ttcgagcgtg taaccatcat aagttttcat gccggtcggc tgggattctg
     5221 taacatacga agtatcaatt tgcttgcctc cgttttgtga acaagcgcaa agtgtgatag
     5281 caatcattac aacaaatgtg gctaaaacaa caaacgtgaa cttcgtaagt tttgaattct
     5341 taacaaactt agacatgtga tctcctttca aaaacacctt ttaagaattg atggcttcga
     5401 ctggatcctt tttcgaggcc gaagtcgaag gtatgaggcc agcgacaaat gttagaaata
     5461 cgctgataaa tataagaata attgcacctc aaataggcaa aatggcaatg tcatattgaa
     5521 cgttgaatgc agcgtgagca attatgtttg caggaatgca tccaatagct gtaaatccaa
     5581 tgccaatgaa accggcaata aggccgatga tgaaagtttc tgcgttaaag acattaaaga
     5641 tgtctcgctt gcgagcacca attgcacgaa ggataccaat ttctcgcctt cgttcaagaa
     5701 ctgaaatgta agtaataatt ccaatcatga ttgaagatac aacaagcgaa attgcaacga
     5761 atgcgacaag cactgcagta atcatatcaa ttacagtagt cagcgaagac ataagaagct
     5821 tggcataatc cgtgtaagag atgacttttt cttcgtggcc agattcttgt tgttcgtcat
     5881 tgtagccatc aatcttatcc gaaataattt ctttagcata aaaatcattt ggataaataa
     5941 ttatcgaaac tgggttttca agatcggcat aaccaaaatc cgcaaggttg ccttcgtaag
     6001 aacggctgcc agaaccactc ataagtttgg caagttgcat catttgatca gatgagatgt
     6061 tgaaatccat atcaatgctg ctcatggctt gcatcatttt ttcagcgatt gcggttgtaa
     6121 cgccgtgaac tacctgacca acaatgcctt ggattccttc aacaattgca ggtgtcgaag
     6181 ctatactagc cttggcgcaa atataagcaa gcatggtttg accagtttcg gtttcgaaaa
     6241 acttctcagg cgttggatct tctgtcgctg gttgcttcgc cgctcaagca acataag
//
LOCUS       DBNCDC010000067         6270 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_6896_length_6270_cov_6.647305, whole genome shotgun sequence.
ACCESSION   DBNCDC010000067 DBNCDC010000000
VERSION     DBNCDC010000067.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6896_length_6270_cov_6.647305"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="AC04VQ_05430"
     CDS             <1..182
                     /locus_tag="AC04VQ_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553582.1"
                     /translation="QFFMKNTLTLSFSIFKANKGFSFGLLLLFWFGVTLVVMSCVLPS
                     TIAYSLDRFVDDYNM"
     gene            207..404
                     /locus_tag="AC04VQ_05435"
     CDS             207..404
                     /locus_tag="AC04VQ_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553583.1"
                     /translation="MQSDVDGLKDIEGVDKIESQMAVETSIKIDEDTNKQMRMFTIED
                     DGFRKYYFYEKKDVEEGQTSV"
     gene            429..551
                     /locus_tag="AC04VQ_05440"
     CDS             429..551
                     /locus_tag="AC04VQ_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553584.1"
                     /translation="MDLHAGDKIEVNFPDGYKELQIGAVISNPECSSSVYDDTY"
     gene            645..2315
                     /locus_tag="AC04VQ_05445"
     CDS             645..2315
                     /locus_tag="AC04VQ_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIO9553585.1"
                     /translation="MFKDGLSVEQKDEAISKACDLLGNNVISTSIFERSKAKTIIDSN
                     KEALFQACELFPYMILVILLVCSSLFLYQTINNQKKKIGLLRALGYSTKMIIGLYIIY
                     ILIIFIVALIIGIISALIFDDYVVKEYMLYFAMPEIFYIVPNKTYILILALLITGILA
                     CLISAFGIANIDPSEAYSSTVVTTQPYNFKTPFLKRIHVFNKIAIAKLFKNKKRLLML
                     SFSIAACIILSFMSIACVHSKAKSNTSTFGPRFSYDLEIYFDDDSICNEITSLDEVTK
                     LEKSVVFTERLYRNGKDLTLQYNAIDQDSQLVVPLDINMNRVYSQDGIVLDDFMFKYF
                     DLNLGDTINVGGVDLEIKERACEYVNFIQYISFDTAKKLGYNTSNACFVKLKDNVDLN
                     DMFKKISEMKGFKYMKFLSHQEQQKYQHQRALDLVYYAIIFISIMIGLIIIVNMVAIS
                     VQERKFEYGTLIALGTDKSKFISMTLIENLVQYLIAAIVAFVPCYYLAKLVLDSLTAP
                     QQNFVFVDIPDVYIQSFGLALIYIIVGIVYTIFSISKINPATTLNIRE"
     gene            complement(2317..2571)
                     /locus_tag="AC04VQ_05450"
                     /pseudo
     CDS             complement(2317..2571)
                     /locus_tag="AC04VQ_05450"
                     /inference="COORDINATES: protein motif:HMM:NF024051.9"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
     gene            complement(2599..3120)
                     /locus_tag="AC04VQ_05455"
                     /pseudo
     CDS             complement(2599..3120)
                     /locus_tag="AC04VQ_05455"
                     /inference="COORDINATES: protein motif:HMM:NF018647.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
     gene            complement(3184..3390)
                     /locus_tag="AC04VQ_05460"
     CDS             complement(3184..3390)
                     /locus_tag="AC04VQ_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553586.1"
                     /translation="MDKCYEYIDYLSLHKYFGFYNDDDPSELDNYLGKNIEMDKFIKE
                     VVEMCDKVGAEKKTDKKINLSFDE"
     gene            complement(3625..3777)
                     /locus_tag="AC04VQ_05465"
     CDS             complement(3625..3777)
                     /locus_tag="AC04VQ_05465"
                     /inference="COORDINATES: protein motif:HMM:NF046388.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553587.1"
                     /translation="METNEFGLHEFIDWSKKVNAEVCMGMNFGTRGIEDACNLLEYCN
                     FEKGTY"
     gene            complement(3838..4071)
                     /locus_tag="AC04VQ_05470"
     CDS             complement(3838..4071)
                     /locus_tag="AC04VQ_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553588.1"
                     /translation="MQAKMHIDRNQTISVIDERIYGSFIEQLGRAVYEGIYDPGNKYS
                     DDNGFRRDVIDAINKLNVPIIRFPGGNFVSQYN"
     gene            complement(4074..5444)
                     /locus_tag="AC04VQ_05475"
                     /pseudo
     CDS             complement(4074..5444)
                     /locus_tag="AC04VQ_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            5622..>6270
                     /locus_tag="AC04VQ_05480"
     CDS             5622..>6270
                     /locus_tag="AC04VQ_05480"
                     /inference="COORDINATES: protein motif:HMM:NF015573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HIO9553589.1"
                     /translation="MLTIACGIVVTLALAVVALLIGNAIKNRVNFLQKYCIPSPVVGG
                     FIFMILIFIGKYFGVVSITFQTTFQTVFMVAFFTTVGLSADFRLIKAGGGLLLIYWLL
                     AATISALQGPFALFLGGLMDLEPAYSITAGAISMCGGHGAGAAYGGMIKEMGWPAATD
                     CALACATLGLICSVMLGGPLSRRLILKNNLKPDAIQTGPKKAAPKTREERRSSAMD"
BASE COUNT         1953 a         1095 c         1094 g         2128 t
ORIGIN      
        1 ggcaattctt tatgaaaaac actcttacat taagtttttc aattttcaaa gcaaacaaag
       61 gtttttcgtt tggattatta ttattatttt ggtttggcgt aacattggtt gtaatgtcat
      121 gcgttcttcc atctacaatt gcatattcgc ttgacagatt tgtagacgat tacaacatgt
      181 gacaagctac gatagtaaca gagcctatgc aatcagatgt tgatggcctt aaagatatcg
      241 aaggtgtgga caagattgaa tctcaaatgg ctgttgagac ttcaataaaa atagacgaag
      301 ataccaacaa acaaatgagg atgtttacca tcgaagatga tggctttcgt aaatattatt
      361 tttatgagaa gaaagatgtc gaagaaggac agacatctgt gtgaattaca ttctttcttt
      421 cttatgccat ggatcttcat gctggagata aaattgaggt taactttccg gacggatata
      481 aggaacttca aattggcgct gtaatttcta atccggaatg cagttcaagt gtttatgacg
      541 atacttattg atgtgaggat tttgattatg gctatttgta tatttcaaga tcagactttg
      601 ataaatttta taaaactagt ggattaagca atgaaagaag ctttatgttt aaagatggct
      661 tgtctgttga acagaaagac gaagccataa gtaaagcctg tgatttgctt ggcaataatg
      721 ttatatcaac aagtattttt gagaggtcta aagcaaaaac tattattgat tcaaataagg
      781 aagccttatt tcaggcttgc gaactatttc cttatatgat attggtcata ttattagttt
      841 gctcaagttt gtttctctat caaacaatta ataatcaaaa gaaaaagatt ggtcttcttc
      901 gtgcgctagg ctattctaca aaaatgataa taggtctcta tattatttat atcttaataa
      961 tatttattgt agctctaatt ataggtatta tttcggcgct aatttttgat gattatgtag
     1021 ttaaagaata tatgctttac tttgctatgc ctgaaatatt ttacattgtc cccaacaaaa
     1081 catatatttt aatcttagct ttgttgataa caggaatttt agcttgtttg attagtgctt
     1141 ttggaattgc aaacattgat ccaagcgaag catatagttc cactgttgtt acaactcaac
     1201 cttataattt taagactcca tttttaaaac gtattcatgt atttaataaa attgcaatag
     1261 caaagctttt taaaaataag aaaagattgt taatgctatc cttcagtatt gcagcatgta
     1321 ttattttgag ttttatgtca attgcgtgtg ttcatagtaa agcgaaatca aacacatcga
     1381 ctttcggtcc aagatttagt tacgacttag aaatttattt tgacgatgat tctatttgta
     1441 atgaaattac ttcgttagat gaagttacaa agcttgaaaa gagtgttgta ttcaccgaaa
     1501 gattgtatcg taatggtaaa gacttaactt tgcaatacaa tgccatcgat caagattcgc
     1561 aattggttgt tcctcttgat ataaatatga accgcgttta tagtcaagat ggaattgttc
     1621 ttgatgactt catgtttaag tattttgatt taaatcttgg agacacaata aatgttggcg
     1681 gcgttgattt ggaaattaaa gagagagctt gtgaatacgt aaactttata caatatataa
     1741 gttttgatac tgcaaaaaaa ttaggctata acacgtcaaa tgcttgcttc gttaagctta
     1801 aagataatgt tgatttaaat gatatgttca aaaaaatctc tgaaatgaag ggttttaaat
     1861 atatgaagtt tttgtctcat caagagcagc aaaaatatca acaccaacga gcgttggatt
     1921 tagtttacta cgccatcata ttcatatcca ttatgatagg cttgattatt attgttaata
     1981 tggttgctat ctctgttcaa gaaagaaagt ttgagtatgg aacattaatt gccttaggaa
     2041 cagataaatc taaatttatt tctatgactt taattgaaaa tcttgttcaa tatctgattg
     2101 cagctatagt tgcttttgtt ccatgttact atcttgcaaa attagttctt gattctttaa
     2161 ctgcgccaca acaaaatttt gtttttgttg acatcccaga tgtttatatt caatcatttg
     2221 gattggcatt gatatatata attgttggaa ttgtttatac gattttcagc attagtaaaa
     2281 ttaatcctgc aacaacacta aatattcgag aataaattat ttattttctt cgtaggcttg
     2341 acgaactgca cgagctaatt gatctgcaca acttgttggt ttaggtccgc aagtgtttcc
     2401 aagcaagtat tcttcaattt tttcaactgt tcaaccatca actaattttg ctatagcctt
     2461 aaggttgcca ttgcatccgc caataaactc tatatttgta ataatattgt cattgatatc
     2521 gaagtttatt tcaggtgcgc aaaccatcgt tgttttatag ttatatctca tttaaatcac
     2581 cttaaccctt atcatgtttc aggacagagg ttctaacgaa gcactaatcc tgttttcgca
     2641 tacatctaca gataaatttt gaagctccat tgaatgatct tcgttagttt gcttaacatc
     2701 gtagccgaag aactgagtag cctcaattaa tttgtctagt tttatgttat caatatttgt
     2761 tttaaattca attttttcat tttggttttt atttaccgca aaaattataa gctcgttttc
     2821 tttttcatta agcgttgcaa ttgcatcgag gtaggggact tcggcgaatt cacgcgattt
     2881 gtaagtctca acatcaacat tagcatcaag gcaaattcct ttaccaaaat tagaaacttg
     2941 catatatggg taataagtag attgaagtca tacgccattt tcgttagtca ttattggagc
     3001 tataacattt acaagttgcg ccaagcatgc aatttttact ctgtcacaat ttttaagaag
     3061 agtaatcaag agggatccaa caagtaacgc gtcttcgaag ttataaatat cttctaacat
     3121 atgtggggca tgttgccatc attcaatttt agtatcagct tcattactgt gatatcaaac
     3181 atttcattcg tcaaaggaca aattaatttt cttgtcagtt ttcttttcag caccaacttt
     3241 gtcacacatc tcaacaactt ctttaatgaa cttatccatt tcaatatttt taccaagata
     3301 gttatcaagc tcacttggat cgtcatcgtt ataaaaacca aaatatttat gaagtgacaa
     3361 gtagtcgata tattcatagc atttatccaa gacttcaagt tctcaatctc catatgtagg
     3421 aagtttacta tctgaacttc cacaagcaac aagctcaata ttaggatcaa tctgtcgcat
     3481 agcttttgct gtttcgcgcg ctaatacagc atattcataa gcagtttttt gacctatttc
     3541 tcacggtcca tccatttcat ttccaagaca tcatgtttta attgcaaatg gtttttcagc
     3601 gccattttta cgacgaagat cacttcagta tgtgcctttt tcgaagttgc aatattcaag
     3661 taagttgcat gcgtcttcga tacctcgagt gccgaagttc atccccatgc aaacttcagc
     3721 atttaccttt ttggaccaat ctataaattc atgaagacca aattcgttag tctcaattgt
     3781 gtgtcatgct atgtcaaggc gagtagggcg attttccttc ggacctattc cgtcaactca
     3841 attgtattgg cttacgaaat ttccaccagg gaagcgaatg attggaacat taagtttatt
     3901 aatggcatct ataacatccc tgcgaaaccc attatcatca gaatatttat ttcctggatc
     3961 ataaataccc tcataaactg cacgcccaag ttgctcaata aatgagccat aaatacgttc
     4021 atcaataaca gagatcgttt gatttctgtc aatatgcatt tttgcttgca tgtttattcc
     4081 ttaatttcgc ctctgcgtgc gattagctct tttttaattt gtggttcctt cttctcaaat
     4141 ggtctgtata gaagaattag taatccgcca actatgaaaa atattgctgg caagaaagat
     4201 acacagaatt gaattgcgtc aagcgatgct tgagtttgaa cggcatccgc aacatatcct
     4261 gcattgtcta atattaacga aggtacccaa gaacctaagc ctgaaccaac tttaatagca
     4321 aacgaacttc caattgctgt caagaatcct gcagcgcgaa taccatttcg ccactctcca
     4381 taatctactg tatctgcaat cattgcaaat ggtaaagtta ccgaaattcc tgttccaact
     4441 gatgcgatgc accagaatac agcaatcata atttcgtttc cgcctgcaaa attaacaaga
     4501 atttgcccag cggccgctaa aaacattcca aacattaata cgtgaacttt gctaaatttc
     4561 ttaaccaagt aaggaattgc aaccatacca ataatttgga acattactaa agcgtttagg
     4621 attgaagcaa aatctttatc gccaagatta tattgagtaa agtatacaac taccgaagtt
     4681 ctactcatgt ttccaagcca ataaaaaatg aaaactacaa caataataaa ccatggccag
     4741 tttgcagcag cagctttaat agatttaacg atgctaatac ctttctttgg cttgttaatt
     4801 tcacgactat caaaaaacgc aaacatcaga agtgcaaaac caataattgc aaatacaata
     4861 gcagcgttac gccagcctgc ttgctcactt ccttggccga gtaaattgac gaagctcagc
     4921 ataaaggttg ctgtaataaa gtaaccaatc ataccgccga tgcttcgtat actattcatg
     4981 cgaagacgtt gttggttatc atttgttaag tttggcaaaa ttgaagttat aggagttcca
     5041 attccagtat agctgatacc aaataatatg taagtgacaa gagctcacca gaacttcgct
     5101 tcggcatcga tatctggact taaaaacatc agcacgaaaa atattgcaaa cggcgctgac
     5161 ataatcagcc aaaatggtcg acattggcct cagcgagtat gtgtatgatc gatgataaaa
     5221 ccccagattg gagcatcgaa agcatccagg atacgaacaa ctaatagaat tgtccctgca
     5281 gcagcaaccg aaagtccaaa aacatctgtg taatagtaaa gtataaatgt tgttacagtg
     5341 gtatagagta agttgcctgc agcatcagta cttgcatatg caagcattcg atggaacggg
     5401 attttttgtt ttgcgtctac aaccttagat accttgctgg tcataattct cctttcacta
     5461 atggtgaaaa ttatacctta atatgttatt atatgcgagt taatcgttaa gaaaggaaga
     5521 ttatggattc tagtgatggt gtatttgaca ttatcaaatg agtcagcggc gattacaaag
     5581 accctgtagg taacatccta acaatttcaa acgacaatgg aatgctaacc attgcttgtg
     5641 gtattgttgt taccctcgcc cttgcagttg ttgctttatt aattggtaat gcaatcaaaa
     5701 atcgagtaaa tttcttgcag aagtattgta ttccatctcc tgtagttggt ggttttatct
     5761 tcatgattct tatttttatc ggaaaatatt tcggtgttgt aagtatcact tttcaaacca
     5821 cttttcaaac agtgtttatg gttgcattct ttacgacagt aggtttgtct gcagacttta
     5881 gacttattaa agctggtggt ggattgttgt tgatttactg gttgttggca gccacaatca
     5941 gtgccttgca aggacctttt gctttgttcc ttggtggact tatggacctt gaacctgctt
     6001 attcaatcac agcaggtgca atttctatgt gtggtggtca tggtgctggt gctgcttatg
     6061 gtggaatgat taaggaaatg ggatggccag ctgcaaccga ttgcgcactc gcttgtgcta
     6121 cattaggttt gatttgttct gtaatgcttg gtggtcctct ttcacgtcgt ttaatcttaa
     6181 aaaataatct aaagcctgat gctattcaaa caggcccaaa gaaagctgct ccaaaaactc
     6241 gtgaagaacg tcgttcaagt gctatggata
//
LOCUS       DBNCDC010000068         6230 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_6979_length_6230_cov_5.861053, whole genome shotgun sequence.
ACCESSION   DBNCDC010000068 DBNCDC010000000
VERSION     DBNCDC010000068.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6979_length_6230_cov_5.861053"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="AC04VQ_05485"
     CDS             <1..451
                     /locus_tag="AC04VQ_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553590.1"
                     /translation="FYACINLVGVAGTEKVLYSVDHVDHSWARPAAVDSSGKLTYGYF
                     TAPKTPEKANSTNDELKTDTNKLEQNEIAKNDQKNIKPKVEEDVKVESEASVETELQD
                     DSIVELENPLSGIDIELDNKIETGKLVVMTFFQSIFNGLTKIMLSLM"
     regulatory      490..625
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            646..1248
                     /locus_tag="AC04VQ_05490"
     CDS             646..1248
                     /locus_tag="AC04VQ_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIO9553591.1"
                     /translation="MKKNSFKIISILGVAVCLCFGVMLSACTSSGGGGPLELTDLSGR
                     TVRLEKPASKIAALSASDCEVLCAIGAKDTIIARGTYCDYPADIVSVKDVGSGTLTST
                     EELLAMKPDIVLMSKTGFTLDQVNAMESAGLKTLVNEANTFDDTYKYIELLAKLTGHE
                     NEGSKVVADMKNSINDFRDKTKGKEQKTVYFQLCMPEHGY"
     gene            1270..1467
                     /locus_tag="AC04VQ_05495"
     CDS             1270..1467
                     /locus_tag="AC04VQ_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553592.1"
                     /translation="MDEMAEILNCKNVFDDVQGWAEVSKEELEARHPDVTIRIDDDES
                     FTRPGPRLIDALDKMCKLVYG"
     gene            1460..2437
                     /locus_tag="AC04VQ_05500"
                     /pseudo
     CDS             1460..2437
                     /locus_tag="AC04VQ_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013219.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
     gene            2541..3203
                     /locus_tag="AC04VQ_05505"
     CDS             2541..3203
                     /locus_tag="AC04VQ_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIO9553593.1"
                     /translation="MLIGPNGAGKSTSVFALAKTVPYSGVIELNGKNIQDYKNTEYAQ
                     KIGFLSQKNLVAYDFSVAEIVSMGRYAHAEGMFKTLSNEDKSKVEEALEVCGLQDLKD
                     RSVQELSGGEVQRTFLAQVLAQDPEVLVLDEPTNNLDLVYQSQIFDLLKRWISAKDGT
                     IISVVHDLALARYFGTSAVLLNNGKVASKGNVKEVLSDKNLNEVYKMDVKAYFKKLYA
                     NF"
     gene            3520..5160
                     /locus_tag="AC04VQ_05510"
                     /pseudo
     CDS             3520..5160
                     /locus_tag="AC04VQ_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014900.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
     gene            5188..5604
                     /locus_tag="AC04VQ_05515"
     CDS             5188..5604
                     /locus_tag="AC04VQ_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013399.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="HIO9553594.1"
                     /translation="MPDGRRSGEPLSPGANPAYGAEEKGLLASLNSLTKLPYKFALDG
                     ISNTQTINPSALGNNHDERVANLCDILDGYFDQGAHHLNVNVFGVEKLKDAMEHPEKT
                     EYANFTIRVSGYAVKFIDLTREQQLDVIARTCHEKL"
     gene            5608..6111
                     /locus_tag="AC04VQ_05520"
                     /pseudo
     CDS             5608..6111
                     /locus_tag="AC04VQ_05520"
                     /inference="COORDINATES: protein motif:HMM:NF024747.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
BASE COUNT         1842 a         1160 c         1412 g         1816 t
ORIGIN      
        1 gttctatgcg tgcattaatc ttgttggcgt tgctggaact gaaaaggttt tgtatagcgt
       61 tgatcacgtt gaccatagtt gggcaaggcc tgcggcagtt gatagcagcg gaaagctaac
      121 ttatggctac tttacagctc caaagacgcc tgagaaagct aatagcacta acgatgaact
      181 taagaccgat acgaacaagc tagaacagaa cgaaatagct aagaacgatc aaaagaacat
      241 caagccaaag gtagaggaag atgttaaggt cgaatctgag gcaagtgttg agactgaatt
      301 acaagatgac tcaattgttg agctagaaaa tccattaagt ggtattgata ttgagcttga
      361 taataagatt gaaactggaa agcttgttgt tatgacattc ttccagagca tctttaatgg
      421 tcttactaag ataatgctct cgcttatgta atacgttcgt cacaaaaatt taaaggcccg
      481 aattaacgtt cgggcctttt ttgtttatgc tataatatac gtgctttaag caatagggga
      541 atgcggtgag aatccgcagc aacagccatt accgtatttg acgaaagtca taagtcggga
      601 tacctactca gacatatgga agcttttggg taaaggagag caattatgaa aaaaaattct
      661 tttaaaatca tttctatttt aggcgttgca gtttgcttgt gctttggtgt tatgctaagc
      721 gcatgcacgt catctggagg gggcggtcca cttgaattga ccgacctttc aggaagaact
      781 gtgaggctgg aaaagccagc ttcgaagatt gctgcattgt cggcttcgga ttgcgaagtt
      841 ttgtgtgcca ttggtgccaa ggatacaatt atcgcccgtg gaacttattg cgattatcct
      901 gctgatatcg tgtcggttaa agatgttggt tctggtactt taacaagcac cgaagaactg
      961 cttgcgatga agccagacat tgttttaatg agcaagaccg gttttacgct tgatcaggtt
     1021 aatgcaatgg agagcgcagg ccttaaaact ctggtaaacg aagctaacac cttcgatgac
     1081 acttacaaat acattgaact cttggcgaag ctcactggtc atgaaaatga aggttctaag
     1141 gtcgtcgcgg atatgaaaaa cagtattaat gatttccgtg ataagacaaa gggcaaagaa
     1201 cagaagacag tttattttca actttgtatg ccagaacatg gatactgaac agccggaaaa
     1261 aataccttca tggacgagat ggcagaaatt ttaaattgta aaaatgtttt tgacgatgtt
     1321 caaggatggg ctgaagtttc taaggaagag cttgaggcaa ggcatccaga tgtgaccatt
     1381 cgtattgatg acgatgaaag ttttacaagg cctggacctc gcttaattga tgcattagac
     1441 aaaatgtgca agcttgtata tggctaaatt tccgaagaca ctttacttta tattagcaat
     1501 tgcactaaca attgttgtat ttgttatttg tgtttcgctt ggaagcacag gttttgctat
     1561 tgaaggcaac atcgtgagca gtattagact gccgcgagtt attgccgttg cgcttattgg
     1621 cgcatcgcta tctgtctgcg gagcggctat gcaaggactg ctccgcaacc ctttggctga
     1681 cggcacaacc ttgggtgtgt cttcggggtc ttcgctggga gccgtaattg gtatcgtgag
     1741 ctttagctca cttttttcac aatatgatcc gctaaacgga attttttccg ttttacttgc
     1801 aatggcggga ggcctgttga gtttaatatt tatcttgtct ctcgcacaaa aagtagatgc
     1861 aagattaagt acgaacacca ttatccttgt tggagttgtg ttttcgatgt tcgcaagcgc
     1921 cctgattagt ttaatagtcg ccttgtttcc cgaaagcgca aaaaccgtta tgttttgaac
     1981 gatgggtagt accgcagctg cttcgtttga agattgcatt ttacttggag ctgtttttat
     2041 tgtgtgtgcc agcgttttgc tatttaaggc aaacgagctt aacacatttg ctcttggcga
     2101 agaaaatgca agtcatcttg gggttaatgt taaacgagat aagttgataa ttatgattgc
     2161 tgtttcagtt ctgattggaa cttgcgttgc ggtttcgggg acaattggct tcgttggtct
     2221 tattgttcct catattagcc gtatgattgt tggcgctaat cacaaaaagc tgatgccatt
     2281 tacgatattt ttcggcgcga catttttgat gcttgcggac ttgctgagca gaataattat
     2341 cgccccgaag gagttgccga ttggcgttat aacttcgctg attggtgcga ttgtcttcgt
     2401 ttcgattttt attagaaaca ggaaaggttc taaataattt gccaagcaaa attttaaaca
     2461 tatcagattt aacagtaagt tttggtaaaa gcaaaatctt ggacaaggta tctttctctg
     2521 tcagcaaggg cgattgatgt atgttgatag gacctaacgg agcagggaag agcacctcgg
     2581 ttttcgcctt agcaaaaact gtcccataca gtggcgttat tgaactcaac ggcaaaaata
     2641 tacaagatta taaaaataca gaatacgctc aaaagatagg atttctttca caaaagaatt
     2701 tggttgccta cgactttagt gtagctgaaa ttgtttcaat gggtcgctac gcccacgccg
     2761 aaggtatgtt caaaacgctt tcaaacgagg ataaatctaa agttgaagaa gcacttgaag
     2821 tctgcggttt gcaagacctc aaagatagat cagtccaaga attgtcgggc ggcgaagtcc
     2881 agcgcacatt cctggctcaa gtcttagcgc aagaccctga agtcctcgtc cttgacgagc
     2941 ctacgaacaa tttggatctg gtttaccaga gccaaatttt cgatttgcta aaaaggtgga
     3001 taagcgcgaa agatggcaca atcataagcg tcgtccacga cctcgctctt gcgcgatatt
     3061 tcggcacatc tgccgtcctc ttaaataacg gaaaagttgc gtctaaaggg aatgttaaag
     3121 aagttctttc agacaaaaac ttaaacgaag tatataaaat ggatgttaag gcatatttca
     3181 aaaagcttta cgcaaatttt taacattata ttgtgtattt ttctaaaagt tttgcatata
     3241 atccacaaaa tgtagttatt tttttatttt tttgtgctaa tatacgccat agttatggta
     3301 aaagcacaaa aacaacataa aaccaagaaa aagaccagtt cagcgaccaa aaatgaccct
     3361 gcctttgctt gaagagaatt caagggtaag gactgaaaaa gcaaaattga cgtctatgat
     3421 tttattcaaa acaattatac gccctatgat ggcgatgctt cgtttttaaa aggcaccaca
     3481 gatgcaactg atagactttg gtgaaaagtc caagaacttc aacgcaaaga gcgtgctaac
     3541 ggtggtgttt tggacatgga cacggatatt gttagtgaca tcaattcaca taaaccggga
     3601 tacattggca aaggaacgaa gactatcgaa aagattgtcg gtcttcaaac tgacaagccg
     3661 ctaaaacgtg catttatgcc ttatggtgga attcgcatgg ctgaaggttc gcttgaagaa
     3721 tatggttata agcctgaccc tgctcttcat gagattttta caaagtatca caaaactcac
     3781 aacgatggcg tttttgatgt ttacagtcgt gaaatgctta acgcaagacg caataaaatc
     3841 ttaactggac ttcctgacac atatggtcgt ggccgaatag ttggtgatta caggcgagtt
     3901 gctctttatg gtattgatag actcatcgaa gaaaagcttc gtgattttta tgcaagcgaa
     3961 gatgacagca tgttggatga gactgttcgt cttcgtgagg aaattgcaat gcaacttgat
     4021 gctttagaag gcatgaagaa gatggcaaaa agctacggtt ttgatatttc gaagcccgcg
     4081 cgtgatgctc gcgaggcaat tcagtggctt tattttggtt atcttgctgc aattaaaact
     4141 caaaatggtg ccgcgatgag catcggtaga atttgcacat ttatcgatat atacattcag
     4201 cgcgatttga aatctaagaa gcttagcgag aaacgtgcgc aagaacttat tgatcattta
     4261 attttgaagt ttagacttgt taaatttgcc cgtattgcca gctataatga gcttttcagt
     4321 ggcgaccccg tttgggcaac tcttgacctt gctggtgtag gcgcggacgg aaggcacatg
     4381 gtcacaaaga ccgattttcg tgttttgcac actctcgaga acatgggtgc agctcctgag
     4441 ccaaatatta ctgtattgta ttcttcaaag ctgcctcaaa acttcaagga atacgcttcg
     4501 catattgcaa ttacaacaag ttcaattcag tttgaaaacg atgatattat gcgtcctgtt
     4561 tgaggcaacg attatgcaat ttgttgctgc gtttcggcaa catctacagg taaggaaatc
     4621 cagttcttcg gggctcgcgc taatttggct aaggctttaa gttatgcaat taatggtgga
     4681 tatgacgacg tgatgcacaa gcaagttggc cctgaataca aaccaattaa aacgaagtat
     4741 ctccactatg acgaagtaat tgagcgctac gaaaagatgc ttgactggct tgccaacctc
     4801 tacgttaaaa cgctcaacac cattcattac atgcacgata aatattatta cgaagcagct
     4861 gagatggcct taattgacac tgatgttcga agaacttttg caactggaat tgctggtttt
     4921 tctcatgttg tagattcgct ttcggcaata aaatacgcca aggttaagac gatccgcgat
     4981 gaaaatggtc atgttgtaga ttatgaaact accggcgatt ttcctcgtta tggaaatgat
     5041 gatgacaggg cagatgacat tgctgtttgg ttgctaaaaa ctttcatggc caaggttgca
     5101 agcaataaga cttatcgaaa ttccacgcca acaacttcaa ttttgacaat tacttcaaat
     5161 gttgtttatg ggaaggctac cggcaatttg cctgacggac gacgaagcgg agaaccgctt
     5221 agtccgggtg ccaaccccgc ttatggtgcc gaagaaaaag gccttttggc cagcttaaat
     5281 tcacttacga agttgcctta taaatttgct cttgatggca ttagcaatac tcaaacaatc
     5341 aatccatcgg cgttgggcaa caatcatgat gagcgcgttg caaacctttg cgatatattg
     5401 gatggttact ttgatcaagg cgcccatcac ttaaacgtaa atgtttttgg tgtcgaaaag
     5461 ctcaaggacg ctatggaaca tccagaaaaa acagaatatg ccaacttcac aattcgcgtt
     5521 agtggctacg ctgttaagtt tattgatctt acgcgcgagc aacagcttga tgttattgct
     5581 cgaacgtgtc acgagaagct ttaatccatg ataaaggcga agctacattc aacagaaagt
     5641 tttggcgctg ttgacggttc gggcattcgc ttcgtaattt tccttcatgg atgtaacttg
     5701 cgatgcgcgt attgtcacaa tccggacaca tgagctatta agcatggatt tagcgaggtt
     5761 actccgaaag agctcattga ccaggcactt cgttttaagc cttattgagg tcgtcaagga
     5821 ggaatcactg ttagtggtgg tgagccgctg cttcaacttg attttttgat tgagcttttt
     5881 aagcttgcga agtccaagca catcaacact gcaatagata ctgccggtga accattcaca
     5941 aataaaggtc cgtggtttga taaattcaag gagttaatga agttcacaga tacggttttg
     6001 cttgatctaa aacatattga cgaagacaag cacattcgct tgaccggtaa aaccaacaaa
     6061 aatattatag agatgtttaa atatctaaac aaaattaaga agccaacttg acttcgttat
     6121 gttttggttc caggctggac cgacagcgaa ggagagcttc gtagggcttc ggagtttata
     6181 aagaagttta aaaatatcga aagaattgat gttttaccat atcatactct
//
LOCUS       DBNCDC010000069         6138 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_7152_length_6138_cov_5.351964, whole genome shotgun sequence.
ACCESSION   DBNCDC010000069 DBNCDC010000000
VERSION     DBNCDC010000069.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6138)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7152_length_6138_cov_5.351964"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            193..269
                     /locus_tag="AC04VQ_05525"
     tRNA            193..269
                     /locus_tag="AC04VQ_05525"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:227..229,aa:Ile,seq:gat)
     gene            349..424
                     /locus_tag="AC04VQ_05530"
     tRNA            349..424
                     /locus_tag="AC04VQ_05530"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:382..384,aa:Ala,seq:tgc)
     gene            complement(464..1195)
                     /locus_tag="AC04VQ_05535"
                     /pseudo
     CDS             complement(464..1195)
                     /locus_tag="AC04VQ_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012516.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
     gene            complement(1214..1798)
                     /locus_tag="AC04VQ_05540"
     CDS             complement(1214..1798)
                     /locus_tag="AC04VQ_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553595.1"
                     /translation="MFKNLLKVSAVLLVCVCALCACTMQTTNDCTKNAEENATDPNQF
                     PDGSPIDPWFQNSETVTMTSMGQKLVVPNLVANNISDEVITQNTQNIQQTIDSATNNG
                     GGIIYISGGTYSIGALFFKPNVNLYIEEGSSLKGSEDIYFYPLVETRIEGQTVLYYSA
                     LINADKCDNFKIAGKGAIDGNGHKAWRQYHLRRT"
     gene            complement(1815..2447)
                     /locus_tag="AC04VQ_05545"
     CDS             complement(1815..2447)
                     /locus_tag="AC04VQ_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553596.1"
                     /translation="MHLFLHDGKQEVKTHQRATALASFTSTTPHDFTGGMNDGTNDPE
                     VRWTSYNGDNVVKNPWVEYDFNEPVCFNGFRIWWYDDNGGVRTPDGFTFDVYKKQNEG
                     EVVASLSSKDATSVSNEGSFITYMFPEAYEGDLIRMTLQNEVSAHAAGIVEWELIDAT
                     PQPPTPDPVDPGSSGTPQTGDNAWLAFAALLLLTAAVIATRKMAYNKISK"
     gene            complement(2493..2855)
                     /locus_tag="AC04VQ_05550"
     CDS             complement(2493..2855)
                     /locus_tag="AC04VQ_05550"
                     /inference="COORDINATES: protein motif:HMM:NF044298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553597.1"
                     /translation="MVSPTVKDGYLVISGRSWSSAGTKIEFDFDMPLKYETTDSRVVA
                     NQGLVALRHGPLFYCAEECDHPDFDYTKASISQGGECNFEEVKPLDGMKDDYGVCSVS
                     VLNHAGKLITPNGDQDIT"
     gene            complement(2889..3101)
                     /locus_tag="AC04VQ_05555"
     CDS             complement(2889..3101)
                     /locus_tag="AC04VQ_05555"
                     /inference="COORDINATES: protein motif:HMM:NF044297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553598.1"
                     /translation="MLQVGGYIYNTTSNAIYVNQYISNNATFSFGDYENTNVQMKSNL
                     PYEHNGEITITPQTTGSFDVYLRMPK"
     gene            complement(3099..3428)
                     /locus_tag="AC04VQ_05560"
                     /pseudo
     CDS             complement(3099..3428)
                     /locus_tag="AC04VQ_05560"
                     /inference="COORDINATES: protein motif:HMM:NF019556.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
     gene            complement(3444..3626)
                     /locus_tag="AC04VQ_05565"
     CDS             complement(3444..3626)
                     /locus_tag="AC04VQ_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553599.1"
                     /translation="MDDIILGKREYDQDHKPVSEQMEAVGHAVRAQYLYTGMCELATI
                     DPTTYASKYDTALQAL"
     gene            complement(3639..4016)
                     /locus_tag="AC04VQ_05570"
                     /pseudo
     CDS             complement(3639..4016)
                     /locus_tag="AC04VQ_05570"
                     /inference="COORDINATES: protein motif:HMM:NF019556.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
     gene            complement(4239..4532)
                     /locus_tag="AC04VQ_05575"
     CDS             complement(4239..4532)
                     /locus_tag="AC04VQ_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553600.1"
                     /translation="MSIKKNFKFIIVGTIAVIAAALIIRIASVPKIAQAKQASEPKRA
                     VPFSEVNVNEGFMHNYIKLVICEVIPTAITQVESYNEEKDECGGMYNIKNCAN"
     gene            4628..5848
                     /gene="hutI"
                     /locus_tag="AC04VQ_05580"
     CDS             4628..5848
                     /gene="hutI"
                     /locus_tag="AC04VQ_05580"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HIO9553601.1"
                     /translation="MTTLIKNIGELATPVGAGKQSGAMQNDCVKRMKNAYILIDGSKI
                     IEVGEGESPSADKVIDAKGALVTPGLVDPHTHLVFGGYREKELAMKLKGMDYLAIHNA
                     GGGINSTVKATREASAEELYAKASEHLDFMLTNGVTSVEIKTGYGLDTENEIKMLQVI
                     KKLKTNHKVDIAITFMPLHSLPDDFEGSTSDYVKYAIDEMLPAVIAEGGAEFIDVFCE
                     KDIFTADECKQVLDAGKAAGLIPKCHTNEIERIGGVEMATSIGAISMEHLIVANSEDI
                     NVMKDFDTIACLLPATSFYLGSTYALAREMIDNNVAVAFGSDFNPGSCPNNNFVLAMN
                     IGCLKYKMTPEESLCAATLNAAAAINRAKDFGSIEAGKQADILIWNANNLDYIFYRFG
                     QNLVSRVIKSGETI"
     gene            5863..>6138
                     /locus_tag="AC04VQ_05585"
     CDS             5863..>6138
                     /locus_tag="AC04VQ_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553602.1"
                     /translation="MENAKIDECMQIKLDYELPEYPKFEEGIRRAPKRESKLSKADKV
                     LAVQNALRYIHPDHHEKMAKEFAEELEATGRIYGYRFRPEGKLYGKPI"
BASE COUNT         1675 a         1226 c         1336 g         1901 t
ORIGIN      
        1 attcgccatg atccatccga ccttgattgc caaatgatca gttacttgcg tatgatgaaa
       61 ttaccttttg aaattcattt tacaaaagaa gataaactca gcaaacaaaa agcaaattcg
      121 caaaaaacaa aaatactcaa aatcgtgggt cagtaatggt tgctttcgct tataatacgc
      181 tcttgctgat acgggctcgt agctcagttg gttagagcgc acgcctgata agcgtgaggt
      241 cgctggttca aatccattcg ggcccaccat tgaaattgtg ataaacgctc cactgtgagc
      301 ttaagccggt ggggcgttta ttgacgaaag tgtcagcagc ctttatgtgg ggcattagct
      361 cagctgggag agcgctggct ttgcaagcca gaggtcaggg gttcgatccc cctatgctcc
      421 accacgaaaa attcgcgaag cgaatttttc gcatcttaat ttcttacagt aaattttcgt
      481 tgccgggttc tttgtaggtt acttcgttat ttttaaagca gaagtttttg catatgtatt
      541 catcatcgtt gatttctaca tgatagtaag tttcaacatc cattttgcaa tttctaattg
      601 ttagatgatt accgatagaa attggcttgt cagttcttcc ttgcatatcc atgaactgat
      661 aataaggacc aacgtagagg aaattgtttg cggtgcccgt gatgttttct accgtcatat
      721 attcatagac agatggcgtg tctgctctaa acttcaatct aaacattttg tctggatggt
      781 caacagtgca gtttcgcaaa ataatatttt tgttgcggac agattctgaa ccacatgtaa
      841 aaatgctcgc gcaatatcca aacttgcaat cttcaacaat tatgttttcg ttgaagccgt
      901 tggttggatc ttcgtcagcc caagcacctt taccgccttt cattacaaca gcatcgtcgt
      961 ttacatgcat gtagcaattt ttaacgtgta catttgtgca agcatcaatg tctatgcaat
     1021 ccgtgctagc tggcttttca tcaagctgtg aaataatcga agcgcccaaa aactttacgc
     1081 ggtcgcattt ataaatatga gctgtccaat aaaatgagtc tttcaaggtt acctcggaca
     1141 cggttacgtt attgctgttt gacacatata gaaggcgagg cctctgctct tctttattag
     1201 tacactcagg gtctcaagtt ctgcgaaggt ggtattgtct ccacgcttta tgaccgtttc
     1261 cgtcaattgc gccttttcca gcaatcttga aattgtcgca tttatcagca tttataagcg
     1321 ccgaataata caggacggtt tgaccttcga ttcgagtttc taccaacggg tagaaataaa
     1381 tatcttcgct gcctttgaga gaggagcctt cctcgatata caaatttaca ttaggcttaa
     1441 agaaaagcgc gccaattgaa taggtgccac cgctaatata gattatgccg ccaccattat
     1501 tagttgcact gtcgatggtt tgttgaatgt tttgagtgtt ttgcgtgatg acttcgtcgc
     1561 ttatattgtt tgcaaccaag tttggcacaa caagtttttg acccatcgaa gtcatggtga
     1621 ctgtttcgct gttttgaaac cacgggtcaa taggcgagcc atccggaaat tgatttgggt
     1681 ctgttgcgtt ttcttcggcg tttttggtgc aatcatttgt tgtttgcatc gtgcaagcac
     1741 aaagcgcaca aacgcaaacc aacaatactg ccgaaacttt caataaattc ttaaacaaaa
     1801 taaactcgct ttatttattt acttattttg ttgtaggcca ttttccttgt ggctataact
     1861 gctgcagtaa gtagtaataa tgcagcgaag gcaagccatg cgttgtctcc tgtttggggc
     1921 gttcctgacg aacctgggtc aacagggtcc ggagtaggag gctgaggtgt tgcgtctata
     1981 agctcccatt caacaattcc tgcggcatgt gcactaactt cgttttgcaa agtcatgcga
     2041 attaggtcgc cttcgtaggc ttctgggaac atgtatgtta tgaagcttcc ttcgttactt
     2101 acgcttgtgg catctttact gctaaggctc gcaacaactt cgccttcgtt ttgctttttg
     2161 taaacatcga aggtaaagcc atcaggcgtg cgtactccgc cattgtcgtc ataccaccaa
     2221 attctaaacc cgttaaagca aacaggctca ttaaagtcat attcaaccca aggattttta
     2281 actacattgt caccattgta gctggtccat ctaacttctg ggtcgtttgt gccatcgttc
     2341 attccacctg taaaatcatg aggcgttgtg gaagtaaacg aagccaaggc cgtggcgcgc
     2401 tgatgcgttt taacctcttg cttgccatca tgtaggaaca agtgcatgcc cccaagcgtt
     2461 ctgttgcaac gagcataata ggggataagt cttcaagtga tgtcttggtc cccgttaggc
     2521 gttataagct ttccggcgtg attaagtaca ctcacagaac aaacaccata atcgtctttc
     2581 atgccgtcta gaggctttac ttcttcaaag ttgcactcac caccttgact aatcgaagct
     2641 ttggtgtaat cgaaatcagg atggtcgcat tcttcggcgc aatagaaaag tggtccatga
     2701 cgaagcgcaa ccaaaccttg atttgcaaca actctgctat cagtcgtttc gtacttaaga
     2761 ggcatatcaa agtcaaattc aatcttcgtg cccgccgaag accaggagcg cccgctaatt
     2821 acaagatagc catccttgac agttgggcta accacttcgc cgccaacttt aatagttgag
     2881 gtgttggctc attttggcat ccttaaataa acatcaaaac ttcctgttgt ttgtggcgta
     2941 attgtgattt cgccattgtg ttcatagggt aaattgcttt tcatttgaac gtttgtgttt
     3001 tcgtaatcgc caaagctaaa ggtggcgttg tttgaaatgt attgatttac atatattgca
     3061 tttgaggtag tgttataaat gtagcctcca acctgaagta tggtgcgagt gaaattaggc
     3121 gggcagcaag ctgttccata ccatgctttt cggtcaaatg ctttagttgt gcaaatcctg
     3181 ttttcgtaat aaaatttgtc gccatcgaag ttaacgcatc ccaaaatatt attgtagagg
     3241 tcagtctcga tttgctctgc gtattgagaa ccatcgtaaa gaatactcat agaacggttt
     3301 caaagcatat tggcaacacc tgcgcatgtc tcacagtagg ataaatagtt tggtaaatag
     3361 taagcttcgg aaaatccttc attatgccca ctgctgtcac caacaccgcc agttatatac
     3421 tgctttttat ttgtaaggtc atctcaaagt gcttgaagag ctgtgtcata tttactagca
     3481 taggttgttg ggtcaatagt tgcaagctcg cacattccag tatataaata ttgtgcgcga
     3541 acagcatgtc caacagcttc catttgttca gaaacgggct tatggtcttg gtcatattca
     3601 cgtttcccta atataatatc gtccacagtt cttttgtctc aatcatgacc acgagcctcc
     3661 aagaaaaaag ctgctctgtc aatataatca ttagctttat ctacataagg tgacgtttta
     3721 atttctttgc aaagcttagc aagctttatc aatgcaagtt caatttcctc atgtccagga
     3781 atctgtatgc gtttttctgt gctattaggc ccaaaagtat ctacgatatg atttgcacta
     3841 ttaattgcaa catccaataa gcgagtgtct gggttggttg gatttttgtg aagctgataa
     3901 cgataatggg caaccgcggc ttcgataaaa tgtccttggc aataaagctc atggttatct
     3961 gtgtttgagt attttgtata tgttggctca agagaataaa agctatcaaa ataaccagga
     4021 tattgttggt ctgcttgttc aggagctcat tgatacaaag ggattcattc ttcaagctta
     4081 gttttaatgg ctgcctgtgc atctataatt tcttgatcac cgccatcagg tgtgtctaaa
     4141 gccatacaca ttgattctaa aactttatga acatctgaat caacgtattt tgcgccaagg
     4201 tgggttggtt tttccgttgg ttctgaacct gcttgatgtc aattagcaca atttttaata
     4261 ttgtacattc cgccacattc gtctttttct tcgttataag attctacttg cgtgatagct
     4321 gtagggataa cttcgcaaat aacaagtttt atgtagttat gcatgaagcc ttcgtttacg
     4381 ttcacttctg aaaagggaac tgcgcgttta ggttcgctag cttgttttgc ttgagcgatt
     4441 tttggaacag aagcaattct aattattagc gctgctgcaa taacagcaat agtacctaca
     4501 attataaatt taaaattttt tttgatagac attttggtct cctctcgaat ttttttggaa
     4561 attagcacgc gtgaattata ccaaatttgg tccgcggtgt cgggggtata gtgctataat
     4621 ttttttgatg acaactctca ttaaaaatat tggtgaactt gcaactcctg tgggagccgg
     4681 caagcaatct ggcgctatgc aaaacgattg tgttaagcgc atgaaaaatg cctacatatt
     4741 aatagacggc tcaaaaatta ttgaagttgg cgaaggcgag tctccatctg ctgacaaggt
     4801 tattgacgcc aaaggcgcgt tggtaactcc tggtttggtt gacccgcaca cgcaccttgt
     4861 cttcggcggg tatcgcgaga aggaacttgc gatgaagcta aaaggtatgg attacctggc
     4921 cattcacaat gctggtggtg ggattaattc gaccgttaag gctacgcgcg aagccagtgc
     4981 ggaagagctt tacgcgaagg cgagcgagca tcttgatttt atgcttacaa atggtgtgac
     5041 ttctgtcgag ataaaaacag gctatggcct tgataccgaa aacgaaatta agatgttgca
     5101 agttattaaa aagttgaaga ctaaccataa agttgatatt gctataactt ttatgcccct
     5161 gcattcgctg ccggatgatt ttgagggcag cacttctgat tatgttaaat atgcaattga
     5221 cgaaatgctt ccggctgtga tcgccgaagg tggagcagag ttcattgatg tattttgcga
     5281 gaaagatatt ttcacggcgg atgaatgtaa gcaagtactt gatgctggca aagctgcggg
     5341 cctaattcct aaatgtcaca cgaatgaaat tgagcgaatt ggtggagttg aaatggcaac
     5401 ttcgattggt gcaatttcta tggagcattt aattgttgca aacagcgaag acattaacgt
     5461 gatgaaggac ttcgacacta ttgcatgctt gcttcctgct accagttttt atctgggttc
     5521 aacttatgcg ctcgcacgtg agatgattga taataatgta gcagttgctt ttggaagtga
     5581 ttttaacccg ggttcgtgcc cgaacaataa cttcgttttg gcaatgaaca ttggttgtct
     5641 taaatacaaa atgacccccg aagagagctt gtgcgccgca actctaaacg cggctgccgc
     5701 aataaatagg gcgaaagatt ttggaagcat cgaagctggc aaacaggctg acatcttaat
     5761 ttggaatgcg aataatcttg actatatttt ttacaggttt ggtcaaaacc ttgtatcacg
     5821 agtgattaaa tcaggcgaaa caatttaata gtggagggga aaatggaaaa cgcaaagatt
     5881 gatgaatgta tgcaaattaa attagattat gaattgccag aatatccgaa atttgaggag
     5941 ggcattcgcc gtgctcctaa gcgcgagtcg aagctttcaa aggctgataa agttttggcg
     6001 gtgcaaaatg cgcttcgtta tattcatccg gatcatcatg agaaaatggc taaggaattt
     6061 gctgaagagc tggaagctac tggcagaatt tatggataca ggtttagacc agaaggaaag
     6121 ctttacggta agccgatt
//
LOCUS       DBNCDC010000070         5903 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_7689_length_5903_cov_6.056259, whole genome shotgun sequence.
ACCESSION   DBNCDC010000070 DBNCDC010000000
VERSION     DBNCDC010000070.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7689_length_5903_cov_6.056259"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="AC04VQ_05590"
     CDS             <1..752
                     /locus_tag="AC04VQ_05590"
                     /inference="COORDINATES: protein motif:HMM:NF014516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIO9553603.1"
                     /translation="KNLSFSYNKSEPVLRNVNLDFKPGKIYAILGQNGSGKSTLLHTI
                     SSILTNYEGKITVHLADKTINLAEIKHNERAKLISLVAQLNYGNSLNVYDSVLLGRKP
                     YINISPMMVDYEMTSRAIDKFKLNALTLKQTNELSGGELQSVGIARAFAQDTPIMLLD
                     EPTNNLDVKKQHELFDILKKLVKEKNLCVICVLHDINQAMKYANELIFMKNGEVIKSG
                     TKNIVAESLLNKVYDIEAEIITNKKSKYIII"
     gene            842..1039
                     /locus_tag="AC04VQ_05595"
     CDS             842..1039
                     /locus_tag="AC04VQ_05595"
                     /inference="COORDINATES: protein motif:HMM:NF017026.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIO9553604.1"
                     /translation="MVKQFADKVKDKSVLDIGCGTGVLYEHLSNAGATKIMSIDISEE
                     MIKLAKEKFPEGNFLVEDILT"
     gene            1070..1261
                     /locus_tag="AC04VQ_05600"
     CDS             1070..1261
                     /locus_tag="AC04VQ_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553605.1"
                     /translation="MYNVYPHLMDKQKLVEKVYSLLNENGTFIVAHGSSRDTINSHHS
                     SHAMEVSRKLLKAEEEAEI"
     gene            complement(1320..2918)
                     /gene="gltX"
                     /locus_tag="AC04VQ_05605"
     CDS             complement(1320..2918)
                     /gene="gltX"
                     /locus_tag="AC04VQ_05605"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015760509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIO9553606.1"
                     /translation="MTKENKEVRVRFAPSPTGMLHIGGARTAVNNWAYARATGGKFVL
                     RIEDTDLARSTEENTAIILRAMKWLGLDYDEGPEVEGPYGPYFQLQRLDLYKQAIDEL
                     WEKDAAYPCFCTKEEIDAKREKAIKEEGGYSGYDRTCRSIPRDEAQARMDAGEPFVWR
                     LKVPLEHAPIEFDDAVYGHMSFPADVMDDLVLTRGEDNIPTYNFAVVIDDSAMKITHV
                     VRGDDHLSNTPRQILIYEALGKPVPTFAHLSMILGPDGKKLSKRHGSTSVEEYQARGY
                     LPEALVNFLALLGWSLDDKTTIIPKDVLCKEFDLSRVTRKDSVFDETKLDWMNGCYIR
                     DMGSEKLAEISKEWFAKAVVQGKNESSQGSRIVESKTKEELKELDRQIKEFDEAEVEK
                     EINFVDENFDMFKKVTPLIIERLERLDQIPGKLSFMFWGPNLVLDENSKQKVFDKDSE
                     TAKQVLTSVLEIFEDENVKWDCKTLETKCQELCAKLDLKPRVFFQPIRVAVAGNMVSP
                     PLFECIELMKREDIIARIKFALSV"
     gene            2934..3179
                     /locus_tag="AC04VQ_05610"
     CDS             2934..3179
                     /locus_tag="AC04VQ_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553607.1"
                     /translation="MIIFPMIKETTDKAAEMTMQITAIPTQNAVGYIDINNDLSLKNQ
                     LLFAKNKECNQLNMLLKSTIAHTPKATKPTIIINPVH"
     gene            complement(3173..3595)
                     /locus_tag="AC04VQ_05615"
     CDS             complement(3173..3595)
                     /locus_tag="AC04VQ_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553608.1"
                     /translation="MTILSAGVVVCMSFPQITENLSRATAMVDTAHFTRDQLVKAALT
                     TRAFVAGEADKNEIYKTVQEINQEAKTEFSQYEGSDFAAVSDEYSIDHVAIKHLEDVK
                     SLFANVRIAFGICGFGALLFLILLFVFCGRSAFGRALQ"
     gene            3695..4897
                     /gene="metK"
                     /locus_tag="AC04VQ_05620"
     CDS             3695..4897
                     /gene="metK"
                     /locus_tag="AC04VQ_05620"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015539910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIO9553609.1"
                     /translation="MAETSNYLFTSESVTEGHPDKICDQISDAILDELLAREKQLELR
                     NFEDEHGKANVKRVRCACETLVSTGLVMLTGEIRTQAYVDIPRIARDVIRDIGYTHTE
                     YGFDAETCSVQNTVHEQSPDIAMGVDESVEAKAKSTDKYDLIGAGDQGIMFGYACNET
                     KTLMPMPIYLAHRLSERLATVRKDDIIPYLRADGKTQVTIEYKDGKPVLVNNVVLSTQ
                     HDEDIKEFELKRDVMDKVIKPVLDEAGIPTDKSIEYYINPTGRFVLGGPAGDSGLTGR
                     KIIVDTYGGAGHHGGGCFSGKDCTKVDRSASYMARYVAKNIVAAGLADKCEIQIAYAI
                     GVAHPTSIFIDCYDTNKEPIEKIEYAVSQTFDLRPAAIQDALDLWRPIYRKTSNYGHF
                     GRELPEFT"
     gene            4917..5141
                     /locus_tag="AC04VQ_05625"
     CDS             4917..5141
                     /locus_tag="AC04VQ_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553610.1"
                     /translation="MNFKDFYNFIKNKAPFSYILAILILAILIIITVFVVKEYRNFMN
                     NIQITISAEEELAKLEAEEQAKRDEELKKQ"
     gene            5217..5540
                     /locus_tag="AC04VQ_05630"
     CDS             5217..5540
                     /locus_tag="AC04VQ_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024919.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HIO9553611.1"
                     /translation="MIYQHPEFPAGCECIALTNMMNYYKFNLEKSTLIENYLEYSESD
                     FVNYYVGSPYTSGMGGVMMCPAISNLFNKYITVNPGPFKGFDITKRNFEDLFAYIERG
                     HPVQI"
     gene            5553..5777
                     /locus_tag="AC04VQ_05635"
     CDS             5553..5777
                     /locus_tag="AC04VQ_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553612.1"
                     /translation="MGGLGEVLSSFNNYLLYENTHSVLLTGFDRENGIVYIADSINGD
                     VYYNIETVRNIFEQQNSQAFVMISQEELDK"
BASE COUNT         1976 a         1126 c         1066 g         1735 t
ORIGIN      
        1 ttaaaaactt gagtttttct tacaacaaga gcgagcctgt gcttcgtaat gttaatcttg
       61 attttaagcc tggaaaaatt tatgcaatcc ttggccaaaa tggaagcggc aaatccacac
      121 ttttgcatac tataagtagc attttgacaa attacgaagg caagatcaca gttcatctag
      181 ctgataaaac tataaattta gcagaaatta aacataacga acgggcgaag ctaatctcgc
      241 ttgttgctca acttaattat ggaaattcac tcaatgttta tgattctgtt cttcttggaa
      301 ggaagccata tatcaatatt agcccaatga tggttgatta cgaaatgact tcacgcgcta
      361 ttgataagtt taaattaaac gcgctaactt taaaacaaac aaatgaactt tccggtggtg
      421 aacttcaaag tgttggaatt gcaagagcgt ttgcacaaga tactccgatc atgcttttgg
      481 atgaacctac aaacaatctt gatgttaaaa aacagcacga actttttgac attctcaaaa
      541 aattagttaa ggaaaaaaat ctttgtgtaa tttgtgtgct tcatgatatt aaccaagcaa
      601 tgaaatatgc taacgaatta atcttcatga aaaatggcga agttattaaa agcggcacaa
      661 aaaatattgt tgctgaaagt ttactcaata aagtatatga tatagaagcc gaaattatta
      721 ctaataaaaa gtcaaaatac ataattattt aatggacaat ctatacaaaa taaaagattt
      781 ttttaatgag attgcgtcaa cttgagatga caagaacgct gatgaacttg gcgcgaaaaa
      841 aattgttaag caatttgcag ataaagtaaa agacaaatct gttttagata taggctgtgg
      901 aactggtgtt ttatatgaac acttaagcaa tgcgggtgct acaaaaatta tgtcaattga
      961 catctccgaa gaaatgatta agcttgcaaa ggaaaaattc ccagagggaa actttttagt
     1021 tgaagatata ttgacttgaa aaactaacga taagtttgac gcaataataa tgtataacgt
     1081 ctatccacat ttaatggata agcaaaaact tgttgaaaaa gtttattcgc ttctcaatga
     1141 aaatggaacg tttattgttg cgcatggttc aagccgcgac actatcaatt cgcatcattc
     1201 ttctcatgca atggaagtaa gtcgcaaact tttgaaggcc gaagaagaag ccgaaatttg
     1261 acgcgcgaaa ttcgaagtca atattattcg cgataatgaa ctgtattact tttcaggaac
     1321 taaacgctaa gtgcaaattt tattcgtgca ataatatctt cacgcttcat taattctata
     1381 cactcaaaaa gtggcggaga taccatatta cctgcaacag caacacgaat tggttgaaag
     1441 aaaactcgtg gttttaaatc taacttagca caaagctctt ggcattttgt ttctaaagtc
     1501 ttgcaatccc acttaacatt ttcatcttca aagatttcaa gaacagaagt caaaacttgc
     1561 tttgcagttt cgctatcttt atcaaaaacc ttttgcttag aattttcatc caaaacaaga
     1621 ttaggtcccc agaacataaa gctaagcttt ccaggaattt gatccaggcg ttcaaggcgt
     1681 tcaataataa gaggtgtaac ctttttaaac atatcgaagt tctcatcaac aaagttaatc
     1741 tctttttcaa cttccgcttc atcgaattct ttaatttgcc tatcaagttc ttttagctct
     1801 tctttagttt tactctcaac aatacgtgag ccttgcgaag attcgttttt tccttgaaca
     1861 acagctttag caaaccattc cttagaaatt tctgcaagct tttcagaccc catatcacga
     1921 atatagcaac cgttcatcca gtccagttta gtttcatcga aaacgctatc tttcctggta
     1981 acacgagaca aatcaaattc tttacataaa acgtcttttg gaataattgt tgtcttgtca
     2041 tccaaagacc aacccaaaag tgccaaaaag ttcacaagtg cttctggcaa ataacctcgc
     2101 gcttgatatt cttctaccga agttgagcca tggcgcttcg atagcttttt accatcaggt
     2161 cccaaaatca ttgacaagtg agcaaaagtt ggaacaggct tgcccagggc ttcgtagatt
     2221 aaaatttgac gaggagtgtt tgataagtga tcgtctcccc taacaacgtg agtaattttc
     2281 attgcggaat catcaataac aaccgcaaaa ttataagttg ggatgttgtc ttcgccacga
     2341 gtcaaaacca aatcatccat aacgtcagct ggaaaactca tgtgcccata aacagcatcg
     2401 tcgaattcaa ttggcgcgtg ctcaagtgga acttttagac gccaaacaaa aggctctcct
     2461 gcatccattc tggcctgagc ttcgtcacga ggaattgagc ggcaagttct atcataaccg
     2521 ctgtaaccac cttcttcttt gattgccttt tcgcgctttg catcaatttc ttccttagtg
     2581 caaaaacaag gataagcagc atctttttcc cacagttcat caatggcttg cttgtacaaa
     2641 tccaagcgct gaagctgaaa gtaggggcca taagggcctt caacttctgg gccttcatca
     2701 taatcaagac caagccactt catcgcacgc aaaattattg cagtgttttc ttcggttgag
     2761 cgcgcaaggt ctgtatcctc aatacgaagg acaaacttac cacctgtagc acgagcatat
     2821 gcccagttat taacggcagt tctagcacca ccaatatgaa gcattccggt cggagatggc
     2881 gcaaaacgca cacgaacttc tttattttcc ttcgtcattt agatcacccc tatttgataa
     2941 tttttcctat gattaaggaa acaactgata aagcagctga aatgacgatg caaattaccg
     3001 ccattccaac tcaaaacgca gttggataca ttgatattaa taacgattta tcgctgaaaa
     3061 atcaactatt gtttgcaaag aataaggaat gtaaccagtt aaacatgctg ttaaaatcta
     3121 caattgccca cacacccaaa gcaactaaac ctaccataat aattaatccg gttcattgaa
     3181 gagcgcgccc gaatgcgctt cgtccgcaaa atacaaatag caaaattaaa aatagcaaag
     3241 cgccgaagcc acagattcca aacgcaattc taacgtttgc aaataatgac ttaacatcct
     3301 ctaaatgttt aattgcaaca tggtctatgg aatattcgtc cgaaactgca gcaaaatccg
     3361 aaccttcata ttgggagaat tcggtctttg cttcttgatt aatttcttga acagttttat
     3421 atatttcatt tttatcagct tcaccagcaa caaaagccct ggtagtaaga gctgctttaa
     3481 caagttgatc gcgagtaaaa tgcgcggtat caaccattgc cgttgcgcgg ctcaaatttt
     3541 cagtaatttg tgggaaagac atacaaacaa ccacaccagc acttaaaatt gtcacgcata
     3601 agcaaataat cgcacaaatt gttattaata cttgtttcat aaacttgtag tataatgcaa
     3661 cacctaacaa attgacctaa ttttgggaga taagatggcg gaaacttcaa attatttgtt
     3721 tacttcagaa agcgttaccg aaggtcatcc agacaaaatt tgcgatcaaa tttctgacgc
     3781 aattttggat gaactattgg cgcgcgagaa gcagcttgag cttcgtaatt ttgaagatga
     3841 gcatggtaaa gcaaatgtta agcgagtccg ttgtgcttgc gaaactcttg tctcaaccgg
     3901 tcttgtaatg ctaactggcg aaatccgcac tcaggcttat gttgacattc ccagaattgc
     3961 tcgtgatgtc attcgtgata ttggctacac tcacactgaa tatggttttg acgcagaaac
     4021 ttgcagcgtt caaaatactg tccatgaaca aagtccagat attgcaatgg gtgtcgatga
     4081 atctgtcgaa gcgaaggcaa aaagcactga taaatatgac ctaattggtg caggtgatca
     4141 gggtattatg tttggctacg catgcaacga aactaagact ctaatgccta tgccaattta
     4201 tctagcacat aggctatccg aacgcctcgc tactgttcgc aaagacgaca tcattcccta
     4261 tcttcgtgct gatggaaaaa ctcaagttac aattgaatat aaagatggca aacctgtttt
     4321 agtaaataat gttgttcttt cgactcagca tgatgaggac ataaaagaat ttgaactcaa
     4381 gcgtgatgtt atggataagg tgattaaacc tgttcttgac gaagctggca ttccgactga
     4441 taaatcaatc gaatattaca tcaacccaac tggaaggttt gttcttggag gtcctgctgg
     4501 agacagcggg ctcaccggaa gaaaaattat tgttgataca tatggagggg ctggacatca
     4561 cggaggcggt tgctttagcg gaaaagattg cacgaaggtt gaccgctctg cttcgtatat
     4621 ggcaagatat gttgcgaaaa acattgttgc tgctggactt gcagacaaat gtgaaattca
     4681 aattgcttat gcaattggcg tagctcatcc aacttcaatt tttattgact gctatgacac
     4741 aaacaaagag ccaattgaaa aaattgaata tgcggtttct caaacttttg atctccgacc
     4801 tgctgcaatt caagatgctc ttgacttatg gcgtccaatt tatcgcaaaa cttcaaatta
     4861 tggacacttt ggccgcgaac ttcctgaatt cacttgagaa aaaacggata aagcagatga
     4921 acttcaaaga cttttataat tttataaaaa ataaagcacc cttttcgtat atattagcaa
     4981 tattaattct tgctatttta attattatta cagttttcgt agttaaagag tatcgaaact
     5041 tcatgaacaa tattcaaata actataagcg cagaagaaga acttgccaaa ctggaagcag
     5101 aagaacaagc taaacgagat gaagaattaa aaaaacaatg agttgcacta aaagaaatag
     5161 aagttaccaa cacattaaag tctcttgggc tttctcgtaa aaagcttgac gtttcaataa
     5221 tataccaaca tcctgaattt ccagcaggat gtgaatgcat cgcccttaca aacatgatga
     5281 actattataa attcaactta gaaaaatcca ctttaataga aaattatctt gagtatagcg
     5341 aatctgattt tgttaattat tatgttggat caccgtatac ttccggtatg ggtggcgtga
     5401 tgatgtgtcc tgcaataagc aatctgttca ataaatatat aacagtaaat ccagggccat
     5461 ttaaaggttt tgacataaca aaacgtaatt ttgaggattt atttgcatat atagaaagag
     5521 gacatcccgt tcaaatctga tgctctttag gaatgggagg tcttggtgag gtattatctt
     5581 catttaataa ttatttacta tatgaaaaca ctcattcagt attactaact ggttttgata
     5641 gagaaaatgg aattgtttat attgctgatt cgattaacgg ggatgtttat tacaacattg
     5701 agacagtccg caacatattt gaacaacaaa attcgcaagc attcgtaatg ataagccagg
     5761 aagaacttga taaatgaatt tgtggtcaag attgatctta cgacgatact atcccaaaaa
     5821 atacagatga taattctgac gaagaagagt ctaacgaaga ttaattctac atgtagtcta
     5881 aaccgttaac tggtgtgcca tta
//
LOCUS       DBNCDC010000071         5785 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_7989_length_5785_cov_5.048342, whole genome shotgun sequence.
ACCESSION   DBNCDC010000071 DBNCDC010000000
VERSION     DBNCDC010000071.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5785)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7989_length_5785_cov_5.048342"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(195..338)
                     /locus_tag="AC04VQ_05640"
                     /pseudo
     CDS             complement(195..338)
                     /locus_tag="AC04VQ_05640"
                     /inference="COORDINATES: protein motif:HMM:NF042587.5"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
     gene            complement(503..1354)
                     /locus_tag="AC04VQ_05645"
                     /pseudo
     CDS             complement(503..1354)
                     /locus_tag="AC04VQ_05645"
                     /inference="COORDINATES: protein motif:HMM:NF022077.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
     gene            1453..2172
                     /gene="nagB"
                     /locus_tag="AC04VQ_05650"
     CDS             1453..2172
                     /gene="nagB"
                     /locus_tag="AC04VQ_05650"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015760985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HIO9553613.1"
                     /translation="MKVIVDSRQNIAEAAAQGYVSFIQNKPNAVLGLATGSTPEDLYA
                     ELIKLCNQGEVTFKDVTTFNLDEYCGLTPDHPQSYRYFMQDKLFDHIDINPRNTFVPD
                     GFRTSEEDLIAYDRAIAEHGGIDLQLLGIGGNGHIAFNEPGTSFQSITHMVELTPETR
                     QANSRFFNSIDEVPTHAICMGMRTIMNTQNIILMAIGKNKAEAVRNTVYGNIDPMCPA
                     SVLQLHPNVTIFVDEEAGSLL"
     gene            2188..3540
                     /gene="glmM"
                     /locus_tag="AC04VQ_05655"
     CDS             2188..3540
                     /gene="glmM"
                     /locus_tag="AC04VQ_05655"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIO9553614.1"
                     /translation="MGKYFGTDGFRGKANIDLNSNHAFLIGSFFGQLAAKEGTKEKKI
                     VIGQDTRQSGDMFVNALAAGITSSGADAYILGTVPTPGVAFVTKSYGFNYGIMVSASH
                     NPYEDNGIKVLNNNGEKLNEDIVLDCEDYIDAGIDNVVLAQSADIGESSGFHSAHIRY
                     IDHLKSCVSHSFDGLKIALDCANGSASNFAGEIFRDLGADVHVLHNDPNGLNINVDCG
                     STHIESLCKYVKDNGYDMGFAYDGDADRCLAADKNGKEVSGDELMLLLARRLKNEKKL
                     NKNVLVTTIMSNFGLYKALDELGIGYEKTAVGDKYVYENMLENKHSLGGEQSGHIIFS
                     SLATTGDGMLTSLMVVDSLIELGETLDEAASKMTVYPQVLKNVTVDDKEKTLLDDVVI
                     ASIDKAEERLKGDGRVLVRPSGTEPYVRVMAEASTYELCEKAVDDIISAISKSGHLKK
                     "
     gene            3550..4215
                     /locus_tag="AC04VQ_05660"
     CDS             3550..4215
                     /locus_tag="AC04VQ_05660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF051307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIO9553615.1"
                     /translation="MDAKIEQLLDLQQTIAAPLFEGKTYPWEVLPEIKDFILKLGPTL
                     DTSKFDHPSEDVWIAKSANVAESASIAGPLIIDEEAEIRPCAYIRGNAIIGRGATLGN
                     SCEIKNSIMFNESQTPHFNYVGDSIIGYKSHIGAGGITSNLKSDKSNVVVKSVDGENI
                     ETGLRKFGAILGDNVEVGCNSVLNPGTVLGRNSMVYPTSGVRGFIPENSIFKAPDNIV
                     MKK"
     gene            4215..4772
                     /gene="acpS"
                     /locus_tag="AC04VQ_05665"
     CDS             4215..4772
                     /gene="acpS"
                     /locus_tag="AC04VQ_05665"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIO9553616.1"
                     /translation="MSTNEVRNTNDSKTVGLGVDIVDVTRMRDILNKTPNFIEHTYTS
                     QEAEYCQKSSRFVEHYATHFAAKEAVLKALGCGFSEGVSPKDVEVLHDDKGKPYVELH
                     GRVKGIAEELKIKDLPISLSFTNTEAVGFAIALTEESAFENEVLKKSKDPMAELTKQF
                     KEAKKILEEAPKELPNDPSNNEDQG"
     gene            4741..5619
                     /locus_tag="AC04VQ_05670"
     CDS             4741..5619
                     /locus_tag="AC04VQ_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIO9553617.1"
                     /translation="MIQVIMKIKDKKYSKKDLKNLLPFPDCDSNKYSRGTCNIVAGSE
                     SYPGASTLASLSASKAGSGYTHLFTHKSAKPIALAIMPSLVVSSFEDLSVEKFDTEKP
                     SAYLVGPGFSGEASEKKILLKILKETDAPVVVDGGALSAFSSEEVRITVEERFNNGLD
                     TILTPHMGEARALLFNYAVKKFTSSEELAKDLAYFTKSTIVLKGPNTFVCDEEHIYEM
                     KSGGPALAKAGSGDVLAGILGGIVCQNRLSAFNACVLATNIHAQAGNLAAKDLTEISV
                     TSEDLINYIPKSIKYF"
BASE COUNT         1790 a         1091 c         1155 g         1749 t
ORIGIN      
        1 tctaaaattt tcgtgccgcc cttaatagca tgaattgtgc ctggattgat ttggaaaaaa
       61 tctcctggat gaatttcaaa ttcgtttaaa acatcatccc atctattctc gtcaatgcat
      121 tctttcatgc tttcacggtc tttagattta tgaccaataa ttattgttgt tccaggctca
      181 caatcaagaa catatcaaca ttctttttta ccataaggtt cgccttcgac tttttgagca
      241 taagcatcgt caggatgaac ttgaacgctc aaatctttat tcgcttcaat aaatttaaca
      301 aggtgaggga actcagccat ttccagattc ccaaaaagct cacgatgtga ctctcaaagt
      361 tcacgtaaat ttttaccttt aaagttccca tttttgataa tgctcgacgc atctttatat
      421 gcagaaatac ctcaagattc gcttcctcaa attaaaggaa cgaagtgagg ctccataaat
      481 agtatttcgt tattagcatt cattaaagag aggtaataac cttcgccgtt ttttcaagta
      541 gcttatctgt gttgtattcg gagatctttc cttcgtcaca taatgcagca aattcaggat
      601 caattggaag tttatcaata tctttaatat cgtatttttt gccaatttct tcagctctgc
      661 tttttccaaa gatctcatgt tttttagagc agtccggaca tttgtaatat gacatgtttt
      721 ccaccaagga aagcactttg acgttcatca ttgctgctaa gtttattgct tttccaacaa
      781 tcatttctac caaatcttga ggagtagaaa cacaaattat gccgtcaaca ggaagtagtt
      841 gcataacggt taaaaacaca tccgaagttc caggaggcat gtcaaccaat aaaacatcta
      901 tatctcctca atttgtcatg ttaaagaatt gagaaattgc gccagaaacc acagggcctc
      961 ttcacgccac tggactatca gggtcttcaa gcagcaaatt tgcagaaatc atcttaatac
     1021 cgtctttact tacagctggg ttcatagctt cttcggtagc gcttaaatct ccagagactc
     1081 cgaagacctt cggtacactt ggaccagtaa catctgcatc taagattcca acttttaatc
     1141 cacttttatt tagtttgctt gctaaaaggg aacataccaa tgactttcca acgccacctt
     1201 ttccagaaac tacaccaatt acatgcttga tatttgaatt tttattaggg ctaagtttct
     1261 ctggcgtttg atgttcgcaa tttccagcct tggcacatgt cgaacaatca tgctcgcaag
     1321 actcacaatt ttcatgatcg catttatcgc tcataacatc tcctttttta agtaggcagt
     1381 ataatagcac aatttaatga tataattgcg atagcaaatg cagaataaac gaagttatta
     1441 ttggaggtaa taatgaaagt aattgtcgat tcaagacaaa acatcgccga agccgcagca
     1501 caaggctatg ttagtttcat ccaaaacaaa ccaaatgcgg ttttgggttt agcaacaggc
     1561 tcaactcctg aagatttata cgctgagctc ataaagttat gcaatcaagg cgaggtaact
     1621 tttaaagatg taaccacatt taaccttgat gaatattgtg gacttactcc tgatcatcct
     1681 caatcttata gatattttat gcaggataag ttgtttgatc atatcgatat taatcctcga
     1741 aacacttttg ttcctgatgg ttttagaaca tctgaagaag accttattgc ttatgatagg
     1801 gctattgctg aacatggcgg aatcgatctt caacttttag gcattggcgg taatggccat
     1861 attgcattta acgaacccgg aacttcgttt caaagcatta ctcatatggt tgagctaacg
     1921 ccggaaactc gtcaagcaaa ttctagattt tttaatagta ttgacgaagt tccaactcat
     1981 gcaatttgta tgggtatgcg tacaattatg aatactcaaa acattattct tatggctatt
     2041 ggcaagaata aagccgaagc tgttcgaaat actgtttatg gcaatattga tccaatgtgt
     2101 cctgcttctg tgcttcaatt acatccaaat gtaacaattt ttgtcgacga agaggctgga
     2161 agtttacttt aatctaaggt ttttattatg ggtaagtatt ttggtaccga tgggtttagg
     2221 ggtaaagcta atattgacct aaactccaat catgcatttt taattggatc tttctttggt
     2281 cagcttgctg caaaggaagg cacgaaagaa aagaaaattg taattggtca agacacgcgt
     2341 caaagtggcg atatgtttgt aaacgctttg gctgccggta ttacttcgtc tggagcagat
     2401 gcctacattt taggaacagt tccaactcca ggcgtggcct tcgtaacaaa atcatatggt
     2461 tttaattacg gaattatggt ttctgcaagt cataatccat atgaagacaa cggcattaag
     2521 gtgttaaata acaacggtga aaaacttaac gaagacatag ttcttgattg cgaagattat
     2581 atcgacgctg gtattgataa tgttgttttg gctcaaagtg ccgacattgg ggaatcaagc
     2641 ggatttcaca gtgcacacat tcgatacatt gaccatttaa aatcttgcgt gtctcattct
     2701 tttgatggcc ttaaaattgc tcttgattgc gctaatggct ctgcgagcaa ttttgctggg
     2761 gaaattttta gagatcttgg ggcagatgtt catgttcttc acaatgatcc taacggctta
     2821 aatatcaatg tcgattgtgg ctcaacacac attgagtcac tttgcaaata tgtaaaggat
     2881 aatggttatg acatgggctt tgcctatgat ggtgacgctg ataggtgcct cgctgcagac
     2941 aaaaacggga aagaagtttc tggcgatgaa cttatgttgc tgttagctcg tcgtctcaaa
     3001 aacgaaaaga agcttaacaa aaacgtttta gttacaacaa ttatgagcaa cttcggactt
     3061 tataaggcac ttgatgagct tgggattgga tatgaaaaga ctgccgttgg agacaaatat
     3121 gtttatgaaa atatgttaga gaacaagcat tctcttggcg gtgagcaatc tggtcatatt
     3181 atttttagca gcttggcaac aactggggac gggatgctta caagcttaat ggtcgttgac
     3241 tctcttattg aacttggcga aactcttgac gaagctgctt ctaagatgac cgtatatcct
     3301 caagtcctaa agaatgttac tgttgacgac aaagaaaaaa ctttgcttga tgatgttgtt
     3361 attgcttcta tcgataaagc tgaagaacgt cttaaaggcg atggcagagt tcttgttcgt
     3421 ccaagcggaa ctgaacctta tgttagagtt atggctgaag caagcacata tgagctatgc
     3481 gaaaaagccg tcgatgatat tatttcagca atttcaaaaa gcggtcattt aaaaaaatag
     3541 gagagagttg tggatgctaa aatagaacaa cttttagact tacagcaaac tatagccgcc
     3601 cctttatttg agggcaaaac ctatccgtgg gaagtgctcc cggaaataaa agattttatt
     3661 ttgaagttag gcccaactct tgacacttcc aaatttgatc atccctcaga agatgtttgg
     3721 attgctaagt cagctaacgt cgcagaaagc gcttcgatag ctggcccttt aattattgac
     3781 gaagaagctg aaatccgtcc ttgtgcctat attcgcggaa atgcaataat tggtcgaggt
     3841 gcaactcttg gtaattcttg tgaaattaaa aactctataa tgtttaacga atcacaaact
     3901 ccacacttta attatgttgg agattctatc attggttata aatcacacat tggtgccggc
     3961 ggaatcactt cgaatcttaa aagcgataaa tccaatgttg ttgttaagtc tgtggatggc
     4021 gaaaacattg aaactggact tcgcaaattt ggtgcaatat taggcgataa tgtcgaagtt
     4081 ggatgcaaca gcgttcttaa tccaggaacg gtgcttggca ggaattcgat ggtttatcca
     4141 acaagtggtg ttcgtggatt cattcctgaa aattcaattt ttaaggctcc tgacaatatt
     4201 gtgatgaaga aataatgtct actaacgaag ttcgaaatac taatgattct aaaactgtag
     4261 gcttaggcgt tgacattgtt gatgttacac gaatgcgtga tatcttaaac aaaaccccaa
     4321 acttcataga gcatacatat acttcgcaag aagccgaata ctgtcaaaaa tcatctaggt
     4381 ttgtagaaca ttatgcaact cattttgctg ctaaggaagc ggtacttaaa gctttaggct
     4441 gcggttttag cgaaggagtt agcccaaaag acgttgaagt tttgcacgat gacaaaggca
     4501 agccttatgt cgagttacat ggtagagtca agggtattgc agaagaactt aaaattaagg
     4561 atcttccaat ttctttgtcg ttcacaaata ctgaagcagt tggcttcgcg attgccttaa
     4621 cagaagagtc tgcgttcgaa aacgaagtcc ttaagaagtc aaaagaccca atggccgaat
     4681 tgaccaagca gtttaaggaa gctaagaaaa ttttggaaga agctccgaag gagcttccga
     4741 atgatccaag taataatgaa gatcaaggat aaaaaatact caaagaaaga tttaaaaaat
     4801 cttctcccgt tcccagattg cgacagcaat aaatattcac gaggcacttg caatatagtc
     4861 gctggtagcg aatcgtatcc tggtgcttcg acgcttgcgt cgctttctgc tagtaaggca
     4921 gggagtggat atactcattt gtttacacac aaatcagcta agccaatagc attagctatt
     4981 atgccaagtt tggttgtttc ttcttttgaa gacctttccg tagaaaaatt tgacacagaa
     5041 aagccctcgg cttatcttgt agggcccggc ttttctggcg aagccagcga aaagaagatt
     5101 ttacttaaaa tattaaaaga gacagatgct cccgttgttg ttgacggcgg tgctttaagc
     5161 gctttctctt ctgaagaagt aagaattact gttgaggaac gctttaacaa cggcctcgat
     5221 acaattttaa ctccacacat gggcgaggcc agagctttgc tctttaatta cgcagtcaag
     5281 aaatttactt cgtcagaaga actggcaaaa gatttagcct acttcacaaa gtccaccatc
     5341 gttcttaaag gcccaaatac atttgtgtgc gatgaagagc atatttatga aatgaaatct
     5401 ggcggcccag cattagcgaa agccggcagc ggtgacgttt tggcaggcat tctcggtggt
     5461 attgtgtgcc aaaaccgctt aagcgcattt aatgcttgcg ttctcgcaac aaacatacat
     5521 gcgcaagctg gaaatttagc agctaaagac ttaaccgaaa ttagcgtaac atcagaagat
     5581 ctaataaact acatcccaaa atctataaaa tatttttaat aaaattagtg caattttacc
     5641 tggaatttgc tacaatatcg cttgcgttat tttagatttt atgaggtgag aataatggca
     5701 gtacctaagc aaagaaaagg tcgaagcgct acaagaactc gtagaagcgc taacgataaa
     5761 gtaaaagtag caggtaaatc aacat
//
LOCUS       DBNCDC010000072         5761 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_8061_length_5761_cov_8.110761, whole genome shotgun sequence.
ACCESSION   DBNCDC010000072 DBNCDC010000000
VERSION     DBNCDC010000072.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8061_length_5761_cov_8.110761"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="AC04VQ_05675"
     CDS             complement(<1..475)
                     /locus_tag="AC04VQ_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIO9553618.1"
                     /translation="MATNSKNKKSGEDVVVKDIYSRVRVASADTIEDSRTNIGKHVAQ
                     KITDPDERFQDLKKACDYLSDKDMKTLAKAYEFAKESHKDQKRKSGEMFVVHPIEVAI
                     ILADLHMDIDTLCAALLHDTIEDTDANAKELNKLFGYDVALLVEGVTKITQIEVGS"
     gene            complement(482..631)
                     /locus_tag="AC04VQ_05680"
     CDS             complement(482..631)
                     /locus_tag="AC04VQ_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553619.1"
                     /translation="MKDEVKNIPENVLRQEYKKFQEAEEDMSIAEMLHDNEVLAGGKI
                     VGSDL"
     gene            complement(638..2053)
                     /locus_tag="AC04VQ_05685"
     CDS             complement(638..2053)
                     /locus_tag="AC04VQ_05685"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006531 - aspartate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HIO9553620.1"
                     /translation="MAKKNTEEFRIEHDFLGDKEVPIDAYYGVQTMRAIENFPITGYT
                     IDRALIIAMGIVKKAAAHANMDIGLLTEKKGKAIMDAATEVIEHTNLHEWFVVDPIQG
                     GAGTSINMNTNEVIANRALEILGEEKGRYDIISPNSHVNMSQSTNDAFPCGIHIASII
                     RLDMLVDTLEKLIDSFETKAEEFGDSIKMGRTHLQDAVPIRFAQEFEAYATVLKRDLA
                     RIKNVKHNLCGVNMGATATGTGLNASPQYIKLAGEYLARYSKKPIYVMDNLVDATQNT
                     DAYTECSAALKVCMLNMSKICNDLRLMASGPRCGLNEINLPPRQPGSSIMPGKVNPVM
                     CEVVNQTAFQVVGNDMTICLASEAGQFELNVMEPVLAFNLIQSINVTRNVLHTFRINC
                     VDGITINADRGQEFVDKSVGVITAINPHIGYETAAQIAKEAITTGKPVRDIVLEKGVL
                     TKEQIDIILNPQNMTTPGISG"
     gene            complement(2132..3490)
                     /locus_tag="AC04VQ_05690"
                     /pseudo
     CDS             complement(2132..3490)
                     /locus_tag="AC04VQ_05690"
                     /inference="COORDINATES: protein motif:HMM:NF015562.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter family
                     protein"
     gene            complement(3648..4868)
                     /gene="tyrS"
                     /locus_tag="AC04VQ_05695"
     CDS             complement(3648..4868)
                     /gene="tyrS"
                     /locus_tag="AC04VQ_05695"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIO9553621.1"
                     /translation="MAKLSAEEQMKIISSGAAGIVPEEAMLKKLKKGKALNIKLGVDP
                     TAPDIHLGHAVPLRKLAQFQELGHKVILIIGDGTALIGDPSGRNSTRPQLTQDQIKDN
                     AKTYIDQAFKVLDKSKTEIRYNSEWLLKIDMKKLLELTSNFTVARILERDDFSNRYKN
                     NQPIALHEFLYPVMQAYDSVMIEADVELGGTDQLFNLLAGRELMEKMGMEPQVCLTLP
                     LLEGTDGVQKMSKSYGNYVGVNEAAADIFGKIMSIPDDLMPKYFRLASSVPVDEIEKI
                     EAGLANGSLHPNEIKRQLARNIAEIYHDAKAAAEAEERFDKIHKDKDIPDDIPEFKAD
                     LTPNDEGKVYLAKLLVDAKMAQSAGEARRLIDGGGVKINQEALPAKSYNVEPELLKDA
                     TIQVGKRKFVKILS"
     gene            4996..5709
                     /locus_tag="AC04VQ_05700"
     CDS             4996..5709
                     /locus_tag="AC04VQ_05700"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HIO9553622.1"
                     /translation="MNEAGDGKVVFFGGAGVSTESGIPDFRSQDGLYNQQYKYPPEMI
                     ISHSFFIQNPKEFYRFYFDRMIPDDSIKPNPAHLKLAELEKIGKLNGVVTQNIDGLHQ
                     AAGSEKVYELHGSILRNFCMTCHEPYDLPALKDARDEEGVPKCPKCGGMLKPDVVLYE
                     EPLDSSVMHQAIAAITQADLMIIAGTSLVVYPAAGLVDYFKGKHIAIINRDPTKQDQK
                     ADLCISQNVGEVFSQLNFK"
BASE COUNT         1768 a         1274 c         1044 g         1675 t
ORIGIN      
        1 ccgaaccaac ttcaatttga gtaatttttg taacgccttc aacaagaagc gcaacgtcgt
       61 aaccaaacag tttgttaagt tctttagcgt tggcatccgt gtcttcgata gtatcatgca
      121 aaagcgctgc acacaaagtg tcaatatcca tatgaaggtc agctaaaatt attgcaacct
      181 caatgggatg cacaacaaac atttcaccgg attttcgctt ttgatcttta tgagattcct
      241 tggcaaattc ataagcctta gccaaagtct tcatatcttt gtcgcttaaa taatcacaag
      301 cttttttcaa atcctgaaag cgttcatctg gatcagtaat tttttgcgct acatgcttgc
      361 caatattcgt tcggctgtct tcgattgtgt ctgcagaagc aactctgaca cgactataaa
      421 tatcttttac gactacgtct tcaccactct ttttattttt cgaattcgta gccatttttc
      481 attacaaatc agatccaaca atctttccgc cggccaaaac ttcgttgtca tgaagcattt
      541 ctgcaattga catatcttct tcagcttctt ggaacttttt atattcttga cgaagaacat
      601 tttcaggtat attttttact tcgtccttca taagttctca acccgaaatt cctggagttg
      661 tcatgttttg aggatttagg ataatatcta tttgctcttt ggtcaaaaca cctttttcta
      721 aaacaatatc acgaactggt ttgccagtgg taattgcttc ctttgcaatt tgagcggcag
      781 tttcataacc aatgtgcgga ttaattgcag taataacacc aacactcttg tcgacaaatt
      841 cttgacctct gtcagcattg attgtaatac cgtcaacaca attaattcta aatgtatgca
      901 aaacattacg tgtaacatta attgattgaa tcaaattaaa cgccaaaaca ggttccatga
      961 cattaagctc aaactggccc gcttcgcttg ccaaacaaat tgtcatatca ttaccaacaa
     1021 cttggaaagc ggtttggtta acaacctcgc acataacagg gttaacttta cctggcataa
     1081 tcgaagaacc aggctgacga ggcggaagat taatctcgtt taaaccacaa cgaggaccag
     1141 atgccatcaa acgaagatca ttacaaatct ttgacatgtt aagcatgcaa accttaagcg
     1201 ctgcagaaca ttcagtgtaa gcatctgtgt tttgagttgc atcaaccaaa ttatccataa
     1261 cataaattgg cttctttgaa tagcgagcaa gatattcgcc agctaattta atatattgag
     1321 gagatgcgtt aagaccggtt cctgtagctg ttgctcccat attaactccg cacaaattgt
     1381 gctttacgtt tttaatacgg gcaagatcgc gcttaagaac tgttgcataa gcttcgaatt
     1441 cttgagcaaa acgaatcggc acagcgtcct gtaagtgagt acgtcccatc ttaatgctgt
     1501 caccaaattc ttcagccttt gtctcaaagg aatcgatcaa tttttcaagt gtatcaacca
     1561 acatatcaag acgtataata gatgcaatat gaattccgca agggaaagca tcatttgttg
     1621 actgagacat atttacatga ctgttaggtg aaataatgtc atatcttcct ttttcttctc
     1681 ccaaaatttc caaggcacga ttagcaatta cttcgttagt attcatattt atcgaagttc
     1741 cagcaccacc ctggattgga tcaacaacaa accactcatg tagatttgtg tgttcaataa
     1801 cttctgttgc agcgtccata atggccttgc ccttcttttc agttaaaagg ccaatatcca
     1861 tatttgcatg agccgcagct tttttaacaa tacccattgc aataattaaa gcacggtcaa
     1921 ttgtataccc agtaattgga aagttttcaa ttgcgcgcat tgtttgaacg ccataatatg
     1981 cgtcaatagg aacttccttg tctcctagaa agtcatgttc aattcgaaat tcttcagtgt
     2041 tcttcttagc catttactcc tcctaataat cgtaaaaatg aaacacccaa tgcactgact
     2101 aaatgcattg gatgtttatg tcccaaaata tctaaaagcc aataactaca gatgctaatc
     2161 ctattccaaa tgcaacggaa agcactgtta ccaccattcc tggcaacata aagctgtggt
     2221 ttagtacgta tttaccaata tgcgttgttc cagttcgatc aaagttgatt gcagcaacac
     2281 atgttggata gtttggaata aagaagtaac cattaactgc tgggaacata ccaacaagca
     2341 atggtgcagg aattccaagt gatacaccca aaggcattaa ggtacgaact gttgcagcct
     2401 ggctaaataa caaaattgaa agagcgaaaa gagcaattgc aaataaccat ggcatttgcg
     2461 aaacaatatc agcaatgctg tttgtgatga attctttgtt tgcttggaag aatgtatcac
     2521 ccatccatgc aacaccaaaa attgcaacaa cagcaccagt accagcttcc caaacagaac
     2581 ctttagggat tttagcaatt gattcttttc cttctttttt catcatatat aaaatgataa
     2641 ttgcagcaca caaaagcatt aacatttcaa ctgccgcaat catagaaaga taagttgtgt
     2701 ttccagttgc atctgtccat gaaggacgaa ttgcatcaac aacaccaacc aacaaaatta
     2761 cagcaaagaa aactaaaaag ataataacac ttccctttgc gcaagcagat ggcttaaagt
     2821 tgtcaacttc ttttgagtca ctaggtggct caaccaaacc ttgttttaag cgatcctgat
     2881 attctggatc atcatcaagt tctttaccaa tcttactttg aataaatgca ccaatcatgc
     2941 aagcaacaaa tgttgcaggg aaagaacaag ctatgatctg gattaatgaa acatcagcac
     3001 cagataaaat agagagaagc gaagcagtag cagcagcaat tgggcttgca gttacagctt
     3061 gctgtgatgc aataacagca attgacattg ggcgttcagg acgaattcca gcgcctcttg
     3121 caatttctgc gatagcaggc aaaatagcat atgagcagtg acctgtacct tcgcaaaatg
     3181 taaagaaata agtgacgata ggagcaagga atgtgattcg cttcggattt tttcgaagta
     3241 gcttttcact cttgttaaca attcaatcca tgcccccggc agcttgcagc gttgccgccg
     3301 cagtaacaac tgccaaaatg ataagcataa cgtcaaccgg tggattttga ggcatagctc
     3361 caaagccaaa taccaggata accataccaa gaccacccat aacgccgagt cccatgccgc
     3421 caactcttgc accaataaaa atacaagcaa gaacaatagc gaactgcacg acaaacatta
     3481 ttgcatccat tatttctcct ctcgagaatt tttcttattg tgacctgcca aaagatgctt
     3541 ttaagctacg tttaatagac atacgttcac acattgcgaa attataacaa agaccagcga
     3601 aattacaaga aaattttacg aaaatgctta aattttaatt aaaaatttta ggacaggatt
     3661 ttaacgaatt tgcgcttgcc aacttgaatt gttgcatcct tcaaaagttc aggctcaacg
     3721 ttatatgact tcgcaggtaa agcctcttga ttaatcttaa ctccaccacc atcaataaga
     3781 cgtctagctt cgccagcaga ttgggccatc ttcgcatcaa caagaagttt cgcgaggtag
     3841 accttgcctt cgtcattagg tgttaagtca gccttaaatt cagggatatc atcagggata
     3901 tccttatctt tgtgaatctt atcgaagcgc tcttcggctt cagctgcggc cttagcatcg
     3961 tgataaatct cagcgatgtt gcgcgcaagc tggcgcttta tctcatttgg atgaaggctg
     4021 ccattggcaa gtcctgcttc gattttttca atttcgtcaa ctggaacact tgaagccaat
     4081 cggaaatact tcggcattaa gtcatctgga atagacatga tttttccgaa gatatccgca
     4141 gccgcttcgt taacaccaac atagttgcca taagacttag acattttttg aacgccatct
     4201 gtgccttcga gaagtggaag cgtcaagcaa acttgcggct ccatgcccat tttttccata
     4261 agctcacggc cagcaagcaa attaaaaagc tgatcggttc cgccaagttc aacatcagcc
     4321 tcgatcataa cgctatcgta agcctgcatt acaggataca aaaattcgtg aagcgcaatt
     4381 ggctgattgt ttttatagcg gttgctaaaa tcatcgcgtt ccaaaattcg cgcaacagta
     4441 aaattcgaag tcaactccaa aagttttttc atatcaattt taagaagcca ctcgctgtta
     4501 taacgaatct cggttttgct tttatcaaga accttaaatg cctggtcaat atatgttttt
     4561 gcgttatcct taatttgatc ttgagtaagt tgagggcgcg tagaatttcg gcctgatggg
     4621 tcgccaatca aagcggtgcc atcgccaata atcaaaatta ctttgtgacc aagttcttgg
     4681 aattgagcaa gtttgcgaag aggaacggca tgtcccaaat ggatatcagg cgcagtcggg
     4741 tctactccaa gcttaatatt tagagcttta cccttcttga gttttttgag catcgcctct
     4801 tcgggaacaa tcccagcagc tccgctcgat ataattttca tctgttcttc tgcgcttaat
     4861 ttagccatta cgtgcctttc tctcgccgat ttattataga caattaagtt taaaatgccc
     4921 aatctttttc aatttgctat aattgaaatg ttgaaaggaa tcatcatgga tgaaatcaag
     4981 actctaaaca attgaattaa cgaagccgga gacggcaagg ttgttttctt tggcggagca
     5041 ggtgtttcga ccgaaagcgg aattcctgat tttcgaagcc aagacggact ttacaaccag
     5101 caatacaaat atccgccaga gatgattatc tctcacagtt ttttcattca aaatccaaaa
     5161 gaattttacc gtttctactt cgaccgtatg attccagatg acagcattaa gccaaatcca
     5221 gctcacctaa aacttgcaga gcttgaaaaa attggaaagc taaacggcgt tgtcacacaa
     5281 aatatcgatg gacttcacca ggctgctggt tccgaaaaag tttacgagct tcacggttca
     5341 attcttcgta acttttgtat gacgtgccac gaaccttacg atcttcccgc actaaaagat
     5401 gcgcgtgacg aagaaggggt ccccaagtgc ccgaagtgcg gtggaatgct taaacctgat
     5461 gttgtacttt acgaagaacc gctcgacagt tctgtaatgc atcaagcaat cgcagcaatt
     5521 acgcaagctg accttatgat tattgcaggt acttcgcttg tcgtttaccc tgctgcaggg
     5581 cttgtggatt acttcaaggg aaaacacatc gcaataatta accgcgaccc cacaaaacaa
     5641 gaccaaaaag ctgacctctg catttcacaa aacgtcggcg aagtcttctc ccaactaaac
     5701 tttaaataga aaaccccatt ttctttcgag gacccagttt ttaaactagg tgggtgtccg
     5761 c
//
LOCUS       DBNCDC010000073         5715 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_8170_length_5715_cov_8.998057, whole genome shotgun sequence.
ACCESSION   DBNCDC010000073 DBNCDC010000000
VERSION     DBNCDC010000073.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5715)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8170_length_5715_cov_8.998057"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(23..571)
                     /gene="rplE"
                     /locus_tag="AC04VQ_05705"
     CDS             complement(23..571)
                     /gene="rplE"
                     /locus_tag="AC04VQ_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015539826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIO9553623.1"
                     /translation="MAMPRLKEKYQKEIVPAIMKEFGYKNVNQVPRLEKVVVNMGVGD
                     AATDKKLINGAIDDLSVITGQKPTTTRAKKSIAGFKLREGMAIGAKSTLRGDRMWEFI
                     DRLLATALPRVRDFRGLSPKSFDGRGNYSLGLTEQLVFTEVDYDKVDHTRGMDITVVT
                     TANENDPAFKLLQMLGFPFAEK"
     gene            complement(585..902)
                     /gene="rplX"
                     /locus_tag="AC04VQ_05710"
     CDS             complement(585..902)
                     /gene="rplX"
                     /locus_tag="AC04VQ_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009607843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIO9553624.1"
                     /translation="MAKLQIKKGDTVRVLSGKDKGKEGEVIRAYPSTNRVIVEKVNMI
                     KRHMRPTQQNPNGSISEKEATIDASNVMLICPACNKPVRVGHVKDEKGNMQRVCKKCK
                     KTF"
     gene            complement(915..1274)
                     /gene="rplN"
                     /locus_tag="AC04VQ_05715"
     CDS             complement(915..1274)
                     /gene="rplN"
                     /locus_tag="AC04VQ_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIO9553625.1"
                     /translation="MQTYLSVADNSGARKVQCIKVLGGSKRRYAGLGDKIVCAVKEAI
                     PGGAVKKGDVVKCVVVRTKNQVRRNDGSYIRFDQNACVIINDEGEPRGTRVFGPVARE
                     LRDKKFTRIVSLAPETL"
     gene            complement(1302..1571)
                     /gene="rpsQ"
                     /locus_tag="AC04VQ_05720"
     CDS             complement(1302..1571)
                     /gene="rpsQ"
                     /locus_tag="AC04VQ_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIO9553626.1"
                     /translation="MAEKKETRNLRKTRQGVVVSASGEKTCVIKIEEKKQHPVYKKMM
                     TTSKKLHAHDEKNEAEVGDTVLVMETRPMSKLKHWRLLKIVEKAK"
     gene            complement(1583..1795)
                     /gene="rpmC"
                     /locus_tag="AC04VQ_05725"
     CDS             complement(1583..1795)
                     /gene="rpmC"
                     /locus_tag="AC04VQ_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012799448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIO9553627.1"
                     /translation="MADLKNAEIRTLSVEDLYARLKDARAELFNLRFQMATSQLDNTS
                     RVKVVKKQIARIQTEMRAREIAQSEA"
     gene            complement(1795..2220)
                     /gene="rplP"
                     /locus_tag="AC04VQ_05730"
     CDS             complement(1795..2220)
                     /gene="rplP"
                     /locus_tag="AC04VQ_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012799449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIO9553628.1"
                     /translation="MLMPKRVRHRKVQRGSMKGKAKGGTKLAHGSWGIRAMESAWITN
                     RQIEAARIAMTRAMKRGGKVWITIFPDKPITKKPLETRMGKGKGNPEGWVAVVKTNRI
                     MFEIDGVEEEVAKEALRLAITKLPIKCKIVTKETEEVLA"
     gene            complement(2220..2939)
                     /gene="rpsC"
                     /locus_tag="AC04VQ_05735"
     CDS             complement(2220..2939)
                     /gene="rpsC"
                     /locus_tag="AC04VQ_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015539832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIO9553629.1"
                     /translation="MGQKVHPTGFRLGITENWRSRWYADKDYADQLNNDLEVRKYLEK
                     RLANCALSKVEIERTGEKIKIIIVTARPGAVIGKKGADIEGLRTDVEKIVGAKVAIDV
                     VEVKRPELDATLIAKSVAEQLEGRIAFRRAMRKAVQSAQKSGAQGIRLQVSGRLGGAE
                     MCRREWYGEGRIPRHTLRAQIDYGFAIAATQMGSIGVQVWVYYGDVLPGQRPTNPALE
                     SSSRPAGRGRGKRPASERGAK"
     gene            complement(2939..3292)
                     /gene="rplV"
                     /locus_tag="AC04VQ_05740"
     CDS             complement(2939..3292)
                     /gene="rplV"
                     /locus_tag="AC04VQ_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIO9553630.1"
                     /translation="MESTAQAKYIRISPRKARMVVDQIRGKSVEDARLTLRFIERAAA
                     QDVESCLNSAVANAEDVHKLRAEQLKVTEAYVDEGPTLKRYRPRAKGAASRINKRTCH
                     ITVVVSNGKDDKKEA"
     gene            complement(3302..3571)
                     /gene="rpsS"
                     /locus_tag="AC04VQ_05745"
     CDS             complement(3302..3571)
                     /gene="rpsS"
                     /locus_tag="AC04VQ_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIO9553631.1"
                     /translation="MSRSLKKGPFVEPKLLSRIDAMNQAGKKEVIKTWSRSSTIFPDM
                     VGHTIAVHDGRKHVPVYISESMVGHKLGEFAPTRTFRGHSSSGKE"
     gene            complement(3672..4415)
                     /gene="rplB"
                     /locus_tag="AC04VQ_05750"
     CDS             complement(3672..4415)
                     /gene="rplB"
                     /locus_tag="AC04VQ_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIO9553632.1"
                     /translation="MKTFKPTSAGKRFQTVADFAEITKTTPEKSLIAKKKNHAGRNNY
                     GRITTRHQGGGVKRKYRVIDFKRDKDGIPAKVASIEYDPNRSSYIALLNYADGEKRYI
                     LQPKGLKVGDTVISGEGQDIKPGNAMKLDNIPVGSFIHAVEMQPGKGAAMARSAGTSI
                     QLMGKEGDYAILRMPSSEMRRVLLTCRATVGEVGNAEHVNIKIGKAGRKRWMGVRPTV
                     RGTAMNPVDHPHGGGEGKNKSAGRHPVSP"
     gene            complement(4589..4894)
                     /locus_tag="AC04VQ_05755"
     CDS             complement(4589..4894)
                     /locus_tag="AC04VQ_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553633.1"
                     /translation="MVNSKDYLENYHPKDVGENILKNKVLRKFNLDISTATVSLSKKL
                     RDQIILFLNSNGATYKQIVRLTGVSYGIVLRVVTDHFKQQNRVNATSKSSTGPGEIE"
     gene            complement(4985..5392)
                     /locus_tag="AC04VQ_05760"
     CDS             complement(4985..5392)
                     /locus_tag="AC04VQ_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIO9553634.1"
                     /translation="MSTKRIQNETGYYHITARGNGKQIIFDDDADYLRYLELLDKYNN
                     NFNIEILAYCLMSNHIHLLIYDPEFNMDKLFRQVHSMYTRSFNYKYGRTGNLFQGRFS
                     SKGIEDDKQLMAALRYVLLNPEEAGFCKACDYK"
BASE COUNT         1542 a         1350 c         1057 g         1766 t
ORIGIN      
        1 ggttttcctc tcttaccatc ttttatttct cggcgaatgg gaagcccaac atttgtagca
       61 atttaaaagc tgggtcattt tcattcgcag tagttacaac tgtaatatcc ataccacgtg
      121 tgtggtcaac tttatcgtaa tctacctcgg taaatactaa ttgctctgta agaccaaggc
      181 tgtagtttcc gcgaccgtcg aaactcttcg ggctaagacc acgaaagtct cgaacacgag
      241 gaagtgcagt cgctaacaaa cggtcaataa attcccacat acggtcccca cgaagagtgc
      301 tcttcgcacc aattgccatt ccttcgcgaa gcttaaaacc agcgatggat tttttagcac
      361 gagtagttgt aggtttttga cctgtgatta cgcttaagtc atcaattgca ccattgataa
      421 gttttttatc agttgcagca tctccaacac ccatgttgac aacaactttt tccaaacgag
      481 gaacctggtt aacattcttg taaccaaact ccttcataat tgctggcaca atttcttttt
      541 gatatttctc ttttaatcta ggcattgcca taatttatca cccactaaaa agtctttttg
      601 catttcttgc aaactctttg catattgcct ttttcatctt taacatgacc aacacgaaca
      661 ggcttattgc acgctggaca aataagcatt acgtttgaag catcaattgt ggcttccttt
      721 tcgctgattg atccgttagg attttgctga gtaggacgca tatgacgctt aatcatgtta
      781 actttttcta caattacacg gttagtagaa ggataagcgc gaattacttc gccttcttta
      841 cctttatcct taccgctaag gacgcgaact gtatcacctt ttttaatttg aagtttagcc
      901 atcacaatca ccgcctataa agtctctgga gcaagggaaa caatacgtgt aaatttctta
      961 tcacgaagtt ctcttgcaac cggtccaaac acacgagttc cgcgaggctc gccttcgtca
     1021 ttaattatta cacaagcatt ttggtcaaag cgaatgtagc tgccgtcatt tctgcgaacc
     1081 tgatttttag tgcgaacaac aacgcatttt acaacgtcgc ctttcttaac tgcaccacca
     1141 ggaattgctt ctttaacagc gcatacaatt ttgtctccca agcctgcata acgacgcttg
     1201 ctgccaccca aaaccttaat gcattgaact ttacgggcac cagaattgtc ggctacactt
     1261 aggtatgttt gcatctgtat catgataata cctgcctttt actatttagc cttctcaaca
     1321 attttcaaaa gacgccaatg cttcaactta gacattggac gagtttccat aaccaaaaca
     1381 gtatcgccaa cttcagcttc gtttttctca tcatgagcat gaagcttctt tgaagttgtc
     1441 atcatcttct tataaactgg atgctgtttt ttctcttcaa ttttaataac gcaagttttt
     1501 tcgcccgaag cacttacaac aacgccttgg cgagtcttac gtaaattacg agtttctttt
     1561 ttctctgcca tcatttactc ctttacgcct cactctgggc aatttctctt gcgcgcattt
     1621 ctgtctggat gcgagcaatt tgctttttaa caaccttaac acgcgaagtg ttgtccaact
     1681 ggcttgttgc catttgaaaa cgaaggttga aaagctccgc acgagcatcc tttaacctgg
     1741 catacaagtc ttcgacagaa agagttctaa tttctgcatt ctttaaatca gccattatgc
     1801 taatacctcc tctgtctctt tagtaacaat cttgcacttg ataggcagct tagtgattgc
     1861 aagacgaagt gcttccttgg caacttcttc ttcgacgccg tcaatttcaa acataattct
     1921 gtttgttttg acaacagcaa cccagccttc aggattacct ttacccttac ccatacgggt
     1981 ttcaagtggt tttttagtga ttggtttgtc tgggaaaatt gtgatccaaa ctttaccacc
     2041 acgtttcata gcacgagtca tcgcaatacg agcggcttca atctgcctgt ttgtaatcca
     2101 ggctgattcc atagcacgaa ttccccaaga accgtgagcg agctttgtgc cacccttggc
     2161 cttgcctttc atgctaccgc gttgtacctt gcggtgacgg actctttttg gcattaacat
     2221 tatttagcac ccctctctga tgccgggcgt ttgcctcggc ctcgaccagc tgggcgagag
     2281 ctgctttcaa gtgctgggtt ggtaggacgt tgtcctggaa gaacatcgcc ataataaacc
     2341 caaacttgaa cgccaatgct acccatttga gtagctgcaa tcgcaaaccc ataatctatc
     2401 tgagcacgaa gtgtgtgacg agggattcgg ccttcgccat accattcgcg tctgcacatt
     2461 tcagcgccac ccaaacgacc agatacctga aggcgaatgc cctgtgcacc tgacttttga
     2521 gcgctctgaa ctgcttttct catagcacgt ctaaatgcga tacgtccttc taactgttcg
     2581 gcaacacttt tagcaatcaa tgttgcgtca agttctgggc gcttaacttc cacaacatcg
     2641 atagcaactt ttgcaccaac aattttttca acatcagtgc gaagtccttc gatgtctgca
     2701 cccttcttac caataacagc accaggacga gctgtaacaa tgataatctt gattttttca
     2761 ccagtgcgtt cgatctcaac tttagaaaga gcacaatttg caaggcgctt ttctaaatac
     2821 ttacgaacct caagatcatt gtttaattga tcggcataat ctttatcggc ataccaacgg
     2881 ctgcgccagt tttcggtgat gcctaatcta aatccggttg ggtgaacttt ttgacccatt
     2941 atgcctcctt cttatcatct ttgccatttg atacaacaac tgtaatatgg catgttctct
     3001 tattaatgcg gcttgctgca cctttagcgc gaggcctata acgcttaagc gttgggcctt
     3061 cgtcaacata agcctcagtt actttaagtt gttcagcgcg aagcttgtgc acgtcttcgg
     3121 cgtttgcaac agcagaatta agacaagatt caacatcttg agctgctgca cgctcgataa
     3181 aacgaagcgt caggcgcgcg tcttcgacac ttttgcctcg aatttggtcc acaaccatgc
     3241 gggctttacg tggagaaatt cggatatact ttgcttgagc tgtactttcc atttatatca
     3301 cctattcctt acccgaagac gaatgaccgc ggaatgtacg agttggtgcg aattcgccaa
     3361 gtttgtgacc aaccatgctt tcgctgatat aaacaggaac atgcttgcgt ccatcatgaa
     3421 cagcaattgt gtgacctacc atatcaggaa aaattgtgct agaacgagac catgttttaa
     3481 taacctcttt tttgccagct tgattcatag catcgatgcg gctgagcaac ttaggttcta
     3541 caaaaggtcc tttttttaaa cttcttgaca tctaaaaatt cctttcatta tttagtgcgg
     3601 cggcgtctaa taattagacg gctgcttgcc ttcttaacat ttcttgtacg agcaccctta
     3661 gcaggttgtc ctcatggaga aactggatga cgaccagcac tcttgttttt accttcacca
     3721 ccaccgtgtg ggtgatcaac tgggttcatt gctgtgcctc gaactgtagg gcgaacaccc
     3781 atccagcgct tgcggccagc tttaccaatt ttgatattaa cgtgttcggc gtttccaact
     3841 tcgccaactg ttgcacggca agttaaaaga acacggcgca tttcagaaga tggcatacga
     3901 aggattgcgt aatcgccttc cttacccatt aactgaatgc tggttcctgc acttcgagcc
     3961 attgcagcac cttttccagg ttgcatttcc actgcgtgga tgaacgaacc aacaggaata
     4021 ttgtcaagtt tcattgcgtt tccaggcttg atgtcctgac cttcgccgct aataacggtg
     4081 tcgccaacct taaggccctt aggctgaagg atgtagcgct tttcgccatc agcataattt
     4141 agaagggcaa tgtagcttga acggtttgga tcgtattcaa tgcttgcaac cttagctgga
     4201 attccgtcct tgtctctttt gaaatcaata acgcgatatt tgcgcttaac tccaccacct
     4261 tgatgacgag ttgtgatacg gccataattg ttacggccgg cgtggttctt ctttttggca
     4321 ataaggcttt tttcaggggt tgtctttgtg atttctgcga aatcagcaac cgtctgaaaa
     4381 cgcttaccag cagaagttgg tttaaatgtt ttcattccca ctttaatctc ctttgtcttt
     4441 tgcgaggctt ctaacctgcg cattctttgc gccttatgca ggcgagtaca aatcttccac
     4501 gcttccgggc gtgttgattt tcccacgggc gcaagatggc attataccac tatttccccg
     4561 gggcccacgc tcgatttact cgtggcattt actcgatttc cccggggcca gtactcgatt
     4621 tactcgtggc atttactcga ttttgttgtt taaagtgatc tgtaacaact ctaagaacaa
     4681 ttccataaga aacacctgtt aaacgcacta tttgcttata tgttgcccca tttgaattta
     4741 aaaatagaat aatttgatca cggagttttt ttgacaatga caccgtggct gtggaaatgt
     4801 ctaagttaaa tttccttaaa actttattct taagaatatt ctccccaaca tcctttggat
     4861 gataattttc taaataatcc tttgaattaa ccaaagcttc aaattcatta aaactgacaa
     4921 gctggtcttc aatcctcttc gtattaattc tagaacatct atgcccatat aacttataac
     4981 tacttcactt gtaatcacat gccttgcaaa agcccgcctc ttccggattc aataaaacat
     5041 aacgaagcgc agccatcaat tgcttgtcat cctcaatacc tttactacta aatctccctt
     5101 gaaataaatt ccccgtccgc ccatacttat aattaaaaga ccgagtatac atcgaatgaa
     5161 cttggcgaaa taacttatcc atattaaatt ctggatcata tataagtaaa tggatgtgat
     5221 tgctcatcag gcagtacgcg agaatttcga tgttgaaatt attgttatat ttatctagca
     5281 attctaaata acgaagataa tctgcatcat catcaaaaat tatttgtttt ccatttcccc
     5341 tggcagttat atgataataa cctgtttcgt tctgaattct tttagttgac attttaattc
     5401 ctttctaaat cgagtactgg ccccggggaa atcgagtaaa tgccacgggc aaatcgagcg
     5461 tgggccccgg ggaaagtgac tatagcagaa aaggttcact ggaaaatgaa ggcgcaatca
     5521 ataaataata aagaatagat aaccacaaat aaacgagaaa ttattatttg aaaatatact
     5581 aaatataaat aaatataaaa tattactaat aaataaaatt aaagaaaaaa taaaaaatat
     5641 taaaatcgag taaatgccac gagtaaatcg agtactgacc ccggggaaat cgagtaaatg
     5701 ccacgagtaa atcga
//
LOCUS       DBNCDC010000074         5706 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_8188_length_5706_cov_7.818262, whole genome shotgun sequence.
ACCESSION   DBNCDC010000074 DBNCDC010000000
VERSION     DBNCDC010000074.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8188_length_5706_cov_7.818262"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="AC04VQ_05765"
     CDS             complement(<1..614)
                     /locus_tag="AC04VQ_05765"
                     /inference="COORDINATES: protein motif:HMM:NF017346.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HIO9553635.1"
                     /translation="MISCSGIGINDFIAIGQAKVVVTRTDEDYPVKGDDPKVELERLS
                     KAMETAKVQLQHLHDKTEETAGANEALIFEMHQMMLEDVEFSEMIEAEINGGSSAEHA
                     VILARDNFANMLASLDDDYMSQRADDVKDISARLLDCLTNTEAEAEESSDVPQIICAD
                     DLMPSQVVSMDLNSVHAIALAKGSTNSHASILANSFGLASVVNLG"
     gene            complement(640..897)
                     /locus_tag="AC04VQ_05770"
     CDS             complement(640..897)
                     /locus_tag="AC04VQ_05770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIO9553636.1"
                     /translation="MISFDYTIKDELGIHVRPAGQLAQLCKGKLSKSTIEIDGKEAEI
                     SKLFAVMALAVKCGDTITVKVDGADEEQTAEEVKKFLEENL"
     gene            complement(941..1423)
                     /locus_tag="AC04VQ_05775"
     CDS             complement(941..1423)
                     /locus_tag="AC04VQ_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIO9553637.1"
                     /translation="MGLFGKKKAEPCIVKAFEKGTVVAMKDVDDPTFAQEILGPGVAI
                     EPTEGVLYAPVSGTVSLVADTFHACSITCGCGAEILMHLGIDTVELKGEGFEAHVKAG
                     DKVSAGDKLITFDIDAIKGKGYKLTTPLIICNKDEFSAIDTIGSGEVAVGDDLLKLQK
                     "
     gene            1527..1602
                     /locus_tag="AC04VQ_05780"
     tRNA            1527..1602
                     /locus_tag="AC04VQ_05780"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1560..1562,aa:Thr,seq:cgt)
     gene            1649..2170
                     /locus_tag="AC04VQ_05785"
     CDS             1649..2170
                     /locus_tag="AC04VQ_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553638.1"
                     /translation="MKNFINIYVEHEALNYQLTKDILSQNSNANVITIGHYKDVFSRP
                     RQDVLLQKETQSLILAVKKDEFLYKGAPVCQDFGNQNFYYCSSVMNCVCNCEYCFLKG
                     MYESGNLVVFVNLENYFAQIKNDNKYICISYDTDLFPLESMLGYVKKWLDFARENNEV
                     TLEVRTKCKNLDV"
     gene            2195..2623
                     /locus_tag="AC04VQ_05790"
     CDS             2195..2623
                     /locus_tag="AC04VQ_05790"
                     /inference="COORDINATES: protein motif:HMM:NF045247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase family protein"
                     /protein_id="HIO9553639.1"
                     /translation="MFAFSLSPQKIISEFEHKTSNLDARLENIKAAQVRGFVTRLCFD
                     PIIYCQNWREEYSSLIEKTLEAIDISAVQDISIGSFRISQSYLKNMRARDRKSKIVNF
                     PFKNVGGYYQYDEALQTKMENFMLENLSKHFDKEKIFLWK"
     gene            2614..3351
                     /locus_tag="AC04VQ_05795"
     CDS             2614..3351
                     /locus_tag="AC04VQ_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553640.1"
                     /translation="MEIICYALDIEAMPLKKHFGENVRFEKCDVFAKNLDFLKTVSAN
                     DHITNIGICAGANVGEIYLCNKIHGEKTFYPDLLIPNNLEQREITTVDHVVNEDEVKA
                     HPNMLYDQEAAIIFEHAQKCIAPHQISFLKIVSDSGITDFEKIKKSIPQLIENKIPEI
                     EAFISSSQKFFSKIDTKEEIKNLDKYSELLKCSATMKVQLAQLLRYAQICNIDVENFF
                     KDIKISSKKDSLQILKSLKNFLLSEDK"
     gene            complement(3620..5011)
                     /locus_tag="AC04VQ_05800"
     CDS             complement(3620..5011)
                     /locus_tag="AC04VQ_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006362734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HIO9553641.1"
                     /translation="MTGIPFLIVFVIAIILMIVMISKFKVHPFLAILLVSLALGIIGG
                     IPLVDTVGADGEKVQGIANVIGAGFANTFTSIGIVIIFGAMIGSFLEATGAALTLADL
                     VVKLVGEKRPALAIELMGWIVSIPVFCDSGFVVLNPIRKALVKRTAASSVTMTLCLAA
                     GLYAAHVFIPPTPGPIAAANTLGVGNSILLVMGFGALVSIPALIAAYFYAKFIGKKVK
                     SADETSSDKEVVKTYEELKKSYGKLPNGWMSVAPILVPILLMAMSNISTILGWEGMLN
                     TCWQFLGTPIVALAVGVVLGIILLLTSKMGDKIYKLTEETLKTVGPILFVTAAGGVLG
                     KVIASTSLIEFITDNAAVLEPVGIFFPFIIAAILKSSQGSSTVAITTTAGLMAPVMAA
                     LGMGSEVMSVLCVMAIGAGAMTVSHANDSYFWVVTKFGDMTPEQGYKTQTVATLIMGV
                     VSIAFIFVLSLFF"
     gene            complement(5020..>5706)
                     /locus_tag="AC04VQ_05805"
     CDS             complement(5020..>5706)
                     /locus_tag="AC04VQ_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIO9553642.1"
                     /translation="LTDHLLDNEIRARYMHSDIGTLERVEILNDLRAGEFDVLVGINL
                     LREGLDLPEVTLVAILDADKEGFLRNHRSLIQTIGRAARNVSGEVIMYCEKITDSMKT
                     AIDETNRRRAIQVKFNEEHGIEPKTIRKAINSIMSFVSSDEVARSADEVNAELSDLSK
                     SEVLRIISSLEDDMAQASKDMDYEAAARLRDQVVKLRASIEGTSEDIILERLKQNTRK
                     GSKYGRRKRK"
BASE COUNT         1782 a         1235 c         1070 g         1619 t
ORIGIN      
        1 ccgaggttta ccaccgaagc taagccaaaa ctgttagcca aaatggaagc atgcgaattt
       61 gttgaaccct ttgcaagagc aatcgcatga acgctattta agtccatcga aacaacttgg
      121 cttggcatta aatcatccgc acaaataatt tgcggaacat cgcttgactc ttccgcttcg
      181 gcttcggtgt ttgttaagca gtccaaaagt cgagcagaaa tatctttaac atcatcggca
      241 cgctgagaca tgtaatcgtc atccaagctt gccaacatat ttgcaaaatt atcgcgagcc
      301 aaaatcacag catgttctgc cgaagaccca ccattgattt cagcttcaat catttcactg
      361 aactcaacat cctcaagcat catttgatgc atttcaaaaa ttaacgcttc atttgcgccg
      421 gcagtttctt cggttttatc gtgaagatgc tgaagctgaa ccttcgcggt ttccattgct
      481 ttagaaagac gctcaagttc aacttttgga tcatcgcctt taacaggata gtcctcatca
      541 gtgcgtgtga caactacctt cgcctgacca atcgcgataa aatcattaat tcctatgcca
      601 ctacatgaaa tcataattta gaaaattgtt tctccaaact tagagatttt cttcgaggaa
      661 tttcttaacc tcttcagcag tttgctcttc gtcagcacca tcaactttta ctgtaattgt
      721 gtcaccacat ttaacagcca atgccataac agcaaaaagc ttagaaattt cagcttcttt
      781 accatcaatt tcaatggttg acttgctcaa tttacctttg caaagttgag ctaattgacc
      841 tgctggtcta acgtgaattc caagttcatc tttaatcgta taatcaaaac ttatcattat
      901 ttctccttac attcacaaac gcgcgaaatt ataccataat ttatttttgg agttttaata
      961 aatcgtctcc aactgcaact tcgccacttc caatagtatc gatggcgcta aattcatcct
     1021 tgttgcaaat aattaaaggt gttgtgagtt tataaccctt gcctttaata gcatcaatat
     1081 caaaagtaat gagtttgtca ccagcagaaa ctttgtcgcc agccttgacg tgagcttcga
     1141 agccttcgcc cttaagctca acagtgtcaa ttccaaggtg catcaaaatt tcagcaccac
     1201 atccgcaagt aattgaacat gcatggaaag tgtcagcaac aagtgaaacc gttccgctaa
     1261 ctggagcata taaaacgcct tcagttggtt caattgcaac accaggtcct aaaatttctt
     1321 gagcaaatgt tggatcgtca acatctttca tagcaacaac agtgcctttt tcaaaagcct
     1381 taacaataca tggttcagct tttttctttc caaataaacc catagtttcc tcctttatac
     1441 ttaacctgat aattgtatca aaatttcacg aaatttcttt tattagttgc gattttgtcg
     1501 tgttttgtta tactttctcg ccacgagcca acgtagctca gttggtagag cgacgctctc
     1561 gtaaagcgta ggtcagcggt tcaatcccgc tcgttggctc caggaaaaat ttgccgctag
     1621 gcaaattttt caatactata actccactat gaaaaacttt atcaacatat atgtagaaca
     1681 tgaagcttta aattaccagc taacaaaaga tattctttct caaaactcta atgcaaacgt
     1741 aataacaatt gggcattata aagatgtgtt ttccagacct cggcaggatg ttttgctaca
     1801 aaaagaaacg cagagtttaa ttcttgcggt taagaaagac gaatttttgt ataaaggtgc
     1861 acccgtttgc caggacttcg gcaatcaaaa tttttactat tgttcgtctg tgatgaattg
     1921 cgtttgcaat tgtgaatatt gcttcttgaa gggaatgtat gagtccggaa atttagttgt
     1981 gtttgtaaat ttggaaaatt actttgccca aatcaaaaat gacaacaaat atatttgcat
     2041 ttcctatgac acggatctct ttcctcttga atcgatgctt ggatatgtta agaagtggct
     2101 tgattttgct cgtgaaaata acgaagtgac actggaagtt cgaaccaagt gtaagaatct
     2161 tgatgtttga aaatacgaac cggctaaaaa tgtaattttt gctttcagtt tatctccgca
     2221 aaaaatcatt tcggaatttg aacataaaac aagtaatctt gatgctcggc ttgaaaacat
     2281 taaagccgcg caagttcgag gcttcgtcac gcggctttgc tttgacccga ttatttattg
     2341 tcaaaattgg cgtgaggaat attcgtcgct tattgaaaaa acgcttgaag caattgacat
     2401 ctctgcagtt caggacatta gtattgggag tttccgaata agtcaaagct atttgaaaaa
     2461 tatgcgtgcg cgtgaccgga aaagcaaaat tgtcaacttc ccttttaaaa atgttggcgg
     2521 ttattatcaa tatgacgaag ctttgcaaac caagatggaa aatttcatgc tcgaaaattt
     2581 atcgaagcat tttgataagg agaaaatttt cttatggaaa taatttgcta cgcgctcgac
     2641 atagaagcaa tgcctttgaa aaaacatttc ggtgagaatg ttcgatttga gaagtgtgat
     2701 gtgttcgcaa aaaatttgga tttcctaaaa accgtttccg ccaacgatca cattacaaac
     2761 atcggaattt gcgcgggagc taatgtgggc gaaatttatt tgtgcaataa gattcacggc
     2821 gaaaaaactt tctatccaga tttgctaatc ccgaataatt tagaacaacg cgaaattaca
     2881 acagttgatc atgtagtcaa cgaagacgaa gtcaaggctc acccaaatat gctatatgac
     2941 caggaagctg caataatttt cgaacatgca caaaagtgca ttgcgccaca tcaaatttca
     3001 tttctcaaaa ttgtttctga ttccggaata actgattttg aaaaaattaa aaaatcaatt
     3061 cctcaattaa ttgaaaataa aattcctgaa attgaggcct tcatttcttc gtcacaaaaa
     3121 ttcttttcaa aaattgacac gaaggaagaa attaaaaacc tcgataaata ttcagagttg
     3181 cttaagtgta gcgccacaat gaaagtgcaa ctcgcgcaac ttctccgcta cgctcaaatt
     3241 tgcaacattg acgttgaaaa ttttttcaaa gacatcaaaa tatcttcaaa aaaagattct
     3301 ctacaaattt tgaaatcgct gaaaaatttc cttttatcag aagataagta aagaaatatt
     3361 gatttaagtc aagaaaattt tagtaatttg tagcaaaaag tatcctaaaa tggctcaaac
     3421 gcaaactgtg tacgaatatt atcaaattat tctgcatatt tttgattctt atattttttt
     3481 tctggtgttt tgtaaaattt aatcctaaaa acagggaaaa ctaagtaaaa atatgtacac
     3541 agtttgagtt tgagccattt ttcagggttt tcagctacaa atttaatgaa aaactatatt
     3601 ttctttgagc aaatctcaac tagaagaata gacttagtac aaatataaac gcgattgaga
     3661 ccacacccat gataagggtt gccacagttt gcgttttata accttgctct ggcgtcatgt
     3721 ctccgaattt tgttacaacc cagaagtagg aatcgttcgc gtgtgaaacc gtcattgcac
     3781 ctgcaccaat agccataacg caaagcacgg acattacttc ggagcccatg ccgagcgcag
     3841 ccatgaccgg cgccatcaaa ccggcagtgg tcgtaattgc aacggtcgaa gaaccttggc
     3901 tggatttcaa aatcgccgca ataatgaacg ggaagaaaat tccgacaggc tcaagcacag
     3961 cagcgttgtc agtaataaac tcaattaaac ttgtcgaagc aattaccttg cccagaactc
     4021 caccagccgc ggtcacgaac aaaattgggc cgacagtctt gagcgtttct tcggtgagtt
     4081 tgtaaatttt atcgcccatc ttcgatgtca ggagcaaaat aattccaagc acaacgccaa
     4141 ccgccaacgc gacaattgga gtgccaagaa actgccaaca agtgtttagc attccctccc
     4201 aaccgaggat ggtcgagatg tttgacattg ccattaaaag aattgggacc aaaattggag
     4261 caaccgacat ccatccgttt ggaagtttgc cgtaggattt tttcaactct tcgtaagttt
     4321 taaccacttc tttgtccgac gaagtttcgt ccgcgctttt gacttttttg ccgataaact
     4381 tcgcgtagaa ataggcggcg atcaaagcgg ggattgaaac tagcgctcca aatcccataa
     4441 caagaagaat gctgtttcca actccaagcg tgttagctgc tgcaataggt cctggggtcg
     4501 ggggtatgaa aacgtgggcc gcataaaggc cggccgccaa acacaaagtc atagttactg
     4561 acgaagccgc cgtgcgcttc accaaagctt ttcgaattgg gttgagcaca acaaaaccgc
     4621 tgtcgcaaaa aactggaatt gaaacgatcc atcccataag ctcaatcgca agcgcaggac
     4681 gcttttcgcc gacaagcttt acaacaagat cggcaagcgt gagcgcggca ccagttgctt
     4741 cgaggaagct cccgatcatc gcaccaaaaa taattacaat accgatgcta gtaaaagtgt
     4801 tcgcaaaacc agcaccaata acattcgcaa taccttgaac cttttctcca tcagcgccaa
     4861 cggtatcaac aagcgggatg ccgccgataa ttccaagcgc aagcgacaca agcaaaatcg
     4921 cgaggaacgg atgaacctta aacttggaaa tcatcacaat cattaaaata atcgcaatta
     4981 caaaaacaat taaaaatgga attccagtca tgttagcccc tactttcttt tacgacgacc
     5041 gtatttagaa cctttacgag tattttgttt caagcgctcg aggatgatgt cttcggaggt
     5101 gccttcgatg cttgcgcgaa gtttaaccac ttggtcgcga aggcgagctg cagcttcgta
     5161 atccatatct tttgaagcct gagccatgtc gtcttccagg gaagagatga tgcgaagcac
     5221 ttctgacttg gacaaatcgg agagttcagc gttcacctca tctgcgctac gtgcgacttc
     5281 gtcgctggaa acaaaactca tgatgctgtt gatggccttg cgaattgtct tcggctcgat
     5341 gccatgctct tcgttaaatt taacttgaat ggcacgacgc ctgttagtct catcaattgc
     5401 ggttttcatg ctgtcggtaa ttttttcgca atacataata acttcgccag aaacattacg
     5461 cgcagcacga ccaatcgtct gaataagaga gcgatgattt cgcaaaaagc cttccttatc
     5521 agcatcaaga atcgcaacaa gcgtgacttc cggcaggtca agaccctcgc gaagaaggtt
     5581 aatccccaca agaacatcaa attcgccagc gcgcaaatca ttaagaatct caacacgctc
     5641 aagcgttcca atatcgctgt gcatataacg cgcacgaatt tcgttatcaa gcaaatggtc
     5701 ggtcaa
//
LOCUS       DBNCDC010000075         5423 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_9020_length_5423_cov_7.290425, whole genome shotgun sequence.
ACCESSION   DBNCDC010000075 DBNCDC010000000
VERSION     DBNCDC010000075.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5423)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9020_length_5423_cov_7.290425"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(64..204)
                     /locus_tag="AC04VQ_05810"
     CDS             complement(64..204)
                     /locus_tag="AC04VQ_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553643.1"
                     /translation="MFFIGMVLYYPFIKIIDNKYIEEEKAIAAQNADPVDLDNIDYSE
                     IL"
     gene            complement(310..903)
                     /locus_tag="AC04VQ_05815"
     CDS             complement(310..903)
                     /locus_tag="AC04VQ_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIO9553644.1"
                     /translation="MPKSVPLTVVKSFEALIPAFLIIAVAFVIRIGMSFTPFGDLQQF
                     IFEVLQTPLTGLGNTLGAMVVAYLFLHFFWFFGINGSSVVGAVFNPILKTLAFENLEA
                     FKAGDPIPNIINQQFQDMFATLGGAGSTLSLIVLMIIICKSERIKKLSRLSIVPGCFG
                     INEPIIFGLPIVLNPLILIPFMLVPTVNIIISYYAMD"
     gene            complement(997..1209)
                     /locus_tag="AC04VQ_05820"
     CDS             complement(997..1209)
                     /locus_tag="AC04VQ_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553645.1"
                     /translation="MAIPASASFDVMALLAVGGIAYSLAKQFEEDAMQAALVAIVVFF
                     IVTPFVTQYLPDGATEPVDVTSIPIA"
     gene            complement(1219..1380)
                     /locus_tag="AC04VQ_05825"
     CDS             complement(1219..1380)
                     /locus_tag="AC04VQ_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553646.1"
                     /translation="MASAIAGNKYLIAIRDGFLLSVPLLIAGSFFLLIANFPIPAYLE
                     WMNSMFGET"
     gene            complement(1415..1753)
                     /locus_tag="AC04VQ_05830"
     CDS             complement(1415..1753)
                     /locus_tag="AC04VQ_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014368.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553647.1"
                     /translation="MDKRSILLFCDAGMSTSIMVKKMQEVCDNHNMPFEVSAHPIAKA
                     VDLVEEKNPAIVLLGPQVKFLEDKTRKQLEKFDVPVDVIDAAIYGAMNGEEVMKNVIR
                     KLREKKGNNE"
     gene            complement(1928..2695)
                     /locus_tag="AC04VQ_05835"
                     /pseudo
     CDS             complement(1928..2695)
                     /locus_tag="AC04VQ_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013292.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylasparaginase"
     gene            complement(2734..3048)
                     /locus_tag="AC04VQ_05840"
     CDS             complement(2734..3048)
                     /locus_tag="AC04VQ_05840"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014326.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HIO9553648.1"
                     /translation="MNYEEEVMGLIGAAGESKDMAFQALDALKDGDIAKAKELIAKSH
                     ESDVAAHNVQTKLISAEMDPEQEDVPMTLLMAHAQDHFMTTQLARELIEHFIDVYEAQ
                     KK"
     gene            complement(3058..4116)
                     /gene="ypdE"
                     /locus_tag="AC04VQ_05845"
     CDS             complement(3058..4116)
                     /gene="ypdE"
                     /locus_tag="AC04VQ_05845"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF007421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIO9553649.1"
                     /translation="MDVSLLEKLSNADALAGCENEVRDIMNKNLKGLCDEVSCDKLGS
                     IIFKKKGTNDGPKIIIAAHMDEVGFKVRTITEQGLIIVKALGAVKQLAKFHQNVRITT
                     NAGKKISGVLHSTYTEEEDIKNIPGKTYVDVGADNAQEVEKMGIEIGNIVCFDSQFEK
                     LEKKNLIKGKAFDDRIGCYVLARVLENLNGKKHPNTIYFCATSSEEVGTRGAQTCASM
                     INPDVAIVIDACCYKDEFDRSAVNVRQIGKGMILTHSDRGLEPNQKLLNLVKKCAKKL
                     NKNLQLDMFDRGSTDGLKIHLNGKGVPTQVCCVPLRYGHCAQSIANVQDIEDAIEIFT
                     ELICSIDEKVAKDFTTFL"
     gene            complement(4125..>5423)
                     /locus_tag="AC04VQ_05850"
     CDS             complement(4125..>5423)
                     /locus_tag="AC04VQ_05850"
                     /EC_number="6.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF037084.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III family protein"
                     /protein_id="HIO9553650.1"
                     /translation="ELAPIYTNANRAIDENLITMEKMQLLASKYNLACLLHERPFEGI
                     NGSGKHCNWSVCAGGFNLLDPGKTDSEIVRFIAFLSCVIAGVDEYQELIRASVASAGN
                     DCRLGGNEAPPAIISIYLGDALSNIINAVMSGHSYEAMTDSKMDLGVSVLPQIMRDSS
                     DRNRTSPFAFTANKFEFRMPGSQMNLSDCVMVLNTAIAKSIYDFNKLLEKGKSKIGNS
                     KKFQNFVLSCCAKTLKDHKRIIFNGDGYSSAWEKEAKKRGLSNNVTTADALPCMINKN
                     SVELFEAFGVLTEEELKSRYVISLEKYNKLINIESRLMHRMVKRTFLPAINEYASELS
                     SQINEISMAQNTFKLQNQKASLKKLLSGLEDIYDSLDKLDNLRDSAKAIKDEQKKANH
                     NAKKLVPAMKDLRHCVDSMEHIVPRDWWPVPSYNSMLFYV"
BASE COUNT         1757 a         1182 c          772 g         1712 t
ORIGIN      
        1 gacgcggacg tcatattata accaaaaata tgctttttag cacataaaat agtaaaccat
       61 aagctacaaa atttccgaat aatcaatgtt atctaaatca acaggatcag cattctgcgc
      121 agcaatggct ttttcttctt cgatgtactt attatcaata attttgataa acggataata
      181 caaaaccata ccaatgaaaa acaaaattat ctgcaaaact gcaccctgtc aaccactcac
      241 aagaaaaccg gatattatag gtggtgttgt tcacggaagc tgaattccat tacaaagcgg
      301 aaccaatcct caatccatcg cataataaga aataataata tttacagttg gtaccagcat
      361 aaatggaatc aagataagag gattaagtac aattggtaaa ccgaaaatta ttggttcatt
      421 gattccaaaa cagccaggaa caattgaaag acgagataac tttttaattc tctcactctt
      481 gcaaataata atcattaaaa caattagcga aagtgttgat ccggcaccac ccaaagttgc
      541 aaacatatct tgaaactgtt gattaataat atttggaatt ggatcccctg ccttaaaagc
      601 ctccaaattt tcaaaagcca aagttttcaa aattggatta aaaactgcac caacaacact
      661 gcttccatta attccgaaga accagaaaaa gtgcaagaat aaatatgcaa caaccattgc
      721 acctaaagtg tttccaagac cagtaagtgg agtttgcaaa acttcgaaga taaactgctg
      781 caaatctcca aaaggagtga agctcattcc aattcgaatt acaaaagcaa cagcaataat
      841 tagaaaagca ggaattagtg cttcgaaaga tttaacaaca gttagcggaa cactctttgg
      901 catatgtatt actcaacctc gttttacaaa ataaacgaag gctcgtgtag aaataagagc
      961 tgtaatcatt cctaaaaaca ttccttttga tcccattcat gcaattggaa tgcttgtaac
     1021 atcaactggc tcagttgctc catctggtaa gtattgagta acaaacggcg taacaatgaa
     1081 gaaaacaaca atagctacaa gagccgcttg cattgcatct tcttcgaatt gttttgccaa
     1141 actatatgca attcctccaa cagcaagcaa tgccataaca tcaaaactcg cacttgccgg
     1201 gattgccata tagtctgctc atgtttcacc aaacattgaa ttcatccact ctaaatatgc
     1261 aggaattggg aagtttgcaa ttagcaagaa aaaagaacct gcaattaaaa gtggaacaga
     1321 aagcaagaat ccatcacgaa tagcaatcaa atatttattt cccgcaatag ccgaagccat
     1381 cgggttaaag actttttcaa gtcagttcat aaatttattc attattgcct ttcttctctc
     1441 ttaatttgcg aattacattt ttcattactt cttctccatt catagcacca taaatagcag
     1501 catcaataac atcaactggc acatcaaact tttccaattg ctttcttgtt ttatcctcta
     1561 aaaatttcac ctgaggacca agcaaaacaa tagcgggatt cttttcttcg actaaatcaa
     1621 cggcctttgc aattggatgc gcagaaactt caaatggcat attatggttg tcacaaacct
     1681 cttgcatttt tttaaccata atgctcgttg acattcctgc atcacaaaat aacaaaatac
     1741 ttctcttgtc cataataatt cctttctata catccgcatg aatacgttta tcatatgcat
     1801 caagtcattt ctgactgatt ttttcaatct tagtagcgtt atttttttct ggccaagaaa
     1861 taaaaatttg tggatcctga ttttcgttag caacacaaaa tgtaaactca acagtagttg
     1921 aaacgcctca attgccaacc atatccatag ctatcaaact aatttgaaca tcgtcgatat
     1981 ttttgtttct gctctctaaa tcattaacaa attcataaag agttttatcg caagcatctt
     2041 gaggactcat tccatcttta attttacgaa caacttcaaa actcaagcaa cctttcataa
     2101 tgtcttcgcc aagacctgtt gcacttgccg ctccaatatt actatcagca taaaatcctg
     2161 agccagataa tggcgaatca ccaacgcgcc ctgggatttt cataaaaagc cccgaagtcg
     2221 aagttcctac cgcaattttt gaactcgaat caagcgcaat agctcccacg gtatcatgcc
     2281 cgtcataagc ctttaagtct ttttgcgctt ctagttccct tttcttattc tctcacatct
     2341 ttttagcacg ctctgttagc atatttgtta actcaaaatt attttcttct gcaaatttat
     2401 cagcatcatt tccaaccaaa aaattgttta ccttaaattc cgacagcttc ttcgcaacac
     2461 ttattggatt tttaattctc gaagccgcgc caaccgctcc aatttgaagc gtgtttccat
     2521 ccataaaagc tgcatcaagt tgaacttcgc aatttttgtt tggaagccca ccaaaaccta
     2581 cagatttata aaatgggtaa tcttcaacat ttttaattag agtttcaaca gcgtctgccg
     2641 cgtcttcgtt agctttcaac attttaaagg cttcaactaa accttcaaat gacattcttc
     2701 atgttgaaat taatccaata ctcataaaac ctcttatttt ttctgagctt cgtaaacatc
     2761 aatgaaatgt tcaatcaatt cacgagcaag ctgagttgtc atgaagtgat cttgagcatg
     2821 agccattaaa agagtcatgg gtacatcttc ttgctctggg tccatctcag cagatattaa
     2881 tttggtttga acattgtgtg cagcaacatc tgactcgtgt gacttcgcaa ttaattcctt
     2941 agcctttgca atatcaccat cttttagcgc atcaagagcc tggaaagcca tatctttgct
     3001 ttctccagca gcaccaatta aacccattac ctcttcttcg taattcatat taacctccta
     3061 taaaaacgtc gtgaaatctt tcgcgacctt ctcatcgatc gaacaaataa gttcagtaaa
     3121 aatctcaatc gcatcttcta tgtcttgaac gttagcaatt gattgagcac aatgaccgta
     3181 acgaagcggc acacaacaaa cttgagttgg aacccccttg ccatttaaat gaatttttaa
     3241 gccatcagta cttcccctat caaacatatc caattgaaga tttttattta actttttagc
     3301 acacttctta acaagattca aaagcttttg atttggttca agtcctctat cagaatgggt
     3361 taagatcatt cccttgccta tctgtcttac attaactgca gaacggtcga attcatcctt
     3421 atagcaacac gcatcaataa ctatagctac atctggatta atcatcgatg cacaagtttg
     3481 agcacctcga gtaccaactt cttctgaact tgtcgcacaa aaataaattg tattcggatg
     3541 ctttttgcca tttaaattct caagaactct tgctaaaaca taacacccaa ttctgtcgtc
     3601 aaatgcttta cctttaataa gattcttttt ctcaagtttt tcaaattgtg aatcaaagca
     3661 aacaatgttg cctatttcaa ttcccatctt ttcaacttct tgagcattgt cagctccaac
     3721 atcaacatat gttttacctg gaatattttt aatatcttct tcttcagtat aagttgaatg
     3781 taatacgcca gaaatctttt ttccagcatt tgtagtaata cgaacatttt gatgaaactt
     3841 tgcaagttgt ttaacggcgc ctaatgcttt aacaataatt aaaccttgtt ctgtaattgt
     3901 acgaacttta aatccaactt catccatatg cgcggcaata ataattttag gaccatcatt
     3961 tgtgcccttc ttcttaaaaa taatgctacc aagtttgtcg caacttactt catcacataa
     4021 gccttttaag tttttattca taatgtcacg aacttcattt tcgcaaccag caagagcatc
     4081 agcattactt aatttttcta ataaacttac atccataata tctcctatac ataaaacaac
     4141 atactgttat acgaaggcac cggccaccaa tcacgaggca caatatgttc cattgaatcc
     4201 acacaatgac gaagatcctt catcgccggt actaattttt tcgcattgtg attagccttc
     4261 ttttgttcat ccttaattgc tttcgcgcta tctcgcaagt tatcaagttt atctagactg
     4321 tcataaatat cttcaagtcc agaaagcaat tttttcaagc ttgccttttg attttgaagt
     4381 ttaaaagtat tttgagccat tgaaatttca ttaatttgag atgaaagttc tgatgcgtat
     4441 tcattaatag caggcaagaa tgttcgtttc accattctat gcatcaacct actctcaata
     4501 ttaatcagct tgttgtattt ttcaagcgaa atcacataac gacttttcaa ctcttcctca
     4561 gtcaaaactc cgaaggcctc aaaaagctca acgctgtttt tgtttatcat gcaaggaaga
     4621 gcatcggcag tagttacatt gtttgaaagc ccacgcttct tcgcttcctt ttcccatgca
     4681 gacgaataac catcgccgtt aaaaataatt cttttatggt ctttcaaagt ttttgcacaa
     4741 catgacaaca caaaattttg aaacttctta gagttgccaa tcttgctttt gcctttttct
     4801 aaaagcttat taaaatcata aattgatttt gcaatcgccg tgtttaaaac cataacgcaa
     4861 tcagataaat tcatttgcga accaggcatt ctaaactcaa atttattcgc agtaaatgca
     4921 aacggacttg ttctgtttct gtcagaagaa tcacgcataa tttgtggaag aacagaaact
     4981 cccaaatcca tttttgagtc tgtcattgcc tcgtaagagt gaccactcat aactgcgtta
     5041 ataatgttag ataaagcatc tcccaaatat attgaaataa tagctggtgg agcttcgttt
     5101 cctcctagcc tacaatcatt accagcgctt gcaacacttg ctcgaattaa ttcttgatat
     5161 tcatcaacgc ctgcaattac acaagacaaa aatgcaatga atctaacaat ttcgctatca
     5221 gtttttccgg gatcaagtaa attaaagcct cccgcacaaa cagaccaatt gcaatgctta
     5281 cccgaaccat taattccttc aaaaggacgc tcatgcaaaa gacaagccaa attatatttc
     5341 gaagcaagca attgcatttt ttccatcgtg attaaatttt catcaatcgc gcggtttgca
     5401 tttgtataaa ttggagcaag ttc
//
LOCUS       DBNCDC010000076         5357 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_9230_length_5357_cov_9.367974, whole genome shotgun sequence.
ACCESSION   DBNCDC010000076 DBNCDC010000000
VERSION     DBNCDC010000076.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9230_length_5357_cov_9.367974"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="AC04VQ_05855"
     CDS             complement(<1..883)
                     /locus_tag="AC04VQ_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIO9553651.1"
                     /translation="MGGTNLLGWMEKNHFDEIGSHFIMEVAKNNCGLLLPGCQPVYNP
                     MFGQWLYKNKKMYRDLAKWMPEFHKTGAKLFIQLTAGFGRSFTISEMMEKLYTNPFLR
                     VVSKPFMNLDKITATASPTPNRWSDKVPSRALTVDEIHEYVDAFAKTAKLIQEAGVDG
                     VEIHAVHEGYLLDQFTLGYVNSRTDEYGGSFENRYRFAVEIVQAIKKACGKDFPVSLR
                     YSAISKTKDFRKGAIPGEDYKEVGRDLKESEKAVKYLADAGYDMFNCDNGTYDAWYWA
                     HPPIYMPENCNLKDIEHL"
     gene            complement(976..2007)
                     /locus_tag="AC04VQ_05860"
     CDS             complement(976..2007)
                     /locus_tag="AC04VQ_05860"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIO9553652.1"
                     /translation="MKKFASVILSIVMVFCCVSISSCNGQKTPTQDNYDNVNKPVIGI
                     AWVQDRNEAFYQNWEYIIQKAGGIAVNLKQAKCYDIEYDVHDFVANSELGEEGMLGPS
                     AADRLKKCDKSHTNIEEVMRGIDGVFVPGGEDISPTLIKDFKVEENTCTGFSATRDVS
                     DGILEQYCIEKDIPMFCVCRGMQMLSVVCGGRMIQDIPDYCAENSINFDGKHRDTNKD
                     GPKEFVRHDVIVQDKDSTFYQQIVKADVFKNIPSWHHQCVHDVEGTDLREVAIDSGGT
                     FKVIEAVEMKNKSFILGTQFHPEVVLNRVYKQGLEDTCDKDICLRFFTTLVNIAEQKS
                     MTNPINRSS"
     gene            complement(2115..2837)
                     /gene="rplQ"
                     /locus_tag="AC04VQ_05865"
     CDS             complement(2115..2837)
                     /gene="rplQ"
                     /locus_tag="AC04VQ_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006363199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIO9553653.1"
                     /translation="MRHYKKGRKLGTDKSHTKAMKRSLVSALFLNDRIKTIESRAKEI
                     RPDVDKIIGWAKKGDLHSRRLAIAALGGDKYLVAEIFEKVAQGMFKERNSGYTRIIKL
                     GVRKGDNARIVIIELINEGKKKVAAKPADAKASDAKKDEAKAAAKPAEKKDEKKDEKK
                     PESKKEEKPAEKKEEKKEEPKKEEPKKEEKPAEKKEETKKEEKKEEPKKEDKKEEPKK
                     EDKKEEPKKEDKKEEPKKEENK"
     gene            complement(2860..3882)
                     /locus_tag="AC04VQ_05870"
     CDS             complement(2860..3882)
                     /locus_tag="AC04VQ_05870"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012799087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIO9553654.1"
                     /translation="MTEFIPPTIEIEEIKDNLSRFIVEPLEHGYGETLGNSMRRVLLN
                     SLTGAKVTAIKIEGVDHEFTTAPGVVEDVTDIVLNIKGLVFKCLDSVFNQSEGKDEND
                     LARILNADDFMATATIEASAPASGTYTVTGNDVKVPPEFEIVNKDHVICNIADGGELN
                     MELRLGIGRGRVVADDNKREDDPIGVIAIDSNFSPVRRCTMKKDDTRVGQRTDFDRLT
                     LEVETDGSTTPTEAVNMAALIINQHMVKFLDLVEAKEEEKKATIFAPEAEKENIELEK
                     RVEDLDLSVRSYNCLKRADIHTVRKLVEYSEKDLMNLRNFGTKSIDEVKAKLISMGLN
                     LRQEIG"
     gene            complement(3912..4544)
                     /gene="rpsD"
                     /locus_tag="AC04VQ_05875"
     CDS             complement(3912..4544)
                     /gene="rpsD"
                     /locus_tag="AC04VQ_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIO9553655.1"
                     /translation="MARYTGASCKLCRREGCKLFLKGPRCYSGKCAMERHPYPPGESG
                     KRRPRESQYRQQLREKQKTKRIYGVLEKQFRGYYETANRQSGVTGENLLRLLECRLDN
                     VVYRLGFSKSRAEARQQVRHGHINVNGKKVTIPSYRVRPGDLVSVVDKAKDLYNIQTS
                     IVENAKTSAIVPAWLEADIEHLQGSVISLPNRDQIDLDVKEQLIVELYSK"
     gene            complement(4562..4963)
                     /gene="rpsK"
                     /locus_tag="AC04VQ_05880"
     CDS             complement(4562..4963)
                     /gene="rpsK"
                     /locus_tag="AC04VQ_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006363202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIO9553656.1"
                     /translation="MAKGQKTTTTRVRRADKKNISVGAAHIKSTFNNTIVTISDPAGN
                     VISWQSAGTQGFKGSRKSTPFAAQMAAEQAAKTAMEHGLHRVSVYVKGPGSGRETAIR
                     SLQAAGLEVTSIQDCTPIPHNGCRVCKKRRP"
     gene            complement(4975..5337)
                     /gene="rpsM"
                     /locus_tag="AC04VQ_05885"
     CDS             complement(4975..5337)
                     /gene="rpsM"
                     /locus_tag="AC04VQ_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIO9553657.1"
                     /translation="MARLVGVDLPRDKRLEIGLTYIYGIGLSTSQKILKETGVSPDLK
                     CGDLTEEDLTKLRDYINDHVRSEGDLHRETSQNVKRLMEIGCYRGLRHRKGLPVRGQR
                     THTNARTRKGKKRIAGLK"
BASE COUNT         1502 a         1210 c          875 g         1770 t
ORIGIN      
        1 ttaagtgctc aatatccttt aagttgcaat tttcaggcat ataaattggt ggatgtgccc
       61 aataccaagc atcataagta ccgttgtcac agttaaacat atcatatcca gcatctgcca
      121 aatacttaac agccttttct gactctttca aatcacggcc aacttctttg taatcttcgc
      181 caggaatagc gcccttacga aagtccttcg tttttgaaat tgcagaataa cgaagcgaaa
      241 cagggaaatc ctttccgcaa gcttttttaa ttgcttgaac aatttccaca gcgaagcgat
      301 aacggttttc aaaacttcca ccatattcat cagttctgct attaacataa ccaagtgtaa
      361 attgatcgag caaataacct tcgtgaacag cgtgaatctc aacaccgtca acaccagctt
      421 cttgaatgag ctttgctgtt tttgcaaaag cgtcaacgta ttcatgaatt tcatcaacgg
      481 ttaaagcgcg cgaaggaact ttatcagacc atctgtttgg agttggactt gcagttgcag
      541 taatcttatc caaattcata aatggcttag aaacaacacg caaaaatgga tttgtgtaga
      601 gcttttccat catttcgctg attgtaaatg aacgaccaaa accagcagtg agctgaataa
      661 acaacttcgc accagttttg tgaaactctg gcatccactt cgctaaatcg cgatacatct
      721 ttttgttttt gtacaaccat tgaccaaaca ttgggttgta aacaggttgg cagcctggaa
      781 gcaaaagacc gcaattgttc ttcgcaactt ccataataaa gtgagaacca atctcatcaa
      841 aatgattctt ctccatccaa ccaagcaagt ttgtgccacc catcgatgta agaacgaatc
      901 tgttcttaat ctctaaattg ccaatctttc atggcgtaaa taaaggttct aatttcttgt
      961 ccataaaatt cctttctaac ttgacctatt tataggattg gtcattgact tttgttctgc
     1021 aatattaact aaagttgtaa aaaaccttag gcatatatct ttatcgcaag tgtcttctaa
     1081 accttgctta taaactctat taagtacaac ttcaggatga aactgagttc ccaaaataaa
     1141 gcttttgttc ttcatttcca ctgcttcaat tactttaaat gttccaccac tatcaattgc
     1201 aacttcacga aggtctgtgc cttcgacatc atgaacacat tgatgatgcc atgaaggaat
     1261 atttttaaat acatctgcct ttacaatttg ctggtaaaag gtggaatcct tatcttgaac
     1321 aataacatcg tgccgaacaa attctttggg gccatcctta ttggtgtctc tgtgctttcc
     1381 gtcaaaatta atgctatttt cggcacaata atcaggaatg tcttgaatca ttcgaccgcc
     1441 gcaaacaaca gagagcattt gcatgcctct gcaaacacaa aacattggaa tatctttttc
     1501 aatacaatac tgttctaaaa ttccatcaga tacatctcta gtggccgaaa atccagtgca
     1561 ggtattttct tcgaccttaa aatctttaat caatgttggg ctaatgtctt cgccaccagg
     1621 aacaaaaaca ccatcaatac ctcgcattac ttcttcaata ttagtatgtg atttatcgca
     1681 ctttttaagt ctgtctgctg cagacggacc caacatcccc tcttctccta gctcagaatt
     1741 agctacaaaa tcatggacat catattctat atcataacat tttgcctgtt ttaaattaac
     1801 agcaatgcca ccagcttttt gaataatata ttcccaattt tgataaaaag cctcgtttct
     1861 gtcttgaacc caagcaatac caattacagg tttattaaca ttatcatagt tatcttgagt
     1921 gggagttttt tgaccattac aagaagaaat agagacacaa caaaagacca ttacaatgga
     1981 taaaattacc gaagcaaact tcttcaattt tgtctccttt ttaagtttga atacaactta
     2041 acttgacacg cccattatag cataaaaaaa ggctgcgata cctcgcagcc tttgagttta
     2101 atatgaaatt taaattattt attctcttct tttttaggtt cttccttttt atcttccttc
     2161 ttaggttctt cctttttatc ttccttcttt ggttcttcct ttttatcttc cttctttggt
     2221 tcttctttct tttcttcttt tttagtttct tctttctttt cagctggctt ttcttccttc
     2281 tttggttctt ccttctttgg ttcttccttc ttttcttctt tcttctctgc tggtttttct
     2341 tccttctttg actcaggctt cttctcgtct tttttttcgt ctttcttttc agctggttta
     2401 gctgcggctt tagcttcgtc cttcttagca tcagaagcct tagcatctgc aggtttagca
     2461 gcaaccttct tcttgccttc gttgataagc tcaataatta cgatgcgagc attatcgccc
     2521 ttgcgaacac caagcttaat aatgcgagtg tatccgctat tgcgttcctt aaacatacct
     2581 tgagcaactt tttcgaaaat ctcagcaaca agatatttgt ctccgcctaa agcagcaatt
     2641 gcaagacgtc tgctgtgaag gtcgcccttc ttcgcccatc caataatctt atcaacatca
     2701 ggacgaatct ccttcgcacg agattcgata gttttaattc tatcatttag gaaaagcgca
     2761 ctaaccagac tgcgcttcat tgcctttgta tggcttttat cagttcctaa ttttctgcct
     2821 tttttgtagt gtctcataat gcaacttctc cttaattaat tagccaattt cttgacgcaa
     2881 atttaatccc atagaaataa gcttggcttt aacttcgtca atagattttg taccgaagtt
     2941 acgaagattc atcaaatcct tttctgaata ttcaacaagc ttgcgaactg tatgaatgtc
     3001 agcacgcttc aagcagttgt atgaacgaac agaaaggtcg aggtcttcga cgcgtttttc
     3061 aagctcaata ttttcctttt ctgcttctgg agcaaaaatg gttgctttct tttcttcttc
     3121 cttagcctca acaaggtcta agaatttaac catatgctgg ttaattatca aagctgccat
     3181 gttaacagct tcggtaggtg tagttgatcc atcagtttca acttccaaag tcaaacggtc
     3241 aaagtcagta cgttggccaa cacgagtatc gtcctttttc atagtgcatc gacgaactgg
     3301 gctgaagttt gaatcaattg caataacacc aattgggtca tcttcgcgtt tgttatcatc
     3361 agcaacaaca cgaccacgac caattccaag acgaagttcc atgttgagtt cgccaccatc
     3421 tgcaatatta caaataacgt gatctttgtt aacaatttca aactcaggtg gaaccttaac
     3481 atcgttacct gtaactgtat atgtaccact tgcaggagcc gaagcttcta ttgttgcagt
     3541 agccataaaa tcatcagcat ttaaaatgcg agccaagtca ttttcgtcct tgccttcgct
     3601 ttgattaaat acagaatcga ggcacttaaa aacaaggcct ttaatattta agacaatgtc
     3661 agttacatct tctacaacac caggtgcagt tgtgaattcg tgatcaacac cttcgatttt
     3721 aatagctgta accttagcgc cagttagcga atttaacaaa acgcgacgca tagaattacc
     3781 aagagtttca ccatagccat gttctagtgg ctcaacaata aaccttgata aattgtcttt
     3841 aatttcttca atctcaattg ttggtggaat aaattccgtc atgttgcctc cttaacttcc
     3901 ttaaaacttt attacttcga gtacaactcg acaatcaatt gctcttttac atcaagatca
     3961 atttgatctc tgtttggtag cgaaattacg ctgccttgca aatgttcaat gtcagcctca
     4021 agccaagcag gaacaattgc agaagtctta gcgttttcga caattgaagt ttgaatgttg
     4081 tataagtctt ttgccttatc aactacgctt accaaatccc ctggacgaac acgataagaa
     4141 ggaattgtaa cctttttgcc gtttacatta atatgtccat ggcgaacttg ctgacgagct
     4201 tcggcacgtg acttcgaaaa tccaagacga taaactacat tgtctaaacg gcactcaaga
     4261 aggcgaagca aattttcacc agtaacgccg ctttgacggt ttgcagtttc atagtatcct
     4321 ctaaactgtt tttccaaaac gccataaata cgctttgtct tttgcttttc acgaagctgt
     4381 tgtctatatt gactttcgcg aggacgacgc tttccagatt ctcctggtgg atatggatga
     4441 cgctccatag cgcactttcc agaataacaa cgaggtcctt ttaagaaaag cttgcagcct
     4501 tcccttcggc ataatttaca acttgcacct gtatatcttg ccatctacat tctccttcta
     4561 attatggacg tcttttcttg caaactctgc atccgttgtg tggaattgga gtgcaatctt
     4621 gaatacttgt aacttccaag ccagcagctt gaagcgaacg aatagcagtc tcacgaccac
     4681 ttcctggacc ctttacgtaa acacttacgc gatgaagtcc gtgctccata gctgtttttg
     4741 cagcttgttc agcagccatc tgagcagcaa aaggagtgga tttacgagaa cccttaaaac
     4801 cttgagtgcc agcgctttgc caagaaataa cattacctgc aggatcggaa atagtaacga
     4861 tagtgttatt aaaagtagat ttaatgtgag ccgcaccaac agaaatattt ttcttgtctg
     4921 ctctgcgaac tcttgtagtt gttgtttttt gtcctttagc catctacgta cctcctattt
     4981 caaacccgca atacgctttt tacctttacg agtgcgagcg tttgtatgag tacgctggcc
     5041 tcgaactgga agaccttttc tgtgacgaag acctctgtaa catccaatct ccatcaatct
     5101 tttaacattt tgtgaagttt cacggtgaag gtcaccttcg cttcgtacat gatcattaat
     5161 ataatcacga agctttgtca aatcttcttc ggttaaatct ccacatttaa ggtctgggct
     5221 aacacctgtc tcttttaaga tcttttgaga agttgaaagg ccaataccat aaatataggt
     5281 caatccaatt tcaagtcgct tgtcgcgagg aagatctaca ccaactaaac gtgccatcta
     5341 atctactcct ttatcct
//
LOCUS       DBNCDC010000077         5123 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_9955_length_5123_cov_6.837017, whole genome shotgun sequence.
ACCESSION   DBNCDC010000077 DBNCDC010000000
VERSION     DBNCDC010000077.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9955_length_5123_cov_6.837017"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /gene="dnaE"
                     /locus_tag="AC04VQ_05890"
     CDS             <1..1194
                     /gene="dnaE"
                     /locus_tag="AC04VQ_05890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIO9553658.1"
                     /translation="YFLIVQEYIAWARENGIGVGPGRGSAAGSIVSYVLGITDLDPLE
                     NGLMFERFLSPERVEMPDIDVDFDDDRREEVVEHVREVYGDDHVAKVITFGTLKAKNA
                     VRDAARVLDYPYGLGDDICKLIGDDLGVTIDSALANNIDLKKKYDTEPDAKKIIDTAR
                     ALEDRIRNEGVHACATIICRDPMSDHVPLKRAAGKNDDVITQYDGHFTPELGLLKMDF
                     LGLRTLGVLTRACRNVEARYGKHIVPEDIPIDDEKAFELIRSGNTDGLFQVESALYLS
                     LFSKIPPHKFSDIVVAIALNRTGPLESGMVDDYVRAVRGEKEVTYYDDRLKPILEETY
                     GSIVYQEQIMQISMEMCGFSAGKSDVLRKAIGKKKIDIMRDLKEDWCDGAVKNDYPLD
                     LANRI"
     gene            1264..2463
                     /locus_tag="AC04VQ_05895"
     CDS             1264..2463
                     /locus_tag="AC04VQ_05895"
                     /inference="COORDINATES: protein motif:HMM:NF025930.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIO9553659.1"
                     /translation="MRTAYMKAHYPNEFMAAVLTSYMTKNDRLIKYIASCNYNGTPVL
                     PPDVNVSNADFTPVDEGIRFGLVGLRGVGSDVAQAIVEEREKNGPYKHIHDFVNRLSS
                     KYCNHRVMEALVKAGAFDSTGYTRKQMWDFVENTNMLENARKHERERMHGQTTLFDMF
                     DEGDEAVAGFSDSVPPPDGIEWDKMELLKLEKEIMNMYVTDNPVRPYMPQITDETNYN
                     ISQLGELEHGIPKGTFGGMISDISTMRTKRGTLMSKFKLEDTTGSIEAICFDHEKNGI
                     NIAEDAIVFVKGKFEKADRGDQIIVYEVIPIVLDPNYKPKKQNVVVEKLSPLEIFITE
                     QDISEEKINRMNQTLQNNFGTQEVFLNISKADGKNIRAQMPFKVNAENGNLKPQISTI
                     FESVRFN"
     gene            2463..3122
                     /gene="truA"
                     /locus_tag="AC04VQ_05900"
     CDS             2463..3122
                     /gene="truA"
                     /locus_tag="AC04VQ_05900"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIO9553660.1"
                     /translation="MVICLDIAYFGEPFSGFARQPGQLTVQGSIEDALKTIFSEEVDT
                     TCAGRTDAGVHARHQFVSFESPKDDIDLDKLKRSLAQLTHDDIHINDVEFKDDDFSAR
                     FSAKAREYSYFICNQPNPSLYMRNTSWHIAKTLDVQSMQKAAQALLGEHDFKSFCVAK
                     SAEDKTTMRNISKIEFVEHEIFEEKFIEIIISGNAFLHSMVRTIVGTLIKVGLGQRDV
                     E"
     gene            3249..3500
                     /locus_tag="AC04VQ_05905"
     CDS             3249..3500
                     /locus_tag="AC04VQ_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553661.1"
                     /translation="MLQNKETIIFNELLNQLGVSADTLQDIVSSIARQPKSTFVLTKV
                     SGDMRIFYFEDENNHIFPISLDKLVEVKNLLMDMTGGVR"
     gene            3501..4352
                     /gene="ychF"
                     /locus_tag="AC04VQ_05910"
     CDS             3501..4352
                     /gene="ychF"
                     /locus_tag="AC04VQ_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIO9553662.1"
                     /translation="MALSIGIVGLPNVGKSTLFTAMTAKGGLAANYPFATIEPNVGVV
                     PVTDKRLDKLAEIDNPAQIVPATVEFLDIAGLVAGASKGEGLGNQFLANIRECNAIAE
                     VVRYFKDPDVVHVDGKVDPLSDVETIKTELILADIATIEKALPRMEKDAKRNKDEAFK
                     LESAKKALTGLNDGHRVRSLDLSDEEMDALYDLHLLTIKPILYIANCDEDQLTQEFDE
                     IDGCKPVPICAQVEADLAEMSEAEKQEYLEAIGLSESGLVRLVRNSYDLLGLQSFFTS
                     GEKETKA"
     gene            4365..4565
                     /locus_tag="AC04VQ_05915"
                     /pseudo
     CDS             4365..4565
                     /locus_tag="AC04VQ_05915"
                     /inference="COORDINATES: protein motif:HMM:NF017847.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
     gene            4573..5123
                     /locus_tag="AC04VQ_05920"
                     /pseudo
     CDS             4573..5123
                     /locus_tag="AC04VQ_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802594.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III domain-containing
                     protein"
BASE COUNT         1549 a          800 c         1195 g         1579 t
ORIGIN      
        1 tactttttaa ttgttcaaga atatattgca tgggctcgtg aaaatggtat tggtgttggg
       61 cctggtcgag gttctgctgc aggttcaatt gtttcatatg ttttgggaat tacagatctt
      121 gatccgcttg aaaatggatt gatgtttgag cgtttcttga gtcctgaacg tgttgagatg
      181 cctgatattg atgttgactt tgatgatgat agacgcgaag aagtagttga gcatgtccgt
      241 gaagtttatg gagatgatca tgttgccaag gtaattacat ttggaactct gaaagccaaa
      301 aatgccgttc gtgatgcggc gcgtgttttg gattacccat acggtcttgg agatgacatt
      361 tgtaaattga ttggtgatga tcttggtgtg accattgatt cggcgcttgc aaacaatatt
      421 gatttaaaga aaaaatatga tactgaacca gatgctaaaa agataattga tacggcacgt
      481 gctttggaag ataggatcag aaacgaaggc gtgcatgcat gtgcgacaat tatttgtcgt
      541 gatccgatga gtgatcatgt tccactaaag cgtgctgcgg ggaaaaatga tgatgtaatt
      601 actcaatacg atggccactt tacgcctgag cttggcctgt taaagatgga ttttttgggt
      661 cttcgtactt tgggagtgct cactcgtgca tgtaggaatg ttgaagcacg ctatggtaaa
      721 catatagttc ctgaagatat tccaattgat gatgaaaaag cttttgaatt aattcgaagc
      781 ggtaatactg acggtctatt tcaggttgaa agtgctcttt atttaagttt gtttagtaaa
      841 attcctcctc ataaatttag tgacattgtt gttgcaattg ctttgaacag aactggtcct
      901 cttgaatctg gaatggttga tgattatgtt cgtgctgttc gcggtgagaa ggaagtaact
      961 tattacgatg atagacttaa gccaattttg gaagagactt atggatctat agtttatcag
     1021 gagcagatca tgcagatttc tatggaaatg tgtggtttta gcgctggtaa atctgatgtt
     1081 ttgcgtaagg ctattggtaa gaaaaagatt gatattatgc gtgatttgaa agaggattgg
     1141 tgtgatggtg ctgttaaaaa tgattatcct cttgatcttg cgaatagaat ctgagacgat
     1201 gttgaaaaat tcgctaaata cgcttttaac aaatctcact ctgctgctta tgcaattttg
     1261 gttatgcgca cggcttatat gaaggctcat tatccaaacg aatttatggc tgctgttctt
     1321 acaagttata tgactaaaaa tgacagactg attaaataca tcgcaagttg taattacaat
     1381 ggcacgcctg ttcttccgcc ggatgtaaat gtttcaaatg ccgattttac tcctgtagat
     1441 gaaggtattc gcttcgggct tgttgggctt cgaggtgtag gaagcgatgt cgcgcaagct
     1501 atagtggaag agcgcgagaa gaatggtcct tacaaacaca ttcatgattt tgtcaaccgt
     1561 ttaagttcga agtattgcaa tcacagggtt atggaggcgc ttgtaaaagc gggtgccttt
     1621 gatagtacag gttatactcg caagcagatg tgggacttcg ttgagaatac gaacatgcta
     1681 gaaaatgctc gcaaacatga gcgtgaacgt atgcatgggc agactacttt gtttgatatg
     1741 tttgacgaag gtgacgaagc cgttgccggc tttagcgatt ctgttccgcc gcctgatggt
     1801 atcgagtggg ataagatgga gcttttaaag cttgaaaaag aaatcatgaa tatgtatgta
     1861 actgataacc ctgttcgtcc atatatgcct caaattacag atgaaacaaa ttacaatatt
     1921 tcacaattag gcgagcttga gcatggaatt ccaaaaggta cttttggtgg aatgataagt
     1981 gatatttcaa ccatgagaac taagcgagga actttaatga gcaagtttaa gcttgaagat
     2041 actactggct caattgaagc tatttgtttt gatcatgaaa agaatggtat taatattgct
     2101 gaagatgcga ttgtttttgt gaaaggcaaa tttgaaaaag ctgacagagg ggatcaaatt
     2161 atagtttacg aagtaatccc aattgttctt gatccaaatt acaaaccaaa gaaacaaaat
     2221 gttgttgtag aaaagctttc gccccttgag atttttatta ctgagcaaga catctccgaa
     2281 gaaaagatta atcgtatgaa tcaaactctt caaaataatt ttggaacaca agaagtattt
     2341 cttaatattt ctaaagctga tggaaaaaat attcgtgcac aaatgccttt taaggtaaat
     2401 gctgaaaacg gaaacttaaa accgcaaatt tcaactatct ttgaaagtgt tcgctttaac
     2461 taatggtaat ttgtttagat attgcgtatt ttggagagcc tttttctgga tttgcgaggc
     2521 agcctggcca attgacagta cagggatcga ttgaagatgc gttaaaaaca atctttagcg
     2581 aggaagttga tacgacctgt gcaggacgaa ctgatgctgg tgttcacgcg agacatcagt
     2641 ttgtaagttt tgaatctcct aaagatgata ttgatcttga caagttgaag aggtcgcttg
     2701 cgcaattaac tcatgatgat atccatataa atgatgttga atttaaagac gatgatttct
     2761 ctgcacgttt tagtgcgaag gctcgcgaat attcttactt tatttgcaat caaccaaatc
     2821 cgtcactata tatgagaaat actagttggc acattgctaa gacattagat gttcaatcta
     2881 tgcaaaaagc agcgcaagct cttcttggag agcacgattt taaaagcttt tgtgttgcaa
     2941 agagtgctga agataaaact actatgcgta atatttctaa gattgagttt gttgaacacg
     3001 aaattttcga agagaaattc attgaaatta taatttcggg aaatgctttt ttgcatagta
     3061 tggtaagaac gattgtcgga actttaatca aagtaggttt aggccaacgt gatgttgaat
     3121 gagtcgaaga ggttttgcta gcgcaaaatc gacagtctgc aggggaatgc gcacctgcaa
     3181 aagggcttgt tttaatgggt gtttattact aaattgtgat aaaatctcaa ttggagattt
     3241 tattaaccat gcttcaaaac aaagagacca taatttttaa tgagctgctt aatcagcttg
     3301 gcgtaagtgc tgatacattg caagatattg tcagttcaat tgcaagacag ccaaaatcta
     3361 cttttgtctt gacaaaagtg agtggagata tgcgaatctt ttattttgaa gacgaaaata
     3421 atcacatttt ccctatttca ctcgacaagc ttgttgaggt taagaatttg ttaatggata
     3481 tgacaggcgg tgttagataa atggcattat cgattggaat tgttggactt ccaaatgttg
     3541 gcaaatcgac tctttttaca gcaatgactg cgaagggagg tttggctgca aattatcctt
     3601 ttgcgacaat tgagccaaat gttggtgttg ttcctgtaac tgacaagcgt ttagataagc
     3661 ttgcagaaat tgacaaccct gcacaaattg tcccagctac tgttgaattt ttagacattg
     3721 caggacttgt tgcaggagcg agcaaaggcg aaggcttggg caaccagttc ctcgcaaata
     3781 ttcgtgagtg caatgcaata gctgaagttg ttcgttattt taaggacccg gatgttgttc
     3841 atgttgatgg caaggttgat ccacttagtg atgtggagac aatcaagacc gaacttattt
     3901 tggctgacat tgcaacaatc gagaaggctt tgcctcgcat ggaaaaagat gctaaaagaa
     3961 ataaggatga agcatttaaa ctcgaatcag cgaaaaaggc acttacgggt ttgaatgatg
     4021 gtcatcgtgt ccgttctctt gacttgtctg acgaagaaat ggatgcgctt tacgatttgc
     4081 atttacttac gattaaacca attttataca ttgccaattg tgatgaagat caacttacac
     4141 aagagtttga tgaaattgat ggatgtaaac ctgtgccaat ttgtgctcag gtcgaggcag
     4201 atctagccga gatgagcgaa gctgaaaagc aagaatactt agaagcaatt ggtttaagtg
     4261 aaagcggatt agttcgcctc gttagaaatt cttatgattt gttggggctt caatcatttt
     4321 ttacgagtgg tgaaaaggaa acgaaggcat gaacggtccg caaaggggcg aaggctccag
     4381 aggctgctgg agttattcat acagattttg agcgaggctt tattaaagcc gaaactgcaa
     4441 gctatgctga ctatattgaa tgcggtggcg aagcaggatg taggaacgct ggtaaacttc
     4501 gtcaagaagg caaggattat gttgttcaag atggcgatgt aatgcatttt aaatttaacg
     4561 tttaggggca aaatgacaaa cagtaaaaac acaaaatttt attccacgct tgtttttgac
     4621 gatagcacaa tgaaaaaata tttggacgag aagtcttata aagattttcg tgatattgtt
     4681 gaaaatggaa agcctttaag tataaatttg gctgataaaa ttgcagtggc catgaaagat
     4741 tgggctgtta gcaagggagc aactcatttt actcactggt ttcaacctct taatggaaca
     4801 acttcagaaa agcacgacag ctttttagtt cctgaaggtg atagtggtaa ggcaatattt
     4861 agttttacgg ggaaggctct aattagcggc gaaggtgatg cttcgtcttt cccatctggt
     4921 ggacttcgtg caacttttga agcgcgaggc tacacagctt gagacccaag ctctaatgca
     4981 tttattaaag acgaagtttt atgcattcca actgcatttt gtagttataa cgggcaggcg
     5041 cttgacaaga agactccgct tcttcgttct atggatgcaa ttaaagctca gctgacagtt
     5101 ttcatgaatt attttggagt gaa
//
LOCUS       DBNCDC010000078         4855 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_10963_length_4855_cov_9.850625, whole genome shotgun sequence.
ACCESSION   DBNCDC010000078 DBNCDC010000000
VERSION     DBNCDC010000078.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10963_length_4855_cov_9.850625"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="AC04VQ_05925"
     CDS             <1..1158
                     /locus_tag="AC04VQ_05925"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIO9553663.1"
                     /translation="HPPIYMPENCNLKDIEHLKKFTDKPLVCAGRMDPEVAAESIKAG
                     KLDGAGFARNFLSDQEWITKLMNDQKEDIRPCILCHNGCFNMCHYKGVPNDQSLEDSL
                     HLARCAINAETMAWDKHYMTPTNEPKTVHVIGGGIGGMEAALRLKQRGHTPVIYEKTD
                     KLGGTFIPASAESYKGKLRDLLAWYIREVKKAGIEVKFNTEIKAEEVAKFGKDPVVVA
                     TGSTPRQLKMVKGYEKMIEACEYLRGEKKVGDNVVVVGGGLTGSEIAYELALQGKKVT
                     IVEMLDDLIKQKGVCLANSSYLREWFELNKVPVYLETTLAEVNDGSIKCKKKDGSMVD
                     IKCDSVISSAGYIPTPISDGKKKNVYLVGDCDHIGNLRHAIWRAYEVAMDI"
     gene            1168..2442
                     /locus_tag="AC04VQ_05930"
     CDS             1168..2442
                     /locus_tag="AC04VQ_05930"
                     /inference="COORDINATES: protein motif:HMM:NF016310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HIO9553664.1"
                     /translation="MELTKEQQDILNGSKGETMAKVLETMVRYGELFGATKMVPVSSK
                     YNHLVTSFGMKTLNPVFELLDKILDAGEKSSQEFSVDPRPVDPKVPASLIEKFVFKKF
                     MYNMQDEYEEQLRKFGLMNDDAFTCTCYLPEVGNTPKKGEVLSWAESSAVVFANSVLG
                     ARCNRNSGIIDLMGSVVGFVPYFGLLTDEGRKATWKIEIKTSKMPEAQLLGSAVGMKV
                     MEDVPYIVGLDKWIGDKLDDKTKTYLKDFGAATASNGAVGLYHIENLTPEAKDQGEKL
                     LAKGYKTFVIDDKVLEDTYKNYPIIWKDINAAPKLCFMGCPHFSLDQLKEWTEKLEKG
                     LEKYGNKKVQIPTVFTAAPGVVKAFQKTDYYERLTGMGVIISSICPLMYMNNPLAKKK
                     PVITSSNKLRTYTTARFYMDDEILEQLVKGGK"
     gene            2442..2894
                     /locus_tag="AC04VQ_05935"
     CDS             2442..2894
                     /locus_tag="AC04VQ_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014091.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X swivel domain-containing protein"
                     /protein_id="HIO9553665.1"
                     /translation="MASKTFKGRVVAKGTIEAPALVSHGGLNTLASFQNALQWGDKTA
                     KCGDQGNKDLYNKAMAGKALCLPQTIGSTTGGLVLYCACSMKRQPACMLFSEPIDSLA
                     AAGAILADVWVDDVTMPVVDSLGEDFLNYVKDDMKISVKENGEVVVES"
     gene            2932..3663
                     /locus_tag="AC04VQ_05940"
     CDS             2932..3663
                     /locus_tag="AC04VQ_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009140015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIO9553666.1"
                     /translation="MCPLCFLGGFVDSIAGGGGLITLPAFLAVGLPSHIAIGTNKIQS
                     TLGTLVSTIRYFRQGYVDIKIAIACAIMAIIGSAIGSNLVLYVDDWTLKIILIILLPI
                     TAFFVLWNKGMSKKRKEYGFTKTIIIAAVVAFVIGTYDGFYGPGTGTFLILLLTIIGH
                     MTLENANGISKAANLASNIAAAVVFLINGVPLIALGLTAGLFNAAGNFFGSKLFIDKG
                     IKVVKPIMIVVLILLFAKVLFDMFV"
     gene            3673..3819
                     /locus_tag="AC04VQ_05945"
     CDS             3673..3819
                     /locus_tag="AC04VQ_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553667.1"
                     /translation="MKKLIGIFTVVCVLTLCIFASSCSFQTQAKTYSVPNNNEIQVVN
                     YNPV"
     gene            3856..4026
                     /locus_tag="AC04VQ_05950"
     CDS             3856..4026
                     /locus_tag="AC04VQ_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553668.1"
                     /translation="MSVNAKQNGAKMVIFPAMSLSGFMTENDEATAQSCAIELNDGKI
                     SPIAKTADEKDL"
     gene            4213..4371
                     /locus_tag="AC04VQ_05955"
     CDS             4213..4371
                     /locus_tag="AC04VQ_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553669.1"
                     /translation="MLNFPELTQYYSALVDNTYNHLCNVRTCNLVVGCLALNEGCSIA
                     SPNYKPAE"
     gene            4435..4632
                     /locus_tag="AC04VQ_05960"
     CDS             4435..4632
                     /locus_tag="AC04VQ_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553670.1"
                     /translation="MCGTQNNELFPGGSFIYQTYVDENDSFKRALFAGGKQGKFDINL
                     FMGSEFADRIFPFEISSDCES"
     gene            4657..>4855
                     /locus_tag="AC04VQ_05965"
     CDS             4657..>4855
                     /locus_tag="AC04VQ_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553671.1"
                     /translation="MIDGSIAPKFYSNTKLYANLYKELADKYDSGELKDEKYPTHTDI
                     GVATVNTCCPVWGNKKSGLENM"
BASE COUNT         1455 a          757 c         1068 g         1575 t
ORIGIN      
        1 catccaccaa tttatatgcc tgaaaattgc aacttaaagg atattgagca cttaaagaag
       61 tttactgata agcctcttgt ttgtgctggt cgtatggatc cagaagttgc agccgaaagc
      121 attaaggctg gtaaacttga tggtgcgggc ttcgctcgta atttcttgtc cgatcaagag
      181 tggattacaa aacttatgaa tgaccaaaaa gaggatattc gtccttgcat tttgtgtcat
      241 aacggttgct ttaatatgtg tcattataaa ggtgttccaa atgatcaaag tttggaagac
      301 agcttgcact tggcacgttg tgccatcaat gcagaaacaa tggcttggga taagcactat
      361 atgactccaa ctaatgaacc taagaccgtt catgttattg gtggaggtat tggtggcatg
      421 gaggctgctc ttcgattgaa gcagcgtggt catacacctg taatttatga aaagactgac
      481 aagcttggtg gaactttcat tcctgcttcg gcagaaagtt ataagggtaa gcttcgtgat
      541 ttgttagctt ggtatattcg cgaggtcaaa aaggccggaa ttgaagttaa gtttaacacc
      601 gaaataaagg ccgaagaagt tgccaagttt ggtaaagatc cagttgttgt tgctactggt
      661 tcaacacctc gtcaattaaa gatggtaaaa ggctatgaga aaatgattga ggcttgcgaa
      721 tatcttcgtg gcgagaaaaa agtaggagac aatgttgtag tagttggtgg cggtcttact
      781 ggttctgaaa ttgcatatga acttgcactt caaggtaaga aggtcaccat tgttgagatg
      841 cttgatgacc ttattaaaca aaagggtgtt tgcttggcta attcatctta tttgcgtgag
      901 tggtttgaac ttaataaagt tccagtttat ttggaaacaa ctcttgctga agtaaatgac
      961 ggttcaatca agtgcaagaa aaaagatgga agcatggttg acattaaatg cgatagcgtt
     1021 atttcttctg ctggatatat tccaacacca atttcagatg gcaagaagaa aaatgtttat
     1081 cttgtaggtg actgtgatca tattggcaat ttgcgccatg ctatttggcg tgcgtatgaa
     1141 gtcgccatgg acatctaagg aggggaaatg gaattaacta aagagcaaca agatatatta
     1201 aacggctcaa aaggcgaaac tatggccaaa gttttagaaa ccatggttag atacggtgag
     1261 ctttttggtg ccacaaaaat ggttcctgtt tcaagcaagt acaatcatct tgtaacttcg
     1321 tttggaatga aaactttaaa tcctgtgttt gaacttttag ataaaatttt ggatgcaggc
     1381 gaaaaatctt ctcaagagtt cagtgtagat ccgcgtcctg tcgaccctaa agttcctgca
     1441 agtcttattg agaagtttgt tttcaaaaag tttatgtata acatgcaaga cgaatacgaa
     1501 gagcagcttc gtaaatttgg tcttatgaac gatgatgcat ttacttgtac ttgttatttg
     1561 cctgaagttg gaaatactcc taagaaaggt gaagtgttat cttgggctga atcaagtgct
     1621 gtagtttttg caaattcagt tttaggagca agatgtaaca gaaacagcgg catcattgat
     1681 ttgatgggca gcgttgttgg cttcgttcct tacttcggtc ttttgactga cgaaggacgt
     1741 aaagctactt ggaaaataga aattaagact tctaagatgc ctgaggctca gctattaggt
     1801 tctgctgttg gaatgaaggt tatggaagat gttccatata tcgttggtct tgataaatgg
     1861 attggcgata agcttgatga caaaaccaag acttatctta aagattttgg cgcggccact
     1921 gcttcgaacg gagctgttgg tttatatcat attgaaaacc ttactcctga agcaaaagat
     1981 caaggcgaaa aacttcttgc taagggttat aagacttttg taattgatga caaagtttta
     2041 gaagatactt ataagaacta tccaatcatt tggaaggaca ttaacgctgc tcctaaactt
     2101 tgctttatgg gttgtcctca tttcagctta gatcagctta aggaatggac agaaaaactt
     2161 gaaaaaggcc ttgagaaata cggcaacaag aaagtccaaa ttcctactgt atttactgct
     2221 gcacctggtg ttgttaaggc cttccaaaag acagattatt atgagcgcct aacaggaatg
     2281 ggtgtaatta tctcgtctat ttgtccgctt atgtatatga acaacccact ggcgaagaaa
     2341 aagccagtta tcacttcgag caataaactt cgcacttata ctactgctcg tttttatatg
     2401 gatgacgaaa tccttgagca attggttaaa ggaggtaaat aatggctagc aaaactttta
     2461 agggcagagt agttgccaaa ggcacaatcg aagctccagc tttagtctcg cacggtggcc
     2521 ttaatacatt agcttccttt caaaacgcgt tgcagtgggg agataaaact gcaaagtgtg
     2581 gcgaccaagg caataaagat ttatacaaca aagccatggc aggtaaggca ttatgcttgc
     2641 ctcaaactat tggttctact actggtggtt tggttttgta ttgcgcatgc tcaatgaaac
     2701 gtcaaccagc ttgtatgctt tttagcgaac ctattgattc gcttgcagct gcaggtgcaa
     2761 ttttagctga cgtttgggtt gacgatgtta ctatgccagt tgttgattct ttgggagaag
     2821 attttctcaa ttatgttaaa gacgatatga aaatttctgt taaagaaaat ggcgaagtag
     2881 ttgtagagtc ttaagatctt ggttgaagtt agtttttgaa cttttttagt aatttgcccc
     2941 ttatgctttt taggtggctt tgtagattct attgcaggag gcggtggatt aatcacgctt
     3001 cctgcttttt tggcagttgg cttgccttcg catattgcaa ttggaacaaa taaaattcaa
     3061 tcaactttag gcactcttgt ttcaacaatt cgttatttta gacaaggtta tgttgatata
     3121 aagattgcaa ttgcttgcgc gataatggca ataattggtt ctgctattgg ttcgaattta
     3181 gttttatatg ttgatgattg gaccttaaaa attatcctga taattctttt gcctattaca
     3241 gcattttttg tgctgtggaa taagggtatg tcaaaaaagc gaaaagaata tggctttaca
     3301 aaaacaataa ttattgctgc agtagttgct tttgttattg gaacttatga tggcttttat
     3361 gggcccggga caggcacttt cttaattttg ctgttaacta ttattggtca catgacttta
     3421 gaaaatgcca atgggatttc taaagcagca aatttggcaa gtaatattgc tgccgctgtt
     3481 gtcttcttga ttaatggagt tccgcttata gccttaggtt taactgcagg tttgtttaat
     3541 gctgcgggca atttcttcgg atcaaagctg tttattgata agggcattaa agttgtaaag
     3601 ccaataatga ttgttgtttt aatattgctt tttgctaaag tattgtttga tatgtttgtt
     3661 tagaggtttt atttgaaaaa gttaattgga atatttactg ttgtttgcgt tttgacatta
     3721 tgcatttttg cgtcttcgtg ttcttttcaa acgcaagcaa aaacttattc agtccctaat
     3781 aacaacgaaa ttcaagttgt aaattacaat cctgtttgag gaaacgttga tgctaatgct
     3841 gaaaaaatta tccagatgtc tgtgaatgca aagcaaaatg gtgcgaagat ggttatcttt
     3901 ccagcaatgt ctttgtctgg ttttatgaca gaaaatgacg aagccacagc tcagtcttgt
     3961 gcaattgagt taaacgatgg caaaatttca ccaattgcaa aaactgccga cgaaaaagac
     4021 ttgtgaatta tcttcggagc tattgagaaa aagaataatt cattgtttaa ttcgacaatt
     4081 gcaatttctc ctgaaggttt tgcaagtgtt tacgaagaaa tggtaccaac ttcgaattta
     4141 tttgaagcag gtagctatcc tgtttgcata gatactgagt gaggttatgt tggtttgaca
     4201 tgcggtaatg atatgttaaa tttccccgag ctaactcaat actattcagc tttagttgat
     4261 aatacctata atcatttatg caatgtccga acatgtaatt tggttgttgg atgtttagca
     4321 ttaaacgaag gatgtagcat tgcttcgcca aattataagc ccgctgaatg aacattacta
     4381 aatagagttt gtgccgtcag tgaaaataat tacatgttgt gtgctgttgc aaatatgtgc
     4441 ggcacacaaa ataacgaatt gttcccaggc ggatctttta tatatcaaac ttacgttgat
     4501 gaaaatgata gttttaaacg agctttattt gctgggggta aacaagggaa gtttgatatt
     4561 aatttattta tgggatctga atttgcggat aggatttttc cttttgaaat aagcagtgat
     4621 tgtgagagtt gaccgaaaga cttcattggt actaaattga ttgatggttc aattgcgcct
     4681 aagttttatt ccaatacaaa actttatgcg aatctttata aagaacttgc agataaatat
     4741 gacagtgggg aattaaaaga tgagaaatat ccaactcaca ctgatatcgg agtagctacc
     4801 gtaaatactt gttgtccagt ttggggcaat aaaaaatctg gtcttgagaa tatgg
//
LOCUS       DBNCDC010000079         4844 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_11002_length_4844_cov_5.064523, whole genome shotgun sequence.
ACCESSION   DBNCDC010000079 DBNCDC010000000
VERSION     DBNCDC010000079.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11002_length_4844_cov_5.064523"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            18..1127
                     /locus_tag="AC04VQ_05970"
                     /pseudo
     CDS             18..1127
                     /locus_tag="AC04VQ_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013406.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            complement(1360..2334)
                     /locus_tag="AC04VQ_05975"
     CDS             complement(1360..2334)
                     /locus_tag="AC04VQ_05975"
                     /inference="COORDINATES: protein motif:HMM:NF025042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase type-2 family protein"
                     /protein_id="HIO9553672.1"
                     /translation="MTNSACRALKDNYVGGICITKYDHSQGEIPRIKIYEAGHPTPDE
                     STVLATQAAINYARRLGEDDTVLFLVSGGGSALFECPNCSLEDFIKINDELLASGKNI
                     KEMNSIRKQYSNVKGGKFAEICKPAKVINLILSDVLGDDLKSIASGPTIAEGVDSYLV
                     GNLSKLIEAAAKACNDLGYRPIIFNDNMTGYAKEEAESFANEAIKYSKSKEKAALISG
                     GETVVKLQGSGKGGRNQEFALACAPLIEGKNITIFSVGSDGTDGPTDAAGGFASGESM
                     KLYHDAEIDVQAYLDNNDSYHALKATNQLIITGPTGTNVNDIAVALIN"
     gene            complement(2338..2472)
                     /locus_tag="AC04VQ_05980"
     CDS             complement(2338..2472)
                     /locus_tag="AC04VQ_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553673.1"
                     /translation="MNELIDKIISASIEAVLPDKSVEDALASIELNGNIHVIAIGKAA
                     "
     gene            complement(2485..2997)
                     /locus_tag="AC04VQ_05985"
     CDS             complement(2485..2997)
                     /locus_tag="AC04VQ_05985"
                     /EC_number="3.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF019559.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HIO9553674.1"
                     /translation="MYDKLIIAGLIFGLVGDVCLNLRHVLKKIGDKIFLCGVAAFLIG
                     HVIYLIAVIPSATRIITDLVAGVIIAAVLLIYIFKTMKVKIAFKIFGIFYLGAIIIMT
                     TIAIDAAFASPVLKNLLFAFGAAMFTASDIVLIFNTFGGNETFPRRITNLSLYYIAQI
                     CIALTTMFVA"
     gene            complement(3175..3723)
                     /locus_tag="AC04VQ_05990"
     CDS             complement(3175..3723)
                     /locus_tag="AC04VQ_05990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HIO9553675.1"
                     /translation="MKHVYLFLADGTEECEALITVDLLRRAGVDVNTVSVMDDICVKT
                     SSGIKMEADVMIGGVNLDEADMLILPGGMPGTKYLGECEKLCETVKEFNEQGKLLAAI
                     CAAPSVLGKLGVLNGKKAICYPGFEDALEGATISDVDPVTDGNVTTASGLGHAIQFAL
                     EIIRVLCGNEAGTKVAQEIGYI"
     gene            3829..4368
                     /gene="eda"
                     /locus_tag="AC04VQ_05995"
     CDS             3829..4368
                     /gene="eda"
                     /locus_tag="AC04VQ_05995"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - (R,S)-4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HIO9553676.1"
                     /translation="MGIFEKIKEIGVVPVIALDDPALAPGLANALCDGGLPIAEVTFR
                     MDNVAEVMNAMKQARPEMTLGAGTVLTKKQVDEAIEAGCEFIVSPGLNDEVTKHCIDK
                     GIPHIPGVATPSEVERAMSFGHKTLKFFPAELNGGLAYIKALCAPYRDVEFMPTGGVN
                     PSNLKEYLSFEKIAAAGGT"
     gene            4462..4821
                     /locus_tag="AC04VQ_06000"
     CDS             4462..4821
                     /locus_tag="AC04VQ_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIO9553677.1"
                     /translation="MRVVTFGEIMMRLAPQGYDRFIQSNKLDMTFGGGEANVCVSLAN
                     YGIDASFVTKVPEHDIGQCVVNELRRYGVDTSYIARGGDRLGIYFCEKGASQRASNVI
                     YDRAHSAIATAENKDFD"
BASE COUNT         1411 a          949 c         1014 g         1470 t
ORIGIN      
        1 ttcgatgaat ccctgacatg tttaaatatg tcaaagacaa aaaggcgact ccaggcaaaa
       61 agacgatgtc ttcgtttcaa gctttaatgg tctccactgg ctctcgtgta ggcactggaa
      121 atattgctgg tattgctaca gctatcataa tgggaggtcc aggtgctgta ttttggatgt
      181 gaattctagc gattgtaggt gcgtcttcgg cgtttgcaga atcaaccttg gctcagctct
      241 tcaaacgaaa gaacggcact ggaggatttc gcggaggtcc agcatactat atcgaacaag
      301 gattaaagaa gcgatggctc ggtgtcacct ttgctgtggc tctaatgttt gcgttcgcac
      361 ttgggtttaa tgcacttcaa acttttaacg ctgtatcggc cttagactat tattttaaag
      421 acccagccta cgacattaat atgatccatc ttgttgctgg ttttgtcatt atgattttaa
      481 ctgccctgac aatttttgga ggaatgaaat ccattggtaa tgtttcttcg gtaattgtgc
      541 ctttcatggc attgctctat atcgctcttg cattgctaac catttttact aaccttggct
      601 caatgggcga agtttttgct gtaatgttta ataatgcttt tagcgttgag tctatagtag
      661 ggggagcagc aggcgcggca ttgctttatg gaaccaaacg tggtctttta tctaacgaag
      721 ccggcatggg ttctgctcca aatgcagctg caagcgcaag tgtaactcat cctgttaagc
      781 aaggattaat tcagtccctt tcagcctata ttgatacttt ctttatttgc acaagttcgg
      841 cattcatggt tatgatttat gtagttcagc aacctgaatc tgcctacgcc ctaaacggaa
      901 tgcctcttgt tcaacaggcg ctaacaagta cggttggaaa ttttggaata cactttatga
      961 cagttgcaat tttcttcttt gcgttttcaa gcatcatcgg aaactatagt tatgccgaga
     1021 gtaacttcac ttttatatct aagaatccaa gatcgctgta tctatttaga ggcatttgtt
     1081 tgcttccaat tattctgggt tcgatgatga gtcttgatct tgcgtgaggc attgctgata
     1141 tctttatggg aatcatggca atcatcaaca ttatttcctt gttgttccta gggaagtgag
     1201 tctttaagct tctaaaggac ttcgacaagc agaagaagca ggggctcaat cctgtattct
     1261 atgttgatga gcataaggga tatccgaaga ttgagtgttg gcatgtgact cgcgaagaaa
     1321 tcatgaccgc ccgcgcaaag cggaacaaat aacaactaac tagtttatta atgcgactgc
     1381 gatgtcgttt acgttggttc ctgttggtcc tgttatgatt aattgattag ttgcttttag
     1441 agcgtgatag ctgtcgttgt tgtctaaata agcttgaaca tctatttctg cgtcgtggta
     1501 taacttcata gattcgccag aagcgaagcc tccagcggcg tctgttggac cgtcggtgcc
     1561 gtcgcttccg acactgaata ttgtaatgtt tttgccttcg ataaggggag cgcaggcaag
     1621 ggcgaattct tgatttcttc cgcctttacc agaaccttgc aacttaacga cagtttcgcc
     1681 gccagatatt aaagccgcct tttctttaga ctttgaatat ttaattgctt cgttagcgaa
     1741 gctctcagct tcttctttag catatccagt catattatcg ttgaaaataa ttggtctata
     1801 gccaaggtca ttacaggctt tagctgctgc ttcgattaat ttagaaagat taccaactaa
     1861 ataagaatcg acgccttcgg cgatcgtagg acccgaagca atagacttta aatcatctcc
     1921 aaggacatct gaaaggatta agttaataac ttttgcaggt ttacaaattt ctgcgaattt
     1981 gccgccttta acatttgaat attgctttcg gatggagttc atttctttta tgttttttcc
     2041 gctagcaagc aattcatcgt taattttaat gaaatcttct aacgagcagt tggggcattc
     2101 aaataaggca cttcctccgc cagaaacgag aaacaagacc gtatcatctt cgcctaaacg
     2161 gcgagcataa ttaatagcag cttgagttgc aagaacagtg ctctcgtcag gagtagggtg
     2221 tccggcttcg tagattttaa ttcgaggaat ttcaccttga gaatgatcgt actttgttat
     2281 acaaattcct ccaacataat tatcttttag agctcgacaa gcagagtttg tcatcgctca
     2341 ggcagctttg ccaattgcta ttacatgaat gtttccgtta agctcaatag aagctaaagc
     2401 gtcttcgaca cttttatcgg gcagtacggc ttctatcgaa gcagagatta ttttgtcgat
     2461 tagctcgttc ataaattcac tcgcttatgc tacaaacatt gttgttagag caatgcaaat
     2521 ttgagcaata tagtaaagcg acaagtttgt aattctgcgc gggaaagttt cgttgccgcc
     2581 aaaagtattg aaaatcaaaa caatgtctga tgcggtaaac attgcagcgc caaaagcaaa
     2641 caaaagattt ttcaggacag gagacgcaaa tgctgcatct atggcaatgg ttgtcataat
     2701 tattatcgcg ccaagataga aaattccaaa aattttgaac gcaattttaa ccttcattgt
     2761 cttaaatatg tagattaaaa gcacggcagc aataattact ccagctacaa ggtctgtaat
     2821 tattcttgta gcgctaggaa ttacagctat taaatatatt acgtgaccaa tcaaaaaagc
     2881 agctacacca catagaaata ttttgtcgcc aatttttttc agaacatgac gaaggtttaa
     2941 acacacgtct ccaactaaac caaaaattaa accagcaatt attagtttgt catacatcga
     3001 gaaagctgtc ttcgttgagc atcaaacacc taaggaaaca aagaagacag aagcaaggcc
     3061 tttaagaaca acagccaaag ttcaattttt cttgtactcg gcaaacataa atattccttg
     3121 cgaaatcatg ccaagcacta ttagaacaat cataatgata ttcatttaaa ccccttagat
     3181 atatccgatt tcttgagcta cttttgtacc tgcttcattt ccgcaaagga ccctaataat
     3241 ttctaaggca aattgaatgg catgaccaag gccgcttgcg gttgtaacgt ttccatcagt
     3301 tacaggatca acatcactga tagttgcacc ttctaacgcg tcttcgaaac cagggtagca
     3361 aatagctttt ttaccattaa gcacacctaa ttttcctaaa acactagggg cagcacaaat
     3421 tgcagcgagt agtttgcctt gctcattaaa ctctttaaca gtttcacaaa gtttttcgca
     3481 ctcgccaaga tattttgttc caggcattcc tccaggcaag atgagcatat cagcttcatc
     3541 aagatttaca ccgccaatca taacatcagc ttccatcttg attccactgg atgttttaac
     3601 gcaaatatca tccattacag aaacagtgtt tacgtcaacg ccggcgcggc gaagtaaatc
     3661 aacagtgatc aaagcttcac attcttctgt gccgtctgct aaaaataaat atacatgttt
     3721 catgattttc ctttcctcga cgtttatatt ttacaacaaa tattttaaat acgtaaaatt
     3781 aattgatggt ataatcagca ggtgaaataa taataaattg gagaagttat gggtattttt
     3841 gagaagatca aagagattgg tgttgttcca gttatcgctc ttgacgatcc tgctttggca
     3901 ccaggtcttg ctaatgcgct gtgcgatgga ggccttccaa ttgctgaagt aacttttaga
     3961 atggacaatg tggctgaagt gatgaatgcc atgaaacaag ctcgtccaga aatgacactt
     4021 ggggcgggta ctgttttgac caaaaaacaa gtcgatgaag caattgaggc aggctgtgag
     4081 tttattgtaa gtcctgggct taacgatgaa gttactaaac actgtataga caaaggtatt
     4141 ccgcacattc caggagttgc cacgccttcg gaagttgaac gagctatgtc ttttggacac
     4201 aaaacgttaa agttcttccc agcagagctg aatgggggct tggcttatat caaggctttg
     4261 tgcgctcctt acagagatgt tgaatttatg ccaactggcg gagttaaccc ttcgaaccta
     4321 aaagaatatt tgtcttttga gaaaattgcg gcagcaggcg gaacatgaat cgcaaaaaca
     4381 gccatgatta aagctgaaaa gtttgacgag atagaaaatc ttgcacgcgc ggcaaggggc
     4441 attgttgaag aggtgagata aatgagagta gttacatttg gtgaaataat gatgaggctt
     4501 gctcctcaag gttatgatag gtttattcaa agcaataaac ttgatatgac atttggaggc
     4561 ggcgaagcta atgtttgtgt ttcacttgca aattatggaa tagatgcaag ttttgtaacg
     4621 aaggttcctg aacacgacat aggacaatgt gttgttaacg aacttagaag atatggcgtt
     4681 gatacttcct atatcgcaag aggaggcgat cgccttggaa tttatttttg cgaaaaaggt
     4741 gcaagtcagc gagcaagcaa tgtgatctac gacagggcac attcggcaat cgccacagca
     4801 gagaataaag actttgattg agctcaagtt tttaaagatg ctga
//
LOCUS       DBNCDC010000080         4843 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_11004_length_4843_cov_6.572682, whole genome shotgun sequence.
ACCESSION   DBNCDC010000080 DBNCDC010000000
VERSION     DBNCDC010000080.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11004_length_4843_cov_6.572682"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(1..1152)
                     /locus_tag="AC04VQ_06005"
                     /pseudo
     CDS             complement(1..1152)
                     /locus_tag="AC04VQ_06005"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
     gene            complement(1267..2040)
                     /locus_tag="AC04VQ_06010"
     CDS             complement(1267..2040)
                     /locus_tag="AC04VQ_06010"
                     /inference="COORDINATES: protein motif:HMM:NF020132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIO9553678.1"
                     /translation="MTKRVAVLDDIAGFGKCSLGIAIPVLNAADIEVYPGITSLYSTH
                     TPIKGYTKLDTGTFLDDTIASWKKLNVNFDAILTAAITSKEQIYACMQLFADNPNAIK
                     VVDPVLGDFGDFYDDDFKALLPHLKKLCTHADIITPNQTEAKLLTGGNPEAITELGAK
                     NAIVTGLVRDDKIINTLINAEGSQEFSSPYIDEDIHGAGDLFDSALVAKIAKGTNIEE
                     ATDFATKITFDAVKLTVGQADFKAKGIAFEPVLKEIANI"
     gene            complement(2033..2257)
                     /locus_tag="AC04VQ_06015"
     CDS             complement(2033..2257)
                     /locus_tag="AC04VQ_06015"
                     /inference="COORDINATES: protein motif:HMM:NF042815.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6724 family protein"
                     /protein_id="HIO9553679.1"
                     /translation="MDISFLNTIGEKLSEVGKFLFETIPGVAILIGAFLLITLLIAVI
                     LEVRTKNKFKNHPIEEDEAEASQDEEDSND"
     gene            complement(2264..2347)
                     /locus_tag="AC04VQ_06020"
     tRNA            complement(2264..2347)
                     /locus_tag="AC04VQ_06020"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2312..2314),aa:Leu,seq:taa)
     gene            complement(2413..3786)
                     /locus_tag="AC04VQ_06025"
     CDS             complement(2413..3786)
                     /locus_tag="AC04VQ_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012544.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIO9553680.1"
                     /translation="MGFTCVWGFGNVANGFGYFGGTGVIVPWILVFVLYFAPYALMVG
                     ELGATFKDKAGGVSSWIQETIGPKLAFFCGWIYWVVHTPYMSQKPNNMIIAANWAFFQ
                     NGSVSDYEVWAVQLIGLGIFILCVFLATRGISVIKVISSVAGMASFAMGLLYILMVFT
                     APAINPGVQLNPIDFKLESFIPSDATMLLNLSVLIFAVGGIEKLSPYVNQMKNGKREF
                     PMGMIFVVVMVTVCAFLGTFAMGMMFGPNNVPDGFLTNGQYQAFQMLGNYYNVGNLFV
                     IIFAITNFITNFAVLIISLDAPLRIMLGNSDKKFIPAALLKTNKHGAYTNGILLEFVI
                     VSILILIPCLGISDIDALVKWLIKLNSVCMPLRYLFVFIAYIALKKAAGKFKNDGYTF
                     TKNKTLGLIAGGWCFLVTAISCLSGMYSEDMFQLAANILTPVVLLALGLILPKIANRN
                     KKAPAKK"
     gene            3988..4413
                     /locus_tag="AC04VQ_06030"
     CDS             3988..4413
                     /locus_tag="AC04VQ_06030"
                     /inference="COORDINATES: protein motif:HMM:NF016839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIO9553681.1"
                     /translation="MNLKNIKNINLKDNLKEAVVTSANGGHKRLLITALVLGCLVFSI
                     CFLYQPARDFYISVREQDRLQAQYDALKNTNTQLQNDIEYLQTEEGIKQRASETLGLI
                     QAGESIGYVAGSEIADGRDNSAASTSSKLAYKNIKTPET"
     gene            4448..4723
                     /gene="yihA"
                     /locus_tag="AC04VQ_06035"
     CDS             4448..4723
                     /gene="yihA"
                     /locus_tag="AC04VQ_06035"
                     /inference="COORDINATES: protein motif:HMM:NF014474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIO9553682.1"
                     /translation="MISVSSAKFIASYATAEQLPASTKPEVSFVGRSNVGKSTLINKL
                     CNNKKLAKVSSTPGKTTAINFFDAGDIYLVDLPGYGFAKRSKGERAR"
BASE COUNT         1431 a          980 c         1046 g         1386 t
ORIGIN      
        1 gtaatcttcg ttgacaaatt tgtctgcctt cgcttttagt gcaaagtaag cagcaaatac
       61 aaacaagtaa cggattggca tacatacact gtttagatcg attagccatt ttacaagtcc
      121 gttggcatcg ccgattccaa agcatggaat gattgtgatg attgaaacaa ttattgtaac
      181 aaccaggatg ccgtttgtgt agcttccgta tttattttgc tttaaccacc attttggaat
      241 gaagtttgta tctgcatttc caagaaggat acgtagtggc gcatcgatgc tgataatgat
      301 tgctgagaat tgggcgagtg cgttggctgc cgcgtagaag aacattagcc agtcgccaag
      361 accaaagaaa tatccaagtt tgcaaaatgc ttgatattgt ccgttggcca agaagtctgc
      421 gttgtcttta aggatttctt gtccatcgta gaaagaattc attgcaattg ttccaagtac
      481 tgcagttgca aaaaccataa tgacaagaat gatcattcct tttgggaaac ctttgcctgg
      541 ttccttcata gacttaacat aaggagcaat tttttcgcaa cctccaacag ccaagattag
      601 aattgacata ttaacaagaa ggctcatgtc atttggccaa agtccgtctc gtgctatatc
      661 aaatgtgcgg agttcgccag tgtgctctgg attaatgaga ggtgcagcaa aaatcataag
      721 aatgtataag aacatcatta caagcattgc aataccagca atcttggcaa tgtttttaac
      781 catgtctgcg cctcggaggg acagcacaac accaactaag aagactgcta agcatagaag
      841 ttgcaacact gtaacatcca tgccggctat tgtgccttga gggctgaaaa ttcagttaag
      901 ggagacgacc atcatgttgg ccttctgagt tagatatggc atatgaacaa cccaataaat
      961 ccatcccgcg aaaaatgcga cctttggacc tattgtttga aagcaccaaa cgctaacgcc
     1021 accgtcggtc tttttaaata ctgatccaag ttctccaacc attagtgagt aaggcaggaa
     1081 gtaaagaaca aaaacggcaa gccacggaag gataacttgt gtgcctccaa agtagccaaa
     1141 gccgttgcag atgttaccga agcttcaaac tccggcaaat gccatcagcg caaggccgaa
     1201 tcctgttagg ttgtttttag gtttaacctg ttttgtctta gatgctaatt tagccatgtc
     1261 atctccttaa atatttgcaa tttcttttag cacaggctca aaggcaatgc ctttagcttt
     1321 aaagtctgct tgaccaactg ttaattttac cgcatcgaag gtgattttgg tagcaaagtc
     1381 tgtggcttct tcaatatttg tgcctttggc aatttttgcc acaagggcgc tatcgaatag
     1441 gtctccggcg ccatgtatgt cttcgtcaat atatgggctt gaaaattcct gcgagccttc
     1501 ggcgtttatt agggtgttta tgattttatc atcgcgaaca agacctgtaa ctattgcgtt
     1561 tttggctcca agttctgtga tagcttcggg gttgccaccc gtgagcagct tcgcctcggt
     1621 ttggtttggc gtgataatat cggcgtgagt gcaaagcttt tttaggtggg gcagaagcgc
     1681 tttgaaatca tcgtcgtaga aatcgccaaa gtcgccaagg actgggtcta caactttaat
     1741 tgcgtttggg ttgtctgcga aaagttgcat gcacgcataa atttgctcct tgcttgtaat
     1801 ggccgctgtt aggattgcat cgaagttgac gtttagcttt ttccaagagg ctattgtgtc
     1861 atccaggaaa gtgccggtgt ctagcttcgt atagcccttg attggcgtgt gagtgctata
     1921 taggcttgtg attccagggt agacttcgat gtcagcggca tttaggacgg gaatagcaat
     1981 gccaagggag cacttgccaa agcctgcgat atcatcaagg actgcaacgc gcttagtcat
     2041 tggagtcttc ttcgtcttgg gaggcctcgg cttcgtcttc ttctattgga tgatttttga
     2101 atttgttttt agtgcgaact tcaaggataa cagctataag caaagttatg agcaggaacg
     2161 cgccaattag aattgccacg ccaggaatag tttcgaagag gaacttgcca acttcgctta
     2221 gcttttcgcc aattgtattt aaaaatgaaa tgtccattgt ttctggtaga ggcgggggga
     2281 tttgaacccc cacaggttgc cccggcggat tttaagtccg ctgcgtctgc cgttccgcca
     2341 cgcctccaaa aattagcgcc acatatttta tattaaatac gtggcgctga tgtgacaatt
     2401 tctaaaaatt aattattttt tagcaggtgc ctttttgttt ctgtttgcaa tctttggaag
     2461 gataagtcca agtgcaagca aaacaactgg agtaagaatg tttgcagcta attggaacat
     2521 atcttccgaa tacataccag ataagcaaga gatagcagtt acaaggaagc accaaccacc
     2581 agcaattaag ccgagtgtct tgttcttcgt aaatgtgtag ccatcattct tgaattttcc
     2641 agcagccttc ttcagcgcaa tgtatgcaat aaatacaaac aagtaacgaa gtggcatgca
     2701 aacagaattg agcttaatga gccatttaac cagagcatcg atatcgctaa ttccaagaca
     2761 tggaataagg ataaggattg aaacgattac aaactcaagc aaaataccgt ttgtgtaagc
     2821 gccatgcttg ttagtcttga gaagagcagc agggatgaac ttcttgtccg aattaccaag
     2881 cataatacga agtggagcat ccaagctgat aatcaaaaca gcgaagtttg taatgaagtt
     2941 ggtaattgcg aagatgataa cgaataggtt accaacgttg tagtaattgc ctaacatttg
     3001 gaatgcttga tattgaccat ttgtaaggaa gccatcaggt acattgtttg gtccaaacat
     3061 catgcccatt gcaaaagttc cgaggaacgc acaaaccgta accattacaa caacaaagat
     3121 catacccata gggaattcgc gtttgccgtt cttcatttgg ttaacataag gcgatagctt
     3181 ctcaattccg cctactgcaa aaattaggac ggataggtta aggagcatcg ttgcatcact
     3241 tggaataaag ctttcaagct tgaagtcaat aggattaagt tgaacaccag gattaattgc
     3301 aggagcggtg aaaaccatca aaatgtagag tagacccatt gcaaaacttg ccataccagc
     3361 aacgcttgaa ataaccttaa ttacgctaat tcctcgagtt gcaaggaata cacaaagaat
     3421 aaaaattcca agaccaatta actggacagc ccaaacttca taatcactaa cgctgccgtt
     3481 ctggaaaaat gcccagtttg cagcaataat catgttgttt ggtttttgtg acatgtaagg
     3541 tgtgtgaaca acccagtaaa tccagccgca gaaaaatgcg agctttggac cgattgtttc
     3601 ttgaatccac gaagaaactc caccagcctt gtctttaaat gtggcgccaa gttcgccaac
     3661 catgagggcg taaggggcaa agtaaagcac gaataccaaa atccatggca cgataactcc
     3721 agtacctccg aagtaaccga aaccgttagc tacgttacca aatccccaaa cgcaagtaaa
     3781 gcccatcaat gcaagacttc aaactgaaat cttcttgctt gcgggcgctt ttgagtttga
     3841 ctttttatta gccataattt tcctttctaa atgcgtgagg tggaattata ccaatttttc
     3901 taaattgttg actataaaat tataatgtgt gctaaaattt gctacaactg agaaaaattg
     3961 aataaaggca gtttaaacgt agaataaatg aatttaaaaa atatcaaaaa tattaatctc
     4021 aaagataatc ttaaagaggc tgttgttacc agcgcaaatg gtggtcataa acgtttatta
     4081 attactgctt tggttcttgg ttgtcttgtt ttttcaattt gctttttgta tcaacctgct
     4141 cgtgatttct atatcagcgt tcgcgagcaa gataggttgc aggctcaata cgacgcttta
     4201 aagaatacaa atactcaact tcaaaatgat attgaatacc ttcaaaccga agaaggcatc
     4261 aagcaacgtg cgagcgaaac tcttggtctt attcaagcgg gtgagtcgat tggttatgta
     4321 gcgggaagcg agattgccga tggtcgcgat aattccgcgg cgagtacgtc ttcgaagctt
     4381 gcttataaaa atatcaaaac tcccgaaacc tgatattcgc ctgttctaaa tttcattttt
     4441 aacgttcatg attagcgtaa gttccgcaaa gtttattgcc agctatgcga ccgcagagca
     4501 gctgccggca tccacaaagc ccgaagtttc ttttgttggg cgatcaaatg ttggtaaaag
     4561 cacgctaatt aataagcttt gtaataacaa aaagttggcg aaagtttcgt ccactcccgg
     4621 caagactact gcaattaatt tttttgatgc aggggacatt tatttggtag accttccagg
     4681 ctacggattt gcgaagcgct caaaaggcga gcgtgcgcgt tgatcttcgc ttattgatga
     4741 atattttaat caagaacgca actttgaaat ggtttatgtt ctcctcgaca ttcgccacga
     4801 tccaagtgat ttggatttgc aaatgattgc gtacttgaga aac
//
LOCUS       DBNCDC010000081         4810 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_11140_length_4810_cov_5.142376, whole genome shotgun sequence.
ACCESSION   DBNCDC010000081 DBNCDC010000000
VERSION     DBNCDC010000081.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11140_length_4810_cov_5.142376"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(215..346)
                     /locus_tag="AC04VQ_06040"
     CDS             complement(215..346)
                     /locus_tag="AC04VQ_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553683.1"
                     /translation="MFEDCTSLTKAPYLPAETLVERCYDEMFKNCESLQEVSVGFSI"
     gene            complement(872..1234)
                     /locus_tag="AC04VQ_06045"
     CDS             complement(872..1234)
                     /locus_tag="AC04VQ_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553684.1"
                     /translation="MFKVGKHQNVYSRFLNLFTNKKILLGLLAAAIVAILIPTISFAT
                     EVMDAEGETLPEKVENAINEIIGEPSATPAAANADMSNYLCFTPEGSSATFKFGPDYS
                     PPGKIINKVEYSVNEGSS"
     gene            complement(1302..1826)
                     /locus_tag="AC04VQ_06050"
     CDS             complement(1302..1826)
                     /locus_tag="AC04VQ_06050"
                     /inference="COORDINATES: protein motif:HMM:NF025019.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HIO9553685.1"
                     /translation="MPVYNCTEPVSPLLLNKKSNLFKLFLNDVGLLTTMFGKTVIKKI
                     LNGKTDINFGAVYENFVAQEFNSHIINCYYFNNKKFGEIDFIIEYDGVLPIEVKSGAS
                     YTKHRALDAVMSNKAYNIKKAYVFSNENLSHADISTVKKAGRNFEFKDKYRVVYLPIY
                     MTMFIDESAIDLTT"
     gene            complement(1848..2642)
                     /locus_tag="AC04VQ_06055"
     CDS             complement(1848..2642)
                     /locus_tag="AC04VQ_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024571.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIO9553686.1"
                     /translation="MIGNKASLLIDGARQVGKTTTIRKVLNDENIDYIELNLLENKDL
                     VEMLENIENISTAEFIAQLKLYTNHGLKKKETIIFIDEVQQCCELITKVKFLVEEGSF
                     RYIFSGSLLGLELANVKSVPVGYMFQLKMFPLDFEEFLLNTGVDNDTIKILETSFKNL
                     SPVSPFIHKQIMKRFDQYLVVGGMPKAVQTFVDTHDYNQVYDIHQFIIDAYKADFTKY
                     ENNSKKLLLKAAFDNIPAELNSQNKRYIVKNLNDARHYKRLEETFE"
     gene            complement(2816..3073)
                     /locus_tag="AC04VQ_06060"
     CDS             complement(2816..3073)
                     /locus_tag="AC04VQ_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553687.1"
                     /translation="MSFWQFEKDTRIFNKAQETTKLAQDKLGFQNLEAGDYFLGWKDR
                     DDVFRFCHNFTNDEVDAIASKFNVIDSFTADKLNKYIVLSK"
     gene            complement(3494..4810)
                     /gene="lysS"
                     /locus_tag="AC04VQ_06065"
                     /pseudo
     CDS             complement(3494..4810)
                     /gene="lysS"
                     /locus_tag="AC04VQ_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012379.7"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
BASE COUNT         1385 a          981 c          899 g         1545 t
ORIGIN      
        1 ctggatctat tggagtaatt gttattccgt gttcttgccc atcatataca ttattgtatc
       61 cttcaacttt agcagaaaca aaatattgca ctgttcaatt ttgagggacg tggctagcat
      121 ctcttgttgt cggaagagct ttcggacact taaaaacacc agtatttgaa acgttcgcta
      181 accaattgct cgttgagcct aaaccaacat catttcaaat agaaaatcct acggaaactt
      241 cttgcaaact ttcacaattt ttaaacattt catcataaca tcgctcaaca agagtttcag
      301 caggtagata cggcgcttta gttaatgaag tacaatcttc aaacatgcta gaatagcaag
      361 aatcaactaa attgagcgct ggtaattctg gggctgttct taatgctgaa catccactga
      421 acattcagag ataacatcag tcgcctaatt tggttgccgg taaagcaggg ggctcttcta
      481 aagatacaca gtcctcaaac attctataat aagaaccatc actaacttca atagctggta
      541 attctggagc gaccgataaa ttcgtacaac ccctaaacat acaacaataa caatctgagc
      601 ttaaagtggt cgcgggtaaa tctggaggtg taagcagtcc agtacatcaa gaaaataaat
      661 attgaaaaca gtaagggata ctctttatag acaaaagcgt tccctctccg ttatctatta
      721 aagacataac gttcccactt gctttaattt tattagtttc atcaatagta aaaacgtagc
      781 ttctatcatc tgtattaaag ccgtcaggat tataagacct aatgtatgtt gggcctgtaa
      841 ttgtaatatc ctcttgaaga tttaactcta ttcaggaaga tccttcatta acagaatatt
      901 caactttgtt aattattttg cctggtggac tataatccgg tccgaattta aatgttgcgc
      961 tagagccttc gggggtgaag cagaggtagt ttgacatgtc tgcatttgcg gctgctggag
     1021 tagcagatgg ttcgccgata atttcattga tggcgttttc aaccttttcg ggcagtgtct
     1081 cgccttcggc gtccatgact tcggttgcaa aagatatggt cggaataaga attgcaacaa
     1141 ttgcggcagc taatagtccg agtaaaatct ttttgtttgt gaataagttt aagaatctag
     1201 aataaacgtt ttggtgtttt ccaactttaa acataattac tcctttttct aaacgcatta
     1261 ttatactata aattaactaa ttattgagat agaacttgtg gctaggtggt caaatctata
     1321 gcagattcgt caataaacat cgtcatataa ataggcagat atacaactct gtatttatct
     1381 ttaaattcga aatttcgacc tgctttttta actgtggata tatctgcgtg ggataaattt
     1441 tcattggaaa acacatacgc cttttttatg ttgtatgcct tgttagacat aacagcgtct
     1501 agggccctat gttttgtata gcttgcacca gactttactt cgatgggcaa aacgccatca
     1561 tattctatta taaagtcaat ctcgccgaat tttttattgt tgaaataata gcaatttatg
     1621 atgtgcgagt taaattcttg agcaacgaag ttttcataaa cggctccgaa gttaatatcc
     1681 gtttttccat ttaatatttt tttaataaca gttttgccaa acattgtagt gagcaggcca
     1741 acatcattta ggaacagctt aaacaaattc gattttttat tgagtaacag cggagatacc
     1801 ggctctgtgc aattataaac aggaatagaa acacctgcag cattatgtca ttcaaaagtt
     1861 tcctcaagtc gcttatagtg acgagcgtcg ttcaggtttt taacaatgta tcttttattt
     1921 tgggaattta gttccgccgg aatattgtca aatgcggctt taagaagaag ctttttcgaa
     1981 ttgttttcat actttgtaaa atctgctttg tacgcatcaa taataaattg atggatgtca
     2041 taaacttggt tataatcgtg cgtgtcaacg aaggtttgca cagcttttgg cataccacca
     2101 acaactaaat attgatcaaa acgcttcata atttgtttgt gaataaatgg gctaacaggg
     2161 ctgagatttt taaacgatgt ttcaagtatt ttaattgtgt cattgtcaac tccagtgtta
     2221 agcaaaaatt cttcaaaatc aagcgggaac atttttaatt gaaacatata tccaacagga
     2281 accgatttta catttgccag ttcaagtccc aaaagcgagc cgctaaaaat gtagcgaaat
     2341 gaaccttcct cgacaagaaa ttttactttc gtaattagct cgcagcactg ttgaacttcg
     2401 tcaataaata ttattgtttc ttttttcttg agaccatgat tagtgtacag ttttaattga
     2461 gcgataaatt ccgcggttga aatattttcg atgttttcaa gcatctcaac aaggtcttta
     2521 ttttctagca aatttaattc aatataatcg atattttcgt cgtttaacac ctttctgatt
     2581 gttgttgttt tgccaacttg acgcgctcca tcaattaata aagatgcttt attgcctatc
     2641 aatcattcct tgattttatg ttcaattttt ctatttaaca ttataagttc tacctcggct
     2701 tttagttata ttgtgtcact tttccaagaa aattatacac gattttgtca cttttccaag
     2761 taaaaattaa gcaattttgt cacttttccg agtagatgct gagttgacgc gaaaattatt
     2821 tggatagaac aatgtatttg ttgagcttat cggcagtgaa gctgtcaatt acgttgaact
     2881 tcgaagcgat ggcgtccact tcgtcgtttg tgaaattgtg gcagaagcgg aagacgtcat
     2941 ccctgtcttt ccagccgagg aagtagtccc ctgcctcgag gttttggaag ccgagtttat
     3001 cttgcgcaag ttttgttgtt tcttgagctt tattgaaaat tcgcgtatct ttttcgaatt
     3061 gccagaaaga cactattgcg accttgcttt tagttagctc tttaagaagt ttaatcctca
     3121 attcggcagt tgggatatgg tgcataacgc cgaagcaaac cgcaacatcg taagacttta
     3181 attctaattt gtcttctaat aatgaattaa caatatcaac ttgcttaact ttttcatctc
     3241 attcaaagtt gtcataatta tcaacttcta catttaaatt attttctttt agaaattttg
     3301 agaaacggca gttgccgcaa cccaaatcta aaactgaatt tccgttatga aaaaattgca
     3361 aacaattttt tcagccttcc cactcatgtt ggcgggtctc ggaaaacgat tcggcgactt
     3421 cggagtaaaa cttagttgta gttttattga tgagttttat ttcttcggcc gtgtaagaca
     3481 taggcgcttc gcgctattca tctctcatgt gagggaaaag taagacgtcg cggatagatt
     3541 cagaattcgt aagaagcatt acgaggcgat ctatgccaac tccaactcct ccagctggtg
     3601 gcatgccgta ttctaatgcg cgaacgtaat cagcgtcgaa gcccattgct tcgtcatctc
     3661 ccaaatcttt cgcagcaact tgtgccttga agcgctcggc ttggtcgact ggatcgttaa
     3721 gttcgctaaa tgcatttgca tattcatgac cgcaaataaa caattcaaaa cgctcggtat
     3781 aatttgggtc ttcgctcttt ttagcaagtg gagaaacctc tagcggatgg ccagtgacaa
     3841 aaattggttg aattaactta tcttcgcaag cagcctcaaa aatctctgcg ataagtttgc
     3901 ctttgcctca tcccttttgg gctttaccac catgcttctc caaaattttc ttgagttcat
     3961 cttcacttcg gtcaaatgaa atttcttcgc cagtggcctc agatgcaagt tctgtcattg
     4021 acttcgctgg ccactgccct gataaatcaa tttcttgacc ctgatattca actttaaggg
     4081 ttccacaagc agcctgtgcc gcagcttgga cgtagccttc agtcaggcgc atcatacctt
     4141 ccaaatctga aaacgcacaa taagcttcca tggttgtgaa ttctggattg tggtagtggt
     4201 ccatgccttc gtttctaaat tgacggccca tctcaaaaac gcgctcaaag ccaccaacca
     4261 acaaacgctt tagcggaagc tcggtcgcaa ttcgcaaaaa gtaatcgcgg tctagcgcat
     4321 tgaagtgagt tgtaaaaggc ttcgcattcg caccacccat aattgaatgt aaaaatgggg
     4381 tctctacctc gacatatcct tcgtcttcca taaaatggcg aatcgccgaa attatggcag
     4441 accttttttg gaagacttcc ttaacctcag gattagcaat caagtcaacg tagcgctgac
     4501 ggtagcgggt ctccaaatct gcaaggccgt gaactttttc aggaagcgga cgtaagctct
     4561 tagacagcag ttggcacttc tcaaccataa cagacagctg gccgcgcttg gtcttcattg
     4621 ccttgccggt aattccaacc cagtcgccaa cgtccaaatc cttagccttg gcataagcat
     4681 cttcgcctaa attatcaatg cggaagaaaa gctgcatgtc tcctgttgca tcgcgaagcc
     4741 ccataaacgt gactttacct tggcctcgct ttgaaagaat gcgcccggca accttaactt
     4801 cgtcatttgt
//
LOCUS       DBNCDC010000082         4796 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_11191_length_4796_cov_7.999578, whole genome shotgun sequence.
ACCESSION   DBNCDC010000082 DBNCDC010000000
VERSION     DBNCDC010000082.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11191_length_4796_cov_7.999578"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            233..394
                     /locus_tag="AC04VQ_06070"
     CDS             233..394
                     /locus_tag="AC04VQ_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553688.1"
                     /translation="MQEILEMLEQHDDYVCYVLDGQTVVLEDYLSVKPEDEARIKKLV
                     KADRLKIGP"
     gene            407..628
                     /locus_tag="AC04VQ_06075"
                     /pseudo
     CDS             407..628
                     /locus_tag="AC04VQ_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013256.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 N-terminal
                     domain-containing protein"
     gene            692..2707
                     /locus_tag="AC04VQ_06080"
     CDS             692..2707
                     /locus_tag="AC04VQ_06080"
                     /inference="COORDINATES: protein motif:HMM:NF019368.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HIO9553689.1"
                     /translation="MIPKDAEKIRERFDKALPLLEKYSTTGDLLVPNGHDQMPVQQDI
                     FEIMELMRKEYPDYEIKLGTYEDQFKNIDKKSITKITSELLDGKYQRIHRSIYSSRAD
                     LKSANTRIENKIANILEPIASICYSLGFDYQSGTIEQIWREMLKNHAHDSICCCNTDK
                     VNKQVENRFVTSEDMVDNLTEFYMRKILEAIPGDEDKLVVFNTLPQDRKESLKVNIST
                     PFDDFDLQDIDGNKIDYDIISKESSVVRDEAEDSLKNYSKYQISICDEIPALGYKVYK
                     ILKSDEKRNSIITSRDKSIENKFYKIHINNNGSLNIDDKKTGNTYHNVLLLEDSADGG
                     DSYDYSPIEDDWTLTSEDVSAEVQTMSGSSFEEAKINYRFKVPKNLESRRFKQADSYV
                     DVEIKVLLPLASSNIELEFNVDNQAKDHRLRFLVPQNTSAKNSVADIQFGKIKRAVYD
                     KAVDVWQEENWDERPDTIYPFLNYVHTDTQAGLAVLSNSVREYQIIDGQAPSDTIAIT
                     LFRSYGHQGRKDLLRRPGRASGTNTPTPDAQLIGINTYKLGLTSEIKDVAGLANAYTT
                     PFVYYAFDPAEDLLLNKSKVEVPEKYSLLHFEISGIILSALKKAESGKGLVVRMFNSS
                     SAPQKLNISGVRKIASELMLDETEKCKIKGDSQIEFKPFEIKTFMLN"
     gene            2722..2904
                     /locus_tag="AC04VQ_06085"
     CDS             2722..2904
                     /locus_tag="AC04VQ_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553690.1"
                     /translation="MLSKIQNFGSAMLLPVMFMSFSGIAIGLSVLFTSPALFGDWVAD
                     ENLFWYQFWNLISVGG"
     gene            2920..3750
                     /locus_tag="AC04VQ_06090"
                     /pseudo
     CDS             2920..3750
                     /locus_tag="AC04VQ_06090"
                     /inference="COORDINATES: protein motif:HMM:NF014437.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            3781..4314
                     /locus_tag="AC04VQ_06095"
     CDS             3781..4314
                     /locus_tag="AC04VQ_06095"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit EIIB"
                     /protein_id="HIO9553691.1"
                     /translation="MFELGACGQLSGSLFVFLSQYAPLFALHWKEILIVIVVGIAFVF
                     IYIFVFTAWIKKFDVPTPGRLDEAEMRLRTKAEYREQQHSKADEKAGKKKKAKDDTTL
                     VAAIEEGVGGRENIDYFTNCVSRLRITVKDKSKLKDDAYFKSIGTKGAVHVDKTVQII
                     IGTDVPQVRTAFEEYIE"
     gene            4390..4465
                     /locus_tag="AC04VQ_06100"
     tRNA            4390..4465
                     /locus_tag="AC04VQ_06100"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4423..4425,aa:Phe,seq:gaa)
     gene            complement(4591..>4796)
                     /gene="rpmB"
                     /locus_tag="AC04VQ_06105"
     CDS             complement(4591..>4796)
                     /gene="rpmB"
                     /locus_tag="AC04VQ_06105"
                     /inference="COORDINATES: protein motif:HMM:NF013026.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIO9553692.1"
                     /translation="KVRIMSKVCEICGKAPAAGRSVSHSHRVSNRTFAPNIQRVTIKD
                     SKGKVRKANVCTSCLKAGKVTKA"
BASE COUNT         1430 a          866 c          987 g         1513 t
ORIGIN      
        1 ttttttcgcc ttatctgtaa atttgtcaaa agatgccatt tctcaccttt caaaaatttt
       61 ggaccccata ttatagtaca tttgcccatc attagatatg ttataatctt tcctgctaaa
      121 tttcaatagg agatagatga ctaaaaagtc cataaatatt gtgcctcaca cccattggga
      181 tagggaatga tattttaccg acgcaaaatc taaaatttat cttgtaaatg atatgcaaga
      241 aattttggaa atgcttgaac aacatgatga ttacgtttgc tatgttcttg atggtcagac
      301 tgttgttttg gaagattatt tgtctgtcaa gcctgaagat gaagcaagaa tcaaaaaatt
      361 ggttaaagcg gatcgcctga aaatcgggcc gtgatacact caaactgacg aaatggttgt
      421 tgcgggtgag tcgattgtcc gtaatttgct gtacggaaaa ttggattgcg ataaattcgg
      481 cggcagcatg atgattggct atttgccaga ttcttttggt cagtctgcgg caatgcctaa
      541 aatcttaaat ggttttggaa tcgacaagtc tcttttctgg cgaggatatt cagagcgcct
      601 tggcattaat caaactgaat ttttgtgaga ggctgacgat ggttcgcaaa cgacagttca
      661 acttttacca attaactatt ctgttggcat catgattcca aaagacgctg aaaaaattcg
      721 cgagcgtttt gataaagcgc taccactttt ggaaaaatat tcaactacag gtgatttgct
      781 tgttcctaac ggacatgatc aaatgcctgt tcaacaagat attttcgaaa tcatggagct
      841 tatgcgtaag gaatatccag attatgaaat taaattgggc acatatgaag accaatttaa
      901 aaatatcgac aaaaagtcaa taacgaagat cacaagtgag cttttggatg gaaaatatca
      961 gcgaattcat cgaagcattt attcttcgcg tgcggatttg aaatctgcaa acactcgaat
     1021 tgaaaataaa attgcgaaca ttcttgaacc aatcgcaagc atttgttatt cgctcggttt
     1081 tgattatcaa agtggaacaa tcgaacaaat ttggcgagag atgcttaaaa atcatgctca
     1141 cgactcaatt tgttgttgca atactgataa ggttaacaag caggttgaga atcgcttcgt
     1201 cacttcggag gacatggttg ataatctaac tgagttttat atgcgtaaaa ttcttgaggc
     1261 aattccgggc gacgaagaca agcttgttgt gtttaacact ttgccgcaag atcggaaaga
     1321 aagcttgaag gttaatattt cgacgccttt tgatgatttc gatttgcaag atatcgacgg
     1381 aaacaaaatt gattacgaca ttattagtaa ggaatcgagc gttgttcgcg acgaagccga
     1441 agattcgctt aaaaattaca gcaagtacca aatttctatt tgcgatgaaa tacctgctct
     1501 tggctataaa gtatacaaga ttttgaagtc ggatgaaaag cgcaattcaa ttattacgtc
     1561 tcgcgacaaa agcattgaaa ataagtttta caaaattcat atcaacaaca atggaagctt
     1621 aaatattgac gacaaaaaaa ctggcaacac ctatcacaac gttttgcttc tcgaagattc
     1681 agcggatggt ggagacagtt atgattattc gccaatcgaa gatgactgga cccttacttc
     1741 ggaggatgta agtgccgaag ttcaaacaat gagcggttca agttttgaag aggcaaaaat
     1801 taattaccgt tttaaggttc caaaaaattt ggagtcgagg cgttttaagc aggctgattc
     1861 ttatgttgat gttgaaatta aggttttgct gccgcttgct tcttcaaata ttgagcttga
     1921 atttaacgtt gataaccagg caaaagacca tcgccttcgc tttttggttc cgcaaaacac
     1981 atcggcaaaa aattcggttg cggatatcca gtttggaaaa atcaagcgcg cagtttatga
     2041 caaggcagtt gatgtttggc aagaagaaaa ttgggatgag cgtccagaca caatctatcc
     2101 gtttttgaat tatgttcaca ctgatacgca agctggcttg gccgtgcttt caaattctgt
     2161 tcgcgaatat caaattatcg atgggcaggc accatccgac acaattgcta ttacattatt
     2221 tagaagttat ggccatcaag gacgcaaaga tttactccga agaccaggtc gagcctcggg
     2281 aacaaatact ccaaccccag atgctcaact cataggcatt aacacttaca agctgggctt
     2341 aacttcggag attaaagatg ttgccggact tgctaatgct tatacaactc catttgtcta
     2401 ttatgcgttt gatcctgccg aagacttatt gcttaacaaa tcgaaggttg aagttcctga
     2461 aaaatattcg ctattgcatt ttgaaatttc agggattata cttagcgctt tgaaaaaagc
     2521 ggagagcgga aaaggattag ttgtccgaat gtttaattct tcatctgctc cgcaaaaact
     2581 taacataagc ggagttcgca agattgcttc ggaacttatg ttagacgaaa cagaaaaatg
     2641 caaaattaaa ggcgattcac aaattgaatt taagcctttt gaaataaaga catttatgtt
     2701 gaattagttg aaaggaaaat tatgttgtcg aaaattcaaa attttggttc tgcaatgctt
     2761 ttgccagtta tgtttatgtc attttccggt attgcaattg gcttgtcagt tttgtttaca
     2821 agccctgcac tttttggaga ttgggtcgca gacgaaaact tgttctggta ccaattttgg
     2881 aacttaatta gtgttggtgg ttgaactgtt atcaagcaaa tccctcttgt ttttgccgtc
     2941 ggcttacctg ttggtttagc taaaaaagca aatgctcgtg catgcatcga ggtattaatc
     3001 acatttatca ccttccatta ttttgtaaac acaatgatta gcacatggcc ggaatttttc
     3061 cacaacattg atatgacagc agatgttgat acttcaatgc gtctaacaat ggtagctaat
     3121 attccaactc ttaacatggg tgtttttgga gcgcttctaa tttctggaat tactgtttgg
     3181 attcacaaca gattttatga taagaagttg cctgttgccg tatctgtttt tagcggttcg
     3241 gtatttgttt acacaattgc atttgttgta atgcttccag ttgctttctt agcttcgcta
     3301 atttggccat acattcaaga tggtattttt gctattggaa catttgtaaa cacatctggt
     3361 cctttcggca tgtgaattta tattttcttg gaaaaaggct tattgccatt tggtattcac
     3421 ggaatgcttt ggatgcctat ggaattcgac aacattgtta tggatggcgg aatcgatgca
     3481 aattggtttg ctgcacttcc tggtcttgca acagacccta cacctattaa ggaagttatt
     3541 ccagaggctc gtttcacaat gacaaatcca tctcgaatct ttggagcaac cggtatttgt
     3601 gttgctatgt atatttgtgc aaagccagaa aacagaaaga aagttttggg tattttaatc
     3661 cctgcttggg tttctgcagt attctgcgca atttcagaac ctcttgaatt tacgttcctc
     3721 tttattgctc ctgctttgtt ctatgttcat gcggccctgg cagcaaccat gtcaacgctt
     3781 atgtttgaac ttggtgcttg tggtcagtta agcggaagtt tgtttgtgtt cttgagtcaa
     3841 tacgctccgc tcttcgcttt gcattggaaa gaaattttga tagttattgt tgttggtata
     3901 gcttttgttt tcatttacat tttcgtattt acagcatgga tcaaaaagtt cgatgttcca
     3961 actccggggc gtcttgacga agccgagatg cgccttcgca ccaaagctga atatagagaa
     4021 caacagcaca gtaaggcaga cgaaaaagct ggtaagaaga aaaaagcaaa ggacgataca
     4081 accttagttg ctgctatcga agaaggtgtt ggcggtcgtg aaaatattga ctacttcaca
     4141 aattgtgttt cacgtttgcg tattacagtt aaggataaat cgaagcttaa agacgatgca
     4201 tactttaaga gcatcggaac aaaaggtgcg gtacatgttg ataaaacagt acaaattatt
     4261 attggtacag atgtacctca agttcgcacc gcctttgaag aatatatcga atgagagtcc
     4321 taaaatttag ggtttaactc ttattgttgt gctattatat gcactcgcct gttagggcat
     4381 ttgctggctg gtcgggtagc tcagttggta gagcagcgga ctgaaaatcc gcgtgccgag
     4441 ggttcaagtc cccccccgac caccatttaa aaaaatttgc cgaatggcaa atttttttat
     4501 tttatcgccg actttaacaa atcatcaatg taataaataa aaaagacgaa gccgaagcct
     4561 cgtctttttg tttaactatt tgtgtgagat ctaagctttt gtaactttac cagcttttaa
     4621 gcagctagtg caaacattag ctttacgaac tttaccttta gaatctttaa tggtaacacg
     4681 ttgaatattt ggtgcaaaag ttctattgct aacacgatgc gagtgactaa cactacgtcc
     4741 cgcagctgga gcttttccac atatttcaca tacttttgac ataattctca ccttct
//
LOCUS       DBNCDC010000083         4776 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_11271_length_4776_cov_6.218174, whole genome shotgun sequence.
ACCESSION   DBNCDC010000083 DBNCDC010000000
VERSION     DBNCDC010000083.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11271_length_4776_cov_6.218174"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /gene="purN"
                     /locus_tag="AC04VQ_06110"
     CDS             complement(<1..442)
                     /gene="purN"
                     /locus_tag="AC04VQ_06110"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIO9553693.1"
                     /translation="MLNKQANIGVLVSGGGTNLQALIDAQSSGIIKSGKISLVVSNNP
                     DAYALERAEKAGIKTAIMENEDQLIKMLDEANIDLIVLAGFLAILSKNFTDHFKDRII
                     NVHPALIPSFCGEGFYGLKVHEAALAKGVKVSGATVHFVNEIPDG"
     gene            complement(436..>1471)
                     /gene="purM"
                     /locus_tag="AC04VQ_06115"
     CDS             complement(436..>1471)
                     /gene="purM"
                     /locus_tag="AC04VQ_06115"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIO9553694.1"
                     /translation="NSKSDAYASAGVDITAGYKAVELMKQHIAKTMTPGALSDIGGFG
                     GLFELDLNGIEKPVLVSGTDGVGTKLKLAFLLDKHNTIGIDCVAMCVNDIICCGARPL
                     YFLDYIACGKNIPEKIAQIVEGVAEGCVQSGAALIGGETAEMPGFYSEDEYDLAGYST
                     GVVDKTKIIDPNKMEEGDIIIALPSSGVHSNGFSLVRKVFDVENEDITSPVSELDGKS
                     IGETLLTPTKIYVKPVLQLIENVSVKAISHITGGGFYENIPRSIPDDFGAKIDKSAVR
                     VLPIFNLIAKRGNISEHDMFNTFNMGVGMSIVVPASEQDKALEILKANGEDAYVIGEI
                     VKSDEKIEIC"
     assembly_gap    1472..1481
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1559..2119)
                     /locus_tag="AC04VQ_06120"
     CDS             complement(1559..2119)
                     /locus_tag="AC04VQ_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553695.1"
                     /translation="MPDSGVPHAIGYAHKSKAPYARAFIKYTPSWSRSFMGSKQSERN
                     NVAKMKQIPVTELIKNKELLFVDDSIVRGTQLQKTVNFLYENGAKGVHMRSACPPIMF
                     GCKYLNFTRQTSDMELLTRKTILKLEGKKGLEHIDEYTDGSTKRGKKLRKTICDDLGF
                     KSLKFQSLDNVIKAIGLKPCDLCTYC"
     gene            complement(2252..2968)
                     /locus_tag="AC04VQ_06125"
     CDS             complement(2252..2968)
                     /locus_tag="AC04VQ_06125"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF024927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIO9553696.1"
                     /translation="MGGFFGITSRRACIADVYFGADYHTHLGTHSGGMAAYDKKIGLQ
                     RKIRHIDSAPFRTQLADCLESMHGNAAIGCINDNEPQPMLIRSKHGAYAISFVGKIIN
                     KDEIIKKYLSSVGGHFDAMTGGNVNSCELVAALIDQKDGLTEGITFAQDLIEGTASIL
                     VLTDKGALYAARDKFGRLPVQIGKNGKGHAVSLESFPLEKLDFEIIKELGPGEIVKLT
                     PGKMIQMKKPGKTKKICSFL"
     gene            complement(2984..3670)
                     /locus_tag="AC04VQ_06130"
     CDS             complement(2984..3670)
                     /locus_tag="AC04VQ_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIO9553697.1"
                     /translation="MSEELIYSGKTKDVFKMENGNVRLKFKDDCTGKDGKFDPGENSI
                     GLTIEGVGDVNLRMSIYFFEKINAAGIKTHYVSANLDDTTMEVLPAKVFGKGLEVICR
                     YRAVGSFYKRYGDYIEEGAELPAYVEMTFKDDDKGDPLVTKDGLIVLGTMNDKQYEDI
                     KKMTQDITKIVADDLKEKGLDLYDIKFEFGYDENGDVMLIDEIASGNMRVYKDGEYIE
                     PMKLSELFFA"
     gene            complement(3681..4163)
                     /gene="purE"
                     /locus_tag="AC04VQ_06135"
     CDS             complement(3681..4163)
                     /gene="purE"
                     /locus_tag="AC04VQ_06135"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013978641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIO9553698.1"
                     /translation="MKKIGIVMGSDSDLPIVEKATKTLDELGIAYETHIYSAHRTPDE
                     AKEFAVNARKNDFGVLIGVAGMAAHLAGALAANTTLPVIGIPCKGPNLDGIDALLSTV
                     QMPSGVPVATVAIDGGKNAAILAAQILAVEDTELAAKLDKIKSENAKDVLAKDKKLSN
                     "
BASE COUNT         1298 a         1031 c          961 g         1476 t
ORIGIN      
        1 caccgtccgg aatttcattg acgaagtgaa ccgtcgcgcc actaactttg acgccttttg
       61 cgagcgcggc ttcgtgaacc ttgagtccgt agaaaccttc gccacaaaac gaaggaatga
      121 gtgccgggtg gacgttaatg attctgtcct tgaaatgatc agtgaaattt tttgacagga
      181 ttgccaaaaa accggccaac acaataaggt cgatattggc ttcgtcgagc attttaatta
      241 gctggtcttc gttttccata atggctgttt tgattccagc tttttcggcg cgctctaagg
      301 cataagcatc tggattattt gaaacaacga ggctaatttt cccgctttta ataattccag
      361 aactttgtgc gtcaattagg gcttgtaaat ttgtgccgcc gcctgaaacc aaaactccta
      421 tgtttgcttg cttatttaac atatctcaat cttttcatcc gactttacaa tttcgccaat
      481 gacataagcg tcttcgccgt ttgctttcaa aatttcaagc gctttatctt gttcgctagc
      541 aggaacaaca atgctcatgc caacgcccat attaaatgtg ttaaacatat cgtgttcaga
      601 gatgtttcca cgcttggcaa ttaagttaaa aattgggaga acgcgaacag cactcttatc
      661 aatttttgca ccgaaatcat cggggatgct tcgtggaatg ttttcgtaaa atccaccgcc
      721 agtaatgtga gagatggctt taacgctaac attttcaatg agttgcaaaa caggtttgac
      781 ataaattttt gttggagtca aaagagtttc tccaattgat tttccgtcaa gttctgaaac
      841 tggcgaagtt atgtcttcgt tttcaacatc gaaaactttt ctaacaagac taaagccgtt
      901 tgaatgaact ccacttgatg ggagagcgat gataatatcg ccttcttcca ttttatttgg
      961 atcgatgatt ttagttttgt caactacacc agttgaatag ccagcaaggt cgtattcgtc
     1021 ttcgctgtaa aaaccaggca tctcggctgt ctcgccacca attagagcag cgccggattg
     1081 gacgcagccc tcggcgacgc cttcgacaat ttgagcaatc ttctcaggaa tatttttacc
     1141 acacgcgatg taatccaaaa aataaagtgg acgagcgcca caacaaataa tgtcattgac
     1201 acacattgca acgcagtcaa ttccaattgt gttgtgcttg tcaagcaaaa atgcaagctt
     1261 aagtttagtt ccaacaccat ctgtgccaga gaccaaaacg ggtttttcga tgccatttaa
     1321 atcaagctcg aaaagacctc cgaagccccc gatatcagac aaagcgccag gtgtcatggt
     1381 ctttgcaatg tgctgtttca tgagctcaac agccttatat cctgcggtaa tatctacacc
     1441 agcgcttgcg taagcatcgc ttttagaatt annnnnnnnn nccattcacc aatttgcctt
     1501 ggcagaattg tactgccttg gctaaatcgg tgctagagaa ttcatattcc tttccgtttc
     1561 agcaatatgt acaaaggtca caaggtttaa gaccaatggc tttaattaca ttatcgagcg
     1621 attggaattt caaagattta aatccaaggt catcgcaaat tgtcttgcga agttttttgc
     1681 ctcgcttggt tgagccatct gtatattcat cgatgtgttc caaacctttt ttgccttcga
     1741 gcttaagaat tgtttttctt gtgagaagtt ccatatcgct tgtttgtcgc gtgaaattta
     1801 aatacttgca cccaaacata attggagggc aggcagatct catgtgaaca cccttcgcac
     1861 cattttcgta taagaagtta acggtctttt gaagttgagt tccgcgaaca attgaatcat
     1921 caacgaagag aagttccttg ttcttgatta actctgtaac agggatttgt ttcatcttcg
     1981 ctacgttgtt tctttcgctt tgctttgaac ccataaagct tcgagaccaa cttggagtgt
     2041 atttaatgaa ggcacgagcg taaggcgcct ttgatttgtg agcgtaacca attgcatgag
     2101 gcacacccga atctggcacg ccgccaacat aatcaaggtt tttgattcac ttttggtttt
     2161 tatctctatc cgctaacaaa gaaccgtttt tgtagcgcat catctcgacg tttgttcctt
     2221 cgtaagtgct ggtagggtag ccataataac ttcaaaggaa agagcaaatc ttcttcgttt
     2281 tgccgggttt tttcatttga atcattttac ctggagtaag ttttacaatt tctccggggc
     2341 caagttcttt gataatttca aagtcaagtt tttctaatgg gaaggattcg agcgagactg
     2401 catggccctt tccgttctta ccaatttgaa cggggaggcg accaaactta tctctagctg
     2461 cgtaaagtgc acctttgtcg gttagcacaa gaatagaagc tgtgccttcg attaggtctt
     2521 gcgcgaaagt tatgccttcg gtaagtccat ctttttgatc gataagcgcg gcaacaagtt
     2581 cgcatgaatt gacgtttccg cctgtcatcg catcgaagtg accgccaacc gagcttaaat
     2641 attttttgat gatttcatcc ttgttgataa ttttgccgac aaaacttatg gcataagccc
     2701 catgttttga acggatcaac attggttgag gttcgttgtc attaatgcaa ccgatcgcgg
     2761 catttccgtg catcgattca agacagtccg caagttgagt cctaaacggg gcgctgtcga
     2821 tgtgacgaat ctttcgctga agaccaattt ttttgtcata tgcagccatt ccgccactgt
     2881 gtgttcccag gtgagtgtga tagtctgcgc caaaataaac gtctgctata caagcacgtc
     2941 ttgaagtaat tccaaaaaat ccacccatta catctcctaa agtttacgca aagaacaatt
     3001 ccgaaagttt cattggctcg atgtattcgc catctttgta aacacgcatg ttgcccgaag
     3061 caatttcgtc aatgagcata acatcgccat tttcatcgta gccaaattca aatttaatat
     3121 cgtaaaggtc aaggcctttt tccttcaagt catcagcaac aatttttgtg atgtcctgag
     3181 tcatcttttt gatgtcttcg tattgcttgt cattcatagt gccaagaaca atcaatccgt
     3241 cttttgttac aagcggatcg cccttgtcgt cgtccttgaa agtcatctca acgtatgctg
     3301 ggagttcggc gccttcttcg atgtaatcgc catagcgttt atagaaactt ccaacggcac
     3361 ggtaacgaca aattacctcg aggccttttc cgaatacctt agcaggtaaa acttccatgg
     3421 ttgtgtcgtc aagattagca ctaacgtagt gagttttgat gcccgctgca ttgatttttt
     3481 cgaagaagta gattgacata cgtaaattaa cgtctccaac cccttcgatg gtaaggccaa
     3541 ttgaattctc gccaggatca aatttaccgt cttttccggt gcaatcatcc ttgaatttaa
     3601 gacgaacatt gccgttttcc atcttaaaaa cgtcttttgt ttttccagaa taaattaact
     3661 cttcgctcat gatttctcct ttaatttgaa agttttttat ctttagcgag gacgtcttta
     3721 gcgttctcac tttttatctt gtctaatttt gcagcaagtt ctgtgtcttc gacagcaagt
     3781 atttgagctg ccaaaatagc ggcattcttg ccaccatcaa tcgcaactgt tgcaacaggg
     3841 actccgctag gcatttgaac cgttgataaa agcgcatcaa ttccgtctaa atttggtcct
     3901 ttacatggga ttccaataac tggaagggta gtgtttgctg caagggctcc tgccaggtgg
     3961 gctgccattc ctgctacacc aattaaaaca ccgaagtcat tttttcgagc gttaacagca
     4021 aattccttgg cttcgtcagg agttctgtgt gccgaataaa tgtgagtttc ataggcaatt
     4081 ccaagctcat ctaaagtttt tgttgccttt tcaacaattg gaagatcgct gtcacttccc
     4141 ataacaatgc caattttttt catacttcct ccttgtagac aataaaaaaa gcaccctcta
     4201 ttttgggtgc ttatgtaggg ctactggagc ctgttgtttg caacgctttg tgcaaaggct
     4261 gccttactca ttgtctccgt ataaaatttt agtgtttaac atagcaaaac cgattttagc
     4321 aaatgtggtg taaactgtgc cgaaaacttt gcgaaaaaca caaaattatg tatttttcca
     4381 tttgcctgcg ggcgaatcga gtaaatgcca cgagtaaatc gagtgctggc cccggggaaa
     4441 tcgagtgtgg gccccgggga aatcaaaata aaaaaagttg agattttcct tgacttttct
     4501 gcagttttgt agtttaattt catctttggt gcgcaaatcg caggggagca aaaattcggt
     4561 tacgaaggcc tctgcaaaag cgcaggtaaa aatagataaa atttggagtt caatatagta
     4621 gaaaggtggc tgtttttatg gcagacaaaa aatcagcgaa aacttcaaaa gcttctcagg
     4681 ttgttaaacc aaagaaaacc tcaaaaaaca acatccgaac acgtaaaagt tttgcaaaaa
     4741 ttccagaagt tatggaaatg ccaaacttga ttggtg
//
LOCUS       DBNCDC010000084         4688 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_11639_length_4688_cov_6.830995, whole genome shotgun sequence.
ACCESSION   DBNCDC010000084 DBNCDC010000000
VERSION     DBNCDC010000084.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11639_length_4688_cov_6.830995"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(128..427)
                     /locus_tag="AC04VQ_06140"
     CDS             complement(128..427)
                     /locus_tag="AC04VQ_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553699.1"
                     /translation="MKGIIMELATKKGIPDSLNAGNMSKEDFELMIEESLKDIENNIV
                     YSLDELRSNIDLDFMKPNKETLKAMKEIDDMEVHPEMHKGNIDINKMFDDILEDS"
     gene            complement(778..853)
                     /locus_tag="AC04VQ_06145"
     tRNA            complement(778..853)
                     /locus_tag="AC04VQ_06145"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(818..820),aa:Arg,seq:cct)
     gene            complement(923..2857)
                     /gene="pknB"
                     /locus_tag="AC04VQ_06150"
     CDS             complement(923..2857)
                     /gene="pknB"
                     /locus_tag="AC04VQ_06150"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HIO9553700.1"
                     /translation="MIGRVFSGRYKLVERIGTGGMAEVYRAEDTVLGRVVAVKVMLPQ
                     YAADKEFTDRFRQEAASAANLQNPYIVNIYDWGQDGEDYFIVMEYVRGSDLKSGIKAK
                     GAIGQRKVAEIASQVCQALATAHAQDIMHRDIKPQNIMVQPDGNAKVMDFGIARAKNS
                     VKTHTSSVLGTAHYISPEQAQGKEITQSSDIYSLGCVMYEAVTGKLPFDGEDAVAVAM
                     KQVNEKPAAPRSIKPDIDPDLEKIIGMAMNKDPQKRFPTARDMQNALQDYLAGRPVRF
                     GSSADKTAVIAGIGGAAAGVAAGAAMAGAAYGDSPDKTGVMSPEEVQQRAQKNYRADN
                     TGKKPKKPQKTEAEKKAAKKKRLIIFAIIAGILAVAAIFVFVVMKGVPNVVGKHVNDA
                     KTEITQANFEVGDITEEFNPDVEKDHVISQNPGWRAFDHAKINLVVSKGAEMVDVPDV
                     KKKSRSEATDILERAGFKVELTEDAYDKDVEAGQVCKQDPDGNTKAPKGSTVKITVSK
                     GVEKGDVPNVVNFSETDAVAALKAAGFEVSIKREYSDSVDEGFVMAQSPSGGQKADKG
                     SSVTITVSRGSGQVTVPNVVGMDYDSAKSTLQSAGLNVATSGGGNKVISQNPVSGNKV
                     AQGTTVTLTLGSNEAITVLN"
     gene            complement(2907..>4688)
                     /locus_tag="AC04VQ_06155"
     CDS             complement(2907..>4688)
                     /locus_tag="AC04VQ_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015761514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIO9553701.1"
                     /translation="ICEELGLLGGAAVLLLFLCFAIRGFLSAARAKTDVSSFVAVGLT
                     TTIVLQAFIIVGGVTKLIPLTGLTLPFISQGGSSLLASFIIVGFLLRCGDEGTGHEND
                     LKANEILFSNGVLGRVSLGKRLTGTLIVFSVLFAILVANLTYIMVIQADEIKHMPGNN
                     HVLIKEQYNKRGNIETEDGKVLAKSEKQEDGTYKRVYPENEFAAHLIGYASTKYGTSG
                     IESHCNDTLKGEQNFATWQDVLNNMMGKTEQGNDVTLTIDSDVQEAAEKAIAGRKGAC
                     VIVDSSNGAVKALASAPTYNNNDIESLLADQSGDSSAMFNRASQALYAPGSTFKILSL
                     ASSFMENVCKEDDVFDAPGTLDFYGGKVTNYAKTEYGRITLSRATEVSSNTVYAQVGE
                     KLTADKLVKNAENFMFNKKINFDIPLSEGLMPDPDEMTMWETGWAAAGVPVGEHNSPA
                     GPQTTVLQMALVGCAIANHGVINQPYLVKQIKNPSGEVSYTADTSELAKPVTPEVAAR
                     VASVLEGVVKRGTGTPAAISGITIAGKTGTAEKGGGRDDSWFVGFANDAQGGSVVIAI
                     VIEDSADAPSQANNVLKTALKKQGRLS"
BASE COUNT         1355 a         1085 c          860 g         1388 t
ORIGIN      
        1 taatttcgag gactgtttga gcactggcga cgccccgaaa accaaagttg taaatcttaa
       61 atcattgaaa attaagcgtt ccgtgcactg gcatcgtgtt cggagttcga acctccattg
      121 ctaaggttta ggaatcttct aaaatatcat caaacatttt attaatatct atattccctt
      181 tatgcatctc aggatgcact tccatatcat caatttcttt catagctttt aaagtttctt
      241 tattaggctt catgaaatca agatcaatat tgcttctcaa ttcatctaga gagtaaacta
      301 tattattttc aatatccttt aaagattctt ctatcattaa ttcaaaatct tcctttgaca
      361 tatttcccgc atttaaagaa tctggaattc cctttttagt agctagctcc ataataatgc
      421 ctttcacgat tttaacgtaa tgaatattct tcattgtata acaaatgtat tgctaaaagc
      481 aagcataaat tatttgtttt agcgaaggtg tggccagtgc gcttcggggg tggtactgga
      541 aactatagag aattctattc agtgatttta ttttatttta tttacgaaaa cacatttact
      601 ttatttactt aagaattggc aaagacagcg agaaataatt atatccttca gatgtgcccg
      661 cctttttgac gacgtgtact acgtacacga gtcaaaaaac aagggtgcag atgatggata
      721 gaattatttc gcagcgaaag cttaataaat ttgctgttaa gcaaatttgt gtagtgctgg
      781 cgtccctgag aggagtcgaa cctccgacgc acggtttagg aaaccgacgc tctatccact
      841 gagctacagg gacgcattaa aactagagaa tgccaaaaat cctgcagaaa aatgaaatag
      901 cttgattaaa tacaggaatg tttcaattca aaacagttat tgcttcattt gaaccaagtg
      961 ttaacgttac agttgttcct tgagctactt tatttcctga aactggattt tggctaatga
     1021 ccttgttgcc accgccagaa gtagctacat ttaaaccagc tgattgtaat gtagatttag
     1081 ctgaatcata atccattcca acaacgttag gaacagtaac ttgacctgag cctctgctaa
     1141 ctgtaattgt tacagaagaa cccttatcgg ctttttgtcc accacttgga ctttgcgcca
     1201 taacgaagcc ttcgtcaaca ctatcactat attcacgttt aatagaaact tcaaatcctg
     1261 cagctttaag tgcggcaaca gcatctgttt cagagaagtt aacaacgttt ggaacatcac
     1321 ctttttcaac acccttgctt actgtaattt ttacagttga accttttgga gctttagtat
     1381 tgccatcagg atcttgtttg catacctgac ctgcttctac atctttatca tatgcgtctt
     1441 cggtaagttc aactttaaag ccagcacgct caagaatatc agtagcttca gatcttgatt
     1501 ttttcttaac gtctggaaca tcaaccatct cggcgccttt agaaacaacc aagttaatct
     1561 ttgcatgatc gaaggctctc caacctgggt tttgactaat aacatgatcc ttttcgacat
     1621 caggattaaa ttcttcggta atgtctccta cttcaaaatt agcttgggtg atttcagttt
     1681 tagcatcatt aacatgcttg ccaacaacgt ttggaacacc cttcataaca acgaagacaa
     1741 agattgccgc aacagccaaa attcctgcaa taatagcgaa gataattaga cgcttctttt
     1801 tagcagcctt cttttcagct tcggtctttt gaggtttctt cggttttttg ccagtattgt
     1861 cagcacgata attcttctgt gcacgctgtt gaacttcttc aggagacata actccagttt
     1921 tatcaggaga gtcgccataa gcagcgccgg ccatagcagc cccagcagca acacctgcag
     1981 cagctcctcc aatgcctgca attacagcag ttttatcggc acttgagcca aagcgaacag
     2041 gacgtcccgc caaataatct tgcaatgcat tttgcatatc acgagcagtt gggaaacgct
     2101 tttgcggatc cttattcatt gccataccaa taatcttttc caaatctgga tcgatatctg
     2161 gcttgataga acgaggcgca gcaggctttt cgttaacctg tttcattgca acagcaacgg
     2221 catcttcgcc atcaaaagga agcttgccag ttacagcctc atacattaca caaccaagag
     2281 aataaatatc gcttgactga gttatctctt ttccctgagc ttgttcagga gaaatataat
     2341 gtgcagttcc aagaacagaa gaagtgtgag tctttacaga atttttagca cgagcaatgc
     2401 caaaatccat gactttagca tttccatcag gctgaaccat gatattttgt ggcttaatat
     2461 cacgatgcat gatgtcttgt gcatgtgcag ttgcaagcgc ttgacaaact tggcttgcaa
     2521 tttcggcaac cttacgctgg ccaattgcac ctttagcctt aattccagat ttaagatcag
     2581 agccgcgtac atattccata acaatgaaat aatcttcgcc atcttgaccc caatcataaa
     2641 tgttaacaat ataaggattt tgcaaattag cagcacttgc tgcctcttga cggaagcgat
     2701 cggtgaattc tttgtccgcc gcatactgag gaagcataac ctttacagca acaacgcgac
     2761 ccaagacggt gtcttcggca cgataaactt cagccattcc cccagttcca atgcgctcaa
     2821 cgagcttata acgcccgcta aacactcttc ctatcatatc tgtagccaca attttctcct
     2881 tctctagtcc tgcattatat aataaattaa cttaaccgcc cttgtttttt tagtgcggtt
     2941 ttcaaaacat tattagcttg cgaaggcgcg tcagcactgt cttcaataac aatggcaatt
     3001 acaacgctgc ctccctgcgc atcattagcg aagccgacaa accagctatc atctcgtccg
     3061 ccaccttttt cagctgtacc agttttacct gcgatggtaa ttccagaaat tgcagccgga
     3121 gtaccagtgc ctcgcttaac aacaccttcc aaaacagatg caacacgagc tgcaacttcg
     3181 ggagtgacag gctttgcaag ttcagatgta tcagcagtgt aagatacttc gccacttgga
     3241 tttttaattt gcttaaccaa ataaggctgg ttgattactc catgattagc aattgcgcaa
     3301 ccaacaagcg ccatttgaag gacagtagtt tgaggtccag ccggactatt atgctcacca
     3361 acaggcactc ctgcagctgc ccaaccagtt tcccacattg tcatttcatc aggatctggc
     3421 attaggcctt cggagagcgg aatgtcaaaa ttaattttct tgttaaacat aaagttttcg
     3481 gcattcttaa caagtttatc ggctgttagc ttttcgccaa cttgagcata aaccgtgttc
     3541 gaagatactt ctgtagcgcg cgaaagagta attcggccat attcagtttt tgcataattt
     3601 gtaaccttac cgccataaaa atcaagcgtt ccaggagcat caaaaacatc atcttccttg
     3661 caaacatttt ccataaaaga gctggccaaa ctcaggattt tgaaagttga accaggagca
     3721 taaagagctt gcgatgccct gttaaacata gcacttgaat ctccgctttg atcagccagc
     3781 agcgattcga tatcattatt attatatgta ggcgccgaag ccaaggcctt aaccgcacca
     3841 ttgctcgaat ccacaattac gcacgcacct ttgcgccccg cgattgcttt ttcggcggct
     3901 tcttggacat cggaatcaat agtgagcgtt acatcgttgc cctgctcagt cttgcccatc
     3961 atattattta gcacgtcttg ccacgtggcg aagttctgtt cgccctttaa ggtatcattg
     4021 caatgcgact caataccaga agttccgtat ttagtcgaag catatccaat caagtgagcg
     4081 gcaaattcgt tttctggata aactcgtttg taggttccat cttcttgctt ttcgctcttg
     4141 gcgaggacct tgccgtcttc ggtttcgatg ttgcctcgct tgttgtattg ttctttaata
     4201 agaacatgat tattgcctgg catatgtttg atttcatccg cttgaataac cataatatat
     4261 gtaaggtttg cgaccaaaat tgcaaacaaa acgctaaaga cgatcaaagt tccagttagg
     4321 cgcttaccca aactaacgcg tcccaaaact ccgttgctaa acaaaatctc gttggctttc
     4381 aaatcatttt cgtggcccgt gccttcgtca ccacaacgaa gcaaaaatcc cacaatgata
     4441 aaacttgcca gaagcgatga accaccctga cttataaatg gaagagtaag accagtcagc
     4501 ggaatgagct tcgtcacacc tccaacaatg atgaaagctt gaagaacaat tgtggttgta
     4561 agaccaacag caacaaacga acttacatca gttttagcac gagcagctga taggaaacct
     4621 cgaattgcaa agcacaaaaa gagaagcaaa acggccgctc cgcctaaaag tccaagctct
     4681 tcacaaat
//
LOCUS       DBNCDC010000085         4664 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_11735_length_4664_cov_5.482751, whole genome shotgun sequence.
ACCESSION   DBNCDC010000085 DBNCDC010000000
VERSION     DBNCDC010000085.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11735_length_4664_cov_5.482751"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            59..1441
                     /locus_tag="AC04VQ_06160"
                     /pseudo
     CDS             59..1441
                     /locus_tag="AC04VQ_06160"
                     /inference="COORDINATES: protein motif:HMM:NF036635.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1451..1693
                     /locus_tag="AC04VQ_06165"
     CDS             1451..1693
                     /locus_tag="AC04VQ_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553702.1"
                     /translation="MKDLLTFKNISRFIIFNLIGILFFFVSIPIEFSIGDFSYSATTL
                     PIDHTVSLIRSIPYFDNVYAPIIIVIGAILPFIRKT"
     gene            1751..1951
                     /locus_tag="AC04VQ_06170"
     CDS             1751..1951
                     /locus_tag="AC04VQ_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553703.1"
                     /translation="MAIWIFKPDFIPEVWFRDDMMPFIYNKVVLPVSTLVPIGAIFLA
                     FLIGYGLMEFIGIFLRPIMRPI"
     gene            1982..2728
                     /locus_tag="AC04VQ_06175"
     CDS             1982..2728
                     /locus_tag="AC04VQ_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HIO9553704.1"
                     /translation="MASFVGSYSVGLLITNRVYKEGKYTTKEACIIATGFSTVSAAFL
                     VVVAKSLGVADGHQYLMENWNLYFFATFIITFIVTAITTRFFPLRKKSTKGYKDVKVP
                     EEPKIKLSQIPKVAINEGLTACSKGDSLGKGLKKNFIDGLNMALAIAPSIMSIGFLGL
                     LIAEYTPLFTWMGYIFAPFTWALGFGDDMFVMGTSCSISLAEMFLPASIVSGASMYAQ
                     ITSAIVCVSEILFFSASIPCILGTDIDIKL"
     gene            2870..3928
                     /gene="pheS"
                     /locus_tag="AC04VQ_06180"
     CDS             2870..3928
                     /gene="pheS"
                     /locus_tag="AC04VQ_06180"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013979311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIO9553705.1"
                     /translation="MALVDDLKNLKEKTHAAIEEAKNSEELDRVRVAVLGKQGELTGY
                     LRNMGSVAKEERAKVGKAVNEARQAVEGALEQKMSELKSAEMAAAIDASAVDVTLPGR
                     KQVVGTRHIINKITDEIAEVFLGLGYTVAEGTEVETDYYNFEALNAPKDHPSRGLQDT
                     FYVHDNSIDKPDQKMSDVLLRTQTSGVQVHTMEKGELPIYIIAPGKVYRRDVADPSHL
                     PQFTQIEGLVVDEGITFGDLKGTLEFFCKKVFGEDRKTRFRAHYFPFTEPSAEVDVSC
                     GICGGKGCRFCKGTGWSEILGCGMVDPNVLRYCNIDPEKYSGFAFGVGVERVACLKYN
                     VPDLRMLLQGDMRFLTQF"
     gene            3979..>4664
                     /gene="ytpR"
                     /locus_tag="AC04VQ_06185"
     CDS             3979..>4664
                     /gene="ytpR"
                     /locus_tag="AC04VQ_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012797635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HIO9553706.1"
                     /translation="MPDLPGNLQAFCDKLDLTGTGVEEVAVAGEGFDNIVTGQILSKE
                     KHPDSDHMWVTKVDVGAEKLQIVCGAQNFEEGDHVVVAKIGAVLPGDFKIKKAKLRGV
                     ESCGMNCSERELGLSDSHEGIMILPKDAPLGVPIADYLNLSDTVIDTEITPNRPDCLS
                     VVGCAREIAAIYDVKYKNPLKDMADKLKKCEFEKASMPTVKICEDERCPRYTARVIKG
                     VKVGPSPKWMV"
BASE COUNT         1217 a          882 c         1168 g         1397 t
ORIGIN      
        1 taaccccgct gctgctattg gtgttgcaaa ttatggacag atgaccgctg gtggctggat
       61 gtatattggg ccacagggaa ttgttcacgg cactttcaat acgttgctga acgccggtcg
      121 cttaaagctc ggactttctg agaaggaaaa cttggctggc aagctattta tttcttcggg
      181 tcttggtggc atgagcggcg ctcaaggaaa ggcagctcat attgctggag ctacagcgat
      241 catcgccgaa gttgacagtt ctcgaattca aactcgcttt gagcaaggtt gggtagatgt
      301 tgttgccgag ggcgccgaag aggcctgcaa gcttgcactc gagaagcaaa aggtggcgca
      361 agcttgcgcg atcgccatgc atggaaacat tgttgatttg ctggaatatg ttttggaaaa
      421 tgacatccat gtggatttgc tttcagatca aacatcttgc cacgttccat atgatggagg
      481 ttattgccca cagggactga ccttcgaaga gcgcactgaa atgcttgcaa atgacaagaa
      541 aaagttttgt gagcttgttg acaagactct cgttaagcac tatcacttga tttcagattt
      601 gcacgagaag ggaacctact tcttcgatta tggaaacagt tttatgaagg ctgtttatga
      661 tgctggggat aagacaattt gtaaaaacgg caaagatgat ttggacggat ttattttccc
      721 gtcttatgtt gaagatattt tgggtccgca actttttgat tatggttatg gtccatttag
      781 atggtgctgt ttgtctcgaa atcacgaaga tttgattaag acggacaagg ctgcctatga
      841 ttatatcgat gctcacaaca ggcgttacca ggatgaagat aataaagttt gagttcgaga
      901 tgccgaagag aacaagcttg ttgttggaac cgaatgtaga attttatatc aagatgcaat
      961 gggcagaact aacattgcac ttaagtttaa tgaaatggtt cgcaatggtg aaattggtcc
     1021 tgtaatgctc ggccgcgatc atcatgacac gggtggcacg gattcgccgt ttagagaaac
     1081 ttcgaatatt aaagacggtt caaacattat ggctgatatg gcacaccagt gcttcgctgg
     1141 aaattctgcg cgaggcatga gcttggtcgc gcttcataat ggtggcggaa ctggaattgg
     1201 caaggccatt aacggtggct tcggtatggt acttgatggt agcgagcgtg tggacacaat
     1261 tcttaaagcc gcgatgccat ttgacacaat gattggtgtt tcgcgcaggt catgggctag
     1321 aaatgaaaac gccattggtg ttgctgcaga atataacgaa gccaataagg gcaaggatca
     1381 tattactctt ccatatattg ttgatgaaaa agttgtttcg caaattgaca aaatgtttta
     1441 aggagtgcgc atgaaggatt tattaacttt taaaaatatt tcaagattta ttattttcaa
     1501 cttaattggt attttgtttt tctttgtttc tattccaatt gagtttagta ttggcgattt
     1561 ttcttatagc gccacaacgc ttccgattga tcatacggtg tctcttattc gaagcattcc
     1621 atatttcgac aatgtttatg ctcctatcat tattgttatt ggagcgattt tgccattcat
     1681 tcgcaagact tgaaacaaag acaaggtcac aattgctttt tcgtttttaa aacttcttgc
     1741 aattcctttc ttggcaattt ggattttcaa gccagacttc attccggagg tttggtttag
     1801 agatgatatg atgccgttta tctataataa agtagtactt ccggtttcca cgctcgttcc
     1861 aattggtgcg attttcctcg catttttgat tggttatggc cttatggaat tcatcggaat
     1921 tttcttgcgt ccgattatgc gtccaatttg aaagactccg ggacgaagcg cagttgatgc
     1981 tgtggcttcg tttgtaggaa gttatagtgt tggactttta atcactaaca gagtttataa
     2041 ggaagggaag tacacaacca aggaagcttg cattatcgcg actggctttt cgaccgtttc
     2101 agctgcgttt ttggttgttg ttgcgaagag cctcggcgtt gccgatggtc accagtattt
     2161 gatggagaac tggaacctct acttcttcgc tacatttatt attacattta ttgttactgc
     2221 gatcaccacg cgctttttcc cgcttcgtaa gaaaagcacg aagggttata aagacgttaa
     2281 ggtccccgaa gagccgaaga tcaagctttc gcaaattcca aaagttgcta ttaatgaggg
     2341 cttgactgct tgttcgaagg gcgatagtct tggcaagggt ttgaagaaga actttattga
     2401 cggtttgaat atggcgcttg caattgcccc ttcaattatg tcgattggct tccttggact
     2461 tttgattgct gaatacacac cgctatttac ctggatggga tatattttcg caccatttac
     2521 atgggcactt ggatttggtg acgacatgtt tgtaatggga acatcgtgct caatttcttt
     2581 ggctgagatg tttttgccag caagtattgt ttcaggcgct tcgatgtatg cccaaattac
     2641 atctgcaatt gtttgcgtat ctgaaatctt gtttttctcg gcttcgattc cttgcatttt
     2701 aggaaccgac atcgacatca aactttgaga gattttggtg atttggcttg aaagaataat
     2761 tttatcttta gtcattgcag gtttggtagt tagattttta ttcccaatat ttggcattcc
     2821 tgtattttag taaggtataa tacgctgcta gttaattaga gaaggtagta tggctttagt
     2881 tgatgattta aagaatttaa aagagaagac acacgcggcc attgaggagg ctaaaaactc
     2941 cgaagaactg gaccgcgttc gtgttgctgt tttgggcaaa caaggcgagc taactggcta
     3001 tttgcgcaat atgggcagcg ttgctaagga agagcgcgcc aaagttggta aggcagttaa
     3061 cgaagcccgc caggcagttg aaggcgcgct tgagcaaaag atgagcgagc ttaaaagtgc
     3121 cgagatggct gctgcaatcg atgcttcggc ggttgatgtg acgcttccag gccgcaagca
     3181 ggttgtgggc acgcgccaca ttattaacaa gatcacagat gaaattgccg aggtcttttt
     3241 gggacttggc tacacggttg ccgaaggcac cgaggtcgag accgactatt ataacttcga
     3301 agccctaaac gcgccgaagg atcatccttc gcgtggactt caagatacat tttatgtaca
     3361 cgacaattcg atagataaac cggaccaaaa aatgagtgat gttttgcttc gcacacaaac
     3421 aagtggagtt caagttcaca caatggagaa gggcgagctt ccaatttaca tcatcgctcc
     3481 tggtaaagtt tatcgtcgcg atgttgctga tccttcgcat cttccccaat tcactcaaat
     3541 tgaaggtctt gttgtggacg agggaatcac cttcggtgat ttgaagggga cgcttgaatt
     3601 tttctgcaag aaagtttttg gcgaagaccg caagactcgc tttcgcgcgc attactttcc
     3661 attcaccgag ccaagtgccg aagtagatgt aagttgcggc atttgcggag gtaaaggttg
     3721 tcgtttttgc aaggggaccg gatggtctga aattctcgga tgcggcatgg ttgacccgaa
     3781 tgtgcttcgc tattgcaata ttgaccctga aaaatatagc ggctttgcat ttggcgttgg
     3841 cgtcgagcgt gttgcttgtc ttaaatataa tgtaccggat ttgcgtatgc tccttcaagg
     3901 tgacatgcga tttttgactc agttctaagg aggtgtggca tgaagatttc tcttaattga
     3961 ttaaacgaat atgtaaaagt gccagaccta cctggtaatt tgcaggcttt ttgtgacaag
     4021 ttggatttga ctggaactgg cgttgaagaa gttgcggtcg cgggcgaggg cttcgataac
     4081 atcgtaactg gacaaattct atcgaaagaa aagcacccag attcggacca tatgtgggtc
     4141 acgaaagttg atgtaggcgc agagaagctt caaattgttt gtggcgctca gaacttcgaa
     4201 gaaggcgacc acgttgttgt tgcgaagatt ggcgctgtac ttccaggtga ttttaagatt
     4261 aagaaggcaa agcttcgcgg tgttgagagc tgcggtatga attgcagcga gcgcgaactt
     4321 gggctgtccg acagccatga aggtattatg attttgccta aggatgcgcc tcttggtgtt
     4381 ccaattgccg attacctcaa tctttctgat accgtaattg acactgaaat tacgcctaac
     4441 aggcctgatt gcttgagtgt tgttggttgc gcgcgcgaga ttgcggcaat ctatgacgtc
     4501 aaatataaaa acccgcttaa ggacatggct gacaaactta aaaagtgcga atttgaaaaa
     4561 gccagcatgc caacggttaa aatttgcgaa gacgagcgat gccctcgtta tacagctcga
     4621 gtcattaaag gtgtcaaggt tggaccttcg cctaaatgga tggt
//
LOCUS       DBNCDC010000086         4646 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_11812_length_4646_cov_10.319538, whole genome shotgun
            sequence.
ACCESSION   DBNCDC010000086 DBNCDC010000000
VERSION     DBNCDC010000086.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11812_length_4646_cov_10.319538"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(99..386)
                     /gene="groES"
                     /locus_tag="AC04VQ_06190"
     CDS             complement(99..386)
                     /gene="groES"
                     /locus_tag="AC04VQ_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIO9553707.1"
                     /translation="MKLQPLNDRVIVKADEAEEKTAGGLILTSDSKEKPVTGKVVAVG
                     DGALDANGQKVPVPVKVGDKVLYGKFGGTEVTVDGEELLILRAEDIYAVFK"
     gene            complement(601..900)
                     /locus_tag="AC04VQ_06195"
     CDS             complement(601..900)
                     /locus_tag="AC04VQ_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIO9553708.1"
                     /translation="MEYNIDKNDANVNVTVSGRIDTTTAPQLEEAILPEIDGANEVVI
                     DFKNLEYISSAGLRLLLMLQKKMNAAGGTFKILNVNDFVMEVFEMTGFKDILTIA"
     gene            complement(1123..2349)
                     /gene="dnaB"
                     /locus_tag="AC04VQ_06200"
                     /pseudo
     CDS             complement(1123..2349)
                     /gene="dnaB"
                     /locus_tag="AC04VQ_06200"
                     /inference="COORDINATES: protein motif:HMM:NF015736.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
     gene            complement(2659..3399)
                     /gene="rplI"
                     /locus_tag="AC04VQ_06205"
     CDS             complement(2659..3399)
                     /gene="rplI"
                     /locus_tag="AC04VQ_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIO9553709.1"
                     /translation="MKVILLQEVKGRGGEGDVIEVKDGFANNYLIKEGMAVKATKGAL
                     KQLEERRANIEKREEVRLAEANELKEKINNLKFKVDVQIGDEGQLFGAVTSQMIADGI
                     KKNADVEIDKRRIDIRKAIKTVGLHKAEVVIYRDIKANIEVIVGDEDAEKFMEALKAK
                     KERKSGAKGGKKPAKKDAKKEENGDKKDEEKVEDKKEDKPEEKVEEPKEEVKEEKPEE
                     KTDEPKEEEEKPVEEKAEEPKEEGENKE"
     gene            complement(3410..3670)
                     /gene="rpsR"
                     /locus_tag="AC04VQ_06210"
     CDS             complement(3410..3670)
                     /gene="rpsR"
                     /locus_tag="AC04VQ_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIO9553710.1"
                     /translation="MRKIEKQPVQMDCPFCKEKVEYIDYKDIKLLRKFVTDRGKIKPR
                     RVTGLCAQHQKDIANAVKRARMMALMPFAVTVVSKRGDRRRR"
     gene            complement(3693..4196)
                     /locus_tag="AC04VQ_06215"
     CDS             complement(3693..4196)
                     /locus_tag="AC04VQ_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIO9553711.1"
                     /translation="MSINRVNVSGNLTRDPELRQTAGGMSVLSFGIAVNDRRKNSQTG
                     EWEDYPNFLDCTLFGARADGLSKILTKGSKVAIDGKLRWSQWERDGQKRSKVEIIVDS
                     LDFMSQRNGGQAPAVASAPAVAPVASPATETFGAGTGEAAPIASAPAPEPADAGVEEP
                     YGEDIPF"
     gene            complement(4249..4542)
                     /gene="rpsF"
                     /locus_tag="AC04VQ_06220"
     CDS             complement(4249..4542)
                     /gene="rpsF"
                     /locus_tag="AC04VQ_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIO9553712.1"
                     /translation="MKAYELLYFVVPTADDKPKKIAQERVESTIKKLKGKIDNIEDQG
                     RKALAYEVDGATDGDYMLVDFHCDPAGVAELNRVLRLTVEVQRHMIVARTDRD"
BASE COUNT         1225 a         1147 c          839 g         1435 t
ORIGIN      
        1 acctttacag cttcgctgag tttatgaaca ccctcagcca gggctttacg tgcatcttct
       61 tcaaatttaa tgtctttagc catgatgtct cctctctatt atttaaagac agcgtaaata
      121 tcttcggcgc gaaggatcaa aagttcttcg ccgtccactg tcacttcagt gcccccgaat
      181 tttccgtaaa ggactttgtc gccaaccttg actggaactg gcactttctg gccatttgca
      241 tccaaagcac catcgccaac agcaacaact ttacctgtaa caggcttttc tttgctgtcg
      301 gaagtcaaaa ttaaaccgcc ggcagtcttt tcttcggctt catcagcctt tacaattact
      361 ctgtcgttta atggttgtaa tttcataatc tacttccttt ccacattaaa aaatccgctt
      421 tcgcggacgc actatattac cacgttttca aaaaaaatca acccaaaggt gtaattattg
      481 cctggcatga aatgcaccaa aaggtgtaat tattgccagg catgaaatgc accttttagg
      541 cataaaaaaa caggcgcacg tttcgcgaaa cgcattgcac ctgttatttt caaaagcaaa
      601 ttaagcgatt gtaaggatgt ccttgaaacc ggtcatttca aaaacttcca ttacaaaatc
      661 gttaacgttc aaaatcttaa atgttccccc tgcagcattc attttctttt gcaacattag
      721 caaaaggcga agaccagcag atgagatgta ttcaaggttt ttgaaatcaa taacaacttc
      781 gtttgcaccg tcgatttcag gcaaaattgc ctcttcaagc tggggagcag tagtcgtgtc
      841 aatacgacca gagacggtaa cattaacgtt tgcatcattc ttgtcaatat tgtattccat
      901 gttttcccct tttcctcgtc cgcctattat agcattaaaa tgcgaaaatt attaattttt
      961 tggattttgt ggattttcgg tatccgtgac gaagttcgca taaccaccag ccttattcgg
     1021 gttgtaataa tccgaaaact tagtgaaatc tggctcaaag gccaaagtca acaatttggt
     1081 tgggccgttg cgatgcttcg ccacatcaat aacagcagtt cctcaatcag gacggccttc
     1141 cgcctcggct tcttcctggt tcatgcttcg atcgatgaac ataacaatgt cagcatcttg
     1201 ttcgatagca ccagattcac gaaggtccga aagctgcggg cgacctttaa ctcctttata
     1261 aacacgctgt tctacctggc gtgaaagctg cgaaagagca ataaccggga tgtccatttc
     1321 cttcgcaaga atctttagac ctcgcgaaat ttcggcaact tcaaccgcgc ggtttccatc
     1381 tcttcgcatt gttggagctt gcataagctg cagataatcc acaacaatca agccgttttt
     1441 cacatcgcga agttcacgcc ttgccttcgc gcgcaaatca aagattgaaa gacctggggt
     1501 gtcgtcaatg taaagcggca aattgctcaa acttcccgaa gccgacacaa tggcgtttca
     1561 atcctgacct gcaattcggc cagatctaac cttgcttaaa ttcagctgag cttccgtgca
     1621 taaaacacgt tggattagct gattagcacc catctccaaa gacatgaatg caaccctggt
     1681 gtcttgcttg gcggcgttga ccgctaagtt cagggcaaaa gaagttttgc ctacaccagg
     1741 tcgggcagca atgataatta aatcaccacc acgaagcccg ttaaacaagt tgtcaagatc
     1801 cttaaagcct gttggaacgc cagctagctt accctttcgt tgggacaatt cttgaagctc
     1861 cgcaaaagag tcggtcaata ggtcgttcgc acttacaaaa tccgaagaca acttcttttc
     1921 agtaacgttg aaaagcgtcg actcggcttc gccaattact tggctcaaat tgtccggagc
     1981 gtcatatgcc atggccgaga ttttggccga ggcgtagaca agatcccgaa gcacagaagt
     2041 gcgtttgaca atgtccgaat gcgtgcgcca gtttgttagc gacatctcat tcgatgccaa
     2101 gtccaacaaa taattgcgac caccaatggc ttctagctgg ccttttcctt gaagattttc
     2161 cgccaacgaa atctgatcaa catcaatgtg gcgcttatac aaatcgataa tcgcctcata
     2221 gattgtttga tgcgctggac ggaaaaaatc ttttggcgca agtttaggag caagctcttc
     2281 cacggcaacc ttgttcagca tgcacgcggc caaaacactt ttttccgcat caatattttg
     2341 cggaagcatt ttaccggtgt tgttctcgga aatttcgcca acaaaatctg tgttaagcga
     2401 gccttgaacg tttgacctcg gttgtgtgtt agcgttaatt tgcgccacaa cttctccttt
     2461 taacttgcga tgtttcgcac tttatcacaa gcaaaacata aggtcaagcc taattttcaa
     2521 ttatttttta tccccaagat ttccacaatg aaaatgtgcc aaaataaaaa actcatgccc
     2581 ttggacaagg aaacatgagt tttttaaatc agaaataaaa gttattaaca aattccacaa
     2641 atttttgcgg aagtcaattt attccttatt ttcgccttct tcttttggct cttcagcttt
     2701 ttcttctact ggcttctctt cttcttcttt aggttcatcg gttttttctt caggcttttc
     2761 ttcttttact tcttcctttg gctcttcaac tttttcttct ggcttatctt cctttttatc
     2821 ttctactttt tcttcgtctt ttttgtcgcc attttcttct ttcttagcat ccttcttagc
     2881 tggctttttg ccgccttttg cgccagattt cctttctttc ttagccttta gagcctccat
     2941 gaatttctcg gcatcttcat cgccaacaat aacttcaata ttagctttaa tgtcacgata
     3001 aattacaact tcagctttat gaagtccaac agttttaatt gctttgcgaa tatcaattct
     3061 gcgcttgtca atttcaacat cggcattttt cttgatacca tcagcaatca tctgcgaagt
     3121 tacagctccg aagagttggc cttcgtcgcc aatttgaaca tcaaccttaa atttaagatt
     3181 gttaattttt tctttaagct cattagcttc agcaaggcga acttcttcgc gcttttcaat
     3241 attagcacga cgctcttcaa gctgcttgag tgcgcctttt gtagctttaa cagccatgcc
     3301 ttctttaatt agatagttgt ttgcaaaacc atctttaacc tctatgacat caccttcgcc
     3361 gccgcggcct ttaacttctt gcaataaaat aactttcatc acacacctcc taacgacgac
     3421 gtctgtcacc acgtttgctt acaacagtaa ctgcaaaagg catcaatgcc atcatgcgag
     3481 cacgctttac agcattcgca atgtcttttt gatgctgagc gcaaaggcca gttacacgcc
     3541 ttggtttaat tttgccgcga tcggtgacaa acttacgcag caatttaatg tctttgtagt
     3601 cgatgtattc gactttttcc ttgcaaaatg gacagtccat ttgcacaggt tgcttctcga
     3661 tttttctcat agaaattcct cctaattcct aattaaaatg gaatatcctc gccatatggc
     3721 tcttcaacgc cagcgtctgc aggttctgga gcaggtgccg aagcaatagg tgcagcttcg
     3781 ccagttccag caccaaaagt ttctgtggca ggacttgcta caggtgcaac tgcaggtgca
     3841 gaagcaacag caggagcttg tccgccattt cgctgactca taaagtcaag gctatcaaca
     3901 ataatttcaa ctttactgcg cttttgacca tcacgctccc attggctcca acgaagtttg
     3961 ccatcaatag caactttaga accctttgtc aaaatctttg acaatccatc agcgcgtgcg
     4021 ccaaacaaag tgcagtctaa aaagttagga taatcctccc attcgccagt ttgcgaattc
     4081 ttacgtctgt catttacagc aattccaaaa ctcaaaactg acataccacc agcagtctga
     4141 cgaagttctg gatcgcgtgt taaattacca cttacattta ctctattaat actcatttta
     4201 atctctcttt cttaaatacg tgacgaaggc acatcctccg tctgtaaatt aatctctgtc
     4261 tgttcttgcc acaatcatgt gtctttgcac ttcgacagtt aagcgcaaaa ctctgttaag
     4321 ctcagcaaca cctgctggat cgcagtgaaa atctactaac atgtaatcac catcagtagc
     4381 accgtcaact tcataagcca aagccttacg accctgatct tcgatattgt cgattttacc
     4441 tttgagtttt ttgattgtag attctacacg ttcctgtgca atctttttag gtttgtcgtc
     4501 cgctgttgga acaacaaaat acagtaattc ataagccttc atctttcacc tcctctggac
     4561 ttgcggcttc gggcttggtt cttaggctat ctacccgaag caggatagcc cggatatagt
     4621 agcacaaatt actgcgagaa gcaacc
//
LOCUS       DBNCDC010000087         4624 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_11911_length_4624_cov_6.114029, whole genome shotgun sequence.
ACCESSION   DBNCDC010000087 DBNCDC010000000
VERSION     DBNCDC010000087.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11911_length_4624_cov_6.114029"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="AC04VQ_06225"
     CDS             complement(<1..550)
                     /locus_tag="AC04VQ_06225"
                     /inference="COORDINATES: protein motif:HMM:NF017508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIO9553713.1"
                     /translation="MKVKVENLKDNNVKVVVDFTKDEVNKAISDKYAELAKKYKFPGF
                     RAGKAPRPVVDNMFTKEGVLAQVTDDTINKNYPLSIDEANIFPVGQPDFGKEESKLVE
                     EGKAYSFSYKILVEPKFTLSSYDKVKISLPSAEPTEKDIDAQLDQFREHYFTYEDAGA
                     SEKAKKDSTVSLKIEAKDDAGKA"
     gene            696..854
                     /locus_tag="AC04VQ_06230"
     CDS             696..854
                     /locus_tag="AC04VQ_06230"
                     /inference="COORDINATES: protein motif:HMM:NF014047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HIO9553714.1"
                     /translation="MEKNTIHKINITSYSSEGEGIGRIDGMAVFVKGGMIGETLEVKI
                     TKVAKNCA"
     gene            948..1220
                     /locus_tag="AC04VQ_06235"
     CDS             948..1220
                     /locus_tag="AC04VQ_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553715.1"
                     /translation="MNYEEELQLKLRRVNDALQRIGKLDFKINKIHGADNILRYRNKA
                     TFPVGYDKNANFKIGIFQQRSHNIIDINECFIQSEEAEAIVGIIRK"
     gene            1221..1469
                     /locus_tag="AC04VQ_06240"
     CDS             1221..1469
                     /locus_tag="AC04VQ_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553716.1"
                     /translation="MLEHKIPAYNEEQQTGLIRHLFVRTNNKKEVLVCLVTTKKEIPH
                     LDTLITLFKNSTIDIVGLVQNIQDENTNVIFGKQFNTL"
     gene            1482..2042
                     /gene="rlmD"
                     /locus_tag="AC04VQ_06245"
     CDS             1482..2042
                     /gene="rlmD"
                     /locus_tag="AC04VQ_06245"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIO9553717.1"
                     /translation="MKDKILDLDFKLSVASFFQVNRAQAEKLYTIALDYANIKANETV
                     VDLYCGTGTITLLMSKKAKRAFGIEIIPEAILDAKQNAIINSIDNADFICADAKTAAE
                     DFANNKFNPDVICVDPPRKGLDISTISSIIKMSPKRIVYISCDPATLARDLQIFSKSN
                     YKIVKAEALDMFPRTPHIESVVLLTK"
     gene            2160..3428
                     /locus_tag="AC04VQ_06250"
                     /pseudo
     CDS             2160..3428
                     /locus_tag="AC04VQ_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012799737.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
     gene            3462..3824
                     /gene="tsaE"
                     /locus_tag="AC04VQ_06255"
     CDS             3462..3824
                     /gene="tsaE"
                     /locus_tag="AC04VQ_06255"
                     /inference="COORDINATES: protein motif:HMM:NF014426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIO9553718.1"
                     /translation="MDKVIVNTLNELDEFCKGFCQSLKPGDVICLTGDLGAGKTAFVQ
                     SVAKAFNITDQVQSPTFNILLQYKNDEVAINHFDLYRLEKAQELEDIGFYEAVESKAF
                     SFVEWAEKFKDQMPEDSI"
     gene            complement(3853..4500)
                     /locus_tag="AC04VQ_06260"
                     /pseudo
     CDS             complement(3853..4500)
                     /locus_tag="AC04VQ_06260"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            complement(4500..4622)
                     /locus_tag="AC04VQ_06265"
     CDS             complement(4500..4622)
                     /locus_tag="AC04VQ_06265"
                     /inference="COORDINATES: protein motif:HMM:NF015113.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HIO9553719.1"
                     /translation="MVLGKKSLENGNVELKIRKSNERSEIAIDNVVSEIEALCK"
BASE COUNT         1418 a          859 c          863 g         1484 t
ORIGIN      
        1 ttgccttgcc agcatcgtct ttggcttcaa tctttagaga aacggtgctg tccttcttcg
       61 ccttttccga ggcaccagca tcctcatatg taaaataatg ttctctaaac tggtctaact
      121 gagcatcaat atctttttca gtaggctccg ccgaaggcaa gctaatcttg actttgtcat
      181 aactgcttag tgtaaactta ggctctacca ggattttata agaaaaactg taggcttttc
      241 cttcctcaac aagctttgat tcttccttgc caaagtcagg ttgtccaaca gggaaaatat
      301 tagcctcatc aatagataac ggataattct tgttgatagt atcatccgta acctgggcca
      361 acacaccttc cttagtaaac atattgtcaa ctactggacg aggagccttt ccagcacgaa
      421 agccaggaaa cttgtatttt tttgcaagct ccgcgtattt gtcagaaatt gctttgttta
      481 cctcgtcttt tgtgaagtca actacaactt taacgttatt gtcttttaaa ttttctactt
      541 ttactttcat ctaattccca tcctatttca tatccatcac aggcgccaaa atccaaatta
      601 ggcgcaaccg catattatcg cacaaaaacc caggaaatca cccactatta tgcttatcaa
      661 taatgcaata aatctttagt gatacaataa tacttgtgga aaagaacaca atacataaaa
      721 ttaacataac atcctactca agtgaaggcg aaggaattgg gcgaattgat ggaatggctg
      781 tgtttgtaaa gggtggaatg attggcgaaa ctcttgaagt taaaattaca aaagtagcta
      841 aaaattgtgc ttgagctcgc ctaaataaag ttctcaaacc atcgcctgcc agagtaaatc
      901 cagattgcga gtttgcttcg gcatgcggag gatgctctct tcgacatatg aattacgaag
      961 aagagctgca actcaagctt cggcgtgtta acgatgctct tcaaagaatt ggcaagctag
     1021 acttcaaaat taataagatt cacggagctg acaacatact tcgctacaga aataaggcaa
     1081 cttttccagt tggttatgac aaaaatgcaa attttaaaat tggaattttt caacaacgaa
     1141 gccacaatat catcgacatt aatgaatgtt ttattcaaag cgaagaagcc gaagccattg
     1201 ttggtatcat tcgtaaatga atgcttgaac ataaaattcc agcttacaat gaggaacagc
     1261 aaactggtct aattagacat ttgtttgttc gtactaataa taaaaaagaa gttttggtat
     1321 gtttggtaac aacaaaaaaa gaaattcctc atttggatac tttaataaca ttatttaaaa
     1381 attcaactat tgatattgtt ggactagttc aaaacattca agatgaaaac accaatgtga
     1441 tttttggcaa acaatttaat acgctctgaa agaaaaatta cattaaagat aaaattttag
     1501 atttagattt taagctaagt gttgcttcgt ttttccaagt aaatagagca caagctgaaa
     1561 aactctatac aattgcttta gattatgcaa acattaaagc aaatgaaact gttgtagatt
     1621 tatattgcgg aacaggaact attaccctgc ttatgtcaaa aaaagctaaa cgggcttttg
     1681 gcatagaaat tattcctgaa gcaattttag atgctaagca aaatgccata attaattcaa
     1741 ttgataatgc tgattttata tgtgctgatg caaaaacagc cgccgaagat ttcgcaaata
     1801 ataaatttaa tcctgacgta atctgtgttg acccgccaag aaaaggcctt gacataagta
     1861 caatatcttc aataattaaa atgtcaccaa aaaggattgt gtatataagt tgtgatcctg
     1921 caaccttggc cagagacttg caaatctttt caaaatcaaa ttacaagatt gtgaaagccg
     1981 aagcgctcga catgtttcca aggacgcctc acatagaatc agttgtctta cttacaaagt
     2041 aagttaaaag tatcgaagtt ttgttataat ctcttggcgt tgtattaatc gaaggagaaa
     2101 ttatgttttt aggtcttacc gtagatcaat gagcagcaat tgctatcttt gttgttgcga
     2161 tggtcattat catgagcgaa aaaatccatc gttcggtagt tgcaattgtt ggtgcaatga
     2221 ttgttattat tgcaggtctg gttgtttttg cagaccccga aggcgccgaa actgttgcgg
     2281 gtgcagttcc gattgatgaa attatcgact taaacacgct tggcgttttg tttggaatga
     2341 tgctatttgt tgccgttgtt aagcaatctg gtattttcga ataccttgca attaaatgcg
     2401 cgagaattgc ccagggtaaa gcttgaagag tcatgatgct tttcataatt cttacagctg
     2461 tgctttctgc atttttagac aatgtaacaa ctgttttgct catcggacct atgactctta
     2521 caatttgtaa attgatgaag caaaatcctg tgccattttt cctaacacaa attttagcat
     2581 ctaacattgg tggtactgca acattgattg gtgatccacc taatatcatg attggctctg
     2641 ctgctaacct atctttcgct gactttatta tgattgatgg accttgcgtt gttgtcattt
     2701 tggttattgt tattgcgatg ttctacttta tgtatggtcg caagatggaa gttcatgcag
     2761 acgatagaga aaaaatccgc actatggatg caagcaaaga aatcaaaagc aaatacctaa
     2821 tgactctaag cgttatcatg atttgcttgg ttgttgtatt ctttatgatt catgacacac
     2881 taggtcttaa atcaagcatt attgcaatga gtgctgctgg cgttatgctt cttctttctc
     2941 gttgcagcgt tgaaaaagct cttttggatg ttgagtggac aacactttca ttcttcgcag
     3001 ggctttttgt tattgtcggc gcattaaagc aaactggcgt tattgcgatg ctagcccagg
     3061 cattagttga tgcaaccggt ggcgatgtct tcgttcttat gttagttctt ttagttggtt
     3121 cagctgtaat ttctgctttc ttagacaaca ttccattctg tgcaactatg cttccaatta
     3181 ttgctgctat gcaaggaact atcgatgtta cacctctttg gtgggcagtt tcacttggtg
     3241 cttgtcttgg tggaaacgga acacttattg gtgcaagtgc aaatgttgtt ctttctgata
     3301 ttgctaagaa gaatggttac ccaattacct ttatgcaatt tacgaaggtt gggttcccaa
     3361 tcatgttagt tactgttgcg attgcagcag gctacatgac attgcgatac ttcactatcg
     3421 gcttttaatc aaagggctcc ttcgggagcc ctttttatat tatggataaa gtgatagtaa
     3481 atacattaaa tgaattagat gaattctgta aaggattctg ccaatcatta aaacctggcg
     3541 atgttatttg cttaactgga gatttaggtg ctggcaaaac tgcatttgtt cagtcagtcg
     3601 caaaagcttt taatatcact gatcaagttc agagtccaac ttttaatatc ttgttgcagt
     3661 ataaaaacga cgaagttgct ataaatcact tcgatttata tcgcttagaa aaagcacaag
     3721 aacttgagga tatcggcttc tacgaagccg tggaaagcaa agcttttagc ttcgttgaat
     3781 gggcagaaaa attcaaagat caaatgcctg aagattcaat ttgaattaca ataaaaaaag
     3841 ttgatgaaac tactagagaa tttcaagtag ttcagaagga tgagaaataa tatagtttgg
     3901 gttttcggat gcgagggttt cgcgatctga aacaccccaa agcactccac aagttttaca
     3961 tccggcgcca tttccagctt gtagatcata aatactatcg ccaatataga ttgtttcttc
     4021 gggggtaaaa ccaatttctt cacatgcatg agttagtgca cctgcatttg gtttatgaac
     4081 atctcaatca tctggacctt gaatatatgg aaaatattta tcaatgccca aacgtgttaa
     4141 gccctcaagg cacacatcat gcaacttcga agttacaata cccatataat aaccgcggtt
     4201 tttaagttct tctagaactt cgggaaggcc ttcgaaattt ttggacatgt caaacaagat
     4261 tttattgtta tgagcgcgat aagttctaat catcaactga attgtgtctt cgtcatcggt
     4321 aaattcttta aattggtcat aggatggaag acctattaag gactggaact tagcaatgtc
     4381 atcttcgctg caatgaccta aaacttcgtt caacatatat ctgactgacg tcaagattgg
     4441 ctctaaggtg tcaaccaccg tgccgtcgtt atcaaaaagt attgctctaa tctttgacat
     4501 tacttacaaa gcgcttcaat ttcactaaca acattatcaa tagcaatctc gctacgctcg
     4561 ttgctcttac gaatctttag ctcaacgttt ccattttcaa gactcttctt gcctaaaaca
     4621 attt
//
LOCUS       DBNCDC010000088         4567 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_12161_length_4567_cov_7.427970, whole genome shotgun sequence.
ACCESSION   DBNCDC010000088 DBNCDC010000000
VERSION     DBNCDC010000088.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12161_length_4567_cov_7.427970"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /gene="proS"
                     /locus_tag="AC04VQ_06270"
     CDS             complement(<1..1202)
                     /gene="proS"
                     /locus_tag="AC04VQ_06270"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIO9553720.1"
                     /translation="MNNVIYMKDLYAPTLKEVPSEAEIPSHKLMLKAGYIRKTASGVY
                     TFLPLAKRVLEKIEDIIRDEMDATGAQEILMPALQPAELWHESGRWNDYGPELMRLKD
                     RHEHDFCLGPTHEEVITSLVRNELKSYKQLPTTLYQIQVKFRDEIRPRFGLLRGREFM
                     MKDAYSFHSSQEDLDKMYARMMKAYENICERCGLEYRAVEADSGQIGGSVTCEYMALA
                     ENGESDIAYCDCGFATDTEVLLNGEEPAEDQEYTCPRCGKKCETARGIEVSQVFQLGT
                     KYSEAMNVNYVGEDGKEHPFIMGCYGVGITRLMAAAIEQWNDENGMIWHPNIAPAEVC
                     LIQLTDNVELQDKLVKELTDKGIQVCIDDRDERAGFKFADADLIGWPFQIVLGKKSLE
                     NGNVELKIR"
     gene            complement(1233..1547)
                     /locus_tag="AC04VQ_06275"
     CDS             complement(1233..1547)
                     /locus_tag="AC04VQ_06275"
                     /inference="COORDINATES: protein motif:HMM:NF053397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553721.1"
                     /translation="MLSALDLRRQYPEIISCPTCGRTKVDLIKIAREVEEKISCIKAP
                     IKVAVMGCVVNGPGEAEGADIGVACELGGGAIFKDGKIIKKVKEPEIVPTLMEEISKI
                     ID"
     gene            complement(1551..2291)
                     /locus_tag="AC04VQ_06280"
     CDS             complement(1551..2291)
                     /locus_tag="AC04VQ_06280"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF016438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIO9553722.1"
                     /translation="MEKRQTYEVNVGGIKIGGANPVVVQSMCCAPAEDFDKNLKQINE
                     LAAAGCELVRVSVPSDNALKAFAELCKKSPLPLVADIHFRPDLAIKSIEAGASKIRIN
                     PGNLGGLEKTDEIISSAKQNKVPIRIGVNAGSLDEELKARTDLTLPEKLVASASQFVE
                     YFNNHDFVDIVVSIKAHDVNTCVEANRLFAKQMSKVPLHLGITEAGTKDQGIIKSASG
                     LGILLSEGIGETIRISLTDNPVEEVKAA"
     gene            2364..2957
                     /locus_tag="AC04VQ_06285"
     CDS             2364..2957
                     /locus_tag="AC04VQ_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TroA family protein"
                     /protein_id="HIO9553723.1"
                     /translation="MKLQAKNIFIGIFLISVIAILTLILSSCNYKEIRDANETITDFN
                     GRNVQYPSNVERIATVGSAARSVVYAGAQNKLVAITEMDRPTELRPYTLVDPELFSSL
                     PTTSDGNHLNSTNIDKEKLLELNPDVILSSRSKSECERLQNDLKIPVIGVGFQDEILL
                     VDFWQSLKIVGEVCGTEVTANRRIGYIKTISDEAQKC"
     gene            3150..3452
                     /locus_tag="AC04VQ_06290"
     CDS             3150..3452
                     /locus_tag="AC04VQ_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553724.1"
                     /translation="MDYENKNKVLEQINSNPDVFSNLKAFKNNNVYFVPPFNSNGTNV
                     EYGLCELLITAKTLYPSIYGSLNLDEAYADIFENLLGKNIYPELLNRGIVIGKASF"
     gene            3520..4461
                     /locus_tag="AC04VQ_06295"
                     /pseudo
     CDS             3520..4461
                     /locus_tag="AC04VQ_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013219.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
BASE COUNT         1385 a          936 c          812 g         1434 t
ORIGIN      
        1 cgaatcttta gctcaacgtt tccattttca agactcttct tgcctaaaac aatttggaat
       61 ggccaaccaa tcaaatccgc atcggcaaat ttaaagccag cacgttcatc cctatcatca
      121 atgcaaactt gaattccttt gtcggttaat tccttgacaa gtttatcttg aagttcaaca
      181 ttatctgtaa gttggattaa gcaaacttcg gcaggggcaa tgtttggatg ccaaatcatt
      241 ccgttttcat cgttccactg ttcaattgca gcagccatca aacgagtaat tccaacacca
      301 taacaaccca taataaacgg atgctctttt ccatcctcac caacatagtt aacattcata
      361 gcttccgaat atttagtgcc aagttggaaa acttgagaaa cctcaatacc tcttgcagtt
      421 tcacatttct taccacacct agggcaagtg tattcctgat cttcggcagg ctcttcgccg
      481 ttaagaagga cttcggtgtc tgttgcaaaa ccacaatcac aatatgcgat gtcggattcg
      541 ccattttcgg ccaaagccat gtattcacaa gtaacacttc cgccaatttg accactatca
      601 gcctcaacgg cacgatattc tagcccgcat cgctcacaaa tgttttcgta agctttcatc
      661 atgcgggcat acatcttgtc caaatcttcc tgactactgt gaaaactgta agcgtccttc
      721 atcataaact cacgaccgcg caaaaggccg aagcgaggac gaatctcgtc acggaattta
      781 acttgaattt gatacaaggt tgttggaagc tgtttgtaag acttcaattc atttcgaact
      841 aacgaagtaa taacttcttc gtgtgtaggc ccaagacaaa aatcatgctc atgcctatct
      901 tttaggcgca taagctctgg accataatca ttccagcgac cactttcatg ccaaagctca
      961 gcaggttgca aagcaggcat caaaatttct tgagcgccag ttgcatccat ttcgtcacga
     1021 atgatatctt caatcttttc taaaacgcgt tttgccaaag gcaagaatgt ataaacacca
     1081 ctagcagttt tgcgaatata tccagcttta agcatcagct tatgactggg aatttcagct
     1141 tcgcttggaa cttccttcaa agttggagca tacaaatctt tcatataaat tacgttattc
     1201 atttttgcct tccttatcaa acgtatattt tgttagtcga ttattttact aatttcttcc
     1261 attaatgttg gaactatttc tggctcttta actttcttta tgattttgcc atctttaaaa
     1321 atagctccac ctccaagttc acaagcaact ccaatgtcgg caccttcggc ttcacctggg
     1381 ccattaacaa cacatcccat aactgcaact ttaattggag ccttaataca agaaattttt
     1441 tcttcaactt ctctagcaat tttgataagg tcaacttttg ttcgaccaca agttggacaa
     1501 gaaataattt cgggatattg tcgacgaaga tcaagagcgc tcagcaaagt tcaagccgct
     1561 tttacttctt cgacaggatt atctgttagc gaaattcgaa ttgtctcacc tattccttcg
     1621 ctaagcaaaa ttcccaaacc agatgcagat tttattatgc cttgatcttt cgttccggct
     1681 tcggtgattc caagatgaag aggaactttt gacatttgct tagcgaataa tctattggcc
     1741 tcaacgcaag tgttaacatc atgagcctta attgacacaa caatatcgac aaagtcatga
     1801 ttgttaaaat attcaacaaa ctgcgaagcc gaagccacaa gtttttccgg taatgtaaga
     1861 tcagttcttg cttttagttc ttcgtcaaga gaaccagcat taactccaat acgaattgga
     1921 actttgttct gcttcgctga acttattatc tcgtcggtct tttcaagacc accaagattg
     1981 ccaggattga ttctaatttt cgaagcaccc gcctctattg atttaattgc taaatcagga
     2041 cgaaaatgaa tatcagcaac cagcggcaaa ggagattttt tacaaagttc agcaaatgcc
     2101 ttaagggcat tatcagatgg aacactaact cgaacaagct cgcaacctgc ggcagcaagc
     2161 tcattaatct gctttaagtt tttatcaaaa tcttcagctg gcgcgcagca catgctttga
     2221 actacaacag gattcgcgcc accaatcttg attccgccaa cattaacttc gtatgtttgc
     2281 cttttttcca cgaaataaaa ttatacacca aaataaaaac tacacattat ataagcagta
     2341 gtaaatgtga taaaatccat tttgtgaaat tacaagcaaa aaatatattt attggaattt
     2401 ttcttatttc agttattgca attttgacct taattctaag tagttgcaat tacaaagaaa
     2461 ttcgagacgc aaacgaaaca atcacagatt ttaacggaag aaatgttcaa tatccaagca
     2521 atgttgaacg tattgcaaca gttggttctg ctgcaagatc cgttgtttat gcaggggctc
     2581 aaaacaaact tgttgccata accgaaatgg acaggccgac tgaacttcgt ccctatactc
     2641 ttgtggaccc ggaattattt tcgagtcttc caacaacaag cgatgggaat cacttaaata
     2701 gcacaaacat tgataaagaa aaacttttag agctaaaccc ggatgtgatt ttgtcttcgc
     2761 gaagcaaaag tgaatgtgaa aggcttcaaa atgatttaaa gattccagtt attggagttg
     2821 gcttccaaga cgaaattcta ttggttgatt tttggcaatc tctaaaaatt gttggcgaag
     2881 tttgcggaac tgaagttacg gccaacagaa ggattggata tattaaaacc atttctgacg
     2941 aagcacaaaa atgttgaggt gttgcttcgc caagcatata tagaggcgca atcaactaca
     3001 aaggttctaa agatttaact ggaacctatt cacactattg cgtttatgat gcaattagaa
     3061 aatcaggcgt ggcggacaga gatgatatcg agggagctta tgacacaacg ctcgagcaaa
     3121 tcttgacttg aaatcccgac tacattttta tggactacga aaacaaaaac aaagtccttg
     3181 agcaaataaa ttctaacccg gatgtttttt caaatcttaa ggcatttaaa aacaataatg
     3241 tatatttcgt cccaccattt aacagcaatg gaaccaacgt agaatatgga ctttgcgaac
     3301 ttcttataac tgcgaaaact ttatatcctt ccatatatgg atctctaaat ttagacgaag
     3361 cttatgctga tatctttgaa aatctacttg gcaaaaatat ttatcctgaa cttttaaata
     3421 gaggaatagt aattggcaaa gcctcattct aaaaatattg atcttaatat tgatataact
     3481 catgtctaca ataaactttc gcgtaggaaa tgatgagcct tgcttgctgt tattactctg
     3541 acaattattc ttttaacgat tgatttatgc attggctctt caacaatgag cctgtttgat
     3601 cttattaaaa cggcaattaa tcaaccgtca agtgaagcat atattatttt atacacatta
     3661 agatttccgc atgttctatg ttcgctaatt gcaggttgca tacttggaat ttgtggatgc
     3721 attttacaaa gttcacttgg aaaccctttg gcggcgcctt cgactattgg agtttctcaa
     3781 ggtgcggctt gtggtgcttg cattgcaata atttgcggag gaagttttct tagtggatct
     3841 atttgagttg ttgtgtttgc ttttattttc gcaatgattc caacgctaat aatattttta
     3901 atttcaaaac tacgaagctt tagcgcaacg agcattatcc ttgccggaat agcaatggca
     3961 atattttttg gaggcgttgt tgctttggtg gaatacttcg ctgatagttc aaaagttgcc
     4021 gaagttgtgt tttggacctt tggaagtgtt tacaatacgc aatattctga ccttatcttt
     4081 atggcaataa tgctacttat agtatgcatt tattcgatgc taaacttcat taacttcaac
     4141 gcattagaat gtggagacat gaccgctcac agcgtgggaa taaacgtccg aagttcaaga
     4201 cttcgtcact taattattgc ggctttgggt gcggcatgcg taactgcatt ttgcggaacc
     4261 ataaatttca ttggcttaat tgctccacac attatgcgaa gattcgttgg atcaaattat
     4321 aagtacttaa ttcctacaag cgctttatgc ggtgcttgca ttttaatttt aagcgatatt
     4381 ctttctgcag gattattgcc aggagttatt ttgccaattg gcgctatcac aagttttatt
     4441 ggtgcgccaa tgtttatttg aatattaatg aaacgagcga agtaatggcc gcaactcaaa
     4501 acatattgtc agttaaaaac ttgagttttt cttacaacaa gagcgagcct gtgcttcgta
     4561 atgttaa
//
LOCUS       DBNCDC010000089         4520 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_12381_length_4520_cov_9.385442, whole genome shotgun sequence.
ACCESSION   DBNCDC010000089 DBNCDC010000000
VERSION     DBNCDC010000089.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12381_length_4520_cov_9.385442"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            48..800
                     /locus_tag="AC04VQ_06300"
     CDS             48..800
                     /locus_tag="AC04VQ_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006362461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HIO9553725.1"
                     /translation="MLIEWVNTLVGDWGLAIIIVTIIFRGIMLPIMHKQTKSSYMMKK
                     MNPEMNRIKELYADDQKRQSEELQKVYAEAKFNPISGCIPMFIQMPIFIALFQALREV
                     PDRIGDVSNYCFFNLVPNLVCSPQDMWNQMDKIGILPIIPYIILIVIFAGATFLPMIL
                     QQQGGDPKQRKQTLIMSGVMSVVMLFVGWSSPAGVLLFWGTSSILALIQQRVSMKILE
                     NKDKQLEEEKEYQPVKVDVVRKEKTKRPRKKK"
     gene            816..1385
                     /locus_tag="AC04VQ_06305"
     CDS             816..1385
                     /locus_tag="AC04VQ_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Jag family protein"
                     /protein_id="HIO9553726.1"
                     /translation="MVDEEKLEQEKIEESQKEMAEEQEAIAKREIPQLSDEELDNVAD
                     TAIDYIQEIVSNFNLGEITINEYEGADKELILDINGNDLAILIGKHGATLDSIQHIVG
                     VLSYKKLGFYYPVVIDVEGYKSRQRIKLENLAKNLANRVARNKKPYAMHPMNPFKRRI
                     VHTYLSSDDRVETHSEGEGRNRHLVITPK"
     gene            1392..2057
                     /gene="rsmG"
                     /locus_tag="AC04VQ_06310"
     CDS             1392..2057
                     /gene="rsmG"
                     /locus_tag="AC04VQ_06310"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015539279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIO9553727.1"
                     /translation="MEITKEQNALLEKHLDLVLFKNQQLNLTAIKDKDEAKVLHLEDS
                     LAGLEELNSVEGEFVDMGSGAGFPGIPLSIVSGKKATLVETVGKKADCLESFINELNL
                     EAQVSVSNKRIEELAVERPNSYGAATARALSSLSSLLELASPLLVINGLLICYKGNNV
                     EEELNAALNITEKLGFEFVSNRKYMLSDSTEHRIVVFKKVKEPEVKLPRKLGFAQKKP
                     FTN"
     gene            2155..3183
                     /locus_tag="AC04VQ_06315"
     CDS             2155..3183
                     /locus_tag="AC04VQ_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIO9553728.1"
                     /translation="MGFFDSFKKKNQPETSNYQQEDNNIEEQDQYDAAVTKRKVVEDE
                     PKKEVVEFEAEKPVEQEKPQETEPVEKPKPVEPSLPKEFAKPSGGPSKILTIINQKGG
                     VGKSTTAINLAAALGEDKKHVLLVDLDPQGNSTSGLGIDKRSIKRCIYDVLLNDAGIE
                     DVVVKDAYKNVDVVPATINLAGAEVELVTEIARENRLKEALGKVKANYDYIFVDCPPS
                     LGLLTVNALVAADKLFIPIQTEFYALEGVTKLLDSMKRVKTRLNPKLDIFGVLLTMYD
                     GRTTLAKQVSDEVRKYFKDLVFDTKIPRTVKISEAPSFGQPITQYEPSCKGAIAYKNL
                     AKEVMNRG"
     gene            3176..4186
                     /locus_tag="AC04VQ_06320"
     CDS             3176..4186
                     /locus_tag="AC04VQ_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009608935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIO9553729.1"
                     /translation="MAKKGLGRGLNSLLGGDSYEEDIRYNIDSVDQVDDYSDEIEGFD
                     GEVKIREAEPKKVAKKPAKKAETKKAKPAGQGVLEVSISQVVANPDQPRKNFSQQELD
                     ELADSIKRNGLLQPILVSAKDDKYEVIAGERRLRACKIAGLKKVPVIVKQTSKNKKLE
                     LALIENIQREDLNPMEEAYSYKKILDTQGISHSELASIVSKARSTITNSLRLLELPED
                     AQKLLYENKITAGHARAILSVSTQMGKEKLSKKIVDEHLSVREAESLANLYNGQNTKA
                     LTTVRPAAPKSYKKAAKLLRDHLGNNVRVKKAKGKNKIEIEFSDERDLERILGLIVND
                     KK"
BASE COUNT         1549 a          741 c          934 g         1296 t
ORIGIN      
        1 agcaggtagt tatgaaccca tgagatttat ttaaagatgg aattttcatg ttgatagagt
       61 gggtcaatac cctcgttggt gactggggcc tcgctattat tattgttacc attatcttta
      121 gaggcatcat gctgcctatc atgcataagc aaaccaaatc atcttatatg atgaaaaaaa
      181 tgaacccaga gatgaataga attaaagagc tttatgcaga tgaccaaaaa agacaaagcg
      241 aagagcttca aaaagtttac gccgaagcga agttcaaccc aatttctggt tgtattccta
      301 tgtttattca aatgcctatt tttattgcgc tcttccaggc acttcgtgaa gttccagacc
      361 gcattggtga tgtttcaaat tattgttttt tcaatcttgt tcctaatctt gtttgttcgc
      421 ctcaagatat gtggaaccag atggacaaga ttggaatttt gccaattatt ccatacatca
      481 ttttgattgt aatttttgct ggtgcaacat ttttaccaat gattcttcaa caacaaggtg
      541 gcgatcctaa acaaagaaag caaaccttga ttatgtctgg tgttatgtcg gtggttatgt
      601 tgtttgttgg ttggagctct ccagcaggcg ttttgctgtt ctggggaact tcgtcaatcc
      661 tagctttgat tcagcaaaga gtaagtatga aaatactcga aaataaagat aaacaactcg
      721 aggaagaaaa agaatatcag ccagttaagg ttgatgttgt tcgcaaggaa aaaaccaagc
      781 gtcctcgaaa gaaaaaataa tatgaggtga ttgatatggt tgatgaggaa aaattagagc
      841 aagaaaagat cgaagaatct caaaaagaaa tggccgaaga acaggaagcc attgcgaaac
      901 gtgagattcc gcagctaagc gatgaggaac ttgataatgt tgctgatact gctatcgatt
      961 atattcaaga gattgttagc aatttcaacc ttggtgagat aacaattaat gaatacgaag
     1021 gtgctgataa agagctaatt ttggatatta atggcaatga tctggctatt ttaattggca
     1081 aacatggtgc aactcttgat tcaatccaac acattgttgg cgttttgtct tataaaaaac
     1141 ttgggttcta ctaccccgtt gttattgacg tagaaggtta taagagcagg cagcgcatta
     1201 aattagagaa tcttgcgaag aatttagcta atcgtgttgc gcgcaataaa aagccatatg
     1261 caatgcatcc aatgaatcca ttcaaaagac gcattgttca cacatatttg agctcggatg
     1321 atcgtgtaga aactcattcc gaaggcgaag gccgcaacag acaccttgta attacaccta
     1381 aataagccaa tatggaaatc acgaaggaac agaatgcatt gctcgaaaag catctggacc
     1441 ttgtgctttt taaaaatcag caactaaatc taactgcaat taaagataaa gacgaagcaa
     1501 aagtcttgca tcttgaagac tctcttgctg ggcttgaaga attaaactct gttgaaggag
     1561 aatttgttga catggggtcg ggggcaggat ttcccgggat cccattaagt attgttagcg
     1621 gaaagaaagc tactttagtt gaaactgttg gcaaaaaagc tgattgtcta gaatccttta
     1681 ttaatgagct aaatttagaa gcacaggttt ctgtcagtaa taaaagaatt gaagaacttg
     1741 ctgttgagag gccaaattct tacggcgctg caactgcaag agcattatct tcgttgtcat
     1801 cattacttga gcttgcttct ccccttttag ttattaacgg gcttttaatt tgctataaag
     1861 gaaataatgt cgaagaagag ttaaatgctg ctttaaacat tactgagaag ttaggctttg
     1921 agtttgtttc aaatagaaaa tatatgctaa gcgatagtac cgagcacagg attgttgttt
     1981 ttaagaaagt taaagaacct gaagtcaaac tacctagaaa gcttggattt gctcagaaaa
     2041 agccttttac taactaatat gtttcacgtg aaacatcttt aaaataatag ttgaaaaata
     2101 actgttttgt attattatac caagtgtaaa cgtagcttaa acgaaggagg atatatgggt
     2161 ttctttgact cgttcaaaaa gaagaatcaa cccgaaacgt ctaattacca gcaagaagac
     2221 aataatattg aagaacaaga tcaatacgat gctgctgtta caaaacgtaa agtcgttgaa
     2281 gatgaaccaa agaaagaagt agtagagttt gaagctgaaa agcctgttga gcaagaaaag
     2341 cctcaagaaa cagagcctgt tgaaaagcca aagccagttg aaccttctct tccaaaagaa
     2401 tttgctaaac catctggtgg accttcgaag atactaacta ttattaatca gaagggtggc
     2461 gttggaaaat ctactaccgc aattaatctt gctgcagctt taggtgagga taaaaagcac
     2521 gttttgcttg tagatttaga tccgcaaggg aattcaacaa gcgggcttgg tatcgataaa
     2581 agaagcataa agcgttgcat ttatgatgtt ttacttaacg atgctggtat tgaggatgtt
     2641 gtagttaagg atgcatataa gaatgttgac gtagttcctg ctacaattaa tcttgctgga
     2701 gctgaagttg agcttgtaac tgagatagca agagaaaaca ggcttaaaga agcattggga
     2761 aaggtgaaag caaattacga ctatatattt gttgattgtc cgccttcgtt aggtttatta
     2821 actgtaaatg ctcttgttgc tgcagataag ctatttattc caatacagac agagttttat
     2881 gcacttgaag gagtgacaaa acttcttgat tcaatgaaac gtgtcaaaac tcgcctaaat
     2941 ccgaagcttg atatcttcgg tgtcctgcta acaatgtacg atgggcgcac tactttggct
     3001 aaacaagtat cagatgaggt gcgtaaatac tttaaagacc ttgtttttga tacaaaaata
     3061 ccaagaacag ttaagatttc cgaggctcca agcttcggac aaccaataac gcaatatgaa
     3121 ccatcatgta aaggtgctat tgcatataaa aatctagcaa aagaggtgat gaatcgtggc
     3181 taaaaaagga cttggaagag ggctaaattc cctgttggga ggagactcct acgaagaaga
     3241 catcaggtat aacatcgatt ctgtagatca ggttgatgat tactctgatg aaattgaagg
     3301 ttttgatggc gaagtaaaaa ttcgtgaagc agaacctaag aaagttgcta aaaaaccagc
     3361 taaaaaagca gaaactaaaa aagcaaagcc agctggtcaa ggcgttttgg aagtatcaat
     3421 ttctcaagtt gttgctaatc ctgatcaacc aagaaagaac ttcagccagc aagaacttga
     3481 tgaacttgct gattctatca aaagaaatgg cttattacaa ccaattttag ttagtgcaaa
     3541 agatgataaa tatgaggtca tagctggtga aaggcgtctt cgtgcttgca agattgctgg
     3601 tcttaagaag gttccagtta ttgttaaaca aacaagcaaa aataagaagc ttgagcttgc
     3661 tttgatagaa aacatccagc gtgaggacct aaaccctatg gaagaagctt atagttataa
     3721 gaaaatcctt gatacgcagg gcatttctca ttcagaactt gcttcgatag tgtcaaaggc
     3781 caggtcaaca ataacaaatt cacttcgtct attagaactt cctgaagacg ctcaaaagct
     3841 tttatatgaa aataagatta cagctggaca tgcaagagca attctttcgg tttctacaca
     3901 gatgggtaaa gaaaaacttt caaaaaagat tgttgatgag catttatctg ttcgtgaagc
     3961 agaaagtctt gctaatttat acaatggcca aaatacaaaa gcattaacca cggttcgtcc
     4021 tgctgctcct aaatcatata aaaaggcagc taaactatta agagatcatc ttggcaataa
     4081 cgttagagtt aaaaaagcaa aaggcaagaa caaaattgaa attgagttta gcgacgagag
     4141 ggatttagaa agaatcttag gcctaatagt taacgataaa aaataaaacg gccttaaata
     4201 gcatttaaga ggctcgaaat cgggcctctt tttttatgtt tcacgtgaaa catctaattg
     4261 agattattta gaagctgtcc gcaagcggca tctatatcag aaccgcgaga agcacgcagt
     4321 gtggcttcga tacctttatt gtttagatct ttaattcatt tgtcaacaac agtattagag
     4381 gaagccttaa actttgaatc tttgacattg ttgaatttaa ttaggtttat atgacaaagc
     4441 ataccttcgc agaaattaat taaagcattt agatcttctt gtgtatcgtt tacattgtta
     4501 atcaaaagat attcaaacgt
//
LOCUS       DBNCDC010000090         4432 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_12855_length_4432_cov_5.820653, whole genome shotgun sequence.
ACCESSION   DBNCDC010000090 DBNCDC010000000
VERSION     DBNCDC010000090.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12855_length_4432_cov_5.820653"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            212..361
                     /locus_tag="AC04VQ_06325"
     CDS             212..361
                     /locus_tag="AC04VQ_06325"
                     /inference="COORDINATES: protein motif:HMM:NF015674.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HIO9553730.1"
                     /translation="MALICGGNFCAYPFSALSAISVVTGVFLIFAGASNIITIFFDRK
                     KIKWW"
     gene            complement(364..567)
                     /locus_tag="AC04VQ_06330"
     CDS             complement(364..567)
                     /locus_tag="AC04VQ_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553731.1"
                     /translation="MPVNIPSKRSGFQPSPFSFLMLIAERISITASEANPNPVKNHNN
                     FKENEWRATKASPTTIIKTLKII"
     gene            506..697
                     /locus_tag="AC04VQ_06335"
     CDS             506..697
                     /locus_tag="AC04VQ_06335"
                     /inference="COORDINATES: protein motif:HMM:NF015674.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553732.1"
                     /translation="MKKEKGEGWKPLLLLGILTGIAAIVILTDPIFGLRFVSLFVTIG
                     VICFGIKLILFAFQTKPKK"
     gene            complement(698..1549)
                     /gene="prmC"
                     /locus_tag="AC04VQ_06340"
     CDS             complement(698..1549)
                     /gene="prmC"
                     /locus_tag="AC04VQ_06340"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIO9553733.1"
                     /translation="MSDISDISKALNFGKDYLSRSNVNDAELSAEILLAEALGLNRQS
                     LYSHFEDKLSDVQQELYKEYISRRANHEPLQYLLGYAYFRGLKITVGPGVLIPRPETE
                     QLVEVVIKNLKNNCNKILDLCTGSGCIACSFANEFSDVKIYGTDISGEALTYAKMNIK
                     KHHFEEKITLVQCNMADEIYDNNFDLIISNPPYVPTDVYKNLDKEVIDYEPKLALEAG
                     SDGASLLDEIIATAKDKLKKDGIVALEFHEDKLDLAKSKFETAGFNDCKINKDLAGKD
                     RILIAKK"
     gene            complement(1549..2625)
                     /gene="prfA"
                     /locus_tag="AC04VQ_06345"
                     /pseudo
     CDS             complement(1549..2625)
                     /gene="prfA"
                     /locus_tag="AC04VQ_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
     gene            complement(2625..3626)
                     /gene="thrS"
                     /locus_tag="AC04VQ_06350"
                     /pseudo
     CDS             complement(2625..3626)
                     /gene="thrS"
                     /locus_tag="AC04VQ_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
     gene            complement(3639..4379)
                     /locus_tag="AC04VQ_06355"
     CDS             complement(3639..4379)
                     /locus_tag="AC04VQ_06355"
                     /inference="COORDINATES: protein motif:HMM:NF019585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553734.1"
                     /translation="MATKKKENNEDQLMTMRHSCAHVLAEAVQKLYPGTKIGFGPATE
                     DGFFYDFDLPKPISSDNFGKIEKEMKKIIAANNKFEREVVTKAEAKKIFKDQDLKLEH
                     IDDLPDNEEITVYKHGDFVDLCAGPHVESTKEIGAFKLTKTAGAYWKGDANNKQLTRI
                     YGTAFFTQDDLDEYFTRLEEAEKRDHRKLGKELGIYTMSEMAGVGLPMYLPNGAIIIR
                     KMQDWLRNDLIDRGYQEVITPHIYNADV"
BASE COUNT         1238 a          967 c          779 g         1448 t
ORIGIN      
        1 ttccccaacg gtttttgaga ccgccgcgtc tgccgttccg ccactcgggc aacaagacgt
       61 attataacga ttaacgcttt attcgcaata acaatttttt gtattttaga gtataatatt
      121 ggcatcaaaa gaagacagtg aggagtttgt catggcaaag aaaatcgata gaagttcgaa
      181 atattggtga ctgtatttag tttttggcat tattgcccta atttgcggag gtaatttttg
      241 cgcctaccct ttctctgcgt taagtgctat atcagtagtt actggtgtgt tcttaatctt
      301 tgccggagct tcgaacatca taacaatatt ttttgacaga aagaaaataa aatggtggtg
      361 actttatata atcttaagcg ttttaataat cgtggtagga cttgctttgg tagcacgcca
      421 ttcattttct ttgaaattat tatgattctt aacaggattt ggattcgcct ctgaagcagt
      481 tatcgaaatt ctttctgcaa ttaacattaa aaaagaaaaa ggcgaaggtt ggaagccact
      541 tcttttgctt gggatattaa caggcattgc cgcaattgtg atattaacag atccaatttt
      601 tggcttgaga tttgtttctc tgtttgtaac aattggtgta atttgttttg gaattaaact
      661 aatcttattt gcgttccaaa caaagccaaa aaaataatta ttttttagcg attagaattc
      721 tatctttacc agcaaggtct ttattaattt tgcaatcatt aaaacctgct gtttcaaact
      781 ttgactttgc aaggtcaagc ttgtcttcgt gaaattcaag agcaacaatt ccgtcttttt
      841 tgagcttgtc ttttgctgta gcaattattt cgtcaagcag cgaagcgcca tcgctacccg
      901 cttcaagtgc tagcttcggc tcatagtcaa taacttcttt gtctaagttt ttatagacat
      961 cagttggaac atatggcgga tttgaaataa tcaaatcgaa gttgttgtcg taaatttcgt
     1021 cagccatgtt gcattgaaca agtgttattt tttcttcaaa gtgatgtttt tttatattca
     1081 tttttgcata agttaatgcc tctcctgaaa tgtctgtacc gtaaattttg acgtcagaaa
     1141 actcgtttgc gaaagaacac gcaatgcatc cgctgcctgt gcatagatcc aaaatcttat
     1201 tacaattatt tttgagattc ttaatgacaa cttcaacaag ctgttcggtt tcaggacgag
     1261 gtattaaaac accaggacca acagtaattt tcaagcccct gaaatatgca tatcctaaaa
     1321 gatattgaag tggctcatgg tttgctcttc gtgaaatata ttctttatat agttcttgtt
     1381 gtacatcgct aagtttgtct tcgaagtggc tatataaact ttgccgatta agcccaagtg
     1441 cttcagccaa taaaatttcg gctgataatt cagcgtcgtt aacattgctt cgcgatagat
     1501 aatcttttcc gaaatttagt gcttttgaaa tatctgaaat atcagacatt attctacagc
     1561 ctcagcaagt ttttgtgctt tatcagctgt ttgaagtgca gttataagct ctggaagacc
     1621 tgatccaccc tggcctaaaa ggacaccgtt ataagttccg ttatatccaa ttctgtgatc
     1681 tgtaactctg tcttgaggtc cattataggt ccgaatcttt tcagccctgt caccagatcc
     1741 aatttgagca aggcgctttg ccccttcttc ggcttgttga tcggctaaca ttttctcata
     1801 caaacgagca cgaagaacag acaaggcagc agccctattt tgaatttgcg atttttgatc
     1861 ctgcgattga acaaccaaac cagttggaag atgcgtaatt cgaactgcac tatcagttgt
     1921 atttacgcac tgaccgccag gacctccagc cctgtaaaca tcaatgcgca aatcattttc
     1981 gtcaatcttg acctcaactt cgtcagcttc aggaagcacc gcaaccgttg cagttgatgt
     2041 atgaatgcgg ccctgacttt cagtcttagg aacgcgctga actcgatgaa cgccactttc
     2101 atatttcata agagaataaa catggtcgcc cttaactttg aattgaactt ccttataacc
     2161 acccatgtca gacgcgctca tatccatagg ctcaatcttt catccctgct ctgtgcaata
     2221 acgctcatac attctataga ggtcgccact aaaaattgca gcttcgtcac cacctgcagc
     2281 agcacgaatc tcgactatga tatccttttc atcagctggg tcttcaggaa ttagcattaa
     2341 cttaatttct tcttctagtt ttggaagctg aggctcaatc gaagcgatct cttcttgagc
     2401 aacatcatgc aaatcagaat cagaaagcat ctccttggct tctgccaaat tatttacaag
     2461 ctttaaatat tccgaagctt tattcacaag cggaccttgc ttcgcaaatt ccttcgcaag
     2521 cttttgatat tcagactggt tagaaacaac ctcaggatcc cccatctttt tttgaagatc
     2581 ctcataggcc tctaatattt tctctaactt ttctttctct aacattagtg tttgctcgct
     2641 tccttaatta acttaacgaa gtcatctacg ctcatcttgc caaggtcacc tttttgtcta
     2701 tcgcgcacgg caatttcgcc agattccact tctttatctc ccataacaat catgtatgga
     2761 attttttgtg cttgagcttt agcaattttt acacgaagcg gttcgctgtc ttcataaaca
     2821 tcaactctgc cgcctacttc ataaattttc ttcttaagat catgcgcagc atccagatgc
     2881 ctatcagcaa ttgggataat ggcaacttga acaggagcaa gccacaaagg cagcgcacca
     2941 gcataatgct caattaaaat acctaagaaa cgctctatag atccgaagat tgcgcggtgc
     3001 aacatccatg gacgctccat cgtgttgtcc ttagttttat attccaagtc aaaacgttct
     3061 ggcaaattaa aatcaacttg aacagtcgag cattgccacg tgcgaccaat cgcatcctta
     3121 actttgatgt caatttttgg accatagaaa gcgccatcgc cttcgttaat ttcatattta
     3181 agattgtgag tttcgcaagc ctttttcaaa acatttgtag cgtgctccca catatcatca
     3241 gtaccgatgc tcttttcagg acgagtcgaa atctcagctt cgtaatcaaa atcaaattcg
     3301 ctcataatat ggtcaacaag tttcaaaata tcaacaacct catcgaccaa ttgttcacgc
     3361 gtgcaaaata catgggcatc atcttgagta aatccacgag cgcgaagcaa accatgaaca
     3421 acaccgctca tttcatgcct atatacagtt ccaaattcaa agaaacgaat tgggagctct
     3481 cggtagcttc gttgctctgc cttataaagc ataacatgac ctgggcaatt cataggcttt
     3541 acgccatatt cagaaagttt tggctcgtct tcggtgcctt cgttgatatt gaaaaaatac
     3601 atgttttctt tgtaaaagtc ataatgaccc gaagtctttc aaacatcagc attataaata
     3661 tgtggagtta taacttcctg gtagcctcga tcaatcaaat cattgcgaag ccaatcttgc
     3721 atcttgcgaa taattatcgc accgtttggc aaatacattg gaaggccaac acctgccatt
     3781 tcgctcatgg tgtatatgcc tagttccttc cccaactttc tgtgatcgcg tttttcagcc
     3841 tcttctaacc tggtgaaata ttcgtcaaga tcatcttgcg tgaaaaatgc tgttccataa
     3901 atacgagtta gctgtttatt gttagcatca cctttccaat atgcaccagc agtttttgta
     3961 agcttaaacg caccgatttc ttttgtgctt tcaacatgag gtccggcaca gaggtccaca
     4021 aaatcgccgt gcttataaac agtaatctct tcgttgtctg gcaaatcatc aatatgctca
     4081 agctttagat cttgatcttt aaaaatcttt tttgcttcag cttttgttac gacctcacgc
     4141 tcaaatttat tattagcggc aataatcttt ttcatttctt tttcgatctt gccaaagttg
     4201 tcacttgata ttggttttgg caaatcgaag tcgtagaaaa agccatcttc agtagcagga
     4261 ccgaagccaa tctttgttcc tgggtaaagt ttttgaactg cttcagctaa aacgtgagca
     4321 caggaatgtc tcatcgtcat caattgatct tcgttatttt ccttcttttt cgtagccata
     4381 tatcctccct aatacatagt tgaacaagcg cacaaagcca gtcaatatag at
//
LOCUS       DBNCDC010000091         4426 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_12893_length_4426_cov_7.257378, whole genome shotgun sequence.
ACCESSION   DBNCDC010000091 DBNCDC010000000
VERSION     DBNCDC010000091.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12893_length_4426_cov_7.257378"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="AC04VQ_06360"
     CDS             <1..264
                     /locus_tag="AC04VQ_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIO9553735.1"
                     /translation="VALLVEGVTKITQIEVGSVSESQALTLRKMFVAMSNDLRVIVIK
                     LADRLHNMRTLYALKEDRQIFKANETLEIFAPIAHRLGIDSIK"
     gene            424..747
                     /locus_tag="AC04VQ_06365"
                     /pseudo
     CDS             424..747
                     /locus_tag="AC04VQ_06365"
                     /inference="COORDINATES: protein motif:HMM:NF016488.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
     gene            847..1674
                     /locus_tag="AC04VQ_06370"
     CDS             847..1674
                     /locus_tag="AC04VQ_06370"
                     /inference="COORDINATES: protein motif:HMM:NF040246.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HIO9553736.1"
                     /translation="MLKSLKVDLDKAEVFVFTPEGEAKSLRAGSTPVDFAYAVHTQVG
                     HHCVGAKVDGSIVPLTYELKTGERVEILTQKSATPSRDWLNFVKTPSARSKIRQYFSK
                     QSRPDDLQAGRDILIAEMKKHGMGLSSTQMMRSIRELAGKLNFKNADELLIKIGNGKE
                     SVKNITNKLLKMVVDNGDKKESDVLTLKMQTGKDVTPKMVTNAKALTKKAAHTNSGVV
                     VKGIDDVAVRLSRCCNPVPGDAIIGFVTRGRGISVHRANCPNAKDLLKDKDRIIEVS"
     gene            1705..1995
                     /locus_tag="AC04VQ_06375"
                     /pseudo
     CDS             1705..1995
                     /locus_tag="AC04VQ_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024687.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
     gene            1995..2273
                     /locus_tag="AC04VQ_06380"
     CDS             1995..2273
                     /locus_tag="AC04VQ_06380"
                     /inference="COORDINATES: protein motif:HMM:NF014437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIO9553737.1"
                     /translation="MFNYMQRLGKSLLLPIATLPICAILLGIGYIMAPASMGAGGEMS
                     GPVYTVGLFLTTAGGALINNMGILFAIGVGIGMAKKNDGTAGVAALVS"
     gene            2535..2948
                     /locus_tag="AC04VQ_06385"
                     /pseudo
     CDS             2535..2948
                     /locus_tag="AC04VQ_06385"
                     /inference="COORDINATES: protein motif:HMM:NF014437.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            3072..3500
                     /locus_tag="AC04VQ_06390"
     CDS             3072..3500
                     /locus_tag="AC04VQ_06390"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIO9553738.1"
                     /translation="MIIPLGLAAFGVYFVVFYFYIKFFKVPIPGSGEVAAAAKKVAES
                     AKKFASKATAEKSEFAHMAEALLEAVGGKDNIESCEHCITRLRLVLKDAKIVDEDAVM
                     NCGASGIVRPAKNACQIVIGTKVQFVYDEFEKLIDNEDEE"
     gene            3504..>4426
                     /gene="nagA"
                     /locus_tag="AC04VQ_06395"
     CDS             3504..>4426
                     /gene="nagA"
                     /locus_tag="AC04VQ_06395"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIO9553739.1"
                     /translation="MILSNANIFTRDGIFETGSVVCEGDKISKLDLSSNSHDDSFDCE
                     GHYLIPGFIDIHFHGCDGHDFCEGTPEALDAISNYELKHGITSICPATMTYPKDILEN
                     AMTNAASYKNENGSEIVGINMEGPFISPQKVGAQNPKYVQSPDFEMLKQLDDSANGLI
                     KLVDIAPEVDGALEFIKKAGKNFVISIAHTNCTYSQACEAYKLGATHLTHTFNAMPPI
                     HHRSPGPIVAAAENNASAEIICDGQHVNYAAVRLAAKLFGDKLCLISDSCEATGLEDG
                     EYSLGGQAILKRGNKVVLKNNEDTIAASATNL"
BASE COUNT         1284 a          711 c          986 g         1445 t
ORIGIN      
        1 gttgcgcttc ttgttgaagg cgttacaaaa attactcaaa ttgaagttgg ttcggtttct
       61 gaaagtcaag cgctaacgct tcgtaaaatg tttgttgcaa tgagcaatga tcttcgtgtt
      121 attgttatta agcttgctga tagattgcat aatatgcgaa cgctttatgc acttaaagaa
      181 gatcggcaaa tcttcaaggc aaacgaaact cttgaaattt tcgctccaat tgctcacagg
      241 cttggaattg attcgatcaa atgagaattg gaagacttaa gtttttatta tcttgagcca
      301 aataaatata aacaaattgc tcgaatggtt gttgagtctc gtgaagaacg cgatgcttac
      361 ctaaaagaag ttattaatat tttgcaaaag gagcttaagg ataataaaat ttctgctcat
      421 ataatgggtc gcccgaagca tctttattct atttattcga agatgaaaaa taagggaaag
      481 gccttcaatg aaatttatga tttgattgct gtgcgcataa ttgttgaaga tgtaaaaact
      541 tgttatgcgg ccttaggttc tgttcatgct ctttgaagtc caattccgga gcgttttaaa
      601 gattacattg caatgccaaa agccaacatg tatcaaagtt tgcatacaag tgttattggt
      661 ccagcgggcc gtcctttgga agttcaaatt cgaaccgaag agatgcataa aacttcggaa
      721 tttggtgttg ctgcacattg gcgttataag gaaaaaagca ctgcgagcga tgctgctttg
      781 gaagagcaac ttgcttggct tcgtgagatg gttgattggc aagatgaaac tccagattct
      841 aaagaaatgc ttaaaagctt aaaggttgat ttagataaag ctgaagtttt tgtttttacc
      901 cccgaaggcg aggcgaagag tttgcgtgca ggttcaactc cagttgattt tgcttacgct
      961 gtacacactc aagttggaca tcattgtgtt ggggctaagg ttgatggaag cattgttcca
     1021 cttacatatg aacttaaaac tggcgagcgt gttgagattc ttacacaaaa gtcagctact
     1081 ccatcaagag attggctaaa ctttgtaaag acaccttcgg cacgaagcaa gattcgtcaa
     1141 tatttttcta agcagtctcg tccggatgac ttacaggctg gtagagacat cttaattgca
     1201 gagatgaaaa agcatggtat gggtctttca agcactcaga tgatgaggtc tattcgtgag
     1261 cttgcgggta aacttaattt taaaaatgcc gatgaattgc ttattaaaat tggaaacggc
     1321 aaggaaagtg tcaaaaatat caccaataaa cttttgaaga tggttgttga taatggtgac
     1381 aagaaagaga gtgatgtttt aactcttaag atgcaaacgg gtaaagatgt tactccaaag
     1441 atggttacca atgctaaagc acttactaaa aaagccgcgc acacaaatag tggtgtggtt
     1501 gtaaagggca ttgacgatgt tgctgtccgt ttgtcgcgtt gctgtaatcc tgttcctggt
     1561 gatgctatta ttggttttgt taccagggga agaggcattt cagttcacag agctaattgc
     1621 cctaatgcaa aagatttact caaagataaa gacagaatta ttgaagtttc ttgagattca
     1681 gaaaaagcta aagaaaattc ttaccgcgtt gaaattattg ttgaggctcg cgatcgaatg
     1741 ggtcttctta atgaaattac aagtgaaatt tcaaaccttg gagctaactt tacttcgttt
     1801 aatgcacatg cacacgaaga tggttataca aaaatgagct ttatgtttat attgtctgat
     1861 ttaagtcttg ctacaaaaat tgctgataac gtaaaagaaa ttgatggtgt attagcagtt
     1921 taccgagcgg ccacgtcaaa agatagacaa aattctggag aaaacgtctt agagtcagaa
     1981 aggaaaggaa agtaatgttt aactatatgc aaagattggg taagtcccta ttgttgccta
     2041 ttgcaactct tccaatttgt gcaattctac ttggtattgg ttatatcatg gctcctgctt
     2101 cgatgggtgc aggcggtgaa atgagcggtc ctgtttatac agttggttta tttttgacga
     2161 cagctggtgg cgcacttatc aataacatgg gaattttgtt tgctattggt gttggtattg
     2221 gtatggcgaa gaaaaacgat ggtactgcag gtgttgccgc attagtttcg tgacttgttt
     2281 ttactacaat cttaaatgtt gattctgttt ctaagttggt tcttgttgat agcgtagatg
     2341 aacttgcttt caaaagtgtt gctaacccgt ttattggaat tttgtcgggc ttaattggcg
     2401 caacttgctt taatggtttt ggttgaatta agctgccaga tttcttagca tttttcagtg
     2461 gtaagagatt tgttgcaatt gcctcgacag taatttcaat tattgttgct tttattttaa
     2521 tattcctttg accaatgatt tttgctggtc ttgttgcgtt tggtgacttt attactgatc
     2581 tcaaaggtat aggagttggt attttccaat ttgcaaatag gatgttaatt ccggttggtt
     2641 tgcatcatgc gctcaacaat gttttttggt ttgatactgt tggcattggt gatctcacaa
     2701 attattgggc aggtaaaact tcggcggatg ttacttgaga ccttggaatg tatatgtctg
     2761 gatttttccc accaattatg tttggtattc ctggtgcttg tcttgcgatg gttttgcttt
     2821 caaagaacag aaaaacaacg cttggtatcc ttggttcaat tgcattttgt gcatttttgt
     2881 gcggcgttac agaaccattc gaatttttgt ttatgtttat tgcgttccca ctttatatta
     2941 tttatgcagt tcttagtgga atttttggaa tcgtaactta ctttgttgga tttagggcag
     3001 gcttttcttt tagtgcaggc cttatcgatt tagtatttag ttctacattg cctgctgctc
     3061 aaaatacatg gatgattatt cctcttggat tggctgcttt tggagtttat tttgttgtat
     3121 tttatttcta tatcaaattc tttaaagttc caattccggg aagtggcgaa gttgcagcag
     3181 ccgctaagaa ggttgcagaa tctgccaaga agtttgcaag caaagcgact gcagaaaaga
     3241 gtgaatttgc tcatatggcc gaagccttgc ttgaagctgt tggcggtaaa gataacattg
     3301 aatcttgcga gcactgcatt acacgtttgc gtttggtgtt gaaggatgca aagattgttg
     3361 atgaagatgc agttatgaat tgtggtgcaa gtggaattgt tcgcccagcg aaaaatgcat
     3421 gtcagattgt tattggcaca aaggtccaat ttgtttacga tgaatttgaa aagcttatag
     3481 acaacgaaga tgaggaatag cttttgattc taagtaacgc aaacattttt acacgtgacg
     3541 gaatttttga aaccggctcc gttgtttgtg aaggcgacaa aatttcgaag cttgacctaa
     3601 gctcaaattc acatgatgat tcttttgatt gcgaaggaca ttatttaatt cccggtttta
     3661 ttgacattca ttttcatgga tgtgatggtc atgatttttg cgaaggcacg cccgaagctt
     3721 tggacgcaat ttcaaattac gaattaaagc acggcattac atctatttgt cctgctacaa
     3781 tgacatatcc gaaagatatt ttagaaaatg caatgacaaa tgctgccagt tataaaaatg
     3841 aaaatggatc agaaattgtt ggtataaata tggaaggtcc gtttattagt ccgcaaaaag
     3901 ttggcgctca aaatccaaaa tatgttcaat ctccagattt tgaaatgcta aaacaattgg
     3961 acgattcagc aaatggttta attaaattgg tcgacatagc accagaggtg gatggcgcac
     4021 ttgagtttat taagaaagcg ggaaagaact tcgttatttc gattgctcat acaaattgta
     4081 catatagcca agcttgtgag gcttataaac ttggagcaac gcacctgact cataccttta
     4141 atgctatgcc tccaatacac catcgaagtc caggaccgat tgttgcggcc gccgaaaata
     4201 atgcaagcgc tgaaattatt tgcgatgggc aacacgttaa ttatgcagct gttcgtcttg
     4261 ctgcaaaatt gtttggcgat aaattgtgtt tgatttctga tagctgtgaa gcaactggtt
     4321 tagaagatgg cgaatattcc ttgggtggtc aagcaatttt gaaacgcggc aacaaagttg
     4381 ttcttaaaaa taacgaagac acaattgctg caagtgcaac taacct
//
LOCUS       DBNCDC010000092         4233 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_13989_length_4233_cov_7.123983, whole genome shotgun sequence.
ACCESSION   DBNCDC010000092 DBNCDC010000000
VERSION     DBNCDC010000092.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13989_length_4233_cov_7.123983"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="AC04VQ_06400"
     CDS             <1..653
                     /locus_tag="AC04VQ_06400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF024575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003689 - DNA clamp loader activity
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HIO9553740.1"
                     /translation="KDIVGQDLAIKILRSELSEDRVSHAYLFCGPLGCGKLNCAKAFA
                     KSLEGDKFADLIDSGGFIDLKIYEPMGVKTYLVSQIKEIVNDTVLAPIQGKKKIYILK
                     NAESLGTAAANSFLKTLEEPESDVCFILLANNADNVLPTIASRCQIINFKQLPFDTSV
                     EIVKSNSGALEDDCKNALMLFGGNTEKAIDFCLDQNLQDYYSEIINVLNNLDSIDD"
     gene            702..1004
                     /locus_tag="AC04VQ_06405"
     CDS             702..1004
                     /locus_tag="AC04VQ_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553741.1"
                     /translation="MSVYKSSLEEKTKDLSDVLEHSAISIIEDQNKRNVGSKQRELLS
                     LFCAIIKLYYRNLIKETMQDKYIKRINCLNDFEQNLAYNISPQNFCDVVLLKIKRI"
     gene            1010..1888
                     /locus_tag="AC04VQ_06410"
     CDS             1010..1888
                     /locus_tag="AC04VQ_06410"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 domain-containing protein"
                     /protein_id="HIO9553742.1"
                     /translation="MTKIAPVKTVKANTSLWFDAGDLDIKVGQKVIVETARGIEFGTL
                     DSEIFEATDEDLKVLKSDLKPVKRIATQKDIEQADEMAQKSIDAFPIFQKHAKETNED
                     MVPISVEYLHDGRKAVFYFSAPERQDFRDLVKKLSSEFHLRVDMRQINEREKSAQVSG
                     IGFCGQELCCARLGRCPKHVSIKQAKEQGMALNPENISGMCGKLLCCLEYEYEDYKEF
                     NSRAPKLKAKISTPEGEAVVTEVNMPREYVEVKIGENEKRVKVPLDMMETDAKFAKSL
                     GNETENIRPNKIGKDA"
     gene            2241..3701
                     /locus_tag="AC04VQ_06415"
     CDS             2241..3701
                     /locus_tag="AC04VQ_06415"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIO9553743.1"
                     /translation="MSDINSKHSALLTDLYQLTMAQGYFHNNKLDMDGCFYLFFRDYP
                     FHGGYAIACGIEQIRQYIENFHFTKEDTKYLSTITGSNGKPIFTKDFLDYLEALKLEV
                     DLDCVDEGTVVFPHEPVLRVCGPILQCQIIETAILNAFNFQTLVATKASRICSAAKCP
                     VSEFGLRRAQGFAGGVWASRAAVVGGCASTSNVEAGKLFDIPVSGTHAHSWVMAFDSE
                     LEAFRAYAKEFPNNCVLLVDTYDVEQGIKNAIIVGNEMKERGEKLSAIRIDSGDLTWL
                     SKTARKMLDEGGLPDCGIILSNDLDEYSIKSIYAEGGIFTGLGVGTKLATAYDQPALG
                     GVYKLSATKMHDEKVWNPHMKVSESSQKTTIPGVLNVRRYIDDNGKLAGDMIYDISMG
                     VDKTCTIVDPMDILRQKKLGEKNYTDLLKPLFKKGKLVGKTFDTMHAQENAKQGLETL
                     DETQKRTLNPHTYPVGIERTLNEYRTKMVAKLRGIS"
     gene            3701..4099
                     /locus_tag="AC04VQ_06420"
     CDS             3701..4099
                     /locus_tag="AC04VQ_06420"
                     /inference="COORDINATES: protein motif:HMM:NF013431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIO9553744.1"
                     /translation="MITKSGLDSSNFEFRSAGVVSEELYMSYTAQVPTGLFTLTNNKG
                     MEVCITNVGARIVSIMVADRLYNFRDVVLGFDSLKGYIDHDQRHSNSHGAIVGRFANR
                     IANGEFEIDGKKYKLPQNNGTNCLHGGPYG"
BASE COUNT         1429 a          647 c          872 g         1285 t
ORIGIN      
        1 ttaaagatat tgttggtcaa gatttggcca taaaaattct tcgttcagag cttagcgaag
       61 atagagtttc gcatgcgtat ttattttgtg gtccacttgg ttgtgggaag ctgaattgcg
      121 cgaaggcttt tgctaaatct ttggaaggcg ataaattcgc tgatcttatt gatagtggcg
      181 gttttatcga tttaaaaatt tatgaaccta tgggtgttaa aacttatctt gttagtcaaa
      241 ttaaagaaat tgttaacgat acagttttag cgcctattca aggaaaaaag aaaatttata
      301 ttttaaaaaa cgccgaaagt cttggaacag ctgctgcaaa ttcttttctt aaaacattgg
      361 aagagcctga aagtgatgta tgttttattc ttcttgcaaa taatgctgat aatgttttgc
      421 caacaattgc ttcgcgttgt caaattatta attttaagca actgccgttt gatacttcgg
      481 ttgaaattgt taaatcaaat tctggtgcat tagaagatga ttgcaaaaat gctttaatgt
      541 tgtttggtgg aaatactgaa aaggcaattg atttttgcct cgaccaaaac ttgcaagatt
      601 attattccga aataattaat gtgttaaata accttgattc aattgatgat tgagattgca
      661 ttttaagaag ttcagatatt gtcaataagc aaaatgaaat tgtgtcggtt tataaaagta
      721 gtctggaaga aaaaacaaaa gatttaagcg atgttttaga gcattcggca atttcaatta
      781 ttgaagatca aaacaaaaga aatgttggtt caaaacaaag ggaattattg tccttgtttt
      841 gcgcaattat taagctgtat tacagaaatc taattaaaga aacaatgcag gataaataca
      901 ttaaaaggat aaactgcctc aatgattttg aacaaaattt agcgtataat ataagcccac
      961 aaaatttttg tgatgttgtg ttattaaaga taaaaaggat atagaagata tgacaaaaat
     1021 tgctcctgta aagactgtta aagctaatac ttcgctttgg tttgatgccg gcgatttaga
     1081 tattaaagtt ggtcaaaaag ttattgttga aacagctcga ggaattgagt ttggaacgct
     1141 cgattcagag attttcgaag caacagacga agaccttaaa gttttaaaaa gcgatcttaa
     1201 gccagtcaag cgcattgcta ctcaaaaaga tattgagcaa gctgacgaaa tggcacaaaa
     1261 aagtattgat gcttttccaa tttttcaaaa gcatgcaaaa gaaaccaacg aagacatggt
     1321 tccaatctcg gttgaatatt tacacgatgg aagaaaagct gtgttttatt tttcggcacc
     1381 agagcgtcaa gattttcgtg atttagtcaa aaaactatca agcgaatttc atcttcgtgt
     1441 tgatatgaga caaattaacg aacgtgaaaa aagcgctcaa gtttcaggaa ttggattttg
     1501 tggtcaagaa ttgtgttgtg cacgtcttgg tagatgtcca aaacatgttt cgataaagca
     1561 agcaaaagag cagggtatgg cacttaatcc tgaaaatatt tcgggaatgt gtggtaagct
     1621 tttgtgctgt ttggaatatg aatacgaaga ttacaaagaa tttaattctc gtgccccaaa
     1681 acttaaagca aaaatttcta ctccagaagg cgaagctgtt gtcacagaag ttaatatgcc
     1741 tcgtgaatat gttgaagtta aaattggcga aaacgaaaag cgtgtaaaag ttcctctgga
     1801 catgatggaa acagatgcta aatttgcaaa aagtcttgga aatgaaacag agaatatccg
     1861 tccgaataaa attggtaaag atgcttgaga aaaagttcaa gaagaaaatt cagtaatttc
     1921 cttagaaagt gtttacacaa cgcaaacctt aaaaggttct gaaaaacttg cgactggtgt
     1981 tgttaaaact cttccaaatg ttaagcgtcg tggaagcaag accttcggcg gtgacaaaac
     2041 cgaagaaaac aaacctcgtc caagaaaaaa taacagaaga aataatcaaa aacgttctaa
     2101 aaacgcagtt ccaaaaggca atgtatacga aagcaataaa actcaaactg tccgtaggcg
     2161 cacaagaaag ctagattcgt ctgctgcaag gcccggacaa aactcttctg gtttaagaaa
     2221 ataaaatagg aagagagaaa atgagtgaca ttaattctaa gcattcggca cttttaactg
     2281 atttatatca gttaacaatg gctcaaggtt attttcacaa caataaactt gatatggatg
     2341 gatgttttta tcttttcttt agagattatc ctttccatgg cggttatgct attgcttgcg
     2401 gtattgaaca aattagacaa tacatagaaa actttcattt tacaaaggaa gacacaaagt
     2461 atctatcaac aattactggt tctaatggca agccaatttt tacaaaagat tttcttgatt
     2521 atttagaagc gctaaagctt gaagttgatt tagattgtgt tgacgaaggc acggtcgtgt
     2581 ttccacatga acctgttctt cgcgtttgtg gtccaatttt acaatgccaa ataattgaaa
     2641 ctgcaatatt aaacgcattt aattttcaaa cccttgttgc aacaaaggct tcgcgaattt
     2701 gctctgcggc aaagtgtcct gtaagtgaat ttggacttcg tcgtgcgcaa ggttttgcag
     2761 ggggagtttg ggcttcgcga gctgctgttg ttggtggatg cgcttcaact tcgaatgtag
     2821 aagctggaaa gctctttgac attcctgttt ctggaactca cgctcacagc tgggttatgg
     2881 ccttcgatag cgaactggaa gccttccgcg cttatgcaaa agaatttcca aacaattgtg
     2941 ttttgcttgt ggatacttat gatgttgagc aaggaattaa aaacgcaatt attgtcggaa
     3001 atgaaatgaa ggagcgaggc gaaaagcttt ctgcaattcg aattgattct ggcgatttaa
     3061 cttggctaag caagactgct cgaaaaatgt tggacgaagg cggccttccc gattgtggaa
     3121 tcattctttc taatgatttg gatgaatatt caatcaaatc aatttatgca gaaggtggaa
     3181 tttttactgg acttggagtt ggcacaaagc ttgctactgc atatgaccaa cccgcattgg
     3241 gtggagttta taaactgtca gcaacaaaaa tgcatgatga aaaagtttgg aaccctcaca
     3301 tgaaagtttc tgaaagttct caaaaaacta caattccagg cgtgctaaac gttcgtcgat
     3361 acattgatga taatggcaag cttgcagggg atatgattta cgatatttct atgggcgttg
     3421 ataaaacttg cacaattgtt gaccctatgg atattcttcg tcaaaagaaa ttgggtgaaa
     3481 aaaattacac tgatttgcta aagccgttat ttaaaaaagg aaaacttgta ggtaaaactt
     3541 ttgatacaat gcatgcacag gaaaatgcta aacaaggttt agaaactctt gatgaaactc
     3601 agaagagaac gctgaatcct cacacttatc cagtaggaat tgagcgcact cttaatgaat
     3661 atagaacaaa gatggtggct aagttgcgag gtatttcata atgattacga aaagtggatt
     3721 agacagttca aattttgaat ttagatcagc tggagttgtt agcgaagaac tttatatgtc
     3781 ttacactgca caagttccga ccggactatt cactctaact aataataaag gtatggaagt
     3841 ttgcattacg aatgtaggtg ccagaattgt ttctattatg gttgcagata ggttatataa
     3901 ctttcgcgat gtagttcttg gatttgattc cctaaagggt tatatcgatc atgaccaaag
     3961 acatagtaat tctcatggag ctattgtcgg acgttttgcg aatagaattg ctaatggaga
     4021 gtttgaaatt gacgggaaga aatataaact tccgcaaaat aatggaacca actgccttca
     4081 tggtggccct tatggatgag cttttcaaac atttagcgtt attgaacaca cagaaagtaa
     4141 gcttgtatta aatttaaaat ctcctgatat ggatatgggc tttccaggaa acgttgattt
     4201 ttatgttcat tatgaagtct ttgatgataa ttc
//
LOCUS       DBNCDC010000093         4193 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_14258_length_4193_cov_6.978009, whole genome shotgun sequence.
ACCESSION   DBNCDC010000093 DBNCDC010000000
VERSION     DBNCDC010000093.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14258_length_4193_cov_6.978009"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="AC04VQ_06425"
     CDS             <1..546
                     /locus_tag="AC04VQ_06425"
                     /inference="COORDINATES: protein motif:HMM:NF020892.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIO9553745.1"
                     /translation="EFNSCWPMQYHFVGKDIIRFHCVIWPAMLMAAGLPQPKCIFGHG
                     FLLTKGEKMSKSKGNAVMPQDIINVFGLDAYRYYFMSDVQFGADGSISLERMVQVYNA
                     DLANTWGNLCSRVFNMTNKYFDGKVPDADASAENPMKEIVDGLYKKYDEAMERVDFTA
                     ATKAALELAERVNHYVEESEP"
     gene            586..729
                     /locus_tag="AC04VQ_06430"
     CDS             586..729
                     /locus_tag="AC04VQ_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553746.1"
                     /translation="MLYNMLESIRIISLYMAPFMPVTSKEVYEALGQGNVEEVTDIKS
                     ATA"
     gene            819..1706
                     /locus_tag="AC04VQ_06435"
                     /pseudo
     CDS             819..1706
                     /locus_tag="AC04VQ_06435"
                     /inference="COORDINATES: protein motif:HMM:NF013213.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
     gene            1706..2584
                     /gene="rsmA"
                     /locus_tag="AC04VQ_06440"
     CDS             1706..2584
                     /gene="rsmA"
                     /locus_tag="AC04VQ_06440"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIO9553747.1"
                     /translation="MAGSETSPLANIAIAREVIATYSLQAKKSFGQNFLINDAIVRKT
                     IELADVKAGDYVTEIGPGYGTLTVALVRAGANVLAVEKDAKMINALYDNLEKYCEEGK
                     DKIEVLRQDALRLDFGAYERRLDGKSVKMVSNLPYNVAAPIVITYFQKLPNMSQATVM
                     VQKEIADRMVAKPGNKNYGAYTVKLAMFADYAGKFPVSRSNFMPQPRVDSTVINLVRH
                     DRIKPKIANTACVIADAAFAHRRKTIANSVKEYFCDDTIMLEKIAKAFEKSNMDMSLR
                     AEAISPQDYAIIAGNL"
     gene            2594..3574
                     /locus_tag="AC04VQ_06445"
     CDS             2594..3574
                     /locus_tag="AC04VQ_06445"
                     /inference="COORDINATES: protein motif:HMM:NF019342.7"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIO9553748.1"
                     /translation="MNKSNAKFSLSIIGIIMLFAALLLCVSCMKPAKVIGVAWDNKDN
                     NENTVLALQTANTEVKAEVLPLVKYEGFTYDGDKVTGDCIDKYGMLTNAAAEQLKAKP
                     YDKTNVAEVVKGHDAIVFSGGSDVPPSLYKNPQPYYDLSEDPGFNPTRDISDYILMSY
                     CLDNNIKCMGICRGMQLYAVVAGAEMVQDLPVYYASQGVPYNNEHRKSGEINGKDVRG
                     NHPVYVTDKSSYLYDCVKTDVIDKPYSSHHQAVLSVDPAHSKVTAVGRVNGLETIEAL
                     ERTDSDTFSFFIQFHPEHALARWVNDKPEKESFMSYETSLSFFKYFIDHI"
     gene            3649..4137
                     /locus_tag="AC04VQ_06450"
     CDS             3649..4137
                     /locus_tag="AC04VQ_06450"
                     /inference="COORDINATES: protein motif:HMM:NF018016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HIO9553749.1"
                     /translation="MDLQHQAKIVDGIREKMQGKINERIFVKVDLGRSRIHENVGTLV
                     QTHQSVFIVELEQGRGRISKQSFQYADILTGKVEIYQNNKAMFGPFNIVPDAPLVEPS
                     KQPRKKKRLRPSKPIEQRIRLNSSQVAMLVERIKAESEANKKDGKEIVLELSKNFTDK
                     KD"
BASE COUNT         1225 a          873 c          997 g         1098 t
ORIGIN      
        1 gagttcaaca gttgttggcc gatgcagtat cacttcgttg gtaaagacat tattcgcttc
       61 cactgcgtta tttggccagc gatgcttatg gcggctggac ttccgcagcc taagtgtatt
      121 tttggccacg gatttttgct aaccaagggc gagaaaatgt cgaagagcaa gggaaatgct
      181 gttatgcctc aagacataat taatgttttt ggcctggatg cttatcgcta ttattttatg
      241 agcgacgttc aatttggcgc cgatggaagc atcagtctcg agcgcatggt tcaagtttac
      301 aacgctgatc ttgcaaacac ttggggcaat ttgtgctcgc gcgtctttaa catgacaaac
      361 aaatatttcg atggcaaggt gccggatgca gatgcttcgg ctgaaaatcc tatgaaggaa
      421 atcgttgacg gcttgtataa aaaatatgac gaagccatgg agcgcgtcga ctttacggct
      481 gccacaaaag cagctttgga gctggctgag cgtgttaacc actacgttga ggaaagcgag
      541 ccgtgaaacc tcgctaagtc cgaagataaa accgagcttg caaatgtgct ctataacatg
      601 cttgaatcta tccgaatcat ttcgctttat atggctccat ttatgcctgt gacgtccaag
      661 gaagtttatg aggctttagg gcagggtaac gttgaagaag ttacagacat caagagcgca
      721 actgcttgag gtcagctaaa accaggcact gatattaaaa tgacagacgc attattccca
      781 agactcaaag aagatgaagt cgatttttca atcgaataat gtccgaagat agacacacag
      841 atttctacga aaagcgcaaa aagggcaagt ttcgccttgt agagccgcct gacacgagct
      901 ttccggttgc tgatacccat tgtcattttg aaatgttcga agacccagcg tggaacttcg
      961 ttagatgcgc tgtgcataat gtaaatttca ttgcttgtgt tcttgatagc accgatgatg
     1021 gtcccgaagg catggccaaa gtcgaggctg catacgaaga agcgaagaac ttacttccag
     1081 ctggaactaa tcttccgcaa ttgaaataca tcgttggcac tcatcctcac catgcgaaga
     1141 agtgggatga tgctcaagaa aagaacttgc gagagatgct taaaaaccca aaggtctctt
     1201 gcgtgggcga gattggtctt gattaccact atgatttctc gcccaagaaa gaccaaattg
     1261 atgtctttaa acagcagcta aaaattgctg acgagcttgg gtttcctgtt tcgcttcatc
     1321 ttcgtgaggc tcatgatgac gctcttaaaa tttttgagga agtaggattt tgcaagcacg
     1381 gaacccttct tcattgtttt aatttaggac ccgaagacct aaagccttgg gtggaagctg
     1441 gttgctatat tgcaattggc gggcctgtca ctttcaaaaa attcgattga ctccgagaag
     1501 cagtgaagtt catcccgcaa gataaattgc taacagaaac cgacgcgcca tttatgactc
     1561 cagagccaat gcgaggcatg acttgttggc cggaccacgt aatttttgtg gcagacatgc
     1621 tttgtaaaac tttagggcaa gaaagcaatc gcgaagaatt ttacgcgcgc atgttccaaa
     1681 acgcgctcga tctattggac agataatggc tggtagcgag acatctccac ttgcaaacat
     1741 cgcaattgcg cgtgaagtaa ttgctactta tagtctgcaa gcgaaaaaat ccttcgggca
     1801 aaactttttg attaatgatg caattgtgcg aaagacaatt gagcttgccg atgtcaaagc
     1861 aggtgattac gtgaccgaaa tcggtcctgg atatggcacg ctaaccgttg ctcttgttcg
     1921 tgctggcgca aatgttttgg cagttgagaa agatgcgaag atgatcaacg cgctttatga
     1981 caaccttgag aaatattgcg aagaaggcaa ggacaaaatt gaggtgcttc gccaagatgc
     2041 gcttcgcctt gacttcggcg cttatgagcg gcggcttgat ggcaaaagtg tgaagatggt
     2101 ttcgaacctt ccatataatg ttgccgcgcc aatcgtgatt acatattttc agaagttgcc
     2161 aaacatgagc caggctaccg tgatggttca aaaagaaatt gcggatagaa tggtagcgaa
     2221 gccaggcaac aagaactacg gcgcttacac cgtaaagctt gcgatgttcg ctgattatgc
     2281 tggcaaattt ccagttagtc gcagcaactt tatgccgcag ccaagggttg atagcacggt
     2341 cattaacctt gttcgtcatg acagaataaa gccaaaaatc gcaaacactg cctgcgttat
     2401 agctgacgct gctttcgcgc accgccgcaa aaccatcgca aattcagtca aggaatattt
     2461 ttgcgacgac actataatgc ttgagaaaat cgcgaaggca ttcgaaaagt caaacatgga
     2521 tatgtcgctt cgtgccgaag caatcagccc gcaggattat gctataattg ctggcaactt
     2581 ataaggagga atcatgaaca aatcaaacgc aaaatttagt ttatcaatta ttggaatcat
     2641 tatgcttttc gcggctttgc tgctatgtgt ttcatgcatg aagccggcga aagtcattgg
     2701 cgttgcgtgg gataataagg acaacaacga aaatactgtg cttgctcttc aaacagcaaa
     2761 caccgaagta aaggccgaag ttttgccatt ggttaaatat gaaggcttca catatgatgg
     2821 tgataaggta acaggcgact gtattgacaa gtatggcatg cttacaaatg ctgctgctga
     2881 gcagctaaaa gccaagcctt atgacaagac gaatgttgcc gaagttgtca aaggccacga
     2941 cgcaatagtc ttttctggag gctcggatgt tccgccttcg ctttataaaa atccccagcc
     3001 atattatgac ctaagcgaag accctggttt taacccaact cgcgacattt cggactacat
     3061 cttgatgtca tactgcctgg acaacaatat taaatgcatg ggaatttgca gaggcatgca
     3121 gctctatgct gttgttgctg gcgctgaaat ggttcaggac ctgccggtat attatgcaag
     3181 ccagggagtt ccgtacaata atgaacacag aaagtccggc gaaattaacg gcaaggacgt
     3241 tcgaggaaac cacccagttt atgtgacaga caagagcagt tatctttacg attgtgtaaa
     3301 aaccgatgta atcgataagc cttattcgag ccatcaccag gcagttttga gcgttgaccc
     3361 agcgcattct aaggtaacag cggttggtcg tgttaacggt cttgaaacta tcgaggctct
     3421 tgagcgaact gacagtgaca cttttagctt ttttattcaa ttccatcctg aacacgccct
     3481 tgcgcgatgg gtaaatgaca agccagaaaa agaatctttc atgtcttacg agacatcact
     3541 aagtttcttt aaatacttta ttgaccatat ctagtctcaa aacttgacaa gtggggtttt
     3601 aggagagtat aatacgccgc tggtaaaatc aaattttgag gtgataaaat ggatcttcag
     3661 catcaagcaa aaattgttga tggcattcgt gaaaaaatgc aaggtaagat taatgagcgc
     3721 atttttgtta aggttgacct gggccgttcc cgcattcatg agaatgttgg caccttggtt
     3781 caaacccatc aaagcgtttt tatagttgaa cttgaacagg gcagaggccg aatttcgaag
     3841 caaagtttcc aatatgcaga tatccttact ggcaaggttg aaatctacca gaacaataag
     3901 gctatgtttg gacctttcaa tattgttcca gatgctccat tggttgagcc ttcgaagcag
     3961 cctcgcaaga agaaacgtct tcgtccgtct aaacctattg agcagcgcat tcgccttaac
     4021 tcaagccagg ttgcaatgct ggttgaacgc attaaggcag aaagcgaagc gaacaaaaaa
     4081 gacggcaaag agattgtttt agaacttagc aaaaacttca ctgataagaa ggattagcaa
     4141 tacgtgggga tgtagctcag ttgggagagc gcagcgttcg caacgctgag gtc
//
LOCUS       DBNCDC010000094         4098 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_14829_length_4098_cov_7.892159, whole genome shotgun sequence.
ACCESSION   DBNCDC010000094 DBNCDC010000000
VERSION     DBNCDC010000094.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14829_length_4098_cov_7.892159"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="AC04VQ_06455"
     CDS             complement(<1..299)
                     /locus_tag="AC04VQ_06455"
                     /inference="COORDINATES: protein motif:HMM:NF023809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HIO9553750.1"
                     /translation="MNLKIGSDAKIIFKTNDKEIEYSGDVVEEARTKAVTKDEVADHI
                     CRTGQTPFRIDKLEINLDENVGIGFSTLHHLRADALTLLEEKILKTDRPLLEKRKP"
     gene            complement(495..1571)
                     /locus_tag="AC04VQ_06460"
     CDS             complement(495..1571)
                     /locus_tag="AC04VQ_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013316.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIO9553751.1"
                     /translation="MTSNDGHIELLAPAGNFKALQAAVCGGANAVYLGLVHFNARRGA
                     DNFNLKNLKAACDYAHLRGVKIYLTLNIVVFESEFNTAMQMAYDAANAGVDAFIVQDL
                     GIARCVVKEIPHVPLHISTQMNIHDEDGLRAVADIGAKRVCLGRECSLDTIKRLCDLG
                     RELNVEIETFGHGALCICYSGQCLMSSMIGGRSANRGTCAQACRLNYELHEGKASLGV
                     RRYDEDDRRGEYLLSPKDLCTIEILPKLQDAGVSALKIEGRIKSADYVNEVVNVYRHA
                     LDRLHKNSEDYQVTQKEMTRLQEAFSRGFTTAYIEGKRGNEIMSYKRPNNRGINIGRV
                     SELNSRKIAITSKEIINIGDVLEV"
     gene            1635..1832
                     /locus_tag="AC04VQ_06465"
     CDS             1635..1832
                     /locus_tag="AC04VQ_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553752.1"
                     /translation="MNKFIEKIQEPMMKIADKFNTITFLCVIRDAFFICMPIIIFGSL
                     VTIVANFPFLNYIIGEELAAD"
     gene            2004..2144
                     /locus_tag="AC04VQ_06470"
     CDS             2004..2144
                     /locus_tag="AC04VQ_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553753.1"
                     /translation="MAVDADGEQTGKIVAKAITALTTEQLGAKGMFVAMFASFGASAL
                     FC"
     gene            2163..2807
                     /locus_tag="AC04VQ_06475"
                     /pseudo
     CDS             2163..2807
                     /locus_tag="AC04VQ_06475"
                     /inference="COORDINATES: protein motif:HMM:NF014437.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            2880..3134
                     /locus_tag="AC04VQ_06480"
     CDS             2880..3134
                     /locus_tag="AC04VQ_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553754.1"
                     /translation="MAIDGALWLPFLLTIDKRMCREESRTIATNALNIIIAATEAKQE
                     AGEKALAEEEKAKKKTTKKPAPKKTTAKKTTTKKKTTTKK"
     gene            3154..3456
                     /locus_tag="AC04VQ_06485"
                     /pseudo
     CDS             3154..3456
                     /locus_tag="AC04VQ_06485"
                     /inference="COORDINATES: protein motif:HMM:NF014368.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3471..3857
                     /locus_tag="AC04VQ_06490"
                     /pseudo
     CDS             3471..3857
                     /locus_tag="AC04VQ_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012457.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
BASE COUNT         1243 a          798 c          773 g         1284 t
ORIGIN      
        1 ggtttgcgtt tttcaagaag aggtctgtct gtctttagaa ttttttcttc aagtaaagtc
       61 aaagcatccg cgcgaagatg atgcaatgtt gaaaagccaa taccaacatt ttcatccaag
      121 ttgatttcaa gtttgtcaat cctaaatgga gtttggcctg ttctacaaat gtggtctgct
      181 acctcatctt ttgtgacggc tttagtgcga gcttcctcaa ctacatcgcc actatattca
      241 atttctttgt cattagtttt gaatataatt tttgcgtcac taccaatttt taaattcaat
      301 atacattcaa caggaacctt tggagcaaac tggtcaactt caaaactaca ttcggcagac
      361 ttgacacgaa aaactctgtc accttttcgg atatttttta aagaataatc atttttatta
      421 tttaaaaact caacgccttt gtctttaaac tttgttgtct tatcaacctt taaagttgat
      481 cttccacggt ttgttcaaac ttcaaggaca tcaccaatat ttataatctc ttttgaagta
      541 atagcaatct ttctagaatt aagttcgcta acacggccaa tatttatgcc gcgattattc
      601 gggcgcttat aactcatgat ttcattgcct cgcttgcctt caatataagc ggttgtaaaa
      661 cctcgcgaaa aagcttcttg caaacgagtc atttctttct gcgttacttg atagtcttcc
      721 gaatttttat gcaatctgtc caatgcatgt ctataaacat ttacaacttc atttacatag
      781 tctgcacttt ttattctgcc ctcaattttt agagccgaaa ctcccgcatc ttgcaacttt
      841 ggcaaaattt caatcgtgca taaatctttt ggagacagca aatactcgcc gcgcctatca
      901 tcttcatcat atctgcgaac acccaggctg gccttgcctt cgtggagttc ataattaagt
      961 ctgcaagctt gagcgcaagt tccacgattt gcagagcgcc cacctatcat cgaagacatc
     1021 aaacactgac ccgaataaca aatgcaaagc gcaccgtgac caaaagtttc aatctcaaca
     1081 tttaattcgc gtcctaaatc acacaaacgt ttaattgtat cgagagaaca ttcacggccg
     1141 aggcaaactc gcttggctcc aatatcggcc acagcgcgaa gtccgtcttc gtcatgaata
     1201 ttcatttgcg ttgaaatatg aagcggaaca tgcggaattt ctttcacgac gcatcgtgca
     1261 attcccaggt cctgaacaat aaaagcgtcc accccagcgt ttgcagcatc atatgccatt
     1321 tgcattgcag tgttaaattc gctctcaaaa actacaatgt tcaacgttag ataaattttc
     1381 acaccacgaa gatgagcgta atcgcaagcg gccttcaaat tcttcaaatt aaaattatca
     1441 gcacctcggc gcgcattaaa atgaacaagc cccaaataaa cagcatttgc tccaccacaa
     1501 accgcggcct gcagagcctt aaaatttccg gccggagcaa gtagttctat atgtccatca
     1561 tttgaagtca cgacccatat tataaacaaa aattatggta aaattatcgt ttgtgttttt
     1621 ttgaggaggc ttaaatgaat aagttcattg aaaaaattca agaaccaatg atgaaaattg
     1681 cggataagtt taatacgatt acttttcttt gtgtaattcg tgatgcattc tttatttgta
     1741 tgccaatcat tatttttggt tctcttgtaa caattgttgc taacttcccg tttctaaatt
     1801 acataattgg cgaggaactt gcggcagact gaaaagcact gctgggacca gcaagtaatg
     1861 caacaattaa tgcactttca ttgttcttat gcattggaat tggttattac tatgcaagat
     1921 ataaagaggt tgacagcctt tatggtgcag ctgtttgtgt ttgcgcattt atggttttag
     1981 caccttggtg aagtggcgat gtaatggctg ttgacgcaga tggagagcaa acagggaaaa
     2041 ttgttgccaa agcaatcact gcgcttacaa ctgaacaact tggagcaaaa ggaatgtttg
     2101 ttgcgatgtt tgcttccttt ggcgcttcgg ctttgttttg ctgactttca aagaaaaacc
     2161 tacaaattaa aatgcctgag caagttcctc ctggagtcgc aaaaagtttt gcttcattaa
     2221 tccctgcaat gatttgctta gttgtatttt tagttgttcg agtactgttc actttcacac
     2281 tttgagaaga catatttaca tttgtttatg aatgtgttca agctccactg gtagcaatgg
     2341 gttctggaat tggtcctgcc attgttgcaa tattctttgt ccagttcttc tggttcttcg
     2401 gccttcatgg acaaactatt atcaattctt gcatggatcc aatttggaac acattgatga
     2461 tggaaaacta caatgcgtat actgcaggtg atccacttcc ttacataatt acaaaaccat
     2521 ttatggaaac atttactgtt ggacttggag gaagcggtgc aaccttaatt gttattgttc
     2581 taattattat atttgcaaaa tcaaaacagt ttaaaggtat tggtaaactt gcccttgctc
     2641 ctggaatttt taacgtaaac gaaccattta tttttggtat gccaattatc tttaacccat
     2701 tgtttataat tccatggata ataattccaa ttgttaattt acttgttgct tattatgcaa
     2761 tgtggtttga tcttgtccct tacactactg gtgttcttct gccgtgaaca acaccagtat
     2821 ttttgagtgg tttcttagct acaaattcat gacagggtgc agtgttgcaa gcggttcaaa
     2881 tggcaataga tggagcgcta tggcttccat tcttacttac aattgacaag cgaatgtgcc
     2941 gtgaagaaag cagaacaatt gcaactaatg cgcttaacat aattattgcc gcaaccgaag
     3001 caaagcaaga agccggcgaa aaggcactgg ctgaagaaga aaaagctaag aaaaaaacaa
     3061 cgaagaagcc agcacctaag aaaactacag ccaaaaaaac tactacaaaa aagaaaacta
     3121 ctactaagaa ataaatagat aaaggagaaa tttatgaata ttttattagc atgctcagcc
     3181 ggaatgtcaa caagtttact tgttaacaag atgattgatg cctccaaagc tgatggccgc
     3241 aaagacaaaa tttgagccgt tggtcaagct gaacttgacg aagcaatgaa agactgtgat
     3301 gttttattgc ttggtccaca gatgcgtttt ttggctacaa aaatccagcc taaagctgac
     3361 gaacttggga ttcctatgga ggtaattcct gcaactctat atggaagagt tgatggaaag
     3421 gcagtcttgg acttcgctga tgaattagct tcgtaagtta ggtttataat atgaaattaa
     3481 attataagtt ccccgaaaat ttcttatggg gtgccgccag tagcggacct caatccgaag
     3541 gtgccgcaaa cattgacggt aaaggcgaat ctatttgaga ctactgatat tcgacaaatc
     3601 ctgaattgtt ttttaacgaa gttggaccgt ccgacacttc aactttttat aaaaattaca
     3661 aagaagatat caagttgatg aaaaaggttg gtttaaattc ttttaggaca agtatccagt
     3721 ggtcgcgact tatgccagac aaaacaagca aggttaactt tgaagctgtt aagttttata
     3781 acaacgttat tgacgaaatg cttgcaaatg gcatattgcc aattatgaat ttacatcact
     3841 ttgacatgcc aatgtgagcg caaaagcttg gtggttgaga aagcagagaa gttgtaaatt
     3901 attatgttca atatgcgaag gcttgtttta gattgtttgg agatcgcgtt aagttttgaa
     3961 caactttcaa cgaacctgtt gtaactccag aaatgggata tttaggaaat tggcactatc
     4021 cttgcattgt tgatggtaag cgagcagttc aagttgctta tcatgaaatt cttgcaagcg
     4081 caattgcaat tagcgaat
//
LOCUS       DBNCDC010000095         4042 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_15214_length_4042_cov_7.748182, whole genome shotgun sequence.
ACCESSION   DBNCDC010000095 DBNCDC010000000
VERSION     DBNCDC010000095.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15214_length_4042_cov_7.748182"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            92..226
                     /locus_tag="AC04VQ_06495"
     CDS             92..226
                     /locus_tag="AC04VQ_06495"
                     /inference="COORDINATES: protein motif:HMM:NF014690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HIO9553755.1"
                     /translation="MIFGTFCSYIAAIGVILGHIFSVFHKFKAGKGVAPSIGVVFAVD
                     "
     gene            complement(163..408)
                     /locus_tag="AC04VQ_06500"
     CDS             complement(163..408)
                     /locus_tag="AC04VQ_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553756.1"
                     /translation="MFLRWLKTINIQIIIAKIKNSLTKNQDTIGKTIAELIEPTLTFR
                     VIKTNINPKPKHINASLQSTANTTPIDGATPLPALNL"
     gene            481..1110
                     /locus_tag="AC04VQ_06505"
     CDS             481..1110
                     /locus_tag="AC04VQ_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015539036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HIO9553757.1"
                     /translation="MAREKFDYFRAFHDLTGKAIQEANCLIRIFENFNGTDELYDDMQ
                     AVHEIEHAGDEINHQIFKNVARDFITPIDREDIISLAGNLDEILDLIEDAIQHIYMYD
                     VHKLQPDAITFAKLVKKSCVALHECTEIIYKRKNIRKEISEKIVKVNDAEEEADLLYM
                     DVIRKLYTKEKDKPVKIMVWSMIYHNMEECCDACEHASDVLRTVLLKNS"
     gene            complement(1107..1397)
                     /locus_tag="AC04VQ_06510"
     CDS             complement(1107..1397)
                     /locus_tag="AC04VQ_06510"
                     /inference="COORDINATES: protein motif:HMM:NF015734.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIO9553758.1"
                     /translation="MTMILDGEEVVDFPKVDDPTYTQKSEKIKDPNDDEELANAPNVV
                     GMTIQEAKATLAEYNLTIYYVYSPTVASGVVMSQSVQNGRIVLNVSKGPAPS"
     gene            complement(1515..2339)
                     /locus_tag="AC04VQ_06515"
     CDS             complement(1515..2339)
                     /locus_tag="AC04VQ_06515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIO9553759.1"
                     /translation="METYSDAEIFEGGFQIYTTLNIKHQEACEAGCASQNKKLEEGAE
                     SVAVTVDPSNGYITGMVGGDDYSVHQYNIATSKGRPTGSSFKMFTLATAIEQGYDPFS
                     YTVDCSGPMKVGTVRIENYGNASYGTKTIQGATAVSSNTGFVRLQQKVETQNVINMAQ
                     KLGIKKAELPIVTTLTLGVADINPLEMASAFATVAAGGIYHEPVSILRIETASGDEIF
                     NYDPQENPDNGQRVIDEKVAGAVTKVLQTVFTQGTATSAKPNSKQPIAGKTGTSED"
     gene            complement(2400..3218)
                     /locus_tag="AC04VQ_06520"
     CDS             complement(2400..3218)
                     /locus_tag="AC04VQ_06520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIO9553760.1"
                     /translation="MALVQRLKIHEEMPIKQIIKVLMIIFAALFAFGLVFLFIYTIQI
                     QNKLENYDDSVVFNLAQNSKIYAADGQTVLAELQIENREPVSSLNEISPNVINASIAT
                     EDARFYQHKGVDLFALFRAAGVIFSGGSTQGGSTITMQLVRNTILSKEAQTMTLERKI
                     SEMILANRIEEIYDKETILLMYLNTINYGDRCYGIKAASRHYFSILPDQLNLVQAATL
                     VGIPQSPVYLSPTENPDACKLRRNTVLRRMLEEHMITDEEFNTASATPIDLAVS"
     gene            complement(3365..4042)
                     /locus_tag="AC04VQ_06525"
                     /pseudo
     CDS             complement(3365..4042)
                     /locus_tag="AC04VQ_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012457.7"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
BASE COUNT         1167 a          716 c          730 g         1429 t
ORIGIN      
        1 taatgctgtc cgagttcttg gatgaggctt ggggctttgt gtcttcggcc ttgatctgct
       61 taaaggattt cttactactt ttcttgcttg aattatattt ggaacatttt gttcttatat
      121 agcagcaata ggtgtaattc ttggacatat tttttcggta tttcacaaat ttaaggcagg
      181 caaaggtgtt gctccatcaa ttggtgttgt gtttgctgtt gattgaaggc ttgcgttaat
      241 atgtttaggt tttggattaa tattagtttt aattacccga aaggtaagcg ttggctcaat
      301 tagttcagct atagttttgc caatcgtgtc ttgattttta gttaaagaat tttttatttt
      361 cgcaataata atttgtatgt taatagtttt tagccaccgc aaaaacattg tccgtttata
      421 taaaggcgaa gaaaaaccac tcttttaatt tgatataatt aaagagtcaa aggagtaata
      481 atggcaagag aaaagtttga ttattttaga gcttttcatg atttaacagg aaaagctatc
      541 caagaggcaa attgtctaat tcgtattttt gagaatttta atggcactga tgagctctat
      601 gatgatatgc aagctgttca tgaaattgaa catgcgggcg atgagattaa tcatcaaatt
      661 ttcaagaacg ttgctcgcga ttttattact ccaattgatc gtgaagatat tatttctttg
      721 gctggaaatt tagatgagat tttggatttg attgaagatg caattcaaca tatttatatg
      781 tatgatgttc acaagttgca acctgacgct ataacttttg cgaaacttgt gaaaaaatct
      841 tgtgttgcgc ttcatgaatg cacggaaatt atttataaaa gaaaaaacat tcgtaaggaa
      901 attagtgaaa agatcgttaa ggttaacgat gccgaagaag aagctgatct tttatatatg
      961 gatgtaattc gtaagttata caccaaagaa aaagataaac ctgtaaagat tatggtatgg
     1021 tccatgattt atcacaatat ggaagaatgc tgcgatgcgt gtgagcatgc ttcggacgtt
     1081 cttcgtactg ttttgcttaa gaattcctaa gaaggcgcag ggcctttaga aacattcaag
     1141 actattcgtc cgttttggac actttgactc ataacaactc cgcttgctac tgttggagaa
     1201 taaacgtaat atattgttaa gttgtattca gcaagagttg cttttgcttc ttgaattgtc
     1261 attccaacaa catttggagc atttgcaagt tcttcgtcat cgtttggatc tttaatcttt
     1321 tcagattttt gtgtatatgt tgggtcatca actttaggga agtcaacaac ttcttcgcca
     1381 tcaagtatca tggtcataat atctctaaat agtccattgc aagatacgct acttgaagtt
     1441 ggagaataat cgcgtttgcc aattcatgtt gccaaaacaa cttttggtgt atagccaatt
     1501 aaagtgtgat ctcttcaatc ctccgaagtt ccagtcttgc ccgcaatagg ttgcttgctg
     1561 tttggcttag cactggttgc tgttccttga gtaaaaactg tttgtaaaac ttttgtaacg
     1621 gcgcctgcaa ccttttcgtc aataactctc tggccattgt cagggttttc ttgtgggtca
     1681 taattaaata tttcatcgcc gctggctgtt tcaatacgaa gaattgaaac tggttcgtgg
     1741 tatattccac cagcagcaac agttgcaaat gcgcttgcca tttcaagtgg attaatgtca
     1801 gcaactccca atgtaagtgt tgtaacaatt ggaagttctg cttttttaat tcctaatttt
     1861 tgtgccatat taattacgtt ttgagtttca actttttgtt gaagacgaac aaatccagtg
     1921 tttgaagaaa ctgccgttgc tccttgaatt gtttttgttc cataacttgc gtttccataa
     1981 ttttctattc gaacagttcc aactttcatt ggaccactgc aatctacagt gtaggaaaat
     2041 gggtcatagc cttgctctat tgctgttgct aaagtaaaca ttttaaacga agacccagtt
     2101 ggccttcctt ttgacgtagc aatgttatat tggtgaaccg aatagtcatc accaccaacc
     2161 attcccgtaa tatatccatt gcttggatca actgtaacgg caacgctttc ggctccttct
     2221 tcgagctttt tattttgact ggcgcaacca gcctcgcaag cttcttgatg tttgatattc
     2281 aaagttgtgt aaatttggaa gccaccttca aaaatttcgg catctgaata tgtttccaaa
     2341 atttgctcgc gaacataatt tgtaaagtat gggtatttat aattttcact tggatcattt
     2401 cagctgacag caagatcgat tggagtagcg ctcgcggtgt taaattcttc gtcagttatc
     2461 atatgctctt caagcatacg acgaagaaca gtattccttc gaagcttaca agcgtctggg
     2521 ttttcggtag gagacaaata caccggagat tgcggaatac ctaccaaagt tgccgcttga
     2581 acaagattta gttggtcagg caaaattgaa aaataatgcc tggaagctgc ctttatgcca
     2641 taacatctat ctccataatt gattgtgttt aaatacatta acaaaattgt ttctttgtca
     2701 tatatctctt caattctgtt ggctaaaatc atttcagaaa tttttcgctc aagggtcatt
     2761 gtttgagctt cttttgaaag tattgtgttg cgcacaagtt gcatagtaat tgtgcttccg
     2821 ccctgagtgc tgcctccgct aaaaattact ccggcagctc taaacaacgc aaacaaatca
     2881 acgcccttgt gctgataaaa tcttgcatct tctgttgcaa ttgaagcgtt aattacattt
     2941 ggagaaattt catttaacga agacacaggc tctcgatttt caatttgtaa ttcagctaaa
     3001 acagtttgac cgtccgcagc atatattttc gaattttgtg caagattaaa aacaactgag
     3061 tcatcatagt tttcaagttt attttgaatt tgaattgtgt aaataaataa gaagactaat
     3121 ccaaatgcaa acaaggctgc aaaaataatc atcaaaactt taattatttg cttgatgggc
     3181 atttcttcgt gtatttttaa tctttgaact aaagccataa ttaattaaat ccgttatttt
     3241 tcgaaacctt cttaaatcat tcgccacttt ttttaattgt gcgtttttgt gtttttaaat
     3301 caagagacac aaagccgtat ctgtttttat aggcgttgcg tcaacttcaa ttatcaataa
     3361 aggttcacat atgataacca aagcagtttg cgccagcttc gataccttta tgtaaacatc
     3421 ttagatgatc tttgacaaaa tctatcctgt ataaatcctc cacctggcct ttttcgttaa
     3481 taaaacgctc ttcgccttca acacccattc cgttttctga aataaagaac ggaatgttgc
     3541 cgtaattttt catgatgttg tcgcaaatat cgtagatgcc ttcttcgtaa atttctcagc
     3601 ctctgtattt atttattttt gccattggcc atacatagtt ttcgaaataa atttctggag
     3661 taaaaacatc cacgtctaat ttttctgttc tcgcttgagc gcgacgagga caataataat
     3721 ttactcccaa aaagtctaca gtgttttctt ttatggtctc aatatctttt tgttcatatt
     3781 ttggaagcgc accattttct ttaagcaaac taattagttc ttgtggatat tcacctaaaa
     3841 ctgaaggatc caaaaatgca cgattgtaaa ataaatcttg aatatttgcg gctttaacat
     3901 tttctttttc ataatctttt ggatatgttg gcattaagtt caaaataatt ccaatagaac
     3961 catctttgct gcaatccatc ttatgatatt cgctaattgc aattgcgctt gcaagaattt
     4021 catgataagc aacttgaact gc
//
LOCUS       DBNCDC010000096         4017 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_15379_length_4017_cov_6.108026, whole genome shotgun sequence.
ACCESSION   DBNCDC010000096 DBNCDC010000000
VERSION     DBNCDC010000096.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15379_length_4017_cov_6.108026"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="AC04VQ_06530"
     CDS             complement(<1..269)
                     /locus_tag="AC04VQ_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIO9553761.1"
                     /translation="MNEVYDIVIIGAGVSGCACARELSRYDAKILVVDKEEDACCGTS
                     KANSAIVHAGFDAAEGSLMARLNVQGNLMMPKLASDLDIPFIQNG"
     gene            complement(483..629)
                     /locus_tag="AC04VQ_06535"
     CDS             complement(483..629)
                     /locus_tag="AC04VQ_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553762.1"
                     /translation="MIGDSSIIKKNSYVDISDDNKTIDFYDPDTGELVCKYVVELTGY
                     SSIS"
     gene            complement(735..1478)
                     /locus_tag="AC04VQ_06540"
     CDS             complement(735..1478)
                     /locus_tag="AC04VQ_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553763.1"
                     /translation="MNLTGSVDLEEVTVYDKTIYKGALKDPYGTKISTDLTVKFETEE
                     GDSFAIKTSDGNYNISVYAGTKGKFTFDAGDEYVATEIDLITASIKDFSPTTLDVVVC
                     KKNESATISGIVTPPKEGVEINFVYPSGLKNISYTDAEGKYSINVVPNIDGELFIIDH
                     SENVTFASPITSPYQGGTIEKNVTMNAATVTVDIDSGSVSDYSIPIGWTRISNNTFSK
                     KYVIDSTFKCILKGWKEVIVPIDTLYFNG"
     gene            complement(1869..2663)
                     /locus_tag="AC04VQ_06545"
     CDS             complement(1869..2663)
                     /locus_tag="AC04VQ_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIO9553764.1"
                     /translation="MPVVGATVSLFDENGNLVEKALTDNQGKYLFKNLPEDFKGYVNI
                     ETKPGSSEGLAGASALISVDGLTKVEIPSLPKSILTGQASDSQPGTTVKFTTEDGRVY
                     ITKTDAQGKYRLPVSANTKGNLNFDAGAGVITNNYNNITTPEPGSTSEYDIYITPASE
                     TSKITGAILPRVSGNTVQFIDSKGLVYTAITEDNGRYDLIIPANEKGTFYVLANKNTT
                     TYLYMEDMLSPDAGKSTAATRDASVSKVVRVNLDPQTTVGGYMPEG"
     gene            complement(2874..3635)
                     /locus_tag="AC04VQ_06550"
     CDS             complement(2874..3635)
                     /locus_tag="AC04VQ_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553765.1"
                     /translation="MNTFIEFVAKNPDTPGGNTAQTFDIAGIALFTLFAIVAVFGIVS
                     ILTSKGSIKTISSNLATKVANVFNVKILYAIFAVLAALLLIFAVMFGNKQAYADSATQ
                     TKVADKVTAYVDINTGEISIEPGFISSTDTNIVLLNSVDLSLCEGILDGNCNWKISSD
                     DKILFEGKAGEAHALTPPFQIYQDSEVPITFSTDMNLETAKSLIGKKVASVSFDLTQK
                     DAVYYTASVQLNSGTVTAEGEAALKADGWSESGGS"
     gene            complement(3792..>4017)
                     /locus_tag="AC04VQ_06555"
     CDS             complement(3792..>4017)
                     /locus_tag="AC04VQ_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012455.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HIO9553766.1"
                     /translation="LIVGGVIATIVMCMGGVTAACLNPARDWGPRLVYTLMPMGKKGD
                     KEWKYGIIVAGLAPFIGAFLAIGLNAIMPW"
BASE COUNT         1261 a          810 c          680 g         1266 t
ORIGIN      
        1 cttccgttct ggataaatgg aatgtccaag tcagaggcta actttggcat catcaaattc
       61 ccttggacat tcaatcgagc catcaagctt ccttcggcag catcgaaacc agcgtgaaca
      121 atagcagaat tcgccttcga agttccgcag caggcgtcct cttctttatc aacaaccaga
      181 atttttgcat catagcgcga aagttcccgg gcacatgcgc acccggaaac ccccgcacca
      241 ataatcacta tgtcataaac ttcattcacg tttactaact aatctttata tgcaaacaaa
      301 tcttcaataa aatcaaaaga taaaaactca tcgaaatctg gttcatcaat ttcatcatca
      361 ggcgaaaatt cattattatc ggggttgtca tttgaaatta atgaacaaga tggaataaat
      421 tttactggag caataagaca gctggtaaca taaaattcaa ataacttctc agactcaaat
      481 gatcacgaaa ttgacgaata gcccgttaat tcaacaacgt atttacaaac gagttcgcct
      541 gtgtcaggat cataaaaatc aatagtctta ttgtcgtctg aaatatcgac ataagaattt
      601 ttctttataa tagaagaatc cccaatcaca aaagatccct taacatcatc tggaaaatcg
      661 ccaacaagaa aatagttagt atataaaact ttatctgtag tgttttgaga aagtgtactt
      721 tcaacatcaa ctgatcatcc attaaaatac aatgtatcga taggtacaat tacctctttc
      781 catcctttta atatacattt aaaagtacta tcaataacat acttttttga aaatgtatta
      841 tttgaaattc ttgtccaacc aattggaata ctataatcac taacacttcc agaatcaata
      901 tcgacagtaa cagtggcagc attcatggtt acattttttt caattgtgcc cccttgataa
      961 ggagaagtaa tcggcgaggc aaaagtaacg ttttcgctat ggtcaataat gaatagctca
     1021 ccatcaatgt taggcacaac attaattgaa tatttacctt cagcgtctgt ataagaaata
     1081 tttttaagtc cacttgggta aacaaaattt atttcgacac cttctttagg tggtgtcact
     1141 ataccactaa tagtggctga ttcatttttc ttacaaacaa caacatcaag agttgttggg
     1201 ctaaaatctt taattgatgc ggtaattaag tcaatttctg ttgctacata ttcatctccg
     1261 gcatcaaatg taaactttcc tttagttcca gcataaacac taatattgta attgccgtcg
     1321 ctcgttttta tggcgaatga atcgccttct tcagtttcaa atttaacagt tagatcggtg
     1381 gaaatttttg tcccataagg atctttaagt gctcctttat aaatagtttt atcataaacg
     1441 gtaacttctt ccaaatcaac tgaaccagtt aaattcatat taatatctga agaaatatca
     1501 tttaagcaat atccactttt accctcaatc ttaatttgag cgttaggaat tggggcatca
     1561 agaaaagtag caaaattacc attattatcg gatagaactg aagtaactaa ttttcctgtt
     1621 gcgtcgtata tactaattga gacgcctctt tcaaaatgcc cattggcatc cttaatacgt
     1681 cccttaattt gtatggtatc tttagaaact caaactggta tatatttatt accagacaaa
     1741 gaccagtttt taaatacttg atctgtcgga gaagtaatct tcgttatgtc ccactcatca
     1801 agaattaaat tatcattagt tgtagaagca aattccttca tgtattgatt tggatcggta
     1861 tctttatttc agccttctgg catataacca cctacagttg tttgagggtc aagattaaca
     1921 cgaacaactt ttgacacgga tgcatccctt gttgccgctg tagacttccc agcatctgga
     1981 gaaagcatat cttccatata taaatatgtg gtcgtgttct tgtttgcaag tacataaaaa
     2041 gtacctttct cgttcgctgg aataattaag tcatacctac cattatcttc agtaatcgcg
     2101 gtataaacta atcccttgct atcaataaat tgaaccgtat taccagaaac tcttggaaga
     2161 attgctccgg taatttttga agtttcggaa gctggagtta tataaatatc gtattccgaa
     2221 gtgcttccgg gttcaggagt tgtaatgttg ttgtagttgt tagttattac accagctcca
     2281 gcatcgaaat taagattacc ttttgtattt gctgaaactg ggagtctata cttgccttgt
     2341 gcatctgttt ttgtaatata gactctaccg tcttcagtag tgaacttgac agtggttccg
     2401 ggttgagaat cacttgcttg acctgttaat atagatttcg gcagcgaagg aatttcaacc
     2461 ttagttaggc catctacact gataagcgcc gaagctccgg caagaccttc gctacttcca
     2521 ggtttagttt caatatttac atatccttta aaatcttctg gaagattttt gaataaatat
     2581 ttaccttgat tatcggttag agccttctca acaagatttc cattttcatc gaacaatgaa
     2641 actgttgcac caactactgg cacaccttcg tctaaaattt ttgcttcaat aataggtttc
     2701 ttgactcatt gagcagtata agttgtactc gacaaaaatc tttctcctgc tggaacaata
     2761 tttcaattat caaatatata agtatttgga gcttgaacct tttcactagc tattaattca
     2821 ttccacgttg gaagaataac actatggcga attccctcaa taaaattctt agatcatgat
     2881 ccaccagatt ctgaccatcc gtccgcttta agcgcggctt cgccctctgc agtaaccgtt
     2941 ccagaattaa gctgtacgct agctgtataa taaacagcat ctttttgagt aaggtcaaat
     3001 gaaacagaag ctaccttttt accaataagc gattttgctg tctccaaatt catatctgtc
     3061 gagaaagtga ttgggacttc gctgtcttga tatatttgga aaggtggcgt tagggcatga
     3121 gcttcgccgg cttttccttc aaacaaaatc ttgtcatcac tcgaaatttt ccaattgcaa
     3181 ttgccatcaa gaatgccctc gcacaaagac aggtcaactg aattgagcaa aacaatattt
     3241 gtgtcagtcg aggatataaa tcctggttcg atgctaattt cgccagtgtt gatgtcgaca
     3301 tatgctgtaa ctttgtcagc aacttttgtc tgagttgccg aatcggcata tgcctgttta
     3361 ttgccgaaca ttactgcaaa tatcaataat aatgcagcca aaacagcaaa aatagcatac
     3421 aggattttta cgttaaaaac gtttgcaact tttgttgcga gattgcttga aattgtttta
     3481 atgctacctt ttgaagttag aatagagaca attccaaaaa cggcgacaat agcaaaaagc
     3541 gtaaataaag caattcctgc aatgtcaaaa gtctgtgctg tgtttccacc tggagtatca
     3601 gggttcttag caacaaattc aataaatgtg ttcatcacac tcccctttcg aataatttgc
     3661 aaataatatt ttaccaaaga acaggataac tgtccttata tattaaaaag acactttaag
     3721 tttgatttct tttggaaatc gagtgattcc ccggagcaaa tcgagtgtgg gccccgggga
     3781 aatttagtat tttaccaagg catgatggcg ttgaggccga ttgcgaggaa agcgccgatg
     3841 aatggagcta aaccagcaac aataatgccg tacttccatt ccttgtcacc ctttttgccc
     3901 ataggcatta gtgtgtaaac aagacgagga ccccaatcac gagcagggtt caagcaagca
     3961 gcagttacac cacccatgca cattacgatg gttgcgataa ctccaccaac aatcaac
//
LOCUS       DBNCDC010000097         3893 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_16277_length_3893_cov_7.674310, whole genome shotgun sequence.
ACCESSION   DBNCDC010000097 DBNCDC010000000
VERSION     DBNCDC010000097.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16277_length_3893_cov_7.674310"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="AC04VQ_06560"
     CDS             <1..313
                     /locus_tag="AC04VQ_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome C oxidase Cbb3"
                     /protein_id="HIO9553767.1"
                     /translation="KDINEEVSSINLKMNKNQGAQVFDVKINFTDSGHSTYFDTTEYT
                     VGIPKIEMSTNSKRSQFFTIHPTGKVKDMKMQIYGEDLNYPLYIDALIINAHYPFDFV
                     V"
     gene            344..526
                     /locus_tag="AC04VQ_06565"
     CDS             344..526
                     /locus_tag="AC04VQ_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553768.1"
                     /translation="MIYAFRPRSSVYKIKLVDRPLYTRAVICLTIFIETILLSAYLLM
                     GSNLVGVATSNYNYGD"
     gene            656..1042
                     /locus_tag="AC04VQ_06570"
     CDS             656..1042
                     /locus_tag="AC04VQ_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553769.1"
                     /translation="MDDPYDKGSRDEMEKQTGQKYLFDVAYYDGHYYVYFGVVPVLLF
                     YLPFYLIAGSNFPTAIGVLIMAILFIIGLSVLLDRFARYHFERVSLGLYLLLQIPLIM
                     CSGILYLCKFPTFYSLPLACGVAFAV"
     gene            1115..1294
                     /locus_tag="AC04VQ_06575"
     CDS             1115..1294
                     /locus_tag="AC04VQ_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553770.1"
                     /translation="MALIAGCRPQIMLIAAVAIPLFWRHFITNARTTGLKTKKGWIEL
                     ACLAAPFVIVGMGLM"
     gene            1346..1873
                     /locus_tag="AC04VQ_06580"
     CDS             1346..1873
                     /locus_tag="AC04VQ_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553771.1"
                     /translation="MTMNDMTQRGLQVSRVLPALFAFFIQPPNVTGVFPFINPVIFET
                     TYLGQTIKEVTFGGVLVCLPVLWILFFAPPLLKFRRKVRQTNTIMGVIIVLIVVAVLI
                     AILDAQMAGILQRYYADFSFMLLIAAVLLAFIANEKISLASKNLRSIATGVMLTLVCV
                     SVLYSLLTCFVVETG"
     gene            1947..2207
                     /locus_tag="AC04VQ_06585"
     CDS             1947..2207
                     /locus_tag="AC04VQ_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553772.1"
                     /translation="MIKKNYLFLFATLCISIVMILIFASACSASVPKVDTIYKTQEQF
                     QKEFAVDTYAPNKQITGDYDESHAAKCNNGTFVGYAQNDVNV"
     gene            2214..2918
                     /locus_tag="AC04VQ_06590"
                     /pseudo
     CDS             2214..2918
                     /locus_tag="AC04VQ_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012363.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
     gene            2952..3740
                     /locus_tag="AC04VQ_06595"
     CDS             2952..3740
                     /locus_tag="AC04VQ_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HIO9553773.1"
                     /translation="MRDGKIVPADPVAAYEDGAAKDILMLQGATTNEFEYYIAVFHWV
                     YDIYDKACTKLREACVNSGNDEFKAAYNEYVQALKDLGVEDDKIDHEFASDKALEFIN
                     TYQAECQSKNGKDGYAYAFDIGYDGDYITLGAAHAVDCHYLFGNFDGNHAKGNKAEVD
                     ASRTFQRMISNFCKNGDPSTDKVKWDKYNSNSRTKMILREEDFHQINNYQGARVDALC
                     KMSKSSDVFRYAGRMGDLINAVAEDDPESFFYLILGELIAQNSK"
BASE COUNT         1108 a          697 c          829 g         1259 t
ORIGIN      
        1 taaagatatc aacgaagaag tttcaagcat caacctaaag atgaataaaa atcaaggtgc
       61 gcaggtcttt gatgtgaaaa ttaatttcac tgatagtgga cattcgacgt attttgacac
      121 gaccgaatac accgttggca ttcctaaaat tgaaatgtca acaaattcca aacgaagtca
      181 atttttcaca attcatccga cgggcaaagt caaagatatg aagatgcaaa tttatggcga
      241 agacctaaac tatcctctct atattgacgc tctcattatt aacgcgcatt atccttttga
      301 ttttgttgtg tgaagattca ttgcagcgtt tgcaatttta tttttgattt atgctttcag
      361 gccgcgttca agtgtttata aaattaagct tgtggacagg cctctttata cccgtgctgt
      421 aatttgtcta acaattttta tcgaaacaat tttgcttagc gcttaccttt tgatgggctc
      481 aaatttagtt ggagttgcaa cgtcaaatta taactatggc gattgagacg gtgtaagtcc
      541 aattaatact tttgaagttg ccggcgataa tgctcaacaa tatgcagaac ttgcgaagtc
      601 ctttactcaa ggtaagcttt atcttgaaca ggaaccacca gattacttaa agactttgga
      661 tgacccttat gataagggta gtcgcgatga aatggaaaag caaaccgggc agaagtatct
      721 cttcgatgtt gcatattacg acggacatta ttatgtttac ttcggagttg ttccggttct
      781 tttattttat ttgcctttct atttaattgc gggctcaaac ttcccaactg caattggcgt
      841 tttgataatg gccatattat ttattattgg tttgagcgtt cttttggacc gctttgccag
      901 gtatcacttt gagcgtgtta gtcttggact ttatttattg cttcaaattc ctcttattat
      961 gtgcagtgga attttgtatt tgtgtaaatt cccaacattt tattcattgc cgcttgcgtg
     1021 tggtgtggcc ttcgctgttt gagcacttta cttttgaatg cgaggcagag catccacaaa
     1081 accttacggt tgattcattg ctggaagttt ttgcatggca cttatcgcag gttgcaggcc
     1141 acaaattatg ttgattgccg cagttgcaat tcctctcttc tggcgacatt tcattacgaa
     1201 cgctcgcacg accggcctca agacgaagaa gggttggatc gagcttgctt gtttagccgc
     1261 tccatttgta attgttggta tgggactcat gtgatataac tacgcgcgct tcggaagtgt
     1321 cacaaacttt ggtgcaaatt acaatttgac aatgaatgat atgactcagc gaggtcttca
     1381 agtgtcacga gttttgccgg cgctttttgc cttcttcatt cagccgccaa atgtgactgg
     1441 agtgttccca tttataaatc cagtaatttt tgaaactaca tatttgggtc aaactattaa
     1501 agaagtaaca tttggtggcg tgttggtctg ccttccagtt ttgtggatcc ttttcttcgc
     1561 ccctccgctt ctaaagttcc gaagaaaagt ccgccagaca aacaccatta tgggagtaat
     1621 tattgtcctc attgttgtgg cagttctaat tgctattttg gatgctcaaa tggctggaat
     1681 tttgcaacgc tattatgcgg acttcagctt tatgttgctg attgctgctg tcctcctcgc
     1741 gtttattgct aatgaaaaga tttctcttgc atcgaagaat ttgcgaagca tcgcaacagg
     1801 tgttatgctt actcttgttt gtgtgagtgt tttatacagt ttgctgactt gcttcgttgt
     1861 ggaaactggt tgatacggtg acgtctacaa ttgagcctac gtcgatgtaa ttaaatcctt
     1921 tctgttctga acttaggcgg tgttaaatga ttaagaagaa ttatttgttc ttatttgcaa
     1981 ctttatgtat ttctattgta atgatattaa tttttgcgtc ggcttgttcg gcgagcgtgc
     2041 ctaaagttga cactatttat aaaactcagg aacaatttca aaaagaattt gcagttgata
     2101 cttatgctcc aaataagcaa atcactggtg attacgatga gagtcatgct gctaaatgca
     2161 ataacgggac attcgttgga tatgctcaaa atgatgtaaa tgtttgaaag ggcattccat
     2221 atgcgaagca acctttgggt gaacttcgtt ttaagcaggc tcaagctcca gatgatagcg
     2281 actcagttta cgaagctcag cactttggca gtgcatgtat gcaagctcca agcccgggcg
     2341 agaaggcttc gctttatcat caaggtgaag attgccttaa cttaaatatt tggtcaagta
     2401 atttaatgaa gtctaataaa cctattttgg tttacattca tggtggcggt tgaattagtg
     2461 gaggaagtgc tgaccctctt tataatggat acaactttgc aaattacaat ccagatatta
     2521 tgcttgtaac tattaattat cgtacgggca tgatggggat gattgacctt tcttctttcg
     2581 agggctctca agaatatgat acttctataa ataacagcct acttgatatg gttcaagcct
     2641 taaagtggtt aaagcaaaac ggtgctgctt ttggtggtga cgtaaataac attacaattt
     2701 gtggtgaaag tgctggtggt ggtgctgttt cgacgctttg cataatggat gcagctaagg
     2761 gccttttcaa caaagcaatt cctatgagtg gtggcgttga tcaaattcaa aagaaggata
     2821 acaccacagc attgccaact caatttaaaa aggactttaa tatttctagc gttaagcagg
     2881 ctacagaaat tccttttgaa aaactttgtg aatggtgaaa cgtaaatgga gaaagtgttg
     2941 cgcatcatat aattcgtgat ggaaaaattg ttccagctga ccctgttgcg gcttacgaag
     3001 acggggccgc caaagacatc ttgatgctac aaggtgcaac tactaatgaa tttgaatatt
     3061 acattgcggt cttccattgg gtttatgata tttacgacaa ggcttgcact aagttacgcg
     3121 aagcttgcgt aaattctggc aatgatgaat ttaaggcagc ttacaacgaa tatgtacagg
     3181 cgcttaaaga tttaggggtt gaagatgaca aaatcgatca tgagtttgct agtgataagg
     3241 cgcttgaatt tataaatacg taccaggcag aatgtcaatc taaaaatggc aaggacggat
     3301 atgcttatgc cttcgacatt ggttacgatg gggattatat tactttaggt gcggcacatg
     3361 cagtggattg tcactacctc ttcggtaatt ttgatggaaa tcatgcaaag ggcaacaaag
     3421 ctgaagttga tgcttcgcga acctttcagc gaatgatttc taacttttgc aaaaatggtg
     3481 acccatcaac agataaagta aagtgggata aatacaattc aaattctcgc actaaaatga
     3541 ttttacgaga agaagatttt catcaaatta acaattatca aggcgctaga gttgatgcgc
     3601 tttgcaagat gtctaaatct agcgatgttt ttagatacgc gggaagaatg ggtgatttaa
     3661 taaacgccgt tgccgaagat gacccagaga gttttttcta tcttatcctt ggtgaactaa
     3721 ttgctcaaaa ctcaaaataa attttagttg ccaaagaagt taaagttaga cattaagagt
     3781 tcaacgccta aacctgcggc agcgcacaaa ataaaagtga gaacacatgt taaaattatc
     3841 ttgcccgtag tatattcgtc tttctttctt aattgtacaa aggtgacaac agt
//
LOCUS       DBNCDC010000098         3881 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_16368_length_3881_cov_8.616832, whole genome shotgun sequence.
ACCESSION   DBNCDC010000098 DBNCDC010000000
VERSION     DBNCDC010000098.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16368_length_3881_cov_8.616832"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="AC04VQ_06600"
     CDS             <1..463
                     /locus_tag="AC04VQ_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553774.1"
                     /translation="HSYIQDWLEQNTDMDEDIPEFDAAIEQGRKSIEQSRKSIEAKKE
                     SIEEMRTEVYDKIAEIRRQEEEIAKHAAAIDVKKRAVRRVKRRRRIKESDSTISNIAE
                     AVITQSATDDKNIPGADTVGQTDSFDPIKENGNTGKIPKVEPKHKKTTNKK"
     gene            464..1609
                     /gene="brnQ"
                     /locus_tag="AC04VQ_06605"
                     /pseudo
     CDS             464..1609
                     /gene="brnQ"
                     /locus_tag="AC04VQ_06605"
                     /inference="COORDINATES: protein motif:HMM:NF017347.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
     gene            1824..3071
                     /gene="pyk"
                     /locus_tag="AC04VQ_06610"
     CDS             1824..3071
                     /gene="pyk"
                     /locus_tag="AC04VQ_06610"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIO9553775.1"
                     /translation="MKDLRKTKIICTIGPASSSEDMVKKLVEAGMNCARINCSHGSAK
                     DRQPVVATLKKVRKDMDVPLAILLDTKGPEFRIKTFSEGKVFVEDGSTFTFTTDDVEG
                     DQTKVSVTYEKLCDDLEPGDIVLVNDGLVKFEVETIEGHNINCKTLIGGELSNNKSMN
                     FPGKDLTVEFLSETDKQDLLFAIKNDLNFIACSFVSNAQDIKSVRDFMHENGGDKISL
                     IAKIENQSGIDNLEEICKHCDGVMVARGDLGVEIDFDKLPAVQKQIIKECGRLGKLAI
                     TSTEMLESMIEKPRPTRAEVSDVANAVYDGTAALMLSGETAAGKYPVKAVKAMSKIAK
                     STEEKVHYRKRFLYAKPVVENRTVDAIAHATCAIAIDVNAKGIVNVTASGFTSELISN
                     FRPDVNVCAITINQHEYYKLALY"
     gene            3117..3254
                     /locus_tag="AC04VQ_06615"
     CDS             3117..3254
                     /locus_tag="AC04VQ_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553776.1"
                     /translation="MEVLNLTKKYAEEVLGLEAGEKFVLNSGFQDEEIGYTNALMVEM
                     I"
     gene            3255..>3881
                     /locus_tag="AC04VQ_06620"
     CDS             3255..>3881
                     /locus_tag="AC04VQ_06620"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIO9553777.1"
                     /translation="MLFTNIKYIDENFEVIDNAYVGVVNDVIEYVGTQKPNDIDKYGE
                     VYDGNNKLLMSGFYNVHAHAAAVILRSYADKQPLNEWLNDWIFPFEAKMTDEDYYWST
                     LLGMAEMARFGIVSCSDMYFGGEARVKALLESNMKCNTCEAIVSFDDIHFSEHPLYKV
                     SMEMIDKYNGANNGKIITDLCIHGEYTTKETICREIGQMAKDKNLIIHS"
BASE COUNT         1209 a          533 c          804 g         1335 t
ORIGIN      
        1 gcattcttat attcaagatt ggcttgaaca aaatacagat atggatgaag atattccaga
       61 gtttgacgct gcgattgaac aaggaagaaa atctattgag cagtcgcgta aatctatcga
      121 ggctaagaaa gaatctatcg aagaaatgcg caccgaagtt tacgacaaga ttgctgagat
      181 tcgccgtcaa gaagaggaaa ttgcgaagca tgccgctgca attgatgtta agaagcgtgc
      241 tgttcgtcgt gttaagagac gtcgaagaat caaggaaagt gattcgacaa tttcaaatat
      301 tgctgaagct gtaattactc aatctgctac tgacgataaa aatattccag gagcagatac
      361 ggttggacaa acagattcgt ttgatccaat taaagaaaat ggaaatacag gtaagatacc
      421 aaaggttgaa ccgaaacata aaaaaactac aaacaaaaaa taaatgaata ataaattaag
      481 ccgtaaacaa atattaattg ttgcctccac actttttgga atgatttttg gtgcaggaaa
      541 tttaattttt cctgttcata tggggcaact tgccggtagt aattttattt ctgcaacact
      601 tggttttgta attactggtg ttggcattcc aattttagga ataattgcta ttggttatag
      661 tgaatctaaa ggtctttttt ctctaacaag taaagttggt aagtattttg catatattta
      721 taccatgata ttatatttag caataggacc tatgtgcgtt atgccaagaa gtgcttctat
      781 tgcttatggg tctggattgt ctacaattat tggtgacagc tttaatcaag atattgcttt
      841 tttgattttt tcaataatat tttttgctat aactttattt tttagcttaa agccttctgg
      901 tattacaact tctgttggaa aaataattaa tcctatcttt ttaatatttt tgtttttctt
      961 aatatttgtt gctctcttga atccgcttgc aaatgttttt gaagtacaac caacagcgga
     1021 atatgaaacg cagcctttct tctatggttt tattcaaggc tatggaacta tggatgcttt
     1081 ggcgggcctt gccttcggaa ttattttgat agacattgtt aaacgtatgg gtagtaaaga
     1141 gccaagtgca attacaaaaa atattatgat accaagtgtt ttaactggtt gtttgatgat
     1201 ggcaatttat acccttacta ttattatggg tgtagaaagt agaggcgctt ttgatatttc
     1261 aagtaatgga agtattgcgt taagccaaat ttcgaataat tattttggaa tttgaggaga
     1321 cattgtttta actaccattg ttactttagg tgcgcttaaa acatctattg gattggttac
     1381 aagttgttca gctacttttt atgaaatgct tccaggtaaa aaaattagtt atcatgcttg
     1441 gtgtgttatt tgtgttgtaa tgggaattag tatttctaac attggtcttg ataacatctt
     1501 aacttttact cagccaatat taagcttttt gtgtcctctt gcaattgtta ttattattat
     1561 ggcttttgtt tgtaagcgtt ttaattattc aaaagtcatt tatcaatgaa caatatatat
     1621 tacttgtatt cctgccttat tcgatttgtt gcgaactttg cctccacaag taattgaagc
     1681 attaaacctg ggcggcgtga tagactttgg tcgagcgatt ttcccattta tggatattgg
     1741 ttttgcttga gtaattcctg ccgcaattgg atttttgatt ggtataatta tttatgctta
     1801 taaaaaatca agtaggggca aaaatgaaag acttaagaaa aactaaaatt atttgcacaa
     1861 taggtcctgc ttcgtcttcg gaagatatgg ttaaaaagct tgttgaagct gggatgaatt
     1921 gcgctcgtat taattgctcc cacggatcgg cgaaggatag gcaacctgta gttgcaactc
     1981 ttaaaaaagt ccgaaaagat atggatgttc cacttgcaat tctgcttgat actaagggtc
     2041 ctgaatttcg tattaaaaca tttagcgaag gaaaagtatt tgttgaagat ggatccacat
     2101 ttacttttac taccgatgat gttgaaggcg atcaaacaaa ggtttctgta acttatgaaa
     2161 agctttgcga tgaccttgag cctggtgaca ttgttttagt taatgatgga cttgtaaaat
     2221 ttgaagttga aacaattgaa ggtcataata ttaattgcaa gacacttatt ggcggagaac
     2281 ttagcaataa taagagtatg aattttcctg gtaaggattt aactgttgaa tttttaagtg
     2341 agacagacaa gcaggatttg ctcttcgcta ttaaaaatga tttgaacttt attgcatgtt
     2401 cttttgtttc aaacgctcaa gacataaaaa gcgttcgtga ttttatgcat gaaaatggtg
     2461 gagataaaat ttcgctgatt gcgaaaattg aaaatcaatc cggcattgat aatttggaag
     2521 aaatttgtaa gcactgcgat ggcgttatgg ttgctcgagg ggatcttggt gttgaaattg
     2581 attttgataa gttgcctgca gttcaaaaac aaattattaa agaatgcgga cgtcttggaa
     2641 agcttgcaat tacaagtacc gaaatgcttg aatctatgat tgaaaagcct cgcccaactc
     2701 gcgctgaagt ttccgatgta gccaatgctg tttatgatgg aactgctgcg ttgatgcttt
     2761 ctggtgaaac tgcggctgga aaatatccag tgaaagctgt taaagcgatg tcaaagattg
     2821 caaagtctac tgaagaaaaa gttcattata ggaaaagatt tttatatgca aagcctgttg
     2881 ttgaaaatag aactgttgat gctattgctc atgcaacttg tgctattgca attgatgtta
     2941 atgctaaagg cattgttaat gtgacagcaa gcggatttac ttcggagcta atttctaatt
     3001 ttaggcccga tgtaaatgtt tgtgccataa ctattaatca acatgaatat tataagctgg
     3061 ctttatattg aggtatttat ccagttcttg aaaataaacc aactgatatt tctaatttgg
     3121 aagttttaaa tttgacaaaa aaatatgctg aagaagttct tggacttgaa gctggcgaga
     3181 aatttgtttt gaattcgggt tttcaagatg aagaaattgg ttatacaaat gcattaatgg
     3241 tggagatgat ctaagtgctt tttactaaca ttaaatatat tgatgaaaac tttgaagtta
     3301 ttgataatgc ttatgttggt gttgttaatg atgttattga atatgtggga acgcaaaagc
     3361 ctaatgatat tgataagtat ggtgaagttt atgatggcaa caataaattg ttaatgtctg
     3421 gcttttacaa tgttcatgct cacgctgcgg ctgtaatcct tcgtagttat gcggataagc
     3481 aaccattaaa tgagtggctc aatgattgga tttttccttt cgaggcaaag atgactgacg
     3541 aagattatta ctggtcaaca ttacttggta tggcggagat ggctaggttt ggcattgtga
     3601 gttgttcgga tatgtatttt ggtggtgagg ctcgcgttaa agctttgctt gaaagtaata
     3661 tgaagtgtaa tacttgcgag gctattgtta gttttgatga tattcatttt tcagagcatc
     3721 ctttatataa agtttctatg gaaatgattg ataaatacaa tggtgccaat aatggaaaga
     3781 ttattactga cttatgcatt catggtgaat atacaacgaa ggagacaatt tgccgtgaaa
     3841 ttgggcagat ggcaaaggat aagaatttaa ttattcacag t
//
LOCUS       DBNCDC010000099         3821 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_16842_length_3821_cov_4.879448, whole genome shotgun sequence.
ACCESSION   DBNCDC010000099 DBNCDC010000000
VERSION     DBNCDC010000099.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16842_length_3821_cov_4.879448"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            158..406
                     /locus_tag="AC04VQ_06625"
     CDS             158..406
                     /locus_tag="AC04VQ_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553778.1"
                     /translation="MKESVVATMGVLFAGAQEIFITFSPLIAISFLTFCLLYTPCIAA
                     IAAARRELGWKWALFIIFFQCAIAWMAAFIVFQIGSLI"
     gene            407..718
                     /locus_tag="AC04VQ_06630"
     CDS             407..718
                     /locus_tag="AC04VQ_06630"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIO9553779.1"
                     /translation="MKLLIFGTGMIVNDYLDGVSEMPFEETYLCGRTKEKVDDLVQKF
                     NLSKAFYDVDEALQSDAEVVYVGLPNNLHFEYAKKSLEAGKHVIVEKPFMPCLQDAQD
                     I"
     gene            743..1366
                     /locus_tag="AC04VQ_06635"
     CDS             743..1366
                     /locus_tag="AC04VQ_06635"
                     /inference="COORDINATES: protein motif:HMM:NF046806.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIO9553780.1"
                     /translation="MIFEASLVFYFPAYKSLVEDLPSLGELHVADCNFSQYSSRYDRF
                     LKGDIAPALNIDNCGGALMDLNVYNINLMVGLFGEPNEATYKPTIINDIDVSGVAVLD
                     YGKLKASCVASKSTSAPRHITLQGIKGNIYLPSSMSRAYEYRIDYHNGETVIRDFKDE
                     HHRMFHEFCEFERCIKEDDFAACESYMNRCFITSKTLDSCRKDAGIT"
     gene            1424..>2280
                     /locus_tag="AC04VQ_06640"
     CDS             1424..>2280
                     /locus_tag="AC04VQ_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HIO9553781.1"
                     /translation="MAKSNTAEMILEEVGGKENVVMLTHCATRLRFSLKDATIIDDKK
                     VDKIPGVLGVVPQGKESYQIVIGGGVAEVYEDINSLLEKKGGAKKSSAKPKEGLSDAD
                     VKAAEREKIKGKSKIADKFFEFLSDSFRPIIGVLLGASLIIAFLNLLISFGFIKDASD
                     TTSTLFLNAIAQSVFYFLPILIAYNACKKLKVDPWVGAVCMLALFTPQFLALLTPDVY
                     ASVFSADGPGLEIAKKSLYTIENPLLGKDGTTFMTTVFGLPLVLNNYKANVFVPLLMS
                     GCLALLYKG"
     assembly_gap    2281..2380
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2381..2574
                     /locus_tag="AC04VQ_06645"
     CDS             <2381..2574
                     /locus_tag="AC04VQ_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553782.1"
                     /translation="VFVPLLMSGCLALLYKGLRKIIPQSVQLVFIPFISMIVIVPVTA
                     FVLGPVGLAGGSLLGMGLS"
     gene            2764..3714
                     /locus_tag="AC04VQ_06650"
     CDS             2764..3714
                     /locus_tag="AC04VQ_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HIO9553783.1"
                     /translation="MVHALRERDTQMSGVAGGALAAGFLGGVSEPSLYGIHLRYKKVY
                     QRLLAGCGAGGITIAVLGFLFPAAAAPGVTTTTFAFTSLLTIPVFDQMWVYTISIAVA
                     FAVSAILVIMFDYRTPQQKAQLRALNAAERLGIVNIDTIFAISEEDQQKMVAKASGDE
                     SSFDDEVFAPVAGTTVNLKNCGDRAFAMKALGEGLAIQPKAGKTKIVAPVAGEITSVS
                     GAKHAYTIMNKNNVGVLVHVGIDTVKLNGKGFNAKVKDGDVVKKGDVLAEVDLEKVEA
                     EGYQTPVIITIVNTTDMKEINVELKDKNVKAGDCIIKIKG"
BASE COUNT         1056 a          623 c          805 g         1237 t
ORIGIN      
        1 aatttgattt ttgcaaagtt ttgattttgg tctaaattac attaccgaaa ccaacgaaag
       61 cattttggct ggtgttgctg gctttatttc gccgattttt gcaccaattg gccttggtga
      121 ttgaagacta gtaacgtcgc taataagtgg gcttttaatg aaagagagcg ttgttgcaac
      181 aatgggagtt ttgtttgctg gcgcacaaga aatcttcatc acattctctc cgcttattgc
      241 aatttctttc ttgacttttt gcttgcttta tactccttgc attgctgcga ttgctgccgc
      301 aagacgtgag cttggatgga agtgggcgtt gtttataatt ttcttccaat gtgctattgc
      361 gtggatggcc gcgtttatcg tatttcaaat agggagctta atttagatga agttgctaat
      421 ctttggcaca ggcatgattg ttaacgatta cttagatggt gtaagcgaga tgccttttga
      481 agaaacttat ttatgtggca gaactaaaga aaaggtcgat gacctcgttc aaaaatttaa
      541 tctttccaaa gctttctacg atgttgacga agctctgcaa tcagacgccg aagttgttta
      601 cgttggtctt ccaaataatt tacattttga atatgctaaa aaatctctcg aagcagggaa
      661 gcatgttatt gttgaaaagc ctttcatgcc ttgcttgcaa gatgctcaag atatttgaga
      721 gatagcaaaa gaaaacaaca agatgatatt tgaggcttcg cttgtatttt attttcctgc
      781 ctacaaaagt ttggtcgaag atcttccaag tttaggagag cttcatgttg cagattgtaa
      841 cttctcgcaa tattcttcaa gatatgatag gtttttaaaa ggtgatatag caccagctct
      901 taatatagat aactgcggtg gtgctttaat ggatttaaat gtttacaata ttaatttaat
      961 ggttgggctt tttggagagc caaatgaagc aacttataaa ccaacaatca ttaacgacat
     1021 tgacgttagc ggcgttgccg ttcttgatta tggaaaacta aaagcaagtt gtgtggcttc
     1081 gaagtcaact tcagcgcctc gccacattac gcttcaagga ataaaaggca acatatatct
     1141 gccaagctct atgagtcgag catatgaata tcgaattgat tatcacaacg gcgagactgt
     1201 tatccgtgat tttaaggacg agcatcatag aatgttccac gaattttgtg agtttgaacg
     1261 ttgcattaaa gaagacgact ttgctgcttg tgaaagctat atgaacaggt gttttatcac
     1321 ctctaaaact ttagattctt gcagaaaaga tgcaggcatt acctgacgct aaaatcattt
     1381 aaataatgtt ataataggga tgctattttc caaaggaggc cccatggcaa aatctaatac
     1441 tgccgagatg attcttgaag aagttggcgg caaagaaaac gttgtaatgc ttacgcattg
     1501 tgcaacgcga cttcgttttt ctcttaaaga tgcaacaatt attgacgata aaaaagttga
     1561 taagatacct ggtgttttag gagtcgttcc tcaaggaaaa gaatcttatc aaattgtaat
     1621 cggcggcggc gttgccgaag tttatgaaga cattaactct cttttggaga aaaagggtgg
     1681 tgctaaaaaa tcaagtgcga agcctaaaga aggtttatcg gatgccgatg tcaaggcagc
     1741 cgaacgtgaa aagattaaag gcaaaagcaa gatcgcggat aaattctttg agtttttgtc
     1801 agattcattt aggccaatta ttggtgtttt gttaggcgct tcgctaatta ttgcattctt
     1861 aaatttgttg atcagctttg gttttattaa agacgcttct gacacaactt cgacattatt
     1921 tttaaatgcc attgctcaga gcgtatttta tttcttgcca attttaattg cttataacgc
     1981 ttgtaagaaa ttgaaggttg atccatgggt tggggcggtt tgcatgcttg ccttgtttac
     2041 tccacaattc ttggctttgc ttacgccaga tgtttatgca agtgtatttt cagcagatgg
     2101 accaggtctt gaaatagcca aaaagtcctt gtatacaatt gaaaacccgc ttcttggaaa
     2161 agatggaaca acgtttatga caacagtatt tggattacct cttgttctaa ataactataa
     2221 ggctaacgtt tttgttcctc tgctaatgag tggatgtttg gctttgttgt ataaaggatt
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn acgtttttgt tcctctgcta
     2401 atgagtggat gtttggcttt gttgtataaa ggattacgta agattattcc tcaatctgtc
     2461 caacttgttt ttataccgtt tatttcaatg attgttattg tccctgttac cgcttttgtt
     2521 cttggacctg ttggtttggc tggtggtagc ttacttggaa tgggactttc atgactaaat
     2581 tcaaacgctc cgtttatttt tgctattgca attcctctgc tgtatccatt cttggtacct
     2641 ctcggattgc actggccgct aaatgctttg atgctcttaa atattgagca gctcggatac
     2701 gattttattc aaggaccaat gggtgtttga aacttcgcat gttttggatg tacttttggt
     2761 gttttggttc atgctcttcg tgaacgcgat actcaaatga gcggcgttgc aggtggtgct
     2821 ttggcagcag gtttcttggg cggcgtttct gagccttcac tatacggtat tcatttgcgt
     2881 tataaaaagg tttatcaaag attactcgca ggatgcggtg ctggtggtat aacaatagct
     2941 gttttaggct tcttgttccc agctgcagct gcaccaggcg taacaactac aacatttgca
     3001 tttacttcgt tgcttacaat tcctgtattt gatcaaatgt gggtatatac tatttcaatt
     3061 gctgttgcct ttgctgtttc ggcaattttg gtaattatgt ttgactatag aactcctcaa
     3121 caaaaagctc aacttcgtgc cttgaatgcg gctgagagac ttggaatagt taatattgat
     3181 acaatttttg cgatttctga agaagaccaa caaaagatgg ttgcaaaagc aagcggggat
     3241 gagtcttcgt ttgatgacga agttttcgct cctgttgcag gtacaacggt caatcttaaa
     3301 aattgcggtg accgagcctt cgctatgaaa gctcttggtg aaggacttgc aattcagcct
     3361 aaagccggaa agactaagat tgttgctcca gttgctggcg aaattacaag tgtttctggc
     3421 gcaaaacacg cttatacgat tatgaacaaa aacaatgttg gagtacttgt tcacgttgga
     3481 attgatactg ttaaacttaa tgggaaaggc tttaatgcca aagttaaaga tggtgatgtt
     3541 gttaagaaag gtgatgtttt agctgaagtt gaccttgaaa aagtcgaggc cgaaggctat
     3601 caaacaccag ttattatcac tatcgttaat actactgaca tgaaagaaat taacgtagag
     3661 cttaaagata aaaacgtcaa agccggcgat tgtataataa aaattaaagg ttagaaaaaa
     3721 aattattttt tcatccaaaa tgcttaattt tattgtataa tacccgaact ttaagattgt
     3781 aaggattacg agagtatttt ggaggaaaaa tggtaaagat t
//
LOCUS       DBNCDC010000100         3768 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_17250_length_3768_cov_7.039321, whole genome shotgun sequence.
ACCESSION   DBNCDC010000100 DBNCDC010000000
VERSION     DBNCDC010000100.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17250_length_3768_cov_7.039321"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="AC04VQ_06655"
     CDS             complement(<1..407)
                     /locus_tag="AC04VQ_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HIO9553784.1"
                     /translation="MKFMTTAEIRSKFLKFFEDKGSKVMPSSSLIPDDPSLLLTSAGM
                     VQFKPYFLQQKKLPKEFIGTATVQKCVRTNDIDIIGTTGRHLTFFEMLGNFSFGQYFK
                     KEMCQWALEFSLDVLGLPFEKLYFSVYKDDDEC"
     gene            complement(423..>1127)
                     /locus_tag="AC04VQ_06660"
     CDS             complement(423..>1127)
                     /locus_tag="AC04VQ_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIO9553785.1"
                     /translation="GRETTKLIPKRFKEDSKMWHVSCTHVMAGFIRGTFLQCLIIGVA
                     SGLAFWIMGMPNSGAFGCIVGVLNIIPIFGPILASIIAFVGGIFNSVWIAILALIVVS
                     AFQGLVYTFISPKIMSSSCSIHPVLTLLCLTAGGAIGGAMSGVIGSLVGMLLAIPFVA
                     LFKSLFVYYYEKNTGKQLVAEDGFLFQGVPYSTKKVIPLYDALEIKPGEEYKIEGRDA
                     KPTKTVKKTTKKAAKK"
     assembly_gap    1128..1227
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1382..1717)
                     /locus_tag="AC04VQ_06665"
     CDS             complement(1382..1717)
                     /locus_tag="AC04VQ_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553786.1"
                     /translation="MRVDQIMENVNQISENAAKATNSVGNLVNAPADLLNKASNKIVG
                     LFGGGKKSEYIGQLEEGDNIDQIVNQSRSQGRKFGRKVVKNDPAASQTATIPQNVNNA
                     SSNPYVSFN"
     gene            complement(1997..2081)
                     /locus_tag="AC04VQ_06670"
     tRNA            complement(1997..2081)
                     /locus_tag="AC04VQ_06670"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2045..2047),aa:Leu,seq:tag)
     gene            complement(2152..2228)
                     /locus_tag="AC04VQ_06675"
     tRNA            complement(2152..2228)
                     /locus_tag="AC04VQ_06675"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2192..2194),aa:His,seq:gtg)
     gene            complement(2284..3552)
                     /gene="glyA"
                     /locus_tag="AC04VQ_06680"
     CDS             complement(2284..3552)
                     /gene="glyA"
                     /locus_tag="AC04VQ_06680"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012799265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     L-serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIO9553787.1"
                     /translation="MEQKINFDETLIALKSNDPEIYELTNKELTRQKESVELIASENF
                     TYPEVMEVAGSILTNKYAEGYPGARYYGGCEVVDEIENVAIDRACKLFSCNFANVQPH
                     SGSSANFAVYNALCNPGDTIMGMTLDNGGHLTHGAPVSFSGKYYNSVSYTLEPETEML
                     NYDKIMEQAKEVKPKLIVTGYSTYPRQIDFAKFREIADEVGAYLMVDIAHIAGLIAGG
                     AHPSPLPYADVVTGTTHKTLRGPRAGMILCNDEEIAKKVDKGVFPGCQGGPLMHIIAA
                     KAVCFNKAMTDEFKTYAHNVVKNTSAMGEAIVEGGLRLVTGGTENHICLIDCQAAKAS
                     GAEVEAACAKANITFNKNGIPFDKLPPTVCSGIRIGAAPVTARGFNDEECRSIASEIA
                     KVAFNINDEAVIAHASEVAKELLAKHPLYE"
     gene            3693..3768
                     /locus_tag="AC04VQ_06685"
     tRNA            3693..3768
                     /locus_tag="AC04VQ_06685"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3726..3728,aa:Lys,seq:ctt)
BASE COUNT         1151 a          838 c          643 g         1036 t
ORIGIN      
        1 atgcattcat catcgtcttt ataaacgcta aaataaagct tttcaaacgg aagccccaaa
       61 acatctaacg aaaactcaag cgcccactgg cacatttcct ttttgaaata ctggccaaaa
      121 ctaaaattac caagcatctc aaaaaatgtc aaatgcctgc ctgtggtgcc aataatgtca
      181 atgtcgtttg tgcgaacaca cttttgaaca gttgcagtcc cgataaattc cttcggcaat
      241 ttcttttgtt gcaagaagta cggcttaaac tgaaccatac cagcacttgt caaaagtagc
      301 gatgggtcat caggaatcag cgaagacgaa ggcatcacct tcgaaccttt atcttcgaaa
      361 aactttaaaa acttagaacg tatttcagca gtggtcataa atttcatggc aagcctactg
      421 gtttactttt ttgctgcttt tttagttgtc ttcttaacag ttttagttgg tttggcatct
      481 ctgccttcaa ttttatattc ttctccaggc ttgatttcca aagcatcata caaaggtata
      541 acttttttag tgctataagg aacaccctgg aacaagaatc catcttcggc aacaagttgc
      601 ttgcctgtgt tcttttcata ataataaaca aaaagcgatt taaacaacgc aacaaaagga
      661 atggccaaaa gcataccaac taatgaacca ataactccac tcatagctcc accaatagct
      721 ccacctgctg tcaagcatag caatgttaaa acaggatgaa tgctgcatga cgaagacata
      781 atcttcggcg atatgaaagt gtaaacaagg ccttggaaag cagaaacaac aataagagct
      841 aaaattgcaa tccaaacact gttaaatatt cctcctacaa aagctatgat agaagccaaa
      901 attggaccaa aaattgggat aatatttaaa acaccaacaa tgcatccaaa agcgccagag
      961 tttggcatac ccataatcca aaatgcaaga ccagaagcaa caccaataat taaacattgc
     1021 aaaaatgttc ctctgatgaa tccagccata acatgagtac aagaaacatg ccacattttt
     1081 gaatcttctt taaaacgctt aggaattaat tttgttgtct ctctaccnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnaag attgttgttc aaattaatat agaaacaggt
     1261 gtggaaagaa caccaaaaag aagtgtaagt aggaaaatta ttgcacaaac acctaatata
     1321 gttgcgcaaa ttaatgctct cttaccccat atttttgttt tgtccataaa accctccgat
     1381 attagttaaa tgaaacatat ggattagatg atgcattgtt aacgttttga ggaatggtag
     1441 cagtttgact agcggcaggg tcgttcttta caactttgcg accaaatttt ctaccttgac
     1501 ttcttgattg gttaacaatt tgatcgatgt tgtcgccttc ttcgagttgt ccaatatatt
     1561 cacttttctt gccaccgcca aaaagaccga caattttgtt acttgcctta ttcaacaaat
     1621 ctgctggagc attaactaag tttccaacag aattagtagc ctttgcagca ttttcagaaa
     1681 tctggttaac gttttccatg atttgatcaa cacgcataag ctcaaggtta gcagcatcaa
     1741 tcgaaagaga aaccctctca ataagtgggt caaccctatc aatggcgggc tggatcttcg
     1801 ttgtgatttg ctcaatattt tcaatagtag gtgttagttg agcgtgaact tcgtcgattt
     1861 ttttacgtga tttacgaagc gtaattacaa gctcgactaa aaatcaagca agagcaatac
     1921 caacgcaaac tccaacaaaa attagcactg cgtaaaaaat tcccatattc atgttctttc
     1981 ctcctaaaat ttttcttggt acgggcgaaa ggacttgaac cttcacgagt tgcctcaacg
     2041 gcacctaaaa ccgtcgcgtc tgccaattcc gccacgcccg cacgtttgat gtattatata
     2101 ttaaaacccc agcatttaca aaaaaattta aaaaaactga aaaaattaga ctggggtggg
     2161 tgacgagatt tgaactcgcg atctcaagag ccacaatctt gcgccttagc caactaggcc
     2221 acacccacca ttgcaagcga gcattatact atttatctcg ccaaccgcaa gaacaaataa
     2281 aaattattca tataacggat gcttggcgag aagttctttt gcaacctcag aggcgtgagc
     2341 aatcaccgct tcgtcgttta tattgaatgc aacttttgca atctccgaag cgatactgcg
     2401 acattcttcg tcattaaatc ccctggcagt aacaggtgca gctccaattc gaataccact
     2461 gcaaactgtt ggtggaagtt tatcaaacgg aataccgttc ttgttgaaag tgatattagc
     2521 cttcgcgcat gcagcctcaa cttcggcacc actagctttt gcagcttggc agtcaataag
     2581 acaaatatgg ttttcggtcc cgccagttac aaggcgaagt ccaccttcga cgatcgcctc
     2641 gcccatcgcc gaagtatttt taaccacatt atgagcataa gttttgaatt catcagtcat
     2701 tgccttattg aaacaaacag ccttggccgc aataatatgc ataagcggac caccttggca
     2761 acctgggaaa acacctttgt caaccttctt agcaatttct tcatcattac acaaaatcat
     2821 accggctctt gggcctcgaa gagtcttatg tgttgttcca gttacaacat cagcatatgg
     2881 aagtggcgaa ggatgagctc caccagcaat aagtccagca atatgagcaa tgtcaaccat
     2941 aaggtaagcc cctacttcat cagcaatttc gcggaacttc gcaaaatcaa tctgccgagg
     3001 ataagtcgaa tagccagtga caatcaactt cggcttgacc tctttggcct gctccataat
     3061 cttgtcatag ttaagcattt cagtttcagg ctcaagcgta tagctaaccg aattgtaata
     3121 tttaccgcta aaacttacag gagcgccgtg cgtcaaatgg ccaccattgt ccaaagtcat
     3181 acccataatt gtatcgcccg gattacaaag cgcattataa acagcgaagt tcgcgctact
     3241 tccactatga ggctgaacat tcgcgaaatt gcagctaaac agcttgcatg cacgatcaat
     3301 tgcaacattt tcgatttcgt caacaacttc acagccacca taataccttg ccccaggata
     3361 cccttcagcg tacttattag ttaaaatact tccagcaact tccattactt ctggataagt
     3421 aaaattttcc gaagcaataa gctcgacgct ctctttttga cgcgttagct ccttgtttgt
     3481 taactcatag atttccggat cgttagattt gagggcaatt aaagtctcgt cgaaattaat
     3541 tttttgttcc aatttggcct cccgcttgag atttttttgc gatgagccta ttatacatat
     3601 ttgaaaatta ttgcaccaat aaattacaac taatcataaa taattcttgc aaaattaaaa
     3661 cccttggcta taatatcact tgctcttttc acggaccggt agctcagctg gcagagcagc
     3721 ggactcttaa tccgaaggtc cggggttcga aaccccgccg gtccacca
//
LOCUS       DBNCDC010000101         3678 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_18028_length_3678_cov_5.817002, whole genome shotgun sequence.
ACCESSION   DBNCDC010000101 DBNCDC010000000
VERSION     DBNCDC010000101.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18028_length_3678_cov_5.817002"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..2949)
                     /gene="nifJ"
                     /locus_tag="AC04VQ_06690"
     CDS             complement(<1..2949)
                     /gene="nifJ"
                     /locus_tag="AC04VQ_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015760978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HIO9553788.1"
                     /translation="MAREFLTMDGNNAAAHVSYAFTEVAGIYPITPSSPMADFVDQWS
                     AQGRKNIFGTKVNVVEMQSEAGAAGAVHGSLGAGALTTTYTASQGLLLMIPNMYKIAA
                     EQLPCVFHVSARTVSSHALNIFGDHGDVYACLQTGFAMLCETNPQEVMDLSAVAHLAA
                     IEGKVPFINFFDGFRTSHEIQKIEVWDYKDLAEMTNMEAVQEFRDNCLNPNRPVMRGS
                     HENGDIFFQHREACNEAYNNLPDVVEKYMKMVNDKLGTDYQLFNYYGAKDADRVIIAM
                     GSINDVAEEVIDYLNANGEKVGLVKVRLYRPFRADKLLAAIPETAKKIAVLDRVKEPG
                     ALGEPLYRDVVTAMANAGREAVICGGRYGLGSKDTPPSSVFAVYDELKKDNPKREFTI
                     GIVDDVTNLSLEEDKNCPNTAAEGTIECKFWGLGGDGTVGANKNSIKIIGDHTDKYVQ
                     AYFQYDSKKTGGVTVSHLRFGDNPIKSPYYVNKADFVACHNPSYITKHFPIVRDVKPG
                     GNLLINCQWTLDELEHHLDAESKRLIAKNKINLYLINAIDLAEKVGMGKRTNTVLQSA
                     FFALAEVLPAKEAIEYMKEKATASYSKKGMDIVEANHKAIDAGATAFEKIDVPESWAN
                     ATDNADDVKIEGRTEVVEQVKNIMFPVGRMDGDSLPVSAFKKYATGQFEHGAAAYEKR
                     GVAVMVPEWDSEKCIQCNSCSYVCPHATIRPFALSEDEAANIPSEAQVVDIKAGAGKG
                     TYKFTIAVSPLDCMGCGVCVGACPDKVQALKMVPMESQLDQQKVFDYCVANVKEKEDM
                     AGDTVKASQFKKPLLEFSGSCAGCAETSYARLVTQLFGDQMYISNATGCSSIWGGPAA
                     TSPFTTNEKGHGPAWCNSLFEDNAEHGFGLYLGQQAIREQLINTATEMLDCDKASAAT
                     KEAAKVYLDTKDDTIANAKASEELIKLCEGESECPHAKEILAKKDYLSKKSVWIFGGD
                     GWAYDIGYGGV"
     gene            3248..3586
                     /locus_tag="AC04VQ_06695"
     CDS             3248..3586
                     /locus_tag="AC04VQ_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIO9553789.1"
                     /translation="MKDLANADIKKVEQILRSIGFYQNKAKNSINCAKMILEDFNGSV
                     PDNMEDLQKLPGVGRKTANIVLNVAFDKVEGIAVDTHVFRISHCLKLSSAKTPEKTEP
                     DLLKIFDKKD"
     gene            3611..>3678
                     /locus_tag="AC04VQ_06700"
     CDS             3611..>3678
                     /locus_tag="AC04VQ_06700"
                     /inference="COORDINATES: protein motif:HMM:NF022048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIO9553790.1"
                     /translation="MFGRQTCIARNPKCHACPLAKL"
BASE COUNT         1115 a          799 c          684 g         1080 t
ORIGIN      
        1 aacaccaccg tatccaatat catatgccca accgtcacca ccaaagatcc aaacagattt
       61 cttcgataga tagtctttct ttgctaagat ttctttagca tgtgggcatt cgctttcgcc
      121 ttcgcagagt ttaattaact cttcagaagc tttagcgttt gcaatcgtgt catcttttgt
      181 atcgagataa actttggcag cttctttagt agcagcactt gctttatcgc agtcgagcat
      241 ttcagtggca gtattgatta gctgctcacg aattgcttgc tgtccaagat acaaaccgaa
      301 gccgtgttct gcgttgtctt caaaaagcga attacaccaa gcaggaccat ggcccttttc
      361 attggttgta aacggcgaag tagcagcagg accgccccaa atacttgaac aacctgttgc
      421 gtttgagatg tacatttgat caccaaataa ttgagtaacc aaacgagcat aagaagtctc
      481 ggcacaacca gcacaagaac cactgaactc aagaagaggt ttcttgaatt gtgaagcctt
      541 tactgtatct ccagccatgt cttctttttc tttaacatta gcaacgcagt aatcaaatac
      601 tttttgttgg tctaattgag attccattgg aaccatcttt aatgcttgaa ccttatcagg
      661 acaagcacca acgcaaacac cgcatcccat acaatcaagt gggcttacag caattgtaaa
      721 cttataagtt cccttgcctg ctccagcttt gatatcaaca acttgagctt cgcttgggat
      781 gttggcagct tcatcttcgc taagagcaaa tggacgaatt gttgcatgag gacaaacata
      841 tgaacagctg ttacattgaa tacatttttc gctatcccat tctggaacca taacagcaac
      901 gccacgtttt tcgtaagctg cagcaccatg ttcaaattga ccagtagcat atttcttaaa
      961 tgcagaaact ggaaggctat caccatccat tcttccaact gggaacataa tgtttttaac
     1021 ttgctcaaca acttctgtcc taccttcgat tttaacatcg tcagcattgt cagttgcatt
     1081 tgcccagctt tcaggaacat caattttctc aaatgctgta gcaccagcat caatagcctt
     1141 atggttagcc tcaacaatgt ccataccctt cttggaatat gaagcagttg ccttttcttt
     1201 catgtattca attgcttcct tagcaggcaa aacttcggcc aacgcaaaga atgctgattg
     1261 aagaactgta ttagtacgct ttcccatgcc aaccttttca gccaagtcaa tagcattaat
     1321 taggtaaagg ttgatcttgt tcttcgcaat gagtctctta gattcagcat ctaagtgatg
     1381 ctcaagttcg tcaagagtcc attggcagtt aatcaaaaga tttccgcctg gtttaacatc
     1441 gcgaacaatt gggaaatgct tagtaatata agaaggatta tggcaagcaa caaaatcagc
     1501 cttatttaca taataagggc tcttgattgg attgtcgccg aagcgtaagt gagaaacagt
     1561 tacgccgcca gttttctttg aatcatattg gaagtatgct tgtacatatt tgtctgtatg
     1621 atcaccaata atcttaatcg agttcttgtt agcaccaact gtaccatcac cgccaagacc
     1681 ccagaacttg cactcgattg tgccttcggc agctgtgttt ggacaattct tgtcttcttc
     1741 aagagataag ttagtaacgt catcaacaat accaattgtg aactcacgct taggattgtc
     1801 tttcttaagc tcgtcataaa cagcgaagac acttgaaggt ggtgtatcct ttgaaccaag
     1861 accataacga ccaccgcaaa ttacagcttc gcgaccagcg ttagccatag ccgtaacaac
     1921 atctctgtaa agaggttcgc ccaaagcgcc tggttcttta acgcggtcaa gaacagcaat
     1981 tttctttgca gtctcaggga ttgcagcaag aagtttatct gcacggaaag gtctgtataa
     2041 acgaacctta acaagaccaa ctttttctcc atttgcattt aagtaatcaa taacctcttc
     2101 agcaacatca ttgatagaac ccattgcaat aattacacgg tcagcatctt ttgcaccata
     2161 gtaattaaat aattgataat cggttcccaa cttatcgttg accatcttca tgtatttttc
     2221 aacaacatca ggaaggttgt tgtaagcttc gttgcaagcc tcgcgatgct ggaagaaaat
     2281 gtctccattt tcgtgagaac cacgcataac tggcctattt ggatttaagc agttatcgcg
     2341 gaattcttga acagcttcca tgttggtcat ctcagctaag tctttgtaat cccaaacttc
     2401 aatcttttga atttcatgag atgttctaaa accatcgaag aagttgatga atggaacctt
     2461 accttcgata gctgctaagt gagcaactgc agataagtcc ataacttctt gagggttagt
     2521 ttcgcacaac attgcaaaac cagtttgcaa acatgcataa acatcgccat gatcaccaaa
     2581 aatgtttagc gcatgagatg aaacagtacg tgctgaaaca tgaaatacgc atggaagttg
     2641 ctctgcggca atcttgtaca tatttggaat catcaacaat agaccttgcg aagctgtgta
     2701 agttgtagtt aaagcaccag ctccaaggga tccgtgaaca gcaccagcag caccagcctc
     2761 tgattgcatt tctacaacat taacttttgt gccgaagatg tttttacgtc cctgagcaga
     2821 ccattggtct acaaagtctg ccattggtga ggatggtgta attggataaa taccagcaac
     2881 ttcagtaaaa gcatagctta cgtgagctgc agcattgtta ccatccatag taagaaattc
     2941 tctcgccata attctctcct tctataattt gtaaaacgaa atttagcgca attgcatata
     3001 atatcactta tgttagtttt tacaataaca atttaggtga tttatggctt atgaaaacgt
     3061 taaagataaa cgtgagcgag cttcgaaaat tgcgaagatt ttaaacgaag ttctacctaa
     3121 tccagataca gagcttaatt attgaggcga tccttttcgt cttgttattg cagttgccct
     3181 atcagctcaa acaacagata aatcagtcaa taaggtaacc ccgctccttt gagaaaccta
     3241 ccctactatt aaagatttgg caaacgccga tataaaaaag gttgaacaga tacttcgaag
     3301 cattggtttt tatcaaaaca aggcgaagaa ttcaatcaac tgtgcgaaga tgattcttga
     3361 agattttaat ggctccgttc ctgacaatat ggaagatctt caaaagcttc ccggagttgg
     3421 aagaaaaact gcaaatatag ttttaaatgt ggcatttgac aaggtcgaag gcattgcggt
     3481 agatacccat gtttttagaa tttcgcactg tcttaagttg agcagcgcga agactcctga
     3541 aaaaactgag cctgaccttt tgaaaatatt tgataaaaaa gattgaaaaa acctaaatca
     3601 ccaattggta ttgtttggca gacaaacttg catagcaaga aacccaaagt gtcatgcttg
     3661 tccccttgca aaactttg
//
LOCUS       DBNCDC010000102         3485 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_19835_length_3485_cov_8.146356, whole genome shotgun sequence.
ACCESSION   DBNCDC010000102 DBNCDC010000000
VERSION     DBNCDC010000102.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19835_length_3485_cov_8.146356"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(100..1500)
                     /locus_tag="AC04VQ_06705"
     CDS             complement(100..1500)
                     /locus_tag="AC04VQ_06705"
                     /inference="COORDINATES: protein motif:HMM:NF013362.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIO9553791.1"
                     /translation="MSSLRVENSGKLSKARIASLNAIMDIRKNEAFARDVLNKTLNDH
                     VLSGADRAFAAKLTLGVTQTLGTLDDVIYRCVDSPDDIKSNVMDCLRISTYEIIYLEK
                     EPHAAVDQGVELVKAVEPRARGLANLVLRRVVKLKRDFPFGNPDEDTEAFARIYAIPK
                     WECELIFKSIGENAGRYLISSSNGAPPAYVFVNSIKSSRDEVFEILKAAEAKPSVVEA
                     LCGIKLNGCIRVENSAALNNTEIKQAMMMGKFSISDAASQAVATHAMQAFENPQTMLE
                     VCAGKGTKTLMFQSLANEKWGHQMDLCTVDNVEAKTKILRERAKRYGVKLTRTLTADA
                     SSDTAFKRAIGQAKFDIVFVDTPCSGLGTLRRHPELKWRLTEKKINQMASTSFKILTN
                     AAKHVVEGGVLTYATCTVTKAENEDVVERFLKSPLGQKFKLIPYQVDDKQMPHFRTQT
                     FSGLNDSHFSATFKKV"
     gene            complement(1493..2404)
                     /gene="fmt"
                     /locus_tag="AC04VQ_06710"
     CDS             complement(1493..2404)
                     /gene="fmt"
                     /locus_tag="AC04VQ_06710"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIO9553792.1"
                     /translation="MRIVFMGTPDFAKNILQGLVESAECEVVAVYTREDAVRGRGKNL
                     VPSPVKEFAVENNIPVETPKNFNDEDTVAKLASYKPDFIVVAAYGLILPKPVLDIPKY
                     ECLNVHGSLLPKWRGAAPVQRAILAGDAESGFTIMRIGEGLDDGDMYSRHAFDITDMS
                     TTEVLDEISKRSVPELLKTMHEIESGEAKAEAQDDSLAIFADKIEKRELWYKAGDSDE
                     IWYRKILASTPESPAKCIIAGRIVTITKIECGPRGNRVNATGKSSVGPGEIERVRPDG
                     KNEMSFKDFVNGLQNVDIDNLGFEEIE"
     gene            complement(2522..3025)
                     /gene="def"
                     /locus_tag="AC04VQ_06715"
     CDS             complement(2522..3025)
                     /gene="def"
                     /locus_tag="AC04VQ_06715"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012798130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIO9553793.1"
                     /translation="MLEVVKSPNQILLERAVECDPSDSSLKNLSREMLETMYKNHGIG
                     LAGPQVGELKRIIVIDLGYDPEDKDKPKCPHTLINPEIVERSSQMEDSEEGCLSCPGI
                     SAPVLRHAIVKVKYRDLDGQEQTIEAGGLLSHCLQHEIDHLDGKTLFQTAKPEVRLQL
                     LQDYQLS"
     gene            complement(3025..>3485)
                     /locus_tag="AC04VQ_06720"
     CDS             complement(3025..>3485)
                     /locus_tag="AC04VQ_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553794.1"
                     /translation="TKAAPKSSKLADLEKELLSLIVKYPGETYKFISENFEHKWSSAS
                     HQKLFEFITQTIASNNDIKSQELMMKIHQKNDKTLSKLTNDSKRYDFQNKPDKHVQYL
                     KLLIKAEDISVEISNSKQLFKSNNSDELFKKIIELENALNEMRGKIREMI"
BASE COUNT          889 a          891 c          734 g          971 t
ORIGIN      
        1 aatcctccac tcaacaaatt aaaaattata tcacacaaaa ggtgtatttc atgcctggca
       61 ataattacac cttttggtgc atttcatgcc tggcaataat tacacctttt tgaaggttgc
      121 ggaaaagtgc gagtcgttta ggccgctgaa agtttgcgtg cgaaagtgcg gcatttgctt
      181 gtcatcaact tgataaggaa tgagtttgaa tttttggccg agcggacttt tgagaaagcg
      241 ctcaaccacg tcttcgtttt ccgctttggt tacggtgcaa gttgcatagg tcaaaacgcc
      301 gccttcgacc acgtgttttg cagcgtttgt caaaatctta aagctagtgc ttgccatttg
      361 attgatcttt ttttcggtta aacgccactt aagttccgga tgccgacgaa gcgttccaag
      421 cccactgcaa ggcgtgtcca caaaaacaat gtcgaatttt gcttgaccaa tcgctcgctt
      481 aaaagcagtg tcgctcgatg catcggctgt caatgttctc gtaagtttca cgccataacg
      541 tttggcgcgc tcacggagga tcttcgtctt cgcctcgaca ttatcaacgg tgcacaaatc
      601 catctggtgg ccccacttct cgttcgccag gctttgaaac atcagcgttt tagtgccctt
      661 acctgcgcaa acttcaagca ttgtctgggg attttcgaag gcttgcatag cgtgggttgc
      721 aactgcttgc gaagccgcat cgctaatcga aaacttgccc atcatcatgg cttgcttaat
      781 ttcggtgttg ttcagcgccg cggaattttc aactcgaatg catccgttca acttaattcc
      841 gcacaaagct tccaccacgc taggcttggc ttcggcagct ttcaagattt cgaaaacctc
      901 atcgcggcta ctcttaatag aatttacgaa gacatatgcc ggcggcgcac cattgctcga
      961 agaaattaga tagcgcccgg cgttttcgcc aatgcttttg aaaatcagct cgcattccca
     1021 ttttggaatc gcgtaaatac gagcgaaggc ttcggtgtct tcgtcagggt ttccgaaagg
     1081 aaaatcgcgc ttcagcttaa cgacgcgtcg caaaaccaaa ttcgcaagcc cgcgagcgcg
     1141 aggctctacc gctttgacca gctcaacacc ctggtcaacc gcagcgtgcg gctccttttc
     1201 aagataaata atttcatagg tgctaatgcg aaggcagtcc atcacgttgc ttttgatgtc
     1261 gtccggactg tccacgcacc gataaatcac atcgtcgagc gtgcccaaag tttgagtaac
     1321 gcccaaagtc agcttcgcag cgaaggccct gtccgccccc gaaagcacat gatcattcaa
     1381 agttttattt aaaacgtcgc gcgcgaaagc ctcgttcttg cgaatatcca tgattgcgtt
     1441 cagcgacgca atcctcgcct tcgaaagctt cccagaattt tcaacccgca aactactcaa
     1501 tttcttcaaa ccccaaatta tctatatcca cattttgaag accgtttaca aaatctttaa
     1561 aactcatttc gttcttgcca tccggcctaa ccctctcgat ttccccgggg cccacactcg
     1621 atttgcccgt ggcatttact cgatttcccc ggggcccaca ctcgattttt gtaattgtaa
     1681 caatccgacc tgcaattatg cacttcgcag gactctctgg cgtagatgcc aaaatcttgc
     1741 gataccaaat ttcgtcagaa tcgccagctt tataccaaag ttctcgtttc tcaattttat
     1801 cagcgaagat cgccagcgaa tcgtcctgag cttcagcctt tgcttcgcca ctttcaattt
     1861 cgtgcattgt tttaagcaac tccggaacag aacgcttcga aatttcatcc aaaacttcag
     1921 tcgtgctcat gtcagtaatg tcaaaagcat gacgagaata catgtcgcca tcgtctagcc
     1981 cttcgccgat acgcataatc gtaaaaccgg actcagcatc accagcaaga attgcacgct
     2041 gaacaggagc agcacctcgc cacttcggaa gcaaagatcc gtgaacattt aaacattcat
     2101 atttgggaat gtccaaaact ggcttcggaa gaattaaacc atacgccgca accacaataa
     2161 aatcaggctt gtaacttgca agtttggcaa ctgtgtcttc gtcattaaaa tttttaggag
     2221 tttcaaccgg gatgttattt tcgaccgcga attccttgac agggctcggc accaaatttt
     2281 tacctcgacc gcgaaccgcg tcttccctcg tgtaaacagc aacaacctca cattccgcgg
     2341 actcaacaag tccctgcaaa atgttcttcg cgaaatcagg tgtccccata aacacaattc
     2401 tcataacgcg cctattttag cacaaaaata aaaatcgagt aaatgccacg ggtaaatcga
     2461 gttctggccc cggggaaatc gagtaaatgc cacgggtaaa tcgagtactg gccccgggga
     2521 attaagagag ttggtagtct tggaggagtt ggaggcggac ttcgggtttt gcggtttgga
     2581 agagagtttt gccatcgagg tgatcaatct catgttggag acaatgcgaa agcaggccgc
     2641 cagcttcgat agtttgctct tgcccgtcga gatcccgata cttaacctta acaattgcgt
     2701 gacgaagcac cggcgccgag attcccggac acgaaaggca accttcctcg gaatcttcca
     2761 tctgggacga acgctcaaca atttcagggt tgattaaagt gtgcgggcac ttcggcttgt
     2821 ctttgtcttc tgggtcataa ccaaggtcaa tcacaatgat gcgttttagc tcaccaacct
     2881 gcggaccagc aagcccgatc ccgtgatttt tatacatagt ttcaagcatt tcgcggctca
     2941 aatttttaag cgacgaatcg ctagggtcgc actcgaccgc gcgctcaagc aaaatttgat
     3001 tcggcgattt cacaacctcg agcattaaat catctcccga attttcccgc gcatttcgtt
     3061 tagcgcattt tcaagctcga taattttttt gaacagctca tcgctattgt tagatttaaa
     3121 aagttgcttg ctgtttgaaa tctcaacaga aatatcctcg gccttaatca gcaacttgag
     3181 gtattgcaca tgcttgtcag gtttattttg aaagtcgtag cgcttcgaat cattcgtaag
     3241 cttgctcaac gtcttgtcat ttttctgatg aattttcatc atgagctctt gcgatttaat
     3301 atcgttgttg ctcgcaatcg tctgcgtgat aaattcaaaa agtttttgat gcgaggcgct
     3361 tgaccacttg tgttcaaaat tttcgctgat gaatttatac gtctcacctg ggtatttaac
     3421 aatcaagctt aagagttcct tctcaagatc cgcaagcttt gaacttttcg gtgccgcctt
     3481 tgtag
//
LOCUS       DBNCDC010000103         3435 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_20364_length_3435_cov_6.978402, whole genome shotgun sequence.
ACCESSION   DBNCDC010000103 DBNCDC010000000
VERSION     DBNCDC010000103.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20364_length_3435_cov_6.978402"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(234..491)
                     /locus_tag="AC04VQ_06725"
     CDS             complement(234..491)
                     /locus_tag="AC04VQ_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HIO9553795.1"
                     /translation="MQKVTIYTDGACRGNPGNGGYGCVLLYTDEAGQTHRQEFSEGFK
                     DTTNNRMELMAVIVAVEALKYPCEVEIFSDSKYVVDAFNQN"
     gene            complement(984..1226)
                     /locus_tag="AC04VQ_06730"
                     /pseudo
     CDS             complement(984..1226)
                     /locus_tag="AC04VQ_06730"
                     /inference="COORDINATES: protein motif:HMM:NF015013.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
     gene            complement(1245..1391)
                     /locus_tag="AC04VQ_06735"
     CDS             complement(1245..1391)
                     /locus_tag="AC04VQ_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553796.1"
                     /translation="MKDPALLGAIKIAVPTFIYVLGMLVAFTCRNAFSLEISDSGPAT
                     ILYA"
     gene            complement(1407..2036)
                     /locus_tag="AC04VQ_06740"
     CDS             complement(1407..2036)
                     /locus_tag="AC04VQ_06740"
                     /inference="COORDINATES: protein motif:HMM:NF015013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HIO9553797.1"
                     /translation="MSGATLICRITGLIRTWAMAFALGNTVITSAYQVANNMPNIIFD
                     LVAGGLLGAAFIPIFMLEKEQHGSKGGNRFACNMLNLTIILMAIFSILMTIFAPQVIA
                     TQTFTVGQQAEVSEIAVIFFRIFAIQMLFYGIGGVLNAILNGERIYFLPAVAPAINNI
                     VVIASFGAYALLYQSDQMLAIIILGIGTTLGVAVQAIVQIPALLKTGFK"
     gene            2175..3230
                     /locus_tag="AC04VQ_06745"
     CDS             2175..3230
                     /locus_tag="AC04VQ_06745"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIO9553798.1"
                     /translation="MKAIKTKYIAIIFASVLLAVVSVFAISSEFAYAWNTTQWRPSVE
                     IKKSIDSSYAKIAEYNDEIEESKNFIEAYESNAVIYEEDVRVLQAKFDDMQKYIDEQK
                     PELEEITKSSYAAFFAKGLLDEMVGNRFITNGLFRWNFVTFFACSEDDEIMNFIEDYY
                     NTKKELDSAISENEEATTKYNQSLSNIKNLPIEINSLNQNIANLNNMLPEILEIEKVF
                     GAAGESRIEGSNFFCHPCPDSQISSPFGESRDGYTHHGCDFSAPLGSPIYCAADGVVF
                     ETGQSDSMGNYIKIQHSDGLVSVYMHCSHVFVPNGMQVSRGDNIALVGSTGDSEAPHL
                     HFQVELNGTPVNGLDYM"
BASE COUNT         1108 a          625 c          641 g         1061 t
ORIGIN      
        1 ggcaataatt acaccttttg gtgcatttca tgccaggcaa taattacacc tttttagtgg
       61 atggaatcgg cggcttgtgt ggcgagttgg tcgcagcgtt cgttttcttc attgcctgcg
      121 tgaccttcga ctcaagtgaa cttatatttg tgttgctcaa gagcagctaa aaggcgcttt
      181 caaagctcga tgtttttaac cttcttccaa ttgcgttttt ctcaaccttc aattcaattt
      241 tgattaaacg cgtccactac gtatttagaa tcggaaaaga tctcaacttc gcaagggtac
      301 ttcaacgctt caacagcaac aattacagcc ataagttcca ttctgttatt ggtggtgtct
      361 ttaaagcctt cgctgaattc ttggcgatgc gtttgtccgg cttcgtcggt atatagaaga
      421 acacaaccat aacctccatt gccaggattt cccctgcatg cgccatctgt gtatattgtt
      481 actttttgca tttaattcct cttgaactta ttttttattg catcgaacaa ttgaaggatt
      541 tctttaactc taaatattca acagaaaaga ccaacaacta aaaggctaat tgtgccgcaa
      601 acaacaagct ggaaaactcc atttagtata cttgttgaac ccaggtcgat gtagttcatc
      661 aaaacaatac caacaaaaca tccaatcgac gaagctaaac atactcgaat cgcgcttcaa
      721 ataattttgc caacatcgaa gcttccaatc ttcgatttca aaattattag aagggctata
      781 caaattcctg cgtaaaaaat aaagtccgca attggaattc caagcaagcc aaatgaatca
      841 aattgtgtgc aaaacaacca gtaaaaaaag cactggccaa taacactaat tatgctgaca
      901 actgcaaata ttgtaaactt tcgaattgat gcatatacgt tatacaaata cataacaaca
      961 gaataaaatg gcaagcttac aactcaaact tgaaggacaa gcgctactgt ttgcacatcg
     1021 cctgcattaa atgctccagc tctaaataat gacattagag gaagggcgaa ggttcccatc
     1081 aacattgcca atggaataat tgcaattagg gtaatagata aaccggtgtt gacttggctt
     1141 cgtaatttta taatgttgcc tttagcgctt gaatcgctca tttctgtaaa catagttcgt
     1201 gaaagtgaaa cagcaattac tccgtaagga agttgatatc atgttcacgc gtatagaatt
     1261 gttgctggtc cactatctga aatttcaagc gagaaagcgt tgcgacatgt gaaagcaaca
     1321 agcattccaa gtacgtaaat aaatgttgga actgcaattt ttatagcgcc aagcaaggct
     1381 ggatctttca aatcaacaat tggctgtcat ttaaatccag tttttagaag ggcagggatt
     1441 tgaacaattg cttggacggc aacacctaat gtagtgccaa ttccaagaat aattattgca
     1501 agcatttgat cgctttgata aagcagcgca taagctccaa acgaagctat aacaacaatg
     1561 ttatttatcg caggagcaac agcaggtagg aaataaattc tctcgccgtt aaggattgca
     1621 tttaggacac caccaattcc gtagaaaagc atttgaattg caaaaatacg gaaaaatatt
     1681 actgcaattt cgcttacttc ggcttgttgg cctactgtaa aagtttgtgt agcaataact
     1741 tgaggagcga aaattgtcat taatatcgaa aagatcgcca tcagaataat tgttagattt
     1801 aacatgttgc aggcgaagcg gtttccacct ttggaaccat gctgttcttt ttcaagcata
     1861 aaaattggaa tgaaagcagc tccaagcaaa ccacctgcaa caaggtcaaa aattatgtta
     1921 ggcatattat tcgcaacttg ataggccgag gtaatgacag tattgcccaa agcaaaagcc
     1981 attgcccacg tgcgaattaa acctgtgatt ctgcaaatta gagtagcgcc agacatggcg
     2041 acagtacttt ttgcgacaga tgaagcggta ctactttttt tgttcgtttt tacttcattc
     2101 atatgaacaa ttttagcgta atttaaggct cttttacctt atttttaagt ataatgtacc
     2161 tgctataata agttatgaaa gctataaaaa cgaaatatat tgcgattatt tttgcatctg
     2221 tacttcttgc tgttgtcagc gtttttgcaa tatcaagtga atttgcttat gcttggaaca
     2281 caacgcaatg gcgtccaagt gttgaaatta agaaaagcat agattcatct tatgcaaaaa
     2341 ttgctgaata caatgatgaa atagaagaaa gtaaaaactt tattgaagct tatgaaagta
     2401 atgctgtaat ttacgaagaa gatgttcgtg ttcttcaagc aaaatttgat gatatgcaaa
     2461 aatatataga tgaacaaaag ccggaacttg aagagataac aaaaagtagt tatgcggctt
     2521 tctttgctaa aggattgctt gatgagatgg ttggaaatcg tttcattacc aatgggcttt
     2581 ttagatggaa tttcgtaaca tttttcgctt gtagcgaaga cgatgaaatc atgaacttta
     2641 ttgaggatta ttacaataca aaaaaagagc ttgatagtgc tattagcgaa aacgaagaag
     2701 caacaactaa gtataatcaa tcattgtcaa atattaaaaa tttgccaatt gaaattaatt
     2761 ctttgaatca aaacatcgct aatttaaata atatgttgcc agagatttta gaaattgaaa
     2821 aagtttttgg tgctgcaggt gaaagcagaa ttgaaggatc gaattttttc tgtcatccat
     2881 gtcctgatag tcaaattagt tctccatttg gtgagagcag ggatggttat acgcatcacg
     2941 gctgtgattt ttctgcgcct ctaggttcgc ctatttattg tgctgctgac ggtgttgttt
     3001 ttgaaactgg ccaaagtgat tcgatgggaa attatattaa gattcaacat agcgatggat
     3061 tagtttcagt ttatatgcat tgcagtcacg tttttgtgcc aaatgggatg caagtatcta
     3121 gaggcgacaa cattgcttta gttggaagca caggtgatag cgaagcccca catcttcact
     3181 ttcaagttga acttaatggc acaccagtta acggtttaga ctacatgtag aattaatctt
     3241 cgttttcctc atcatcagtt tttggaattg tatcgtcata agatcaatct tgaccactaa
     3301 ttcatttatc aagttcttcc tgacttatca ttacgaaggc ttgtgaattt tgtttttcaa
     3361 atatatctcg aactgtttct atgctgtaat aaacatctcc attaatcgaa tcagcaacat
     3421 atacaattcc ttctt
//
LOCUS       DBNCDC010000104         3427 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_20439_length_3427_cov_7.998221, whole genome shotgun sequence.
ACCESSION   DBNCDC010000104 DBNCDC010000000
VERSION     DBNCDC010000104.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20439_length_3427_cov_7.998221"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /gene="groEL"
                     /locus_tag="AC04VQ_06750"
     CDS             <1..1296
                     /gene="groEL"
                     /locus_tag="AC04VQ_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIO9553799.1"
                     /translation="RGIQKATDAVVDKIKANSKSVSTKDQIASVGTISAGDAEIGGKI
                     AEAMEAVGKDGAISVEENQTSFGIDMEIVEGMQYDQGYLSPYMATDTEKMEAVLNNPY
                     ILITDQKISNIQEMVKLLEEVMHAGRPLVLIAEDVEGEALATLLVNKLRGTLNCVAIK
                     APGFGDRRKRILEDIAIVTGANVIDKDLGINLADANVEMLGSAKTVRVDKDNFLIVDG
                     AGSKSAIKDRCEQIKAEIERSDSDFDREKLTERLSKLSGGVAVLQVGAATESELKEKK
                     SRIEDALQATRAAVEEGIVAGGGVALLNAVPALDKVKGETADENIGVEIVRKALEAPL
                     RAIASNAGFEGSVVVEKVKGLKVGEGLNCANGEYGDMIKMGVNDPVKVTRTALQSAVS
                     VASLILITEATVNDIPKDPDPAAAAAAMAGAGGGMPGMM"
     gene            1510..2034
                     /locus_tag="AC04VQ_06755"
     CDS             1510..2034
                     /locus_tag="AC04VQ_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012804.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIO9553800.1"
                     /translation="MLEKLKEEVLIANKRLVESGLVVLTWGNASAFDPESKLLVIKPS
                     GVDYDKMKASDMVVVDLNNEVVEGDLRPSSDTPTHVEIYKAFADAGVRGVVHTHSTEA
                     TAWAQAGRSIPWYGTTHADQCHEDVICTRPLTAEEVEQDYEGNTGRVIVDAYKGINPL
                     EKPVVLVAGHAPFT"
     gene            2074..2211
                     /locus_tag="AC04VQ_06760"
     CDS             2074..2211
                     /locus_tag="AC04VQ_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553801.1"
                     /translation="MEEFAKMARLTEQIASSKPAQKLPDYVGEKHYQRKHGKNAYYGQ
                     N"
     gene            2617..>3427
                     /locus_tag="AC04VQ_06765"
     CDS             2617..>3427
                     /locus_tag="AC04VQ_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIO9553802.1"
                     /translation="MPSLTNQVSGKVTDVNGAGIPNVTLEFYNPFSVKSPFVDIAAKT
                     DAEGNYSLESPIVSSDYYSGVSIGSDYYVVAQTGECEKGYLSKTLDLPEVQESANVLN
                     FQLDRIVNLKLYDYDQTENGWAEWYHELYINGELQSEGSYLGDSNFQLPLNAKFTFKD
                     NCKLELAYTLGEDEYLDTFVPAFRNAIKWEGQDFNENLIGDYWMFWINEKSEGTPPIE
                     CRDGYSYQCDNSTNYLYLDVNFKAKNVEPAVVTPAQTGDENGMLIAILIAVV"
BASE COUNT         1022 a          652 c          791 g          962 t
ORIGIN      
        1 cgaggcattc aaaaggcaac cgatgctgtt gttgacaaga ttaaggcaaa ctctaagagc
       61 gtttcaacca aggatcaaat tgccagcgta ggtacaattt ctgctggcga tgctgagatt
      121 ggcggcaaga ttgctgaagc catggaagcc gttggtaagg atggcgctat cagcgtcgag
      181 gaaaaccaga cttcgtttgg catcgatatg gaaattgtcg aaggtatgca atatgaccag
      241 ggttatttaa gcccttacat ggcaaccgac acagaaaaaa tggaggcggt tcttaacaac
      301 ccttacattt taatcaccga ccaaaaaatt tctaacattc aagaaatggt taaacttctt
      361 gaggaagtta tgcatgctgg tcgtccactt gttttgattg ccgaagatgt cgaaggcgaa
      421 gctcttgcaa ctttgcttgt taacaaactt cgcggcacct tgaattgtgt tgcaattaag
      481 gctcctggat ttggtgaccg ccgcaagaga attcttgaag atattgctat tgttaccggc
      541 gcaaacgtaa ttgataagga ccttggaatt aacttggccg atgccaatgt tgaaatgctt
      601 ggttccgcaa aaactgttcg tgttgacaaa gacaacttct tgatcgtgga tggtgctggt
      661 tctaagagtg ctattaaaga ccgttgcgag caaatcaaag ctgaaattga acgcagcgat
      721 agcgatttcg atcgcgaaaa attgaccgag cgcctttcta aactttctgg cggagttgct
      781 gttcttcaag ttggtgctgc tacagaaagc gaactcaagg aaaagaaatc tcgcatcgaa
      841 gacgcgcttc aagctactcg tgcagctgtc gaagaaggca ttgttgctgg tggtggcgtt
      901 gctcttctaa atgctgttcc tgctcttgac aaagttaagg gtgaaactgc ggacgagaac
      961 atcggagttg aaattgttcg taaagctctc gaggctccac ttcgcgctat tgcatctaac
     1021 gctggtttcg aaggcagcgt tgttgttgaa aaagttaagg gcctcaaggt tggcgaaggt
     1081 cttaactgcg ccaacggcga atatggcgac atgattaaga tgggcgttaa cgacccagta
     1141 aaagttacaa gaactgctct tcaaagtgcg gtttctgttg cttcgctaat tctgatcacc
     1201 gaagctaccg taaacgacat tccaaaagat ccagatcctg ctgctgcagc tgctgcaatg
     1261 gctggtgcag gcggcggaat gccaggcatg atgtaaaata caaatttacc taacggtaaa
     1321 tttattcagt aaaaggtgcg caattatatt tgtcgcacct ttttgcttgt tttggggctc
     1381 gcgtattcac gatacgcgtc gccccaaagg cacaaaaatc tgcttccaaa tataactgct
     1441 cccaacaatc cttaaattat attcgaactt tttgcatgtg ttataattga cataacggaa
     1501 ggagaattta tgctagagaa acttaaagaa gaagttttaa ttgcaaataa aagattagtt
     1561 gagtctggat tagttgtttt aacctggggg aatgcttcgg cgtttgatcc tgagtcaaag
     1621 cttttggtaa tcaaaccttc gggtgttgat tatgacaaaa tgaaggcaag cgacatggtt
     1681 gttgttgatt taaacaacga agttgtcgaa ggtgatcttc gtccttcaag tgacacacca
     1741 actcatgttg aaatttataa agcgttcgcc gatgcaggag tgcgtggcgt tgttcacact
     1801 cattctaccg aagccacggc gtgggctcag gctggacgtt caattccttg gtacggcaca
     1861 acgcacgccg atcaatgcca tgaagacgtc atttgcacaa ggcctttgac cgccgaagaa
     1921 gtcgagcaag attacgaagg caacacgggc cgcgtaattg tcgatgctta taagggcatc
     1981 aatccgcttg agaagccagt cgttttagtt gctggacatg caccttttac atgaggcaag
     2041 aatgctaatg ccgcagttga taatgcaatt gctttggaag aatttgcaaa gatggctcgt
     2101 ctcaccgagc aaattgcttc gagcaagcct gcgcaaaaac ttccagatta tgttggcgaa
     2161 aaacactatc aacgcaagca cggcaaaaac gcctactacg gccaaaatta aggggttaca
     2221 atgagtaaaa attttaacaa aatttgatta ctaatttcaa ttttcacagt tgcaacttgc
     2281 gcagttctct tcgcaagcac tttcgcaacc gccaaagaac aaccagtcac tgataatcct
     2341 acaacctata cagtatcggg taaagttgtt gatacaaata gtgctgctct gcaaaatgtt
     2401 ttagcttcgg ggagcttttt taaggaaagc ggcgaggctt acattacgca agatgcatac
     2461 actggcgaag atggtacatt tacaattaat ggagtccttg aaggttcata tggagcaatc
     2521 tcattttacc ctgctgaagg agacactaca cactctggtg taaagagtta cattgatgga
     2581 cctatcatat ctaattataa tattggagaa gcgaagttgc cttcgttaac aaatcaagta
     2641 agtggtaagg ttactgatgt aaatggtgct ggtataccaa atgttacact tgagttttac
     2701 aatccattta gcgttaagtc tccttttgta gacattgctg ctaaaacaga tgccgaagga
     2761 aattattcgc ttgagagccc aattgtctct tcagattatt acagtggagt atcaattggg
     2821 agtgattatt atgttgttgc tcaaactgga gaatgtgaaa aaggttattt atcaaaaacg
     2881 cttgatttgc cagaagtaca ggaaagtgca aatgtattaa attttcaact tgatagaatt
     2941 gtaaatttga aattatatga ttatgatcaa accgaaaatg gctgggcaga atggtaccat
     3001 gagctctaca ttaatggaga actacaaagt gaaggaagct atctaggcga ttcaaatttt
     3061 caacttcctc taaatgcaaa atttacgttc aaagataatt gcaaacttga acttgcttac
     3121 actcttggcg aagatgaata tcttgatact tttgtcccag cttttaggaa tgctattaaa
     3181 tgggaaggtc aagactttaa tgaaaacctc attggtgatt attggatgtt ttggattaat
     3241 gaaaaatctg agggcactcc gccaattgag tgcagagatg gttactctta tcagtgcgac
     3301 aattccacaa attatttgta tttagatgtg aattttaaag caaagaatgt tgagccagcg
     3361 gttgttacgc cagcacagac aggtgatgaa aatggcatgt tgattgcaat tttaattgct
     3421 gttgtgg
//
LOCUS       DBNCDC010000105         3367 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_21084_length_3367_cov_7.686292, whole genome shotgun sequence.
ACCESSION   DBNCDC010000105 DBNCDC010000000
VERSION     DBNCDC010000105.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21084_length_3367_cov_7.686292"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(1..557)
                     /locus_tag="AC04VQ_06770"
                     /pseudo
     CDS             complement(1..557)
                     /locus_tag="AC04VQ_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306336.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
     gene            complement(551..1000)
                     /gene="rplO"
                     /locus_tag="AC04VQ_06775"
     CDS             complement(551..1000)
                     /gene="rplO"
                     /locus_tag="AC04VQ_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015539819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIO9553803.1"
                     /translation="MELIDLKPAEGSRHAKKRVGRGIGSGRGKTSGRGMNGQMSRAGG
                     GKRAGFEGGQNPLARRLPKLPGFRNFNRTEYVPVNVSRLEEKYKAGEVVDVDSLAKYG
                     IIKSTDCVVKVLGSGDIKKKLTVKVDKVSASAKAKIEKAGGKVEEPC"
     gene            complement(1000..1182)
                     /gene="rpmD"
                     /locus_tag="AC04VQ_06780"
     CDS             complement(1000..1182)
                     /gene="rpmD"
                     /locus_tag="AC04VQ_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIO9553804.1"
                     /translation="MAKKLVITQVGSEISRPESQAKTLKALGLGKIGRSVEQPDNEAI
                     RGMIFKVKHLVKVEEK"
     gene            complement(1182..1766)
                     /gene="rpsE"
                     /locus_tag="AC04VQ_06785"
     CDS             complement(1182..1766)
                     /gene="rpsE"
                     /locus_tag="AC04VQ_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIO9553805.1"
                     /translation="MAQKTQRREAHVDELEERVVHINRVAKVVKGGRRFGLTALVVVG
                     DRNGKVGIGMGKSQEVPIAVNKAVSEAKKNMFKVSLTPEKTVPHEIMGEYGAGRVLIK
                     PATPGTGIIAGSAARAILELAGVTDVFAKSLGTDNIMNVVKATADGLKNMESPNQVSR
                     RRGMSVQKIFGIKEKKQAAPKDEKKPAAKKEADK"
     gene            complement(1781..2149)
                     /gene="rplR"
                     /locus_tag="AC04VQ_06790"
     CDS             complement(1781..2149)
                     /gene="rplR"
                     /locus_tag="AC04VQ_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIO9553806.1"
                     /translation="MKKLVKKQQGLKRRHTRVRGKVEGTAKCPRLCVTRSNKNLYVQF
                     VDDVAGKSICGVSTHSADFKKTKKNGATVEGAAALGKIAAKAAQDKKIKKVVFDRGGN
                     LYHGRIKALAESCREGGLEF"
     gene            complement(2151..2696)
                     /gene="rplF"
                     /locus_tag="AC04VQ_06795"
     CDS             complement(2151..2696)
                     /gene="rplF"
                     /locus_tag="AC04VQ_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIO9553807.1"
                     /translation="MSRVGNNPIPIPDGVEVNIEGCSVTVKGPKGELVREFNKDMKIE
                     KDENTVVVKRPNDQREVMAMHGLTRSLLNNMVEGVSKGFEKKLELTGVGYRAQIKGRD
                     LELQLGYSHPVVIKCPEGITFESPSQEEIFVKGIDKQLVGEIAANIRKQRPPEPYKGK
                     GIKYEGEHIRRKLGKAAKTEA"
     gene            complement(2708..3106)
                     /gene="rpsH"
                     /locus_tag="AC04VQ_06800"
     CDS             complement(2708..3106)
                     /gene="rpsH"
                     /locus_tag="AC04VQ_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIO9553808.1"
                     /translation="MSMTDPIADMLTRVRNANQAGHEKVSMPSSKKLVEIARIMKEEG
                     FVAGYDVVKSQPVDKLEVTLKYGDNKDKTIRGIKRISKPGLRIYAGKDDLPRVLGGLG
                     CAIISTSSGVMTDRDARQKGVGGEVMAYIW"
     gene            complement(3127..3312)
                     /locus_tag="AC04VQ_06805"
     CDS             complement(3127..3312)
                     /locus_tag="AC04VQ_06805"
                     /inference="COORDINATES: protein motif:HMM:NF005974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIO9553809.1"
                     /translation="MAKKSMIAKAKKPAKYATRQHNRCSRCGRPRAYYRKFDMCRVCV
                     RELANKGELPGVTKSSW"
BASE COUNT          946 a          848 c          566 g         1007 t
ORIGIN      
        1 gacataccat taccaatacc atgctgagtg ataagttcac ccatccacat gataaacgca
       61 gtaccagcaa tcaaagtaaa tataattact acgcttgtca accaaacagg aacacccata
      121 gattcttcgt taaattgaac attatattgg ctagactgga acaaaagcaa ataaccaata
      181 gcattaataa gaccaagaac aagtgttaaa taacgagtcc actgagtaat tttcttttgg
      241 ccactttcgc cttctttagc tcaacgacca acagcaggaa taacgccttg cataagctgc
      301 ataataatgc ttgctgtgat gtatggcata atcccaagcg aaaataccga aaaacgcgac
      361 agcgcaccac cagtaaacaa gtccaacatg ctcatggcaa caccactgtc ttgaaaacta
      421 tcggcaaaac ccttgaaagg gatacctgga actgggataa aagctccaag tctgtaaagt
      481 gcaagaatag ctaacgtaaa taaaatttta ttacgaagct ccctgacttt gaatgcatca
      541 attaatgaac ttaacaaggc tcttcaacct ttcctccggc tttctcaatt ttagcttttg
      601 ctgaagccga aaccttatca acttttactg tcaatttttt cttgatatct ccacttccaa
      661 gaacctttac aacacagtca gtgcttttaa taattccata ttttgcaagt gaatcaacat
      721 caacaacttc gccagcttta tatttttctt ccaaacgaga aacattaact ggaacatatt
      781 ctgttcgatt aaagtttcta aagccaggaa gttttggcag gcgacgtgca agtggatttt
      841 gaccaccctc aaaaccagcg cgctttccgc cgccagcacg agacatttgg ccattcatac
      901 ctctaccact tgttttgcct cgaccactac caataccgcg accaacacgc tttttagcgt
      961 gacgagaacc ttcggcaggt tttaaatcta taagttccat tatttctcct ccaccttaac
     1021 aagatgctta actttaaaaa tcattccacg aattgcttcg ttgtcaggtt gttcaacgct
     1081 gcgtccaatc ttgccaagac ctaaagcctt cagtgttttg gcttgagatt ctgggcgaga
     1141 aatttcgctg ccaacctggg taattactaa cttcttagcc attatttatc agcctccttt
     1201 ttcgcagcag gcttcttttc atctttaggt gcagcttgtt tcttctcttt aattccgaag
     1261 atcttctgca cgctcattcc gcgtctacga gaaacctgat ttgggctttc catattttta
     1321 agaccatcag ctgtagcctt aacaacgttc ataatattgt cagtgcctaa gctcttggcg
     1381 aaaacatcgg taactccagc aagttccaaa atagcacgag cagcagaacc tgcgataatt
     1441 ccagtaccag gtgttgctgg cttaattaaa acgcgtccag caccatattc gcccatgatc
     1501 tcatgaggga cagttttttc aggagtcaaa gaaaccttaa acatgttttt ctttgcttca
     1561 gaaactgctt tattaacagc aattggaact tcttggcttt tacccatacc aattccaact
     1621 ttgccattgc gatctccaac aacaacaaga gcagtcaagc cgaagcgacg gccacccttt
     1681 acaaccttag caacgcggtt aatgtgaact acgcgctctt cgagttcatc aacatgtgct
     1741 tctcttcttt gagttttttg agccattatc aatttccttt ctaaaactct aatccaccct
     1801 cacggcaact ttcggccaat gccttaatgc gtccgtgata aaggttgcca cctcgatcga
     1861 aaacaacctt tttgattttc ttatcttgtg cagctttagc agcaattttc ccaagagcag
     1921 cagcgccttc aacagttgca ccgtttttct tggtcttttt aaaatctgct gaatgagttg
     1981 aaacaccaca tatagatttt ccagcaacat catcaacaaa ttgaacataa agatttttat
     2041 tgctacgagt aacacacaaa cgcgggcact tcgctgtgcc ttcgacctta ccacgaacac
     2101 gtgtgtgacg acgttttaaa ccttgttgtt tcttaactaa ctttttcata ttaggcctcc
     2161 gtctttgctg ccttaccaag cttgcggcga atgtgttcac cttcgtactt gataccttta
     2221 cccttataag gctctggagg acgttgcttg cgaatgtttg cagcaatctc tccaaccaat
     2281 tgcttatcaa tgcctttgac aaaaatctct tcttgcgaag ggctctcgaa agtaattcct
     2341 tcagggcact ttataactac aggatgcgaa tatccaagtt gaagttcgag gtcacggccc
     2401 ttgatttgag ctctgtaacc aacacctgta agctcaagct tcttttcgaa gcccttgcta
     2461 acgccttcaa ccatattgtt tagcaaacta cgagtaagac catgcatagc cattacttcg
     2521 cgctgatcat taggacgctt aacaacaact gtattttcat ccttctcaat tttcatatct
     2581 ttattaaact cgcgaacgag ttcaccctta ggtcctttaa cagtgaccga acaaccttcg
     2641 atgttaactt caacaccgtc aggaattgga atagggttat ttcctacacg tgacatctca
     2701 taacctccta ccaaatatag gccataactt cgccaccaac gcctttttga cgggcatcgc
     2761 ggtcggtcat aacaccactg cttgtagaga taattgcgca acccaggccg ccaagaacac
     2821 gaggaagatc atccttaccc gcataaatac gaagaccagg ttttgaaata cgcttaatac
     2881 ctctaattgt cttatctttg ttatcgccat acttcaatgt aacttcaagc ttgtcaactg
     2941 gctgactctt tacaacatca tatccagcaa caaagccctc ttccttcata atgcgagcaa
     3001 tttcaacaag ctttttagaa cttggcatgc taactttttc atgcccagct tggttcgcat
     3061 tacgaacgcg agttaacata tctgctattg gatcagtcat actcatttac atcacctcta
     3121 tctatcttac caagacgact ttgttacgcc tggtaattca cccttatttg caagttcacg
     3181 aacacataca cggcacatat cgaatttgcg gtaatatgcg cgtggacgtc cgcaacgact
     3241 gcagcgatta tgctgacgag tcgcatactt tgcgggtttt tttgcttttg ctatcataga
     3301 ttttttagcc atggttttcc tctcttacca tcttttattt ctcggcgaat gggaagccca
     3361 acatttg
//
LOCUS       DBNCDC010000106         3353 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_21237_length_3353_cov_6.021225, whole genome shotgun sequence.
ACCESSION   DBNCDC010000106 DBNCDC010000000
VERSION     DBNCDC010000106.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21237_length_3353_cov_6.021225"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..104)
                     /locus_tag="AC04VQ_06810"
     CDS             complement(<1..104)
                     /locus_tag="AC04VQ_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase preATP-grasp
                     domain-containing protein"
                     /protein_id="HIO9553810.1"
                     /translation="MPKRTDIKKILVIGSGPIVIGQAAEFDYAGTQACL"
     gene            complement(97..645)
                     /locus_tag="AC04VQ_06815"
     CDS             complement(97..645)
                     /locus_tag="AC04VQ_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase-related protein"
                     /protein_id="HIO9553811.1"
                     /translation="MGITSHNLDDLNKAGMNVTVVPYDTSVEKIKSYRPDGIFLPKGP
                     GNPKNAKHVIEVVKKLQGKYPIMGISLGLQILALANGLDIKKMKVGHRGSNHAVRNEK
                     TGKLFVVNQSHGYVIKDFKKKGIEVTCRDIMDNTIEGLDIPAKKAFGVQYYPNRTANP
                     ADSKAILDKFKAYMGSKGGRHA"
     gene            complement(691..1179)
                     /locus_tag="AC04VQ_06820"
     CDS             complement(691..1179)
                     /locus_tag="AC04VQ_06820"
                     /inference="COORDINATES: protein motif:HMM:NF013178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HIO9553812.1"
                     /translation="MKRKIVFENGREFVGEAFGSTKDVVSEVVFNTNMVGYQEILSDP
                     AYTDLSILMTYPLIGNYGIIDEDFDSKLIGVKALITSEYNDKPSNFRYANTLAETLKE
                     NDVAGVTHVDTRAITRMLVREGVMKAAICDEKTSKATAMKRIKNYKAPKDQIKRVSCK
                     KV"
     gene            complement(1180..2493)
                     /locus_tag="AC04VQ_06825"
     CDS             complement(1180..2493)
                     /locus_tag="AC04VQ_06825"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIO9553813.1"
                     /translation="MRQIANIYPDHQRGVGPNGHAPGYGGRCEVLRLFTGSTILIQGK
                     FVASDFIINNFNFELYNPDKHSNLKFDEVIDCSGLHVFPGLIDPHVHLREPGFEYKDT
                     IKTGTMSAAKGGYTTVFAMPNLNPVPDTVENLQVEKDAIERDAVINVYPVCAITKGQT
                     GRGELADIDNLKQENKLFSDDGKGVQAGVNMEKAMTKIAAANGYVLAHCEDEDELARG
                     DSASAEYVQVKRDLELSDKTGCKYHVCHMSTKQSVEALRQHKNENVSGEVTCHHLFLN
                     ENDVKDNGKWKMNPPLRSTEDQKAVVDGLLDGTIEMICTDNAPHSADEKDCPFDDAAM
                     GIVSIEVAFPLVYTNLVKTGKLTLERTIELMSTNAAKFFEIPGGEIVENKAANICIYD
                     LSEDWTIDAGNFASLGKSCPFDGQKVSAKCKMTIANGEIVFREMD"
     gene            complement(2400..3278)
                     /locus_tag="AC04VQ_06830"
     CDS             complement(2400..3278)
                     /locus_tag="AC04VQ_06830"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIO9553814.1"
                     /translation="MKDLLTLKELSVDEIQSILDLALECEKGKYDNFLKDKIIANCFF
                     EPSTRTQYSFNTAELRLGMKPLSFNPAGSSLNKGESFYDTIKTFESFGISGIVIRDVH
                     DRYYEDIRHLDFHILNAGDGAGDHPSQSLLDLLTIRKEFGKFEGLKIAIVGDISHSRV
                     AHTNIEVMERLGMECFVSGPEEYREEGYNFIDFDKAVEEMDIIMLLRVQHERHKEEMQ
                     ISLEDYNKQYGLNMDRVNKMKDHAIIMHPAPFNRGVEINDDVVECDKSRIFTQITNGV
                     LVRMAMLLVTVGGVKC"
BASE COUNT          889 a          750 c          658 g         1056 t
ORIGIN      
        1 aggcaagctt gagttccagc ataatcaaat tctgcagctt gaccaataac aatagggccg
       61 ctgccaataa ctaaaatctt tttaatgtcc gtgcgtttag gcatggcggc ctcctttcga
      121 tcccatatag gctttgaatt tgtctaaaat agccttgctg tctgcagggt tggccgtccg
      181 gttagggtag tattgaactc cgaaagcctt cttcgccggg atgtcaaggc cttcgatagt
      241 gttatccata atgtctctac atgtaacctc gatacctttc tttttgaaat ctttaattac
      301 ataaccgtgg ctttggttaa caacgaagag cttgccagtc ttttcgtttc gaacagcgtg
      361 atttgagccc ctgtgaccaa ccttcatctt tttaatatcg aggccatttg caagagccaa
      421 aatttgaagc ccgagtgaaa ttcccatgat tggatattta ccttgaagct ttttaacaac
      481 ttcaattacg tgttttgcgt tcttagggtt accaggccct ttaggcagga agatgccatc
      541 tggcctatag gacttaattt tttcaacact tgtatcatat gggacaacag ttacgttcat
      601 gccagcctta ttaaggtcat ctaagttatg gcttgtaatt cccatatcaa ttgcaagaac
      661 attgtattgc gggttagcgc atcgcgaata tcaaactttc ttgcaagaca cacgcttgat
      721 ttgatccttt ggagccttgt aatttttgat gcgtttcata gccgttgctt tagaagtttt
      781 ctcgtcgcaa attgcagctt tcatgacgcc ttcgcgaaca agcatgcgag taattgcacg
      841 agtatcaacg tgtgttacac cagcaacatc gttttctttc aaggtctctg caagagtatt
      901 tgcgtaccta aaattggaag gcttgtcgtt gtattcgctt gtaattagag ctttaactcc
      961 aataagctta ctgtcgaagt cttcgtcaat aattccgtaa tttccaatta gcgggtaggt
     1021 catcaagatg ctaaggtcgg tgtaagcagg gtcgcttaga atttcttgat agccaaccat
     1081 gtttgtgtta aagaccactt cagaaacaac atctttagtg cttccgaagg cctcgccaac
     1141 aaactctcga ccgttttcaa aaacaatctt ccttttcatt tagtccatct ccctaaaaac
     1201 aatttctcca tttgctattg tcattttaca tttagcactc actttttgac catcgaaagg
     1261 acagcttttt ccaaggcttg caaagtttcc agcatcgata gtccagtctt cgctgagatc
     1321 gtaaatgcaa atgttagcag ctttattttc aacaatttcg ccgccaggaa tttcgaaaaa
     1381 tttggcagcg ttagtactca taagttcaat tgttcgctcg agcgtgagct ttccagtttt
     1441 aaccaaattt gtatagacaa gagggaaggc cacttcgatt gacacaattc ccattgcagc
     1501 gtcatcaaat gggcagtctt tttcatcggc gctatgaggt gcgttgtctg tgcaaatcat
     1561 ctcgatcgtg ccgtccaaaa gtccatctac aacagctttt tggtcctcgg tactccgaag
     1621 cggagggttc atcttccact tgccattatc cttgacatcg ttttcattta agaacaaatg
     1681 gtggcatgta acttcgccac taacgttttc atttttatgt tgacgaagcg cctcaacgct
     1741 ctgctttgta gacatatgac aaacgtgata tttacaaccc gttttatccg aaagttctaa
     1801 atcacgcttg acttgaacat attctgcaga tgcactatca ccgcgtgcga gctcgtcttc
     1861 gtcttcgcaa tgtgctaaaa catagccatt tgcagcagca attttagtca ttgctttttc
     1921 catgttaacg ccagcctgaa caccttttcc gtcatccgaa aagagcttat tttcttgctt
     1981 taagttgtca atgtccgcaa gttcgcctcg tccagtctgg ccttttgtga tagcgcaaac
     2041 agggtagacg ttaataacag catcgcgctc aattgcgtct ttttcaactt gcaagttttc
     2101 gacagtatca ggaacaggat tcaaattagg cattgcaaaa acagtcgtat aaccaccttt
     2161 agcagcagac atagttccag tctttattgt gtctttgtat tcaaaacccg gctcgcgaag
     2221 gtgaacgtgt gggtcaatta gcccaggaaa cacgtgaagg ccggagcagt ctattacttc
     2281 gtcgaatttg agatttgaat gtttatcagg attgtaaagc tcaaaattaa aattattgat
     2341 tatgaagtct gaagcgacaa atttaccttg gataagaata gtggagccgg taaaaagtct
     2401 tagcacttca cacctcccac cgtaaccagg agcatggcca ttcggaccaa caccccgttg
     2461 gtgatctggg taaatattcg cgatttgtcg cattcgacca cgtcatcgtt gatctcaaca
     2521 cctctgttaa atggcgcagg atgcatgatt attgcgtggt ccttcatttt attaacgcga
     2581 tccatgttta gaccgtattg tttattgtaa tcttcgaggc taatttgcat ttcttcttta
     2641 tggcgttcat gctgaacacg aagcaacatt attatgtcca tctcttcaac cgctttgtcg
     2701 aagtcgatga agttgtatcc ctcttcacga tattcttcag gtccagatac gaagcattcc
     2761 atacccaggc gctccattac ctctatatta gtatgagcaa cacgcgaatg acttatgtca
     2821 ccaacaatcg caatctttaa gccttcaaat ttcccaaatt cctttcgaat tgttagcagg
     2881 tcaagaagcg attgggaagg gtggtcgcca gcgccatcgc cagcatttag aatgtgaaag
     2941 tcaaggtggc gaatgtcttc gtaataccta tcgtgaacat cgcggatcac aataccagaa
     3001 attccgaagc tctcaaaagt cttgattgtg tcataaaaac tttcgccctt atttaagctg
     3061 ctacctgcag gattgaaact tagtggcttc attccaagac gaagctcagc agtgttgaag
     3121 ctgtattgag ttcttgtgct tggttcaaaa aagcagttag caataatttt gtcttttagg
     3181 aaattgtcat acttgccctt ttcacattca agggctaaat ctagaatact ttggatctcg
     3241 tcgacgctta attcctttag cgttagcagg tctttcattt agctctagcc tccaaactaa
     3301 atcaatccca aagttgtaga tataataaca caacttgaga gattttgaat atg
//
LOCUS       DBNCDC010000107         3220 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_22767_length_3220_cov_6.175987, whole genome shotgun sequence.
ACCESSION   DBNCDC010000107 DBNCDC010000000
VERSION     DBNCDC010000107.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22767_length_3220_cov_6.175987"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="AC04VQ_06835"
     CDS             <1..596
                     /locus_tag="AC04VQ_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015539805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIO9553815.1"
                     /translation="GVDHVLASGKNINIMVFDTEVYSNTGGQASKASNLGQVAQFAAA
                     GKEVAPKSLAHMAMTYGYVYVAQVAMGANQAQTIKAIKEAEAYNGPSLIIAYSPCEMH
                     AIKGGMVNCHTEMKKAVDCGYWNLFRFNPAAEDKCLTIDSKEPGEGYQDFLMGEARYN
                     RLTREFPERAEKLFAQNEQKSKERYELLKRLEQVYTR"
     gene            657..1403
                     /locus_tag="AC04VQ_06840"
     CDS             657..1403
                     /locus_tag="AC04VQ_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553816.1"
                     /translation="MDEKLIEKNRELVERRLDKGLEIIKKRFEVEEIPIRDALNNFTL
                     DGRTYSAFQYDIKEIGNMYYMKSTNKDDFMMDTMIFTPYYKHLPVFSTDYLFMVDQRN
                     VLHELYDLVDEEYITDTYKEYVKKFNDNLKRYDYVENKPPHNPWYEYLRSFYLAKLPT
                     PEQDDDMYVAFEENMNLFLDMAEATPLIKTKEEYEKKWKQNKWYSDSLLERAGIATQV
                     FLDNVGQDFTDNFFEECFFAPGTYKDKFNN"
     gene            1465..2160
                     /locus_tag="AC04VQ_06845"
     CDS             1465..2160
                     /locus_tag="AC04VQ_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIO9553817.1"
                     /translation="MRLLVVEDKKALANIVGERLEKEGFSVDVVYDGEEGLYCAASGA
                     YDLIILDIMLPGLDGFEVLEELKKKKVDSKVIILSARSALSDKLKGLQNGADDYVTKP
                     FHLDELVARVELQLKDRTGAVDGSLNFGDLSLDAKTSILKCTKSKQEVELVKKELEIL
                     EFLINNRKQIVSKQQIYDNVWGIENEVESNNLEVYLSFIRRKLKAIGSKTNIKASRGL
                     GYKLEYSDEVAKN"
     gene            2450..2842
                     /locus_tag="AC04VQ_06850"
     CDS             2450..2842
                     /locus_tag="AC04VQ_06850"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIO9553818.1"
                     /translation="MADASHELKTPLSVVMASADAISPTAKNKKYLENIKEESSRMNS
                     LIAKLLDLASTEKTDENYLTEGNLSKTVELTALTFEAKALERNRKISIDIEENIMLKQ
                     NVNDIKQLVEILLDNALEHSIEKSTINL"
     gene            2861..3079
                     /locus_tag="AC04VQ_06855"
                     /pseudo
     CDS             2861..3079
                     /locus_tag="AC04VQ_06855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         1055 a          559 c          659 g          947 t
ORIGIN      
        1 gtggtgttga tcacgttttg gcttctggca aaaacatcaa cattatggta ttcgacactg
       61 aggtttactc aaacactggt ggtcaggctt caaaagcttc aaacttaggc caggttgcac
      121 agtttgcagc agctggtaaa gaagttgctc ctaagagctt agctcatatg gctatgactt
      181 atggttatgt ttatgttgct caagttgcta tgggtgctaa ccaggctcaa acaattaagg
      241 caatcaaaga agctgaagct tacaacggac cttcgcttat cattgcttat tcgccatgcg
      301 aaatgcatgc aattaagggc ggtatggtta attgccacac tgaaatgaag aaggctgttg
      361 actgcggtta ttggaactta ttccgcttta acccagccgc tgaagataag tgcttaacta
      421 tcgattcgaa agaaccaggt gagggctatc aagatttctt gatgggcgag gctcgctaca
      481 atcgtttgac tcgtgaattc ccagagcgcg ctgagaagtt atttgctcaa aatgaacaaa
      541 aatctaaaga acgctatgaa ttactaaagc gtttagagca agtttataca cgatagttaa
      601 acttaaagag cccggcataa aatttgtcgg gctctttgtt ttttaagggg taaatcatgg
      661 atgagaaatt aattgaaaaa aatagggaac tggttgaaag aagacttgat aaaggtctcg
      721 aaattatcaa gaagcgtttc gaagttgagg aaatcccaat cagagatgct ctaaataatt
      781 ttactttgga tggacgtaca tattcggcat tccagtacga tattaaagaa attggaaata
      841 tgtattacat gaaaagtacc aacaaagatg atttcatgat ggacactatg atttttactc
      901 cgtattacaa acatctacct gtattttcga ctgattatct ttttatggtt gaccagcgca
      961 acgttttgca cgaactttat gatttagttg atgaagaata catcactgat acttataaag
     1021 aatatgtaaa gaagtttaat gacaatctta agcgttatga ctatgtcgaa aacaagccgc
     1081 cgcataatcc ttggtatgaa tatcttcgtt cgttctattt agcgaagctt ccaactccgg
     1141 aacaagacga tgatatgtac gtggctttcg aagagaacat gaatttattc cttgatatgg
     1201 cagaagctac accacttatt aagaccaagg aagaatacga aaagaaatgg aagcaaaaca
     1261 aatggtattc tgacagtttg cttgagcgtg ccggaattgc tactcaagtg ttccttgata
     1321 atgtggggca ggacttcact gacaatttct tcgaggaatg cttcttcgcg ccaggaactt
     1381 acaaagataa atttaataat taactatttc tcatttaatg tttatttaat ctcgttttaa
     1441 tatattataa gcgaggtgat aaacatgcgt ttattagttg tagaagataa aaaggctctt
     1501 gccaatattg taggagagcg acttgagaaa gaaggcttct ctgttgatgt agtttatgac
     1561 ggcgaagaag gcctttactg tgcagcaagt ggcgcctacg accttattat tcttgacata
     1621 atgctacctg gccttgatgg ctttgaggtt cttgaggaac ttaagaagaa aaaagttgat
     1681 agcaaagtca taattttatc cgcgagaagt gcactaagcg ataaactcaa aggccttcaa
     1741 aacggcgctg atgactatgt tactaaaccg tttcatcttg atgagcttgt tgcaagagtt
     1801 gaacttcaac ttaaagatag aactggcgcc gtagacggct cgcttaattt tggcgactta
     1861 tcacttgacg ctaaaacatc tattttaaaa tgcacgaaaa gtaaacaaga agtagaactt
     1921 gttaagaaag agttagagat tcttgaattt ctgattaata ataggaaaca aatagtctct
     1981 aaacaacaaa tttatgacaa tgtgtggggg atagaaaacg aagttgagtc aaacaacctt
     2041 gaagtttatt tgtctttcat tcgtcgaaaa cttaaagcga ttggatcaaa gactaatatt
     2101 aaagcgtcaa gaggtctagg ttataaattg gagtattcgg atgaagtcgc taagaactaa
     2161 agttttttga gcaatctacg gctcgctaag tttggttgtg tttatatcta tattgatata
     2221 caacatctct aattatttta cgacaagaaa catgctccat gatgctgcgc gctcggtttt
     2281 tgttgaccca agatatactg actttgtcat tcaaggcttc attaataatc ttttgacttc
     2341 gcttatattg ttaataatag ttctggtagt attatatttc gttgtcaaaa aattgactga
     2401 ttgaattgtt aggcctacag aagagtcttt cgcaaagcaa caagatttta tcgccgacgc
     2461 atcgcatgaa ctaaaaactc ctttatctgt tgtgatggct tcggcagatg caatttctcc
     2521 aacggctaaa aataaaaagt acttagaaaa tattaaagaa gaatcatcgc gcatgaattc
     2581 cttgatagcg aagcttcttg atcttgcatc tactgaaaag acagatgaga attatctcac
     2641 tgaaggcaac ctatcaaaaa cagttgaact aacggcattg acattcgaag caaaggcgct
     2701 tgaacgaaat cgtaagattt cgatcgatat tgaagaaaat attatgctca aacaaaatgt
     2761 aaacgacatt aagcaactag ttgagatttt acttgataat gcactagagc attcaataga
     2821 aaaatctaca atcaatttat gactacataa agaaggcaag caaattgttc ttgaagtcat
     2881 taacgaaggt gagggcattg ctccgggaga cgaagaaaaa atcttcgagc gtttctatcg
     2941 cgccgacaaa tcacggtcag gtgagaacaa ccgctacggc ctcggcctcg ccatcgctaa
     3001 aaatatagca gaaaatcata acggcaaaat cactgcaaaa tcacaaaaca acaaaaccac
     3061 attcaaagtt ttcctttaat atgatatact cgcgttgtca agttaaagaa aggaattcct
     3121 atgatgtttc aactttatgg cccgacgtct ggctggcaat tgcttggctg gctgatggtt
     3181 tttgttggct tagttttagt caacgaattt gcgcgccgca
//
LOCUS       DBNCDC010000108         3058 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_24994_length_3058_cov_8.319347, whole genome shotgun sequence.
ACCESSION   DBNCDC010000108 DBNCDC010000000
VERSION     DBNCDC010000108.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24994_length_3058_cov_8.319347"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(1..1221)
                     /locus_tag="AC04VQ_06860"
     CDS             complement(1..1221)
                     /locus_tag="AC04VQ_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIO9553819.1"
                     /translation="MALNVKVGLIGLLDMLKFKKMGQAREDWVQHAPANEIVTTFPIN
                     VNAKKLHPDYQDLVVDKVVDYDKAGAKMFVFKRQGGGNPANFRAGQYLSLKLEIDGSA
                     VTRPYSICSTPKEATQEGKVALTIKTNPGGFVADHLLENLKKGDRVITSDPQGQFYYE
                     DLRDAKNVIALAGGSGITPFLSMAGAIVEGTEDFNLTILFGSRTEEVILFKDELEKLE
                     KASNGKVKVVHILSDEEKDGYEHGFISAEIIKKYAPADDDYSIFVCGPEAMYKFAEKE
                     VAKLKLPKRLVRNELLGVTKDVTKDPDYKGKADKTYTITVKQAGQKEVQIPAAANETL
                     LVAFERAGIKAPSRCRSGECGWCRSQLTDGKVFIPKATDGRRESDKRWNTIHPCATFP
                     LSDCTVEVPGEYWK"
     gene            complement(1221..2498)
                     /locus_tag="AC04VQ_06865"
     CDS             complement(1221..2498)
                     /locus_tag="AC04VQ_06865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIO9553820.1"
                     /translation="MDVTMPHGIDNFINAMEEYVPGSKPKMEELFELFDEVLAGIGYI
                     SSANGNPDSNVLKEKYPNLLRTGAYPTQKVFDALKLPKRCQDILQTYWGYLGTDMEHL
                     SFIHYAAMVHKYVSRGAYIPTYTSHEISSAFIERFRELGGEVWYNCRLEKILFDGDKV
                     CGVETSLGKVECDFCLPNVNSNIVYGKMVPKELVPERMKKLSTARKATFGGRMMTAYF
                     CLDKTPEELGIKDYSIFFAGGCDSLKEYNGMLEGGDGNQFVIFLCYNIANPKASPDGT
                     CICSFTSFASPNDWNDLSQEDYYKYKNTWANKWLDRLRDEAGIDLRGHIEEMSVASPW
                     TFARYLGNPEGSVYGHETRDADSMMARLQSLGTDYPLKGLRPIGADGPRGDGYSAAYI
                     CGQLMANLATKDMKEWEEAAKAPVAEESKGGDA"
     gene            complement(2517..2810)
                     /locus_tag="AC04VQ_06870"
     CDS             complement(2517..2810)
                     /locus_tag="AC04VQ_06870"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIO9553821.1"
                     /translation="MAKKYDAVVCGAGNSGISAAVQLALNGKKVLLIEQHNMPGGSAS
                     SFRRGRFEIEPSLHELCDVGPESDPGDVRNLFDSYGIKIEWNECPDCFRVISK"
     gene            complement(2909..>3058)
                     /locus_tag="AC04VQ_06875"
     CDS             complement(2909..>3058)
                     /locus_tag="AC04VQ_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIO9553822.1"
                     /translation="DECTACGACVDACPCGVLEMGDVATVANPDECTECGTCVDECPV
                     GAITL"
BASE COUNT          913 a          728 c          556 g          861 t
ORIGIN      
        1 ttacttccag tattcgcctg gaacttcaac cgtgcaatct gaaagtggga atgttgcgca
       61 aggatggatt gtgttccaac gcttgtcaga ttcgcggcgt ccatcagtag cctttggaat
      121 aaaaactttt ccatcggtta attgagaacg acaccaaccg cactctccag aacgacaacg
      181 tgaaggagcc ttaattccag cacgctcaaa tgcaacaaga agtgtctcat ttgcagcagc
      241 aggaatttga acttctttct ggccggcttg cttaacagta attgtataag tcttgtcagc
      301 cttgccctta taatcaggat ccttagttac atcttttgta acaccaagaa gctcgttacg
      361 aacaagacgc tttggaagct taagcttcgc aacttctttt tccgcaaatt tatacatagc
      421 ctctggacca caaacaaaga tgctgtaatc atcatcagca ggagcatatt tcttaatgat
      481 ttcagcacta ataaagccat gctcataacc gtctttttct tcgtcagaaa gaatgtgaac
      541 aactttaact ttaccgttcg aagctttttc aagtttttca agttcatctt tgaaaagaat
      601 aacttcttcg gtgcggctac caaaaagaat tgttaagttg aagtcttcgg tgccttcaac
      661 aattgcgcca gccatgctaa ggaatggagt aataccacta ccaccggcca aagcaataac
      721 gtttttagca tcacgaaggt cttcataata gaattgacct tgagggtccg aagtgattac
      781 gcgatcaccc ttcttcaaat tctcaagaag atgatctgca acaaatccgc ctggatttgt
      841 cttaatagta agtgcaacct taccttcttg tgttgcttcc ttaggtgttg agcaaattga
      901 ataaggacga gtaacagccg aaccatcaat ttctaacttc aaagataggt actgaccagc
      961 acggaagtta gcaggatttc cgccgccttg acgcttgaat acaaacatct tagcgccagc
     1021 cttatcataa tcaacaacct tatcaactac aaggtcctga taatctggat gcaatttttt
     1081 tgcatttaca ttaattggga aggtggttac aatctcgttt gctggagcat gttgaaccca
     1141 gtcttcacgt gcctggccca tcttcttaaa tttcaacata tctaaaaggc caatcaagcc
     1201 aacttttacg ttcaaagcca ttatgcatca ccacctttcg actcttcagc aactggtgct
     1261 ttagctgctt cctcccattc tttcatatct ttcgtagcga ggttagccat aagctgacca
     1321 caaatgtaag ctgcactgta gccatcacca cgaggaccat cagcaccaat tggacgaaga
     1381 cccttcaatg gataatcagt tccaagtgat tgtaaacgag ccatcatgct gtcagcatcg
     1441 cgagtctcat gaccataaac tgaaccttct ggatttccaa ggtaacgagc aaaagtccat
     1501 ggagaagcta ctgacatctc ttcgatatga ccacgaagat caataccagc ttcgtcacga
     1561 agtctatcaa gccacttatt tgcccatgtg ttcttatact tataataatc ttcttgtgaa
     1621 agatcattcc aatcatttgg agatgcaaac gaagtgaaag aacaaatgca cgtaccatct
     1681 ggcgatgcct taggattagc aatgttgtag cataagaaaa taacgaattg attgccatcg
     1741 ccaccttcta acatgccgtt gtattccttc aaagaatcgc aacctccggc gaaaaagatt
     1801 gaataatctt taatgccaag ctcttcagga gtcttatcaa gacagaaata tgctgtcatc
     1861 atacgaccac caaatgtagc tttacgtgca gtagacaact tcttcatgcg ttctggaaca
     1921 agctcttttg gaaccatttt tccataaacg atgtttgaat tgacgtttgg taagcagaaa
     1981 tcgcactcaa ctttaccaag cgaagtttca actccgcaaa ctttatcgcc gtcaaacaaa
     2041 attttctcaa gacggcagtt ataccaaacc tctccaccta gctcacggaa tctctcaata
     2101 aatgctgaag aaatttcgtg cgaagtgtaa gttggaatgt atgcaccacg gctaacatat
     2161 ttatgaacca tagctgcata gtgaatgaag cttaagtgct ccatgtctgt tccaaggtaa
     2221 ccccaataag tttgaaggat gtcttggcat cgcttcggaa gcttcaacgc atcaaaaact
     2281 ttttgagttg gataagcacc agtgcgaagc aagtttggat atttttcctt caaaacattg
     2341 ctgtcagggt ttccattagc cgaagaaata tagccaatac cagctaagac ttcgtcaaac
     2401 aactcaaaca attcttccat ttttggtttt gaaccaggaa catattcctc cattgcatta
     2461 atgaagttat caatgccgtg aggcatcgta acatccatgc gagaaccatc gtcacttcat
     2521 tttgaaataa cacggaagca gtctggacat tcgttccatt caatttttat accgtaagaa
     2581 tcgaataaat tacgaacatc acctggatcg ctttctggac caacgtcgca aagctcatgc
     2641 aaacttggtt caatttcgaa acgaccacga cgaaaactcg aagccgaacc gccaggcatg
     2701 ttgtgttgct caattaatag cacttttttg ccattaagag caagttgaac agctgcagaa
     2761 ataccactat taccggcacc gcaaacaact gcatcatact tttttgccat ttttcctcct
     2821 ttttaatttt ctcgattttg caattcacct agcatgcaag cgaacagcaa aagaaaaggg
     2881 cccgaagttc gagcccccat ttcttaattt aaagtgtgat tgcgcctact gggcactcat
     2941 ctacacaagt gccacattct gtgcactcat ctgggtttgc tacagtagca acatctccca
     3001 tttccaaaac accgcatgga catgcatcta cgcatgcgcc acaagctgtg cattcatc
//
LOCUS       DBNCDC010000109         2916 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_27320_length_2916_cov_6.926599, whole genome shotgun sequence.
ACCESSION   DBNCDC010000109 DBNCDC010000000
VERSION     DBNCDC010000109.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27320_length_2916_cov_6.926599"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            185..1522
                     /locus_tag="AC04VQ_06880"
     CDS             185..1522
                     /locus_tag="AC04VQ_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012372.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HIO9553823.1"
                     /translation="MAPNNNPTAEMQNSNFSLYDFCKRLSKNITGVYDKSCLDDDGHI
                     ISFNEQAAIITHYLHQQSDNIQLENKATELLRLWYSFFERDKKDSSMVHDNSDAFTGY
                     LFSECFNVPFRASLKPKFKFIDLFAGIGGFRIAMQNLGGECVFSSEWDEQAQKTYYTN
                     FGEVPFGDITKESTKKYIPQDFDILCAGFPCQAFSLAGKRRGFEETRGTLFFDVAEIL
                     KKHQPKAFFLENVKGLISHDKGKTLQVILNTLDEVGYIVPDPKIINAMYFGVPQHRER
                     IYIVGFRKDLGIKKEDFSYPEQKEISKHWIDVREENPVPAKYYLSTQYIQTLINHKAR
                     HEAKGHGFGYDIISDDGVSHAIVVGGMGRETNLVIDFRQTDLTPTTRIKGEVNKQGWR
                     KMTPREWARLQGFPDEFVIPVADASAYKQFGNSVAVPAIQATAEQLIKTLERN"
     gene            1527..2609
                     /locus_tag="AC04VQ_06885"
     CDS             1527..2609
                     /locus_tag="AC04VQ_06885"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021102.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpaII family restriction endonuclease"
                     /protein_id="HIO9553824.1"
                     /translation="MALSGNKGEWSEIYALFKLLSEGKVHAGDAEMNKMDLFYPIIDI
                     IREESKRYEYLPNVNQNIIIIHENGEEYGRISMDKFFLESKNLLNVLSKANDRAFEIP
                     STELFMNQIGCSKLKAPSKDKADIHIIIHDLHTNMQPLLGFSIKSQLGSASTLLNAGI
                     TTNFTFKITGKDLSDNDIANINNIYEHLPRMESIIKHGCKLEFVDVEHQTFKNNLLFL
                     DCCMPEFLALCLLADSMPNSTSSIKDIVKIVSTTNPFHFTGNDIESFYEHKMKVLLID
                     AALGMTPAKEWKGKYDANGGYLVVKNDGEIVCYHFYNRNDIEDYLFNNTRFERASRSR
                     YHFGTLYRGTDNCVYLKLNLQIRFKK"
     gene            2639..>2916
                     /locus_tag="AC04VQ_06890"
     CDS             2639..>2916
                     /locus_tag="AC04VQ_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024252.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIO9553825.1"
                     /translation="MTYFSNNIKFLRKHRNRTQDDVAFALNMKRSTLSGYENEVSEPN
                     IEALVAFSKYFGIAIDTLVKVDLSTISESQLLQLERGYDVHLKGSNLRV"
BASE COUNT         1046 a          568 c          513 g          789 t
ORIGIN      
        1 tgcgtttcac aaaaaagttg tatttttgac gagtttttaa tcaaaatcaa cgacgcaatt
       61 ttaatcattt atttttaaac agaacagata ttttttttga ttttttgact ttgagaatga
      121 caatttgaac aatttaatga tagacaatag aatataatta tcattacaga acaataaaac
      181 aaacatggca cctaacaaca acccaacagc agaaatgcaa aatagtaatt tttcgctata
      241 tgacttctgc aaaagacttt ccaaaaacat cactggtgtt tatgacaaat cttgtttaga
      301 tgatgacgga catattattt catttaacga gcaagccgct attatcacac attatttgca
      361 ccaacaatcc gacaatatcc aacttgagaa caaagccacg gaattactaa gactatggta
      421 tagcttcttt gaaagggaca aaaaagattc tagcatggtc catgataatt cagatgcatt
      481 tactggctac ctatttagcg aatgcttcaa tgtcccattt agagcttcac taaagccaaa
      541 attcaagttt attgatttat ttgctggcat tggcggattc cgaatcgcta tgcaaaatct
      601 cggaggagaa tgtgtatttt cttcagaatg ggatgaacaa gcccaaaaaa catattacac
      661 aaattttggc gaagtaccat ttggagatat tactaaagaa tcaacaaaaa aatatattcc
      721 gcaagacttc gacatccttt gcgctggctt cccttgccaa gctttctcgc tagctggtaa
      781 aagacgagga ttcgaagaaa caagaggcac actttttttt gatgttgccg aaatactaaa
      841 aaaacatcaa ccaaaagctt ttttcttaga gaatgtaaaa ggattaatct cacacgacaa
      901 aggcaagacc cttcaagtaa ttctcaacac acttgacgaa gttggataca ttgttcctga
      961 ccccaaaatc atcaacgcta tgtactttgg cgttccacag catagagaaa gaatatatat
     1021 agtaggattt agaaaagatt tagggataaa aaaagaagat ttttcatatc cagagcaaaa
     1081 agaaatatca aaacattgga ttgatgtacg ggaagaaaat cctgttcctg caaaatacta
     1141 tttatcaacc caatacatac aaaccctcat caaccataaa gcgaggcacg aagccaaggg
     1201 acacgggttc ggttacgaca tcatttcaga tgacggggtt tcccacgcta tagtggttgg
     1261 aggcatgggc agagaaacaa atttagtaat tgactttagg caaaccgact tgacaccaac
     1321 aacaagaata aaaggcgaag tcaataaaca aggctggagg aaaatgactc ctagagaatg
     1381 ggccagattg caaggtttcc ccgatgaatt tgtcatcccc gttgcagacg cttccgcata
     1441 taagcaattc gggaacagcg tcgcagtccc tgccattcaa gccaccgcag aacaactcat
     1501 caaaacttta gaaaggaatt aaaatcatgg ctttatcagg aaacaaagga gaatggagcg
     1561 agatatatgc tttatttaaa cttttaagcg aagggaaagt tcatgcaggt gatgctgaaa
     1621 tgaataagat ggatttattt tatcctataa tagacataat tcgcgaagag agcaagcgat
     1681 acgaatacct tccgaatgta aatcaaaaca taataataat ccatgaaaac ggagaagaat
     1741 atggaagaat ttccatggat aagttcttct tagaatcaaa aaatttgctg aacgttttaa
     1801 gcaaagcgaa tgatagagct tttgaaatac catcaactga actctttatg aatcaaattg
     1861 gatgctcaaa actcaaagca ccatcaaaag acaaagcaga cattcatatt atcattcatg
     1921 atttacacac aaatatgcaa cctttattag gttttagcat taaatcacaa ttaggcagtg
     1981 cttctactct attaaacgct ggcattacaa caaattttac ctttaaaatt acaggtaagg
     2041 atctatctga caacgatata gccaacatca acaacattta cgaacattta cctagaatgg
     2101 aaagcatcat aaaacacgga tgcaaattag aattcgttga cgttgaacac cagactttca
     2161 aaaacaacct tctctttctt gattgttgta tgcctgaatt tttagctcta tgtctattag
     2221 cagacagtat gcctaattca acatcttcaa taaaagacat tgtcaaaatt gtttctacaa
     2281 ccaatccttt tcatttcact gggaatgaca ttgaatcgtt ttatgagcac aaaatgaaag
     2341 tgcttttaat agatgcggct cttggcatga cacctgcaaa agaatggaaa ggaaagtacg
     2401 acgcaaacgg tggatacctt gttgtaaaaa acgatggaga aatcgtttgt tatcattttt
     2461 acaacagaaa cgatatcgaa gactatttat tcaacaacac ccgttttgaa agagcaagcc
     2521 gaagcagata tcactttgga acattatatc gaggaactga caattgtgtt tacttgaaac
     2581 tcaatcttca gattagattt aaaaaataat tctataaccc caaatttcga gcctaacaat
     2641 gacatatttt agcaacaata tcaaattcct ccgcaagcac cgcaaccgca cacaagacga
     2701 tgtggccttt gcgctcaaca tgaaacgctc cactttgagc ggctacgaaa acgaagtttc
     2761 ggagcctaac atcgaggctt tggtcgcctt ctcgaaatat ttcggcatcg ccatcgacac
     2821 tttggtgaaa gtcgatttga gcaccatcag cgagagccaa ctgcttcaac tcgaacgcgg
     2881 ctacgacgtg catctgaaag gcagcaacct gcgcgt
//
LOCUS       DBNCDC010000110         2828 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_28889_length_2828_cov_6.256401, whole genome shotgun sequence.
ACCESSION   DBNCDC010000110 DBNCDC010000000
VERSION     DBNCDC010000110.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28889_length_2828_cov_6.256401"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(60..683)
                     /locus_tag="AC04VQ_06895"
     CDS             complement(60..683)
                     /locus_tag="AC04VQ_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIO9553826.1"
                     /translation="MSSLFGPGVMILFFIFVAALIFLYVMSILWAKKDAKARGANEKS
                     AMLISLIPFAGVLAYVLMRPPLLKIDEEEQNLDIKLKTRQLKKYDVCPSCGAEIEDSY
                     VYCPKCNKKLKEICTNCNKPLDLSWNSCPYCGTLANKSASDSYYTKQENIEAPYNEEA
                     KKDEQPQVDYKVEDSFSFDENAVEDITPDNIAQPKHHFKNHNNKTHN"
     gene            753..1331
                     /locus_tag="AC04VQ_06900"
     CDS             753..1331
                     /locus_tag="AC04VQ_06900"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIO9553827.1"
                     /translation="MNFSKIILLSDTHMRLGENVQMAVCGDYSDDQIYERQFFTKDED
                     LVNNFLSQKDELDCQKPNLIIHAGDVGNQEIIDILESIAPVKVVNGNCDFQSFRTIEG
                     KSGDYEYFDFEGIKISLAHSPYDLDFGDDNREVNMHVHGHTHESYITQLGKNEIKICP
                     GSATMGRYGTPNSIACVYITDKKLISAELIKV"
     gene            1381..1539
                     /locus_tag="AC04VQ_06905"
     CDS             1381..1539
                     /locus_tag="AC04VQ_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553828.1"
                     /translation="MPKLAKGHEEEQKFNAIVMDLMRKSPTSFHVVKNNMDILNDNGF
                     VRLRENDT"
     gene            1543..2721
                     /locus_tag="AC04VQ_06910"
     CDS             1543..2721
                     /locus_tag="AC04VQ_06910"
                     /inference="COORDINATES: protein motif:HMM:NF014213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding metallopeptidase family protein"
                     /protein_id="HIO9553829.1"
                     /translation="MKPGGKYYMTRNNSAIIAFKIPNAKKFNGFMVGASHSDHPCFKI
                     INDCEVNVEDHYKIVTIEQYPKAIDYTWFDTPLSVAGRLIVKTKDGFETKLVDVNRPL
                     LMIPSLASHMQKEYDSIEPLFECETRMRPIFSTNKDITLMDIVLGENNIDPKQVIDSE
                     LFVYQHRDPVIWGADNEFMSSRAIDDQLSVYSSLMAFIDSDVKESIAMHAVFDNEEIG
                     SYTTQGADSTLLANVMMRALSSMKMDRVDFRAMLPNSFILSIDNAHGLHPNYVEKYNK
                     IARTYLGKGAVLKKAASYSYCTNAVSSSLLHRMAEKEGAKLQDYYNRPGVIPGSTLAR
                     YLLPYTSVIGADVGIAQLAMHSGYETCSAYDALHLKKLIQSVYSSTLIMHDDGNYTVK
                     "
BASE COUNT          921 a          500 c          533 g          874 t
ORIGIN      
        1 atatatcctc cctaatacat agttgaacaa gcgcacaaag ccagtcaata tagataaagc
       61 taattgtgtg ttttgttgtt atgattttta aagtgatgtt ttggttgagc aatattatct
      121 ggagtaatat cctcaacagc attttcgtca aagctaaaag agtcttcaac tttatagtca
      181 acctgaggct gctcatcttt ttttgcctct tcattgtaag gagcttcaat gttttcttgc
      241 tttgtataat aactatctga agcgctctta tttgccaaag tgccacaata tgggcatgaa
      301 ttccaactta gatcaagagg cttattgcaa tttgtgcaaa tctcttttaa ttttttattg
      361 cattttggac aataaacata actgtcttca atttcagcac cacaacttgg gcatacgtca
      421 tattttttta gctgcctggt tttaagctta atatctagat tttgctcttc ttcgtcaatt
      481 ttaagaagag gaggtctcat aagaacgtat gctaaaactc cagcaaatgg aatcaacgaa
      541 atcaacatgg cagacttctc gtttgctccc cttgcttttg cgtctttttt tgcccataaa
      601 atggacataa cgtataaaaa tatcagggca gcaacgaata taaagaataa aatcattacg
      661 cctggtccaa ataagctgct catttcatcg cctttcttaa atcgtcaagc agatattcta
      721 ccataaatga taaattttga tataatcaag ctatgaattt ttcgaaaatt atattattaa
      781 gcgataccca catgcgtttg ggagaaaacg tccaaatggc cgtttgtgga gattattcag
      841 atgatcaaat ttatgagcgg caatttttta caaaagacga agaccttgtc aataatttcc
      901 taagtcaaaa ggatgagctt gattgtcaaa aacctaattt aattatccat gctggagatg
      961 tcgggaacca agaaattatt gacatcttag agagcattgc gccagttaaa gtggttaatg
     1021 gtaactgtga ttttcagagt tttagaacaa ttgaggggaa aagcggagat tatgaatact
     1081 tcgactttga aggtataaaa attagcctcg ctcattcacc ttatgatcta gattttggcg
     1141 atgacaatcg tgaagtaaat atgcatgtac atgggcacac tcacgaatct tatatcacac
     1201 aattaggcaa aaacgaaatt aaaatctgtc ctggttcggc aactatggga agatatggca
     1261 cgccgaatag catcgcttgt gtgtatatta cagataaaaa actcatatca gcagaactta
     1321 tcaaagtgta gacacaaggt tttatgttaa aataaaaagc ccccaataaa aggagaaaaa
     1381 atgccaaaac tagctaaagg tcacgaagaa gaacaaaaat ttaacgctat tgttatggac
     1441 ttaatgcgca agagtccaac aagttttcat gttgtaaaaa acaatatgga tatattaaac
     1501 gataacggat ttgtgcgtct tcgtgaaaac gacacatgag aaataaagcc tggtggaaag
     1561 tactatatga ctcgtaacaa ttccgctatt attgcattta aaattccaaa tgcaaagaaa
     1621 ttcaatggtt ttatggtggg agcaagccat agtgatcatc catgttttaa aattatcaac
     1681 gattgcgaag ttaatgtcga agaccactac aagattgtaa caatcgaaca gtatccaaaa
     1741 gctattgatt acacatggtt tgacactcct ctatctgtag ctggccgttt aattgtaaaa
     1801 acaaaagatg gtttcgaaac aaaacttgta gatgttaaca ggcctcttct tatgattcca
     1861 agtttagctt ctcatatgca aaaagagtat gactctattg agcctctctt tgaatgcgaa
     1921 acaagaatgc gaccaatttt ctcaacaaac aaggacatca ccttaatgga tattgtgttg
     1981 ggcgaaaata atattgatcc taagcaagta attgatagcg aactttttgt ctatcaacat
     2041 agagatcctg ttatttgggg tgctgataac gaatttatga gttcacgcgc tattgacgat
     2101 caactttctg tttacagctc actaatggca tttattgata gtgatgtaaa agaaagtatt
     2161 gcaatgcatg cggtatttga taacgaagag atcggttcgt acactactca aggagcggac
     2221 tctaccctgc ttgcaaacgt aatgatgcgt gccttgtctt cgatgaaaat ggacagggtt
     2281 gactttaggg ctatgcttcc aaattctttc atattgtcaa tcgacaacgc acatgggctt
     2341 catccaaact atgtcgaaaa atataacaag attgcaagaa cttatcttgg aaaaggtgct
     2401 gttttaaaga aggctgcttc gtattcgtat tgcacaaacg cggtttcttc ttcgcttctt
     2461 cataggatgg cggaaaaaga aggtgcaaaa ctccaagatt attataatag acctggtgtt
     2521 attcctggtt ctacacttgc tcgttatctg ttaccataca catctgtaat tggagccgat
     2581 gttggtatcg cccaacttgc aatgcattct ggttatgaaa cttgcagcgc ttatgacgct
     2641 cttcatttaa agaaattaat acagtcagtt tattcttcga ctttgattat gcatgacgac
     2701 ggaaactata cagttaagta agggggaaaa atgagtctaa tttcaccaga tttagttatt
     2761 tttgatagcg gcgtttctta tagagacgaa gccatcaagc aaatgtctga cgctatggaa
     2821 gccgcagg
//
LOCUS       DBNCDC010000111         2778 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_29850_length_2778_cov_7.053250, whole genome shotgun sequence.
ACCESSION   DBNCDC010000111 DBNCDC010000000
VERSION     DBNCDC010000111.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29850_length_2778_cov_7.053250"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            124..657
                     /locus_tag="AC04VQ_06915"
     CDS             124..657
                     /locus_tag="AC04VQ_06915"
                     /inference="COORDINATES: protein motif:HMM:NF015573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HIO9553830.1"
                     /translation="MGINNLVMSLTGSETSTITLPDYIGAMFVSVLVRNLNEKFHWFN
                     YNAKFCDGLGGITLDVFLSIAMMSIDLLTLIELGPRLAVIVLAEVVIMGLYVYFIVFR
                     ALGKNFDAAVMCGGMCGHALGATPTAMANMEAVSARFGFSQKAFVIVPIVGAFLVDLV
                     YQPVSIVLINMFVPYVA"
     gene            743..961
                     /locus_tag="AC04VQ_06920"
     CDS             743..961
                     /locus_tag="AC04VQ_06920"
                     /inference="COORDINATES: protein motif:HMM:NF049449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIO9553831.1"
                     /translation="MEEKLINIISEQTDVPREEVTLDSSLVELGIDSMSMVVLVSVIE
                     ASFNVKLNTAKIANVYTVGDLIPLLKHE"
     gene            954..1361
                     /locus_tag="AC04VQ_06925"
     CDS             954..1361
                     /locus_tag="AC04VQ_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIO9553832.1"
                     /translation="MNKKLQINCAKGVAIIIIVLSHLMLANTYTPVSFAPLLGFCVPM
                     FFYCTGYEYVVYRGTLGKELRVRVLRLLMYALGVFLLFVAIQSIILYFYDGFDFVSCL
                     KSLFLLVTDESALTFFPEGSRVSYDYKNILCPY"
     gene            1518..1727
                     /locus_tag="AC04VQ_06930"
     CDS             1518..1727
                     /locus_tag="AC04VQ_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553833.1"
                     /translation="MAPSFTFVMLAAYLIKEFKIEQMLEKLNFPTQLIINLAIFIIGE
                     IIASQIGLEKIARGRFAENDASFLC"
     gene            2114..2596
                     /locus_tag="AC04VQ_06935"
                     /pseudo
     CDS             2114..2596
                     /locus_tag="AC04VQ_06935"
                     /inference="COORDINATES: protein motif:HMM:NF019454.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2639..>2778
                     /locus_tag="AC04VQ_06940"
     CDS             2639..>2778
                     /locus_tag="AC04VQ_06940"
                     /inference="COORDINATES: protein motif:HMM:NF014965.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553834.1"
                     /translation="MAIGARKCLVAFNVNLKTDDVEIAKKIASEIRESNGGFKEVKAM
                     GF"
BASE COUNT          793 a          417 c          554 g         1014 t
ORIGIN      
        1 ccaaagaaag ctgctccaaa aactcgtgaa gaacgtcgtt caagtgctat ggatattatg
       61 aaaaatttcg cagttattgt aatttgtatg acagctggta ttttggttgc taaatgagca
      121 tcaatgggaa tcaacaattt ggttatgagc ttaactggtt ctgaaacttc gacaattaca
      181 ttgcctgact acattggtgc tatgtttgtc tctgttttgg ttcgaaactt aaatgaaaag
      241 ttccactggt ttaactataa tgcgaagttc tgtgatggac ttggcggtat tactcttgat
      301 gtattcttga gtattgctat gatgtcgatt gatcttctta cattgattga acttggtcca
      361 agacttgctg ttatcgtttt ggccgaagtt gttattatgg gcttgtatgt atactttatt
      421 gtgttccgcg ctttgggcaa aaacttcgat gctgctgtta tgtgcggtgg tatgtgcggt
      481 cacgcattgg gtgcaacgcc tacagctatg gctaacatgg aagctgtttc tgctagattc
      541 ggtttcagcc agaaggcttt tgttattgtt cctatcgtag gtgcgttctt agttgatctg
      601 gtttatcagc cggtaagtat tgtacttatt aatatgtttg tgccgtacgt tgcttaggct
      661 aagtttttga tagaattttt gaaaagcagt catttcggct gcttttcttt tataatatac
      721 actttgtttt gcgactaaaa atatggaaga aaagttaata aatataatta gcgagcaaac
      781 agacgtacca agagaagaag taacgcttga ttcttctttg gtagaattag gcatagattc
      841 tatgtctatg gttgttcttg tttctgttat tgaagcttcg tttaatgtaa aacttaatac
      901 tgctaaaatt gcaaatgtat atactgttgg tgatttaatt cctctactaa agcatgaata
      961 agaaactcca gataaattgt gcaaaaggag ttgcaattat tataattgtc ctgtctcatc
     1021 tcatgcttgc gaacacatat actccagtat cttttgctcc tttacttggt ttttgtgttc
     1081 ctatgttttt ttattgcact ggttatgaat atgttgttta ccgaggtact cttggaaaag
     1141 aattaagagt aagagttctt aggttattaa tgtatgcact tggtgtattt ttattgtttg
     1201 ttgcaattca gtctataata ctgtattttt acgatgggtt tgattttgtt tcttgtttaa
     1261 aatcattatt tcttttagtt actgacgaaa gtgctttaac attttttcct gaaggtagtc
     1321 gcgtttcgta tgactacaaa aacatacttt gtccatactg atttatcttt ttatttgttt
     1381 taactttact tatttttatt gtcgtagtta gaattcttcg aaaaaataaa tatgcaggaa
     1441 tagctgccac attaatactc ttgattgtaa gtgctcttat atattgtttt gcaggagttt
     1501 ttcctgctgc ttctcatttg gctccttctt ttacatttgt tatgctagca gcttacctta
     1561 ttaaggaatt taagatagag caaatgcttg agaagctaaa ttttcctaca caactaataa
     1621 ttaatttagc tatctttatt ataggcgaaa ttattgcttc acaaattgga ttagaaaaga
     1681 ttgctcgtgg gagatttgct gagaacgatg caagtttttt gtgctgattg atgggtatga
     1741 tggcaatttt actttgttca tttagttttg ttaatttatc tataattttt actaaaatca
     1801 aaggtgtttc tcaagtattg tcttgaggag gcgttaatgc tcttgatatt ttacttgtac
     1861 atatgttttt tgcaacagag ttctcgaaat ttacaggcat ccctaatgga aggtgatttt
     1921 ttgattcttc gcagattaca ccacaattgc aaattcaatt ctttttagta ttagttgttt
     1981 ccatttcact cactgctggt tatatttatc taaaaaaata tttctttaga aaacgagcat
     2041 taaatagatt atcaaaataa ttttttctcc taagttttgg tataatcgtt tcgtcaagtt
     2101 ttaaggagat attatgaaaa aaattatcga aacaattcca aattttagtg tatgtaaaga
     2161 aaatgacgaa gccacattta acgggcttgt agatatttca aagagccgaa gcgatgtaat
     2221 cttgcttgac gctcaaagcg atgttgatca caaccgaagt gtttttactc ttattgggga
     2281 gccagaaggt gtagaagaaa ttgccttcca acttactaaa tttgcaggtg agcatattga
     2341 tatgaatcat caccatggtg agcattcgcg tatgggcgca actgatgttt tgccttttgt
     2401 tccaacgcta aacgttgaag ttgaagaatg cattgaaatg agcaagcgag ttgcaaagcg
     2461 catttgagat gaacttcaaa taccttcgtt tttatacgaa gaatctgcaa ccaagcctga
     2521 acgtaaaaat ttggcaacag ttcgaaaagg tcagtgagaa ggcatgcctg aaaaattgca
     2581 acaagaagag tgggcaccgg acttcggaga gagaaagatg catccaacag ctggaattat
     2641 ggccattggc gcacgtaaat gtctagttgc cttcaacgta aatcttaaaa ctgatgatgt
     2701 tgaaattgct aaaaagattg caagtgaaat tcgcgagagc aatggaggct ttaaggaagt
     2761 taaagctatg ggctttag
//
LOCUS       DBNCDC010000112         2678 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_31894_length_2678_cov_7.174990, whole genome shotgun sequence.
ACCESSION   DBNCDC010000112 DBNCDC010000000
VERSION     DBNCDC010000112.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31894_length_2678_cov_7.174990"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(206..724)
                     /locus_tag="AC04VQ_06945"
     CDS             complement(206..724)
                     /locus_tag="AC04VQ_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553835.1"
                     /translation="MAIVDLLDNCQIEDFPPFKETFIQNGMNCEICDLRDLRFDGENL
                     TNKDGYKINAVYKRITLDDLIPFKDSEGAQAFVEAVFNDKVCPIDWFNSQLVHDKQLF
                     AILHEPETQKLLNESEKDFVKLHVPETHILSNNIKNIKKFIDNKDDYLIKPINSRDAK
                     NVFIGKEMNEKD"
     gene            complement(1556..2542)
                     /locus_tag="AC04VQ_06950"
                     /pseudo
     CDS             complement(1556..2542)
                     /locus_tag="AC04VQ_06950"
                     /inference="COORDINATES: protein motif:HMM:NF016032.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
     gene            2588..2678
                     /locus_tag="AC04VQ_06955"
     tRNA            2588..2678
                     /locus_tag="AC04VQ_06955"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2622..2624,aa:Ser,seq:gct)
BASE COUNT          754 a          494 c          447 g          983 t
ORIGIN      
        1 ctagccttct ttggcgtaaa tacgttccac taaaatgtga attatagcag tatagccctt
       61 ccatattaca gcattgttga atactgctgg tcaaggtatc atttttatta tagatatcaa
      121 cacaaatatt tgcgcttgtt tgcattgttg caaatttttg aataacgaat ccatcgatct
      181 ctaaacattt tttaagagtt gcttctcaat ctttttcatt catttctttg ccaataaaaa
      241 catttttggc gtctcgtgaa tttataggct taattaagta atcatctttg ttgtcaataa
      301 atttcttaat gtttttaata ttgttgctta atatatgagt ttctggaacg tgcaatttca
      361 caaaatcttt ctcagattca tttagcaatt tttgagtttc tggctcatga agaatcgcaa
      421 ataattgttt gtcatgaacc agctgactat taaaccaatc aatagggcat actttgtcat
      481 tgaagacagc ttcaacaaag gcttgcgcgc cttcgctgtc tttaaatgga attaaatcat
      541 ctagcgtaat tcgcttataa acagcattaa ttttgtagcc gtctttgttt gtcaagtttt
      601 caccatcgaa gcgaaggtcc cgcaagtcgc aaatctcaca attcattcca ttttgaataa
      661 atgtttcttt aaacggaggg aagtcttcaa tttgacagtt gtccaacaaa tcaacaattg
      721 ccacattgat tttcttgctt tcgtgtttag tattttttgc ccattcaaga tagatctttt
      781 caaattcttt tactcaaggg gtgtagacat cagcgctatt gaaattggcc tttcctttag
      841 tctcctcaat tactttttta taagggacgg tgtttttgat ttcttcgcaa gcacaggtta
      901 ctttattcat tgcagcgctt ccgcctgtat tgagttcgca aaattcgaat tgcccaattg
      961 catcgttaaa gaaaatatca aatcttgcaa cagggaagtc tgccgcgtat tttggtttaa
     1021 agtttataag cttcgatgaa tattcatccc attcaaaaag ggatttgaac tgcgagtttt
     1081 tatagtattc atcatatatt tttttgagaa tattgcatgt cttttgcgtt agtaatttaa
     1141 attgaacttg gttatttatg tcaaaaaact tagcgaagta acctgtggtt gatactttgc
     1201 cttcgtaggc aacttttgag tagtgtctaa atttaagatg atcttctaag tcttgcttgc
     1261 aatctttaaa tgcattagct tgaatttgag tccacgtttc agttttgcta actggagaaa
     1321 atgatccctc aatttccggg taatcatttt ttatgccatc aagaagacaa cttttattct
     1381 ctactagctt ttcaatcgga cttaagtaat tgcatggctt ggtttttgcg agatctaata
     1441 aatctagtca agcatcataa atgttttgtc cataaacatt tgcatttcat cctttttctt
     1501 gaagctcttt tcccgcattt ttataatcct caaaagttaa attcttatat ttatctcaaa
     1561 gtttgtctaa ggcttctgaa ttgtaaaaga tgttttgaag caattcgata tatgccattg
     1621 tgaaatcttt tggcatgcta tccgcaggac gaatttcaat tttattttta agtctggcat
     1681 cataaaaaca catagaaagt tcgtgcttaa ttttctcttc gtcattttcg aagttttttg
     1741 gcatataaac ttcaggcaaa gcttttataa catccgcgta tttttcatat gagaaatcat
     1801 catcaaaaat ttcaggcacc gaagcgcacc tgtctgcgtc ggcattgttt catatttgcc
     1861 atcttgccat acgttttgca atagggatgc cacttgggct taaaccttcg tttccgactt
     1921 tacatctctc aaaaataggt gagttatcaa gcaaaaaata aattagtggg cttattgccg
     1981 ttgcaacttt catttttttc ttgcaatctt cttcgcttga atagtcaatt gtgagttgag
     2041 ttgcggcaga acatcgcatc atgcaaaaac cttgagttcc aacattttta aaattagcat
     2101 ccatgcaaat gtatcttagt tttggaataa taggaacttc caaagcgcag caatcaggtc
     2161 tgtaacctat atgagctaag tagtatttgt gcttgttaag aacaactttt acttcttcca
     2221 gaaacttatt gtaaatgttt tcaatttctt ggacagtttt gcactttttg attgaaactt
     2281 caaattgcgc acctggctct atagttatgt cacaaccatc tctgcgaagc ccaatcaaat
     2341 attgttcacc attgaagttt tcaaagattt tttcattata gttaggcgac aactcttcaa
     2401 taatgtcttg aacgccaggt ttgttgtctt cgtacgaata aaaaacggat ttattatttt
     2461 catctaaaat aaaatgctca atttcggcgc ccaaaaactg ttcagaagtt tcaccatttt
     2521 ttatgtattc agataattgc atacttgtat tatattgtaa tatgctatta tacgggcttg
     2581 caattgtgga gagctgaccg agaggccgat ggtgctcgcc tgctaagcga gtgtacccca
     2641 caagggtatc cggggttcga atccccggct ctccgcca
//
LOCUS       DBNCDC010000113         2673 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_32002_length_2673_cov_10.048128, whole genome shotgun
            sequence.
ACCESSION   DBNCDC010000113 DBNCDC010000000
VERSION     DBNCDC010000113.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32002_length_2673_cov_10.048128"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..79)
                     /locus_tag="AC04VQ_06960"
     CDS             complement(<1..79)
                     /locus_tag="AC04VQ_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024246.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIO9553836.1"
                     /translation="MAAPTVNADECTACGACVDACPCGVL"
     gene            complement(142..1353)
                     /locus_tag="AC04VQ_06965"
     CDS             complement(142..1353)
                     /locus_tag="AC04VQ_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIO9553837.1"
                     /translation="MALNVKVGLIGVMDMLKFKNMSKTREAAIQKAPANEIQTEYEIN
                     QSAKALHPDFQTLEVVDIVDYKDAGAKKYIFKAAEGKLPNFRAGQYLSFKLHGDDFFT
                     TRPYSICSTPNDAVDNGKVAVTIKTNPGGFIADLILKHMKVGDKVKASAPEGQFYYES
                     LRDAKDVIALAGGSGITPFLSMAGAIAEGTEDFNLTILFGSRTEDAILFKDELAEIEK
                     ASNGKVKVVHILSDEKKDGYENGFISADIIKKYAPKGDYSIFICGPEAMYKFAEKEVA
                     KLKLPRRRVRNELLGVTKDVTTDPKYKGKADKTFNVTVKQAGQEPRKIKAAANETLLV
                     AFERAGIKAPSRCRSGECGWCRSQLVSGDVFVPEATDGRRFSDITWNTIHPCATFPLS
                     DCEVEVPGEYL"
     gene            complement(1355..1591)
                     /locus_tag="AC04VQ_06970"
     CDS             complement(1355..1591)
                     /locus_tag="AC04VQ_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553838.1"
                     /translation="MGNAEGNVYGYENRDWDTLVARMQMIETDYPIKGLRPIGACGPR
                     GDGYNSAYVTGQMMAGLALRDMAQWAAQGSKGGE"
     gene            complement(1607..>2673)
                     /locus_tag="AC04VQ_06975"
     CDS             complement(1607..>2673)
                     /locus_tag="AC04VQ_06975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIO9553839.1"
                     /translation="DWIEIPDLFRVVSTWTDDGTEMDVTMPTGVEPFIKKMEEYVPGS
                     EKSMRDFFDLCIEVVDANEYIEEANGNPDSNVLREKYPNYLRTGAYSVTKVFKALKMP
                     QRSQDILATYWGYLGVDLPHLNFVHYAAMIYTYVIFGPHIPTHTSHEISSACIARLRE
                     LGGDVWYNCRAEKFLFDGDKLCGVETSLGTVECDYALPNINPNFVYGKMIPKELIPER
                     EKKLSTARGNKFSGRFIGAYFCLDKTIEELGIKDYTIFMSETSDSEKSYNNIYNGSDI
                     SQHSTFVCYNVANKNFSPKGTTVCSFTTLGNPKVWEGLSQKEYFKKKEAFAKQCIELL
                     KRKTGIDISDCIEEMSVATP"
BASE COUNT          777 a          622 c          482 g          792 t
ORIGIN      
        1 ccaaaacacc gcatggacat gcatctacgc atgcgccaca agctgtgcat tcatctgcat
       61 taactgttgg tgctgccata ataatctcct tttctcagaa tttgcgccct tattgacgca
      121 aaaaacaatt ttatcacaaa actataagta ttcgcctggg acttcaactt cacaatcact
      181 taatgggaaa gttgcacaag ggtgaattgt gttccaagtg atgtccgaaa agcgacgtcc
      241 atctgttgct tcggggacaa atacatcgcc agatactagc tgagatctgc accagccgca
      301 ttcaccgctt cggcagcgtg aaggtgcttt aattccagcg cgttcaaaag caacaagcaa
      361 agtttcattt gctgcagctt taattttacg aggttcttga cccgcttgct taactgttac
      421 attaaatgtt ttgtctgcct tgcctttgta ttttggatca gttgtaacat cttttgtaac
      481 acccaaaagc tcatttctaa cacggcgacg cggaagcttt agcttcgcta cttccttttc
      541 tgcgaactta tacatagctt caggaccgca aataaagata ctgtaatcgc ccttaggagc
      601 atacttttta ataatgtctg cactaataaa gccgttctca taaccatctt tcttttcatc
      661 agaaagaatg tgaacaacct taacctttcc atttgaagct ttttctattt cagcaagttc
      721 atccttaaac aaaattgcat cttcggttct gctaccaaaa agaattgtta gattgaaatc
      781 ttcagttcct tcggcaatag caccagccat gctcaagaaa ggagtgatac cacttccacc
      841 ggcaagcgca ataacatctt tagcatcgcg aaggctttca taataaaatt ggccttcagg
      901 agccgaagcc ttaaccttat ctccaacttt catatgctta agaattagat cagcaatgaa
      961 tccgccagga tttgtcttaa tagttacggc aactttgcca ttatcaaccg catcgtttgg
     1021 tgtagagcaa attgaataag gacgtgttgt gaagaaatca tcgccatgaa gtttaaaaga
     1081 caaatattgg ccagcgcgga agtttggaag cttgccttcg gctgccttga aaatatattt
     1141 tttggcgcca gcgtctttat aatcaacaat atcaacaact tccaaagttt gaaagtccgg
     1201 atgcaatgcc ttcgcacttt gattaatttc atattctgtt tggatttcgt tcgcaggagc
     1261 tttttgaatt gcagcttcac gagtcttgct catattctta aatttgagca tatccataac
     1321 accaattaaa ccaactttaa cgttcaaagc cattttactc accgcctttc gaaccttgag
     1381 ctgcccactg tgccatatca cgaagcgcaa gaccagccat catttgacct gttacatagg
     1441 cagaattata tccatcaccg cgaggtccac aagcgccaat tggacgcaga cccttaattg
     1501 gataatcggt ctcaatcatt tgcatgcgag caaccaaagt atcccaatca cggttttcat
     1561 aaccgtaaac gttaccttcg gcgttaccca tatatcttgc aaaagttcaa ggagttgcaa
     1621 ctgacatctc ttcgatgcaa tcgctaatat caattccagt cttacgtttg agcaattcaa
     1681 tacattgctt tgcgaaagct tctttcttct tgaagtattc cttttgactc aagccttccc
     1741 aaacttttgg attacctaaa gttgtgaaag aacaaacggt cgttcccttc ggagagaaat
     1801 tcttgttagc aacgttatag caaacgaacg tcgaatgctg actgatgtcg ctgccattat
     1861 aaatattgtt ataagatttc tcagaatcag aagtctcgct cataaagatt gtgtaatcct
     1921 taattcccag ctcttcaata gttttatcga ggcagaaata tgcaccgatg aatcgaccac
     1981 tgaatttatt tccacgagct gttgaaagct tcttctcgcg ctctggaatt aattcttttg
     2041 gaatcatctt tccataaaca aaatttggat taatgtttgg aagcgcgtaa tcgcactcga
     2101 cagttccaag cgaagtttca acgccacaaa gtttgtcgcc atcgaacaag aatttttctg
     2161 cacgacagtt ataccaaaca tcgccaccaa gctcacgaag acgagcaata caagccgaag
     2221 aaatttcgtg agatgtgtgc gttggaatgt gtggtccaaa aattacatag gtgtaaatca
     2281 tcgcagcata atgaacgaaa tttaggtgcg gcaaatcaac accaagatat ccccaatatg
     2341 ttgcaaggat gtcttggctt cgctgaggca tcttcaaagc cttaaaaact tttgtaactg
     2401 aataagcacc cgtccttaaa tagttaggat acttttcgcg aagaacattt gagtccggat
     2461 tgccgtttgc ctcttcgatg tattcattcg cgtccactac ttcaatacaa agatcgaaga
     2521 aatccctcat gcttttctcg ctgcctggaa cgtattcctc catctttttg atgaaaggct
     2581 caacaccggt tggcattgtg acatccatct cagttccatc atctgtccaa gttgaaacaa
     2641 cgcggaacaa atctggaatt tcaatccaat caa
//
LOCUS       DBNCDC010000114         2632 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_32871_length_2632_cov_6.377959, whole genome shotgun sequence.
ACCESSION   DBNCDC010000114 DBNCDC010000000
VERSION     DBNCDC010000114.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32871_length_2632_cov_6.377959"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(1..700)
                     /locus_tag="AC04VQ_06980"
                     /pseudo
     CDS             complement(1..700)
                     /locus_tag="AC04VQ_06980"
                     /inference="COORDINATES: protein motif:HMM:NF014801.7"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
     gene            complement(806..1411)
                     /locus_tag="AC04VQ_06985"
                     /pseudo
     CDS             complement(806..1411)
                     /locus_tag="AC04VQ_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012589.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
     gene            1863..2084
                     /locus_tag="AC04VQ_06990"
     CDS             1863..2084
                     /locus_tag="AC04VQ_06990"
                     /inference="COORDINATES: protein motif:HMM:NF024740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553840.1"
                     /translation="MRKIEADPDRDYIFFTNNASKVPSIYVEKLAKLGLDVDESKIVT
                     AGDVCAQFLKENYPGAKIFLNGTPALEEN"
     gene            2253..2543
                     /locus_tag="AC04VQ_06995"
     CDS             2253..2543
                     /locus_tag="AC04VQ_06995"
                     /inference="COORDINATES: protein motif:HMM:NF024639.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HIO9553841.1"
                     /translation="MPDCGAMCELIKSSTGVAPRFFGKPYKETVDMLTSITGIEAKHM
                     AFVGDRLYTDVATGVNNGAVGILVLSGESTIQTVEESDVKPTAIYNSICEIS"
BASE COUNT          780 a          574 c          518 g          760 t
ORIGIN      
        1 aagtttccgc cttgtgaaca agcgcattcg cggctttttc ataatctcca ttgtagagat
       61 aagatgcagc accaacaaca cagcaaccaa gaacacaacc ttgatcgcat tcattaacaa
      121 caattggctt gccacaaata tcagcaatca tttgaaccca agcaatattt ttggttccgc
      181 caccacaagc tacaatctct tcaatgtcga aggcctcttc ttcgtacttc gtaataacat
      241 tgtaagttcc aaaagcaaca ccttcaagga ccgcacgata catatccttt cagctatgtt
      301 gcatggtcaa accataaaag gcgcccttcg aaaaaggatc cttgtaagga gttctgttgc
      361 cctggaagtg gtcgagcata ataactccac cagcaccagg ttcggtttct cgcagcgctt
      421 cagccaaata aacgtaaaca tcaatttcgc aaatttgata aatgtggaaa ttgcgaataa
      481 accaatttac aagacctgca gccgaggact gcccgccttc gatacaaccc attccgtcga
      541 caaccgcatt tttataagga ccccaaaaac tgttaagctt cttggaactg tcattgatgt
      601 tgccaaggat gcaaaaactt gtgcccatat tgatcgaaag cttgccttcg gtaaacgcgt
      661 tagttccaaa cattccaatg tgagcgtcga tgccgcccat gataagcttg gcgtccttcg
      721 aaatgccgaa tttctcgcaa atctcaggtt ttaaaggacc aaggtccgca ccaataagct
      781 cgatgcgttc gaaaaccttg tcatttcaat cttccaaacc aagcgagtca taaaaatctt
      841 ggcaataacc accaaggtct tcaacataat gatacttaca aactgccgtg cacatgcaag
      901 aacaaagcac gcccgaaagt ttgtaattta cccagtcaag ctggtccata aatttgtagg
      961 tgtcattgta gaggtccggc aaatgacgct taatccacat gacttttggc acaaaccatt
     1021 ccggacttgc ctcgccaccg caataatcca aaaccgcgtg acctgttgca ttcagttcgg
     1081 cagtttcttc ggtagcacgc atatccatcc acatcaaagc attgcaaagt gggttgacat
     1141 ttttatcaat tgcgaaaaat gtcgaagcag ttgcatcaac agacacagtt gcgatgttgg
     1201 ccttgacttc gtcatccatt ttttccgaag catcttccag cgcaagctcc acgcattctc
     1261 accaaatatt tggatcctgt tccgcccaac caggcttagg atattcagtt tcccaagatt
     1321 ttgagacatc aatggcttgc ttgccttctt cgtcaaaaac accaatacgc actccttgag
     1381 tgcccatatc tatacccatg aaatatttca taagacccct tttctcgacc ctattattct
     1441 atcataaaaa cctgaaaata ttctcatagc aaaattcaag atacaaaata ttattaacat
     1501 aaaatttcat gtgttaaatt cgttgagtgt gtgttttcat tgttttctgc aaaaaaattg
     1561 cttcaaaaat gtgtcaattg cgttgagtgt gtgtttttat taaagaattt tctaaaaaat
     1621 tacaaagtaa aaattttgag ctgggctttt cttatctata aattaaattt gcgatgaaaa
     1681 agtacacata aaacgcaatc cacacatttt gagactaaat tttgtgtttt ttaatgaaaa
     1741 attggatatt ttgctaaaat taaagtatgg atgatttgtt aaaagatgta aaactttttg
     1801 tgcttgatat ggatggcact atttatttgg gtgaccagcc tattgagggt gcttttgatt
     1861 gagtgcgaaa aattgaggct gacccagacc gcgattacat ttttttcacg aataatgctt
     1921 cgaaggtgcc gagcatttat gttgagaagt tggcgaagct gggccttgat gttgatgaaa
     1981 gtaaaattgt tactgccggt gatgtttgtg ctcaattttt gaaggaaaat tatccgggtg
     2041 cgaaaatctt tttaaatggc actccggcac ttgaggaaaa ttgaaaagcg aagggactta
     2101 acctcgttaa agataagccg gatgtttgtg ttcaaagttt tgacacaacg ctaacctatg
     2161 aaaagcttga ccgcatctgt catttcattc gaaatggtgc ggatttcatc gcgactcaca
     2221 aagatataaa ttgcccgaca gaatacggct ttatgcctga ttgcggagcg atgtgcgagc
     2281 tcattaaatc ttcaactgga gtggcgccgc gcttcttcgg caagccttat aaagaaacgg
     2341 ttgatatgct gacctctatt actggcatcg aagcgaagca catggccttt gttggtgacc
     2401 gcctctacac ggatgttgcg accggcgtca acaatggtgc ggttggaatt ttggttctct
     2461 ctggtgaaag tacaattcaa accgtcgaag agagcgatgt gaagccaacc gcaatctata
     2521 attctatctg cgaaatctct taaaaggtgt aattattgcc aggcatgaaa tgcacctttt
     2581 tatgcgaatt tgtttcaggg gatgttgact gttgtgcggc cttcgagcag gt
//
LOCUS       DBNCDC010000115         2527 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_35294_length_2527_cov_7.707929, whole genome shotgun sequence.
ACCESSION   DBNCDC010000115 DBNCDC010000000
VERSION     DBNCDC010000115.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35294_length_2527_cov_7.707929"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="AC04VQ_07000"
     CDS             <1..843
                     /locus_tag="AC04VQ_07000"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIO9553842.1"
                     /translation="FMDKKEIQELKKFACEIRIGTVEAIKSRGFGHIGGTLSMADTLA
                     VLYKKIMNYDAKDPRKDDRDKLVCSKGHAGPAVYATLALCGFFPYDELKTLNQPHTKL
                     PSHCDKKLAGIDATTGSLGQGTSQAVGIALADKLQGRNCQTFLVVGDGELNEGQCWEA
                     AMFTAGKKLNNLVWIIDWNKKQLDGTLDEILPAFDFVEKFKAFGFDAQLVKGNDVEAL
                     FEAFSAPAGDKPKAIILDNVKGSGVKAVEETVANHSMQPEAEVFDQWLDALHADLEKI
                     EKEA"
     gene            845..1783
                     /locus_tag="AC04VQ_07005"
     CDS             845..1783
                     /locus_tag="AC04VQ_07005"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIO9553843.1"
                     /translation="MKVIYTGENDPRAMKDAITDASKKIMQDEKCVWLEADLAGCDGV
                     KALFGDSERYINCGIAEANMVGICAGLASNGMKPYCHSFAPFVSRRTFDQAFLSAGYA
                     HNPITVIGTDSGICAAFNGGTHMPFEDTGTYRMIPESIVCDASDYAMLYNFLIMAKDL
                     DGVKYVRTTRKVFQAFYEDDAKFEIGKGIVCREGSDCLIVACGIMMHEAMQAAEKLSS
                     EGIECAVIDPFTIKPLDIELIREYAKKCGKVVVAENHNKIGGLVSAVEDAIVDLGCKF
                     GYVAIEDEYGQVGPQDFLREEYKLTDTHITEVVKSL"
     gene            complement(1843..2226)
                     /gene="vapC"
                     /locus_tag="AC04VQ_07010"
     CDS             complement(1843..2226)
                     /gene="vapC"
                     /locus_tag="AC04VQ_07010"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF047863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HIO9553844.1"
                     /translation="MLDTNIVIYAIKKKPKKVLDHISAHKPGELCISSITYAELVHGV
                     EKSDHPDKNWIALSLMLSNFHILKFDEKAAEEYGQIYAELQKAGKPIGQMDALIAGHA
                     RSQNCVLVTNNLKEFKRVKNLNIEN"
     gene            complement(2325..2462)
                     /locus_tag="AC04VQ_07015"
     CDS             complement(2325..2462)
                     /locus_tag="AC04VQ_07015"
                     /inference="COORDINATES: protein motif:HMM:NF015947.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HIO9553845.1"
                     /translation="MQTATIFKSGNSQAVRIPKDYRFDEKELTINKVGDVVMLIPKKS
                     R"
BASE COUNT          708 a          552 c          540 g          727 t
ORIGIN      
        1 ttcatggaca aaaaagaaat ccaagaacta aagaaatttg catgcgaaat ccgcattggc
       61 actgttgaag ccattaaatc tcgaggcttc ggccacattg gtggcacgct gtcgatggcc
      121 gatacgcttg ccgttcttta caaaaaaatt atgaattacg atgcgaagga cccccgcaag
      181 gatgaccgcg acaagcttgt ctgctcaaaa ggacatgctg gtccagctgt ttatgcgacg
      241 ctcgcgcttt gcggattctt cccttacgat gagcttaaga ccttaaatca gcctcacacc
      301 aagttgccat cgcactgcga taaaaaactt gctggaattg atgctacaac agggtcgctc
      361 ggccagggaa cttcgcaagc cgttggaatt gctttggctg ataagttgca aggccgcaat
      421 tgccaaacat ttttggtagt tggcgatggc gaactcaacg aaggtcagtg ctgggaagct
      481 gcaatgttta ctgctggtaa aaagctaaat aatcttgttt ggattattga ctggaataaa
      541 aagcagctgg acggcaccct cgatgagatt ttgccggcct tcgattttgt tgagaagttt
      601 aaggctttcg gcttcgatgc tcaacttgtc aaaggcaacg atgtcgaggc tctcttcgaa
      661 gcgtttagtg ctcctgcagg cgacaaacca aaggcaatta ttttagacaa cgtaaaaggt
      721 agcggcgtta aggcagttga agaaactgtt gcaaaccact cgatgcagcc agaagccgag
      781 gtctttgacc agtggcttga tgctcttcat gctgaccttg agaagattga gaaggaggca
      841 taagatgaaa gttatttaca caggagaaaa tgatcctcgc gcaatgaaag atgccatcac
      901 cgatgcatct aaaaaaataa tgcaggatga aaaatgtgtt tggcttgaag ctgaccttgc
      961 aggttgcgat ggcgtaaagg ccctcttcgg agatagcgag cgctacatta attgtggaat
     1021 tgctgaagct aacatggttg gaatttgtgc tgggcttgcc tcgaatggca tgaagccata
     1081 ttgtcacagt ttcgcacctt ttgttagccg acgcactttt gaccaggcat tcctaagcgc
     1141 tggttatgct cacaacccaa tcactgttat tggaactgac tctggaattt gcgctgcttt
     1201 caacggtggc actcacatgc ctttcgaaga cactggcacc taccgcatga ttccggaatc
     1261 catcgtttgc gatgcctcgg attacgccat gctctacaac ttcctaatta tggcgaagga
     1321 tcttgacggc gttaaatatg ttcgcaccac tcgcaaggtt ttccaagctt tctacgaaga
     1381 cgatgccaag tttgaaattg gtaagggcat tgtttgtcgc gaaggcagcg attgcttgat
     1441 tgttgcttgc ggaattatga tgcacgaggc tatgcaagcc gctgaaaaac ttagcagcga
     1501 aggcatcgaa tgcgctgtta tcgatccatt taccattaag cctctcgaca ttgagcttat
     1561 tcgcgaatac gccaagaagt gtggcaaagt tgttgttgct gaaaaccaca acaaaattgg
     1621 tggtcttgtt agtgctgtcg aagatgcaat tgttgacctt ggttgcaaat ttggctatgt
     1681 tgccatcgaa gacgaatatg gccaagttgg tcctcaagac ttcctccgcg aagaatacaa
     1741 actcaccgac acccacatca ccgaagtcgt caaatcccta taaagatgta gactaagggg
     1801 tcggatgtag aaatacctcc gaccccaaac ttactacttt gttcagtttt ctatatttag
     1861 gtttttcact ctcttaaatt ctttaagatt attcgttaca agaacacaat tttgagatct
     1921 agcatgtcca gcaattaaag catccatttg accgattggc ttaccagctt tttgaagctc
     1981 tgcataaatt tggccatatt cttcagcagc cttttcatcg aattttaaaa tatgaaaatt
     2041 cgaaagcatc aaactaagcg caatccaatt cttatcagga tggtcactct tttccactcc
     2101 atgaacaagc tcagcatatg ttatagacga aatgcataat tctcctggct tatgagcaga
     2161 aatatgatcc aacaccttct taggcttctt cttaatagcg taaataacta tatttgtgtc
     2221 cagcatatac attataatgc ttccctcttt tgctccgaag acacagcacg tccttctttc
     2281 atgaagtcat cagtaaatag atctatccct aatatgaaag aggctcatct tgatttttta
     2341 ggtattaaca tcacaacatc tccaactttg ttgattgtta attccttttc atcaaatcta
     2401 taatccttag ggattcttac agcttggcta tttccacttt taaaaatagt tgcagtttgc
     2461 atgatgacca cctccttgtt aatagtatat acctatgtat atacattgtc aatatttatt
     2521 tacgagg
//
LOCUS       DBNCDC010000116         2498 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_35998_length_2498_cov_6.707736, whole genome shotgun sequence.
ACCESSION   DBNCDC010000116 DBNCDC010000000
VERSION     DBNCDC010000116.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35998_length_2498_cov_6.707736"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="AC04VQ_07020"
     CDS             <1..262
                     /locus_tag="AC04VQ_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553846.1"
                     /translation="NPACYVYGMGILSLVEAIEAKKVSSAIVQEFALDELIDFFIKSH
                     AQGMILGCTHFPYIKDPLEKLAKEMDPSFDIIDPADIMYENL"
     gene            566..1369
                     /locus_tag="AC04VQ_07025"
     CDS             566..1369
                     /locus_tag="AC04VQ_07025"
                     /inference="COORDINATES: protein motif:HMM:NF015159.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIO9553847.1"
                     /translation="MKKFIALVLAVVLVLSFTACGAKEEGKTDNVISIAASPTPHAEI
                     LAVAKEALAKEGFELNIIEYDDYVQPNMVVDSGEVFANYFQHVPYLDDFNAQNGTKVV
                     SLCGVHVEPMAIYAGKANDLGAIADGAQIAVPNDTTNEARALLLLEANGIIKLDPNAG
                     ITATKNDVIENPHNVDIVEVEAAQVPNVRQDVDYAVINVNYALAAGLNPVKDSLAIED
                     SASAYVNIVCVKEGNENDPKAQALVKACQSPEVKAFIEKTYDGAVVCVF"
     gene            1385..2383
                     /locus_tag="AC04VQ_07030"
     CDS             1385..2383
                     /locus_tag="AC04VQ_07030"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HIO9553848.1"
                     /translation="MIKIEHLSKQFTSGSLTVDALKDINLTINDGDIFGIIGMSGAGK
                     STLVRCINMLERPSEGTINVNGKDMGSLSDAELREARHDITMIFQAFNLLKQRNCLKN
                     ICFPMELTGVPKAQAEKRALELLEIVGLPDKAKAYPAQLSGGQQQRIAIARALATDPK
                     VLLCDEATSALDPNTTHSILSLLKDINEKMGITIVVITHQMSVVQEICNKVAILDDGV
                     IVEEGFVKDVFSAPKSDAAKRLVFPGMVDKEISDPSNERKIRLVYKDVQVISRPIIAS
                     MSADLGVFASVLAADTQKVSGDNYGTMLISVPNSQYEKAVSYLNTVPGIVVKGVEE"
BASE COUNT          833 a          535 c          492 g          638 t
ORIGIN      
        1 aaatcccgct tgctatgtct atggcatggg cattctttcc ttggtagaag ccattgaagc
       61 gaaaaaagtc agcagcgcca tcgtccaaga atttgcccta gacgagctaa ttgacttctt
      121 tattaaaagt cacgcccaag gcatgatctt aggttgcacc catttcccat acattaaaga
      181 tccgctcgaa aagcttgcca aagaaatgga tccgtcattt gacatcatcg acccagcaga
      241 tataatgtat gaaaatttgt aaaataccta cttgacacca tcactatggt gtgctaaagt
      301 atttggtaat tacaaacaaa aaggaagtaa aaatgaagtt taacgcaaac aaaaacagca
      361 tgatgcttat ggacatgtgc atggctatgc caatgcgcaa aattttgcgc gcagttaaaa
      421 gtcaaatact ttcctaagta aattatatta gtgttatcaa actcggaaag tgctttggct
      481 ttccgagttt tcttttttca taacagacag gcccgaaaac aaagcatttt tcgaatatca
      541 ttttttatta aaaggagata tagaaatgaa gaaatttatc gcattagtac ttgcagttgt
      601 attagtactc agcttcaccg cttgtggagc aaaagaagaa ggaaaaacag acaatgtaat
      661 ttccatcgca gcatcaccta cacctcatgc agaaatctta gcagtagcta aagaagctct
      721 tgcaaaagaa ggattcgaac ttaacatcat cgaatacgac gattacgttc agcctaacat
      781 ggttgtagac tccggcgaag ttttcgctaa ctacttccag cacgttccat accttgatga
      841 cttcaatgca cagaatggaa caaaagttgt aagtctttgc ggcgttcacg ttgaaccaat
      901 ggctatctat gcaggaaaag ctaacgacct cggcgcaatc gctgatggcg cacagattgc
      961 agttcctaac gatacaacaa acgaagcaag agcactcctc ttactcgaag caaacggcat
     1021 catcaagctc gatcctaacg caggcatcac agcaacaaag aatgacgtta tcgaaaatcc
     1081 acacaacgtt gatatcgttg aagtagaagc agctcaggtt ccaaacgttc gtcaggatgt
     1141 tgactatgca gtaatcaacg ttaactatgc acttgccgca ggcctcaatc ctgttaagga
     1201 ttcccttgca atcgaagaca gtgcatcagc ttacgttaac atcgtttgcg taaaagaagg
     1261 aaatgaaaat gatccaaagg cacaggcact cgttaaagct tgccagagcc cagaagtaaa
     1321 agcattcatc gaaaagacat atgacggcgc agtagtttgc gtattctaat ttaactaaaa
     1381 aacaatgatt aaaatcgaac acttaagcaa acaatttaca tcaggcagtt taacagtcga
     1441 tgcgctaaaa gatattaatc ttacgatcaa cgatggagac atcttcggca tcatcggtat
     1501 gtccggagca ggaaaatcca ctttggttcg ctgcattaat atgcttgaaa gaccaagtga
     1561 aggcacaatc aatgtaaacg gcaaagacat gggaagcctc agcgatgctg aacttagaga
     1621 agctcgccat gacatcacca tgatctttca ggccttcaac ttacttaagc aaagaaactg
     1681 tttaaaaaac atttgcttcc ctatggaact taccggagtc ccaaaggctc aagcagaaaa
     1741 gcgcgcctta gaacttcttg aaatcgttgg acttccagac aaagcaaaag catatccagc
     1801 acagctctcc ggaggccagc agcagcgaat agctatcgct cgcgcgctag ctacagaccc
     1861 aaaggttcta ctttgcgacg aagcaacatc tgcccttgat ccaaatacca ctcattcaat
     1921 tttatctttg cttaaagata ttaacgaaaa gatgggaatt accattgtgg taattactca
     1981 ccagatgagc gtagttcaag aaatctgcaa caaggttgcg attctcgatg acggtgtaat
     2041 agtagaagaa ggttttgtaa aagatgtctt ctcagcgcca aaatcagatg ctgcaaaacg
     2101 ccttgtattt cctggcatgg tcgataaaga aatttccgat ccgagcaatg agcgaaagat
     2161 acgtctcgtt tataaagatg tacaagtcat cagcagacct atcatcgcca gcatgtccgc
     2221 agacctcggt gtgtttgcta gtgttctagc agctgatacc caaaaggttt caggtgataa
     2281 ttatggaaca atgttaatat cagttccaaa cagccaatac gaaaaagctg tatcatattt
     2341 aaacacagtc ccaggaatag ttgtgaaagg agttgaagaa taatgtattc aggcattttt
     2401 tcagcagaat caattagcga agcactcctt tgcttaaaga cagaaattcc ttttgcaatt
     2461 tgggaaacct tctatgtaac aattttagca acagctct
//
LOCUS       DBNCDC010000117         2427 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_37810_length_2427_cov_6.344435, whole genome shotgun sequence.
ACCESSION   DBNCDC010000117 DBNCDC010000000
VERSION     DBNCDC010000117.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37810_length_2427_cov_6.344435"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            93..362
                     /locus_tag="AC04VQ_07035"
     CDS             93..362
                     /locus_tag="AC04VQ_07035"
                     /inference="COORDINATES: protein motif:HMM:NF028500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553849.1"
                     /translation="MPQVSDARSEALGALGVKTIRDLLFLFPERYIDMSEVSTILDAK
                     IGFNYTIAAVVHSVEEKEPKPGLKLIEITLTDKTGTLIATAFNQK"
     gene            363..542
                     /locus_tag="AC04VQ_07040"
     CDS             363..542
                     /locus_tag="AC04VQ_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553850.1"
                     /translation="MLDTLKVNDKVAVAGKVEFNYGFKRITNPFIHVLDEGDTSQDVA
                     KVVSIHPSNEKISSS"
     gene            630..977
                     /locus_tag="AC04VQ_07045"
     CDS             630..977
                     /locus_tag="AC04VQ_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553851.1"
                     /translation="MDYFQALRAVHFPNSMKELNLAKRTIKFSEVLLQQIYLSKDNAS
                     NSQLFVCEFCSDKIKKSVESISLFKDSGVQVLMLTSSSLMLAQYKQKFNEKFSDLHVA
                     FQIIDEGTSKENR"
     gene            1035..1400
                     /locus_tag="AC04VQ_07050"
     CDS             1035..1400
                     /locus_tag="AC04VQ_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553852.1"
                     /translation="MLNENIESKNLAVLVIDEKDQFSPQEISKAKSFLKTESDEICLT
                     TRPLTKSLATLLYPNSAHTRVAYRETHKAQTFVHENDLRMNAYEAAVNGIKSGKQCFV
                     ITPELDFKIDEIVNKIFNG"
     gene            1443..1766
                     /locus_tag="AC04VQ_07055"
     CDS             1443..1766
                     /locus_tag="AC04VQ_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIO9553853.1"
                     /translation="MALLNDFQDGKIEVLICSREIDFVIEPNSDVVAIVENSDRLGSS
                     KLHQIRNFILKKFDDSQLHLICFSKSKLAISRLKTFSEIMDGASVVAKDLEGRNDASK
                     LGFNK"
     gene            1925..>2427
                     /gene="rsmD"
                     /locus_tag="AC04VQ_07060"
     CDS             1925..>2427
                     /gene="rsmD"
                     /locus_tag="AC04VQ_07060"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIO9553854.1"
                     /translation="MRIIAGKYGGRKIDVPKGLEVRPTIDRVREAMFSSLISIYGSLD
                     GASVLDAFAGSGALGFEAISRGAKNLISFENNKRNYENLVENYQMLKDDDSNIKLLFA
                     DVKKAKFEHVCQNIKFDLLFFDPPYENLPSDVLDILKALIKVDAISKGAVIIYEHASK
                     DSFDAYV"
BASE COUNT          814 a          345 c          466 g          802 t
ORIGIN      
        1 gaaattgatc atcaagaagg tgggcaacct ctctatccaa ttctcttttc attggagtag
       61 ccattttaag gctagataca cccgtttcaa aattgcctca agttagtgat gctcgaagcg
      121 aagctcttgg cgcacttggt gtaaaaacaa ttcgcgattt gttatttctt tttccagagc
      181 gctatattga catgagcgaa gttagcacaa ttcttgatgc aaaaattgga tttaattaca
      241 ctattgcagc tgttgttcat tctgttgaag aaaaggaacc aaaaccaggt ttaaaattaa
      301 ttgaaattac gcttaccgat aagacaggaa cattgattgc tacggccttt aatcaaaaat
      361 gattgcttga cactcttaaa gtaaacgata aagttgctgt tgctggaaaa gttgaattta
      421 attatggttt taaaaggatc acgaacccat ttattcatgt attagatgaa ggtgacactt
      481 cacaagatgt tgcaaaagtt gtttcaattc atccttcgaa cgaaaaaatt tcctcctcgt
      541 gagttggaaa tattgttcaa aatgcatttg aatatttagg ttcactagat tctattattc
      601 caaaaacatt agttgagaaa tacaagctaa tggattattt tcaagctctt cgcgctgttc
      661 attttccaaa ctcaatgaaa gagctaaact tggctaaaag aactattaag tttagtgaag
      721 ttttattaca acaaatatat ttgtcaaaag acaacgcatc aaattctcaa ctttttgttt
      781 gtgagttttg cagcgataaa attaaaaagt ctgttgagtc aatttcttta tttaaagata
      841 gtggtgtgca agttttgatg ctaacttcgt cttcgcttat gctagctcaa tataagcaaa
      901 aatttaatga aaaattcagt gatcttcacg ttgcttttca aattattgat gaaggcacaa
      961 gcaaagagaa tcgatgaaaa gcaatttatg attttaagaa tggagattta gatttaattg
     1021 ttggtacgca tgaattgctg aatgaaaata ttgagtcaaa aaatttagct gttttagtta
     1081 ttgatgaaaa ggatcaattt agtccgcaag aaatttcaaa ggctaaatca tttttaaaaa
     1141 ctgagtcgga tgaaatttgt ttgacaactc gcccactaac aaaatcgttg gcaactttgt
     1201 tatatccaaa ttcggcacac acgagagttg cctatagaga aactcataag gctcagactt
     1261 ttgttcacga aaacgatctt cgcatgaatg cctatgaagc agcagttaat ggtattaaaa
     1321 gtgggaagca gtgtttcgtt attactccag aattggactt taaaattgat gaaattgtta
     1381 acaaaatttt caatggttga aatattggaa ttattaaaag tgatacttca acaaaagaag
     1441 cattggcttt gttaaatgat tttcaagatg gaaagattga agtcttaatt tgttctcgtg
     1501 aaattgattt tgttattgaa ccgaatagcg atgttgttgc tattgttgaa aattctgaca
     1561 ggcttggttc aagtaagttg catcaaattc gaaatttcat tttgaagaag tttgacgatt
     1621 cgcaacttca tttaatttgt ttttcaaaat caaaattagc aatcagtaga ttaaaaactt
     1681 tttctgaaat tatggatggc gcaagcgttg ttgctaaaga tttagaaggc agaaacgatg
     1741 cttcgaaact tggatttaac aagtgaggtt ttgatattct aaagttgatt aatgttgaaa
     1801 aagattctgc aattatagag actgcaaaca aagattcact agaaatatta agtgttgatc
     1861 cgcaactaaa aaatgcggag aataagttgc tatcatttga gatgtcaaga gtgtttgaaa
     1921 gctaatgaga attattgctg gaaaatatgg tggaagaaaa attgatgtcc caaaaggact
     1981 tgaggttcgt ccaactattg atagggttcg cgaagcaatg tttagctcgc tcattagtat
     2041 ttacggcagt ttagatggcg caagcgtttt ggatgctttt gcaggaagtg gtgcgcttgg
     2101 cttcgaagct atttcacgtg gtgcaaaaaa tctaatttct tttgaaaaca ataagcgcaa
     2161 ctatgaaaac ttggttgaga actatcaaat gcttaaagat gatgattcaa acattaagct
     2221 tttgtttgct gatgttaaaa aagcaaagtt cgaacacgtt tgccaaaaca taaaatttga
     2281 tcttttgttt tttgatccgc cttatgaaaa tttgccaagc gatgtcttgg acattttaaa
     2341 agctttaatt aaggttgacg caatttctaa aggcgcggtt attatttatg aacatgcaag
     2401 caaggatagt tttgatgctt atgttga
//
LOCUS       DBNCDC010000118         2390 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_38797_length_2390_cov_6.901499, whole genome shotgun sequence.
ACCESSION   DBNCDC010000118 DBNCDC010000000
VERSION     DBNCDC010000118.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38797_length_2390_cov_6.901499"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="AC04VQ_07065"
     CDS             <1..242
                     /locus_tag="AC04VQ_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553855.1"
                     /translation="QERYFTLIGRASRFYINSNFNKVYCGHVQREICNLDIVHDCAVV
                     PYPDKNLLYVGKAFIVLNKNEAINSIKENDILDLL"
     gene            complement(434..1054)
                     /locus_tag="AC04VQ_07070"
     CDS             complement(434..1054)
                     /locus_tag="AC04VQ_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553856.1"
                     /translation="MSLLSLNEKKRIRIKRMASGDFEIRLKNPAWAKDAYAAKNINII
                     DKRTINFLFGEIACGNPLLRLSDIQNYAHARSYDFMSSYLAWHSLLTDEVNEFANFKS
                     IYDKTRHILFGISITLGLISIALGIVSLEIITPIIGIVAAIVIGYIGNSLRNKVYFKD
                     SKGKKIDAQDLNIGLAEYTETIDKFRIAAIKTIRQSVLDAQDIISK"
     gene            complement(1082..1429)
                     /locus_tag="AC04VQ_07075"
     CDS             complement(1082..1429)
                     /locus_tag="AC04VQ_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553857.1"
                     /translation="MVSLKSEVVPGNSAAEIRIVFPTAWLTNLDMGSDISQQNQAKLT
                     TILKEESVWRDYRTDAVNKLILPIAFTGFCILVLVACLIILVIYRRRFLTGALSQQKL
                     KEMHPCLLVRLKN"
     gene            complement(1475..1609)
                     /locus_tag="AC04VQ_07080"
     CDS             complement(1475..1609)
                     /locus_tag="AC04VQ_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553858.1"
                     /translation="MMDSYDVSLNVTVPVGKGSIVNPLGKVSSIETNSESTTERNIYA
                     "
     gene            complement(1631..1753)
                     /locus_tag="AC04VQ_07085"
     CDS             complement(1631..1753)
                     /locus_tag="AC04VQ_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553859.1"
                     /translation="MQAMTGTPDINLWSSTKAIINLNYTIERAATIYKDVADFQ"
     gene            complement(2003..2293)
                     /locus_tag="AC04VQ_07090"
     CDS             complement(2003..2293)
                     /locus_tag="AC04VQ_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553860.1"
                     /translation="MKSTKTNISKKIVSIFICIALAMSFCFASPRKAFATSTAETLSW
                     KSDINASIGTDGAISVSEQKIIDITPFINKAKSYKVGTNEAPNTAPLLHPLV"
BASE COUNT          728 a          414 c          461 g          787 t
ORIGIN      
        1 atcaagaacg ttattttaca ttaataggaa gagcttcaag attttatatc aattcaaatt
       61 tcaataaagt ttattgtgga catgtacaac gtgaaatttg caatttagat atcgtacatg
      121 attgcgctgt tgtgccatat cctgataaga atttgttata tgttggcaaa gcttttattg
      181 tattaaacaa aaatgaagca attaactcta taaaagaaaa tgatattctt gatttattgt
      241 gaaagtttta taaaaatggc aatctgtcat tgtattctta cgaaatacca caagttgtag
      301 aaatagtaaa tgagcttcca aaaaatgaag ccgataaaat tgattacgaa ttactaaaaa
      361 atacagcatt aaaatagact tgcgaatcaa aataattcaa attaaataat tactcagaaa
      421 tgttaatgag gtttcatttg ctgatgatgt cttgcgcgtc aaggacagat tggcgaattg
      481 ttttgatggc tgcgatgcgg aatttgtcga ttgtttcagt gtattcggca aggccgatgt
      541 taagatcctg cgcatcaatt ttctttcctt tggaatcttt gaagtagact ttatttcgca
      601 ggctgtttcc tatataccca ataacaattg ccgcgacaat tccaatgatg ggtgtaataa
      661 tttctaagga tacaattccc aaagcaatag aaattagccc aagcgttatt gaaatcccaa
      721 acaatatgtg acgcgtttta tcataaattg atttaaagtt agcaaattca tttacttcgt
      781 cagttaaaag cgaatgccac gcaaggtaac ttgacatgaa atcataagat cttgcgtgcg
      841 cataattttg aatatcagaa agtcgaagca gcggattgcc acatgcaatt tcgccaaaga
      901 ggaaattaat tgtgcgcttg tcaattatgt tgatattctt tgctgcataa gcatccttag
      961 cccacgcagg gtttttaaga cgaatttcaa agtcgcccga tgccatgcgt ttaatgcgaa
     1021 tgcgtttctt ttcgttaaga gacaaaaggc tcacaactaa gtcatgagca aattcatggt
     1081 ttcaattttt aagacgaaca agtaggcaag ggtgcatctc ttttaatttt tgttgagaga
     1141 gggcaccggt taaaaatctt cgtctgtaaa taaccaaaat tattaagcaa gcaacaagaa
     1201 ccaaaatgca aaatccggta aaggcaattg gaaggattaa tttgtttacg gcatcggttc
     1261 tgtaatcgcg ccaaacggat tcttctttta gaattgttgt tagcttggct tgattttgtt
     1321 gtgaaatatc acttcccata tcaaggttgg taagccatgc tgttgggaat acaattcgaa
     1381 tttcagctgc cgaattgcct ggaacaactt cgctttttag agagaccaat ccattggggt
     1441 ttaagtcaac aacgccagag cttgatccgt ggcctcatgc gtaaatgttt ctctctgtag
     1501 tgctttctga attagtttct atacttgaaa cttttccgag cgggttaacg atgcttcctt
     1561 ttcctacagg aactgtcacg tttaagctaa catcataaga atccatcatt catgtttctg
     1621 ggcaatattc tcattgaaaa tctgcaacgt ctttataaat ggttgcggct cgctcaattg
     1681 tgtagttaag atttattatt gcttttgttg acgaccataa attaatgtcc ggagttccag
     1741 tcatagcttg cattatttca aaacattgtt gagagctcaa ttggctcgag tttacaagtt
     1801 ctgtgtataa atacattttt ttgtcagctt cgctgtaagt gaaagttgga gttgttgggc
     1861 tgtcttcgcc ttttcattta gaaagaaaag ttgcgttttc aaaacctctt cacgctccaa
     1921 tgacggtatc tgtgtcggac aaaattgcaa ttcttgcatc gcttaaagtg atgtcgcttt
     1981 cttccggcaa aaatctgtag cctcaaacta atgggtgcaa tagaggagca gtgtttggag
     2041 cttcgttagt gccaacctta tatgattttg ctttatttat gaaaggagtg atgtcgataa
     2101 ttttttgttc acttaccgaa attgctccat ccgttccaat cgaagcgtta atatcagatt
     2161 tccaagaaag tgtttctgcg gtcgatgttg caaatgcttt acgcggcgaa gcgaagcaaa
     2221 agctcatcgc gagtgcaata caaataaata ttgaaacaat ctttttgctt atgttagttt
     2281 ttgtactctt cataaaaaac tggaactgcc atgtcaaaat ccgaggcgtc tttatctagc
     2341 cttctttggc gtaaatacgt tccactaaaa tgtgaattat agcagtatag
//
LOCUS       DBNCDC010000119         2361 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_39672_length_2361_cov_7.591067, whole genome shotgun sequence.
ACCESSION   DBNCDC010000119 DBNCDC010000000
VERSION     DBNCDC010000119.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39672_length_2361_cov_7.591067"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(148..579)
                     /locus_tag="AC04VQ_07095"
     CDS             complement(148..579)
                     /locus_tag="AC04VQ_07095"
                     /inference="COORDINATES: protein motif:HMM:NF015450.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553861.1"
                     /translation="MAEVDNLKKLFQDAISSSNLEVKGDVDVALERPKDDANGDFAST
                     VALRLSKELGKNPREIAQAIVDNFPQNSIVNKIEIAGPGFINVYLNNASVSSIVDRIR
                     SEKMDFAKNCAQGKVRDIDLKVNLEYISANPTGPMHVGHGR"
     gene            complement(583..1158)
                     /gene="hpf"
                     /locus_tag="AC04VQ_07100"
     CDS             complement(583..1158)
                     /gene="hpf"
                     /locus_tag="AC04VQ_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIO9553862.1"
                     /translation="MELTVTGRKFAITDWMKEYCDEKIGNAARVFDIDPLTIEVVLTQ
                     EKNPANPTPAKCEVTLRAGGHVIHVEENEEEIHAAIDVASAKVTRQLRKFKTRVVDKR
                     NRGAEKEAARAAEGAVGDELDLDALMADLSEDDIVRTKEVELRPMKVEEAMFDFDLLG
                     HDFYMFIDEEDDKFAVLYTRKDGGYGLLKQA"
     gene            complement(1180..2040)
                     /locus_tag="AC04VQ_07105"
     CDS             complement(1180..2040)
                     /locus_tag="AC04VQ_07105"
                     /inference="COORDINATES: protein motif:HMM:NF014679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIO9553863.1"
                     /translation="MADKKVVISADSTCDLNEELKKQYNVQYYPFYINLRDKDYKDGV
                     DITLEEMFNAYYEDKSLPKTAASSIGEFTEHFKQFTDQGAEVVHISLGHALSSAFANA
                     SMAAEQIDGVYVVDSCNLSNGSGQLVIRAARMAEEGKSAKEIVDELEKIKPHVHTSFV
                     LDTLDFMAAGGRCPAIAAKAAGMLKCKPYLSVDNSDGSLHIGKMYRGKQEMVLRKYIE
                     DELGKYDNLVLDDIFLCNSTGFEDSYIDELKDLILKIAPFKRVHKTLSNGTISSHCGP
                     RCLGILFVTD"
     gene            complement(2119..>2361)
                     /locus_tag="AC04VQ_07110"
     CDS             complement(2119..>2361)
                     /locus_tag="AC04VQ_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553864.1"
                     /translation="HPTVLVSLTPAISKDPRYTAITNSFLGIGQNLGAMSVGAISGRI
                     LEAYGFEVLSVVYVCFAVVIVIGTFLIYLGIRKNFA"
BASE COUNT          728 a          552 c          379 g          702 t
ORIGIN      
        1 atcttgaccg agctcttgta aatatctaac cgaaatagaa ttgccaaaca aattcatttg
       61 aacgccgtgg tcatttaaat agaatttctc gtaaacatca taaccagcgt gacgcataat
      121 ttttgcgata ctatcaccaa gagccgctca ccttccgtga cctacatgca tagggccagt
      181 tgggtttgca gaaatatatt caaggttaac ctttaaatcg atatcacgaa ccttgccttg
      241 agcacaattt ttagcgaagt ccattttttc acttcggatc ctatcaacaa tgctacttac
      301 acttgcattg ttaagataga cgtttataaa acctggacct gcaatttcaa tcttgttgac
      361 aattgaattt tgaggaaaat tatcaacaat tgcctgcgca atttctcgag gattttttcc
      421 aagctcttta gaaagacgaa gcgcaaccgt gcttgcaaaa tctccattcg catcatcttt
      481 aggacgctca agagcaacat ccacgtcacc cttaacttcc aaattcgaag aagatattgc
      541 atcttgaaat aattttttta aattatcaac ttcagccatt aattaagcct gctttaaaag
      601 tccataaccg ccatctttgc gagtatatag aactgcaaat ttgtcatcct cttcgtcaat
      661 aaacatataa aaatcatgac caagcaaatc gaagtcaaac atcgcctctt caactttcat
      721 aggacgaagc tcaacctcct tcgtgcgaac aatgtcatct tcagaaaggt cagccattaa
      781 agcatccaag tcaagctcat cgccaactgc accttcggca gctcgagcag cttctttttc
      841 agcaccgcga tttctcttgt caacaacacg cgtcttaaac ttgcgaagct gacgcgtgac
      901 cttcgccgat gcaacatcaa ttgcagcgtg aatttcttct tcgttttctt caacgtgaat
      961 aacgtgaccg ccagcacgaa gagtaacctc gcacttagca ggagttggat tggcaggatt
     1021 cttctcttga gttagcacaa cctcaatagt tagaggatca atatcaaaaa ccctggcagc
     1081 attgccgatt ttttcatcgc aatattcttt catccaatct gtgatagcaa attttctgcc
     1141 tgttactgta agttccataa taaccccaat ctcttaattt caatctgtaa cgaacaaaat
     1201 acccaagcac cttggtccac aatggcttga aattgtgcca ttagaaagcg ttttgtgaac
     1261 acgcttaaaa ggtgcaattt ttaaaattaa atctttgagc tcatcaatat aactgtcttc
     1321 aaaaccagtt gaattacaca aaaatatgtc atccaaaacc aagttgtcat attttccaag
     1381 ctcatcttca atatatttac gaagaaccat ctcctgcttt ccgcgataca tcttaccaat
     1441 atgaagcgaa ccgtctgaat tgtccacaga aagatatggc ttgcacttaa gcatgcctgc
     1501 agcctttgca gcaattgcag gacatctgcc gcccgccgcc ataaaatcaa gagtgtcaag
     1561 cacaaaagat gtatgaacgt gaggcttaat tttttcaagt tcatcaacaa tctctttggc
     1621 ggacttccct tcttcggcca ttcttgcagc gcgaataaca agctgtccag aaccgtttga
     1681 caaattacaa ctatccacta cataaacgcc gtcgatctgt tcggcagcca tgctcgcatt
     1741 tgcaaacgcc gaagacaatg catgtcccaa actgatgtgg acaacttcgg caccttgatc
     1801 tgtaaattgc ttgaagtgct cagtaaattc gccaatacta cttgcggcgg tcttcggcaa
     1861 ggatttatct tcgtaataag cattgaacat ctcttctaaa gtgatgtcaa caccatcttt
     1921 ataatcttta tcacgtaaat tgatatagaa cggataatac tgaacgttat attgcttctt
     1981 tagttcttcg tttaaatcac acgtgctatc agcacttatt actacttttt tatctgccat
     2041 aatacttcct ttaactaaat ttcattttaa cataaaattg cgaatttatt gccacgcaat
     2101 aattcacttt agtaaagatt atgcaaaatt ttttctaatt ccaagataaa ttagaaacgt
     2161 gccaattact atcacaacag caaagcaaac ataaaccacg cttaaaactt cgaagccata
     2221 agcttcaaga attcttccgc tgattgctcc aacagacata gctcctaagt tttgacctat
     2281 gcctaaaaag ctgtttgtta ttgcagtgta gcgaggatcc ttcgaaatgg ctggagtcaa
     2341 acttacaaga actgttggat g
//
LOCUS       DBNCDC010000120         2349 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_40023_length_2349_cov_6.959024, whole genome shotgun sequence.
ACCESSION   DBNCDC010000120 DBNCDC010000000
VERSION     DBNCDC010000120.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40023_length_2349_cov_6.959024"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="AC04VQ_07115"
     CDS             <1..495
                     /locus_tag="AC04VQ_07115"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIO9553865.1"
                     /translation="LDMMIHDFDMARFLADCEPVEVYAEGAVLVDKAIGEAGDVDTAL
                     VTMRMEDGSLVSISNSRRAAYGYDQQAEIFGSKGAVKCGNDASSQAVLSDENGVTAEK
                     PLFFFLERYMDAYADEIKQFIDAVENDTDTPVNVMDGLEAVRIGIAATKSCKEHRPVK
                     MSEI"
     gene            648..2024
                     /locus_tag="AC04VQ_07120"
                     /pseudo
     CDS             648..2024
                     /locus_tag="AC04VQ_07120"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2024..>2349
                     /locus_tag="AC04VQ_07125"
     CDS             2024..>2349
                     /locus_tag="AC04VQ_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014795.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIO9553866.1"
                     /translation="MASTTRMTTAQAIVKFLDNQYVSFDGQENKFVHGFFTIFGHGIA
                     VGLGEALDSDAGRLHVMQGRNEQGMCHAAIAFAKQSNRRQIIACSSSIGPGAANMVTA
                     AATATV"
BASE COUNT          631 a          414 c          530 g          774 t
ORIGIN      
        1 cttgatatga tgattcatga ttttgacatg gctcgtttct tggcagattg tgaaccagtt
       61 gaggtttacg ccgaaggtgc tgttcttgtt gataaagcaa tcggcgaagc tggagacgtt
      121 gacacagcac ttgtaacaat gagaatggaa gatggttctt tggtttcaat tagcaattca
      181 cgtcgtgctg cttatggcta tgatcagcaa gctgaaatct tcggaagcaa aggtgctgtt
      241 aaatgcggta acgatgcttc gtcacaagca gttcttagcg acgaaaatgg tgttacagct
      301 gaaaaaccat tgtttttctt cttggagaga tatatggatg cttatgctga tgaaattaag
      361 caattcattg atgctgttga aaacgacaca gatactcctg taaatgttat ggatggtctt
      421 gaggctgttc gtatcggtat tgctgctacg aagtcttgca aagagcatcg tcctgttaag
      481 atgtcggaaa tttaggagga gccatgcccc aaaatgaaaa attagaatca aagcaacacg
      541 agagggtcgc cgaaggtaaa tcacaagctc aaactggtgg tcacattaaa aaccttgttg
      601 gcgatatccg tcttaattga ggtactaggt ttgcttatgc tagtggtgat attgcttgta
      661 atatcgtatt cggcacaatt ggtgctgttt taaccttatt ttatactgac tatgttggta
      721 ttaatggtgc tgttgtggca accatcatgc ttatttgtag gtgctttgac ggcttttctg
      781 acttaatcat gggcttcatt gttgaacgaa cgaagtccaa atgaggtcag tcaagacctt
      841 ggattttgtg gatgtcgttc ccattttcta taggttgcgt cttgctgttt acggttccgc
      901 acttcgataa cgatttctta gtttatctat atgttttctt gacatatttc ttcgtttctg
      961 ttgtgtgcta cactgccgta aaccttccat acggttcttt gtcttcgatg atgacaagaa
     1021 atgcgaagga aaggcaatcg ctttctgtag ttcgtatggg tatgagccca attggaaaga
     1081 tcattgctgt tacacttaca acacctattg ttaaaaattt cttaggcgac gaccaagcag
     1141 catggattac tatcatgatt gtttgggcta ttgttgctga cattttattg ttgatttgtt
     1201 tctttaattg taaagaacaa gttgtaattc ctggccgcga agatccttcg aaggcaaaag
     1261 caactccaat tcttaaagga cttaaggcat tgtttagcaa ccagtatttc tgggctgttt
     1321 tagttctttg gatgattcaa agtgttgcat ttagcgtttc tggaactgcg ggtcagtatt
     1381 acacaaaata cgttttgtct ggcaacacag caggatggac agccagctta tttggattca
     1441 atcttgacgc tgagacatta cagtctattt ttgttgatgt cgaaattatt tcgatggtaa
     1501 tatttatttt cttatgcgct ccagttgttc gtaagtttgg taaaagaaac acagcattct
     1561 taggcgcaat tgttgctttg ggtgctcaat ttgtattctt gattaaccca atggatgtta
     1621 acatcatcct tcttacttgc gcattaagag gaattggctt agcgccacta aatgctgttg
     1681 tttttgcgat ggttggagat gctattgagt ttggccaatg aaaatcacat atcagacaag
     1741 aaggtcttgt atttgctgga ggttctgtag gtactaaagt tggtgccggt attgcttcgg
     1801 ctgcaattcc tgccttgctt gattggtctg gtcaaatcac atcaacttcg ggctttgcag
     1861 ctcagccaga aagcgtaatt caaatgattt caaacatcta ctttgctggt ccattgatca
     1921 ttgcaattat ggcgcttgta actttattct tatatagact cgataagaaa tacgatgata
     1981 ttgttgctga attagcagaa cgtgaagcga aaggacaatt ataatggcat caacaacaag
     2041 aatgacaact gcacaagcta ttgttaaatt tttagacaac caatatgtca gcttcgacgg
     2101 tcaagaaaat aagtttgtcc atggattctt tacaattttt ggtcatggta ttgctgttgg
     2161 actcggcgaa gctctagatt ccgacgctgg acgtcttcat gtaatgcaag gaagaaacga
     2221 gcaaggaatg tgtcatgctg ctatcgcatt tgcgaagcaa tctaacagac gacaaattat
     2281 tgcttgttca tcaagtattg gacctggtgc tgcaaatatg gttacagctg ctgcaactgc
     2341 aacagtaaa
//
LOCUS       DBNCDC010000121         2307 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_41325_length_2307_cov_5.603464, whole genome shotgun sequence.
ACCESSION   DBNCDC010000121 DBNCDC010000000
VERSION     DBNCDC010000121.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41325_length_2307_cov_5.603464"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(32..205)
                     /locus_tag="AC04VQ_07130"
     CDS             complement(32..205)
                     /locus_tag="AC04VQ_07130"
                     /inference="COORDINATES: protein motif:HMM:NF025423.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HIO9553867.1"
                     /translation="MAITFEGYERRIDKINGVLKEHGIKSLDECKEICDKAGIDPMAI
                     VKEVQPIAFDNAG"
     gene            complement(215..907)
                     /locus_tag="AC04VQ_07135"
     CDS             complement(215..907)
                     /locus_tag="AC04VQ_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HIO9553868.1"
                     /translation="MIYTAEVEKMCPVRKGADHGPAPIPQEGEWTKAKEIKDIKGLTH
                     GVGWCAPQQGACKLSLNIKDGIIEEALVETLGCSGMTHSAAMASEILIGKTILEALNT
                     DLVCDAINTAMREIFLQIVYGRSQTAFSEGGLPVGSNLDDLGKGLRSMVGTTYGTKAK
                     GPRYLELTEGYITKNALNKDNEIIGYEYINVGKMMDYIKEGMDANDALEKAKGTYGQF
                     EGAAKYIDPRSE"
     gene            complement(950..1729)
                     /locus_tag="AC04VQ_07140"
                     /pseudo
     CDS             complement(950..1729)
                     /locus_tag="AC04VQ_07140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(1748..2125)
                     /locus_tag="AC04VQ_07145"
     CDS             complement(1748..2125)
                     /locus_tag="AC04VQ_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553869.1"
                     /translation="MSKHYSHIITACAFLLMFVNVGFPSTSFGVYQPYIVAVPGMDHS
                     AGSVVVTVRNFASLVTIFFIAWYYRKLDCRMGIFLGTLATAIGFGIFAISNNLTTFVI
                     ASIVSGIGYGFAGTSAMTILIAR"
     gene            complement(2138..2281)
                     /locus_tag="AC04VQ_07150"
     CDS             complement(2138..2281)
                     /locus_tag="AC04VQ_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553870.1"
                     /translation="MDMFKAPMFGSRKPEKIAKFSEKSKADLDHIDEFIENLDIDSKM
                     FKS"
BASE COUNT          753 a          533 c          386 g          635 t
ORIGIN      
        1 tcaaggcaat agctgcaccc atagtatatg ctcaaccagc attatcgaaa gcaatcggct
       61 gaacctcttt aacaatagcc ataggatcaa taccagcttt gtcgcaaatt tctttgcatt
      121 cgtctaaaga cttaatacca tgctctttta gcacgccatt aatcttgtca attcttcttt
      181 catatccttc aaaagtaata gccatggctg cctcctattc gcttcgcgga tcgatgtact
      241 tcgccgcgcc ttcaaattgt ccataggtgc cttttgcctt ttcaagagca tcattggcat
      301 ccatgccttc tttaatataa tccatcattt tgcccacatt gatatattca tatccaataa
      361 tctcgttatc tttattaagg gcgttctttg taatgtaacc ttcggtaagc tccaagtaac
      421 gaggcccttt cgccttcgtc ccgtaagttg tgccaaccat agagcgaagc cctttgccca
      481 aatcatcaag gttagaacca acaggaagtc ctccttcgct aaatgcagtt tggctacgtc
      541 catatacaat ttggaggaaa atctcacgca ttgcagtgtt aatagcatca caaacaaggt
      601 ctgtattcag cgcctcaaga atagtctttc caatcaaaat ctccgaagcc atggcagccg
      661 aatgtgtcat tccgctacaa cccagtgtct ccaccagggc ttcttcgata attccatctt
      721 tgatgtttaa gctaagctta caagcacctt gttgtggcgc acaccagcca acaccatgag
      781 tcaagccctt gatgtcctta atctctttag cctttgtcca ctcgccttct tgagggattg
      841 gagcggggcc gtgatctgca cccttgcgaa cagggcacat cttttctact tcagctgtat
      901 aaatcatttt tcctccaata atttatccac actaattata aaccaattat tattttctag
      961 ggaataattt aataattatt attgctgaaa ttagaaccgc aattgccgaa gcaaagtaaa
     1021 atggaacgta agaacctgta aattcggcaa cgatgcctgg aatcattccg gttaaaaatc
     1081 ctcctccagt gtaggcaacc tgaaggtctc taacgcaact gtagcgagat tctggtgttg
     1141 ataaatcaac tgaccattga gcaacaccta cggtagtaat ggctgttcca aaaccaaaaa
     1201 ttgcagttga gatgtatggg aaaattccag tataaccagc agcaatacaa attagcaact
     1261 taccggttaa caaaattaca aaagcaacaa tggtcccttt taatgttcca agtttatcta
     1321 atagataacc caaaacaatt tttccaaaag ccaaagcaaa attagcaact gagactacag
     1381 cagctgccaa aacaggatct gcgccagaag aagtaaacaa aacacttaaa taaacaatgt
     1441 ctgacaggca tgctccgccc aaaagaaaaa caccgaatac atatagataa tacttccctt
     1501 taggaagctt gcgcttcgca ttccttttta actttaattg gtcgttggtc tttttgccat
     1561 ccttgctatt acgaataaaa attagcaaaa caaaagcagt tataagtgca aatacagcct
     1621 gaacagcaaa agcttgacct aattccccag ttgaaagaat agccaaaaca accattggaa
     1681 cacatacacc agacacacta ctaccaattg ttgcgatgcc gaaaggggtc cctgtatctt
     1741 tttcgtgtca ccttgcaatt aaaatagtca tcgccgaagt tccagcaaat ccataaccaa
     1801 taccagaaac aatggaagca attacaaaag tcgttaaatt attagaaatt gcaaaaattc
     1861 caaaaccaat ggctgtcgcc aaagtcccaa gaaaaatacc catgcggcaa tcaagttttc
     1921 tataatacca agcaatgaaa aagattgtaa ccaagcttgc gaagtttcgg acagtaacaa
     1981 ctacagaacc tgcagagtgg tccatgcctg gaacagctac aatataaggc tggtagacgc
     2041 caaaagaagt gctgggaaac ccaacattta caaacatcaa caaaaatgcg caagcagtaa
     2101 taatatgtga gtagtgctta ctcatttata aaatatatta gcttttgaac attttagaat
     2161 caatatctaa gttctcgata aattcatcaa tgtggtcaag atctgccttt gacttctctg
     2221 aaaacttcgc aattttttct ggtttacgcg aaccaaacat tggagcttta aacatatcca
     2281 aagttggaat tgcaatatga actcaat
//
LOCUS       DBNCDC010000122         2282 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_42123_length_2282_cov_8.715761, whole genome shotgun sequence.
ACCESSION   DBNCDC010000122 DBNCDC010000000
VERSION     DBNCDC010000122.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42123_length_2282_cov_8.715761"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(64..243)
                     /locus_tag="AC04VQ_07155"
     CDS             complement(64..243)
                     /locus_tag="AC04VQ_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553871.1"
                     /translation="MLKALIKKQASELFAILFLGAGRPASKKKKKKSSDRSSFVLKAT
                     LIGILFMVLFLGICL"
     gene            complement(224..955)
                     /locus_tag="AC04VQ_07160"
     CDS             complement(224..955)
                     /locus_tag="AC04VQ_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIO9553872.1"
                     /translation="MLKIDNLTKKYGDKVAVDNLTLHIKPGEVYGFIGSNGAGKTTTL
                     KACAGIHTFDEGTITVAGHSIKDKPIEAKKIFAYIPDNPDLYEYMTGIKFLNFVADIF
                     EVSADVRTKKFHELATTFGIEADLATPISDYSHGMKQKLALISAFCHDPKLIMMDEPF
                     VGLDPSAAFNLKEMMRKHCDNGGAIFFSTHVLEVAEKLCDKIAIIKGGKLIKSGTMEE
                     VKGDESLEEVFLELADGETNAESSN"
     gene            complement(971..1432)
                     /locus_tag="AC04VQ_07165"
     CDS             complement(971..1432)
                     /locus_tag="AC04VQ_07165"
                     /inference="COORDINATES: protein motif:HMM:NF018271.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIO9553873.1"
                     /translation="MYGFGGVACALIFYPLKNWLGKKIKVPFVNVILVFIISAIVCSL
                     MELIMGLATNADYSLWDYRTMWCNFMGQVCLQNSLGFGLAATIMTYVVYPLVKGAIAK
                     LPKDGANILFVGIVIFFTLLMVFYYINDFTGLLNDDVVKEAMQPNAYIFFR"
     gene            complement(1847..2092)
                     /locus_tag="AC04VQ_07170"
     CDS             complement(1847..2092)
                     /locus_tag="AC04VQ_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553874.1"
                     /translation="MAKKKSKKEPKLITKDADGNPIPKKFQNPKKLGFLRVLMISYGM
                     VPFVLLFCCFFITKEQFVLNAMTFNAIVDCIAYAIVF"
BASE COUNT          757 a          484 c          386 g          655 t
ORIGIN      
        1 aaataggaag aaatacattc aatcgaagcc cgcgctaacc aagccttcaa ccatcgccca
       61 cgctcaaagg caaatgccca aaaacaaaac catgaacaaa attccaataa gtgtggcttt
      121 tagaacgaag ctgctcctgt cgcttgactt tttcttcttc ttttttgatg caggcctgcc
      181 agcacccaag aagagaattg cgaagagctc gcttgcttgt tttttaatta gagctttcag
      241 catttgtctc tccatcagca agttcgagga aaacttcttc gaggctctcg tcccctttga
      301 cttcttccat ggttccgctc ttaattagct tgccaccttt aataattgca attttgtcgc
      361 aaagcttttc agcaacttcc aaaacgtgag tactgaagaa aattgcgcca ccgttatcgc
      421 aatgtttacg catcatttct ttaagattaa aagccgcact tggatccaaa ccaacaaaag
      481 gctcatccat cataataagt ttagggtcat ggcaaaatgc cgaaattaaa gcaagctttt
      541 gcttcatacc atgagaataa tcgctgatag gggtggctaa atctgcttcg atgccaaaag
      601 ttgtggcaag ttcatgaaac ttcttcgtgc gaacgtcagc actgacctca aaaatatcag
      661 ccacaaaatt tagaaactta atgccggtca tatactcata aaggtccggg ttatctggaa
      721 tgtaggcaaa aattttcttc gcttcgattg gcttatcttt tatcgaatgc ccagcaaccg
      781 tgattgtgcc ttcgtcaaat gtatgaatgc cagcacaagc tttaagcgtt gtagtttttc
      841 cagcaccatt actgccgatg aaaccataaa cctcaccagg cttaatgtgt aaggtcaaat
      901 tatcaacagc aactttatcg ccgtatttct tggttaaatt atcaatcttt aacatttctc
      961 tcctatcatg ctatctgaaa aatatataag cgtttggctg catggcttct ttaactacat
     1021 cgtcattcaa aagtccagta aaatcattga tgtagtaaaa aaccatcaaa agtgtgaaaa
     1081 atataacaat tcctacaaac aaaatgttgg cgccgtcttt cgggagcttc gcaattgcgc
     1141 ctttgaccag aggataaaca acataagtca taatggtcgc agcaagtccg aagccgaggc
     1201 tgttctgcaa gcaaacctga cccataaaat tacaccacat tgtgcggtaa tcccacaaag
     1261 agtaatcggc atttgttgca agccccatga taagctccat taagctacaa acaatagctg
     1321 aaattataaa aactaaaatt acattaacaa aaggaacttt aatttttttc ccaagccaat
     1381 ttttaagagg ataaaatatc aaagcacaag caactccgcc aaaaccataa atcggaaacg
     1441 ggaaaagtca atcgcttcaa atttgactgt taggatcata aattccagga agccaaccca
     1501 tgcgaactgg gaaacaatat gctgcttcca tccaatgccc tacaatgctg aacacacaaa
     1561 atatcataat taaatcacgt caaaaagccc aggtctttgg ctgataatag tcgcgctgaa
     1621 gcttaaattc tttactttcc ggctcaacta taaatggctg tgtcaaaagg tacttgcatc
     1681 ttttcgactt aaagaaatac aaaagaacta agccatcgcc aattacatct gtgataatag
     1741 ttaggtagtc tccagattgc aaagcaagcg ttaaagtaac aactcagtca aacagactta
     1801 atgcaagaac aacaaacttc gtgtccttct tccgcttcca aatcattcag aaaacaatgg
     1861 catatgcaat gcagtctaca atcgcattaa aagtcattgc gtttaaaacg aattgctctt
     1921 ttgtaataaa gaaacagcaa aataaaagta caaatggcac cataccataa gaaatcataa
     1981 gaacacgcag gaagccaagc ttttttgggt tttgaaattt tttagggatt ggatttccgt
     2041 ctgcgtcttt agtgatcagt tttggttctt tttttgactt tttctttgcc ataattcaat
     2101 ccttttaatg tgaaatattg cttttcatat tataatatat aagcatgata aacgctttta
     2161 aatttgtaag ggagaaaaaa tgaatttatc aaatatagta attccagttg ttcttgctgc
     2221 agtcttaact gttgtcacct ttgtacaatt aagaaagaaa gacgaatata ctacgggcaa
     2281 ga
//
LOCUS       DBNCDC010000123         2264 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_42710_length_2264_cov_6.100498, whole genome shotgun sequence.
ACCESSION   DBNCDC010000123 DBNCDC010000000
VERSION     DBNCDC010000123.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42710_length_2264_cov_6.100498"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="AC04VQ_07175"
     CDS             <1..733
                     /locus_tag="AC04VQ_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIO9553875.1"
                     /translation="HVSTPVFDGANEDEIQFAIQAANKNLVKIHNKKYGKHADESMVP
                     QLSETGKVWLYDGRTGEKFREPITVGQAYILKLGHMVDDKIHARSIGPYSLITQQPLG
                     GKAQFGGQRFGEMEVWALYAYGASNVLQEILTIKSDDTSGRVKSYEAIVKGEELPKAE
                     VPESFKVLIKEMKSLALNVELRGEAKDEAKDDKSVEEALLEGLEKDAQEEDLDLSKEL
                     EDIEASLELEDVDTSDDKGEEKGDK"
     gene            733..>2264
                     /locus_tag="AC04VQ_07180"
     CDS             733..>2264
                     /locus_tag="AC04VQ_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIO9553876.1"
                     /translation="MATEYDVNNFDELKISLASADDIRSWTSGEVKKPETINYRTLKP
                     EKDGLFCEKIFGPTKDWECACGKYRRVRFKGIICERCGVEVTRSKVRRERMGHIELAA
                     PVSHIWYFKGVPSRLGYFLDITTKDLEKVLYFASYMITWVDTEAREEDKEELEEELKE
                     DLEELDAECEKIIEDTKKLSVDYVPEEDEIVDEAFEEERLTAEEIEQEIIDTKEEFAE
                     AKQLRKDAYKEFMKIKPKELIEDENVYREMRANYRDYFRGGMGAEVVKDLLEQVNLQE
                     LHDELDEIIKTGKGPKREKAIKRIRIVNAFIESKTNKPSDMILECIPVIPPDLRPMVQ
                     LDGGRFATSDLNDLYRRVINRNNRLRRLQELGAPEIIVNNEKRMLQEAVDSLFDNGRR
                     GRFITGPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPTLKLHQCGLPT
                     EMALELFKPFVMRRLVELDYVANIKVAKRAVETGPDYVFDVLEEVVAEHPVLLNRAPT
                     LHR"
BASE COUNT          675 a          397 c          557 g          635 t
ORIGIN      
        1 tcatgtttca actcctgttt ttgatggtgc taacgaagac gaaattcagt tcgctattca
       61 agcagccaac aaaaacttgg taaagattca taacaagaaa tatggcaaac atgcagatga
      121 aagcatggtt ccacagctaa gcgaaactgg taaggtttgg ctttatgatg gtcgcacggg
      181 cgagaagttc cgcgaaccaa ttactgtagg acaagcttat atcttgaagt tgggccacat
      241 ggttgatgat aagattcacg ctcgttcgat tggaccttac agcttgatta ctcagcaacc
      301 tcttggtggt aaggcacagt ttggtggcca gcgcttcgga gagatggaag tttgggcact
      361 ttatgcttat ggtgcatcga atgttttaca agaaattttg actatcaagt ctgatgatac
      421 ttctggtcgt gtaaaaagtt acgaggctat tgttaaaggc gaagagcttc caaaagcaga
      481 agttcctgaa tcattcaagg ttttgattaa ggaaatgaag tcattggcgc ttaatgttga
      541 gcttcgcgga gaagcaaaag acgaggcaaa ggatgacaaa tctgtcgaag aagcactttt
      601 agaaggtctt gagaaggatg ctcaagaaga agatcttgat ttgagcaaag agctcgaaga
      661 cattgaagcc tcacttgaat tggaggatgt agacacctca gatgataaag gcgaagagaa
      721 aggagataaa taatggctac agaatacgat gtaaataatt ttgatgaatt aaaaatctcc
      781 cttgctagcg ccgatgatat tcgtagttgg acttctggcg aagttaagaa accagaaaca
      841 atcaactaca gaactctgaa gcctgaaaaa gacggacttt tctgcgagaa gatttttggt
      901 cctacaaagg attgggaatg tgcttgcggt aaatatcgac gcgttcgttt taagggcatt
      961 atttgtgagc gatgcggtgt tgaggttact cgttctaagg ttcgccgtga gcgcatgggt
     1021 catattgaac ttgctgctcc agtaagccat atttggtatt ttaagggcgt tccttcacgc
     1081 ttgggttact tccttgacat tacaactaag gatctggaga aggtcttgta cttcgccagc
     1141 tatatgatca catgggttga tacagaggct cgcgaagaag acaaagaaga acttgaagag
     1201 gaacttaaag aggacttaga agagcttgat gcagaatgcg aaaagattat cgaagatacc
     1261 aaaaagcttt ctgttgatta tgttcctgaa gaagacgaaa ttgttgatga ggctttcgaa
     1321 gaagagcgct tgactgctga agaaattgag caggaaatta tcgacaccaa agaagagttt
     1381 gctgaagcaa aacaacttcg taaagatgcc tataaagaat ttatgaaaat taagcctaag
     1441 gaattaatcg aagatgagaa tgtttaccgc gaaatgcgcg caaactatcg tgattacttc
     1501 cgtggaggca tgggtgctga agttgtaaaa gacttgcttg agcaggttaa tttgcaagaa
     1561 cttcacgatg aattggatga gattatcaaa actggtaagg gtccaaagcg tgaaaaagct
     1621 atcaagagaa ttcgcattgt taatgctttc attgaaagca agactaacaa gccatctgat
     1681 atgattttgg aatgtatccc ggttattcca ccggatttgc gtccaatggt tcagttggat
     1741 ggtggccgtt ttgctacttc ggatctaaac gatttgtatc gtcgtgtaat taacaggaac
     1801 aaccgtcttc gtcgtttgca agaacttggt gctcctgaga ttattgttaa caacgaaaag
     1861 cgtatgcttc aagaggcagt tgacagtttg tttgacaatg gtcgccgtgg acgtttcatt
     1921 acaggtcctg gtaaccgtcc tcttaaatcg ctttctgaca tgctaaaagg taagcaaggt
     1981 cgtttccgtc aaaacttact tggtaagcgt gttgattatt ctggtcgttc tgtaattgtt
     2041 gttgggccaa cacttaaact tcatcagtgt ggtcttccaa ccgagatggc gcttgagctt
     2101 ttcaagccat ttgtaatgcg ccgcttggtt gagcttgact acgttgcaaa tattaaagtt
     2161 gcaaagcgtg cggttgaaac tggtcctgac tacgtcttcg atgttttgga agaggtagtt
     2221 gcagaacatc cagttttgct aaaccgtgct cctacgcttc accg
//
LOCUS       DBNCDC010000124         2209 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_44510_length_2209_cov_5.665274, whole genome shotgun sequence.
ACCESSION   DBNCDC010000124 DBNCDC010000000
VERSION     DBNCDC010000124.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44510_length_2209_cov_5.665274"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="AC04VQ_07185"
     CDS             <1..284
                     /locus_tag="AC04VQ_07185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIO9553877.1"
                     /translation="IRAIHQENGGHGQAVNTGLKNAKGLYFKVVDSDDWLDVDGTKSI
                     MDYIRKQAKGQASKATDLVIGNYVYNKVSEGTQTPIDYCKALPEGREFN"
     gene            324..707
                     /locus_tag="AC04VQ_07190"
     CDS             324..707
                     /locus_tag="AC04VQ_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553878.1"
                     /translation="MHAVVYKTSVLRDMKLKLPKHTFYVDNIFVYVPLPHVKSAYYIN
                     SNMYMYYIGREDQSVNEDVMKSRIDQQLKITKIMIDATDLPALDKQPKLERYMLNYLS
                     MMMCICSVFLRMIGTAEAEVKRKEI"
     gene            1010..2155
                     /locus_tag="AC04VQ_07195"
     CDS             1010..2155
                     /locus_tag="AC04VQ_07195"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIO9553879.1"
                     /translation="MLKTVWLSNIIEKDLATKDSPLRASIDDLIEIYGSDGLTFVVYE
                     PNGVFSLSCYVDQNDAYILEPTNNQYRDNTYKHFLNSEVPLIDGLDLCLPLKTARTKV
                     FGFVVLHGSGQGEAVALSNIELVALDNFASLLHSEAIGSIAKSYYGTVIDIKDLCVEY
                     KTGQNVTVAVNNLNLEICEKEFSLILGASGCGKTSLLNAIGGMLHPKSGSVIYNGQDI
                     TKFRGAKATNYRKETVGFIFQQYNLISDLTAKENIEVASSLVEDKYSAEEVLSLVGLK
                     EKINNYPGQMSGGEQQRVCIARALVKKPKLLLCDEPTGALDTNNAISVMKILQDLVKK
                     QNVPVVMITHNSEFASLADHYVLMSNGKIVEEYRQPFAMEAKNLVIR"
BASE COUNT          695 a          352 c          462 g          700 t
ORIGIN      
        1 tgattcgtgc cattcatcaa gaaaatggtg gacacggaca agctgtaaat actggcttga
       61 aaaatgctaa gggtctatat tttaaagttg ttgatagcga tgattggctt gacgttgatg
      121 gcacaaagtc aataatggat tatattcgta agcaggctaa aggccaggct tcaaaggcaa
      181 ccgatcttgt cattggaaat tatgtttata acaaagtttc cgaaggcact caaacaccaa
      241 ttgattattg caaggccctt cctgaaggtc gtgagtttaa ttgaagtgag attcgcaagt
      301 ttaagctaag cgaatatttg cttatgcatg ctgttgttta taaaacttct gtccttcgtg
      361 atatgaaatt aaaacttcca aagcatactt tttatgttga taatattttt gtgtatgttc
      421 cgctgccaca tgttaaaagc gcttattaca tcaattcgaa tatgtatatg tattacattg
      481 gcagggaaga tcaaagtgta aacgaagatg ttatgaaatc tcgaattgat cagcagctta
      541 aaattacgaa gattatgatc gatgcgacag atttacctgc gcttgacaag cagcctaagc
      601 ttgagcggta tatgctaaat tatctttcta tgatgatgtg catttgtagc gtatttcttc
      661 gtatgattgg caccgccgaa gccgaggtca agcgtaagga aatttgacaa tatgctaaaa
      721 accatgacga agccttatat aagaaattaa tgaaatcacc actttgttat ggcacaaatg
      781 ttccgggaaa attcggaagg tttgttggcc taacaggata taaactagct caaaagctat
      841 ttggctttaa ctaagatata aaattccttg tttttgtggt atattatgcc acctggcaca
      901 cctccttggt gtaatggtag cacaccggtc ttcaaaaccg ggttgtctcg tgtccaaagc
      961 gagcggtggg ggttcgaatc ctccaggagg tgccattttt aggtgattta tgttaaaaac
     1021 tgtatggtta tcaaatatta ttgagaagga tttagctacc aaagatagcc ctttgcgagc
     1081 ttcgattgat gatcttattg aaatttatgg atctgatgga ctaacatttg ttgtttatga
     1141 accaaatggg gtttttagtc tttcatgtta tgtcgaccaa aatgatgctt atattcttga
     1201 acctacaaat aatcaatata gagataatac atataagcat tttttaaata gcgaagttcc
     1261 attaattgat ggtcttgatt tgtgcctgcc tttaaagaca gctagaacaa aagtttttgg
     1321 ttttgttgtt ctccatgggt cgggtcaggg cgaagccgtc gcgctcagca atattgagct
     1381 ggtggcgtta gacaattttg catcgttatt gcactcagaa gcaataggaa gtattgcaaa
     1441 atcttattac ggcacagtaa ttgacattaa agatttgtgc gttgaataca aaactggtca
     1501 aaatgttacc gttgctgtta ataacttaaa cctagaaatc tgcgaaaaag aatttagctt
     1561 gattttaggc gcgagcggtt gcggtaaaac ttcgttactt aacgctattg gaggaatgct
     1621 tcatccaaaa agtggttctg ttatctacaa cgggcaggac attactaaat ttcgtggtgc
     1681 taaagctact aattatagga aggaaactgt tgggttcatt ttccaacaat acaatttaat
     1741 ctcagattta acagctaagg aaaatatcga ggttgcttca tcgttggttg aagataaata
     1801 ttcagctgaa gaagttttaa gtcttgttgg cctaaaggaa aagatcaata attatccagg
     1861 tcagatgagt ggtggcgaac aacaaagagt ctgcattgca agagcactag ttaaaaagcc
     1921 gaagctttta ttgtgtgatg aacctactgg tgcgcttgat acaaataatg caattagcgt
     1981 tatgaagatc ttgcaagacc ttgttaaaaa acaaaatgtt ccagttgtta tgattactca
     2041 taattcagaa tttgcttctt tggcagatca ttatgttttg atgagtaatg gaaaaatcgt
     2101 tgaggaatat agacaacctt tcgcaatgga agcaaagaat cttgtaattc gataggcaat
     2161 tctttatgaa aaacactctt acattaagtt tttcaatttt caaagcaaa
//
LOCUS       DBNCDC010000125         2208 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_44548_length_2208_cov_6.170460, whole genome shotgun sequence.
ACCESSION   DBNCDC010000125 DBNCDC010000000
VERSION     DBNCDC010000125.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44548_length_2208_cov_6.170460"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="AC04VQ_07200"
     CDS             <1..719
                     /locus_tag="AC04VQ_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013978542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIO9553880.1"
                     /translation="SLPGKLADCSSKEAEKSEIFIVEGDSAGGSAKQARDRQFQAIMP
                     LRGKILNVERAGLSRSLSSDTISSLITAIGTGIGEDFDADNARYHRIIIMTDADVDGA
                     HIRTLLLTFFYRYMPELIQRGYIYIAQPPIFGLKKKNSKSTKVERYIYDENALSDTLK
                     EYDDGSKFDVQRYKGLGEMDPEQLWETTMAPDQRTLLQVTIDDAAEAERVVSELMGDA
                     VEPRKEFIQSHAKDVRFLDI"
     gene            735..>2208
                     /locus_tag="AC04VQ_07205"
     CDS             735..>2208
                     /locus_tag="AC04VQ_07205"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing)"
                     /protein_id="HIO9553881.1"
                     /translation="MADENKKEIKQPKEEKATTEEQKAQQMMIDMPEAHGDVVEANGG
                     DGTTVRATFLGKEMQTSFLEYAMSVIVARALPDVRDGLKPVHRRILFAMEDAGFTPNK
                     PHMKSARTVGDVIGKYHPHGDVAVYDTMVRLAQPFSLRVPLIDGHGNFGSIDGDSPAA
                     MRYTEARLDKAAMEMLRDLDKDTVDFVPNYDESLEEPVVLPARFPNLLVNGSNGIAVG
                     MATNIPPHNLGETIDAVSLLLDNPDATTNDLLKVMPGPDFPTGGQIMGRKGIHDYYET
                     GRGSLQIRAKCEIEERKGGKSAIIVKEIPYQVNRQRLLEKLGELVRDKKLPEISNIHD
                     GADRHGIDIIIELKNSAVPEVVLNKLYKQTQMQVSFGVIMLALVDGVPKVLPLKSMLN
                     HYIEHQKEVITRRTKFELDKAEKRAHILEGYVIALDNIDEVIKIIRGSQTDKEAAEKL
                     TKKFGLSKAQTDAILEMKLRRLTGLERDKIEAELKELRELI"
BASE COUNT          648 a          393 c          524 g          643 t
ORIGIN      
        1 ttagtttgcc tggaaagttg gcagattgtt caagcaagga agctgagaaa agtgaaattt
       61 ttattgttga gggtgacagc gcgggtggtt ctgctaagca agctcgtgac cgtcaattcc
      121 aggcaatcat gcctcttcgt ggtaagattt taaatgtaga gcgtgctgga ctttcgcgct
      181 ccctttcaag cgacacaatt tcttcgttga ttacagcaat tggaactgga atcggcgaag
      241 attttgatgc ggacaatgca agataccacc gaatcattat tatgactgat gctgatgttg
      301 atggtgctca cattcgcact ttgcttctaa catttttcta cagatatatg cctgaactta
      361 ttcagcgagg ctacatctat attgcgcagc ctccaatttt tgggttgaag aagaaaaatt
      421 cgaagagcac gaaagttgag cgctacattt atgatgaaaa tgcgctttct gatacgctca
      481 aagaatatga cgatggttca aaatttgacg ttcaacgtta taagggtctt ggtgagatgg
      541 atcctgagca gctttgggag accacaatgg ctcctgatca gcgtacactt ttgcaagtta
      601 caattgatga tgccgccgaa gctgagcgcg ttgtaagtga attgatggga gatgcggttg
      661 agcctcgtaa agaatttatt caatcccacg ctaaagatgt tcgattctta gatatttagg
      721 aaatggtgat ataaatggca gatgaaaaca aaaaagaaat aaaacaacct aaagaagaaa
      781 aggctactac cgaagagcag aaggcacaac aaatgatgat agatatgcct gaagctcatg
      841 gtgatgttgt tgaggctaat ggaggagatg gcacaacggt ccgtgcgact tttcttggca
      901 aagaaatgca aaccagtttt ttggaatatg cgatgagcgt tattgttgct cgtgccttgc
      961 ctgatgttcg cgatggctta aagcctgttc atcgtcgtat tttatttgcg atggaagatg
     1021 ctggctttac tccaaataag cctcatatga aatcagctcg aacagtaggt gatgtaattg
     1081 gtaagtatca ccctcatggt gacgttgctg tttatgatac gatggttcgc cttgctcaac
     1141 ctttcagttt gcgtgttcct ctaattgatg gccacggaaa cttcggaagt attgatggtg
     1201 acagtccagc agctatgcgt tatacagaag cgcgacttga caaagctgcg atggaaatgc
     1261 ttcgtgattt agataaggat actgttgatt ttgttcctaa ctatgatgaa agtttggaag
     1321 aaccagttgt tttgccggcg cgtttcccga atttgttggt caatggttca aacggtattg
     1381 ctgttggtat ggcaacaaat attccgcctc ataatttagg ggagactatt gatgctgtat
     1441 cacttttgct tgataatccg gacgctacaa ctaatgattt gctcaaggtt atgcctggtc
     1501 cagatttccc gactggtggt caaatcatgg gacgtaaagg cattcatgat tattatgaaa
     1561 caggccgagg ctcacttcaa attcgtgcta agtgtgaaat tgaagaacgc aaaggtggca
     1621 agagcgccat tattgtaaaa gagattcctt atcaagttaa cagacaacgt ttgcttgaga
     1681 agttaggtga gcttgttcgc gataaaaaac ttcctgaaat ttcaaacatt cacgatggtg
     1741 ctgacagaca tggaattgat attattattg aacttaaaaa ttctgctgtt cctgaagttg
     1801 tacttaataa actttacaag caaactcaaa tgcaagtttc ctttggtgtt attatgcttg
     1861 ctttggttga tggcgtgcca aaggttctac ctcttaagag tatgcttaac cattatattg
     1921 agcaccaaaa agaggttatt actcgtcgca caaaattcga gttagataag gcagaaaagc
     1981 gcgctcacat cttagaaggt tatgtaattg cacttgataa tattgacgaa gttatcaaaa
     2041 ttattcgcgg cagccaaacc gataaagagg cagcggaaaa attaacaaag aagtttgggc
     2101 tttcgaaggc acagaccgat gcaatcttgg agatgaaact tcgtcgtttg accggacttg
     2161 aacgtgacaa gatcgaagct gagttgaaag aacttcgtga attgattg
//
LOCUS       DBNCDC010000126         2113 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_48072_length_2113_cov_5.819242, whole genome shotgun sequence.
ACCESSION   DBNCDC010000126 DBNCDC010000000
VERSION     DBNCDC010000126.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48072_length_2113_cov_5.819242"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1705
                     /locus_tag="AC04VQ_07210"
     CDS             <1..1705
                     /locus_tag="AC04VQ_07210"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: protein motif:HMM:TIGR01427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HIO9553882.1"
                     /translation="NWDSLDGEPVKLAILIAAPQTSDNLHLDVLAKLSGMLMHDDFRE
                     GLLNAKDEAEFYQRIVDADKEEEEKAAEDNSNINPEGPLILAVTACPTGVAHTYMAKE
                     SLETKAKELNINLKVETDGSGGIKNELTEEDIKKAKAIIVAADAFVQMGRFNGKPVIE
                     VPVSKAIGSPDVLLNSAVNDNIPKYKAGASEVFQIGAGDGRMEGSSKAHNVYKHLMSG
                     ISHMIPFVVAGGILLAFAYMIDGLCGSPKDGLEIDGIYYAFGSINEAARVLHDLGATF
                     GLGLMLPVLGGFIAYSIAGKPGLVSGFVGSFAATQGMYSALYYYSLLVYGIEAESTIQ
                     LATSSAGFIGAIAAGFIAGYFVKFLQRKFSNFSRTLEPVRDMLIIPLISTAFVGACMF
                     FLNAPFGLLNIYLGIGLQVLKDANLILLLAALVSALMATDMGGPINKATHYFVLSLVT
                     TSLGSSGTPEMQTLACQLMAANIVGIMVPPVGIAIATWLFPQKFTAADRVPSLANFFI
                     GLSGITEGAIPYVAISPVIFIFCCMTGAAVGGVISMLLGMEAIAPEGGTISYLVMGAS
                     C"
     gene            1885..1961
                     /locus_tag="AC04VQ_07215"
     tRNA            1885..1961
                     /locus_tag="AC04VQ_07215"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1919..1921,aa:Glu,seq:ctc)
BASE COUNT          530 a          412 c          502 g          669 t
ORIGIN      
        1 taactgggat tcgcttgatg gcgaacctgt taagttggcg attttgattg ctgcacctca
       61 aacgtctgat aatttgcact tggatgtttt ggcaaagctt tctggaatgc tgatgcatga
      121 tgattttcgc gaaggcttac tcaacgcaaa agacgaagct gaattctatc aacgaattgt
      181 tgatgctgac aaggaagagg aagaaaaagc tgccgaagac aattccaaca tcaacccaga
      241 aggaccatta attttagctg taacagcttg tcctactggc gttgcacata catatatggc
      301 taaggaatca cttgagacta aggctaagga acttaacatt aacctcaagg ttgaaactga
      361 tggctctggc ggtattaaaa atgagttgac cgaggaagac atcaagaaag caaaagcaat
      421 tattgttgct gcagatgctt ttgtccaaat gggtcgattc aacggcaagc cagtcattga
      481 agttccagtt tcaaaggcga ttggctctcc tgatgtttta ttaaattctg ccgtaaatga
      541 taatattcca aaatataagg cgggggcttc ggaggtattt caaattggtg ccggcgatgg
      601 cagaatggaa ggctcaagta aagcccataa tgtttacaag cacttgatgt ctggaatttc
      661 tcacatgatt ccgttcgttg ttgctggtgg tattttgctt gccttcgcat atatgattga
      721 tggtctttgc ggatcgccta aagatggtct tgaaatcgac ggcatatatt acgcttttgg
      781 ttcaattaat gaagctgcaa gagtattgca tgatttaggt gcgacttttg gtcttggatt
      841 gatgctgcct gttcttggtg gttttattgc gtattcaatt gcaggtaaac ctggacttgt
      901 ttctggattt gttggaagtt ttgcagcaac gcaaggtatg tattcggcgc tttattatta
      961 ttcgctattg gtttatggta ttgaagcaga aagcacaata cagcttgcaa cttcaagcgc
     1021 tggttttatt ggagcaattg cagctggctt tatcgcaggt tattttgtca agtttttgca
     1081 aaggaagttt tcgaatttca gtagaacgct tgagcccgtg cgcgatatgt tgataatccc
     1141 acttatttca acagcttttg taggtgcatg tatgtttttc ttaaatgcgc catttggctt
     1201 gcttaatatt tatcttggaa ttggactcca agtattgaaa gatgcaaatt tgattttgct
     1261 tcttgctgct cttgtttctg ctttgatggc aaccgatatg ggaggcccaa taaacaaggc
     1321 aactcattac ttcgtgctaa gtcttgttac aacatctctt gggtcaagcg gaactccaga
     1381 aatgcaaacg cttgcttgtc agttgatggc cgcaaacatc gttggaatta tggttcctcc
     1441 agttggcatt gcaattgcaa cttggctttt ccctcaaaag ttcacagcag ctgacagagt
     1501 gccttcgctt gcaaacttct tcattggact ctctggcatt accgaaggtg caattccgta
     1561 tgttgcaatt agtcctgtaa tatttatttt ctgttgcatg acaggtgctg ctgttggtgg
     1621 cgtaataagc atgcttttgg gtatggaagc tatcgctccc gaaggtggaa caatttccta
     1681 tcttgtaatg ggtgcttcct gttgaaaggg tatagttgcg accttcgcag gtgcttttgt
     1741 aacagcaatt ttgattggtt tgctccgaaa agatgtcgag ccgcaatatg ctaagttagg
     1801 caagtggaaa ggcattccaa tcggtcgtaa atttaaataa ttgaaacgct tgttgttgtg
     1861 ctaaaattac cgcttgctta aagtggcccg gtcgtctagc ggtttaggac gccgccctct
     1921 caaggcggag gtcgccggtt cgaatccggt ccgggctacc atttaggatg cttggcctga
     1981 cggccttcgc atcaagcttt cgctccgaaa taattctatc catcatctgc acccttgttt
     2041 tttgactcgc gtacgcagta cgcgtcgtca aaaaggcggg cacatctgac ggatacaatt
     2101 atttctcgct gtc
//
LOCUS       DBNCDC010000127         2070 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_49813_length_2070_cov_7.021340, whole genome shotgun sequence.
ACCESSION   DBNCDC010000127 DBNCDC010000000
VERSION     DBNCDC010000127.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49813_length_2070_cov_7.021340"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            86..1186
                     /gene="dnaN"
                     /locus_tag="AC04VQ_07220"
     CDS             86..1186
                     /gene="dnaN"
                     /locus_tag="AC04VQ_07220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIO9553883.1"
                     /translation="MKFSINQSALNDILNTVNKAVSQRATNPALAGILLETNENEVTL
                     RGTDLELSIQATAPALIEEDGKALLPAKLLSDIVGNLPDEAVNIEVAEDKADITCANS
                     NFSIRTLNATDYPEFPKVETKEKVTLPFKEFSDMTKRVAKMTARDDSRPILQGVNVKV
                     NGNKLELVATDAYRIAVASAEVETDNTSEFTAVLPGQFMMDVAGLKGDIDKATISLSE
                     NQVVVECNDMIFINRRIAGTFPPHDQFLSAEPKLVATVNVKELTEAVRRISVLSYIAQ
                     AIKMTFDPGTNKLALSGTAADVGSCDEIVEAKYSTDCEKIDISFNCAYVLDGLTCFNG
                     DEVNIAIVAGDKPGVFKDDGHIHYVLLPVKVD"
     gene            1193..>2070
                     /gene="recF"
                     /locus_tag="AC04VQ_07225"
     CDS             1193..>2070
                     /gene="recF"
                     /locus_tag="AC04VQ_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.2"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIO9553884.1"
                     /translation="MPVGFTINKISLRNFRNYENHTRDFSSGVNKIVGPNATGKTNII
                     EAIGLMTQISSFRNAQILELVNKNHADEVTQIEVEIFDMATETKNDFTLKIQDGKKTY
                     KLNGKTKTISDCKGRFPSVIFTPDDLALVKGSNTMRRAAVDSLGLQISPDYYRVLHDY
                     NKALKQKNAMLRDGASLDMLKSINDVLVMSGAQLVFYRLGLVQKILPVVSNYYDEISG
                     GNENLNVEYYPSWSMDDELREISRDEARELLAKAFDEKTAEEQARCRALIGPHRDRIV
                     FTLNESDAQSFASQGQ"
BASE COUNT          586 a          475 c          479 g          530 t
ORIGIN      
        1 caaaattcat ccaacaaaag caataaatat taataataaa atgtagtgat ttgctataat
       61 gcgagcagac gaagaaaggc gaattatgaa attttcaatt aaccaatcag cattgaatga
      121 cattttaaac actgttaata aggcggtttc gcagcgtgcc acaaaccctg ctcttgcagg
      181 cattttgtta gagacgaacg aaaacgaagt cacgcttcgt ggcaccgatc ttgagctttc
      241 gattcaagcg accgcgccgg ctctcatcga ggaagatggc aaggcgctcc ttcctgcaaa
      301 gctgctctct gacattgttg gcaaccttcc ggacgaggcc gtgaacatcg aagttgccga
      361 agacaaggcc gacatcactt gcgcgaattc caacttcagc attcgcacct tgaacgcaac
      421 cgattatcct gagttcccga aggtcgaaac caaggaaaaa gtcactttgc cgttcaagga
      481 attttcggac atgacaaagc gtgtcgccaa gatgaccgcg cgcgatgatt cgcgtccaat
      541 tttgcaaggc gtcaatgtta aggtgaacgg aaacaagctt gagctggttg caaccgatgc
      601 ttacagaatt gctgtagcaa gtgccgaagt cgagaccgac aatacttcgg agtttaccgc
      661 cgttcttccc ggccaattta tgatggatgt agctgggctt aagggcgata tcgacaaagc
      721 cacaatttcg ctttcggaaa accaagttgt tgttgaatgc aacgatatga tttttatcaa
      781 ccgccgaatt gctggcacct tccctccgca cgaccagttc ttgagcgcgg agccgaagct
      841 tgtcgccacc gttaatgtta aggagctgac cgaagccgtc cgccgcatca gcgttttgtc
      901 atacattgcg caggccatca agatgacttt tgacccgggc acaaacaagc tcgcgcttag
      961 cgggactgct gccgatgttg gttcttgcga tgaaattgtc gaggcgaagt attcgaccga
     1021 ttgcgagaaa attgacattt cgtttaattg cgcttacgtg cttgacgggc ttacgtgctt
     1081 taatggtgac gaagtaaata tcgccattgt agcaggtgat aagccaggtg ttttcaagga
     1141 cgacggccac attcattacg ttttgcttcc tgtaaaggtt gactaaaacg caatgcctgt
     1201 tggttttaca atcaataaaa taagtctgcg aaattttcgc aattatgaaa atcacacgcg
     1261 cgattttagt tcgggcgtga acaaaattgt cggcccgaac gctaccggca agacaaatat
     1321 catcgaagcg attggcctca tgactcaaat ttcgagtttc cgcaacgctc aaattcttga
     1381 acttgtgaat aaaaatcatg ctgacgaagt tacgcaaatc gaagttgaaa tttttgatat
     1441 ggcaaccgaa acgaaaaatg attttacgct aaaaattcaa gatggcaaaa aaacttacaa
     1501 gttaaacggc aagaccaaaa caatttcgga ttgcaagggt cgcttcccga gcgttatttt
     1561 tacccccgat gacctggcac ttgtcaaagg ttctaacact atgcgtcgcg ctgcggtcga
     1621 tagcctcggc cttcaaattt cgccggacta ttaccgtgtt ttgcacgatt acaacaaggc
     1681 gctcaagcaa aaaaatgcga tgttgcgtga cggtgcgagc ctcgatatgc taaaaagcat
     1741 caacgatgtt ctggttatgt ctggcgcaca acttgttttt tatcgccttg gtttggttca
     1801 gaaaattttg cctgtcgttt caaattatta cgatgaaatt tccggcggca atgaaaatct
     1861 caacgttgaa tattatcctt cgtggtcgat ggatgatgag cttcgcgaaa ttagccgtga
     1921 cgaagctcgc gaacttcttg ctaaagcgtt tgatgaaaaa accgccgaag agcaggctcg
     1981 atgccgagct ttaattggtc cacaccgcga taggatcgtt ttcaccttaa acgaaagtga
     2041 cgcccagagc ttcgccagtc aaggtcaaca
//
LOCUS       DBNCDC010000128         2061 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_50178_length_2061_cov_5.003490, whole genome shotgun sequence.
ACCESSION   DBNCDC010000128 DBNCDC010000000
VERSION     DBNCDC010000128.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50178_length_2061_cov_5.003490"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            39..287
                     /locus_tag="AC04VQ_07230"
     CDS             39..287
                     /locus_tag="AC04VQ_07230"
                     /inference="COORDINATES: protein motif:HMM:NF026064.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553885.1"
                     /translation="MPTNKLNNWLMGIKETGFSVTKGKKVLRLKYMTQTGDRPPQFSI
                     FVNHVSIVNDNFERFFENRLRENFDLSGTPITFRFKTK"
     gene            297..1055
                     /locus_tag="AC04VQ_07235"
     CDS             297..1055
                     /locus_tag="AC04VQ_07235"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HIO9553886.1"
                     /translation="MEENKNQNGQPQQKKKAKIIAIVAASDFNEKHFMKSLQGGMFAS
                     IIAADAGYRHLDTRGIVPNVAIGDFDSLGYVPKEDKQASVQLKEDVDAGTPKNIRVMQ
                     YPEDKDKTDLELAIDEALNMKPQILMIYGALGKRLDQSLSSIQMLSHIADHRVGVFCV
                     GDKEACAVVNGPSEFGLGIPNDDSLDKTISIFSLTDVSLVEASQGLKWPYTNTAITNS
                     MSLGVSNEITANPMKIKVKMGKILLVFSSELLGK"
     gene            1065..1436
                     /locus_tag="AC04VQ_07240"
     CDS             1065..1436
                     /locus_tag="AC04VQ_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553887.1"
                     /translation="MSEKVDVFRYKASEETVQKLRALAPKYSRLNRDFSLTKQDCIEG
                     KNLSDNINNAESNLELKTYDGFKVYVLGEKNSDEAILYLHGGGYLFGLYKNQVGIMDK
                     FYQETGKQVYIVDYGLPVKYN"
     gene            1452..1958
                     /locus_tag="AC04VQ_07245"
     CDS             1452..1958
                     /locus_tag="AC04VQ_07245"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HIO9553888.1"
                     /translation="MLEKVYEEIISNNKNFILIGDSAGAGMGLGFIQLLNQRGQKTPR
                     KQVLISPWLDITMSNPEVKNYEEKDAFLAVDCLIGAGELWANGLDRKDPRLSPIFGDL
                     TNIPETMLTTGTCEVMYPDIIKLSEELTNNGTKVHLIICEGLYHAYPYFPIEDSKLTL
                     QKIIDFIR"
     gene            1986..2060
                     /locus_tag="AC04VQ_07250"
     tRNA            1986..2060
                     /locus_tag="AC04VQ_07250"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2018..2020,aa:Asn,seq:gtt)
BASE COUNT          676 a          330 c          430 g          625 t
ORIGIN      
        1 acgtttatag aaattggtgg ttggttatta tataaaaaat tcctaccaat aaacttaata
       61 attggttgat gggaattaaa gagactggct ttagcgttac aaaaggcaaa aaggtgcttc
      121 gtttgaaata tatgactcaa actggcgata ggcctccgca atttagcatt tttgtaaatc
      181 atgttagcat tgtaaatgac aattttgagc gctttttcga aaatagattg cgtgaaaatt
      241 ttgacttaag cggtacccca ataactttta gatttaagac aaagtaggtg tttaaaatgg
      301 aagagaacaa aaatcaaaat ggtcagcctc agcaaaaaaa gaaagccaag ataattgcta
      361 ttgtagctgc ttctgatttc aatgagaaac attttatgaa aagtttgcag ggcggtatgt
      421 ttgcaagtat tattgcagct gatgccggtt acagacattt ggacacccgt ggtattgttc
      481 caaatgttgc aatcggcgat ttcgatagtc ttggctatgt ccctaaagag gataaacagg
      541 cgagcgttca gcttaaagaa gatgttgatg cgggcactcc gaagaacatt cgtgttatgc
      601 aataccccga agacaaggac aagactgacc ttgaacttgc aatcgacgaa gcgcttaaca
      661 tgaagccgca aattcttatg atttatggcg cgcttggcaa gcgtcttgat caatctttgt
      721 cttctattca aatgttaagt catattgctg atcatcgtgt tggcgtattt tgcgttggtg
      781 ataaagaagc gtgcgcagtt gttaacgggc cttcggaatt tggacttgga attccaaatg
      841 atgatagcct tgataaaaca atttcaattt tctcgctaac tgatgtgtct cttgttgaag
      901 cttcgcaagg acttaagtgg ccatacacaa atacggcaat aacaaatagt atgtcgcttg
      961 gtgtatccaa tgaaattact gctaacccta tgaaaataaa agtgaagatg ggcaaaatct
     1021 tgcttgtatt ttcatcagaa ttgttaggta aataaggagg aattatgtca gaaaaggttg
     1081 atgtctttcg ctacaaggct tcagaagaaa ctgttcaaaa actaagagct cttgctccaa
     1141 aatattcaag attaaataga gatttttctt taacaaaaca ggactgtatt gaaggaaaga
     1201 accttagtga caatatcaat aatgccgaaa gcaatcttga acttaagacc tatgatggtt
     1261 ttaaagttta tgtattaggc gaaaaaaatt ctgatgaagc aatattatat ttacacggtg
     1321 gcggatattt attcggtctt tataaaaacc aagttgggat aatggataaa ttttatcaag
     1381 aaactggaaa gcaagtttat atagtcgatt atggtctacc ggtgaaatat aattgagaac
     1441 atgcttatac cttgcttgaa aaagtctatg aagaaattat tagcaataac aaaaatttta
     1501 tcctaatagg tgactctgct ggggcaggca tgggtttagg ttttattcaa cttttaaatc
     1561 aacgaggtca aaaaactcct agaaagcaag ttttaatttc accttggctt gatataacaa
     1621 tgagtaatcc agaagttaag aattacgaag agaaagatgc ttttcttgca gttgattgcc
     1681 taattggcgc aggtgaatta tgggctaatg gattggacag aaaggatcct cgtttaagtc
     1741 ctatctttgg tgacttaaca aatattcccg aaaccatgct tacaactggg acttgcgagg
     1801 ttatgtatcc agatattatc aagcttagcg aagaactcac aaacaatggt acaaaagttc
     1861 atttgataat ttgcgaagga ctttaccacg cttatccata ttttccaatt gaggattcaa
     1921 agctaactct tcaaaaaatt atagatttta ttagatagtt ttgggctata atgctcactc
     1981 gacgttcctc ggtagctcaa tggcagagca ttcggctgtt aaccgaaggg ttgtaggttc
     2041 gagtcctacc cggggagcca t
//
LOCUS       DBNCDC010000129         1856 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_60308_length_1856_cov_9.022210, whole genome shotgun sequence.
ACCESSION   DBNCDC010000129 DBNCDC010000000
VERSION     DBNCDC010000129.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60308_length_1856_cov_9.022210"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(130..420)
                     /locus_tag="AC04VQ_07255"
     CDS             complement(130..420)
                     /locus_tag="AC04VQ_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553889.1"
                     /translation="MNKEAIQAILAKHLNEKKKIEDLHLNGNNLLGYVKEDEYSIDKY
                     SPVEVQYEEDYLVLKGHRYSYNKNHRKVTEDYDFIEYVAYTTIKEILVNYKK"
     gene            complement(468..1232)
                     /locus_tag="AC04VQ_07260"
     CDS             complement(468..1232)
                     /locus_tag="AC04VQ_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553890.1"
                     /translation="MKANYDDYIKMITKAQNICDIAIRKILKGRKRFRARNFRFRIVY
                     KANHLGSSQIEDGLCYFYCDPTNHLIPTKYALASLVWVAMQSKKRRNRPHPSVTAFDK
                     AKKAIGSKIFSGKIDAHHLSNEALFRFLIGYLYPRRMREYLFIYWYIKGAANEEEQFD
                     EIKYIESMLPQLKSIIDMEMLTYWGEILEQQCDKNWRDKYPELVVLGDEERFRENVIR
                     NAEYYTQLASTGYSIEAFAQMLCDFDNSQCKVMSNL"
     gene            complement(1407..1649)
                     /locus_tag="AC04VQ_07265"
     CDS             complement(1407..1649)
                     /locus_tag="AC04VQ_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553891.1"
                     /translation="MEKMTQEEKELIETRKEVLKALKDFVSIGKLDSPTMEDILDSIM
                     KPYIKLGSLFEYERDNTVEEKFLIQELNYYLDHLNK"
BASE COUNT          465 a          354 c          327 g          710 t
ORIGIN      
        1 tctcctgtcg attctcctat tttgttgagg cttcaaaaat aggaaaatta tttgataagg
       61 agagcaaaaa gttgcatttg ctctcctttt ggcaacgcgc atctgcaatt attgctaaaa
      121 acagagcggt tatttcttgt aattgacaag tatttccttg atggttgtat atgccacata
      181 ttcgataaaa tcataatctt ccgtcacttt tctatgattt ttattgtaac tgtagcgatg
      241 gccttttaac accaaataat cttcctcata ctgcacttca acagggctgt atttatcgat
      301 agaatattca tcttctttca cataacccaa gaggttgttc ccattaaggt gaaggtcttc
      361 aatctttttc ttttcattga gatgtttggc aagtattgcc tgaattgctt ccttattcat
      421 tgtttgtgtt tttaattttg tgtttgtaaa tcagttgttt attgttgcta taggttgctc
      481 attactttgc attggctgtt gtcgaaatcg cataacattt gggcaaatgc ctctatggaa
      541 taacctgttg aagcaagttg ggtataatac tcggcattgc gaatcacatt ttccctgaac
      601 ctttcttcat cgcccaacac aaccagttcg ggatacttgt ctctccagtt tttatcacat
      661 tgttgttcga gaatttcgcc ccaataggtt agcatttcca tatcaatgat tgatttgagt
      721 tgtggcaaca ttgactctat gtatttaatt tcgtcgaatt gctcttcttc gtttgctgca
      781 cctttgatat accaataaat gaataggtat tccctcatcc ttctaggata caaatagcca
      841 attaggaaac ggaataatgc ttcattgctt aaatgatgag catctatttt ccctgagaaa
      901 atcttcgaac caatagcttt tttggcttta tcaaaagctg ttaccgaagg atgtggacga
      961 ttacgacgct ttttgctttg cattgctacc catacaagcg aagctaaagc gtatttcgta
     1021 ggaatcagat ggttggtcgg gtcacagtaa aaatagcaca atccgtcttc aatttgggaa
     1081 cttcctaggt gatttgcttt atatacaatg cggaatctaa agtttcgtgc tctgaatctt
     1141 ttgcgccctt tcagaatttt ccgaatcgca atatcacaga tgttttgggc ttttgtaatc
     1201 atctttatgt agtcatcata atttgctttc atagtttcac cttttattat ttcttttctt
     1261 tcatttgtcc aaaggcgcac tggctgcatt ttaatggcta atcatgcaag atttgctggc
     1321 caggttttag aggtttttct ttgggttctc caattaccgg cttttctggc tttttaggct
     1381 cagtgtgttc gttgttgctc atgtttttac ttatttaaat ggtctaggta atagtttaat
     1441 tcctgaatca aaaacttctc ttctacagta ttatctcttt catattcaaa aagagaacct
     1501 aatttgatat atggtttcat aatgctgtcc aaaatgtctt ccattgttgg gctatcaagt
     1561 tttcctatgc taacaaaatc tttcagagct tttaacactt cttttcgtgt ttcaattaat
     1621 tctttttctt cttgggtcat tttttccatt tttgaattta acattacatt gcaagaagga
     1681 ggcaagcctc tctttggagg cctgcctcat ggttttaatc atttatgcgg ttctgtacaa
     1741 aacgccacac tgccattctg ctgcttttgg gcagccaggg tttgccacaa cgcttgaagc
     1801 tttgctgata actaattcta ctttcatttc agtaagattt agatggttat aggtgt
//
LOCUS       DBNCDC010000130         1845 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_60957_length_1845_cov_5.051397, whole genome shotgun sequence.
ACCESSION   DBNCDC010000130 DBNCDC010000000
VERSION     DBNCDC010000130.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60957_length_1845_cov_5.051397"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /gene="glmS"
                     /locus_tag="AC04VQ_07270"
     CDS             <1..247
                     /gene="glmS"
                     /locus_tag="AC04VQ_07270"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF002612.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HIO9553892.1"
                     /translation="DAILISSLYGQGELDCRGFGNKCKEAGLNDVLLYVGGNICIGKQ
                     NFEDVEERFKEMGFNRAYPPGTDIADTIEDLKKDLKC"
     gene            362..1720
                     /gene="glmL"
                     /locus_tag="AC04VQ_07275"
     CDS             362..1720
                     /gene="glmL"
                     /locus_tag="AC04VQ_07275"
                     /inference="COORDINATES: protein motif:HMM:NF040745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HIO9553893.1"
                     /translation="METDINEGLNNAVANLEKEIGKVDFAERYACSSAAGGLKIVSVG
                     LVPELTAKAAKMASLGAGGKVLDSFAFELNDEDAKSIAELKPEIVLLVGGTDGGNTEV
                     ILHNAKVIGDIEGDFAVIIAGNRNVAKECEDIISRNHKVYRCENVLPKFDQLNIEPVQ
                     EQIRDIFLDRIITAKGITKATSLVSGIVNPTPAAVLRGIKLLADGTKDEAGIGELVAV
                     DVGGATTDVYTVCSGKPTKGNVIYKGIPEPYAKRTVEGDIGMRYSIHGIVDAAGVDRI
                     AELSGLRIPEVEEMVDHLSKHTDEVPSDEKWEALDFALASMAIDTAVDRHAGNLEEVF
                     TAIGSTFMQTGKDLSNVKKIIVTGGSLIHTKRTDEIAANALFTLNSPNSLRPLSADVY
                     VDRKYIIASMGLLSEHYPDVALKILKESLQDLGTVKTKANISQSTEDDFEPVMDSGCH
                     GM"
BASE COUNT          565 a          306 c          447 g          527 t
ORIGIN      
        1 tgatgcaatt ttaatttctt cgctttatgg tcaaggtgag cttgactgca gaggttttgg
       61 taacaaatgt aaagaagccg gccttaatga cgtattgctt tatgttggcg gaaacatttg
      121 cattggtaag caaaacttcg aagatgttga agagcgcttc aaggagatgg gttttaatag
      181 ggcttatcct cctggaactg acatcgcaga cacaattgag gaccttaaga aagatcttaa
      241 gtgctaaggt gttaaattga aaccagtttt gctcatagat tttggaagca cctacacaaa
      301 ggttacggct gtagacgttg acgaagcgaa agtcttaggc acttctcaaa gttgaactac
      361 aatcgaaact gatattaatg aaggcttaaa caatgctgtt gctaatcttg aaaaagagat
      421 tggcaaggtt gattttgctg aacgttatgc atgtagtagt gctgctggtg gcttaaaaat
      481 tgtaagtgtt ggacttgtcc ctgaacttac agctaaagct gcgaagatgg cttctcttgg
      541 agctggaggt aaagttttag acagcttcgc cttcgagctt aatgatgaag atgcaaaatc
      601 tattgctgaa cttaaacctg agattgttct acttgttggt ggaactgacg gaggtaacac
      661 tgaagtcatt cttcacaatg ctaaagttat aggcgatatt gaaggggact tcgctgtaat
      721 tattgctggt aacaggaatg ttgctaaaga atgcgaagat attatttcta gaaatcacaa
      781 ggtttataga tgcgaaaatg ttcttccgaa gtttgatcag ctaaacattg aacctgttca
      841 agagcagatt cgcgatattt tccttgatag gattattact gcaaaaggca ttactaaagc
      901 aacaagctta gttagtggaa ttgttaaccc aacaccagct gctgttcttc gcggcattaa
      961 gcttttagct gatggcacaa aggacgaagc gggtattggc gagctcgtgg cagttgatgt
     1021 tggtggagca accaccgatg tttacaccgt atgttctggc aaaccaacaa aaggtaatgt
     1081 aatttacaaa ggtattccag agccttacgc caaaagaacg gttgaaggtg acattggaat
     1141 gcgctatagc attcatggca ttgttgatgc tgccggcgtt gacagaattg cagagttgtc
     1201 tggacttcgt attcctgaag tcgaagaaat ggttgaccat ttaagtaagc acactgacga
     1261 ggttccatct gatgaaaagt gggaagcgct tgattttgcg cttgctagca tggcaattga
     1321 cactgctgtg gatagacatg ctggcaactt agaagaggta tttactgcga ttggttcaac
     1381 ctttatgcaa actggtaaag atttgagcaa tgtcaaaaag ataattgtaa ctggcggaag
     1441 tttaattcac acaaagcgca ctgatgaaat tgctgcaaac gctttattta cattaaactc
     1501 gccaaatagc ttacgcccat tgagtgcaga tgtttatgtt gacaggaaat atatcattgc
     1561 aagcatgggc ttgttgagcg aacattaccc ggatgttgca ttgaagatat tgaaagaaag
     1621 cttgcaagat ttgggaaccg ttaaaacaaa ggctaacatt agtcagtcga ccgaagacga
     1681 cttcgagcct gtaatggata gtggttgtca cggtatgtag ttattgaaaa taggttataa
     1741 ccttattgga tattattaga gaggagatca aatggatgta gcaaacaaaa aaattcctga
     1801 gggcgatttt gaaaaagagc gccaagaggt cttagctacc tggca
//
LOCUS       DBNCDC010000131         1806 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_63327_length_1806_cov_4.478584, whole genome shotgun sequence.
ACCESSION   DBNCDC010000131 DBNCDC010000000
VERSION     DBNCDC010000131.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63327_length_1806_cov_4.478584"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /gene="queA"
                     /locus_tag="AC04VQ_07280"
     CDS             <1..864
                     /gene="queA"
                     /locus_tag="AC04VQ_07280"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF014592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIO9553894.1"
                     /translation="VRNNTKFLPARIFGVKEGTGAHVEILLLKQIEGDTWECLAGNAK
                     PIKVGSIIDFNNGQLKAECLEVRDEGIRIMNFKYNGIFLEILNEIGAMPLPPYIKKKC
                     EDNSQYQTVFAKVYGSAAAPTAGFHFTEELIKKCEEKGVEFLDVTLNIGLGTFKPVKE
                     KTVEAHKMHTEHYEISQEVADRLNLAKKEGRRIIALGTTSTRTLEANMQKFGEFRADK
                     DDTCIFIKPGYEYKAIDALITNFHLPKSTLIMLVSALMGREFTLDCYKHAVEEKYRFF
                     SFGDAMFIYGK"
     gene            854..1510
                     /locus_tag="AC04VQ_07285"
     CDS             854..1510
                     /locus_tag="AC04VQ_07285"
                     /inference="COORDINATES: protein motif:HMM:NF014707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HIO9553895.1"
                     /translation="MANKINYYKQSIEAIEKLPKTGKKPSLLLHACCGPCSTFPLTFL
                     CPHFDVTIFFNNSNIYPSEEYYRRLNELKKFLEFFKRDYGFDVKIIETLYKNDEYNLN
                     LEKYADQPEGQTRCFLCYEKRMNEAFKYANENGFDYFTTVMTISRQKDSQKLNEIGGK
                     LQKSYGKTQYFHSDFKKNKGIDIAREMRIHYDLYQQLYCGCKYTYEKGLKKAAEKEQK
                     "
BASE COUNT          642 a          309 c          341 g          514 t
ORIGIN      
        1 gttcgaaata atactaaatt tttgccggcc agaatttttg gtgtaaaaga aggtactggg
       61 gcccatgtag aaatccttct tttgaaacaa attgaaggtg atacttggga gtgtttagca
      121 ggtaatgcaa aaccaataaa agttggctca ataatcgact tcaataatgg ccaattaaaa
      181 gccgaatgct tggaggttcg tgacgaagga attcgtatca tgaatttcaa atataatggg
      241 attttcctgg agattttgaa cgaaatcggc gctatgccac ttcctccata catcaagaaa
      301 aagtgtgaag acaactcaca atatcaaaca gtttttgcta aagtatatgg cagcgcagca
      361 gcacctactg caggcttcca ttttactgaa gaattaatca aaaaatgtga agaaaaaggt
      421 gttgagtttt tagacgttac tttgaacatc ggattaggca cttttaaacc tgtaaaagag
      481 aagactgttg aagctcacaa aatgcacact gaacactacg aaatttctca agaagttgca
      541 gaccgtttga accttgcaaa aaaggaagga agacgcatta ttgcgttagg cacaacttca
      601 accagaactt tggaagcaaa catgcaaaaa tttggtgagt ttagagcaga taaagacgat
      661 acttgcattt ttattaagcc aggttatgag tacaaggcca ttgatgcgct cattacaaac
      721 ttccatttgc ctaaatctac tttaattatg ctcgtttcag ctcttatggg cagagaattc
      781 actttagatt gttataaaca cgcagttgaa gagaaatata gattcttttc ctttggagac
      841 gcaatgttta tttatggcaa ataagattaa ctactacaag caatcgattg aggctattga
      901 aaagttgcca aaaacaggta aaaaaccaag cctgcttttg catgcttgtt gtggtccttg
      961 ttcgacattt ccactcacat ttttgtgtcc tcattttgac gttacaatct tctttaacaa
     1021 ctcaaatatt tacccgtcag aagagtacta tagacgccta aatgagctca aaaaattcct
     1081 agaattcttt aaaagagact atggttttga tgtaaaaatt atcgaaactt tatacaaaaa
     1141 tgatgaatat aatttaaatc tcgaaaagta cgctgatcaa ccagaaggcc aaactcgctg
     1201 ttttttgtgc tatgaaaagc gtatgaatga agcatttaaa tatgccaacg aaaacggctt
     1261 tgattacttc actacagtaa tgacaatttc gcgtcaaaaa gactctcaaa aactcaatga
     1321 aattggcgga aaattgcaaa aatcctatgg aaaaacccaa tattttcatt ctgatttcaa
     1381 gaagaacaaa ggcatcgata ttgctcgtga aatgcgcatt cattatgacc tttatcaaca
     1441 gctttattgt gggtgcaaat acacctacga aaaaggccta aaaaaggcag cagaaaaaga
     1501 gcaaaaataa acaaaaaatt aacaaaaaaa tgatagaaaa ataggggttt ggagcccctt
     1561 ttttcgtatg cattatctat taaattaata aattttcgct tgcatatatt atatataggc
     1621 gtattatata acacgttaac gtttgcgttg acatgcgagg ttgccgacac accgtcaggc
     1681 gttgtcatga gaaaggttgc cggggaattc gcatcgagca gttcgttaaa aagcctgaaa
     1741 gaaaaattag gaggaaaatt catggcaaaa gtaaaaacca ttagggttcg cttacaagct
     1801 tatgat
//
LOCUS       DBNCDC010000132         1794 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_64084_length_1794_cov_4.627947, whole genome shotgun sequence.
ACCESSION   DBNCDC010000132 DBNCDC010000000
VERSION     DBNCDC010000132.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64084_length_1794_cov_4.627947"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(54..770)
                     /gene="rnc"
                     /locus_tag="AC04VQ_07290"
     CDS             complement(54..770)
                     /gene="rnc"
                     /locus_tag="AC04VQ_07290"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIO9553896.1"
                     /translation="MENPIDINDHKDRLAKAEKILDYEFKDKNILLKALTHPSAVQGS
                     STEHSYERYEFLGDAILEAAVSYSIFRKYEDMDEGKLTHMRVAIVSGDNLSKVAEELG
                     LSDLIIFGKSEESTGKRGMKRALENVLEAIVAAVFLDGGEVNCWVFIVNKVISKMIDD
                     DRLAIEGNPKSVLQELMQINHDTPIYNIVEESGPAHNKSFVAEVLVDNKVLATGEGSS
                     KKEAEVNAALNALQKRGENQ"
     gene            complement(770..>1794)
                     /gene="plsX"
                     /locus_tag="AC04VQ_07295"
     CDS             complement(770..>1794)
                     /gene="plsX"
                     /locus_tag="AC04VQ_07295"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012798147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIO9553897.1"
                     /translation="RSFRMSSGKVLIAVDALGADNAPSVVLDGVKQALEQDKSLNILL
                     CGPKDVVDDFVSTQERCEAVICSEEIKMDEHPANAVRKKKDSTIVIGCKCVKEGKADG
                     FYSAGNTGACLAAATLVTGRIKGVLRPMLATNIPTQEPGKSTLFCDLGANADCKPENL
                     LQFGIMASSYIKAVKSIKNPKVALLNIGEEDSKGSQLAQETNALMKDKLKGFSGNAEG
                     RDITNGKFDAVITDGFTGNVVLKSMEGTLKMTFGILKATFMSSLKSKIGAFLVKGSIK
                     KMVKDLDPESIGAALLFGVKGACCVGHGNSSAKAICNGILNTAEFARNKVADKIEAEI
                     KKAGIK"
BASE COUNT          496 a          419 c          282 g          597 t
ORIGIN      
        1 taccttatca gcaaacgatt taaaaccttt catttcaagt cgctttaaat acattattga
       61 ttttctcccc gtttttgaag cgcgtttaaa gcagcattaa cctcagcttc tttttttgaa
      121 ctgccttccc ctgttgccaa aactttattg tcaaccaaaa cttccgcaac aaaggatttg
      181 ttatgggctg gaccgctttc ctcaactata ttatatattg gagtatcgtg attgatttgc
      241 attaattctt gaagaacgct ttttgggttc ccttcgatag caagtctatc atcatcgatc
      301 attttgctta ttactttgtt tactataaaa acccagcagt ttacttcgcc accatctaaa
      361 aatacagcag caacgatagc ctccaagaca ttttccaagg ctcttttcat gcctcgtttg
      421 cctgtgctct cttcagactt accgaatatt attaagtcgg atagacctaa ctcttcagca
      481 actttagata aattatctcc agaaacaatc gcaactctca tatgagtgag cttgccttcg
      541 tccatgtcct cgtattttct aaatatcgaa tacgaaacag ctgcttcaag tatagcatca
      601 cccaaaaatt cataacgctc gtaagaatgc tctgtcgaac taccttgaac ggccgacgga
      661 tgagttaatg ctttaagcaa aatgttcttg tctttaaatt catagtcaag aattttttca
      721 gcttttgcaa gtctatcttt atggtcattt atgtcgatag gattttccac tatttgattc
      781 ctgctttttt aatttctgcc tctattttat cagctacttt atttcttgca aattctgcag
      841 tatttaaaat tccgttacag attgctttcg cggacgaatt gccgtgacca acacaacaag
      901 ctcccttaac tccgaagaga agcgcagctc caattgattc cggatctaaa tctttaacca
      961 ttttcttaat gcttccctta accaaaaaag ctccaatttt tgattttaga ctggacatga
     1021 atgtagcttt taaaatgcca aaggtcattt taagtgtgcc ttccatgctt tttaaaacaa
     1081 catttccagt aaatccgtca gtaatcaccg cgtcaaattt accatttgtg atgtctcttc
     1141 cttcggcgtt tccgctaaag cctttaagct tgtctttcat aagggcatta gtttcttgag
     1201 caagctgact tcccttgctg tcttcttcgc caatatttag cagagcaact tttggatttt
     1261 ttatgctctt aacagccttt atgtagcttg aagccataat gccaaactgc aataaatttt
     1321 caggtttaca gtcagcattg gcacctaaat cgcaaaatag tgtagatttt ccaggttctt
     1381 gtgttgggat gtttgtagca agcataggac gaagaacacc ttttatcctg cctgttacaa
     1441 gcgtagctgc agccaaacaa gctccagtgt tacctgccga ataaaagcca tcagccttgc
     1501 cttccttgac acacttgcaa cctataacaa tagttgaatc tttctttttt cgaacagcat
     1561 tagcaggatg ttcatccatt ttaatctctt cgctgcaaat aacagcttca caacgctcct
     1621 gcgtactaac gaagtcatca acaacatcct ttggaccgca aagcaaaata tttaatgatt
     1681 tatcctgctc aagggcctgt ttgacgccat caagaacaac gcttggagca ttatccgctc
     1741 caagcgcgtc tactgcaatt aaaactttcc cgcttgacat gcgaaaagac ctaa
//
LOCUS       DBNCDC010000133         1639 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_75150_length_1639_cov_7.035985, whole genome shotgun sequence.
ACCESSION   DBNCDC010000133 DBNCDC010000000
VERSION     DBNCDC010000133.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75150_length_1639_cov_7.035985"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="AC04VQ_07300"
     CDS             complement(<1..149)
                     /locus_tag="AC04VQ_07300"
                     /inference="COORDINATES: protein motif:HMM:NF024575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIO9553898.1"
                     /translation="MRNYFKDIVGQDLAIKILRSELSEDRVSHAYLFCGPLGCGKLNC
                     AKAFA"
     gene            complement(160..1065)
                     /locus_tag="AC04VQ_07305"
                     /pseudo
     CDS             complement(160..1065)
                     /locus_tag="AC04VQ_07305"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     gene            complement(1233..1568)
                     /locus_tag="AC04VQ_07310"
     CDS             complement(1233..1568)
                     /locus_tag="AC04VQ_07310"
                     /inference="COORDINATES: protein motif:HMM:NF016027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIO9553899.1"
                     /translation="MHFVFCVGQDEAYANKLSKLANNVNASNFTILTYTDKLPQLMSI
                     ADIAIVKPGGLIVSECINMNLPMILIGKAYAQENLNRRFLISNGAAVHATTYKGVINF
                     LCDIFTYPN"
BASE COUNT          549 a          323 c          227 g          540 t
ORIGIN      
        1 ttagcaaaag ccttcgcaca attcaacttc ccacaaccaa gtggaccgca aaataaatac
       61 gcatgcgaga ctctatcttc actaagttca gaacgaagaa tttttatcgc caaatcttga
      121 ccaacaatat ctttaaaata attacgcatg tttaattagt cattgcaaaa tatcatcata
      181 aacaacatct tgatcaaact cgtttaatat ttcgtgcctg tcttcaggat acaactttaa
      241 tgtaacatct ttaattccag ctttaatata agacttgttg acaacttttg gagcataccc
      301 aaagtcaccc acaggatctt gttcgccagc gcatataaaa actggcaaat ttttagggat
      361 cttatttatg tttttcttct tttcgataaa agacatcaaa tcaaacatat taaagaaccc
      421 attaagtgta aaaacatatc cacaataagg atcattttga tagcgagtgg cttgtttatc
      481 atctctgcat agtcattcgt gaccagtaac aataggctga aaacgtttgt tataacctcc
      541 aacagctaaa ttgttgataa aatttgagcg atggcgccat tctttaaata ttgaaattag
      601 cttgcagaag aattttgcaa aaccaagagc aatctggggc tgatttccag ttcccataac
      661 aattgctcca tctaaagttt cgccataagt ctcaataaac ccgcgacata aaaaacttcc
      721 catactatgt ccaagcaaaa aatatggaag ctttggataa tcttgcttga tagtatcttg
      781 aagttttttc atatccttaa gaacagtttg atatccattc ttctctgcgc aataacctaa
      841 atcggcttta ttggcagcag tttttccgtg gcctaaatga tcttcgccac aaactaaaaa
      901 tccatgctta ttcaaaaact tggcaaaatc ttcgtaacgc ataatgtgtt ctgccattcc
      961 atgtgcaatt tgtaaaatat atttaggttc accatctggc agccacttaa cagcatgaat
     1021 aaaattcttt ccatcgttag atttgtaatt aaattcttcc accataatcc ctcctatctt
     1081 gaatgaatag gcttctttcc taagtagata ttatttcaca acttaggttt ttctatatga
     1141 ctttcatcaa tgtgttcaac cacttctgaa cagatttttt ttgacgaatt tgctcttcta
     1201 attttagaaa gaccaaattt taaacttgca tatcaatttg gatatgtaaa aatatcacac
     1261 aagaaattta ttacaccctt atatgttgtc gcatgaacgg cggctccatt tgaaattaaa
     1321 aatcgacgat taagattttc ttgtgcatat gcttttccaa ttaatatcat tggcaaattc
     1381 atatttatgc attcactaac aataagaccg ccaggtttga caatagcaat atctgcaatt
     1441 gacatcaatt gaggcaattt atctgtgtag gtcaagattg taaagttcga agcattaaca
     1501 ttgttcgcca attttgaaag tttgtttgca taagcttcat cttgaccaac acaaaaaaca
     1561 aaatgcaatc aatccatctt cgaaaaaaaa cttaaacatg catttaaagt ctttctcata
     1621 tttttataag gtccagaat
//
LOCUS       DBNCDC010000134         1588 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_79455_length_1588_cov_6.318330, whole genome shotgun sequence.
ACCESSION   DBNCDC010000134 DBNCDC010000000
VERSION     DBNCDC010000134.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79455_length_1588_cov_6.318330"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            212..1551
                     /locus_tag="AC04VQ_07315"
     CDS             join(212..656,656..1551)
                     /locus_tag="AC04VQ_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611142.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIO9553900.1"
                     /translation="MPKYTYEEKKEAIDRVLKEGMSPNESAKILGCRHSTVEIWVDNY
                     KAHGEASLHKGISSYDGDFKLHVVEYMHANELSYRAAAIKFGVKTSNNVAKWERIYYE
                     EGPEALYIDRRGRPRKMREEDLDEQTKEDLIAEVQRLRMENEYLKKFESLSSGKDCPR
                     EWERAAIINELRQKYKLSDLLAYDGMPRSSFYFYLKQMKRPDKYAELKEVIKQIFAEN
                     KGRYGYRRITLELHNRGYFVNHKTVQRLMRKCNLKCEIRKKKYRSYKGEIGKVAPNIL
                     DRNFKATKPNQKWVTDVTEFAVGDDKVYLSPILDLFNGEIISYNISLRPTFNQTMDML
                     DQAFEKIPDNTNLILHSDQGWQYQMKRYQHRLKEKGIVQSMSRKGNCLDNSVMENFFG
                     LLKTEMFYKHQFDSTNQLIDEIEKYIDYYNNVRIKAKLNGLSPVQYRNQSMLVA"
BASE COUNT          592 a          260 c          324 g          412 t
ORIGIN      
        1 tgaaaagggt gaggcagacg ttaaaatcct ccacactcca gacaagtggt tcggcgtaac
       61 atatgctgat gacaaggatg ctgttaaggc agcatttaaa gatcttgttg caaagggcgt
      121 atacccagaa aagattaact aagataaaaa ttaaagaggt agcaaaaatg aactgacccc
      181 caaatgttag acaacatttg ggggtctttt tatgcctaaa tacacatatg aagaaaagaa
      241 agaagcaatc gacagagtgc ttaaagaggg aatgtcacca aatgaaagtg caaaaatcct
      301 tggatgtaga cattctactg ttgaaatatg ggtagataat tataaagctc atggtgaggc
      361 aagtttgcac aagggtataa gtagttacga tggtgatttt aaactccacg tggtagaata
      421 tatgcatgct aatgaacttt cttatcgtgc tgccgcaata aaatttggag taaaaacttc
      481 taataatgtt gcaaaatggg aacgcattta ttatgaagaa ggtccagaag cgctttatat
      541 tgatcgacga gggagaccaa gaaaaatgcg cgaagaagat ttagacgaac agacaaaaga
      601 agatttgatt gccgaagttc aacgtcttcg aatggagaat gaatatctaa aaaaattgag
      661 agccttagtt caggaaagga ttgcccgaga gaatgggaaa gagccgccat catcaacgaa
      721 ttaaggcaga aatacaaact tagcgattta ctcgcttatg atggcatgcc tagaagctct
      781 ttctacttct atctaaagca aatgaaacgc cctgacaagt acgctgaatt aaaggaagtt
      841 attaagcaaa tatttgctga aaacaaaggt agatatggct acagaagaat tacacttgag
      901 ctgcacaaca gaggatattt tgtcaatcac aaaacggtgc aaaggcttat gcgaaaatgc
      961 aatttgaagt gtgaaatccg aaaaaaaaaa tatcgttcat acaaaggcga aattggaaaa
     1021 gttgcgccta atatactcga ccgcaacttt aaagcaacta aacctaatca aaaatgggta
     1081 actgatgtca cagagtttgc tgttggtgac gacaaggtct atttatcacc gattcttgat
     1141 ttgtttaatg gagaaatcat tagttacaat atctctcttc gtccaacctt taatcaaacg
     1201 atggatatgc tggatcaagc ttttgaaaag ataccagaca atacgaattt aattcttcat
     1261 tcagatcaag gttggcaata ccaaatgaaa cgatatcagc accgcttaaa agaaaaagga
     1321 attgttcaaa gcatgtcgcg caaaggcaac tgcctagaca attccgttat ggaaaacttc
     1381 tttggtctac ttaaaacaga aatgttttac aagcatcagt ttgattcaac aaatcaactg
     1441 attgatgaaa ttgaaaaata tattgattat tacaacaacg ttaggattaa agcaaaacta
     1501 aatggactga gtcctgtaca atacagaaat cagtccatgt tagtagctta gattaaatta
     1561 aagtgtccaa cgtttggggt tcacttca
//
LOCUS       DBNCDC010000135         1581 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_80097_length_1581_cov_5.437746, whole genome shotgun sequence.
ACCESSION   DBNCDC010000135 DBNCDC010000000
VERSION     DBNCDC010000135.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80097_length_1581_cov_5.437746"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..80
                     /locus_tag="AC04VQ_07320"
     CDS             <1..80
                     /locus_tag="AC04VQ_07320"
                     /inference="COORDINATES: protein motif:HMM:NF022048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553901.1"
                     /translation="TCIARNPKCHACPLAKLCPSKQTIV"
     gene            complement(77..385)
                     /locus_tag="AC04VQ_07325"
     CDS             complement(77..385)
                     /locus_tag="AC04VQ_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553902.1"
                     /translation="MVKDICFNNINATSENGCFISGDTPTKVSGVYFNAVNINLYKKT
                     NYQIGVYDKRPCVGEGFIYDKTYGIYVDKATGVITNAFLVQFDPSFPQELYGGDKHGF
                     "
     gene            complement(395..742)
                     /locus_tag="AC04VQ_07330"
     CDS             complement(395..742)
                     /locus_tag="AC04VQ_07330"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014652.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HIO9553903.1"
                     /translation="MLDALDSTDQLPRTIFYNLNPRDNDLLVALAGSFQDGKTKGKIQ
                     YGSAWWFLDNINGMIRQLDSVANNGMLSTSIGMLTDSRSFLSFPRHEYYRRILCNYIG
                     EKVENGEFPNDIK"
     gene            complement(915..1574)
                     /locus_tag="AC04VQ_07335"
                     /pseudo
     CDS             complement(915..1574)
                     /locus_tag="AC04VQ_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
BASE COUNT          493 a          343 c          236 g          509 t
ORIGIN      
        1 aaacttgcat agcaagaaac ccaaagtgtc atgcttgtcc ccttgcaaaa ctttgtccta
       61 gcaaacaaac tatagtttaa aaaccgtgct tgtccccacc gtaaagttcc tgcgggaagg
      121 atgggtcaaa ctgaacaagg aatgcattag ttattacgcc tgttgcttta tcaacataga
      181 tcccataagt cttatcataa ataaaacctt cgccaacaca tgggcgctta tcataaacgc
      241 caatttgata attggttttc ttatataagt taatatttac agcattaaaa tatactccac
      301 taaccttagt tggagtatct ccactaataa agcatccatt ttcagaagtg gcgtttatat
      361 tgttaaagca aatatcctta accatgccag aaagctattt gatgtcattt ggaaattcgc
      421 cgttttcgac cttttctcca atataattac acaatattct acggtaatat tcatgtctcg
      481 ggaatgacaa gaagcttcgc gaatcagtaa gcattccaat agatgtgcta agcattccgt
      541 tgttagcaac actatcaagc tgacggatca ttccgttaat gttatccaag aaccaccacg
      601 cactgccgta ttgaatctta ccctttgttt ttccgtcttg aaaacttcca gccagtgcaa
      661 ctaataagtc gttatctctt gggtttaagt tataaaaaat tgttcttgga agttgatcag
      721 tagagtcaag agcatcaagt atgcaactgt tgagctaaat tgtactcgtg atataatgaa
      781 catatgtatt taatcctgca aattcgaaat taaaatcagg cctaaaactt agcataactt
      841 taaattcaat aacaccttag cagtatcgtt atttaataag aaattttcat ccataaaatt
      901 tttcatgaat tctcctatct tgaaactctt ccggagccat caccttcagc aagtttaata
      961 acttcgtcaa cattaacatg attaaagtcg ccttcaatag agtgctttaa agcacttgct
     1021 gcaactgcaa actcaattgc aaattcatcg tttttatttt gcaatagacc ataaataagt
     1081 ccagcaccga aactgtctcc cccgccaact ctatccacta tatgaagctt gtatttctta
     1141 ctaaacaagg ctttatcttc ctttgtgttg tagagcattg cacttcaatt attatccgaa
     1201 gcacttaagc tctcacgcaa agtaatagca acatacttaa agttaaattt atcatgtagc
     1261 tgctttgcaa cgtcaatata gccttcttta ttaagctctc cactttcaac attagtacct
     1321 ctcgacttaa ttccgaagac cttctctgcg tcttcttcgt tgccaataca aacatcaaca
     1381 tactccatta acttagacat tgttttattt gcatcttcgc ttgaccagag tttctttctg
     1441 aaatttaaat cagcagatat tgtaagccct gccttttttg cttgcttaca agcttcaagg
     1501 cagatatcag cacaagactt tgatagagcc ggagtaattc cggtaaaatg gaaccagtcg
     1561 ctgccttcaa aaattgtgtt c
//
LOCUS       DBNCDC010000136         1577 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_80535_length_1577_cov_1.925099, whole genome shotgun sequence.
ACCESSION   DBNCDC010000136 DBNCDC010000000
VERSION     DBNCDC010000136.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80535_length_1577_cov_1.925099"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(21..146)
                     /locus_tag="AC04VQ_07340"
     CDS             complement(21..146)
                     /locus_tag="AC04VQ_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553904.1"
                     /translation="MFYGCVSLKGQENTKFLSYHTNIEFAKVDGLEGRPGYFTGI"
     gene            complement(174..812)
                     /locus_tag="AC04VQ_07345"
     CDS             complement(174..812)
                     /locus_tag="AC04VQ_07345"
                     /inference="COORDINATES: protein motif:HMM:NF015347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HIO9553905.1"
                     /translation="MSNAKSCDSMFACYNPSYPVHQQAEKEHPVKEQENAKIDDPYAF
                     KLNLPKTLTFGEGSTTRQMFMLYFPHELDISGYDVSKVTDMTRMFAMCRADRIDTSTW
                     DTSACENMEGMFLAASAKVIDMSSWDYHNVKNMRFMFMAYSNEDVSTAIYFPEDNTMR
                     AAKGCDGMGMFALCDSKALMLYGLNIEGFSNIDYMFAGCTNLERINVGVETD"
     gene            complement(1128..1367)
                     /locus_tag="AC04VQ_07350"
     CDS             complement(1128..1367)
                     /locus_tag="AC04VQ_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553906.1"
                     /translation="MMFLGYGAFARIYADNDKLIPCPFSGKQLKLPTALPSAPKTASE
                     GYCSASWMFAFASIFDASDSEIAGALKSTTLDLSG"
     gene            complement(1395..>1577)
                     /locus_tag="AC04VQ_07355"
     CDS             complement(1395..>1577)
                     /locus_tag="AC04VQ_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9553907.1"
                     /translation="FKLIGDCDTRYMFSFFIEFPGISDKKYGNFDLQGIDVGELTNFK
                     SMFAFGLGIFNFDLSK"
BASE COUNT          503 a          334 c          239 g          501 t
ORIGIN      
        1 tcaacaggct ctttaagtgt tcatattcct gtaaaatagc ctggtctgcc ttcaaggcca
       61 tcaactttag caaattcaat gttagtatga taagacaaga actttgtatt ctcttgtccc
      121 ttaaggctaa cacagccgta gaacatataa gagccagcaa taccatattt agttcaatca
      181 gtctctactc caacattgat cctttcaaga tttgtgcatc cggcaaacat ataatcaata
      241 ttgctaaaac cttcaatatt aagtccgtac agcattagtg cctttgagtc acacaaagca
      301 aacatgccca taccatcaca gcctttagcg gctctcatag tattgtcttc agggaagtaa
      361 atagctgttg aaacgtcttc gttgctataa gccataaaca taaatctcat attctttaca
      421 ttatggtaat cccaacttga catatcaatg actttcgcac ttgcagctaa aaacatacct
      481 tccatatttt cacatgctga ggtatcccat gtactggtat caatcctatc agccctacac
      541 atggcaaaca ttcttgtcat atcagtaacc ttagatacat cgtatccaga aatatcaagt
      601 tcgtgaggga aataaagcat gaacatttgt cttgtcgtgc ttccctctcc aaaagttaat
      661 gtttttggca aattaagctt aaatgcataa gggtcatcaa tcttagcatt ttcctgttct
      721 ttaactggat gttccttttc tgcttgttga tgtacaggat acgaagggtt ataacatgca
      781 aacatagaat cacaagattt agcattgctc aaatctaatt tcggagcatt tacattacaa
      841 ttcgcaaatg caaaagaacc tgtcagatct gcgccactag caaacttcgt atcgttttca
      901 gaagaaaact taatttcaac aagcttcagt ggattgactt tttcggcagt taataattgt
      961 ttattttgtg taaagtgttg atttatttct tttcttttta attcatcaaa ctgccatcaa
     1021 atattattaa taatttgccc taaattatct cacgcttgag aataagctac aattgaaaaa
     1081 ttgtatgaaa agaacatatg gtcagccttt ttgataccgc caaaatctca accgctcaaa
     1141 tctaatgttg tgctttttaa tgcccccgct atttcactat ctgaagcatc aaatatactt
     1201 gcaaaagcaa acatccacga agcgctgcaa tatccttccg aagctgtttt tggtgccgaa
     1261 ggcaaggccg taggtaactt taactgcttt ccactgaatg gacatgggat aagcttgtca
     1321 ttgtctgcat aaattcttgc aaacgcaccg taacctaaga acatcatgtc caaattcttt
     1381 gctttagaaa tatctcattt tgacaaatca aagttaaaga ttcccaaacc aaaagcaaac
     1441 atggacttaa agttagttaa ttcgccaacg tcaattcctt gcaaatcgaa gttcccatat
     1501 tttttatcac taataccagg aaattcgatg aaaaagctaa acatgtatct tgtgtcgcaa
     1561 tcgcctatta gcttaaa
//
LOCUS       DBNCDC010000137         1504 bp    DNA     linear   ENV 28-MAR-2026
DEFINITION  MAG TPA_asm: Eggerthellaceae bacterium isolate MGR18
            NODE_87353_length_1504_cov_4.175293, whole genome shotgun sequence.
ACCESSION   DBNCDC010000137 DBNCDC010000000
VERSION     DBNCDC010000137.1
DBLINK      BioProject: PRJNA1366607
            BioSample: SAMN53317960
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eggerthellaceae bacterium (feces metagenome)
  ORGANISM  Eggerthellaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Coriobacteriia;
            Eggerthellales; Eggerthellaceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Parks,D.H.
  TITLE     First report of stop codon reassignment to tryptophan in members of
            the bacterial phylum Actinomycetota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2026) Department of Chemistry and Bioscience,
            Aalborg University, Fredrik Bajers Vej 7H, Aalborg 9220, Denmark
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Aviary v. 0.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 9.3x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/23/2026 11:37:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,475
            CDSs (total)                      :: 1,443
            Genes (coding)                    :: 1,247
            CDSs (with protein)               :: 1,247
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 0 of 196
            Pseudo Genes (incomplete)         :: 75 of 196
            Pseudo Genes (internal stop)      :: 147 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Eggerthellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87353_length_1504_cov_4.175293"
                     /isolate="MGR18"
                     /host="Equus ferus"
                     /db_xref="taxon:1972561"
                     /environmental_sample
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="AC04VQ_07360"
     CDS             complement(<1..226)
                     /locus_tag="AC04VQ_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIO9553908.1"
                     /translation="MADEKQNIIEDDPYKVRLNKRAAMIDAGENPYGEAFAFTHHVAD
                     MDAKYGKLEDGQITNDEVKVAGRILSKRGQG"
     gene            complement(228..707)
                     /gene="greA"
                     /locus_tag="AC04VQ_07365"
     CDS             complement(228..707)
                     /gene="greA"
                     /locus_tag="AC04VQ_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015778865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIO9553909.1"
                     /translation="MPEEEEIILTKEGKEKLEAELYQLEHEKRAEVSARIKVAREFGD
                     ISENSEYDDAKNEQAHVEKRISEINHILQNAKIVKKPAKNSDKIGVGSTVKVNMGGKQ
                     NTFTIVGGSESDIAHGKISNTSPVGKALCGHKKGDKVETEGPTGKKIEIKILDVKHD"
     gene            complement(780..1133)
                     /locus_tag="AC04VQ_07370"
     CDS             complement(780..1133)
                     /locus_tag="AC04VQ_07370"
                     /inference="COORDINATES: protein motif:HMM:NF014470.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033281 - TAT protein transport complex
                     [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA/TatB"
                     /protein_id="HIO9553910.1"
                     /translation="MISQVMLIGLGPGEIIVILVVAFVLLIILGPKNLPKLGNSIGKG
                     IKGLRKGMQSEEEKKDDDEIIVETEEVEEKPKKKTTTAKKPAAKKAPAKKTAAKKPAA
                     KSTTTKKKVVRKKSE"
     gene            complement(1219..>1504)
                     /locus_tag="AC04VQ_07375"
     CDS             complement(1219..>1504)
                     /locus_tag="AC04VQ_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012797635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta-related
                     protein"
                     /protein_id="HIO9553911.1"
                     /translation="FPGISMDVAFTVDKSVTHEMMMQRITSAGGNLLKSVKLFDVFES
                     EKHLGKDKKSMAYSLEYNNPQKTLTSEEVEAVHSKLIEKVCKSTGAEVRK"
BASE COUNT          359 a          346 c          261 g          538 t
ORIGIN      
        1 taccttggcc tcgctttgaa agaatgcgcc cggcaacctt aacttcgtca tttgtaattt
       61 ggccatcttc aagcttgccg tacttcgcgt ccatatctgc aacatgatga gtgaaggcaa
      121 aagcctcgcc ataagggttc tcacctgcat cgatcattgc agcacgcttg tttaggcgta
      181 ctttatatgg atcatcttca attatgtttt gtttttcgtc agccatatta atcgtgtttt
      241 acatccagaa ttttaatttc aatttttttc cctgtagggc cttcagtttc aaccttgtca
      301 ccttttttgt gaccgcataa tgctttacca accggcgaag tatttgaaat cttgccatgt
      361 gcaatgtcgc tctcagagcc acctacaatt gtaaaagtgt tctgctttcc acccatatta
      421 acttttacag tgctgccaac cccgatcttg tcagagttct tagcaggctt tttaacaatt
      481 ttggcgtttt gtaaaatgtg gttaatttca ctgatgcgct tttcaacatg ggcttgttcg
      541 tttttggcat cgtcatattc agaattttca gaaatgtcac caaactcacg agcaacctta
      601 atgcgcgcac taacctcagc acgtttttca tgctccaatt gataaagttc agcctcaagc
      661 ttttctttac cttctttggt taaaattatt tcttcttcct ctggcataac tctccaatcc
      721 tcatgcaata aaatatatcg gcagcaatgc cgccaaatga attttttatc gcctaaattt
      781 tattcggatt ttttgcggac gacttttttc ttagttgtag ttgatttagc agcaggtttt
      841 ttggcagcag tctttttagc tggagccttc tttgctgcag gtttttttgc agttgtagtc
      901 tttttcttag gcttctcttc tacttcttca gtctctacaa taatttcatc atcgtctttt
      961 ttctcttctt cgctttgcat tcctttacga agacctttaa ttcccttacc aattgaattg
     1021 ccaagttttg gaaggttctt tggtcctaaa ataatcaaaa gaacaaaagc aacaactaaa
     1081 atgacgataa tttcgcctgg tccaagacct attaacatta cttgagaaat catctctttt
     1141 ctccttttac tcgcccgctt aatctcgagt tttgcgtata ttagcacaaa gttgttcgca
     1201 ttacaaacaa aattaaaatt acttgcgaac ctcggcgcca gtggatttgc agactttttc
     1261 gatgagcttg ctgtggacgg cttcaacctc ttcgcttgta agagtttttt gtgggttgtt
     1321 gtattcaagc gaataagcca tgctcttttt gtccttgcct aaatgcttct cgctctcgaa
     1381 aacgtcgaag agctttacgc tctttaacaa gttgccgcct gccgaagtaa tgcgctgcat
     1441 catcatctcg tgagtaacgc ttttatcaac ggtgaaagct acatccatcg aaatgcctgg
     1501 gaac
//
